AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007824

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007824 MtrunA17_Chr2g0296251 62.963 405 115 7 1 394 1 381 2.02e-156 454
MsaG007824 MtrunA17_Chr2g0296251 77.586 174 39 0 390 563 225 398 4.90e-88 278
MsaG007824 MtrunA17_Chr2g0296291 61.097 401 129 7 1 394 1 381 7.27e-150 438
MsaG007824 MtrunA17_Chr2g0296291 69.271 192 59 0 390 581 225 416 9.09e-84 268
MsaG007824 MtrunA17_Chr2g0296711 52.139 374 113 6 30 394 53 369 5.59e-109 333
MsaG007824 MtrunA17_Chr2g0296711 73.158 190 51 0 390 579 213 402 4.10e-93 292
MsaG007824 MtrunA17_Chr2g0296181 49.737 380 111 8 16 392 36 338 2.12e-101 313
MsaG007824 MtrunA17_Chr2g0296181 65.775 187 37 2 390 576 211 370 6.68e-68 225
MsaG007824 MtrunA17_Chr2g0296221 48.593 391 125 9 21 394 66 397 9.54e-101 312
MsaG007824 MtrunA17_Chr2g0296221 72.581 186 51 0 390 575 241 426 1.97e-87 278
MsaG007824 MtrunA17_Chr2g0296231 48.958 384 126 7 21 394 42 365 2.88e-99 307
MsaG007824 MtrunA17_Chr2g0296231 70.000 190 55 1 390 579 209 396 1.26e-84 269
MsaG007824 MtrunA17_Chr2g0296171 47.059 391 149 8 11 394 39 378 1.06e-96 301
MsaG007824 MtrunA17_Chr2g0296171 65.657 198 59 2 390 587 222 410 6.69e-78 252
MsaG007824 MtrunA17_Chr2g0296721 72.680 194 53 0 390 583 209 402 7.48e-96 298
MsaG007824 MtrunA17_Chr2g0296721 45.146 412 130 10 13 392 16 363 2.02e-93 292
MsaG007824 MtrunA17_Chr2g0315731 72.396 192 53 0 390 581 230 421 2.16e-93 293
MsaG007824 MtrunA17_Chr2g0315731 45.455 407 137 9 13 392 36 384 2.61e-92 290
MsaG007824 MtrunA17_Chr2g0296191 48.082 391 127 9 21 394 649 980 6.75e-93 307
MsaG007824 MtrunA17_Chr2g0296191 69.591 171 52 0 390 560 824 994 8.12e-72 249
MsaG007824 MtrunA17_Chr2g0296191 41.351 370 160 7 25 392 177 491 8.16e-70 244
MsaG007824 MtrunA17_Chr2g0296191 65.537 177 61 0 390 566 337 513 1.32e-69 243
MsaG007824 MtrunA17_Chr2g0296701 78.613 173 37 0 409 581 2 174 4.35e-92 281
MsaG007824 MtrunA17_Chr2g0296701 40.909 154 74 4 242 394 2 139 4.70e-22 93.6
MsaG007824 MtrunA17_Chr2g0296741 71.875 192 54 0 390 581 230 421 5.81e-92 289
MsaG007824 MtrunA17_Chr2g0296741 42.857 406 149 8 13 392 36 384 1.17e-90 286
MsaG007824 MtrunA17_Chr2g0296551 72.487 189 52 0 390 578 11 199 3.16e-91 279
MsaG007824 MtrunA17_Chr2g0296551 45.699 186 78 5 213 394 1 167 4.15e-38 139
MsaG007824 MtrunA17_Chr2g0296301 43.341 413 151 12 1 394 1 349 6.69e-90 283
