AlfalfaGEDB | MsaG007769

M. sativa ssp. caerulea / MsaG007769

KEGG: K15032
GO: -
PFAM: PF02536.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007769	KEH37295.1	67.347	392	75	3	15	355	23	412	1.35e-179	514

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007769	tr\|A0A072VGL0\|A0A072VGL0_MEDTR	67.347	392	75	3	15	355	23	412	6.43e-180	514

TFs/TRs:

Gene ID	Type	Classification
MsaG007769	TR	mTERF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001295	MsaG007769	0.801852	1.242000e-48	1.751122e-46
MsaG004030	MsaG007769	0.801630	1.378871e-48	1.934282e-46
MsaG005387	MsaG007769	0.820172	1.382549e-52	3.054770e-50
MsaG006833	MsaG007769	0.804893	2.932594e-49	4.434942e-47
MsaG006934	MsaG007769	0.801338	1.581937e-48	2.204493e-46
MsaG007769	MsaG012586	0.813951	3.404787e-51	6.410604e-49
MsaG007769	MsaG015953	0.813140	5.124652e-51	9.454322e-49
MsaG007769	MsaG018015	0.808472	5.186781e-50	8.537302e-48
MsaG007769	MsaG023630	0.810247	2.168073e-50	3.724779e-48
MsaG007769	MsaG024879	0.800959	1.888703e-48	2.609476e-46
MsaG007769	MsaG026891	0.806864	1.134768e-49	1.797294e-47
MsaG007769	MsaG027395	0.801984	1.167397e-48	1.650847e-46
MsaG007769	MsaG029989	0.811307	1.281692e-50	2.259789e-48
MsaG007769	MsaG031064	0.809946	2.515221e-50	4.289618e-48
MsaG007769	MsaG032773	0.804188	4.106646e-49	6.109914e-47
MsaG007769	MsaG035659	0.801500	1.465926e-48	2.050296e-46
MsaG007769	MsaG041920	0.803200	6.570493e-49	9.554454e-47
MsaG007769	MsaG044049	0.848007	1.524747e-59	7.723966e-57
MsaG007769	MsaG045593	0.813562	4.144670e-51	7.727704e-49
MsaG007769	MsaG046023	0.806437	1.394973e-49	2.187451e-47
MsaG007769	MsaG046357	0.823424	2.465837e-53	5.942334e-51
MsaG007769	MsaG046537	0.827687	2.435753e-54	6.602526e-52
MsaG007769	MsaG001966	0.808103	6.212234e-50	1.013459e-47
MsaG007769	MsaG002662	0.816928	7.464393e-52	1.515568e-49
MsaG007769	MsaG009163	0.823498	2.369859e-53	5.722441e-51
MsaG007769	MsaG020114	0.804138	4.206711e-49	6.251440e-47
MsaG007769	MsaG028203	0.814208	2.990199e-51	5.666308e-49
MsaG007769	MsaG030294	0.801361	1.564959e-48	2.181919e-46
MsaG007769	MsaG036928	0.835552	2.865036e-56	9.756896e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007769	MtrunA17_Chr2g0296221	67.347	392	75	3	15	355	47	436	0.0	513
MsaG007769	MtrunA17_Chr2g0296711	60.934	407	100	5	1	352	1	403	1.05e-155	444
MsaG007769	MtrunA17_Chr2g0296171	59.383	389	102	4	1	333	7	395	1.13e-148	425
MsaG007769	MtrunA17_Chr2g0296191	63.215	367	82	3	15	330	630	994	1.42e-148	445
MsaG007769	MtrunA17_Chr2g0296191	51.813	386	120	7	1	332	136	509	1.57e-108	340
MsaG007769	MtrunA17_Chr2g0296231	57.816	403	103	6	1	346	1	393	5.07e-147	421
MsaG007769	MtrunA17_Chr2g0296741	58.201	378	108	3	22	353	48	421	3.99e-145	417
MsaG007769	MtrunA17_Chr2g0296211	61.345	357	82	4	1	349	66	374	2.30e-139	400
MsaG007769	MtrunA17_Chr2g0296721	56.992	379	112	4	22	354	28	401	1.55e-136	394
MsaG007769	MtrunA17_Chr2g0315731	56.878	378	113	3	22	353	48	421	3.26e-135	392
MsaG007769	MtrunA17_Chr2g0296181	53.980	402	95	6	1	345	1	369	1.02e-128	374
MsaG007769	MtrunA17_Chr2g0296291	66.065	277	82	2	87	353	142	416	1.40e-118	349
MsaG007769	MtrunA17_Chr2g0296291	38.854	157	71	7	24	168	18	161	4.14e-15	75.9
