AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007782

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007782 MtrunA17_Chr2g0296421 95.494 1043 46 1 1 1043 1 1042 0.0 2031
MsaG007782 MtrunA17_Chr8g0352361 80.326 798 137 8 250 1043 112 893 0.0 1287
MsaG007782 MtrunA17_Chr8g0352361 51.534 163 54 5 1 157 12 155 7.56e-34 140
MsaG007782 MtrunA17_Chr1g0191141 31.062 763 407 21 340 1002 315 1058 5.53e-94 323
MsaG007782 MtrunA17_Chr7g0224051 31.457 693 388 19 390 1002 387 1072 1.74e-88 308
MsaG007782 MtrunA17_Chr3g0114161 31.304 690 376 21 399 1002 526 1203 4.30e-87 306
MsaG007782 MtrunA17_Chr7g0225791 31.277 697 357 20 388 1002 584 1240 3.07e-86 303
MsaG007782 MtrunA17_Chr4g0047081 30.482 643 344 17 433 1002 578 1190 3.15e-81 288
MsaG007782 MtrunA17_Chr5g0437621 29.343 685 400 19 395 1002 582 1259 3.24e-80 286
MsaG007782 MtrunA17_Chr7g0233021 31.562 640 348 22 433 1002 567 1186 2.38e-78 280
MsaG007782 MtrunA17_Chr7g0232971 30.294 680 372 21 402 1002 596 1252 2.61e-78 280
MsaG007782 MtrunA17_Chr7g0233031 28.424 679 382 20 402 1002 547 1199 8.17e-72 261
MsaG007782 MtrunA17_Chr7g0232991 29.443 557 308 11 514 1002 75 614 1.32e-66 237
MsaG007782 MtrunA17_Chr7g0233001 32.525 495 272 15 550 1002 1 475 1.98e-66 233
MsaG007782 MtrunA17_Chr2g0320781 39.407 236 126 9 763 988 5 233 1.42e-35 135
MsaG007782 MtrunA17_Chr3g0137091 24.805 512 320 14 512 1000 289 758 6.67e-32 135
MsaG007782 MtrunA17_Chr4g0052341 25.486 514 306 17 514 1001 747 1209 1.65e-29 127
MsaG007782 MtrunA17_Chr5g0394201 25.773 485 314 16 508 985 359 804 8.94e-29 124
MsaG007782 MtrunA17_Chr5g0393461 24.725 546 335 18 500 1000 172 686 1.21e-28 124
MsaG007782 MtrunA17_Chr8g0384531 25.328 533 326 16 505 994 377 880 4.48e-28 122
MsaG007782 MtrunA17_Chr5g0442141 25.824 546 322 19 509 1027 614 1103 1.75e-27 121
MsaG007782 MtrunA17_Chr1g0190391 27.907 215 141 4 506 707 576 789 3.19e-26 116
MsaG007782 MtrunA17_Chr1g0205891 25.309 486 316 17 508 985 372 818 3.34e-26 116
MsaG007782 MtrunA17_Chr1g0163011 23.870 553 354 17 492 994 175 710 5.09e-26 115
MsaG007782 MtrunA17_Chr3g0095091 25.049 515 309 18 509 997 568 1031 2.18e-25 114
MsaG007782 MtrunA17_Chr5g0403921 24.486 486 328 14 509 984 181 637 9.61e-24 108
MsaG007782 MtrunA17_Chr1g0183681 23.992 496 321 14 533 998 410 879 4.19e-23 106
MsaG007782 MtrunA17_Chr4g0066651 24.000 550 332 21 508 1023 985 1482 3.01e-21 100
MsaG007782 MtrunA17_Chr4g0040301 36.364 176 96 4 850 1016 381 549 1.18e-19 94.7
MsaG007782 MtrunA17_Chr7g0248031 25.743 505 295 20 508 979 990 1447 3.78e-18 90.9
MsaG007782 MtrunA17_Chr1g0190381 34.177 158 95 2 850 998 189 346 1.41e-17 87.4
MsaG007782 MtrunA17_Chr7g0232121 22.792 566 341 24 512 1034 39 551 1.78e-17 88.2
MsaG007782 MtrunA17_Chr1g0175191 31.351 185 109 4 811 985 1017 1193 9.03e-16 82.8
MsaG007782 MtrunA17_Chr1g0168031 33.333 144 91 2 852 995 990 1128 5.14e-13 73.6
MsaG007782 MtrunA17_Chr2g0281601 37.398 123 72 2 863 985 1146 1263 2.84e-12 71.2
MsaG007782 MtrunA17_Chr7g0224021 35.632 87 43 2 506 581 25 109 1.04e-11 63.2
MsaG007782 MtrunA17_Chr1g0212331 33.858 127 78 3 861 986 880 1001 2.26e-11 68.2
MsaG007782 MtrunA17_Chr2g0320751 30.667 150 97 3 847 995 848 991 2.40e-11 68.2
MsaG007782 MtrunA17_Chr7g0255821 33.113 151 92 4 840 986 639 784 2.90e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007782 AT2G16390.2 57.004 821 273 16 244 1043 130 891 0.0 904