MsaG007824 MtrunA17_Chr2g0296301 45.856 181 96 2 392 570 193 373 3.14e-46 167
MsaG007824 MtrunA17_Chr2g0296211 68.478 184 58 0 392 575 187 370 6.47e-83 264
MsaG007824 MtrunA17_Chr2g0296211 40.678 177 76 3 225 394 187 341 1.48e-27 114
MsaG007824 MtrunA17_Chr2g0296211 40.361 166 81 6 21 168 104 269 3.49e-22 99.0
MsaG007824 MtrunA17_Chr4g0067121 46.691 272 106 5 25 295 46 279 1.54e-70 232
MsaG007824 MtrunA17_Chr4g0067121 48.352 182 94 0 390 571 207 388 1.01e-57 198
MsaG007824 MtrunA17_Chr2g0296561 74.675 154 29 2 1 146 1 152 2.38e-70 223
MsaG007824 MtrunA17_Chr2g0296121 38.583 381 177 7 19 396 115 441 2.82e-70 234
MsaG007824 MtrunA17_Chr2g0296121 50.802 187 92 0 390 576 283 469 7.18e-57 198
MsaG007824 MtrunA17_Chr2g0296151 37.629 388 169 9 19 395 29 354 1.98e-69 229
MsaG007824 MtrunA17_Chr2g0296151 37.463 339 195 7 246 573 48 380 3.39e-62 210
MsaG007824 MtrunA17_Chr2g0295741 37.824 386 183 6 9 391 25 356 8.71e-69 228
MsaG007824 MtrunA17_Chr2g0295741 47.111 225 116 2 355 576 165 389 3.28e-62 210
MsaG007824 MtrunA17_Chr2g0296751 70.323 155 45 1 435 589 1 154 1.51e-67 217
MsaG007824 MtrunA17_Chr2g0296111 37.470 411 181 9 20 418 29 375 1.92e-67 224
MsaG007824 MtrunA17_Chr2g0296111 59.195 174 70 1 403 576 211 383 2.68e-62 210
MsaG007824 MtrunA17_Chr2g0296131 57.310 171 73 0 405 575 4 174 5.29e-61 200
MsaG007824 MtrunA17_Chr2g0296131 58.333 60 25 0 236 295 2 61 5.86e-16 76.3
MsaG007824 MtrunA17_Chr2g0295731 54.386 171 78 0 393 563 207 377 3.63e-59 202
MsaG007824 MtrunA17_Chr2g0295731 34.988 403 197 11 21 417 38 381 1.02e-57 198
MsaG007824 MtrunA17_Chr2g0295761 36.702 376 178 8 24 395 54 373 1.42e-56 195
MsaG007824 MtrunA17_Chr2g0295761 53.179 173 80 1 393 564 218 390 2.63e-51 181
MsaG007824 MtrunA17_Chr2g0296161 53.049 164 77 0 406 569 1 164 1.34e-55 185
MsaG007824 MtrunA17_Chr2g0296161 32.105 190 92 7 239 418 1 163 1.18e-15 75.1
MsaG007824 MtrunA17_Chr4g0067671 39.172 314 129 5 24 325 38 301 7.88e-53 184
MsaG007824 MtrunA17_Chr4g0067671 46.951 164 87 0 386 549 195 358 3.26e-44 161
MsaG007824 MtrunA17_Chr4g0067141 38.889 288 130 4 32 319 46 287 2.49e-52 183
MsaG007824 MtrunA17_Chr4g0067141 42.788 208 112 2 359 561 165 370 7.69e-46 166
MsaG007824 MtrunA17_Chr2g0286191 40.293 273 112 6 29 294 30 258 6.57e-47 168
MsaG007824 MtrunA17_Chr2g0286191 50.292 171 78 3 393 563 196 359 7.12e-40 149
