AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007765

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007765 MtrunA17_Chr2g0296221 75.931 403 91 2 1 397 25 427 0.0 619
MsaG007765 MtrunA17_Chr2g0296191 74.935 387 91 2 1 381 608 994 0.0 586
MsaG007765 MtrunA17_Chr2g0296191 60.165 364 133 4 20 383 158 509 8.97e-137 416
MsaG007765 MtrunA17_Chr2g0296231 72.250 400 107 2 1 400 1 396 0.0 581
MsaG007765 MtrunA17_Chr2g0296171 74.873 394 84 3 1 384 7 395 0.0 570
MsaG007765 MtrunA17_Chr2g0296711 69.825 401 115 2 1 397 1 399 0.0 545
MsaG007765 MtrunA17_Chr2g0296181 69.598 398 94 2 1 398 1 371 0.0 541
MsaG007765 MtrunA17_Chr2g0296721 68.238 403 118 4 1 399 1 397 0.0 531
MsaG007765 MtrunA17_Chr2g0296741 66.501 403 126 3 1 399 21 418 0.0 525
MsaG007765 MtrunA17_Chr2g0315731 67.494 403 122 3 1 399 21 418 0.0 522
MsaG007765 MtrunA17_Chr2g0296291 68.939 396 84 3 36 396 19 410 0.0 516
MsaG007765 MtrunA17_Chr2g0296251 62.148 391 105 3 35 384 10 398 1.84e-158 451
MsaG007765 MtrunA17_Chr2g0296151 52.542 354 165 1 43 396 32 382 5.80e-129 376
MsaG007765 MtrunA17_Chr2g0295741 50.798 376 181 2 21 396 17 388 8.08e-127 371
MsaG007765 MtrunA17_Chr2g0296111 54.830 352 153 3 43 392 31 378 5.78e-126 368
MsaG007765 MtrunA17_Chr2g0295731 48.525 373 176 4 16 388 25 381 1.63e-122 359
MsaG007765 MtrunA17_Chr2g0296121 51.429 350 167 1 43 392 118 464 5.71e-121 358
MsaG007765 MtrunA17_Chr2g0296301 51.275 353 164 5 39 388 23 370 7.76e-117 345
MsaG007765 MtrunA17_Chr2g0296551 80.402 199 39 0 201 399 1 199 3.53e-113 329
MsaG007765 MtrunA17_Chr4g0067121 47.414 348 177 3 46 392 46 388 4.77e-112 333
MsaG007765 MtrunA17_Chr4g0067141 48.961 337 167 2 46 382 39 370 1.73e-101 306
MsaG007765 MtrunA17_Chr4g0067671 48.607 323 161 2 45 367 38 355 9.65e-99 298
MsaG007765 MtrunA17_Chr2g0295761 47.368 342 174 5 45 385 54 390 2.39e-98 298
MsaG007765 MtrunA17_Chr2g0296211 70.588 187 55 0 214 400 188 374 9.01e-91 278
MsaG007765 MtrunA17_Chr2g0296211 63.087 149 44 3 1 145 66 207 1.08e-48 169
MsaG007765 MtrunA17_Chr2g0296701 73.529 170 45 0 230 399 2 171 1.42e-85 258
MsaG007765 MtrunA17_Chr2g0286191 43.149 343 176 7 49 384 29 359 2.15e-81 254
MsaG007765 MtrunA17_Chr2g0296751 73.611 144 37 1 256 399 1 143 1.16e-69 217
MsaG007765 MtrunA17_Chr2g0296161 53.801 171 79 0 227 397 1 171 5.49e-62 197
MsaG007765 MtrunA17_Chr2g0296131 57.229 166 71 0 231 396 9 174 6.52e-62 197
