AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007771

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007771 MtrunA17_Chr2g0296291 78.870 407 80 2 236 642 10 410 0.0 641
MsaG007771 MtrunA17_Chr2g0296291 81.308 107 20 0 1 107 215 321 2.55e-51 183
MsaG007771 MtrunA17_Chr2g0296251 73.671 395 98 2 236 630 10 398 0.0 583
MsaG007771 MtrunA17_Chr2g0296251 72.897 107 29 0 1 107 215 321 8.91e-45 164
MsaG007771 MtrunA17_Chr2g0296221 65.239 397 99 3 251 643 66 427 2.39e-176 508
MsaG007771 MtrunA17_Chr2g0296221 76.636 107 25 0 1 107 231 337 1.47e-48 176
MsaG007771 MtrunA17_Chr2g0296171 65.979 388 89 4 251 630 43 395 8.92e-166 480
MsaG007771 MtrunA17_Chr2g0296171 74.766 107 27 0 1 107 212 318 1.95e-45 166
MsaG007771 MtrunA17_Chr2g0296711 63.351 382 103 2 264 643 53 399 2.40e-163 474
MsaG007771 MtrunA17_Chr2g0296711 72.897 107 29 0 1 107 203 309 7.13e-45 165
MsaG007771 MtrunA17_Chr2g0296741 59.569 418 123 3 239 645 36 418 1.97e-161 469
MsaG007771 MtrunA17_Chr2g0296741 57.812 128 54 0 1 128 220 347 2.37e-41 155
MsaG007771 MtrunA17_Chr2g0296231 61.558 398 108 4 251 646 42 396 4.66e-161 468
MsaG007771 MtrunA17_Chr2g0296231 72.897 107 29 0 1 107 199 305 1.53e-45 166
MsaG007771 MtrunA17_Chr2g0315731 59.905 419 122 3 238 645 35 418 1.01e-158 462
MsaG007771 MtrunA17_Chr2g0315731 59.504 121 49 0 1 121 220 340 4.24e-39 149
MsaG007771 MtrunA17_Chr2g0296721 59.189 419 124 4 238 645 15 397 9.06e-158 459
MsaG007771 MtrunA17_Chr2g0296721 57.812 128 54 0 1 128 199 326 1.01e-40 153
MsaG007771 MtrunA17_Chr2g0296191 64.042 381 98 3 251 627 649 994 5.74e-156 476
MsaG007771 MtrunA17_Chr2g0296191 56.065 371 125 2 259 629 177 509 8.84e-130 407
MsaG007771 MtrunA17_Chr2g0296191 76.636 107 25 0 1 107 814 920 2.60e-46 176
MsaG007771 MtrunA17_Chr2g0296191 62.857 105 39 0 3 107 329 433 3.76e-36 145
MsaG007771 MtrunA17_Chr2g0296181 58.629 394 99 4 253 644 40 371 3.83e-145 427
MsaG007771 MtrunA17_Chr2g0296181 65.657 99 33 1 1 99 201 298 1.70e-33 132
MsaG007771 MtrunA17_Chr2g0296301 52.099 405 152 5 232 634 6 370 2.32e-136 404
MsaG007771 MtrunA17_Chr2g0296301 43.119 109 60 1 1 107 181 289 1.64e-23 103
MsaG007771 MtrunA17_Chr2g0296151 49.875 399 156 4 244 640 24 380 1.34e-126 379
MsaG007771 MtrunA17_Chr2g0296151 57.282 103 44 0 5 107 191 293 3.69e-33 131
MsaG007771 MtrunA17_Chr2g0295741 49.626 401 158 4 244 642 30 388 6.34e-126 377
MsaG007771 MtrunA17_Chr2g0295741 54.369 103 47 0 5 107 197 299 6.09e-28 116
MsaG007771 MtrunA17_Chr2g0296111 50.882 397 148 6 246 638 25 378 2.62e-122 368