MsaG007769	MtrunA17_Chr2g0296251	64.982	277	77	2	67	333	132	398	1.26e-113	336
MsaG007769	MtrunA17_Chr2g0296251	42.138	159	65	5	30	173	20	166	3.25e-21	94.0
MsaG007769	MtrunA17_Chr2g0296551	77.157	197	45	0	150	346	1	197	3.64e-105	307
MsaG007769	MtrunA17_Chr2g0296111	45.293	393	148	6	1	341	1	378	9.32e-96	290
MsaG007769	MtrunA17_Chr2g0295741	43.622	392	154	6	6	345	12	388	5.08e-94	285
MsaG007769	MtrunA17_Chr2g0296151	43.514	370	150	4	26	345	22	382	4.54e-93	283
MsaG007769	MtrunA17_Chr2g0296121	45.170	352	144	2	40	345	120	468	1.78e-91	281
MsaG007769	MtrunA17_Chr2g0295731	42.733	344	148	2	40	337	41	381	9.16e-85	261
MsaG007769	MtrunA17_Chr2g0296301	40.390	359	157	5	30	340	24	373	2.10e-81	253
MsaG007769	MtrunA17_Chr4g0067121	39.943	348	157	4	41	341	46	388	9.09e-80	249
MsaG007769	MtrunA17_Chr2g0296701	69.714	175	51	1	179	353	2	174	1.46e-78	238
MsaG007769	MtrunA17_Chr4g0067671	40.741	324	147	4	36	316	34	355	1.37e-71	227
MsaG007769	MtrunA17_Chr2g0295761	42.229	341	149	7	39	334	53	390	6.67e-70	223
MsaG007769	MtrunA17_Chr4g0067141	39.233	339	155	4	38	330	36	369	9.21e-69	220
MsaG007769	MtrunA17_Chr2g0296751	67.785	149	45	1	205	353	1	146	4.80e-63	198
MsaG007769	MtrunA17_Chr2g0296131	55.422	166	74	0	180	345	9	174	1.16e-58	187
MsaG007769	MtrunA17_Chr2g0296161	51.462	171	83	0	176	346	1	171	2.52e-58	186
MsaG007769	MtrunA17_Chr2g0286191	36.842	342	149	8	46	333	31	359	1.04e-53	181
MsaG007769	MtrunA17_Chr2g0295721	61.062	113	44	0	176	288	1	113	4.67e-40	137
MsaG007769	MtrunA17_Chr2g0286221	46.795	156	81	2	178	333	16	169	7.55e-38	134
MsaG007769	MtrunA17_Chr4g0001831	25.291	344	207	5	35	330	26	367	3.46e-28	113
MsaG007769	MtrunA17_Chr2g0296561	55.128	78	29	2	37	114	31	102	5.81e-18	80.1
MsaG007769	MtrunA17_Chr4g0067131	45.679	81	44	0	249	329	1	81	4.72e-17	75.5
MsaG007769	MtrunA17_Chr2g0295711	50.000	76	38	0	40	115	40	115	2.46e-16	77.0
MsaG007769	MtrunA17_Chr2g0295751	51.948	77	37	0	40	116	41	117	2.68e-15	77.0
MsaG007769	MtrunA17_Chr8g0377781	25.882	255	175	4	85	327	116	368	4.69e-15	75.5
MsaG007769	MtrunA17_Chr8g0377801	23.077	312	224	5	33	330	75	384	6.23e-15	75.5
MsaG007769	MtrunA17_Chr4g0067681	50.820	61	30	0	238	298	1	61	3.11e-12	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007769	AT5G07900.1	28.686	373	202	9	16	330	33	399	1.58e-37	139
MsaG007769	AT1G21150.1	26.061	330	193	5	48	328	57	384	2.95e-28	114
MsaG007769	AT1G21150.3	25.749	334	197	5	44	328	17	348	2.95e-28	113
MsaG007769	AT1G21150.2	26.061	330	193	5	48	328	121	448	9.08e-28	113
MsaG007769	AT5G64950.1	26.513	347	198	8	39	330	31	375	1.61e-21	95.1
MsaG007769	AT1G61980.3	24.857	350	179	11	48	316	53	399	8.37e-15	75.5
MsaG007769	AT1G61980.2	24.857	350	179	11	48	316	53	399	8.37e-15	75.5
MsaG007769	AT1G61980.1	24.857	350	179	11	48	316	53	399	8.37e-15	75.5
MsaG007769	AT1G61970.2	22.571	350	187	9	48	316	53	399	3.95e-12	67.4
MsaG007769	AT1G61970.7	22.571	350	187	9	48	316	53	399	3.95e-12	67.4
MsaG007769	AT1G61970.6	22.571	350	187	9	48	316	53	399	3.95e-12	67.4
MsaG007769	AT1G61970.3	22.571	350	187	9	48	316	53	399	3.95e-12	67.4
MsaG007769	AT1G61970.5	22.571	350	187	9	48	316	53	399	3.95e-12	67.4
MsaG007769	AT1G61970.4	22.571	350	187	9	48	316	53	399	3.95e-12	67.4
MsaG007769	AT1G61970.1	22.571	350	187	9	48	316	53	399	3.95e-12	67.4

