AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007795


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007795 MtrunA17_Chr2g0296291 74.658 292 72 1 4 293 119 410 8.42e-153 434
MsaG007795 MtrunA17_Chr2g0296251 77.737 274 59 1 10 281 125 398 7.45e-149 423
MsaG007795 MtrunA17_Chr2g0296711 75.352 284 65 2 16 294 116 399 7.18e-148 421
MsaG007795 MtrunA17_Chr2g0296221 76.364 275 61 2 24 294 153 427 3.03e-145 415
MsaG007795 MtrunA17_Chr2g0296231 71.831 284 77 1 16 296 112 395 2.86e-143 409
MsaG007795 MtrunA17_Chr2g0296171 76.336 262 58 2 24 281 134 395 6.43e-135 388
MsaG007795 MtrunA17_Chr2g0315731 71.273 275 77 1 24 296 144 418 4.22e-134 386
MsaG007795 MtrunA17_Chr2g0296741 67.133 286 89 2 16 296 133 418 6.31e-132 381
MsaG007795 MtrunA17_Chr2g0296721 70.545 275 78 2 24 296 124 397 6.91e-131 377
MsaG007795 MtrunA17_Chr2g0296191 74.131 259 63 2 24 278 736 994 1.04e-124 380
MsaG007795 MtrunA17_Chr2g0296191 64.130 276 93 3 8 280 237 509 2.85e-110 342
MsaG007795 MtrunA17_Chr2g0296181 67.018 285 62 4 16 295 114 371 1.65e-119 348
MsaG007795 MtrunA17_Chr2g0296551 81.910 199 34 1 100 296 1 199 8.66e-114 326
MsaG007795 MtrunA17_Chr2g0296151 53.676 272 121 2 24 293 114 382 1.32e-97 292
MsaG007795 MtrunA17_Chr2g0295741 54.044 272 120 2 24 293 120 388 5.60e-96 288
MsaG007795 MtrunA17_Chr2g0296111 54.945 273 109 4 24 289 113 378 4.04e-94 283
MsaG007795 MtrunA17_Chr2g0296211 66.990 206 63 2 95 295 167 372 5.56e-91 275
MsaG007795 MtrunA17_Chr2g0295731 52.273 264 121 3 24 285 121 381 1.42e-89 271
MsaG007795 MtrunA17_Chr2g0296701 78.235 170 35 1 129 296 2 171 1.60e-89 264
MsaG007795 MtrunA17_Chr2g0296121 50.000 272 131 2 24 293 200 468 2.56e-87 268
MsaG007795 MtrunA17_Chr4g0067121 48.881 268 132 3 24 289 124 388 7.34e-84 257
MsaG007795 MtrunA17_Chr2g0296301 45.725 269 139 4 24 288 108 373 2.71e-77 240
MsaG007795 MtrunA17_Chr2g0296751 79.167 144 27 2 155 296 1 143 5.35e-76 229
MsaG007795 MtrunA17_Chr2g0295761 48.473 262 129 3 24 282 132 390 1.38e-70 223
MsaG007795 MtrunA17_Chr4g0067671 45.306 245 131 1 24 268 116 357 1.03e-66 212
MsaG007795 MtrunA17_Chr2g0296131 59.524 168 62 2 130 293 9 174 2.01e-64 200
MsaG007795 MtrunA17_Chr4g0067141 44.186 258 139 3 24 279 116 370 5.29e-64 206
MsaG007795 MtrunA17_Chr2g0296161 54.971 171 75 1 126 294 1 171 2.03e-62 195