MsaG007782 AT2G16390.1 56.968 818 278 15 244 1043 127 888 0.0 904
MsaG007782 AT2G21450.1 54.478 670 282 5 394 1043 148 814 0.0 752
MsaG007782 AT2G21450.2 54.478 670 282 5 394 1043 155 821 0.0 752
MsaG007782 AT2G21450.3 54.478 670 282 5 394 1043 57 723 0.0 752
MsaG007782 AT1G05490.1 30.540 704 378 22 385 1002 675 1353 2.10e-90 317
MsaG007782 AT3G42670.4 28.404 683 405 14 395 1002 539 1212 2.46e-81 289
MsaG007782 AT3G42670.2 28.341 681 406 14 395 1002 539 1210 6.92e-81 288
MsaG007782 AT3G42670.3 28.341 681 406 14 395 1002 539 1210 6.92e-81 288
MsaG007782 AT3G42670.1 28.341 681 406 14 395 1002 539 1210 6.92e-81 288
MsaG007782 AT3G24340.1 31.644 651 378 15 397 1002 453 1081 3.08e-80 285
MsaG007782 AT5G20420.1 29.587 703 411 20 390 1019 542 1233 7.11e-79 282
MsaG007782 AT3G32330.1 37.283 346 175 4 490 826 1 313 2.99e-64 221
MsaG007782 AT3G32280.1 35.870 276 128 2 431 706 248 474 1.58e-50 186
MsaG007782 AT1G08600.4 26.598 579 322 17 506 998 712 1273 3.22e-45 178
MsaG007782 AT1G08600.3 26.598 579 322 17 506 998 712 1273 3.22e-45 178
MsaG007782 AT1G08600.2 26.598 579 322 17 506 998 712 1273 3.22e-45 178
MsaG007782 AT1G08600.1 26.170 577 306 18 506 998 712 1252 2.06e-38 156
MsaG007782 AT3G31900.1 32.734 278 127 5 431 708 153 370 6.18e-38 147
MsaG007782 AT5G63950.1 25.796 597 347 20 464 1006 330 884 6.11e-29 125
MsaG007782 AT5G44800.1 25.144 521 321 16 515 1014 692 1164 6.23e-29 126
MsaG007782 AT4G31900.3 25.094 530 319 16 512 1023 225 694 8.80e-29 125
MsaG007782 AT4G31900.2 25.094 530 319 16 512 1023 184 653 9.44e-29 125
MsaG007782 AT4G31900.1 25.094 530 319 16 512 1023 225 694 1.12e-28 125
MsaG007782 AT5G63950.2 24.958 589 345 19 464 1006 330 867 4.46e-27 119
MsaG007782 AT2G13370.1 24.522 575 326 19 464 986 554 1072 2.39e-26 117
MsaG007782 AT2G13370.2 24.522 575 326 19 464 986 554 1072 2.39e-26 117
MsaG007782 AT2G13370.3 24.522 575 326 19 464 986 554 1072 2.39e-26 117
MsaG007782 AT2G25170.1 24.394 578 359 20 462 1000 204 742 4.28e-26 117
MsaG007782 AT2G25170.4 24.394 578 359 20 462 1000 204 742 4.28e-26 117
MsaG007782 AT3G06010.1 25.361 485 315 17 509 985 400 845 4.60e-26 116
MsaG007782 AT2G25170.2 24.394 578 359 20 462 1000 221 759 5.76e-26 116
MsaG007782 AT2G25170.3 24.394 578 359 20 462 1000 230 768 8.78e-26 115
MsaG007782 AT5G19310.2 25.615 488 311 17 508 985 118 563 9.59e-25 111
MsaG007782 AT5G19310.1 25.615 488 311 17 508 985 382 827 2.02e-24 111
MsaG007782 AT2G28290.3 24.905 526 332 18 509 1011 751 1236 6.49e-23 107
MsaG007782 AT2G28290.4 24.905 526 332 18 509 1011 751 1236 6.49e-23 107
MsaG007782 AT2G28290.6 24.905 526 332 18 509 1011 752 1237 6.60e-23 107
MsaG007782 AT2G28290.2 24.905 526 332 18 509 1011 751 1236 6.94e-23 106
MsaG007782 AT2G28290.5 24.905 526 332 18 509 1011 752 1237 7.25e-23 106
MsaG007782 AT2G28290.1 24.905 526 332 18 509 1011 751 1236 7.56e-23 106
MsaG007782 AT2G18760.4 24.405 504 307 17 533 998 404 871 4.76e-22 103
MsaG007782 AT2G18760.1 24.405 504 307 17 533 998 404 871 4.76e-22 103
MsaG007782 AT2G18760.2 24.405 504 307 17 533 998 404 871 4.76e-22 103
MsaG007782 AT2G18760.7 24.405 504 307 17 533 998 404 871 4.76e-22 103
MsaG007782 AT2G18760.5 24.405 504 307 17 533 998 404 871 4.76e-22 103
MsaG007782 AT2G18760.6 24.405 504 307 17 533 998 404 871 4.76e-22 103
MsaG007782 AT2G18760.3 24.405 504 307 17 533 998 463 930 7.24e-22 103