Find 60 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGAACCATTTCGTTATTC+TGG	0.141446	2:+22694399	MsaT007769.1:CDS
ATCAATTTAATGTCTGATTT+TGG	0.143567	2:+22693937	MsaT007769.1:CDS
TCATACCTCATCAACAATTT+TGG	0.165317	2:+22693511	MsaT007769.1:CDS
TTCAATTGATAAGATTAATT+TGG	0.211307	2:+22694218	MsaT007769.1:CDS
TGGAGGAAGTTAAGAGTTTA+GGG	0.232380	2:+22694037	MsaT007769.1:CDS
TTGGAGGAAGTTAAGAGTTT+AGG	0.257551	2:+22694036	MsaT007769.1:CDS
ATTCCTTGGCACTTGCTAAA+AGG	0.292312	2:+22694274	MsaT007769.1:CDS
AAGAAGGTGAGAACTGAATT+AGG	0.306086	2:-22693601	None:intergenic
AAAGGATTTGATTAAGTCAT+TGG	0.323129	2:+22694017	MsaT007769.1:CDS
AATTGATATTAGACACAATA+AGG	0.329025	2:-22693921	None:intergenic
GATCCTTGGACGAATACTTT+TGG	0.330015	2:+22694063	MsaT007769.1:CDS
TAGACCAACCCCATTTCTTA+AGG	0.338165	2:-22694146	None:intergenic
CAGTCAAGGCCATCTACATT+TGG	0.353370	2:+22693991	MsaT007769.1:CDS
ATTAGACACAATAAGGTGAT+AGG	0.358420	2:-22693914	None:intergenic
CAAGTTACACATTCGGTAGA+TGG	0.370763	2:-22694540	None:intergenic
AAGTTGATGCCCTTAAGAAA+TGG	0.388575	2:+22694136	MsaT007769.1:CDS
TAGTATCTGATGAGTGAAAT+CGG	0.388813	2:-22693436	None:intergenic
AGAGTTTAGGGTTTGATCCT+TGG	0.397034	2:+22694049	MsaT007769.1:CDS
AGTTGATGCCCTTAAGAAAT+GGG	0.397811	2:+22694137	MsaT007769.1:CDS
TTGAACCATTTCGTTATTCT+GGG	0.398296	2:+22694400	MsaT007769.1:CDS
TCCTTTGAACCAAATGTAGA+TGG	0.407709	2:-22694000	None:intergenic
GCAAACAATGGTATCCTTGT+CGG	0.427133	2:-22693857	None:intergenic
GCCATCTACATTTGGTTCAA+AGG	0.443477	2:+22693999	MsaT007769.1:CDS
AGACCAACCCCATTTCTTAA+GGG	0.447273	2:-22694145	None:intergenic
AACAATTGTTCCAAGGGCAT+TGG	0.458416	2:+22694326	MsaT007769.1:CDS
TCAATTGGGTGGGAATTCCT+TGG	0.472262	2:+22694260	MsaT007769.1:CDS
TTTCATCACAACCCCACAAA+AGG	0.473339	2:+22693686	MsaT007769.1:CDS
CTGCAATATCTTGTGATGAA+AGG	0.485086	2:+22694351	MsaT007769.1:CDS
ATCTTGTGATGAAAGGTTTG+AGG	0.509536	2:+22694358	MsaT007769.1:CDS
TGCAGAAAACAATTGTTCCA+AGG	0.522766	2:+22694319	MsaT007769.1:CDS
TGTTTAACAAGTTACACATT+CGG	0.526635	2:-22694547	None:intergenic
CAAGCATTTAGGATGCATCC+TGG	0.527087	2:+22694183	MsaT007769.1:CDS
ACACATTCGGTAGATGGCAT+TGG	0.528449	2:-22694534	None:intergenic
