AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007770 MtrunA17_Chr2g0296231 90.796 402 31 1 1 402 1 396 0.0 729
MsaG007770 MtrunA17_Chr2g0296221 72.208 403 108 2 1 399 25 427 0.0 573
MsaG007770 MtrunA17_Chr2g0296191 71.576 387 106 2 1 383 608 994 0.0 551
MsaG007770 MtrunA17_Chr2g0296191 58.774 359 135 5 31 385 160 509 1.23e-129 398
MsaG007770 MtrunA17_Chr2g0296711 72.818 401 105 2 1 399 1 399 0.0 544
MsaG007770 MtrunA17_Chr2g0296721 68.812 404 116 4 1 401 1 397 0.0 533
MsaG007770 MtrunA17_Chr2g0315731 66.501 403 128 2 1 401 21 418 0.0 517
MsaG007770 MtrunA17_Chr2g0296741 64.268 403 137 2 1 401 21 418 2.92e-179 505
MsaG007770 MtrunA17_Chr2g0296171 69.036 394 109 4 1 386 7 395 6.72e-178 501
MsaG007770 MtrunA17_Chr2g0296181 65.174 402 107 4 1 400 1 371 6.28e-172 486
MsaG007770 MtrunA17_Chr2g0296291 60.250 400 114 4 36 398 19 410 2.42e-156 447
MsaG007770 MtrunA17_Chr2g0296251 59.424 382 114 4 42 386 21 398 7.04e-147 422
MsaG007770 MtrunA17_Chr2g0296151 52.162 370 167 5 31 398 21 382 1.07e-123 363
MsaG007770 MtrunA17_Chr2g0296111 53.209 374 159 7 29 394 13 378 5.27e-121 356
MsaG007770 MtrunA17_Chr2g0295741 49.211 380 183 4 21 398 17 388 3.16e-118 349
MsaG007770 MtrunA17_Chr2g0296301 48.596 356 172 6 42 393 25 373 9.60e-112 332
MsaG007770 MtrunA17_Chr2g0296121 46.787 389 192 5 12 398 93 468 2.40e-109 329
MsaG007770 MtrunA17_Chr2g0295731 48.641 368 172 6 25 386 21 377 4.45e-109 325
MsaG007770 MtrunA17_Chr2g0295761 50.432 347 160 6 44 387 53 390 9.93e-104 312
MsaG007770 MtrunA17_Chr4g0067121 46.610 354 175 5 44 394 46 388 4.70e-102 308
MsaG007770 MtrunA17_Chr2g0296551 72.362 199 53 1 205 401 1 199 2.54e-97 288
MsaG007770 MtrunA17_Chr4g0067671 44.479 326 174 3 46 371 37 355 4.56e-87 268
MsaG007770 MtrunA17_Chr2g0296211 68.617 188 57 1 217 402 187 374 8.87e-85 263
MsaG007770 MtrunA17_Chr2g0296211 61.481 135 49 1 1 135 66 197 1.03e-43 156
MsaG007770 MtrunA17_Chr4g0067141 42.857 343 187 4 43 383 34 369 1.45e-84 262
MsaG007770 MtrunA17_Chr2g0296701 74.118 170 42 1 234 401 2 171 2.00e-84 255
MsaG007770 MtrunA17_Chr2g0286191 42.183 339 175 8 57 386 33 359 9.04e-72 229
MsaG007770 MtrunA17_Chr2g0296751 72.222 144 37 2 260 401 1 143 3.69e-62 197
MsaG007770 MtrunA17_Chr2g0296131 55.422 166 72 1 235 398 9 174 2.46e-58 188