MsaG007782 AT1G03750.1 36.943 157 94 2 844 1000 523 674 6.53e-20 96.3
MsaG007782 AT2G02090.1 21.828 536 352 17 512 997 213 731 6.66e-19 92.8
MsaG007782 AT5G66750.1 25.137 549 348 20 509 1042 199 699 8.27e-19 92.8
MsaG007782 AT2G46020.5 25.502 498 304 17 508 979 977 1433 1.71e-18 92.4
MsaG007782 AT2G46020.1 25.502 498 304 17 508 979 977 1433 1.71e-18 92.4
MsaG007782 AT2G46020.6 25.502 498 304 17 508 979 977 1433 1.71e-18 92.4
MsaG007782 AT2G46020.2 24.752 505 300 15 508 979 977 1434 2.95e-18 91.7
MsaG007782 AT2G46020.3 24.752 505 300 15 508 979 977 1434 2.95e-18 91.7
MsaG007782 AT2G46020.4 24.752 505 300 15 508 979 977 1434 2.95e-18 91.7
MsaG007782 AT3G19210.3 29.680 219 128 5 498 704 166 370 6.09e-18 89.7
MsaG007782 AT3G19210.3 26.531 147 102 2 855 1000 541 682 7.45e-11 67.0
MsaG007782 AT3G19210.2 29.680 219 128 5 498 704 166 370 6.17e-18 90.1
MsaG007782 AT3G19210.2 26.531 147 102 2 855 1000 539 680 4.15e-11 67.8
MsaG007782 AT3G19210.1 29.680 219 128 5 498 704 166 370 6.18e-18 90.1
MsaG007782 AT3G19210.1 26.531 147 102 2 855 1000 541 682 3.60e-11 68.2
MsaG007782 AT3G12810.1 29.577 213 126 4 847 1043 1073 1277 9.20e-16 83.6
MsaG007782 AT3G12810.2 29.577 213 126 4 847 1043 1069 1273 9.27e-16 83.6
MsaG007782 AT5G43530.1 37.121 132 78 2 864 995 1126 1252 1.77e-15 82.4
MsaG007782 AT2G44980.1 24.390 492 312 22 512 985 51 500 3.04e-15 81.3
MsaG007782 AT1G61140.1 33.566 143 90 1 864 1006 1122 1259 1.08e-12 73.2
MsaG007782 AT1G61140.3 33.813 139 87 1 864 1002 964 1097 1.24e-12 72.8
MsaG007782 AT1G11100.5 36.066 122 73 2 864 985 927 1043 1.72e-12 72.4
MsaG007782 AT1G11100.3 36.066 122 73 2 864 985 971 1087 1.73e-12 72.4
MsaG007782 AT1G11100.1 36.066 122 73 2 864 985 1070 1186 1.94e-12 72.4
MsaG007782 AT1G11100.2 36.066 122 73 2 864 985 1113 1229 2.03e-12 72.4
MsaG007782 AT1G11100.4 36.066 122 73 2 864 985 1114 1230 2.03e-12 72.4
MsaG007782 AT3G20010.3 33.871 124 67 2 867 985 628 741 5.16e-11 67.4
MsaG007782 AT3G20010.2 33.871 124 67 2 867 985 603 716 5.52e-11 67.4
MsaG007782 AT3G20010.1 33.600 125 68 2 867 986 894 1008 8.11e-11 67.0
MsaG007782 AT3G20010.5 33.600 125 68 2 867 986 894 1008 8.11e-11 67.0
MsaG007782 AT3G20010.7 33.600 125 68 2 867 986 894 1008 8.11e-11 67.0

Find 199 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTGCCTCTAAAATATT+TGG 0.125276 2:+22844782 MsaT007782.1:CDS
CGCTTAGAGAGTGGAAAATT+TGG 0.193521 2:+22839963 MsaT007782.1:CDS
TCTTTCTTTGTGTTATCTTT+AGG 0.226098 2:-22841964 None:intergenic
TGAAGTCATCATCATTGATT+TGG 0.260279 2:+22840460 MsaT007782.1:CDS
TCTGCAATTGGTTTCAAGTC+TGG 0.267700 2:-22844609 None:intergenic
ATGGAAAATAAGAATGTTAC+TGG 0.277957 2:+22840119 MsaT007782.1:CDS
ACATGAGGAAGAAGATAATA+AGG 0.284394 2:+22842008 MsaT007782.1:CDS
TCATCTGAATCAATGATAAA+TGG 0.290481 2:-22840239 None:intergenic
ATGTAAAAGCCCTGTATAAA+AGG 0.296271 2:+22845267 MsaT007782.1:CDS
ACATCATAATGTCATAGATT+TGG 0.300000 2:+22840148 MsaT007782.1:CDS
TTTCTAGAAAGTCCATTGTT+AGG 0.301488 2:+22845239 MsaT007782.1:CDS
GTTTCCATCTTTATAAACTT+TGG 0.310104 2:-22844270 None:intergenic
AGTCTTTCACACCAGGTAAA+TGG 0.310452 2:-22844329 None:intergenic
GCAGGAGACTGAACTGGTTT+TGG 0.312654 2:-22840731 None:intergenic
CTTGTATTGGAACTCAAATA+TGG 0.319708 2:-22843092 None:intergenic
TTTCCATCTTTATAAACTTT+GGG 0.324220 2:-22844269 None:intergenic
GTACTGTGATCTTCCTCTTC+TGG 0.324552 2:-22845107 None:intergenic
TCTTTCTTTGTGTTATCTTC+AGG 0.325344 2:-22842051 None:intergenic