TTGTTTGCTTAATTCGATGT+TGG	0.530080	2:+22693872	MsaT007769.1:CDS
GCAGAAAACAATTGTTCCAA+GGG	0.535861	2:+22694320	MsaT007769.1:CDS
CAAAGCTTTCAACAACAAGC+AGG	0.537532	2:+22693555	MsaT007769.1:CDS
AGTATCTGATGAGTGAAATC+GGG	0.544517	2:-22693435	None:intergenic
GAGACCTAAAACTACATTTG+TGG	0.545867	2:-22693384	None:intergenic
AAAGCTTTGAGAGCAGATTG+AGG	0.549590	2:-22693541	None:intergenic
TATTGCAGAACCAATGCCCT+TGG	0.552410	2:-22694336	None:intergenic
TTTGGGTTAATCAATTGGGT+GGG	0.559000	2:+22694250	MsaT007769.1:CDS
TCGGGAACAACAAGTGTTGA+GGG	0.560234	2:-22693417	None:intergenic
AGAAACCAAAATTGTTGATG+AGG	0.561539	2:-22693516	None:intergenic
ATCGGGAACAACAAGTGTTG+AGG	0.562201	2:-22693418	None:intergenic
TTAGACACAATAAGGTGATA+GGG	0.570881	2:-22693913	None:intergenic
GGATTTGATTAAGTCATTGG+AGG	0.571043	2:+22694020	MsaT007769.1:CDS
CTCACCTTCTTAGAAAACCA+TGG	0.576168	2:+22693613	MsaT007769.1:CDS
TTGCTAGCTCGCTTCAGTCA+AGG	0.593225	2:+22693977	MsaT007769.1:CDS
ATTCCAAAAGTATTCGTCCA+AGG	0.607263	2:-22694066	None:intergenic
ATGAGGTATGATACAGCGAA+TGG	0.607723	2:-22693499	None:intergenic
GAGAAACAATTTCTTCAACA+TGG	0.614078	2:-22693359	None:intergenic
ATGCCCTTAAGAAATGGGGT+TGG	0.630132	2:+22694142	MsaT007769.1:CDS
ACCTAAAACTACATTTGTGG+CGG	0.636797	2:-22693381	None:intergenic
GTTGATGCCCTTAAGAAATG+GGG	0.641680	2:+22694138	MsaT007769.1:CDS
ATTGAAGTCAACATGAAACC+AGG	0.642061	2:-22694201	None:intergenic
ACATCGAATTAAGCAAACAA+TGG	0.642451	2:-22693869	None:intergenic
TGAGGTATGATACAGCGAAT+GGG	0.657928	2:-22693498	None:intergenic
TAGGTTCTTTAAAACCGACA+AGG	0.672846	2:+22693843	MsaT007769.1:intron
GCATACACAAAAGAGAACAA+TGG	0.682398	2:+22694507	MsaT007769.1:CDS
GGTTGTGATGAAAGTAACCA+AGG	0.698597	2:-22693676	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	22693361	22694566	22693361	ID=MsaG007769
Chr2	mRNA	22693361	22694566	22693361	ID=MsaT007769.1;Parent=MsaG007769
Chr2	exon	22693361	22693707	22693361	ID=MsaT007769.1.exon1;Parent=MsaT007769.1
Chr2	CDS	22693361	22693707	22693361	ID=cds.MsaT007769.1;Parent=MsaT007769.1
Chr2	exon	22693846	22694566	22693846	ID=MsaT007769.1.exon2;Parent=MsaT007769.1
Chr2	CDS	22693846	22694566	22693846	ID=cds.MsaT007769.1;Parent=MsaT007769.1