MsaG007824 MtrunA17_Chr2g0295721 60.177 113 45 0 406 518 1 113 3.95e-41 145
MsaG007824 MtrunA17_Chr2g0295721 61.404 57 22 0 239 295 1 57 1.31e-16 76.3
MsaG007824 MtrunA17_Chr2g0286221 49.068 161 80 2 408 568 16 174 2.80e-39 142
MsaG007824 MtrunA17_Chr2g0286221 58.182 55 20 2 241 294 16 68 6.68e-11 61.6
MsaG007824 MtrunA17_Chr2g0295711 36.548 197 85 4 21 215 37 195 1.27e-30 119
MsaG007824 MtrunA17_Chr2g0295751 42.286 175 64 2 19 191 36 175 6.83e-30 123
MsaG007824 MtrunA17_Chr4g0001831 29.730 296 167 6 32 323 39 297 3.94e-29 119
MsaG007824 MtrunA17_Chr4g0001831 28.977 176 119 4 390 561 195 368 6.55e-17 82.8
MsaG007824 MtrunA17_Chr4g0067131 49.383 81 41 0 479 559 1 81 1.44e-20 86.3
MsaG007824 MtrunA17_Chr4g0067681 55.738 61 27 0 468 528 1 61 4.83e-16 72.8
MsaG007824 MtrunA17_Chr8g0377801 28.947 152 108 0 410 561 234 385 6.91e-14 73.9
MsaG007824 MtrunA17_Chr8g0377801 23.849 239 143 3 59 295 85 286 5.45e-13 71.2
MsaG007824 MtrunA17_Chr8g0377781 31.206 141 97 0 416 556 227 367 3.18e-13 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007824 AT5G07900.1 28.322 286 157 5 23 295 51 301 6.69e-30 122
MsaG007824 AT5G07900.1 37.086 151 94 1 411 560 249 399 1.91e-25 109
MsaG007824 AT1G21150.1 31.818 264 143 2 32 293 57 285 5.61e-29 119
MsaG007824 AT1G21150.1 29.437 231 144 8 342 560 163 386 3.29e-12 68.9
MsaG007824 AT1G21150.2 31.818 264 143 2 32 293 121 349 1.16e-28 119
MsaG007824 AT1G21150.2 29.437 231 144 8 342 560 227 450 2.95e-12 69.7
MsaG007824 AT1G21150.3 29.851 268 151 2 28 293 17 249 2.10e-26 111
MsaG007824 AT1G21150.3 29.437 231 144 8 342 560 127 350 3.50e-12 68.6
MsaG007824 AT1G61990.2 29.595 321 171 7 8 321 25 297 4.52e-26 111
MsaG007824 AT1G61990.2 31.313 198 97 4 393 551 202 399 4.38e-15 78.2
MsaG007824 AT1G61990.1 29.595 321 171 7 8 321 25 297 4.52e-26 111
MsaG007824 AT1G61990.1 31.313 198 97 4 393 551 202 399 4.38e-15 78.2
MsaG007824 AT3G46950.1 29.010 293 162 8 6 295 26 275 5.35e-25 108
MsaG007824 AT1G61960.1 31.698 265 143 4 32 295 53 280 1.05e-24 107
MsaG007824 AT1G61980.3 30.943 265 144 6 32 295 53 279 2.72e-24 106
MsaG007824 AT1G61980.3 28.378 222 121 4 363 546 178 399 7.21e-14 74.3
MsaG007824 AT1G61980.2 30.943 265 144 6 32 295 53 279 2.72e-24 106
MsaG007824 AT1G61980.2 28.378 222 121 4 363 546 178 399 7.21e-14 74.3
MsaG007824 AT1G61980.1 30.943 265 144 6 32 295 53 279 2.72e-24 106