CTGCAATTGGTTTCAAGTCT+GGG 0.326657 2:-22844608 None:intergenic
TTTCGAAAATGTGGTTTGAA+TGG 0.333393 2:+22845150 MsaT007782.1:CDS
AATACTATCGACTTGGAAAA+AGG 0.333771 2:+22843876 MsaT007782.1:CDS
ATTACAGTTATCACAGATAA+TGG 0.340357 2:-22844043 None:intergenic
TCTGTTACTCGTCAAGCTAT+TGG 0.341013 2:+22845020 MsaT007782.1:CDS
CTCCTAATGCGTTGGACAAT+AGG 0.350767 2:-22844300 None:intergenic
GGTTGAAGCAGGGCGTTTGC+TGG 0.358007 2:-22838699 None:intergenic
TGTGAAAGACTTCTGTGATT+TGG 0.367803 2:+22844341 MsaT007782.1:CDS
ATTCAAGTGAGGAAGATGAT+AGG 0.373070 2:+22840375 MsaT007782.1:CDS
GGCAATGCAACTTCATGAAA+TGG 0.373763 2:-22840710 None:intergenic
CTGGCTCATGCTCCAGGATC+TGG 0.375281 2:+22843769 MsaT007782.1:CDS
ATAGTAGTGCAGTACCTTGC+TGG 0.375718 2:-22844436 None:intergenic
GTTTCTTAAGCAATATATCT+TGG 0.378503 2:-22844086 None:intergenic
GCACACCGAAAAGGATGTTT+TGG 0.378651 2:+22840184 MsaT007782.1:CDS
AGCTGTTATACATGATTTGC+GGG 0.378708 2:+22844407 MsaT007782.1:CDS
TCACTACATTTCTCTGCAAT+TGG 0.379021 2:-22844621 None:intergenic
AATGTTATCATTGATTCAAA+TGG 0.380062 2:+22840656 MsaT007782.1:CDS
ACCCAGAAAATGCAAATATT+AGG 0.380882 2:+22840017 MsaT007782.1:CDS
GTGAGGGTATATCACTGGTT+GGG 0.382550 2:+22844958 MsaT007782.1:CDS
ATTCAGTTGAGGAAGATGAT+AGG 0.385380 2:+22840573 MsaT007782.1:CDS
ATCAGCTGGTGAGAAGCTTT+TGG 0.391575 2:+22844746 MsaT007782.1:CDS
CCGCCCAAAGTTTATAAAGA+TGG 0.396049 2:+22844266 MsaT007782.1:CDS
TGCATTCTGGCTCATGCTCC+AGG 0.404134 2:+22843763 MsaT007782.1:CDS
ATGTTATCATTGATTCAAAT+GGG 0.404166 2:+22840657 MsaT007782.1:CDS
TGGAAAGGTTAGCTATGAAA+TGG 0.408305 2:+22844802 MsaT007782.1:CDS
ATGACGGGCTAGAAGATGTT+TGG 0.408645 2:+22842130 MsaT007782.1:CDS
TTCTAGAAAGTCCATTGTTA+GGG 0.412455 2:+22845240 MsaT007782.1:CDS
GTTATGTTTGTCGCGTTTGT+GGG 0.414777 2:+22843047 MsaT007782.1:CDS
GTTTCTTCATCATGATCTGT+TGG 0.434415 2:-22840296 None:intergenic
TCACCCCGTCTCTCACATCT+TGG 0.439166 2:-22844686 None:intergenic
AGGAATTAATTTCGAAAATG+TGG 0.440301 2:+22845141 MsaT007782.1:CDS
ATCTTCCTCACTTGAATCAA+TGG 0.440751 2:-22840369 None:intergenic
ATGAATGTCTTTCCAGATCC+TGG 0.441533 2:-22843781 None:intergenic
AACCTATTGTCCAACGCATT+AGG 0.441584 2:+22844298 MsaT007782.1:CDS
TTTGGATGAGGAAGATGATA+AGG 0.441721 2:+22840478 MsaT007782.1:CDS
TCATCTAAAATGAGAATTGA+CGG 0.441909 2:-22844108 None:intergenic
ACCTCTCGTGGTGCTTCCTA+AGG 0.442095 2:+22843852 MsaT007782.1:CDS
ACCTGGTGGGAGACCATCCT+GGG 0.448352 2:+22843737 MsaT007782.1:CDS
AAATGGAAGGGCTGGAGTCT+TGG 0.448957 2:+22844819 MsaT007782.1:CDS
TCTAATGCAGGAGACTGAAC+TGG 0.453819 2:-22840737 None:intergenic
GGTTATGTTTGTCGCGTTTG+TGG 0.457173 2:+22843046 MsaT007782.1:CDS
ACCACGAGAGGTCGTGCATC+GGG 0.459021 2:-22843841 None:intergenic
AGCAAACGCCCTGCTTCAAC+CGG 0.460097 2:+22838701 MsaT007782.1:CDS
CATATTTGAGTTCCAATACA+AGG 0.465416 2:+22843093 MsaT007782.1:CDS
AAAGACAAAGGTAATTATGT+TGG 0.466178 2:+22841982 MsaT007782.1:CDS
ACCAGTGTCTAACCTTGTAT+TGG 0.466307 2:-22843105 None:intergenic
AACCTGGTGGGAGACCATCC+TGG 0.468533 2:+22843736 MsaT007782.1:CDS
TAATGTCATAGATTTGGATT+CGG 0.469260 2:+22840154 MsaT007782.1:CDS
TTCTCCTCCATGGTAAGCAT+TGG 0.471176 2:-22841908 None:intergenic
ATGAAGTTAAGACAATAAAG+AGG 0.472373 2:+22844535 MsaT007782.1:CDS
CAAGTCGATAGTATTCCCTT+AGG 0.474320 2:-22843868 None:intergenic
AAGGTACTGCACTACTATAA+AGG 0.474619 2:+22844441 MsaT007782.1:CDS
TTCAGATGAGGAGGATGATA+AGG 0.476090 2:+22840253 MsaT007782.1:CDS
