AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007820 MtrunA17_Chr2g0296251 78.680 197 42 0 5 201 139 335 1.78e-108 317
MsaG007820 MtrunA17_Chr2g0296711 75.622 201 49 0 1 201 123 323 7.16e-105 309
MsaG007820 MtrunA17_Chr2g0296231 71.642 201 57 0 1 201 119 319 6.97e-103 303
MsaG007820 MtrunA17_Chr2g0296221 70.792 202 57 1 2 201 150 351 5.80e-96 286
MsaG007820 MtrunA17_Chr2g0315731 70.500 200 59 0 2 201 141 340 5.84e-96 286
MsaG007820 MtrunA17_Chr2g0296741 68.159 201 64 0 1 201 140 340 9.74e-95 283
MsaG007820 MtrunA17_Chr2g0296171 70.792 202 57 1 2 201 131 332 6.20e-94 280
MsaG007820 MtrunA17_Chr2g0296291 67.677 198 64 0 6 203 140 337 2.49e-91 274
MsaG007820 MtrunA17_Chr2g0296721 69.154 201 59 2 2 201 121 319 2.67e-91 273
MsaG007820 MtrunA17_Chr2g0296191 71.144 201 56 1 3 201 734 934 7.08e-91 287
MsaG007820 MtrunA17_Chr2g0296191 63.500 200 70 1 2 201 251 447 3.14e-80 258
MsaG007820 MtrunA17_Chr2g0296181 74.033 181 46 1 1 181 121 300 1.37e-89 269
MsaG007820 MtrunA17_Chr2g0296151 52.970 202 92 1 2 203 111 309 1.60e-72 225
MsaG007820 MtrunA17_Chr2g0296111 54.327 208 90 2 2 207 110 314 3.43e-71 221
MsaG007820 MtrunA17_Chr2g0296121 51.777 197 92 1 2 198 197 390 2.60e-67 214
MsaG007820 MtrunA17_Chr2g0295731 51.777 197 92 1 2 198 118 311 4.07e-66 208
MsaG007820 MtrunA17_Chr2g0295741 49.246 199 98 1 2 200 117 312 1.89e-63 202
MsaG007820 MtrunA17_Chr2g0296551 74.380 121 31 0 81 201 1 121 5.03e-60 187
MsaG007820 MtrunA17_Chr4g0067121 43.350 203 112 1 1 203 120 319 2.91e-59 191
MsaG007820 MtrunA17_Chr2g0296301 45.411 207 98 3 2 201 105 303 2.62e-56 183
MsaG007820 MtrunA17_Chr4g0067671 47.500 200 102 1 2 201 113 309 1.40e-54 178
MsaG007820 MtrunA17_Chr2g0295761 48.259 201 100 3 2 201 129 326 6.42e-54 177
MsaG007820 MtrunA17_Chr4g0067141 45.503 189 100 1 2 190 113 298 3.18e-51 170
MsaG007820 MtrunA17_Chr2g0296211 70.370 108 32 0 93 200 187 294 1.66e-46 157
MsaG007820 MtrunA17_Chr2g0296701 78.261 92 20 0 110 201 2 93 3.74e-46 150
MsaG007820 MtrunA17_Chr2g0286191 40.777 206 112 5 2 203 100 299 8.52e-42 145
MsaG007820 MtrunA17_Chr2g0295721 64.130 92 33 0 107 198 1 92 1.03e-36 125
MsaG007820 MtrunA17_Chr2g0296161 51.327 113 55 0 107 219 1 113 2.47e-35 123
MsaG007820 MtrunA17_Chr2g0296131 60.417 96 38 0 106 201 4 99 9.24e-35 121
MsaG007820 MtrunA17_Chr2g0296751 74.242 66 17 0 136 201 1 66 8.05e-30 108
MsaG007820 MtrunA17_Chr4g0001831 31.683 202 130 5 2 198 106 304 2.18e-24 99.0
MsaG007820 MtrunA17_Chr2g0286221 47.917 96 47 2 109 203 16 109 1.15e-21 88.2
MsaG007820 MtrunA17_Chr8g0377781 27.184 206 144 4 2 205 112 313 3.22e-16 76.3
MsaG007820 MtrunA17_Chr8g0377801 25.123 203 148 3 2 202 125 325 8.72e-15 72.4
MsaG007820 MtrunA17_Chr2g0295711 50.794 63 31 0 2 64 117 179 3.52e-13 65.9
MsaG007820 MtrunA17_Chr2g0295751 55.172 58 26 0 2 59 118 175 2.96e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007820 AT5G07900.1 34.158 202 128 4 1 198 136 336 3.38e-31 118
MsaG007820 AT1G21150.1 27.919 197 140 1 4 198 126 322 1.21e-21 92.0
MsaG007820 AT1G21150.3 27.919 197 140 1 4 198 90 286 1.27e-21 91.7
MsaG007820 AT1G21150.2 27.919 197 140 1 4 198 190 386 1.60e-21 92.4
MsaG007820 AT5G64950.1 25.743 202 146 2 1 198 106 307 1.24e-17 80.9
MsaG007820 AT1G62150.1 27.841 176 125 2 2 176 126 300 1.02e-12 67.0
MsaG007820 AT3G46950.1 27.374 179 122 2 2 179 120 291 3.66e-12 65.1
MsaG007820 AT1G61980.3 28.804 184 111 5 2 176 120 292 7.77e-12 64.3
MsaG007820 AT1G61980.2 28.804 184 111 5 2 176 120 292 7.77e-12 64.3
MsaG007820 AT1G61980.1 28.804 184 111 5 2 176 120 292 7.77e-12 64.3
MsaG007820 AT1G61960.1 28.249 177 124 3 2 177 120 294 2.07e-11 63.2
MsaG007820 AT1G62120.1 27.684 177 126 2 2 177 124 299 3.07e-11 62.4