MsaG007795 MtrunA17_Chr2g0286191 45.318 267 127 7 24 281 103 359 7.66e-61 197
MsaG007795 MtrunA17_Chr2g0295721 61.947 113 43 0 126 238 1 113 2.66e-45 149
MsaG007795 MtrunA17_Chr2g0286221 51.282 156 72 3 128 281 16 169 4.30e-41 140
MsaG007795 MtrunA17_Chr8g0377801 30.000 260 176 3 24 279 128 385 4.98e-28 111
MsaG007795 MtrunA17_Chr4g0001831 31.600 250 159 6 24 266 109 353 1.76e-27 109
MsaG007795 MtrunA17_Chr8g0377781 30.078 256 173 4 24 275 115 368 1.09e-24 102
MsaG007795 MtrunA17_Chr4g0067131 50.617 81 38 1 199 277 1 81 6.25e-20 82.4
MsaG007795 MtrunA17_Chr4g0067681 50.000 62 31 0 188 249 1 62 4.72e-14 65.9
MsaG007795 MtrunA17_Chr2g0295711 50.746 67 32 1 24 90 120 185 8.06e-14 68.9
MsaG007795 MtrunA17_Chr2g0295751 58.182 55 23 0 24 78 121 175 5.07e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007795 AT5G07900.1 33.077 260 169 3 24 278 140 399 2.14e-39 142
MsaG007795 AT1G21150.3 30.153 262 179 3 11 268 77 338 1.05e-29 115
MsaG007795 AT1G21150.1 30.153 262 179 3 11 268 113 374 1.79e-29 115
MsaG007795 AT1G21150.2 30.153 262 179 3 11 268 177 438 4.19e-29 115
MsaG007795 AT5G64950.1 27.907 258 168 4 24 268 110 362 2.13e-24 102
MsaG007795 AT1G61990.2 26.667 285 160 6 24 269 123 397 4.38e-20 90.1
MsaG007795 AT1G61990.1 26.667 285 160 6 24 269 123 397 4.38e-20 90.1
MsaG007795 AT1G61980.3 26.897 290 168 7 24 274 123 407 2.33e-18 85.1
MsaG007795 AT1G61980.2 26.897 290 168 7 24 274 123 407 2.33e-18 85.1
MsaG007795 AT1G61980.1 26.897 290 168 7 24 274 123 407 2.33e-18 85.1
MsaG007795 AT1G61970.2 25.442 283 165 8 24 266 123 399 3.72e-17 81.6
MsaG007795 AT1G61970.7 25.442 283 165 8 24 266 123 399 3.72e-17 81.6
MsaG007795 AT1G61970.6 25.442 283 165 8 24 266 123 399 3.72e-17 81.6
MsaG007795 AT1G61970.3 25.442 283 165 8 24 266 123 399 3.72e-17 81.6
MsaG007795 AT1G61970.5 25.442 283 165 8 24 266 123 399 3.72e-17 81.6
MsaG007795 AT1G61970.4 25.442 283 165 8 24 266 123 399 3.72e-17 81.6
MsaG007795 AT1G61970.1 25.442 283 165 8 24 266 123 399 3.72e-17 81.6
MsaG007795 AT1G62120.1 26.370 292 171 6 24 275 127 414 1.15e-15 77.4
MsaG007795 AT1G62110.1 29.282 181 120 3 19 195 118 294 2.08e-12 67.8
MsaG007795 AT3G46950.1 28.729 181 121 4 19 198 118 291 9.92e-12 65.5
MsaG007795 AT1G62150.1 30.000 170 111 3 30 195 135 300 3.56e-11 63.9