AAACACTTTGCAGAAGGATC+CGG 0.477672 2:+22844368 MsaT007782.1:CDS
CCCCAGGATGGTCTCCCACC+AGG 0.479756 2:-22843738 None:intergenic
CTTTCCGACCAGGCCAGAAA+AGG 0.480032 2:+22845048 MsaT007782.1:CDS
CTTACAATCATTTCTAATGC+AGG 0.480541 2:-22840749 None:intergenic
ATTGGCCGTGCTTTCCGACC+AGG 0.480741 2:+22845038 MsaT007782.1:CDS
AATGTAATACCGGTTGAAGC+AGG 0.482687 2:-22838710 None:intergenic
AAAGCTTCTCACCAGCTGAT+TGG 0.482830 2:-22844743 None:intergenic
TAGCTATGAAATGGAAGGGC+TGG 0.483976 2:+22844811 MsaT007782.1:CDS
TGACAGTAGATCACAACAAT+TGG 0.483985 2:+22843957 MsaT007782.1:CDS
AGGATCTGGAAAGACATTCA+TGG 0.485077 2:+22843783 MsaT007782.1:CDS
CTAAGGGAATACTATCGACT+TGG 0.486814 2:+22843869 MsaT007782.1:CDS
GAACTTATATGTCTGAATCA+TGG 0.492636 2:+22843575 MsaT007782.1:CDS
TGCCTCTAAAATATTTGGAA+AGG 0.493636 2:+22844787 MsaT007782.1:CDS
ACTGTTGGTATCCTAAGAAA+AGG 0.496655 2:-22844014 None:intergenic
GATCTGCATGTTCTCCTCCA+TGG 0.504352 2:-22841918 None:intergenic
ATGATGATGACTTCATGAAA+TGG 0.505154 2:-22840452 None:intergenic
TTCATTTGATTTGCATGTCG+AGG 0.507539 2:-22843676 None:intergenic
TCTCCTCCATGGTAAGCATT+GGG 0.510319 2:-22841907 None:intergenic
ATAACATTTGAAATTGCATA+CGG 0.510461 2:-22839936 None:intergenic
AAAGACAAAGGTCTTTATGT+TGG 0.511523 2:+22842069 MsaT007782.1:CDS
CCAGGAATTTCGCCTGAAGA+AGG 0.513792 2:+22839840 MsaT007782.1:intron
TCATAGATTTGGATTCGGAC+GGG 0.516289 2:+22840159 MsaT007782.1:CDS
TTCAGTTGAGGAAGATGATA+GGG 0.520994 2:+22840574 MsaT007782.1:CDS
GACGAGTAACAGACGGGTTG+AGG 0.521054 2:-22845010 None:intergenic
TAGATGAGGGACACACTCCT+AGG 0.521780 2:+22844124 MsaT007782.1:CDS
CGAACCAAGATGTGAGAGAC+GGG 0.522137 2:+22844682 MsaT007782.1:CDS
TAGCTGTTATACATGATTTG+CGG 0.523136 2:+22844406 MsaT007782.1:CDS
GGTGAGGGTATATCACTGGT+TGG 0.524182 2:+22844957 MsaT007782.1:CDS
TGAGGGTATATCACTGGTTG+GGG 0.525743 2:+22844959 MsaT007782.1:CDS
ATGACTTCATGAAATGGTGC+TGG 0.526184 2:-22840446 None:intergenic
GAGGGAGATGGCAATGGCAA+TGG 0.526773 2:+22842152 MsaT007782.1:CDS
CATAGATTTGGATTCGGACG+GGG 0.528458 2:+22840160 MsaT007782.1:CDS
TTATAAACTTTGGGCGGACA+AGG 0.528879 2:-22844260 None:intergenic
GGAGCACTCCCTTTGTTCAG+TGG 0.530923 2:-22844874 None:intergenic
TTCAAGTGAGGAAGATGATA+GGG 0.531455 2:+22840376 MsaT007782.1:CDS
CATCATCATTGATTTGGATG+AGG 0.532601 2:+22840466 MsaT007782.1:CDS
GGTTGAAAACACTTTGCAGA+AGG 0.533466 2:+22844362 MsaT007782.1:CDS
AGAATCGTCCACTGAACAAA+GGG 0.535424 2:+22844866 MsaT007782.1:CDS
TTGGTTTCAAGTCTGGGTGC+AGG 0.536173 2:-22844602 None:intergenic
GAGGAGGATGATAAGGATCA+TGG 0.536967 2:+22840260 MsaT007782.1:CDS
GTCATAGATTTGGATTCGGA+CGG 0.540389 2:+22840158 MsaT007782.1:CDS
GTATCAGTATTCTCGTTCCT+AGG 0.541700 2:-22844141 None:intergenic
GGACGGGGAGCACACCGAAA+AGG 0.543559 2:+22840175 MsaT007782.1:CDS
CACCACGAGAGGTCGTGCAT+CGG 0.550292 2:-22843842 None:intergenic
TCACAGTACTTGTGTCAAGA+AGG 0.552998 2:+22845121 MsaT007782.1:CDS
GGATCCTACTCCTAATGCGT+TGG 0.553435 2:-22844308 None:intergenic
GTTATAAATCGCTTAGAGAG+TGG 0.555195 2:+22839954 MsaT007782.1:CDS
TTCTATATCTGAGCATACAA+TGG 0.558790 2:+22844647 MsaT007782.1:CDS
TGAACAAAGGGAGTGCTCCA+TGG 0.559236 2:+22844878 MsaT007782.1:CDS
GATGATAGGGATCATGATGA+AGG 0.559405 2:+22840587 MsaT007782.1:CDS
AGCTGCTGATTCTCCAGAAG+AGG 0.560162 2:+22845094 MsaT007782.1:CDS
AGTTGAGAGAGTTGATGTGA+AGG 0.561498 2:+22845202 MsaT007782.1:CDS
TATCATTGATTCAGATGAGG+AGG 0.561761 2:+22840244 MsaT007782.1:CDS
