AlfalfaGEDB | MsaG007772

M. sativa ssp. caerulea / MsaG007772

KEGG: K08065
GO: -
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007772	KEH15995.1	97.826	138	3	0	1	138	1	138	1.66e-94	279

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007772	sp\|O82248\|NFYB5_ARATH	66.412	131	26	1	4	116	19	149	5.74e-55	172

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007772	tr\|A0A072TR10\|A0A072TR10_MEDTR	97.826	138	3	0	1	138	1	138	7.94e-95	279

TFs/TRs:

Gene ID	Type	Classification
MsaG007772	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007772	MtrunA17_Chr2g0296321	97.778	135	3	0	1	135	49	183	2.63e-95	275
MsaG007772	MtrunA17_Chr4g0067091	87.500	120	9	4	1	114	2	121	4.14e-67	202
MsaG007772	MtrunA17_Chr1g0195851	78.218	101	22	0	12	112	30	130	5.37e-57	176
MsaG007772	MtrunA17_Chr4g0076321	61.905	126	44	2	3	128	6	127	8.83e-50	155
MsaG007772	MtrunA17_Chr2g0290491	65.741	108	33	1	9	112	4	111	1.63e-48	153
MsaG007772	MtrunA17_Chr8g0384451	65.049	103	36	0	17	119	39	141	4.76e-48	154
MsaG007772	MtrunA17_Chr7g0263601	59.130	115	43	1	16	130	24	134	5.42e-48	152
MsaG007772	MtrunA17_Chr1g0191981	68.687	99	31	0	18	116	4	102	6.23e-47	148
MsaG007772	MtrunA17_Chr1g0185911	68.317	101	28	1	7	107	22	118	6.48e-47	149
MsaG007772	MtrunA17_Chr3g0102351	62.385	109	38	1	7	112	13	121	1.20e-46	149
MsaG007772	MtrunA17_Chr8g0382931	66.667	99	33	0	17	115	27	125	3.65e-46	148
MsaG007772	MtrunA17_Chr5g0446491	61.468	109	40	1	6	112	9	117	6.71e-46	147
MsaG007772	MtrunA17_Chr4g0076381	61.856	97	37	0	16	112	56	152	2.57e-43	142
MsaG007772	MtrunA17_Chr1g0165041	61.458	96	37	0	14	109	2	97	1.45e-40	134
MsaG007772	MtrunA17_Chr5g0446601	45.055	91	50	0	17	107	23	113	1.16e-24	93.6
MsaG007772	MtrunA17_Chr1g0159271	47.436	78	41	0	32	109	1	78	2.74e-21	82.4
MsaG007772	MtrunA17_Chr1g0158951	49.206	63	32	0	48	110	19	81	1.42e-16	70.5
MsaG007772	MtrunA17_Chr4g0062271	31.250	112	71	1	12	123	5	110	1.54e-16	71.6
MsaG007772	MtrunA17_Chr2g0307551	33.333	105	64	1	19	123	12	110	1.62e-16	71.6
MsaG007772	MtrunA17_Chr1g0159291	36.986	73	46	0	23	95	7	79	3.53e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007772	AT2G47810.1	66.412	131	26	1	4	116	19	149	5.83e-56	172
MsaG007772	AT4G14540.1	62.727	110	41	0	6	115	9	118	1.18e-49	156
MsaG007772	AT2G38880.2	57.812	128	48	1	7	128	10	137	1.93e-49	155
MsaG007772	AT2G38880.1	57.812	128	48	1	7	128	10	137	1.93e-49	155
MsaG007772	AT2G38880.5	57.812	128	48	1	7	128	10	137	1.93e-49	155
MsaG007772	AT2G38880.3	57.812	128	48	1	7	128	10	137	2.07e-49	155
MsaG007772	AT3G53340.5	61.739	115	40	1	16	130	27	137	1.22e-48	154
MsaG007772	AT3G53340.1	61.739	115	40	1	16	130	27	137	1.22e-48	154
MsaG007772	AT5G47640.1	60.345	116	46	0	17	132	26	141	3.11e-48	154
MsaG007772	AT1G09030.1	64.220	109	39	0	16	124	1	109	5.22e-48	151
MsaG007772	AT2G37060.2	61.404	114	36	1	6	111	10	123	6.97e-48	152
MsaG007772	AT2G37060.3	61.404	114	36	1	6	111	10	123	6.97e-48	152
MsaG007772	AT2G37060.1	61.404	114	36	1	6	111	10	123	6.97e-48	152
MsaG007772	AT2G38880.4	64.078	103	37	0	6	108	9	111	1.04e-47	150
MsaG007772	AT3G53340.3	69.474	95	29	0	16	110	27	121	1.35e-47	150
MsaG007772	AT3G53340.2	69.474	95	29	0	16	110	27	121	1.35e-47	150
MsaG007772	AT2G38880.7	65.347	101	35	0	7	107	10	110	2.36e-47	150
MsaG007772	AT2G38880.11	64.706	102	36	0	7	108	10	111	2.58e-47	150
MsaG007772	AT2G38880.8	64.706	102	36	0	7	108	10	111	2.58e-47	150
MsaG007772	AT2G13570.1	67.677	99	32	0	17	115	35	133	3.07e-47	152
MsaG007772	AT2G38880.9	64.078	103	37	0	6	108	9	111	8.07e-47	149
MsaG007772	AT2G38880.10	64.078	103	37	0	6	108	9	111	8.07e-47	149
MsaG007772	AT2G38880.6	64.078	103	37	0	6	108	9	111	8.07e-47	149
MsaG007772	AT3G53340.4	68.817	93	29	0	16	108	27	119	3.39e-46	146
MsaG007772	AT5G47670.2	56.364	110	43	1	5	109	11	120	2.40e-42	139
MsaG007772	AT5G47670.1	54.867	113	46	1	5	112	40	152	4.94e-42	139
MsaG007772	AT5G47670.3	54.867	113	46	1	5	112	40	152	4.94e-42	139
MsaG007772	AT1G21970.1	56.250	96	42	0	17	112	58	153	6.88e-38	129
MsaG007772	AT5G08190.1	33.000	100	65	1	12	109	5	104	2.41e-16	71.6
MsaG007772	AT5G08190.2	33.000	100	65	1	12	109	5	104	2.80e-16	71.2
MsaG007772	AT5G23090.4	32.039	103	68	1	12	112	5	107	1.30e-15	69.3
MsaG007772	AT5G23090.5	33.000	100	65	1	12	109	5	104	1.54e-15	69.3
MsaG007772	AT5G23090.2	33.000	100	65	1	12	109	5	104	1.54e-15	69.3
MsaG007772	AT5G23090.1	33.000	100	65	1	12	109	5	104	1.54e-15	69.3