Find 55 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTTGGTGGCTGATTT+TGG 0.113704 2:-23058037 MsaT007795.1:CDS
GCCATCTATATTTGGTTTAA+AGG 0.141804 2:-23057975 MsaT007795.1:CDS
CAGTCAAGGCCATCTATATT+TGG 0.179813 2:-23057983 MsaT007795.1:CDS
GATCCTTCGTTGACTACTTT+TGG 0.192467 2:-23057911 MsaT007795.1:CDS
TTGGAGGAAGTTAAGGGTTT+AGG 0.192505 2:-23057938 MsaT007795.1:CDS
TGGAGGAAGTTAAGGGTTTA+GGG 0.236533 2:-23057937 MsaT007795.1:CDS
AAACTATAACAAAACATATT+TGG 0.239296 2:+23057677 None:intergenic
TATTGTGGCTAATATCAATT+TGG 0.259534 2:-23058050 MsaT007795.1:CDS
AGACCAACCCCATTTCTTAA+AGG 0.307244 2:+23057829 None:intergenic
TTTGTTATAGTTTACATAAA+AGG 0.315414 2:-23057667 MsaT007795.1:CDS
GCAGAGAATGGTGTCCTTGC+TGG 0.321424 2:+23058117 None:intergenic
CTTGAGCAAGTTTGTCTTGA+AGG 0.325723 2:-23057543 MsaT007795.1:CDS
TCTCTGCTTAATTCGATATT+CGG 0.329319 2:-23058101 MsaT007795.1:CDS
AAAGGATTTGATTAAGTCAT+TGG 0.335353 2:-23057957 MsaT007795.1:CDS
AAACTAATCTGTAAAATTGC+AGG 0.337554 2:+23058190 None:intergenic
TGTAGGTAACCCTAAGTTCT+TGG 0.343348 2:-23058305 MsaT007795.1:intron
AAGCTTAATTCCAAGAACTT+AGG 0.347303 2:+23058295 None:intergenic
AAGTTGATACCTTTAAGAAA+TGG 0.365182 2:-23057838 MsaT007795.1:CDS
AGTTGATACCTTTAAGAAAT+GGG 0.365271 2:-23057837 MsaT007795.1:CDS
ATTGAAGCCAACATCAAATT+AGG 0.374513 2:+23057773 None:intergenic
ATATCTTCAGAATAAGTAAA+TGG 0.375368 2:+23057569 None:intergenic
TAAGTCATTGGAGGAAGTTA+AGG 0.377755 2:-23057945 MsaT007795.1:CDS
AATTGATATTAGCCACAATA+AGG 0.390705 2:+23058053 None:intergenic
AGCTTAATTCCAAGAACTTA+GGG 0.393617 2:+23058296 None:intergenic
ATCAGATGAAGAAGCGCCTT+TGG 0.403170 2:+23058237 None:intergenic
TCCTTTAAACCAAATATAGA+TGG 0.404648 2:+23057974 None:intergenic
TTACATAAAAGGATCATTCC+AGG 0.411963 2:-23057656 MsaT007795.1:CDS
TCAATTAGGTTGGGATTCCT+TGG 0.416390 2:-23057714 MsaT007795.1:CDS
TTGGGTCAATCAATTAGGTT+GGG 0.436055 2:-23057723 MsaT007795.1:CDS
AAGTCATTGGAGGAAGTTAA+GGG 0.441441 2:-23057944 MsaT007795.1:CDS
AATTGCAAAACTGAGGCTCC+TGG 0.473795 2:+23057638 None:intergenic
GAAATCACATATTAAGTGAA+TGG 0.476870 2:+23057446 None:intergenic
TTTGGGTCAATCAATTAGGT+TGG 0.477232 2:-23057724 MsaT007795.1:CDS
GTTGCTTTGGTAGCCAAGAA+AGG 0.483813 2:-23057887 MsaT007795.1:CDS
AACTAATCTGTAAAATTGCA+GGG 0.522503 2:+23058191 None:intergenic
ATATCAGCCTAATTTGATGT+TGG 0.524802 2:-23057780 MsaT007795.1:CDS
ATTAGCCACAATAAGGCGAT+AGG 0.536156 2:+23058060 None:intergenic
TTGCTAGTTTGCTTCAGTCA+AGG 0.547608 2:-23057997 MsaT007795.1:CDS
TGTGGCTAATATCAATTTGG+TGG 0.549748 2:-23058047 MsaT007795.1:CDS
TGGAAATTTAGCAAGTGCCA+AGG 0.550727 2:+23057697 None:intergenic
GTTGATACCTTTAAGAAATG+GGG 0.553181 2:-23057836 MsaT007795.1:CDS
GGATTTGATTAAGTCATTGG+AGG 0.561173 2:-23057954 MsaT007795.1:CDS
TGCGCCGCTGAACTCTCCAT+TGG 0.570549 2:-23059694 MsaT007795.1:intron
ATATCGAATTAAGCAGAGAA+TGG 0.572002 2:+23058105 None:intergenic
AAATCCCTATCGCCTTATTG+TGG 0.576235 2:-23058065 MsaT007795.1:CDS
ATACCTTTAAGAAATGGGGT+TGG 0.587688 2:-23057832 MsaT007795.1:CDS
GAGCAAGTTTGTCTTGAAGG+AGG 0.590871 2:-23057540 MsaT007795.1:CDS
TAGGTTCTTTAATTCCAGCA+AGG 0.604223 2:-23058131 MsaT007795.1:CDS
TTAGCCACAATAAGGCGATA+GGG 0.609256 2:+23058061 None:intergenic
CAAAAGAAATTGCAAAACTG+AGG 0.625549 2:+23057631 None:intergenic
GTTACCAATGGAGAGTTCAG+CGG 0.658433 2:+23059690 None:intergenic
AATGAAACTTGCATACACAA+AGG 0.663379 2:-23057483 MsaT007795.1:CDS
CTATCTATTAAAGTTATACG+AGG 0.674399 2:-23057510 MsaT007795.1:CDS
ACCCCAAAAGTAGTCAACGA+AGG 0.684823 2:+23057908 None:intergenic
GCATACACAAAGGAGAACAA+TGG 0.692068 2:-23057473 MsaT007795.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 23057457 23059719 23057457 ID=MsaG007795
Chr2 mRNA 23057457 23059719 23057457 ID=MsaT007795.1;Parent=MsaG007795
Chr2 exon 23057457 23058322 23057457 ID=MsaT007795.1.exon2;Parent=MsaT007795.1
Chr2 CDS 23057457 23058322 23057457 ID=cds.MsaT007795.1;Parent=MsaT007795.1
Chr2 exon 23059695 23059719 23059695 ID=MsaT007795.1.exon1;Parent=MsaT007795.1
Chr2 CDS 23059695 23059719 23059695 ID=cds.MsaT007795.1;Parent=MsaT007795.1
Gene Sequence