MsaG007824 AT1G61980.1 28.378 222 121 4 363 546 178 399 7.21e-14 74.3
MsaG007824 AT1G61970.2 28.000 300 167 7 7 295 18 279 4.56e-24 105
MsaG007824 AT1G61970.2 28.141 199 105 3 387 548 204 401 9.21e-13 70.9
MsaG007824 AT1G61970.7 28.000 300 167 7 7 295 18 279 4.56e-24 105
MsaG007824 AT1G61970.7 28.141 199 105 3 387 548 204 401 9.21e-13 70.9
MsaG007824 AT1G61970.6 28.000 300 167 7 7 295 18 279 4.56e-24 105
MsaG007824 AT1G61970.6 28.141 199 105 3 387 548 204 401 9.21e-13 70.9
MsaG007824 AT1G61970.3 28.000 300 167 7 7 295 18 279 4.56e-24 105
MsaG007824 AT1G61970.3 28.141 199 105 3 387 548 204 401 9.21e-13 70.9
MsaG007824 AT1G61970.5 28.000 300 167 7 7 295 18 279 4.56e-24 105
MsaG007824 AT1G61970.5 28.141 199 105 3 387 548 204 401 9.21e-13 70.9
MsaG007824 AT1G61970.4 28.000 300 167 7 7 295 18 279 4.56e-24 105
MsaG007824 AT1G61970.4 28.141 199 105 3 387 548 204 401 9.21e-13 70.9
MsaG007824 AT1G61970.1 28.000 300 167 7 7 295 18 279 4.56e-24 105
MsaG007824 AT1G61970.1 28.141 199 105 3 387 548 204 401 9.21e-13 70.9
MsaG007824 AT1G62120.1 30.508 295 163 6 30 321 55 310 9.25e-23 101
MsaG007824 AT1G62110.1 30.189 265 148 5 32 295 53 281 9.29e-23 102
MsaG007824 AT1G62085.1 30.034 293 163 6 32 321 56 309 1.27e-22 101
MsaG007824 AT1G62085.2 30.034 293 163 6 32 321 56 309 1.27e-22 101
MsaG007824 AT1G62150.1 27.778 324 172 7 32 352 59 323 7.93e-22 99.4
MsaG007824 AT5G64950.1 33.136 169 107 2 390 552 198 366 4.48e-15 77.8
MsaG007824 AT5G64950.1 22.590 332 204 7 24 347 32 318 6.21e-13 71.2
MsaG007824 AT5G23930.1 26.966 267 147 7 32 295 56 277 7.76e-14 74.3
MsaG007824 AT1G62010.1 27.027 259 147 5 64 321 71 288 8.31e-13 70.9
MsaG007824 AT1G62010.1 28.272 191 100 4 393 546 193 383 3.48e-11 65.9
MsaG007824 AT1G56380.1 33.099 142 91 2 32 170 17 157 1.04e-11 67.4
MsaG007824 AT1G56380.2 33.099 142 91 2 32 170 28 168 1.23e-11 67.4

Find 104 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTTGATGACTGATTT+TGG 0.089088 2:+23318887 MsaT007824.1:CDS
GGTCCTTCATTGGCTACTTT+TGG 0.164539 2:+23320018 MsaT007824.1:CDS
TTCAATTGATAAGATTAATT+TGG 0.211307 2:+23320994 MsaT007824.1:CDS
TAGAGGAAGTTAAGTGTTTA+GGG 0.232380 2:+23318987 MsaT007824.1:CDS
TTGGAGGAAGTTAAAGGTTT+AGG 0.236931 2:+23320812 MsaT007824.1:CDS
TTGGCTATAGTTTACATAAA+AGG 0.241707 2:+23321083 MsaT007824.1:CDS
AAGCTAGTTTGTAAAATATC+AGG 0.247708 2:-23318734 None:intergenic