MsaG007770 MtrunA17_Chr2g0296161 52.047 171 80 1 231 399 1 171 1.37e-56 183
MsaG007770 MtrunA17_Chr4g0001831 28.775 351 240 6 38 383 22 367 9.73e-40 145
MsaG007770 MtrunA17_Chr2g0295721 56.637 113 49 0 231 343 1 113 5.13e-39 135
MsaG007770 MtrunA17_Chr2g0295711 42.771 166 88 3 42 207 37 195 7.52e-34 125
MsaG007770 MtrunA17_Chr2g0286221 46.795 156 79 3 233 386 16 169 1.24e-33 124
MsaG007770 MtrunA17_Chr2g0295751 47.170 159 77 2 28 183 21 175 1.18e-32 128
MsaG007770 MtrunA17_Chr2g0296561 46.296 162 67 6 2 163 1 142 2.13e-30 114
MsaG007770 MtrunA17_Chr8g0377781 31.773 299 194 5 88 380 74 368 2.70e-30 120
MsaG007770 MtrunA17_Chr8g0377801 28.146 302 211 3 86 383 85 384 8.95e-29 116
MsaG007770 MtrunA17_Chr4g0067131 51.852 81 37 1 304 382 1 81 1.48e-20 85.5
MsaG007770 MtrunA17_Chr4g0067681 53.333 60 28 0 293 352 1 60 2.51e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007770 AT5G07900.1 31.025 361 240 7 30 384 43 400 1.17e-52 181
MsaG007770 AT1G21150.1 31.595 326 214 4 57 377 57 378 3.90e-46 163
MsaG007770 AT1G21150.3 31.138 334 221 4 49 377 13 342 1.01e-45 161
MsaG007770 AT1G21150.2 31.595 326 214 4 57 377 121 442 2.53e-45 162
MsaG007770 AT1G61970.2 27.119 354 212 9 57 371 53 399 6.04e-30 120
MsaG007770 AT1G61970.7 27.119 354 212 9 57 371 53 399 6.04e-30 120
MsaG007770 AT1G61970.6 27.119 354 212 9 57 371 53 399 6.04e-30 120
MsaG007770 AT1G61970.3 27.119 354 212 9 57 371 53 399 6.04e-30 120
MsaG007770 AT1G61970.5 27.119 354 212 9 57 371 53 399 6.04e-30 120
MsaG007770 AT1G61970.4 27.119 354 212 9 57 371 53 399 6.04e-30 120
MsaG007770 AT1G61970.1 27.119 354 212 9 57 371 53 399 6.04e-30 120
MsaG007770 AT1G61990.2 28.056 360 200 8 57 373 53 396 7.48e-30 119
MsaG007770 AT1G61990.1 28.056 360 200 8 57 373 53 396 7.48e-30 119
MsaG007770 AT1G61980.3 25.549 364 221 8 57 379 53 407 4.75e-28 114
MsaG007770 AT1G61980.2 25.549 364 221 8 57 379 53 407 4.75e-28 114
MsaG007770 AT1G61980.1 25.549 364 221 8 57 379 53 407 4.75e-28 114
MsaG007770 AT5G64950.1 26.891 357 247 7 38 383 22 375 3.50e-26 109
MsaG007770 AT3G46950.1 28.358 268 181 5 35 300 30 288 2.34e-25 107
MsaG007770 AT1G62120.1 25.956 366 220 8 57 386 57 407 7.22e-25 105
MsaG007770 AT1G62150.1 23.058 399 230 10 57 382 59 453 9.48e-22 97.1
MsaG007770 AT1G62010.1 25.150 334 197 6 89 386 69 385 8.21e-21 94.0
MsaG007770 AT1G61960.1 28.862 246 170 4 57 301 53 294 1.06e-20 94.0
