AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007825

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007825 MtrunA17_Chr2g0296721 86.783 401 51 2 1 400 1 400 0.0 700
MsaG007825 MtrunA17_Chr2g0315731 85.112 403 59 1 1 402 21 423 0.0 684
MsaG007825 MtrunA17_Chr2g0296741 79.156 403 83 1 1 402 21 423 0.0 643
MsaG007825 MtrunA17_Chr2g0296221 72.439 410 103 4 1 400 25 434 0.0 574
MsaG007825 MtrunA17_Chr2g0296711 70.968 403 113 2 1 399 1 403 0.0 561
MsaG007825 MtrunA17_Chr2g0296181 73.434 399 71 4 1 394 1 369 0.0 553
MsaG007825 MtrunA17_Chr2g0296191 72.680 388 98 3 1 380 608 995 0.0 552
MsaG007825 MtrunA17_Chr2g0296191 61.518 369 128 4 31 399 168 522 1.30e-143 434
MsaG007825 MtrunA17_Chr2g0296231 69.154 402 112 3 1 397 1 395 0.0 531
MsaG007825 MtrunA17_Chr2g0296171 69.948 386 109 2 4 382 10 395 0.0 520
MsaG007825 MtrunA17_Chr2g0296251 64.935 385 96 3 33 382 18 398 2.74e-166 471
MsaG007825 MtrunA17_Chr2g0296291 62.211 389 110 2 48 401 31 417 4.17e-161 459
MsaG007825 MtrunA17_Chr2g0295741 53.134 367 165 2 28 394 29 388 1.08e-129 378
MsaG007825 MtrunA17_Chr2g0296151 52.762 362 164 2 31 392 26 380 2.49e-129 377
MsaG007825 MtrunA17_Chr2g0296111 53.279 366 161 4 27 390 21 378 3.21e-125 367
MsaG007825 MtrunA17_Chr2g0296121 49.448 362 176 2 33 394 114 468 2.26e-113 339
MsaG007825 MtrunA17_Chr2g0295731 50.149 335 164 1 52 386 50 381 3.01e-111 331
MsaG007825 MtrunA17_Chr2g0296701 86.286 175 24 0 228 402 2 176 3.17e-108 315
MsaG007825 MtrunA17_Chr4g0067121 46.264 348 181 3 44 390 46 388 2.72e-106 318
MsaG007825 MtrunA17_Chr2g0295761 51.862 349 158 6 38 385 53 392 9.08e-106 317
MsaG007825 MtrunA17_Chr2g0296301 46.991 349 178 4 41 387 28 371 3.12e-105 316
MsaG007825 MtrunA17_Chr2g0296551 75.377 199 49 0 199 397 1 199 1.17e-103 305
MsaG007825 MtrunA17_Chr4g0067141 45.640 344 174 3 40 379 35 369 6.39e-95 289
MsaG007825 MtrunA17_Chr4g0067671 46.951 328 161 3 42 365 37 355 9.96e-95 288
MsaG007825 MtrunA17_Chr2g0296211 71.351 185 53 0 211 395 187 371 8.35e-90 276
MsaG007825 MtrunA17_Chr2g0296211 73.600 125 28 3 1 121 66 189 3.17e-49 171
MsaG007825 MtrunA17_Chr2g0286191 44.478 335 175 6 51 382 33 359 6.47e-79 248
MsaG007825 MtrunA17_Chr2g0296751 75.510 147 35 1 254 400 1 146 6.23e-72 223
MsaG007825 MtrunA17_Chr2g0296131 56.647 173 75 0 222 394 2 174 2.42e-63 201
MsaG007825 MtrunA17_Chr2g0296161 53.801 171 79 0 225 395 1 171 8.00e-60 192
MsaG007825 MtrunA17_Chr4g0001831 30.053 376 246 9 10 379 3 367 2.45e-44 157
MsaG007825 MtrunA17_Chr2g0286221 49.689 161 79 2 227 387 16 174 5.08e-44 151
MsaG007825 MtrunA17_Chr2g0295721 60.177 113 45 0 225 337 1 113 3.19e-42 144
MsaG007825 MtrunA17_Chr2g0295711 49.618 131 66 0 51 181 48 178 1.15e-35 130