TTAGAGGAAGTTAAGTGTTT+AGG 0.252656 2:+23318986 MsaT007824.1:CDS
GATCCTTCAATCACTACTTT+TGG 0.257134 2:+23320839 MsaT007824.1:CDS
CTAGAAAGGCCATCTATATT+TGG 0.258714 2:+23319946 MsaT007824.1:CDS
TTTCTCTGTCGCAATGCTTT+TGG 0.260075 2:+23318272 MsaT007824.1:CDS
GAAGTTAAGGGTTTGGGGTT+TGG 0.267974 2:+23319997 MsaT007824.1:CDS
TGGAGGAAGTTAAAGGTTTA+GGG 0.271068 2:+23320813 MsaT007824.1:CDS
TTGGAGGAAGTTAAGGGTTT+GGG 0.278438 2:+23319991 MsaT007824.1:CDS
AAGTCATTGGAGGAAGTTAA+AGG 0.288064 2:+23320806 MsaT007824.1:CDS
AATTTGGTGGAATAAGTAAA+TGG 0.291352 2:-23320359 None:intergenic
TTTGGGGTTTGGTCCTTCAT+TGG 0.311462 2:+23320008 MsaT007824.1:CDS
TCCTTTGAACAAAATATAGA+TGG 0.321886 2:-23320776 None:intergenic
AAAGGATTTGATTAAGTCAT+TGG 0.323129 2:+23320793 MsaT007824.1:CDS
ATTGAAGCCAACATCAAATA+AGG 0.325234 2:-23320977 None:intergenic
AAACTTGCTCAAGAACAATT+TGG 0.328960 2:-23320375 None:intergenic
TTGGTCCAACTGGTTTGATT+AGG 0.335285 2:+23319965 MsaT007824.1:CDS
CTCAACTTCTTCAAAATTCA+CGG 0.343864 2:+23318458 MsaT007824.1:CDS
AACTGGTTTGATTAGGTCAT+TGG 0.344318 2:+23319972 MsaT007824.1:CDS
AAGCTTAATTCCAAGAACTT+AGG 0.347303 2:-23318629 None:intergenic
AAGATGTTATTCGAGCATTT+AGG 0.355683 2:+23319122 MsaT007824.1:CDS
TAGTGGTAACCCTAAGTTCT+TGG 0.369201 2:+23318619 MsaT007824.1:CDS
TGATTTGATTAAGTCATTAG+AGG 0.371105 2:+23318970 MsaT007824.1:CDS
ATATCCTCAGAATAAGTAAA+TGG 0.375368 2:-23321181 None:intergenic
CCATCTATATTTGGTCCAAC+TGG 0.377632 2:+23319955 MsaT007824.1:CDS
AAGAAGTTGAGAACTGAATC+AGG 0.382302 2:-23318446 None:intergenic
TCTTATCTCATCGATAAATT+CGG 0.385164 2:+23318362 MsaT007824.1:CDS
CAAGTATGTTTACAACATTA+AGG 0.386648 2:+23321213 MsaT007824.1:CDS
TAGGTCATTGGAGGAAGTTA+AGG 0.386684 2:+23319984 MsaT007824.1:CDS
GTTAAATGTACACTTATGTT+TGG 0.388637 2:+23321064 MsaT007824.1:CDS
AATTGATATTAGCCACAATA+AGG 0.389243 2:-23318871 None:intergenic
AAGTTGACGTCTATAAGAAA+TGG 0.393555 2:+23320912 MsaT007824.1:CDS
AGCTTAATTCCAAGAACTTA+GGG 0.393617 2:-23318628 None:intergenic
ATCAGATGAAGAAGCACCTT+TGG 0.404344 2:-23318687 None:intergenic
AGTTGACGTCTATAAGAAAT+GGG 0.412386 2:+23320913 MsaT007824.1:CDS
TCAAATGGGTTGGGATTCCT+TGG 0.413582 2:+23321036 MsaT007824.1:CDS
AGGTCATTGGAGGAAGTTAA+GGG 0.415090 2:+23319985 MsaT007824.1:CDS
GCAGAGAATGATATCCTTGT+CGG 0.415170 2:-23318807 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2:-23318582 None:intergenic
