AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007768


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007768 MtrunA17_Chr2g0296191 93.680 269 17 0 28 296 242 510 2.68e-176 515
MsaG007768 MtrunA17_Chr2g0296191 60.000 290 105 3 18 296 708 997 6.10e-111 345
MsaG007768 MtrunA17_Chr2g0296251 63.487 304 97 4 3 295 97 397 3.31e-128 371
MsaG007768 MtrunA17_Chr2g0296221 61.386 303 106 3 18 309 125 427 6.01e-127 369
MsaG007768 MtrunA17_Chr2g0296721 60.396 303 112 2 18 312 96 398 5.69e-126 365
MsaG007768 MtrunA17_Chr2g0315731 64.184 282 98 1 34 312 138 419 6.05e-125 363
MsaG007768 MtrunA17_Chr2g0296711 57.895 304 119 2 18 312 99 402 5.39e-124 362
MsaG007768 MtrunA17_Chr2g0296741 57.190 306 122 2 16 312 114 419 3.43e-122 357
MsaG007768 MtrunA17_Chr2g0296291 56.347 323 103 2 28 312 92 414 1.46e-118 347
MsaG007768 MtrunA17_Chr2g0296231 56.623 302 120 3 19 311 96 395 1.02e-116 342
MsaG007768 MtrunA17_Chr2g0296171 57.093 289 113 3 18 295 106 394 3.26e-109 323
MsaG007768 MtrunA17_Chr2g0296181 55.298 302 99 4 18 310 97 371 1.72e-103 308
MsaG007768 MtrunA17_Chr2g0296151 49.831 295 142 1 16 304 84 378 9.12e-102 303
MsaG007768 MtrunA17_Chr2g0295741 50.167 299 143 1 16 308 90 388 3.80e-99 297
MsaG007768 MtrunA17_Chr2g0296111 51.178 297 136 3 16 304 83 378 1.05e-94 285
MsaG007768 MtrunA17_Chr2g0296121 48.148 297 148 1 18 308 172 468 1.18e-92 283
MsaG007768 MtrunA17_Chr2g0296551 66.332 199 67 0 113 311 1 199 1.33e-92 273
MsaG007768 MtrunA17_Chr4g0067121 51.799 278 134 0 27 304 111 388 1.35e-91 278
MsaG007768 MtrunA17_Chr2g0296211 68.817 186 58 0 125 310 187 372 6.31e-89 270
MsaG007768 MtrunA17_Chr2g0296301 44.521 292 155 2 28 312 96 387 9.30e-89 270
MsaG007768 MtrunA17_Chr2g0295731 45.695 302 136 4 16 300 91 381 1.98e-88 269
MsaG007768 MtrunA17_Chr2g0296701 71.930 171 48 0 142 312 2 172 3.12e-85 254
MsaG007768 MtrunA17_Chr2g0295761 48.110 291 144 2 16 299 102 392 3.74e-84 258
MsaG007768 MtrunA17_Chr4g0067141 44.788 259 143 0 36 294 112 370 3.46e-70 222
MsaG007768 MtrunA17_Chr4g0067671 41.852 270 151 1 16 279 86 355 4.94e-67 213
MsaG007768 MtrunA17_Chr2g0286191 42.361 288 156 4 16 295 73 358 8.62e-66 211
MsaG007768 MtrunA17_Chr2g0296751 66.897 145 47 1 168 312 1 144 3.58e-64 200
MsaG007768 MtrunA17_Chr2g0296131 56.627 166 72 0 143 308 9 174 1.03e-63 199
MsaG007768 MtrunA17_Chr2g0296161 50.588 170 84 0 140 309 2 171 1.49e-58 186