MsaG007765 MtrunA17_Chr4g0001831 30.618 356 241 4 30 382 16 368 2.04e-47 166
MsaG007765 MtrunA17_Chr2g0295711 47.024 168 74 2 43 210 38 190 4.53e-43 149
MsaG007765 MtrunA17_Chr2g0286221 47.561 164 82 3 229 391 16 176 1.21e-41 145
MsaG007765 MtrunA17_Chr2g0295721 59.292 113 46 0 227 339 1 113 2.25e-40 139
MsaG007765 MtrunA17_Chr2g0295751 49.673 153 74 1 27 179 26 175 2.25e-38 144
MsaG007765 MtrunA17_Chr8g0377801 29.605 304 210 2 81 382 84 385 2.28e-35 134
MsaG007765 MtrunA17_Chr2g0296561 46.988 166 72 5 2 167 1 150 4.55e-33 121
MsaG007765 MtrunA17_Chr8g0377781 29.866 298 205 2 83 378 73 368 1.80e-30 120
MsaG007765 MtrunA17_Chr4g0067131 50.617 81 40 0 300 380 1 81 6.21e-22 89.4
MsaG007765 MtrunA17_Chr4g0067681 52.459 61 29 0 289 349 1 61 3.21e-15 70.1
MsaG007765 MtrunA17_Chr2g0294671 24.345 267 178 7 90 345 215 468 1.01e-11 66.2
MsaG007765 MtrunA17_Chr8g0339041 21.581 329 242 9 81 398 47 370 6.26e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007765 AT5G07900.1 32.537 335 215 4 53 381 70 399 6.16e-57 192
MsaG007765 AT1G21150.1 33.030 330 216 3 53 379 57 384 1.46e-51 177
MsaG007765 AT1G21150.3 32.335 334 221 3 49 379 17 348 4.51e-51 175
MsaG007765 AT1G21150.2 33.030 330 216 3 53 379 121 448 6.37e-51 177
MsaG007765 AT1G61990.2 29.775 356 197 8 53 368 53 395 1.04e-36 138
MsaG007765 AT1G61990.1 29.775 356 197 8 53 368 53 395 1.04e-36 138
MsaG007765 AT1G61980.3 28.249 354 208 8 53 367 53 399 1.95e-36 137
MsaG007765 AT1G61980.2 28.249 354 208 8 53 367 53 399 1.95e-36 137
MsaG007765 AT1G61980.1 28.249 354 208 8 53 367 53 399 1.95e-36 137
MsaG007765 AT1G61970.2 28.169 355 207 9 53 367 53 399 2.07e-35 135
MsaG007765 AT1G61970.7 28.169 355 207 9 53 367 53 399 2.07e-35 135
MsaG007765 AT1G61970.6 28.169 355 207 9 53 367 53 399 2.07e-35 135
MsaG007765 AT1G61970.3 28.169 355 207 9 53 367 53 399 2.07e-35 135
MsaG007765 AT1G61970.5 28.169 355 207 9 53 367 53 399 2.07e-35 135
MsaG007765 AT1G61970.4 28.169 355 207 9 53 367 53 399 2.07e-35 135
MsaG007765 AT1G61970.1 28.169 355 207 9 53 367 53 399 2.07e-35 135
MsaG007765 AT1G62120.1 27.966 354 215 7 51 367 55 405 8.99e-33 128
MsaG007765 AT5G64950.1 26.331 357 233 7 32 373 25 366 1.82e-28 115
MsaG007765 AT3G46950.1 29.520 271 176 6 35 299 30 291 3.03e-26 110
MsaG007765 AT1G62085.1 27.855 359 239 10 53 400 56 405 1.21e-23 102