>MsaT007795.1

ATGTGCGCCGCTGAACTCTCCATTGGTAACCCTAAGTTCTTGGAATTAAGCTTGAAGAATCATCAAATTAAGTTTGAATATTTTCTATCCAAAGGCGCTTCTTCATCTGATATTGTTTCATTGTTAACTGCAAACCCTGCAATTTTACAGATTAGTTTGGAGAAACGAATAATCCCTCTTTTTGAATTGTTAAATAGGTTCTTTAATTCCAGCAAGGACACCATTCTCTGCTTAATTCGATATTCGGCTGCATTTATTATAAATCCCTATCGCCTTATTGTGGCTAATATCAATTTGGTGGCTGATTTTGGAGTTTCTGATAATGTCATTGCTAGTTTGCTTCAGTCAAGGCCATCTATATTTGGTTTAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGGGTTTAGGGTTTGATCCTTCGTTGACTACTTTTGGGGTTGCTTTGGTAGCCAAGAAAGGTTTAAGTAAAAAACTTTGGGATGAGAAAGTTGATACCTTTAAGAAATGGGGTTGGTCTGATGAAGATGTAATTCGAGCATTTAGATATCAGCCTAATTTGATGTTGGCTTCAATTGATAAGATTAGTTTGCTGATGAGTTTTTGGGTCAATCAATTAGGTTGGGATTCCTTGGCACTTGCTAAATTTCCAAATATGTTTTGTTATAGTTTACATAAAAGGATCATTCCAGGAGCCTCAGTTTTGCAATTTCTTTTGATGAAAGGATTGCGAAAAAAGAATGCAAGCTTAGTCACACCATTTACTTATTCTGAAGATATGTTCTTGAGCAAGTTTGTCTTGAAGGAGGAGTCTAACTATCTATTAAAGTTATACGAGGAAAAAATGAAACTTGCATACACAAAGGAGAACAATGGCATGCCATTCACTTAA

Protein sequence

>MsaT007795.1

MCAAELSIGNPKFLELSLKNHQIKFEYFLSKGASSSDIVSLLTANPAILQISLEKRIIPLFELLNRFFNSSKDTILCLIRYSAAFIINPYRLIVANINLVADFGVSDNVIASLLQSRPSIFGLKDLIKSLEEVKGLGFDPSLTTFGVALVAKKGLSKKLWDEKVDTFKKWGWSDEDVIRAFRYQPNLMLASIDKISLLMSFWVNQLGWDSLALAKFPNMFCYSLHKRIIPGASVLQFLLMKGLRKKNASLVTPFTYSEDMFLSKFVLKEESNYLLKLYEEKMKLAYTKENNGMPFT*