MsaG007768 MtrunA17_Chr2g0295721 60.360 111 44 0 140 250 2 112 1.97e-43 145
MsaG007768 MtrunA17_Chr2g0286221 43.558 163 90 2 139 301 14 174 1.74e-38 134
MsaG007768 MtrunA17_Chr4g0001831 30.717 293 186 7 16 294 79 368 3.20e-30 117
MsaG007768 MtrunA17_Chr8g0377801 25.993 277 200 3 21 294 111 385 2.77e-24 101
MsaG007768 MtrunA17_Chr8g0377781 27.099 262 188 2 32 290 107 368 3.67e-22 95.5
MsaG007768 MtrunA17_Chr4g0067131 45.679 81 44 0 212 292 1 81 9.45e-19 79.3
MsaG007768 MtrunA17_Chr2g0295711 37.963 108 57 3 16 115 90 195 6.20e-13 66.6
MsaG007768 MtrunA17_Chr4g0067681 47.541 61 32 0 201 261 1 61 9.24e-13 62.4
MsaG007768 MtrunA17_Chr2g0295751 42.683 82 41 1 16 91 91 172 9.52e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007768 AT5G07900.1 32.342 269 174 4 32 293 132 399 3.48e-41 148
MsaG007768 AT1G21150.3 28.727 275 190 3 28 296 79 353 7.35e-33 124
MsaG007768 AT1G21150.1 28.727 275 190 3 28 296 115 389 1.66e-32 124
MsaG007768 AT1G21150.2 28.727 275 190 3 28 296 179 453 3.21e-32 124
MsaG007768 AT1G61970.2 29.808 312 166 9 16 279 93 399 3.05e-23 99.4
MsaG007768 AT1G61970.7 29.808 312 166 9 16 279 93 399 3.05e-23 99.4
MsaG007768 AT1G61970.6 29.808 312 166 9 16 279 93 399 3.05e-23 99.4
MsaG007768 AT1G61970.3 29.808 312 166 9 16 279 93 399 3.05e-23 99.4
MsaG007768 AT1G61970.5 29.808 312 166 9 16 279 93 399 3.05e-23 99.4
MsaG007768 AT1G61970.4 29.808 312 166 9 16 279 93 399 3.05e-23 99.4
MsaG007768 AT1G61970.1 29.808 312 166 9 16 279 93 399 3.05e-23 99.4
MsaG007768 AT5G64950.1 28.571 273 173 6 39 294 109 376 9.15e-23 97.8
MsaG007768 AT1G61980.3 28.155 309 175 7 16 279 93 399 1.24e-22 97.8
MsaG007768 AT1G61980.2 28.155 309 175 7 16 279 93 399 1.24e-22 97.8
MsaG007768 AT1G61980.1 28.155 309 175 7 16 279 93 399 1.24e-22 97.8
MsaG007768 AT1G61990.2 26.074 326 189 7 16 296 93 411 1.82e-21 94.4
MsaG007768 AT1G61990.1 26.074 326 189 7 16 296 93 411 1.82e-21 94.4
MsaG007768 AT1G62010.1 26.299 308 180 6 16 279 79 383 1.99e-20 91.3
MsaG007768 AT3G46950.1 24.691 324 162 9 32 280 115 431 9.92e-18 83.6
MsaG007768 AT1G62120.1 26.490 302 167 8 23 279 114 405 1.90e-17 82.8
MsaG007768 AT1G62110.1 28.033 239 154 6 16 239 93 328 9.59e-16 77.8
MsaG007768 AT1G61960.1 28.846 208 128 5 16 209 93 294 6.18e-15 75.5
MsaG007768 AT1G62085.1 31.579 209 120 7 16 208 96 297 1.89e-14 73.9
MsaG007768 AT1G62085.2 31.579 209 120 7 16 208 96 297 1.89e-14 73.9
MsaG007768 AT1G62150.1 28.571 217 130 6 7 208 94 300 4.20e-13 70.1