MsaG007765 AT1G62085.2 27.855 359 239 10 53 400 56 405 1.21e-23 102
MsaG007765 AT1G62010.1 26.780 295 173 5 87 344 71 359 3.03e-23 100
MsaG007765 AT1G62150.1 26.593 361 241 8 53 400 59 408 1.07e-22 100
MsaG007765 AT1G61960.1 27.941 272 191 3 26 296 26 293 1.50e-22 99.4
MsaG007765 AT1G62110.1 28.629 248 167 4 53 296 53 294 1.01e-21 97.1
MsaG007765 AT5G23930.1 27.509 269 176 5 27 286 22 280 2.65e-18 87.0

Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTTCATTGGCTACTTT+TGG 0.140203 2:+22665221 MsaT007765.1:CDS
GTCAATTTGATGTCTGATTT+TGG 0.159011 2:+22665095 MsaT007765.1:CDS
TCATACCTCATCAACAAATT+TGG 0.185260 2:+22664669 MsaT007765.1:CDS
AAGCTAATGTGTAAAATTCT+AGG 0.238524 2:-22664942 None:intergenic
ATAACTGAATCAGGCTTATC+TGG 0.241119 2:-22664750 None:intergenic
AAAAGAGGGTTGTTGAGTTT+TGG 0.250282 2:-22664570 None:intergenic
TTGGAGGAAGTTAAGTGTTT+AGG 0.257551 2:+22665194 MsaT007765.1:CDS
AAGTCAAGGCCATCTATATT+TGG 0.261665 2:+22665149 MsaT007765.1:CDS
TGGAGGAAGTTAAGTGTTTA+GGG 0.262883 2:+22665195 MsaT007765.1:CDS
AAACTACCAATGACATTATT+AGG 0.279319 2:-22665122 None:intergenic
TGGCATACCATTCACTAATT+AGG 0.304191 2:+22665685 MsaT007765.1:CDS
ATGCCATTCTTTGTTTAATT+CGG 0.306732 2:+22665024 MsaT007765.1:CDS
AGACCAGCCCCATTTCTTAA+AGG 0.307294 2:-22665303 None:intergenic
TGATTCCTAAAACAACATTC+TGG 0.312730 2:-22664527 None:intergenic
AAAGGATTTGATTAAGTCAT+TGG 0.323129 2:+22665175 MsaT007765.1:CDS
ATTGAAACCAACATCAAATT+AGG 0.361121 2:-22665359 None:intergenic
AAGTTGATGCCTTTAAGAAA+TGG 0.364427 2:+22665294 MsaT007765.1:CDS
TTTGATGATGATGCGTATAT+CGG 0.373047 2:-22664805 None:intergenic
AGAAGCTTCTGAGTGAAATT+AGG 0.377101 2:-22664611 None:intergenic
AAGAAGGTGATAACTGAATC+AGG 0.385522 2:-22664759 None:intergenic
AGTTGATGCCTTTAAGAAAT+GGG 0.392127 2:+22665295 MsaT007765.1:CDS
TCCTTTGAACCAAATATAGA+TGG 0.398078 2:-22665158 None:intergenic
ATCAGATGCAGAAGCACCTT+TGG 0.398697 2:-22664895 None:intergenic
ACAAAGAATGGCATCCTTGT+TGG 0.401490 2:-22665015 None:intergenic
TTCAGCTTTAAGAAGGAGTC+TGG 0.405388 2:+22665605 MsaT007765.1:CDS
GGAGTTCCTAATAATGTCAT+TGG 0.406851 2:+22665116 MsaT007765.1:CDS
TTTGTTATAGTTTGGAGAAA+AGG 0.408694 2:+22665465 MsaT007765.1:CDS
TTTAGGGTTTCATCCTTCAT+TGG 0.414145 2:+22665211 MsaT007765.1:CDS
GCCATCTATATTTGGTTCAA+AGG 0.427799 2:+22665157 MsaT007765.1:CDS
AGAAAATGGTGCAGCTAAGC+TGG 0.430292 2:-22665549 None:intergenic