Gene Sequence

>MsaT007769.1

ATGTTGAAGAAATTGTTTCTCCGCCACAAATGTAGTTTTAGGTCTCATCACAACAACCCTCAACACTTGTTGTTCCCGATTTCACTCATCAGATACTACAACACTACAATAACAACAACATCTGAATCAAACACACACCCATTCGCTGTATCATACCTCATCAACAATTTTGGTTTCTCACCTCAATCTGCTCTCAAAGCTTTCAACAACAAGCAGGTTCGTTTCAATACCCTTGAAAAGCCTAATTCAGTTCTCACCTTCTTAGAAAACCATGGTTTTTCACACGACAACATACGCGTCATCATCAAAAAAGAACCTTGGTTACTTTCATCACAACCCCACAAAAGGTTCTTTAAAACCGACAAGGATACCATTGTTTGCTTAATTCGATGTTGGACTGCACTTGCTATTAATCCCTATCACCTTATTGTGTCTAATATCAATTTAATGTCTGATTTTGGAGTTTCTGATTCTGTCATTGCTAGCTCGCTTCAGTCAAGGCCATCTACATTTGGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGAGTTTAGGGTTTGATCCTTGGACGAATACTTTTGGAATTGCTTTGGCAGCTAAGAAAAGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATGCCCTTAAGAAATGGGGTTGGTCTAATGAAGCTGTTTTTCAAGCATTTAGGATGCATCCTGGTTTCATGTTGACTTCAATTGATAAGATTAATTTGGTGATGAGTTTTTGGGTTAATCAATTGGGTGGGAATTCCTTGGCACTTGCTAAAAGGCCACATATTTTTGATTCAAGTTTGCAGAAAACAATTGTTCCAAGGGCATTGGTTCTGCAATATCTTGTGATGAAAGGTTTGAGGAAAAAGAATGCAAGCTTAGTTGAACCATTTCGTTATTCTGGGAAAATGTTCTTGAGCAAGTTTGTTTTCAGCTTTAAGGAGGAGTCTGATTATCTATTAAAGTTATATGACGAAAAAATGAAACTTGCATACACAAAAGAGAACAATGGCATGCCAATGCCATCTACCGAATGTGTAACTTGTTAA

Protein sequence

>MsaT007769.1

MLKKLFLRHKCSFRSHHNNPQHLLFPISLIRYYNTTITTTSESNTHPFAVSYLINNFGFSPQSALKAFNNKQVRFNTLEKPNSVLTFLENHGFSHDNIRVIIKKEPWLLSSQPHKRFFKTDKDTIVCLIRCWTALAINPYHLIVSNINLMSDFGVSDSVIASSLQSRPSTFGSKDLIKSLEEVKSLGFDPWTNTFGIALAAKKSLSKKLWDEKVDALKKWGWSNEAVFQAFRMHPGFMLTSIDKINLVMSFWVNQLGGNSLALAKRPHIFDSSLQKTIVPRALVLQYLVMKGLRKKNASLVEPFRYSGKMFLSKFVFSFKEESDYLLKLYDEKMKLAYTKENNGMPMPSTECVTC*