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTATTCTGTAAAATTCT+AGG 0.190911 2:-22685989 None:intergenic
TTCAATTGATAAGATTAATT+TGG 0.211307 2:+22686414 MsaT007768.1:CDS
TTGGAGGAAGTTAAGGGTTT+AGG 0.213631 2:+22686232 MsaT007768.1:CDS
GACCCTTCAACTTCAACTTT+TGG 0.225478 2:+22686259 MsaT007768.1:CDS
TGGAGGAAGTTAAGGGTTTA+GGG 0.238470 2:+22686233 MsaT007768.1:CDS
ATTCTTCGGCACTTTCTAAA+AGG 0.266057 2:+22686470 MsaT007768.1:CDS
AGATTATGAAGTCACCTATT+TGG 0.280909 2:-22686737 None:intergenic
GTTAAGTTATTGATTGACTA+TGG 0.285651 2:+22686133 MsaT007768.1:CDS
ACCAGGGATTGAAACCCTTT+TGG 0.291942 2:-22685094 None:intergenic
TAGTACTTTGGAGGAAGTTA+AGG 0.340131 2:+22686225 MsaT007768.1:CDS
CCCAAAAGGGTTTCAATCCC+TGG 0.352735 2:+22685093 MsaT007768.1:CDS
TGACATGCCATTCACCAAAT+AGG 0.363432 2:+22686723 MsaT007768.1:CDS
TTAGTTACAGTTTGCAGAAA+AGG 0.377592 2:+22686503 MsaT007768.1:CDS
TGAACCGTTTGCTCCGTCTA+AGG 0.377750 2:+22686597 MsaT007768.1:CDS
CTTTGGAAGAATTGAATTGT+GGG 0.379663 2:-22685903 None:intergenic
GATGTTATTCGTGCATTTAG+AGG 0.388931 2:+22686370 MsaT007768.1:CDS
TTGACAAGACCAGCTATACT+TGG 0.394866 2:+22686187 MsaT007768.1:CDS
AATTGATTTCCTTAGTACTT+TGG 0.395237 2:+22686213 MsaT007768.1:CDS
TCAATTGGGTTGGAATTCTT+CGG 0.404558 2:+22686456 MsaT007768.1:CDS
AGTACTTTGGAGGAAGTTAA+GGG 0.430815 2:+22686226 MsaT007768.1:CDS
ATGTATGAGTAAAACACTTT+GGG 0.441511 2:+22686303 MsaT007768.1:CDS
GAAGTCACCTATTTGGTGAA+TGG 0.443693 2:-22686730 None:intergenic
TTCCTCAAACCTTTCATCAC+AGG 0.451862 2:-22686556 None:intergenic
AATGTATGAGTAAAACACTT+TGG 0.452530 2:+22686302 MsaT007768.1:CDS
TTCCTGTGATGAAAGGTTTG+AGG 0.465337 2:+22686554 MsaT007768.1:CDS
AACTGAGAACATATTTGCTT+AGG 0.466357 2:-22686627 None:intergenic
TTATTTATTAAAGCTTTATG+AGG 0.468070 2:+22686666 MsaT007768.1:CDS
TTAACAGTAGTAAGATTGTA+AGG 0.474106 2:-22686109 None:intergenic
CTGCAATTTCCTGTGATGAA+AGG 0.481186 2:+22686547 MsaT007768.1:CDS
AAGGATTGTTCCAAGGGCAT+TGG 0.488885 2:+22686522 MsaT007768.1:CDS
TCAATTGATCCAAGTATAGC+TGG 0.498779 2:-22686196 None:intergenic
TGATTTCCTTAGTACTTTGG+AGG 0.499163 2:+22686216 MsaT007768.1:CDS
CCACAAGGATCATAAGTTGA+TGG 0.500141 2:-22685148 None:intergenic
CTTAGGAACATTTCCTTAGA+CGG 0.503257 2:-22686610 None:intergenic
ACGGCCAATGAAAACCCAAA+AGG 0.504614 2:+22685079 None:intergenic
CGGCCAATGAAAACCCAAAA+GGG 0.509267 2:+22685080 None:intergenic
ATTGAAGTCAACAACAAGTC+AGG 0.516236 2:-22686397 None:intergenic
TAACTTCCTCCAAAGTACTA+AGG 0.517307 2:-22686222 None:intergenic
ACTTTGGAAGAATTGAATTG+TGG 0.522321 2:-22685904 None:intergenic
ATTTCCTTAGACGGAGCAAA+CGG 0.526804 2:-22686601 None:intergenic
TTTGGGTTAATCAATTGGGT+TGG 0.527975 2:+22686446 MsaT007768.1:CDS
AGCAAACGGTTCAACTAAGC+TGG 0.533205 2:-22686587 None:intergenic
GACCCAAAAGTTGAAGTTGA+AGG 0.537017 2:-22686262 None:intergenic
AATTGCAGAACCAATGCCCT+TGG 0.540852 2:-22686532 None:intergenic
TGCAGAAAAGGATTGTTCCA+AGG 0.541911 2:+22686515 MsaT007768.1:CDS
GCAGAAAAGGATTGTTCCAA+GGG 0.547363 2:+22686516 MsaT007768.1:CDS
AGAGAATTCATGTGAAAACC+AGG 0.560693 2:-22685111 None:intergenic
ACCCAAAAGTTGAAGTTGAA+GGG 0.582729 2:-22686261 None:intergenic
CAAAAGCAATCTAGCAATAG+CGG 0.586332 2:-22686167 None:intergenic
TCAACTTATGATCCTTGTGG+TGG 0.587243 2:+22685151 MsaT007768.1:CDS
ATGATCCTTGTGGTGGATTG+CGG 0.607856 2:+22685158 MsaT007768.1:CDS
CCATCAACTTATGATCCTTG+TGG 0.634672 2:+22685148 MsaT007768.1:CDS
CATGTCAATGTTCTCCTCAG+TGG 0.650724 2:-22686707 None:intergenic
GAGAATTCATGTGAAAACCA+GGG 0.658766 2:-22685110 None:intergenic
CCTTACCGCAATCCACCACA+AGG 0.671980 2:-22685163 None:intergenic
CGTAAATCTTGCATCCACTG+AGG 0.719555 2:+22686693 MsaT007768.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22685086 22686744 22685086 ID=MsaG007768
Chr2 mRNA 22685086 22686744 22685086 ID=MsaT007768.1;Parent=MsaG007768
Chr2 exon 22685086 22685179 22685086 ID=MsaT007768.1.exon1;Parent=MsaT007768.1
Chr2 CDS 22685086 22685179 22685086 ID=cds.MsaT007768.1;Parent=MsaT007768.1
Chr2 exon 22685900 22686744 22685900 ID=MsaT007768.1.exon2;Parent=MsaT007768.1
Chr2 CDS 22685900 22686744 22685900 ID=cds.MsaT007768.1;Parent=MsaT007768.1
Gene Sequence