CTTTGGCAAAATAACATTGT+GGG 0.433749 2:-22664856 None:intergenic
TCAATTGGGTTGGAATTCCT+TGG 0.440659 2:+22665418 MsaT007765.1:CDS
GAGAAGTGTTGGTTCAAAAG+AGG 0.453001 2:-22664585 None:intergenic
GAAATTACCTAATTAGTGAA+TGG 0.466047 2:-22665692 None:intergenic
GATTCCTAAAACAACATTCT+GGG 0.469695 2:-22664526 None:intergenic
ATTATCCACAGTAAGGTGAT+AGG 0.476240 2:-22665072 None:intergenic
AGCTAATGTGTAAAATTCTA+GGG 0.492180 2:-22664941 None:intergenic
ATGCCGAATTAAACAAAGAA+TGG 0.493192 2:-22665027 None:intergenic
ATGAGGTATGATACAGCAAA+TGG 0.505053 2:-22664657 None:intergenic
TTTGGGTCAATCAATTGGGT+TGG 0.506924 2:+22665408 MsaT007765.1:CDS
GTTGCAGCCTAATTTGATGT+TGG 0.507199 2:+22665352 MsaT007765.1:CDS
AAATTAGGAACGAGAAGTGT+TGG 0.518663 2:-22664596 None:intergenic
TAACTGAATCAGGCTTATCT+GGG 0.520618 2:-22664749 None:intergenic
CAAAGCTTTCAACCTCAAGC+AGG 0.538715 2:+22664713 MsaT007765.1:CDS
ACCCCAAAAGTAGCCAATGA+AGG 0.540625 2:-22665224 None:intergenic
AATTGCATAACTGAGGCCCT+TGG 0.542717 2:-22665494 None:intergenic
AGAAGTGTTGGTTCAAAAGA+GGG 0.551032 2:-22664584 None:intergenic
ATGCCTTTAAGAAATGGGGC+TGG 0.559676 2:+22665300 MsaT007765.1:CDS
AATTGACATTATCCACAGTA+AGG 0.560808 2:-22665079 None:intergenic
TTGGTAGTTTGCTTAAGTCA+AGG 0.561949 2:+22665135 MsaT007765.1:CDS
ACTTTGGCAAAATAACATTG+TGG 0.562030 2:-22664857 None:intergenic
TGGAGAAAAGGATCATTCCA+AGG 0.570897 2:+22665477 MsaT007765.1:CDS
GGATTTGATTAAGTCATTGG+AGG 0.571043 2:+22665178 MsaT007765.1:CDS
TGAAACCAAATTTGTTGATG+AGG 0.579354 2:-22664674 None:intergenic
TTTGGCAAAATAACATTGTG+GGG 0.586854 2:-22664855 None:intergenic
AAGAAACAGTTTCTTCAACA+TGG 0.593202 2:-22664502 None:intergenic
TATCGGAGTGTGAAAAGCCA+TGG 0.608796 2:-22664788 None:intergenic
AATGAAACTTGCATACACAA+TGG 0.609213 2:+22665655 MsaT007765.1:CDS
TATTGAAACGAACCTGCTTG+AGG 0.609752 2:-22664725 None:intergenic
ACTCAGAAGCTTCTGCACAT+CGG 0.615740 2:+22664620 MsaT007765.1:CDS
GTTGATGCCTTTAAGAAATG+GGG 0.618010 2:+22665296 MsaT007765.1:CDS
TGGTAGTTTGATAAGTGCCA+AGG 0.620470 2:-22665435 None:intergenic
AAACTATAACAAAACATCTG+TGG 0.633270 2:-22665455 None:intergenic
CAAAAGAAATTGCATAACTG+AGG 0.634260 2:-22665501 None:intergenic
TGAGGTATGATACAGCAAAT+GGG 0.638616 2:-22664656 None:intergenic
GGAGAAAAGGATCATTCCAA+GGG 0.639024 2:+22665478 MsaT007765.1:CDS
GCATACACAATGGAAAACAA+TGG 0.644592 2:+22665665 MsaT007765.1:CDS