Find 45 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTTGATGACTGATTT+TGG 0.089088 2:-23279447 MsaT007820.1:CDS
TTAATTTGGTGATGAGATTT+TGG 0.155421 2:-23279152 MsaT007820.1:CDS
GATCCTTCAATCACTACTTT+TGG 0.192080 2:-23279321 MsaT007820.1:CDS
AAACTACTTTGTAAAATTCT+AGG 0.193354 2:+23279600 None:intergenic
TTCAATTGATAAGATTAATT+TGG 0.211307 2:-23279166 MsaT007820.1:CDS
TCAATTGGGTTGGGATTCTT+TGG 0.261496 2:-23279124 MsaT007820.1:CDS
TTGGAGGAAGTCAAGTGTTT+AGG 0.277234 2:-23279348 MsaT007820.1:CDS
TTTGTTATAGTTTACATAAA+AGG 0.290343 2:-23279078 MsaT007820.1:CDS
ATTGAAGTCAACATCAAATT+AGG 0.302705 2:+23279183 None:intergenic
AATTGATATTAGCCACAATT+AGG 0.333706 2:+23279463 None:intergenic
AACTGATTTGATTAAGTCAT+TGG 0.334181 2:-23279367 MsaT007820.1:CDS
ATTTGATGTCTTTAAGAAAT+GGG 0.334222 2:-23279247 MsaT007820.1:CDS
AAACTGTTATTCGAGTATTT+AGG 0.335264 2:-23279212 MsaT007820.1:CDS
TGGAGGAAGTCAAGTGTTTA+GGG 0.339402 2:-23279347 MsaT007820.1:CDS
AATTTGATGTCTTTAAGAAA+TGG 0.359065 2:-23279248 MsaT007820.1:CDS
CACTCAAGGCCACGTATGTT+TGG 0.362140 2:-23279393 MsaT007820.1:CDS
GATAGAAAATATTCAAACTT+TGG 0.371358 2:+23279669 None:intergenic
GCTTAATTCGACATGCGAAA+TGG 0.392046 2:-23279506 MsaT007820.1:CDS
TACTCGAATAACAGTTTCTT+CGG 0.392715 2:+23279218 None:intergenic
ATCAGATGAAGAAGCACCTT+TGG 0.404344 2:+23279647 None:intergenic
TTGGGTCAATCAATTGGGTT+GGG 0.410554 2:-23279133 MsaT007820.1:CDS
CGACATGCGAAATGGGTTAC+CGG 0.423375 2:-23279498 MsaT007820.1:CDS
GCAAACAATGGTATCCTTGT+CGG 0.427133 2:+23279527 None:intergenic
CTTAATTCGACATGCGAAAT+GGG 0.428618 2:-23279505 MsaT007820.1:CDS
AACTACTTTGTAAAATTCTA+GGG 0.432635 2:+23279601 None:intergenic
AATTAGGTGATGGGGATAAC+CGG 0.452526 2:+23279479 None:intergenic
TTTGGGTCAATCAATTGGGT+TGG 0.485642 2:-23279134 MsaT007820.1:CDS
AACTGCAGAACTGAGGCCCT+TGG 0.489139 2:+23279049 None:intergenic
TAAACTATAACAAACATTTG+TGG 0.491809 2:+23279087 None:intergenic
ACCCCAAAAGTAGTGATTGA+AGG 0.504836 2:+23279318 None:intergenic
TTTGATGTCTTTAAGAAATG+GGG 0.510130 2:-23279246 MsaT007820.1:CDS
GTTGCTTTGGCAGCCAAGAA+AGG 0.515077 2:-23279297 MsaT007820.1:CDS
ATTAGCCACAATTAGGTGAT+GGG 0.519376 2:+23279470 None:intergenic
TACATAAAAGGATCATTCCA+AGG 0.520767 2:-23279066 MsaT007820.1:CDS
TATTAGCCACAATTAGGTGA+TGG 0.529217 2:+23279469 None:intergenic
CTAGGGTTTGCAGTTAACAA+TGG 0.535070 2:+23279618 None:intergenic
TGATTTGATTAAGTCATTGG+AGG 0.558497 2:-23279364 MsaT007820.1:CDS
TTGCTAGATTGCTTCACTCA+AGG 0.565363 2:-23279407 MsaT007820.1:CDS
ACATAAAAGGATCATTCCAA+GGG 0.612722 2:-23279065 MsaT007820.1:CDS
ATGTCTTTAAGAAATGGGGT+TGG 0.618819 2:-23279242 MsaT007820.1:CDS
TTATCCCCATCACCTAATTG+TGG 0.621008 2:-23279475 MsaT007820.1:CDS
ATGTCGAATTAAGCAAACAA+TGG 0.635419 2:+23279515 None:intergenic
TTAGCCACAATTAGGTGATG+GGG 0.642610 2:+23279471 None:intergenic
CAAAAGAAACTGCAGAACTG+AGG 0.662002 2:+23279042 None:intergenic
TCAGTTGAACCAAACATACG+TGG 0.715830 2:+23279384 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 23279038 23279700 23279038 ID=MsaG007820
Chr2 mRNA 23279038 23279700 23279038 ID=MsaT007820.1;Parent=MsaG007820
Chr2 exon 23279038 23279700 23279038 ID=MsaT007820.1.exon1;Parent=MsaT007820.1
Chr2 CDS 23279038 23279700 23279038 ID=cds.MsaT007820.1;Parent=MsaT007820.1
Gene Sequence