>MsaT007768.1

ATGAAAACCCAAAAGGGTTTCAATCCCTGGTTTTCACATGAATTCTCTTTTGGATATGCTCACCATCAACTTATGATCCTTGTGGTGGATTGCGATACCCACAATTCAATTCTTCCAAAGTTTCAATTTTTTCTATCTAAAGGCGCTTCAGCTTCTGAAATCGCGTGTTTGTTAGCAGCGAATCCTAGAATTTTACAGAATAGTTTGGAGAATAGAATAATTCCTCTTTTTGAATTTTATAAGAGGTTTTTTAAATCTGATAAAGCTACAATTTGTTGCATCATTCGAAATTTGATATCTTTGCCTTACAATCTTACTACTGTTAATGTTAAGTTATTGATTGACTATGGAGTTTGTGATTCCGCTATTGCTAGATTGCTTTTGACAAGACCAGCTATACTTGGATCAATTGATTTCCTTAGTACTTTGGAGGAAGTTAAGGGTTTAGGGTTTGACCCTTCAACTTCAACTTTTGGGTCAGCTTTGGTTGCAAAGAAATGTATGAGTAAAACACTTTGGGATGAGAAAGTTGATGTTTTTAAGAAATGGGGTTGGTCTGATGAAGATGTTATTCGTGCATTTAGAGGTCGTCCTGACTTGTTGTTGACTTCAATTGATAAGATTAATTTGGTGTTGAGTTTTTGGGTTAATCAATTGGGTTGGAATTCTTCGGCACTTTCTAAAAGGCCACGTATTTTTAGTTACAGTTTGCAGAAAAGGATTGTTCCAAGGGCATTGGTTCTGCAATTTCCTGTGATGAAAGGTTTGAGGAAAAAGAATGCCAGCTTAGTTGAACCGTTTGCTCCGTCTAAGGAAATGTTCCTAAGCAAATATGTTCTCAGTTTTAAGGAGGATTCTGATTATTTATTAAAGCTTTATGAGGAAAACGTAAATCTTGCATCCACTGAGGAGAACATTGACATGCCATTCACCAAATAG

Protein sequence

>MsaT007768.1

MKTQKGFNPWFSHEFSFGYAHHQLMILVVDCDTHNSILPKFQFFLSKGASASEIACLLAANPRILQNSLENRIIPLFEFYKRFFKSDKATICCIIRNLISLPYNLTTVNVKLLIDYGVCDSAIARLLLTRPAILGSIDFLSTLEEVKGLGFDPSTSTFGSALVAKKCMSKTLWDEKVDVFKKWGWSDEDVIRAFRGRPDLLLTSIDKINLVLSFWVNQLGWNSSALSKRPRIFSYSLQKRIVPRALVLQFPVMKGLRKKNASLVEPFAPSKEMFLSKYVLSFKEDSDYLLKLYEENVNLASTEENIDMPFTK*