MsaG007771 MtrunA17_Chr2g0296111 59.048 105 41 1 5 107 190 294 6.27e-31 125
MsaG007771 MtrunA17_Chr4g0067121 47.244 381 162 3 259 638 46 388 1.38e-113 346
MsaG007771 MtrunA17_Chr4g0067121 50.476 105 52 0 3 107 199 303 3.17e-28 117
MsaG007771 MtrunA17_Chr2g0296121 47.103 397 166 4 248 642 114 468 4.32e-112 345
MsaG007771 MtrunA17_Chr2g0296121 59.048 105 43 0 3 107 275 379 3.31e-32 130
MsaG007771 MtrunA17_Chr2g0295731 46.809 376 160 4 261 634 44 381 2.36e-106 327
MsaG007771 MtrunA17_Chr2g0295731 58.252 103 43 0 5 107 198 300 1.30e-32 129
MsaG007771 MtrunA17_Chr2g0296551 77.387 199 45 0 447 645 1 199 4.42e-105 317
MsaG007771 MtrunA17_Chr2g0296551 78.505 107 23 0 1 107 1 107 3.44e-51 176
MsaG007771 MtrunA17_Chr2g0295761 45.600 375 165 5 258 631 54 390 2.34e-96 301
MsaG007771 MtrunA17_Chr2g0295761 47.170 106 55 1 3 107 207 312 2.02e-22 100
MsaG007771 MtrunA17_Chr2g0296211 73.936 188 49 0 459 646 187 374 1.58e-92 291
MsaG007771 MtrunA17_Chr2g0296211 67.021 94 31 0 14 107 188 281 2.97e-35 137
MsaG007771 MtrunA17_Chr2g0296211 32.683 205 86 3 251 406 104 305 2.67e-22 99.4
MsaG007771 MtrunA17_Chr4g0067141 42.971 377 176 3 252 628 33 370 5.44e-92 289
MsaG007771 MtrunA17_Chr4g0067141 48.571 105 54 0 3 107 191 295 2.17e-25 108
MsaG007771 MtrunA17_Chr4g0067671 43.662 355 162 2 259 613 39 355 7.20e-89 281
MsaG007771 MtrunA17_Chr4g0067671 49.580 119 60 0 3 121 191 309 5.40e-29 119
MsaG007771 MtrunA17_Chr2g0296701 75.882 170 41 0 476 645 2 171 6.90e-83 258
MsaG007771 MtrunA17_Chr2g0296701 73.077 78 21 0 30 107 2 79 1.81e-32 123
MsaG007771 MtrunA17_Chr2g0286191 42.021 376 166 7 262 630 29 359 8.97e-78 252
MsaG007771 MtrunA17_Chr2g0286191 50.000 94 41 2 14 107 196 283 3.02e-16 80.9
MsaG007771 MtrunA17_Chr2g0296751 77.778 144 31 1 502 645 1 143 3.00e-70 225
MsaG007771 MtrunA17_Chr2g0296751 78.846 52 11 0 56 107 1 52 7.00e-22 93.2
MsaG007771 MtrunA17_Chr2g0296131 57.895 171 72 0 472 642 4 174 6.55e-64 208
MsaG007771 MtrunA17_Chr2g0296131 59.756 82 33 0 26 107 4 85 1.58e-25 103
MsaG007771 MtrunA17_Chr2g0296161 55.556 171 76 0 473 643 1 171 1.20e-62 205
MsaG007771 MtrunA17_Chr2g0296161 56.790 81 35 0 27 107 1 81 1.09e-26 107
MsaG007771 MtrunA17_Chr2g0296561 69.799 149 35 2 236 380 10 152 8.83e-61 199
MsaG007771 MtrunA17_Chr4g0001831 29.076 368 222 4 264 628 37 368 4.84e-44 162
MsaG007771 MtrunA17_Chr2g0286221 48.466 163 82 2 475 637 16 176 7.75e-42 149
MsaG007771 MtrunA17_Chr2g0286221 42.424 99 56 1 20 118 7 104 5.68e-16 76.6
MsaG007771 MtrunA17_Chr2g0295721 58.407 113 47 0 473 585 1 113 6.55e-40 142