TCTGGCCTGGTCGGAAAGCA+CGG 0.562735 2:-22845043 None:intergenic
AAATCATCCACCCGAAGAAG+AGG 0.565897 2:+22842976 MsaT007782.1:CDS
TTTACATCTTCCCCTAACAA+TGG 0.567105 2:-22845251 None:intergenic
CATGATCCCAATGCTTACCA+TGG 0.571021 2:+22841901 MsaT007782.1:CDS
ACAGTAAAATCAACTAGGCC+AGG 0.571652 2:-22844483 None:intergenic
TGAAGCAGGGCGTTTGCTGG+AGG 0.574648 2:-22838696 None:intergenic
CCTCTCGTGGTGCTTCCTAA+GGG 0.575239 2:+22843853 MsaT007782.1:CDS
AATAGTTGATTAGCAAGTCT+AGG 0.577621 2:-22840059 None:intergenic
ACGGGCTAGAAGATGTTTGG+AGG 0.582292 2:+22842133 MsaT007782.1:CDS
TCAATCAAAGCATGTGGTGA+GGG 0.583869 2:+22844942 MsaT007782.1:CDS
CTGAATCTATGCCAATCAGC+TGG 0.587383 2:+22844732 MsaT007782.1:CDS
GGAGTTTCTGACATGGCAAG+TGG 0.588515 2:+22843897 MsaT007782.1:CDS
TTCGGGTCAATCAAAGCATG+TGG 0.589622 2:+22844936 MsaT007782.1:CDS
CGGGCTAGAAGATGTTTGGA+GGG 0.589781 2:+22842134 MsaT007782.1:CDS
TGTTATCATCGATTCAGTTG+AGG 0.590425 2:+22840562 MsaT007782.1:CDS
GAAAATTGAATCCTTCGACT+TGG 0.592086 2:-22843705 None:intergenic
AGAAGATGTTTGGAGGGAGA+TGG 0.593018 2:+22842140 MsaT007782.1:CDS
AAGGTTAGCTATGAAATGGA+AGG 0.595000 2:+22844806 MsaT007782.1:CDS
ATGTAATACCGGTTGAAGCA+GGG 0.596547 2:-22838709 None:intergenic
TCCCGATGCACGACCTCTCG+TGG 0.597869 2:+22843840 MsaT007782.1:CDS
TTGTCCAACGCATTAGGAGT+AGG 0.597875 2:+22844304 MsaT007782.1:CDS
TAGGAACGAGAATACTGATA+CGG 0.598994 2:+22844143 MsaT007782.1:CDS
GAGAATCGTCCACTGAACAA+AGG 0.601522 2:+22844865 MsaT007782.1:CDS
GTCAATCAAAGCATGTGGTG+AGG 0.601636 2:+22844941 MsaT007782.1:CDS
ATGAAACCGCACCAAGTCGA+AGG 0.602444 2:+22843694 MsaT007782.1:CDS
AGATAATGGACGAAAACTGT+TGG 0.602446 2:-22844029 None:intergenic
AGGAGTAGGATCCATTTACC+TGG 0.603942 2:+22844318 MsaT007782.1:CDS
TGAAGTTAAGACAATAAAGA+GGG 0.605026 2:+22844536 MsaT007782.1:CDS
ATTTATCATTGATTCAGATG+AGG 0.606144 2:+22840241 MsaT007782.1:CDS
TTGAATCCTTCGACTTGGTG+CGG 0.606513 2:-22843700 None:intergenic
GGAAGTCTTAAAACAGTGGG+TGG 0.608781 2:+22843978 MsaT007782.1:CDS
AAGGTTGATACTGAAGATGA+CGG 0.609380 2:+22842114 MsaT007782.1:CDS
TTTATATCAAAATCATGTCA+AGG 0.609653 2:+22844218 MsaT007782.1:CDS
CGCTTGCCATGTTTAACACA+TGG 0.610807 2:+22839891 MsaT007782.1:CDS
AGGTTGATACTGAAGATGAC+GGG 0.612267 2:+22842115 MsaT007782.1:CDS
TAATTATGTTGGAGTACATG+AGG 0.612315 2:+22841993 MsaT007782.1:CDS
ATTGGAAGTCTTAAAACAGT+GGG 0.613903 2:+22843975 MsaT007782.1:CDS
AAGTTCAAGATGTCTTCTGT+AGG 0.616210 2:+22844567 MsaT007782.1:CDS
GTTTGTGGGGTTATTGACAG+AGG 0.616734 2:+22843061 MsaT007782.1:CDS
GCGAACCAAGATGTGAGAGA+CGG 0.616830 2:+22844681 MsaT007782.1:CDS
TGATGAAGGAACTCCCAACA+TGG 0.619010 2:+22840601 MsaT007782.1:CDS
TGTTATCATTGATTCAAATG+GGG 0.619334 2:+22840658 MsaT007782.1:CDS
ATGTGGTGAGGGTATATCAC+TGG 0.620246 2:+22844953 MsaT007782.1:CDS
AGAGTTGATGTGAAGGAATG+TGG 0.620738 2:+22845209 MsaT007782.1:CDS
TGATGAAGAAACTCCCAACA+TGG 0.622428 2:+22840100 MsaT007782.1:CDS
TGATGAAGAAACTCCCAACA+TGG 0.622428 2:+22840505 MsaT007782.1:CDS
TTTGAATGGAATGAATATTG+TGG 0.623573 2:+22845164 MsaT007782.1:CDS
CCATCTTTATAAACTTTGGG+CGG 0.625396 2:-22844266 None:intergenic
GCACCACAGTAAAATCAACT+AGG 0.627117 2:-22844488 None:intergenic
TGTTACCATTGATTCAAGTG+AGG 0.629258 2:+22840364 MsaT007782.1:CDS
TAGCTTGACGAGTAACAGAC+GGG 0.631651 2:-22845016 None:intergenic
TCACAGAAGTCTTTCACACC+AGG 0.631873 2:-22844336 None:intergenic
TGCTAATCAACTATTGAGAG+TGG 0.634052 2:+22840067 MsaT007782.1:CDS
TCTTTATGTTGGTGTACGTG+AGG 0.635289 2:+22842080 MsaT007782.1:CDS