MsaG007770 AT1G62110.1 24.444 360 244 9 34 381 30 373 4.97e-18 86.3
MsaG007770 AT5G23930.1 22.642 424 243 8 27 375 22 435 2.00e-16 81.3
MsaG007770 AT1G62085.1 25.301 332 230 9 57 381 56 376 3.74e-16 80.5
MsaG007770 AT1G62085.2 25.301 332 230 9 57 381 56 376 3.74e-16 80.5
MsaG007770 AT1G56380.1 24.121 398 221 15 55 395 15 388 3.77e-14 73.9
MsaG007770 AT1G56380.2 24.121 398 221 15 55 395 26 399 3.98e-14 73.9

Find 70 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAGCAAGTTTGTCTTTA+AGG 0.043675 2:+22702846 MsaT007770.1:CDS
ATCAATTTGATGACTGATTT+TGG 0.089088 2:+22702352 MsaT007770.1:CDS
AAAAGAGGGTTGTTGAATTT+TGG 0.120653 2:-22701815 None:intergenic
CTGAAAAGGCCATCTATATT+TGG 0.156515 2:+22702406 MsaT007770.1:CDS
AAGAAATTGATAACTGAATT+AGG 0.176367 2:-22702016 None:intergenic
GAGAATCTTGTGAGTGAAAA+TGG 0.247387 2:-22701857 None:intergenic
GATCCTTCAATGGCTACTTT+TGG 0.277843 2:+22702478 MsaT007770.1:CDS
TTGGAAGAAGTTCATGGTTT+AGG 0.289669 2:+22702451 MsaT007770.1:CDS
TGGCATGCCATTCACTAATT+AGG 0.298571 2:+22702936 MsaT007770.1:CDS
GAAAATGGGAATGAGAAGTT+TGG 0.301232 2:-22701842 None:intergenic
CTTTCCCAGAATGTTGCTTT+AGG 0.307195 2:+22701767 MsaT007770.1:CDS
TGATGCCTAAAGCAACATTC+TGG 0.327907 2:-22701772 None:intergenic
CCAATTGGGTTGGGATTCCT+TGG 0.333577 2:+22702675 MsaT007770.1:CDS
TCCAAACCATGTTGAAGAAA+TGG 0.334748 2:+22701741 None:intergenic
AACTGATTTGATTAAGTCAT+TGG 0.339262 2:+22702432 MsaT007770.1:CDS
GCAAAGAATGATATCCTTGT+TGG 0.359776 2:-22702272 None:intergenic
TTGTTCTCCTTTGTGTATGC+AGG 0.375653 2:-22702913 None:intergenic
ACTAAATGTCCACATATGTT+TGG 0.382004 2:+22702703 MsaT007770.1:CDS
CAAAGCTTTCAACAACAATC+AGG 0.385654 2:+22701970 MsaT007770.1:CDS
ATCAGATGAAGTAGCACCTT+TGG 0.385922 2:-22702152 None:intergenic
GAGAAGTTTGGGTTCAAAAG+AGG 0.413500 2:-22701830 None:intergenic
TTGGGTCAACCAATTGGGTT+GGG 0.418889 2:+22702666 MsaT007770.1:CDS
CTTGGGCAAAATTATATTGT+GGG 0.422253 2:-22702113 None:intergenic
AAAATGGGAATGAGAAGTTT+GGG 0.423820 2:-22701841 None:intergenic
ATTAGCCACAACAAGGTGAT+AGG 0.433161 2:-22702329 None:intergenic
AAGTCATTGGAAGAAGTTCA+TGG 0.433750 2:+22702445 MsaT007770.1:CDS
TTTCTAGATTGCTTCTGAAA+AGG 0.437099 2:+22702392 MsaT007770.1:CDS
TCAGTTGAACCAAATATAGA+TGG 0.441181 2:-22702415 None:intergenic
CCAAGGAATCCCAACCCAAT+TGG 0.447231 2:-22702675 None:intergenic