MsaG007771 MtrunA17_Chr2g0295721 62.963 81 30 0 27 107 1 81 2.34e-27 107
MsaG007771 MtrunA17_Chr2g0295751 41.758 182 65 3 246 425 33 175 2.01e-31 128
MsaG007771 MtrunA17_Chr2g0295711 36.788 193 84 3 235 425 18 174 1.36e-30 119
MsaG007771 MtrunA17_Chr8g0377781 28.012 332 200 4 295 624 74 368 7.15e-29 119
MsaG007771 MtrunA17_Chr8g0377801 25.148 338 214 3 293 628 85 385 6.83e-28 116
MsaG007771 MtrunA17_Chr6g0480701 41.935 124 71 1 108 230 608 731 3.42e-22 101
MsaG007771 MtrunA17_Chr4g0067131 51.852 81 39 0 546 626 1 81 1.25e-21 89.7
MsaG007771 MtrunA17_Chr2g0289581 37.168 113 70 1 122 234 2 113 4.16e-19 84.0
MsaG007771 MtrunA17_Chr3g0134051 39.091 110 66 1 122 230 2 111 9.93e-19 84.0
MsaG007771 MtrunA17_Chr4g0067681 54.098 61 28 0 535 595 1 61 1.16e-15 72.0
MsaG007771 MtrunA17_Chr4g0011231 38.542 96 57 2 109 202 103 198 1.89e-14 73.2
MsaG007771 MtrunA17_Chr7g0256881 37.190 121 65 2 115 234 1306 1416 8.81e-14 75.1
MsaG007771 MtrunA17_Chr2g0281001 32.787 122 78 3 93 211 2 122 1.64e-13 70.1
MsaG007771 MtrunA17_Chr6g0456371 31.176 170 113 2 112 281 21 186 1.72e-12 67.4
MsaG007771 MtrunA17_Chr8g0339041 20.065 309 238 5 344 644 63 370 1.78e-12 69.3
MsaG007771 MtrunA17_Chr3g0132221 27.273 121 88 0 114 234 28 148 4.44e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007771 AT5G07900.1 31.967 366 211 3 265 627 69 399 1.58e-53 189
MsaG007771 AT5G07900.1 29.752 121 82 2 1 118 216 336 8.13e-12 68.2
MsaG007771 AT1G21150.3 30.390 385 220 4 246 627 11 350 2.61e-46 167
MsaG007771 AT1G21150.1 30.518 367 217 3 264 627 55 386 7.79e-46 167
MsaG007771 AT1G21150.2 30.518 367 217 3 264 627 119 450 4.91e-45 167
MsaG007771 AT1G61980.3 27.249 389 204 7 264 613 51 399 4.12e-34 135
MsaG007771 AT1G61980.2 27.249 389 204 7 264 613 51 399 4.12e-34 135
MsaG007771 AT1G61980.1 27.249 389 204 7 264 613 51 399 4.12e-34 135
MsaG007771 AT1G61990.2 26.730 419 221 8 249 627 36 408 7.14e-33 131
MsaG007771 AT1G61990.1 26.730 419 221 8 249 627 36 408 7.14e-33 131
MsaG007771 AT1G61970.2 27.179 390 207 11 264 615 51 401 1.25e-32 130
MsaG007771 AT1G61970.7 27.179 390 207 11 264 615 51 401 1.25e-32 130
MsaG007771 AT1G61970.6 27.179 390 207 11 264 615 51 401 1.25e-32 130
MsaG007771 AT1G61970.3 27.179 390 207 11 264 615 51 401 1.25e-32 130
MsaG007771 AT1G61970.5 27.179 390 207 11 264 615 51 401 1.25e-32 130
MsaG007771 AT1G61970.4 27.179 390 207 11 264 615 51 401 1.25e-32 130
MsaG007771 AT1G61970.1 27.179 390 207 11 264 615 51 401 1.25e-32 130
MsaG007771 AT1G62120.1 25.714 385 208 5 248 590 33 381 7.74e-28 117