GATAACACAAAGAAAGACAA+AGG 0.636337 2:+22841970 MsaT007782.1:CDS
GATAACACAAAGAAAGACAA+AGG 0.636337 2:+22842057 MsaT007782.1:CDS
TTTCTTGTCAGAAACCTGGT+GGG 0.638542 2:+22843724 MsaT007782.1:CDS
ACATCACCATGTGTTAAACA+TGG 0.639224 2:-22839897 None:intergenic
CCCTTAGGAAGCACCACGAG+AGG 0.643968 2:-22843853 None:intergenic
AATTGGAAGTCTTAAAACAG+TGG 0.644070 2:+22843974 MsaT007782.1:CDS
ATAGCTTGACGAGTAACAGA+CGG 0.650423 2:-22845017 None:intergenic
AGGTTAGCTATGAAATGGAA+GGG 0.651392 2:+22844807 MsaT007782.1:CDS
GATCCCAATGCTTACCATGG+AGG 0.651668 2:+22841904 MsaT007782.1:CDS
TGTTTGGAGGGAGATGGCAA+TGG 0.662485 2:+22842146 MsaT007782.1:CDS
TTATGTTTGTCGCGTTTGTG+GGG 0.662905 2:+22843048 MsaT007782.1:CDS
CCTGGTGGGAGACCATCCTG+GGG 0.668528 2:+22843738 MsaT007782.1:CDS
ACGTGAGGAAGAAGATAACA+AGG 0.674639 2:+22842095 MsaT007782.1:CDS
ACTTCATGAAATGGTGCTGG+AGG 0.676126 2:-22840443 None:intergenic
TGGTGCTCAACATGACACCC+AGG 0.688712 2:+22844505 MsaT007782.1:CDS
AAAATTCAACAATTCACCCG+AGG 0.695204 2:+22844902 MsaT007782.1:CDS
TCTAGAAAGTCCATTGTTAG+GGG 0.705989 2:+22845241 MsaT007782.1:CDS
CTGGTGGGAGACCATCCTGG+GGG 0.719970 2:+22843739 MsaT007782.1:CDS
TTTGCGGGAGATGACCAGCA+AGG 0.730371 2:+22844422 MsaT007782.1:CDS
GAACCAAGATGTGAGAGACG+GGG 0.738263 2:+22844683 MsaT007782.1:CDS
ATATCAAAATCATGTCAAGG+AGG 0.742826 2:+22844221 MsaT007782.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22838689 22845386 22838689 ID=MsaG007782
Chr2 mRNA 22838689 22845386 22838689 ID=MsaT007782.1;Parent=MsaG007782
Chr2 exon 22838689 22838722 22838689 ID=MsaT007782.1.exon1;Parent=MsaT007782.1
Chr2 CDS 22838689 22838722 22838689 ID=cds.MsaT007782.1;Parent=MsaT007782.1
Chr2 exon 22839844 22840766 22839844 ID=MsaT007782.1.exon2;Parent=MsaT007782.1
Chr2 CDS 22839844 22840766 22839844 ID=cds.MsaT007782.1;Parent=MsaT007782.1
Chr2 exon 22841895 22842185 22841895 ID=MsaT007782.1.exon3;Parent=MsaT007782.1
Chr2 CDS 22841895 22842185 22841895 ID=cds.MsaT007782.1;Parent=MsaT007782.1
Chr2 exon 22842965 22843114 22842965 ID=MsaT007782.1.exon4;Parent=MsaT007782.1
Chr2 CDS 22842965 22843114 22842965 ID=cds.MsaT007782.1;Parent=MsaT007782.1
Chr2 exon 22843559 22845288 22843559 ID=MsaT007782.1.exon5;Parent=MsaT007782.1
Chr2 CDS 22843559 22845288 22843559 ID=cds.MsaT007782.1;Parent=MsaT007782.1
Chr2 exon 22845383 22845386 22845383 ID=MsaT007782.1.exon6;Parent=MsaT007782.1
Chr2 CDS 22845383 22845386 22845383 ID=cds.MsaT007782.1;Parent=MsaT007782.1
Gene Sequence

>MsaT007782.1

ATGGCGTCCTCCAGCAAACGCCCTGCTTCAACCGGAATTTCGCCTGAAGAAGGACACAAAAAACTTAAACTGTCAAGTGATCGCTTGCCATGTTTAACACATGGTGATGTTGTAGATTACAGCAGTCCGTATGCAATTTCAAATGTTATAAATCGCTTAGAGAGTGGAAAATTTGGAAGTGTGACAAAAAATATTAAAGACCTTATAACCCAGAAAATGCAAATATTAGGCCCTTACTTTGCAAAATGTCCTAGACTTGCTAATCAACTATTGAGAGTGGTGACAGATCATGATGAAGAAACTCCCAACATGGAAAATAAGAATGTTACTGGTTTGCAACATCATAATGTCATAGATTTGGATTCGGACGGGGAGCACACCGAAAAGGATGTTTTGGCACCAGAAAAAGGATATAATGTTACTGCAGTACCATTTATCATTGATTCAGATGAGGAGGATGATAAGGATCATGGTCATGATACTGTTCCAACAGATCATGATGAAGAAACTCCCTACATTGAAAGTGAAAAAGCTCCTGGTATCTCCCATCAAAATGTTACCATTGATTCAAGTGAGGAAGATGATAGGGATCAAAAAGAGGATGTTTTGGCACCAAGAAGAGAAAAAGATGTTCCTCCAGCACCATTTCATGAAGTCATCATCATTGATTTGGATGAGGAAGATGATAAGGATCATGATGAAGAAACTCCCAACATGGAAAATGAAAAAGTTACTCCTATGTCCTATCAAAATGTTATCATCGATTCAGTTGAGGAAGATGATAGGGATCATGATGAAGGAACTCCCAACATGGAAAATGAAAAAGTTACCGGGATGTCCTATCAAAATGTTATCATTGATTCAAATGGGGAAGATGACAAAGATGAAACTTCTGTTCTTCCATTTCATGAAGTTGCATTGCCAAAACCAGTTCAGTCTCCTGCATTAGAAATGATTGGATATCATGATCCCAATGCTTACCATGGAGGAGAACATGCAGATCTAAAATTTGAAACAAGTCTGCTTCCTAAAGATAACACAAAGAAAGACAAAGGTAATTATGTTGGAGTACATGAGGAAGAAGATAATAAGGAATTTGAAACAAGTCTGTCTCCTGAAGATAACACAAAGAAAGACAAAGGTCTTTATGTTGGTGTACGTGAGGAAGAAGATAACAAGGTTGATACTGAAGATGACGGGCTAGAAGATGTTTGGAGGGAGATGGCAATGGCAATGGAAACTTCTAAAGATGTTTCTGCAAATCATCCACCCGAAGAAGAGGAAAATGACGCTGACTGTGATCATTCTTTCATTTTAAAAGACGATCTTGGTTATGTTTGTCGCGTTTGTGGGGTTATTGACAGAGGAATTGAAACCATATTTGAGTTCCAATACAAGGTTAAAAAGAGCACAAGAACTTATATGTCTGAATCATGGAATGCCCAAGAAAAAGCTGATATATTTGGAGTTAAGATTGCTAAAGACGATCTCTCAGTAACTGAAATATCAGCACATCCTCGACATGCAAATCAAATGAAACCGCACCAAGTCGAAGGATTCAATTTTCTTGTCAGAAACCTGGTGGGAGACCATCCTGGGGGATGCATTCTGGCTCATGCTCCAGGATCTGGAAAGACATTCATGGTTATCTGTTTCATACAGAGTTTTCTAGAGAAGTATCCCGATGCACGACCTCTCGTGGTGCTTCCTAAGGGAATACTATCGACTTGGAAAAAGGAGTTTCTGACATGGCAAGTGGAAGATGTACCACTTTATGATTTTTACAGTGTGAAGGCTGACAGTAGATCACAACAATTGGAAGTCTTAAAACAGTGGGTGGATAACAAGAGTATCCTTTTCTTAGGATACCAACAGTTTTCGTCCATTATCTGTGATAACTGTAATAACAATACATCAATATCTTGCCAAGATATATTGCTTAAGAAACCGTCAATTCTCATTTTAGATGAGGGACACACTCCTAGGAACGAGAATACTGATACGGTACAGTCCCTCGCAAAAGTACAAACACCAAGAAAAGTTGTTTTGTCCGGGACTTTATATCAAAATCATGTCAAGGAGGTATTTAACGTGCTTAACCTTGTCCGCCCAAAGTTTATAAAGATGGAAACATCTAAACCTATTGTCCAACGCATTAGGAGTAGGATCCATTTACCTGGTGTGAAAGACTTCTGTGATTTGGTTGAAAACACTTTGCAGAAGGATCCGGATTTTAAAAGGAAAGTAGCTGTTATACATGATTTGCGGGAGATGACCAGCAAGGTACTGCACTACTATAAAGGAGATTTTCTTGATGAGCTTCCTGGCCTAGTTGATTTTACTGTGGTGCTCAACATGACACCCAGGCAAAAGCATGAAGTTAAGACAATAAAGAGGGTATTTAGAAAGTTCAAGATGTCTTCTGTAGGTAGTGCTGTATACCTGCACCCAGACTTGAAACCAATTGCAGAGAAATGTAGTGAAAATTCTATATCTGAGCATACAATGGATGATCTAATAGCGAACCAAGATGTGAGAGACGGGGTGAAATTAAAATTCTTTCGCAATATGCTGAATCTATGCCAATCAGCTGGTGAGAAGCTTTTGGTGTTCAGTCAATATCTCTTGCCTCTAAAATATTTGGAAAGGTTAGCTATGAAATGGAAGGGCTGGAGTCTTGGCAAAGAAATTTTTGTAATATCAGGAGAATCGTCCACTGAACAAAGGGAGTGCTCCATGGAAAAATTCAACAATTCACCCGAGGCCAAAATCTTTTTCGGGTCAATCAAAGCATGTGGTGAGGGTATATCACTGGTTGGGGCATCTCGTGTCATCATCTTAGATGTGCACCTCAACCCGTCTGTTACTCGTCAAGCTATTGGCCGTGCTTTCCGACCAGGCCAGAAAAGGAAAGTGTTTGTGTATCGATTGATAGCTGCTGATTCTCCAGAAGAGGAAGATCACAGTACTTGTGTCAAGAAGGAATTAATTTCGAAAATGTGGTTTGAATGGAATGAATATTGTGGTGACAAAGCTTTCGAAGTTGAGAGAGTTGATGTGAAGGAATGTGGTGATATGTTTCTAGAAAGTCCATTGTTAGGGGAAGATGTAAAAGCCCTGTATAAAAGGTAA
Protein sequence

>MsaT007782.1

MASSSKRPASTGISPEEGHKKLKLSSDRLPCLTHGDVVDYSSPYAISNVINRLESGKFGSVTKNIKDLITQKMQILGPYFAKCPRLANQLLRVVTDHDEETPNMENKNVTGLQHHNVIDLDSDGEHTEKDVLAPEKGYNVTAVPFIIDSDEEDDKDHGHDTVPTDHDEETPYIESEKAPGISHQNVTIDSSEEDDRDQKEDVLAPRREKDVPPAPFHEVIIIDLDEEDDKDHDEETPNMENEKVTPMSYQNVIIDSVEEDDRDHDEGTPNMENEKVTGMSYQNVIIDSNGEDDKDETSVLPFHEVALPKPVQSPALEMIGYHDPNAYHGGEHADLKFETSLLPKDNTKKDKGNYVGVHEEEDNKEFETSLSPEDNTKKDKGLYVGVREEEDNKVDTEDDGLEDVWREMAMAMETSKDVSANHPPEEEENDADCDHSFILKDDLGYVCRVCGVIDRGIETIFEFQYKVKKSTRTYMSESWNAQEKADIFGVKIAKDDLSVTEISAHPRHANQMKPHQVEGFNFLVRNLVGDHPGGCILAHAPGSGKTFMVICFIQSFLEKYPDARPLVVLPKGILSTWKKEFLTWQVEDVPLYDFYSVKADSRSQQLEVLKQWVDNKSILFLGYQQFSSIICDNCNNNTSISCQDILLKKPSILILDEGHTPRNENTDTVQSLAKVQTPRKVVLSGTLYQNHVKEVFNVLNLVRPKFIKMETSKPIVQRIRSRIHLPGVKDFCDLVENTLQKDPDFKRKVAVIHDLREMTSKVLHYYKGDFLDELPGLVDFTVVLNMTPRQKHEVKTIKRVFRKFKMSSVGSAVYLHPDLKPIAEKCSENSISEHTMDDLIANQDVRDGVKLKFFRNMLNLCQSAGEKLLVFSQYLLPLKYLERLAMKWKGWSLGKEIFVISGESSTEQRECSMEKFNNSPEAKIFFGSIKACGEGISLVGASRVIILDVHLNPSVTRQAIGRAFRPGQKRKVFVYRLIAADSPEEEDHSTCVKKELISKMWFEWNEYCGDKAFEVERVDVKECGDMFLESPLLGEDVKALYKR*