TTGGGTTAATCAAATGGGTT+GGG 0.420431 2:+23321027 MsaT007824.1:CDS
GACAGAAGATCTTACCCATT+TGG 0.421040 2:-23321317 None:intergenic
TCAGATGAACCAAATATAGA+TGG 0.421316 2:-23318950 None:intergenic
ATTGGAGGAAGTTAAGGGTT+TGG 0.439996 2:+23319990 MsaT007824.1:CDS
AATGTATGAGTAAAACATGT+TGG 0.440381 2:+23320061 MsaT007824.1:CDS
GACATTGTCCGCATGGTTAG+TGG 0.442987 2:+23318602 MsaT007824.1:CDS
TATCTTCTGATGAAAGTTTG+AGG 0.445106 2:+23320312 MsaT007824.1:CDS
ATGGCATGTCATCCACCAAA+TGG 0.451519 2:+23321302 MsaT007824.1:CDS
TTTGGGTTAATCAAATGGGT+TGG 0.454317 2:+23321026 MsaT007824.1:CDS
GTTTCAGCCTTATTTGATGT+TGG 0.461370 2:+23320970 MsaT007824.1:CDS
TTGCTAGATTGCTTCTAGAA+AGG 0.472917 2:+23319932 MsaT007824.1:intron
CCAGTTGGACCAAATATAGA+TGG 0.479635 2:-23319955 None:intergenic
TTATCTCTTTAAGCTATATG+AGG 0.482017 2:+23321246 MsaT007824.1:CDS
TGGAGGAAGTTAAGGGTTTG+GGG 0.488916 2:+23319992 MsaT007824.1:CDS
AACTGCAAAACCGAGGCCCT+TGG 0.492384 2:-23321112 None:intergenic
AAGGATCATTCCAAGGGCCT+CGG 0.500115 2:+23321102 MsaT007824.1:CDS
ATCAAAGAAGGAACAAGATA+AGG 0.500826 2:-23321345 None:intergenic
ACCCTAAAAGTAGTCGTTGA+AGG 0.504836 2:-23319016 None:intergenic
GTCCCAAAAGTAGTGATTGA+AGG 0.505609 2:-23320842 None:intergenic
AAGGATCATTCCAAGGGCAT+TGG 0.517902 2:+23320281 MsaT007824.1:CDS
TACATAAAAGGATCATTCCA+AGG 0.520767 2:+23321095 MsaT007824.1:CDS
TAAATACTTCAAATCAAAGA+AGG 0.530787 2:-23321357 None:intergenic
TGGCATGTCATCCACCAAAT+GGG 0.530912 2:+23321303 MsaT007824.1:CDS
GCCCCAAAAGTAGCCAATGA+AGG 0.534578 2:-23320021 None:intergenic
CTTGCTCAAGAACAATTTGG+TGG 0.537950 2:-23320372 None:intergenic
TGGTTTGATTAGGTCATTGG+AGG 0.539142 2:+23319975 MsaT007824.1:CDS
ATGTATGAGTAAAACATGTT+GGG 0.544119 2:+23320062 MsaT007824.1:CDS
AGAAGATCTTACCCATTTGG+TGG 0.545274 2:-23321314 None:intergenic
GTGAAGTGGTAGTAGTGGTA+AGG 0.547397 2:-23318325 None:intergenic
TATTGCACAACCAATGCCCT+TGG 0.548759 2:-23320291 None:intergenic
TGCAGAAAAGGATCATTCCA+AGG 0.555097 2:+23320274 MsaT007824.1:intron
TAGCCCACATCGCCTTATTG+TGG 0.556654 2:+23318859 MsaT007824.1:CDS
TAAACCATTTACTTATTCTG+AGG 0.560775 2:+23321177 MsaT007824.1:CDS
TACCTCTGACATTGTCCGCA+TGG 0.562085 2:+23318595 MsaT007824.1:CDS
AGCTAGTTTGTAAAATATCA+GGG 0.570802 2:-23318733 None:intergenic
GGATTTGATTAAGTCATTGG+AGG 0.571043 2:+23320796 MsaT007824.1:CDS
ATGAGATAAGACACAGCGAA+AGG 0.577670 2:-23318350 None:intergenic
TGAGATAAGACACAGCGAAA+GGG 0.594278 2:-23318349 None:intergenic