MsaG007771 AT3G46950.1 27.273 308 179 5 238 542 23 288 2.05e-27 116
MsaG007771 AT5G64950.1 24.810 395 241 8 249 628 23 376 1.08e-24 107
MsaG007771 AT1G62150.1 23.426 397 258 10 264 619 57 448 3.78e-23 103
MsaG007771 AT1G61960.1 28.618 304 174 6 244 542 28 293 5.07e-23 103
MsaG007771 AT1G62085.1 25.945 397 233 11 265 646 55 405 1.83e-21 98.2
MsaG007771 AT1G62085.2 25.945 397 233 11 265 646 55 405 1.83e-21 98.2
MsaG007771 AT5G23930.1 26.359 368 233 10 256 590 46 408 2.13e-21 98.2
MsaG007771 AT1G62110.1 27.500 280 164 6 265 542 52 294 2.86e-19 91.7
MsaG007771 AT1G56380.2 23.989 371 240 12 266 613 28 379 9.69e-18 86.3
MsaG007771 AT1G56380.1 24.194 372 238 14 266 613 17 368 1.14e-17 85.9
MsaG007771 AT1G79220.1 21.067 356 233 7 299 644 82 399 3.52e-12 69.3

Find 133 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTGATTTCACGAATTT+AGG 0.087763 2:-22719855 None:intergenic
CATCCTTCATTGACTACTTT+TGG 0.124797 2:+22720239 MsaT007771.1:CDS
GTCAATTTGATGTCTGATTT+TGG 0.159011 2:+22716178 None:intergenic
GTCAATTTGATGTCTGATTT+TGG 0.159011 2:+22720113 MsaT007771.1:CDS
CATTTGTTACTCTGATTCTA+AGG 0.165501 2:+22718598 MsaT007771.1:CDS
CGAACAAGGCCATCTATATT+TGG 0.170334 2:+22720167 MsaT007771.1:CDS
GATTTCTGCATAGGTGTTAT+TGG 0.176606 2:-22718522 None:intergenic
ATCCTACACGCTTCATTTCT+TGG 0.196998 2:+22718360 MsaT007771.1:CDS
CAAGTTTGTTTGCTCCTTTA+AGG 0.209354 2:+22720613 MsaT007771.1:CDS
ATAATCAGATTCCTCCTTAA+AGG 0.220620 2:-22720627 None:intergenic
AATTCTAGCCGGGCTGGAGC+TGG 0.227795 2:+22718437 MsaT007771.1:CDS
TAGAGGAAGTTAAGGGTTTA+GGG 0.232380 2:+22720213 MsaT007771.1:CDS
TTAGAGGAAGTTAAGGGTTT+AGG 0.237816 2:+22720212 MsaT007771.1:CDS
AGGATTCTGGTGCGTGTTGC+GGG 0.241767 2:-22718342 None:intergenic
AGACCAGCCCCATTTGTTAA+AGG 0.263441 2:-22720321 None:intergenic
TAACAATGATATTTGTTTAC+AGG 0.264991 2:-22718644 None:intergenic
TGGCATGCCATTCACAAATT+AGG 0.295156 2:+22720703 MsaT007771.1:CDS
AGACCAACCCCATTTCTTAA+AGG 0.303126 2:-22716386 None:intergenic
ATGCCATTCTTTGTTTAATT+CGG 0.306732 2:+22720042 MsaT007771.1:CDS
CATTCTTGATCTCTTGAACT+TGG 0.316429 2:+22718685 MsaT007771.1:CDS
AACTTGATTTCACGAATTTA+GGG 0.317503 2:-22719854 None:intergenic
AAAGGATTTGATTAAGTCAT+TGG 0.323129 2:+22716258 MsaT007771.1:CDS
ATTCTAGCCGGGCTGGAGCT+GGG 0.323689 2:+22718438 MsaT007771.1:CDS
AACTTCTCAGAATATCTAAA+TGG 0.329338 2:-22720581 None:intergenic
AAGCTAGATTTCAAAATTGT+AGG 0.330980 2:-22719960 None:intergenic
ACAAAACATCTGTGGTAGTT+TGG 0.331948 2:-22720465 None:intergenic