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCCGACCAACATTGGCTAT+TGG	0.352636	2:-22742415	None:intergenic
ATGAAATTTATAGAGGTGTT+AGG	0.374658	2:+22742734	MsaT007772.1:CDS
GATACCGCCATTGTCGTTAG+AGG	0.377941	2:-22742371	None:intergenic
ACTAATAATCAAGAAATTAG+AGG	0.408687	2:+22742688	MsaT007772.1:CDS
CTTCGTAACGAGTGAAGCTT+CGG	0.427925	2:+22742522	MsaT007772.1:CDS
GAGAGAGATGAAATTTATAG+AGG	0.466053	2:+22742727	MsaT007772.1:CDS
GAATGGTGATGATATTTGTT+CGG	0.475737	2:+22742576	MsaT007772.1:CDS
CTATGCAAGTACCTTCTCAT+TGG	0.485808	2:-22742634	None:intergenic
TAACGACAATGGCGGTATCA+AGG	0.502142	2:+22742375	MsaT007772.1:CDS
GTGAAGCTTCGGAGAAATGC+AGG	0.510847	2:+22742533	MsaT007772.1:CDS
CTTCATTATCCGACCAACAT+TGG	0.513248	2:-22742422	None:intergenic
AAAATGGTTGATAACTTTGG+AGG	0.516859	2:+22742343	None:intergenic
GAAAGGAGAAAGACTGTGAA+TGG	0.518544	2:+22742559	MsaT007772.1:CDS
TAAATTTCATCTCTCTCTTG+AGG	0.531287	2:-22742721	None:intergenic
TTCGATACTTTCGCATTCTG+AGG	0.534092	2:-22742454	None:intergenic
TGTTCGGCTTTAGCAACACT+TGG	0.540056	2:+22742592	MsaT007772.1:CDS
CAATGAGAAGGTACTTGCAT+AGG	0.552859	2:+22742635	MsaT007772.1:CDS
CGGAGAAATGCAGGAAGGAA+AGG	0.555191	2:+22742542	MsaT007772.1:CDS
CAAGTACCTTCTCATTGGCT+CGG	0.563177	2:-22742629	None:intergenic
CGTTACGAAGCTTATGAACT+CGG	0.570732	2:-22742509	None:intergenic
TTGCTTCCAATAGCCAATGT+TGG	0.590887	2:+22742409	MsaT007772.1:CDS
TTCCAATAGCCAATGTTGGT+CGG	0.592047	2:+22742413	MsaT007772.1:CDS
GCGGTATCAAGGAACAAGAC+AGG	0.602800	2:+22742386	MsaT007772.1:CDS
TCAGAATGCGAAAGTATCGA+AGG	0.631763	2:+22742456	MsaT007772.1:CDS
GAAAGTATCGAAGGAAGCAA+AGG	0.632226	2:+22742465	MsaT007772.1:CDS
GGTAGTTCCTCTAACGACAA+TGG	0.647778	2:+22742364	MsaT007772.1:CDS
AATAATCAAGAAATTAGAGG+AGG	0.649585	2:+22742691	MsaT007772.1:CDS
ATTATGCCGAGCCAATGAGA+AGG	0.662962	2:+22742623	MsaT007772.1:CDS
AGCTTCGGAGAAATGCAGGA+AGG	0.709567	2:+22742537	MsaT007772.1:CDS
AGTTCCTCTAACGACAATGG+CGG	0.733992	2:+22742367	MsaT007772.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	22742346	22742762	22742346	ID=MsaG007772
Chr2	mRNA	22742346	22742762	22742346	ID=MsaT007772.1;Parent=MsaG007772
Chr2	exon	22742346	22742762	22742346	ID=MsaT007772.1.exon1;Parent=MsaT007772.1
Chr2	CDS	22742346	22742762	22742346	ID=cds.MsaT007772.1;Parent=MsaT007772.1