TAGCCACAATAAGGCGATGT+GGG 0.594917 2:-23318862 None:intergenic
AAAGAAGGAACAAGATAAGG+TGG 0.595199 2:-23321342 None:intergenic
TTTCTGAAGCAATTTAGCAA+TGG 0.600000 2:-23318924 None:intergenic
AATGAAATTTGCAAATACAA+AGG 0.609085 2:+23321273 MsaT007824.1:CDS
ACATAAAAGGATCATTCCAA+GGG 0.612722 2:+23321096 MsaT007824.1:CDS
TGTACATTTAACAAGTGCCA+AGG 0.619207 2:-23321053 None:intergenic
AGGGTGTGAAGTGGTAGTAG+TGG 0.620465 2:-23318330 None:intergenic
AATGACCTAATCAAACCAGT+TGG 0.631699 2:-23319970 None:intergenic
GCAAATACAAAGGAGAACAA+TGG 0.634947 2:+23321283 MsaT007824.1:CDS
GCAGAAAAGGATCATTCCAA+GGG 0.639024 2:+23320275 MsaT007824.1:intron
CACAGCGAAAGGGTGTGAAG+TGG 0.647943 2:-23318339 None:intergenic
GTCGATGTCTTTGAGAAACG+AGG 0.648037 2:+23320093 MsaT007824.1:CDS
ACGTCTATAAGAAATGGGGT+TGG 0.656465 2:+23320918 MsaT007824.1:CDS
ACATCATCAAAAGAGAACCA+TGG 0.657560 2:+23318504 MsaT007824.1:CDS
AACCATGCGGACAATGTCAG+AGG 0.657894 2:-23318597 None:intergenic
GTATCGCAGGAAAGAAGCCA+TGG 0.666550 2:-23318521 None:intergenic
CAAAAGAAACTGCAAAACCG+AGG 0.672060 2:-23321119 None:intergenic
TTAGCCACAATAAGGCGATG+TGG 0.673377 2:-23318863 None:intergenic
GTATGTTTACAACATTAAGG+AGG 0.682031 2:+23321216 MsaT007824.1:CDS
GTTGACGTCTATAAGAAATG+GGG 0.692031 2:+23320914 MsaT007824.1:CDS
GATGTTGAGAATATCTGAGT+CGG 0.696789 2:-23318486 None:intergenic
TAGGGTTACCACTAACCATG+CGG 0.739529 2:-23318610 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 23318260 23321366 23318260 ID=MsaG007824
Chr2 mRNA 23318260 23321366 23318260 ID=MsaT007824.1;Parent=MsaG007824
Chr2 exon 23318260 23319143 23318260 ID=MsaT007824.1.exon1;Parent=MsaT007824.1
Chr2 CDS 23318260 23319143 23318260 ID=cds.MsaT007824.1;Parent=MsaT007824.1
Chr2 exon 23319939 23320114 23319939 ID=MsaT007824.1.exon2;Parent=MsaT007824.1
Chr2 CDS 23319939 23320114 23319939 ID=cds.MsaT007824.1;Parent=MsaT007824.1
Chr2 exon 23320279 23320393 23320279 ID=MsaT007824.1.exon3;Parent=MsaT007824.1
Chr2 CDS 23320279 23320393 23320279 ID=cds.MsaT007824.1;Parent=MsaT007824.1
Chr2 exon 23320760 23321366 23320760 ID=MsaT007824.1.exon4;Parent=MsaT007824.1
Chr2 CDS 23320760 23321366 23320760 ID=cds.MsaT007824.1;Parent=MsaT007824.1
Gene Sequence

>MsaT007824.1