TACAACTACCATCAACATTT+AGG 0.339838 2:-22718409 None:intergenic
AAATGAAGCGTGTAGGATTC+TGG 0.341190 2:-22718355 None:intergenic
AAGCTATTATTCAAGCATTT+CGG 0.341623 2:+22716413 MsaT007771.1:CDS
TCCTACCTCATTCACAAATT+CGG 0.344796 2:+22719588 MsaT007771.1:CDS
GATCCTTCAACGACTACTTT+TGG 0.347794 2:+22716304 MsaT007771.1:CDS
TCCTTTGAACCAAATGTAGA+TGG 0.353629 2:-22716241 None:intergenic
TGCGGTTGAGGTTGTTGATA+TGG 0.356705 2:-22719496 None:intergenic
ATTGAAACCAACATCAAATT+AGG 0.361121 2:-22720377 None:intergenic
AAGTTGATGCCTTTAAGAAA+TGG 0.366199 2:+22716377 MsaT007771.1:CDS
AGTTGATGCCTTTAAGAAAT+GGG 0.371481 2:+22716378 MsaT007771.1:CDS
TAAGTCATTAGAGGAAGTTA+AGG 0.381295 2:+22720205 MsaT007771.1:CDS
GGTGATGGCAGCTGGGTTGT+CGG 0.382719 2:+22718476 MsaT007771.1:CDS
GACATTCTTCGTATGGTTGT+AGG 0.384943 2:+22719828 MsaT007771.1:CDS
AAGAAGTTGAGAACTGAATC+TGG 0.385783 2:-22719672 None:intergenic
AGAATATTGGAGTGCGGTTG+AGG 0.397179 2:-22719508 None:intergenic
TCCTTTGAACCAAATATAGA+TGG 0.398078 2:-22720176 None:intergenic
ACAAAGAATGGCATCCTTGT+TGG 0.401490 2:-22720033 None:intergenic
TGTTTAGTCCTAAATGTTGA+TGG 0.404987 2:+22718401 MsaT007771.1:CDS
AAGTTGACACCTTTAACAAA+TGG 0.405620 2:+22720312 MsaT007771.1:CDS
ATCAGAGGAAGAAGCACCTT+TGG 0.406640 2:-22719913 None:intergenic
TTGATCTCTTGAACTTGGAC+TGG 0.416343 2:+22718690 MsaT007771.1:CDS
TGAGGAATCTTAGAGAATAT+TGG 0.425832 2:-22719521 None:intergenic
GGATTTGATTAAGTCATTAG+AGG 0.426999 2:+22720196 MsaT007771.1:CDS
AAGTCATTAGAGGAAGTTAA+GGG 0.427528 2:+22720206 MsaT007771.1:CDS
GCCATCTATATTTGGTTCAA+AGG 0.427799 2:+22720175 MsaT007771.1:CDS
AAGTCAAAGCCATCTACATT+TGG 0.428349 2:+22716232 MsaT007771.1:CDS
TTCTAGCCGGGCTGGAGCTG+GGG 0.431804 2:+22718439 MsaT007771.1:CDS
GAAATTACCTAATTTGTGAA+TGG 0.433382 2:-22720710 None:intergenic
GCCATCTACATTTGGTTCAA+AGG 0.434419 2:+22716240 MsaT007771.1:CDS
AGTTGACACCTTTAACAAAT+GGG 0.435374 2:+22720313 MsaT007771.1:CDS
CTCAACTTCTTCAAAAGTCA+TGG 0.435992 2:+22719684 MsaT007771.1:CDS
TTAGACGTGGTGATGGCAGC+TGG 0.436481 2:+22718468 MsaT007771.1:CDS
TCAATTGGGTTGGAATTCCT+TGG 0.440659 2:+22720436 MsaT007771.1:CDS
GTGATGGCAGCTGGGTTGTC+GGG 0.441940 2:+22718477 MsaT007771.1:CDS
TTGGAGGAAGTTAAGGCTCT+AGG 0.448743 2:+22716277 MsaT007771.1:CDS
GTCAGAGGTAGAAGCACCTT+TGG 0.453352 2:-22719808 None:intergenic
AGCTAGATTTCAAAATTGTA+GGG 0.454084 2:-22719959 None:intergenic
TGATCTCTTGAACTTGGACT+GGG 0.456553 2:+22718691 MsaT007771.1:CDS
TACCTCTGACATTCTTCGTA+TGG 0.462578 2:+22719821 MsaT007771.1:CDS
TAAGTCATTGGAGGAAGTTA+AGG 0.463461 2:+22716270 MsaT007771.1:CDS