TTTAGGATTTGATCCTTCAA+TGG 0.450730 2:+22702468 MsaT007770.1:CDS
TCTAATGATGATTCGTATGT+CGG 0.457132 2:-22702062 None:intergenic
TCGCAAAATTCGAGGGTTTG+CGG 0.479537 2:-22702191 None:intergenic
AGAAGTTTGGGTTCAAAAGA+GGG 0.481605 2:-22701829 None:intergenic
GTTGCATCCTAGTTTGATGT+TGG 0.488218 2:+22702609 MsaT007770.1:CDS
GATGCCTAAAGCAACATTCT+GGG 0.494312 2:-22701771 None:intergenic
TTTGGGTCAACCAATTGGGT+TGG 0.496217 2:+22702665 MsaT007770.1:CDS
AAGCTACTTCGCAAAATTCG+AGG 0.497556 2:-22702199 None:intergenic
TCCCCAAAAGTAGCCATTGA+AGG 0.500530 2:-22702481 None:intergenic
ACTTGGGCAAAATTATATTG+TGG 0.500709 2:-22702114 None:intergenic
TTGGTTATAGTTTACATCAA+AGG 0.507419 2:+22702722 MsaT007770.1:CDS
TAATTCCTATCACCTTGTTG+TGG 0.508203 2:+22702324 MsaT007770.1:CDS
AAAGCTTTGAGAGCAGATTG+AGG 0.511231 2:-22701956 None:intergenic
GAAATCACCTAATTAGTGAA+TGG 0.511658 2:-22702943 None:intergenic
AATTTCTCAGAATGAGCAAA+TGG 0.512809 2:-22702820 None:intergenic
AATTGCAAAACTGAGGCCCT+TGG 0.520839 2:-22702751 None:intergenic
AGAATCTTGTGAGTGAAAAT+GGG 0.534017 2:-22701856 None:intergenic
AAACTATAACCAAACATATG+TGG 0.543018 2:-22702712 None:intergenic
TTCTGACTCTGATGTTGTGG+TGG 0.553341 2:-22701879 None:intergenic
ATTGAAGCCAACATCAAACT+AGG 0.554840 2:-22702616 None:intergenic
TGATTCTGACTCTGATGTTG+TGG 0.556453 2:-22701882 None:intergenic
TTATCTATTAAAGCTATATG+TGG 0.557272 2:+22702879 MsaT007770.1:CDS
TACATCAAAGGATCATTCCA+AGG 0.557901 2:+22702734 MsaT007770.1:CDS
TGAAATCAAAATTGTTGATG+AGG 0.559601 2:-22701931 None:intergenic
AGCTACTTCGCAAAATTCGA+GGG 0.561434 2:-22702198 None:intergenic
ATGAGGTACGATACAGCAAA+TGG 0.565479 2:-22701914 None:intergenic
TTGGGCAAAATTATATTGTG+GGG 0.586920 2:-22702112 None:intergenic
AAGAAACCATTTCTTCAACA+TGG 0.592220 2:-22701747 None:intergenic
TGGACATTTAGTAAGTTCCA+AGG 0.592713 2:-22702692 None:intergenic
GAGCAAGTTTGTCTTTAAGG+AGG 0.592819 2:+22702849 MsaT007770.1:CDS
ATGTATGAGTAAAACACGGT+GGG 0.613074 2:+22702522 MsaT007770.1:CDS
ACATCAAAGGATCATTCCAA+GGG 0.634178 2:+22702735 MsaT007770.1:CDS
CAAAAGAAATTGCAAAACTG+AGG 0.646915 2:-22702758 None:intergenic
AATTGATATTAGCCACAACA+AGG 0.659212 2:-22702336 None:intergenic
AATGAAACCTGCATACACAA+AGG 0.664808 2:+22702906 MsaT007770.1:CDS
GGTTGTAATGAAAGTAACCA+TGG 0.672923 2:-22702091 None:intergenic