ATGCTCAAAGCATTTCTCTGTCGCAATGCTTTTGGCATATCAAATTACTCAACAACACTCAGATTCCTTACCACTACTACCACTTCACACCCTTTCGCTGTGTCTTATCTCATCGATAAATTCGGATTCTCACATGAATTCGCACTCAAAGCTTCCAAACAGCTTTATTTCAAAACCTCACAAAAACCTGATTCAGTTCTCAACTTCTTCAAAATTCACGGTTTCACCGACTCAGATATTCTCAACATCATCAAAAGAGAACCATGGCTTCTTTCCTGCGATACCCACAAAAGAATTCTCCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTACCTCTGACATTGTCCGCATGGTTAGTGGTAACCCTAAGTTCTTGGAATTAAGCTTGAAGAATCATGAAATTAAGTTTGAATTTTTTCTATCCAAAGGTGCTTCTTCATCTGATATTGTTTCATTGTTAACTGCATACCCTGATATTTTACAAACTAGCTTTGAGAAACGAATAATCCCTCTTTTTGAATTGTTAAGTAGGTTTTTGAAAACCGACAAGGATATCATTCTCTGCTTAATTCGACATTCAAAGTCGTTTATTATTAGCCCACATCGCCTTATTGTGGCTAATATCAATTTGATGACTGATTTTGGAGTTTGTGATTCTACCATTGCTAAATTGCTTCAGAAAAAGCCATCTATATTTGGTTCATCTGATTTGATTAAGTCATTAGAGGAAGTTAAGTGTTTAGGGTTTGATCCTTCAACGACTACTTTTAGGGTTGCTTTGGCAGCCAAGATATTTATGAGTAAAAAATTTTGGGATGAGAAAGTTGACGTTTTTAAGAAATGGGGTTGGTCTGATGAAGATGTTATTCGAGCATTTAGATTGCTTCTAGAAAGGCCATCTATATTTGGTCCAACTGGTTTGATTAGGTCATTGGAGGAAGTTAAGGGTTTGGGGTTTGGTCCTTCATTGGCTACTTTTGGGGCTTCTTTGATAGCCAAGAAATGTATGAGTAAAACATGTTGGGATGAGAAAGTCGATGTCTTTGAGAAACGAGAAAAGGATCATTCCAAGGGCATTGGTTGTGCAATATCTTCTGATGAAAGTTTGAGGAAAAAGATTTCAAGATTAGCTAAACCATTTACTTATTCCACCAAATTGTTCTTGAGCAAATTGCTTCAAAATAAACCATCTATATTTTGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAAGGTTTAGGGTTTGATCCTTCAATCACTACTTTTGGGACTGCTTTGATAGCTGAGAAAAGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGACGTCTATAAGAAATGGGGTTGGTCTGATAAAGATGTTATTCGAATATTTAAGTTTCAGCCTTATTTGATGTTGGCTTCAATTGATAAGATTAATTTGGTGATGAATTTTTGGGTTAATCAAATGGGTTGGGATTCCTTGGCACTTGTTAAATGTACACTTATGTTTGGCTATAGTTTACATAAAAGGATCATTCCAAGGGCCTCGGTTTTGCAGTTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCAAGCTTAGTTAAACCATTTACTTATTCTGAGGATATGTTCTTGAGCAAGTATGTTTACAACATTAAGGAGGAGTCTGATTATCTCTTTAAGCTATATGAGGAAAAAATGAAATTTGCAAATACAAAGGAGAACAATGGCATGTCATCCACCAAATGGGTAAGATCTTCTGTCATCCACCTTATCTTGTTCCTTCTTTGA
Protein sequence

>MsaT007824.1

MLKAFLCRNAFGISNYSTTLRFLTTTTTSHPFAVSYLIDKFGFSHEFALKASKQLYFKTSQKPDSVLNFFKIHGFTDSDILNIIKREPWLLSCDTHKRILPKFQFLLSKGASTSDIVRMVSGNPKFLELSLKNHEIKFEFFLSKGASSSDIVSLLTAYPDILQTSFEKRIIPLFELLSRFLKTDKDIILCLIRHSKSFIISPHRLIVANINLMTDFGVCDSTIAKLLQKKPSIFGSSDLIKSLEEVKCLGFDPSTTTFRVALAAKIFMSKKFWDEKVDVFKKWGWSDEDVIRAFRLLLERPSIFGPTGLIRSLEEVKGLGFGPSLATFGASLIAKKCMSKTCWDEKVDVFEKREKDHSKGIGCAISSDESLRKKISRLAKPFTYSTKLFLSKLLQNKPSIFCSKDLIKSLEEVKGLGFDPSITTFGTALIAEKSLSKKLWDEKVDVYKKWGWSDKDVIRIFKFQPYLMLASIDKINLVMNFWVNQMGWDSLALVKCTLMFGYSLHKRIIPRASVLQFLLMKGLRKKNASLVKPFTYSEDMFLSKYVYNIKEESDYLFKLYEEKMKFANTKENNGMSSTKWVRSSVIHLILFLL*