TTTCATGCAATGCCCACAAA+AGG 0.467899 2:+22719757 MsaT007771.1:CDS
ATGCCGAATTAAACAAAGAA+TGG 0.473359 2:-22720045 None:intergenic
TATTTACCAAGGGTTGCGTC+TGG 0.475369 2:+22718550 MsaT007771.1:CDS
ATTATCCACAGTAAGGTGAT+AGG 0.484709 2:-22720090 None:intergenic
TAGGATTCTGGTGCGTGTTG+CGG 0.489053 2:-22718343 None:intergenic
TCTAGCCGGGCTGGAGCTGG+GGG 0.491357 2:+22718440 MsaT007771.1:CDS
TCTAAATGGTGTAACTAAGC+TGG 0.495852 2:-22720567 None:intergenic
TCCTTGCCAGACGCAACCCT+TGG 0.503866 2:-22718556 None:intergenic
TGGAGGAAGTTAAGGCTCTA+GGG 0.506417 2:+22716278 MsaT007771.1:CDS
TTTGGGTCAATCAATTGGGT+TGG 0.506924 2:+22720426 MsaT007771.1:CDS
GTTGCAGCCTAATTTGATGT+TGG 0.507199 2:+22720370 MsaT007771.1:CDS
TAACACCTATGCAGAAATCA+TGG 0.517614 2:+22718526 MsaT007771.1:CDS
ATTTGTTACTCTGATTCTAA+GGG 0.519585 2:+22718599 MsaT007771.1:CDS
AGGTGTTATTGGAAAGTCCT+AGG 0.521193 2:-22718511 None:intergenic
ACTCCAAAAGTAGTCGTTGA+AGG 0.521363 2:-22716307 None:intergenic
GTTGTAGCGGTAATTCTAGC+CGG 0.528054 2:+22718426 MsaT007771.1:CDS
AGCGGTAATTCTAGCCGGGC+TGG 0.537585 2:+22718431 MsaT007771.1:CDS
ACACCTTTAACAAATGGGGC+TGG 0.542117 2:+22720318 MsaT007771.1:CDS
AATTGCATAACTGAGGCCCT+TGG 0.542717 2:-22720512 None:intergenic
GGATTCTGGTGCGTGTTGCG+GGG 0.545947 2:-22718341 None:intergenic
GTTGCAGCCTAGTTTCATGT+TGG 0.547960 2:+22716435 MsaT007771.1:CDS
GTTCAAGAGATCAAGAATGT+TGG 0.550937 2:-22718681 None:intergenic
AATAGCCATGATTTCTGCAT+AGG 0.554457 2:-22718531 None:intergenic
ACCCCAAAAGTAGTCAATGA+AGG 0.557831 2:-22720242 None:intergenic
TTGCGTCTGGCAAGGAACTT+CGG 0.558270 2:+22718563 MsaT007771.1:CDS
ACCAAGGGTTGCGTCTGGCA+AGG 0.558361 2:+22718555 MsaT007771.1:CDS
TTGCTAGATTGCTTCGAACA+AGG 0.560619 2:+22720153 MsaT007771.1:CDS
AATTGACATTATCCACAGTA+AGG 0.560808 2:-22720097 None:intergenic
TTGTAGCGGTAATTCTAGCC+GGG 0.567506 2:+22718427 MsaT007771.1:CDS
TGGAGAAAAGGATCATTCCA+AGG 0.570897 2:+22720495 MsaT007771.1:CDS
GGATTTGATTAAGTCATTGG+AGG 0.571043 2:+22716261 MsaT007771.1:CDS
GGTCTGATTAGACGTGGTGA+TGG 0.572902 2:+22718461 MsaT007771.1:CDS
GTTGATGCCTTTAAGAAATG+GGG 0.573670 2:+22716379 MsaT007771.1:CDS
ATTGAAACCAACATGAAACT+AGG 0.577651 2:-22716442 None:intergenic
GTCGGGTTCAGCAGCTACCT+AGG 0.577884 2:+22718494 MsaT007771.1:CDS
GATTCTGGTGCGTGTTGCGG+GGG 0.580799 2:-22718340 None:intergenic
GAAATCATGGCTATTTACCA+AGG 0.581208 2:+22718539 MsaT007771.1:CDS
TGGTAGTTTGGCAAGTGCCA+AGG 0.586106 2:-22720453 None:intergenic
TGAGGTTGTTGATATGGCAA+AGG 0.590012 2:-22719490 None:intergenic
ATGCCTTTAAGAAATGGGGT+TGG 0.598819 2:+22716383 MsaT007771.1:CDS