Gene Sequence

>MsaT007772.1

ATGGTTGATAACTTTGGAGGTAGTTCCTCTAACGACAATGGCGGTATCAAGGAACAAGACAGGTTGCTTCCAATAGCCAATGTTGGTCGGATAATGAAGCAGATTTTGCCTCAGAATGCGAAAGTATCGAAGGAAGCAAAGGAAACAATGCAAGAATGTGTGTCCGAGTTCATAAGCTTCGTAACGAGTGAAGCTTCGGAGAAATGCAGGAAGGAAAGGAGAAAGACTGTGAATGGTGATGATATTTGTTCGGCTTTAGCAACACTTGGTTTTGATGATTATGCCGAGCCAATGAGAAGGTACTTGCATAGGTATAGAGAGTTAGAAGTTGATAAAATTAATACTAATAATCAAGAAATTAGAGGAGGAAATAGTCCTCAAGAGAGAGATGAAATTTATAGAGGTGTTAGGCTGTAA

Protein sequence

>MsaT007772.1

MVDNFGGSSSNDNGGIKEQDRLLPIANVGRIMKQILPQNAKVSKEAKETMQECVSEFISFVTSEASEKCRKERRKTVNGDDICSALATLGFDDYAEPMRRYLHRYRELEVDKINTNNQEIRGGNSPQERDEIYRGVRL*