TCATCATTAGAAAAGCGCCA+TGG 0.688476 2:+22702074 MsaT007770.1:CDS
AGGTACGATACAGCAAATGG+TGG 0.691130 2:-22701911 None:intergenic
GCATACACAAAGGAGAACAA+TGG 0.692068 2:+22702916 MsaT007770.1:CDS
AATGTATGAGTAAAACACGG+TGG 0.729131 2:+22702521 MsaT007770.1:CDS
AGAAATGTATGAGTAAAACA+CGG 0.759128 2:+22702518 MsaT007770.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22701749 22702957 22701749 ID=MsaG007770
Chr2 mRNA 22701749 22702957 22701749 ID=MsaT007770.1;Parent=MsaG007770
Chr2 exon 22701749 22702957 22701749 ID=MsaT007770.1.exon1;Parent=MsaT007770.1
Chr2 CDS 22701749 22702957 22701749 ID=cds.MsaT007770.1;Parent=MsaT007770.1
Gene Sequence

>MsaT007770.1

ATGTTGAAGAAATGGTTTCTTTCCCAGAATGTTGCTTTAGGCATCATCACCACAAAATCAACAACCCCAAAATTCAACAACCCTCTTTTGAACCCAAACTTCTCATTCCCATTTTCACTCACAAGATTCTCCACCACAACATCAGAGTCAGAATCAGTCACACCACCATTTGCTGTATCGTACCTCATCAACAATTTTGATTTCACTCCTCAATCTGCTCTCAAAGCTTTCAACAACAATCAGGTTCTTTTCAAATCTGCAGAAAATCCTAATTCAGTTATCAATTTCTTCAAAAACCGTGATTTTTCACACTCCGACATACGAATCATCATTAGAAAAGCGCCATGGTTACTTTCATTACAACCCCACAATATAATTTTGCCCAAGTTTGAATTTTTTCTATCCAAAGGTGCTACTTCATCTGATATTGTTTCATTCATAACCGCAAACCCTCGAATTTTGCGAAGTAGCTTGGAGAAACGAATAATCCCTCTTTTTCAATTGTTCAGTAGGTTTTTGAAAACCAACAAGGATATCATTCTTTGCTTACTTCGACATTCGACTTATGTTACTTTTAATTCCTATCACCTTGTTGTGGCTAATATCAATTTGATGACTGATTTTGGAGTTTCTGATTCTGTCATTTCTAGATTGCTTCTGAAAAGGCCATCTATATTTGGTTCAACTGATTTGATTAAGTCATTGGAAGAAGTTCATGGTTTAGGATTTGATCCTTCAATGGCTACTTTTGGGGATGCTTTGTTTGCCAAGAAATGTATGAGTAAAACACGGTGGGATGAGAAAGTTGATGTTTTTAAGAAATGGGGTTGGTTTGATGAAGCTTTTTTTCAAGCATTTAGGTTGCATCCTAGTTTGATGTTGGCTTCAATTGAAAAGATTAATTTTGTGATGCATTTTTGGGTCAACCAATTGGGTTGGGATTCCTTGGAACTTACTAAATGTCCACATATGTTTGGTTATAGTTTACATCAAAGGATCATTCCAAGGGCCTCAGTTTTGCAATTTCTTTTGATGAAAGGTTTGCGAGATAAGAATGAAAGCTTAGTTACACCATTTGCTCATTCTGAGAAATTTTTCTTGAGCAAGTTTGTCTTTAAGGAGGAGTCTGATTATCTATTAAAGCTATATGTGGAAAAAATGAAACCTGCATACACAAAGGAGAACAATGGCATGCCATTCACTAATTAG
Protein sequence

>MsaT007770.1

MLKKWFLSQNVALGIITTKSTTPKFNNPLLNPNFSFPFSLTRFSTTTSESESVTPPFAVSYLINNFDFTPQSALKAFNNNQVLFKSAENPNSVINFFKNRDFSHSDIRIIIRKAPWLLSLQPHNIILPKFEFFLSKGATSSDIVSFITANPRILRSSLEKRIIPLFQLFSRFLKTNKDIILCLLRHSTYVTFNSYHLVVANINLMTDFGVSDSVISRLLLKRPSIFGSTDLIKSLEEVHGLGFDPSMATFGDALFAKKCMSKTRWDEKVDVFKKWGWFDEAFFQAFRLHPSLMLASIEKINFVMHFWVNQLGWDSLELTKCPHMFGYSLHQRIIPRASVLQFLLMKGLRDKNESLVTPFAHSEKFFLSKFVFKEESDYLLKLYVEKMKPAYTKENNGMPFTN*