GTTTGTTTGCTCCTTTAAGG+AGG 0.603996 2:+22720616 MsaT007771.1:CDS
AGAAACCGAATTTGTGAATG+AGG 0.604803 2:-22719593 None:intergenic
ATCAGAGTAACAAATGATGT+AGG 0.606273 2:-22718591 None:intergenic
ACCGAATTTGTGAATGAGGT+AGG 0.607992 2:-22719589 None:intergenic
AATGAAACTTGCATACACAA+TGG 0.609213 2:+22720673 MsaT007771.1:CDS
TAGACGTGGTGATGGCAGCT+GGG 0.614611 2:+22718469 MsaT007771.1:CDS
TGAATTTGAATCTGAAGCGA+TGG 0.615539 2:-22719550 None:intergenic
TAACAATGCAACAATATCAG+AGG 0.616711 2:-22719928 None:intergenic
TGAATCTGAAGCGATGGTAG+TGG 0.621211 2:-22719544 None:intergenic
AATGTTGATGGTAGTTGTAG+CGG 0.625886 2:+22718413 MsaT007771.1:CDS
CAAAAGAAATTGCATAACTG+AGG 0.634260 2:-22720519 None:intergenic
GTTGACACCTTTAACAAATG+GGG 0.635802 2:+22720314 MsaT007771.1:CDS
GCATTGCATGAAAGAAGCCA+TGG 0.636869 2:-22719747 None:intergenic
AAACTAAAACAAAACATCTG+TGG 0.637333 2:-22720473 None:intergenic
GGAGAAAAGGATCATTCCAA+GGG 0.639024 2:+22720496 MsaT007771.1:CDS
GGTATTCATAGAAAATGCAG+TGG 0.641012 2:-22720069 None:intergenic
TCTCTTGAACTTGGACTGGG+AGG 0.644353 2:+22718694 MsaT007771.1:CDS
GCATACACAATGGAAAACAA+TGG 0.644592 2:+22720683 MsaT007771.1:CDS
AAATCATGGCTATTTACCAA+GGG 0.645063 2:+22718540 MsaT007771.1:CDS
CTGAAGCGATGGTAGTGGTG+AGG 0.647119 2:-22719539 None:intergenic
TCTTAGAGAATATTGGAGTG+CGG 0.670463 2:-22719514 None:intergenic
AACCATACGAAGAATGTCAG+AGG 0.672395 2:-22719823 None:intergenic
TGCCAAGAAATGAAGCGTGT+AGG 0.673737 2:-22718362 None:intergenic
GAATACCTATCACCTTACTG+TGG 0.678196 2:+22720085 MsaT007771.1:CDS
GCATCATCAAAAGAGAACCA+TGG 0.694296 2:+22719730 MsaT007771.1:CDS
TAGGTTTCTGAAAACCAACA+AGG 0.701717 2:+22720019 MsaT007771.1:CDS
TTATCTATTAAAGCTATACG+AGG 0.742253 2:+22720646 MsaT007771.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22716187 22720724 22716187 ID=MsaG007771
Chr2 mRNA 22716187 22720724 22716187 ID=MsaT007771.1;Parent=MsaG007771
Chr2 exon 22716187 22716508 22716187 ID=MsaT007771.1.exon1;Parent=MsaT007771.1
Chr2 CDS 22716187 22716508 22716187 ID=cds.MsaT007771.1;Parent=MsaT007771.1
Chr2 exon 22718333 22718715 22718333 ID=MsaT007771.1.exon2;Parent=MsaT007771.1
Chr2 CDS 22718333 22718715 22718333 ID=cds.MsaT007771.1;Parent=MsaT007771.1
Chr2 exon 22719489 22720724 22719489 ID=MsaT007771.1.exon3;Parent=MsaT007771.1
Chr2 CDS 22719489 22720724 22719489 ID=cds.MsaT007771.1;Parent=MsaT007771.1
Gene Sequence

>MsaT007771.1