AACTGAATCAGGCTTATCTG+GGG 0.674780 2:-22664748 None:intergenic
GAATTCCTATCACCTTACTG+TGG 0.683150 2:+22665067 MsaT007765.1:CDS
GGTTGTGATGAAAGTAACGA+AGG 0.709568 2:-22664834 None:intergenic
AAAGCTTTGAGAGCTGACTG+AGG 0.710331 2:-22664699 None:intergenic
TAGGTTTATGAAAACCAACA+AGG 0.716946 2:+22665001 MsaT007765.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22664504 22665706 22664504 ID=MsaG007765
Chr2 mRNA 22664504 22665706 22664504 ID=MsaT007765.1;Parent=MsaG007765
Chr2 exon 22664504 22665706 22664504 ID=MsaT007765.1.exon1;Parent=MsaT007765.1
Chr2 CDS 22664504 22665706 22664504 ID=cds.MsaT007765.1;Parent=MsaT007765.1
Gene Sequence

>MsaT007765.1

ATGTTGAAGAAACTGTTTCTTTCCCAGAATGTTGTTTTAGGAATCATCACAGCAAAATCAACAACCCCAAAACTCAACAACCCTCTTTTGAACCAACACTTCTCGTTCCTAATTTCACTCAGAAGCTTCTGCACATCGGAATCAGACACACACCCATTTGCTGTATCATACCTCATCAACAAATTTGGTTTCACACCTCAGTCAGCTCTCAAAGCTTTCAACCTCAAGCAGGTTCGTTTCAATACCCCAGATAAGCCTGATTCAGTTATCACCTTCTTCAAAAACCATGGCTTTTCACACTCCGATATACGCATCATCATCAAAAAAACACCTTCGTTACTTTCATCACAACCCCACAATGTTATTTTGCCAAAGTTTCAATTTTTTCTATCCAAAGGTGCTTCTGCATCTGATATTATTTCATTGTTAACTGAAAACCCTAGAATTTTACACATTAGCTTGGAGAAACGAATAATCCCTCTTTTTGAATTGTTAAGTAGGTTTATGAAAACCAACAAGGATGCCATTCTTTGTTTAATTCGGCATTCGACTGCATTTTCTATGAATTCCTATCACCTTACTGTGGATAATGTCAATTTGATGTCTGATTTTGGAGTTCCTAATAATGTCATTGGTAGTTTGCTTAAGTCAAGGCCATCTATATTTGGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGTGTTTAGGGTTTCATCCTTCATTGGCTACTTTTGGGGTTGCTTTGAAAGCCAAGAAATGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATGCCTTTAAGAAATGGGGCTGGTCTGATGAAGCTGTTATTCGAACATTTCAGTTGCAGCCTAATTTGATGTTGGTTTCAATTGATAAGATTGATTTGTTGATGAGTTTTTGGGTCAATCAATTGGGTTGGAATTCCTTGGCACTTATCAAACTACCACAGATGTTTTGTTATAGTTTGGAGAAAAGGATCATTCCAAGGGCCTCAGTTATGCAATTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCCAGCTTAGCTGCACCATTTTCTTATTCTGAGAAAATATTTTTGAGCAAGTTTGTTTTCAGCTTTAAGAAGGAGTCTGGTTATCTATTAAAGCTATATGAGCAAAAAATGAAACTTGCATACACAATGGAAAACAATGGCATACCATTCACTAATTAG
Protein sequence

>MsaT007765.1

MLKKLFLSQNVVLGIITAKSTTPKLNNPLLNQHFSFLISLRSFCTSESDTHPFAVSYLINKFGFTPQSALKAFNLKQVRFNTPDKPDSVITFFKNHGFSHSDIRIIIKKTPSLLSSQPHNVILPKFQFFLSKGASASDIISLLTENPRILHISLEKRIIPLFELLSRFMKTNKDAILCLIRHSTAFSMNSYHLTVDNVNLMSDFGVPNNVIGSLLKSRPSIFGSKDLIKSLEEVKCLGFHPSLATFGVALKAKKCLSKKLWDEKVDAFKKWGWSDEAVIRTFQLQPNLMLVSIDKIDLLMSFWVNQLGWNSLALIKLPQMFCYSLEKRIIPRASVMQFLLMKGLRKKNASLAAPFSYSEKIFLSKFVFSFKKESGYLLKLYEQKMKLAYTMENNGIPFTN*