>MsaT007820.1

ATGTTATTGCCAAAGTTTGAATATTTTCTATCCAAAGGTGCTTCTTCATCTGATATTGTTCCATTGTTAACTGCAAACCCTAGAATTTTACAAAGTAGTTTGGAGAAACGAATAATCCCTCTTTTTGAATTGTTGAATAGGTTTTCGAAAACCGACAAGGATACCATTGTTTGCTTAATTCGACATGCGAAATGGGTTACCGGTTATCCCCATCACCTAATTGTGGCTAATATCAATTTGATGACTGATTTTGGAGTTTCTGATACTAACATTGCTAGATTGCTTCACTCAAGGCCACGTATGTTTGGTTCAACTGATTTGATTAAGTCATTGGAGGAAGTCAAGTGTTTAGGGTTTGATCCTTCAATCACTACTTTTGGGGTTGCTTTGGCAGCCAAGAAAGGTATAAGTAAAAAACTTTGGGATGAGAAATTTGATGTCTTTAAGAAATGGGGTTGGTCCGAAGAAACTGTTATTCGAGTATTTAGGTATCAGCCTAATTTGATGTTGACTTCAATTGATAAGATTAATTTGGTGATGAGATTTTGGGTCAATCAATTGGGTTGGGATTCTTTGGCACTTGCTAAATGTCCACAAATGTTTGTTATAGTTTACATAAAAGGATCATTCCAAGGGCCTCAGTTCTGCAGTTTCTTTTGCTGA
Protein sequence

>MsaT007820.1

MLLPKFEYFLSKGASSSDIVPLLTANPRILQSSLEKRIIPLFELLNRFSKTDKDTIVCLIRHAKWVTGYPHHLIVANINLMTDFGVSDTNIARLLHSRPRMFGSTDLIKSLEEVKCLGFDPSITTFGVALAAKKGISKKLWDEKFDVFKKWGWSEETVIRVFRYQPNLMLTSIDKINLVMRFWVNQLGWDSLALAKCPQMFVIVYIKGSFQGPQFCSFFC*