ATGTCTGATTTTGGAGTTTCTGATAATGTCATTGCTAGTATACTTAAGTCAAAGCCATCTACATTTGGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGGCTCTAGGGTTTGATCCTTCAACGACTACTTTTGGAGTTGCTTTGGTAGCCAAGAAAAGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATGCCTTTAAGAAATGGGGTTGGTCTGATGAAGCTATTATTCAAGCATTTCGGTTGCAGCCTAGTTTCATGTTGGTTTCAATTGATAAGATTGATTTGTTAATGTGTTTTTGGGTCAATCAATTAGTTTGCTTCCCCCGCAACACGCACCAGAATCCTACACGCTTCATTTCTTGGCATCCTTCTAGAGAAAATTGTTTAGTCCTAAATGTTGATGGTAGTTGTAGCGGTAATTCTAGCCGGGCTGGAGCTGGGGGTCTGATTAGACGTGGTGATGGCAGCTGGGTTGTCGGGTTCAGCAGCTACCTAGGACTTTCCAATAACACCTATGCAGAAATCATGGCTATTTACCAAGGGTTGCGTCTGGCAAGGAACTTCGGATGTACCTACATCATTTGTTACTCTGATTCTAAGGGTGTCATTGATATTTTAGCTAAGCCTGTAAACAAATATCATTGTTATGCATCTGTCATTGCCAACATTCTTGATCTCTTGAACTTGGACTGGGAGGCCTTTGCCATATCAACAACCTCAACCGCACTCCAATATTCTCTAAGATTCCTCACCACTACCATCGCTTCAGATTCAAATTCAAATTCATTCGCTGTATCCTACCTCATTCACAAATTCGGTTTCTCACATCAATTCGCACTCAAAGCTTCCAAAAATCTTCGTTTCAAAACCTCACAAAAACCAGATTCAGTTCTCAACTTCTTCAAAAGTCATGGTTTCACTGACTCAGATATACACAGCATCATCAAAAGAGAACCATGGCTTCTTTCATGCAATGCCCACAAAAGGATTCTCCCAAAGTTTCAATTTTTAATCACCAAAGGTGCTTCTACCTCTGACATTCTTCGTATGGTTGTAGGTAACCCTAAATTCGTGAAATCAAGTTTGAAGAATCATGAAACTATGTTTCATTTTTTTCTATCCAAAGGTGCTTCTTCCTCTGATATTGTTGCATTGTTAACTGCTAACCCTACAATTTTGAAATCTAGCTTGGAGAGACGAATAATCCCTCTTTTTGAATTTTTAAGTAGGTTTCTGAAAACCAACAAGGATGCCATTCTTTGTTTAATTCGGCATTCCACTGCATTTTCTATGAATACCTATCACCTTACTGTGGATAATGTCAATTTGATGTCTGATTTTGGAGTTTCTGATTCTACAATTGCTAGATTGCTTCGAACAAGGCCATCTATATTTGGTTCAAAGGATTTGATTAAGTCATTAGAGGAAGTTAAGGGTTTAGGGTTTCATCCTTCATTGACTACTTTTGGGGTTGCTTTGGTAGCCAAGCAATGTATGAGTAAAAAACTTTGGGATGAGAAAGTTGACACCTTTAACAAATGGGGCTGGTCTGATGAAGCTGTTATTCAAACATTTCAGTTGCAGCCTAATTTGATGTTGGTTTCAATTGATAAGATTGATTCGATAATGAGTTTTTGGGTCAATCAATTGGGTTGGAATTCCTTGGCACTTGCCAAACTACCACAGATGTTTTGTTTTAGTTTGGAGAAAAGGATCATTCCAAGGGCCTCAGTTATGCAATTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCCAGCTTAGTTACACCATTTAGATATTCTGAGAAGTTGTTCTTGAACAAGTTTGTTTGCTCCTTTAAGGAGGAATCTGATTATCTATTAAAGCTATACGAGGAAAAAATGAAACTTGCATACACAATGGAAAACAATGGCATGCCATTCACAAATTAG
Protein sequence

>MsaT007771.1

MSDFGVSDNVIASILKSKPSTFGSKDLIKSLEEVKALGFDPSTTTFGVALVAKKSLSKKLWDEKVDAFKKWGWSDEAIIQAFRLQPSFMLVSIDKIDLLMCFWVNQLVCFPRNTHQNPTRFISWHPSRENCLVLNVDGSCSGNSSRAGAGGLIRRGDGSWVVGFSSYLGLSNNTYAEIMAIYQGLRLARNFGCTYIICYSDSKGVIDILAKPVNKYHCYASVIANILDLLNLDWEAFAISTTSTALQYSLRFLTTTIASDSNSNSFAVSYLIHKFGFSHQFALKASKNLRFKTSQKPDSVLNFFKSHGFTDSDIHSIIKREPWLLSCNAHKRILPKFQFLITKGASTSDILRMVVGNPKFVKSSLKNHETMFHFFLSKGASSSDIVALLTANPTILKSSLERRIIPLFEFLSRFLKTNKDAILCLIRHSTAFSMNTYHLTVDNVNLMSDFGVSDSTIARLLRTRPSIFGSKDLIKSLEEVKGLGFHPSLTTFGVALVAKQCMSKKLWDEKVDTFNKWGWSDEAVIQTFQLQPNLMLVSIDKIDSIMSFWVNQLGWNSLALAKLPQMFCFSLEKRIIPRASVMQFLLMKGLRKKNASLVTPFRYSEKLFLNKFVCSFKEESDYLLKLYEEKMKLAYTMENNGMPFTN*