MsaG007825 MtrunA17_Chr2g0295751 49.655 145 69 1 33 177 35 175 4.95e-34 132
MsaG007825 MtrunA17_Chr2g0296561 50.370 135 59 2 31 165 24 150 1.38e-32 120
MsaG007825 MtrunA17_Chr8g0377801 28.477 302 212 2 80 379 85 384 1.39e-31 124
MsaG007825 MtrunA17_Chr8g0377781 28.188 298 210 2 81 376 73 368 5.66e-30 119
MsaG007825 MtrunA17_Chr4g0067131 51.852 81 39 0 298 378 1 81 1.18e-21 88.6
MsaG007825 MtrunA17_Chr4g0067681 57.377 61 26 0 287 347 1 61 1.69e-17 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007825 AT5G07900.1 33.133 332 217 4 51 379 70 399 1.77e-54 186
MsaG007825 AT1G21150.1 30.130 385 253 6 5 377 4 384 4.62e-52 179
MsaG007825 AT1G21150.2 30.130 385 253 6 5 377 68 448 2.19e-51 179
MsaG007825 AT1G21150.3 31.402 328 220 3 53 377 23 348 2.56e-50 173
MsaG007825 AT1G61980.3 29.167 360 197 8 51 365 53 399 3.26e-35 134
MsaG007825 AT1G61980.2 29.167 360 197 8 51 365 53 399 3.26e-35 134
MsaG007825 AT1G61980.1 29.167 360 197 8 51 365 53 399 3.26e-35 134
MsaG007825 AT1G61990.2 29.526 359 202 7 51 370 53 399 1.93e-34 132
MsaG007825 AT1G61990.1 29.526 359 202 7 51 370 53 399 1.93e-34 132
MsaG007825 AT1G61970.2 27.809 356 207 8 51 365 53 399 2.19e-33 129
MsaG007825 AT1G61970.7 27.809 356 207 8 51 365 53 399 2.19e-33 129
MsaG007825 AT1G61970.6 27.809 356 207 8 51 365 53 399 2.19e-33 129
MsaG007825 AT1G61970.3 27.809 356 207 8 51 365 53 399 2.19e-33 129
MsaG007825 AT1G61970.5 27.809 356 207 8 51 365 53 399 2.19e-33 129
MsaG007825 AT1G61970.4 27.809 356 207 8 51 365 53 399 2.19e-33 129
MsaG007825 AT1G61970.1 27.809 356 207 8 51 365 53 399 2.19e-33 129
MsaG007825 AT5G64950.1 28.877 374 255 7 15 379 4 375 1.32e-30 121
MsaG007825 AT1G61960.1 31.349 252 154 5 51 294 53 293 8.65e-26 108
MsaG007825 AT1G62120.1 27.729 339 184 9 51 342 57 381 9.25e-26 108
MsaG007825 AT3G46950.1 28.044 271 183 4 30 297 30 291 1.15e-25 108
MsaG007825 AT1G62085.1 28.315 279 177 5 25 294 33 297 1.43e-22 99.8
MsaG007825 AT1G62085.2 28.315 279 177 5 25 294 33 297 1.43e-22 99.8
MsaG007825 AT1G62110.1 29.528 254 159 5 51 295 53 295 3.05e-22 98.6
MsaG007825 AT1G62150.1 28.016 257 157 6 51 294 59 300 1.00e-20 94.4
MsaG007825 AT5G23930.1 23.929 397 214 10 23 342 23 408 8.04e-20 91.3
MsaG007825 AT1G62010.1 25.325 308 177 7 77 342 63 359 1.65e-18 87.0
MsaG007825 AT1G56380.2 24.444 360 184 15 51 348 28 361 6.05e-11 64.3
MsaG007825 AT1G56380.1 24.444 360 184 15 51 348 17 350 6.31e-11 63.9

Find 67 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCTTATCAACAATTT+TGG 0.063826 2:+23326540 MsaT007825.1:CDS
ATCAATTTGATGTCTGATTT+TGG 0.116384 2:+23326966 MsaT007825.1:CDS
TTTCTTATAGTTTACCTAAA+AGG 0.248707 2:+23327336 MsaT007825.1:CDS
TTGGAGGAAGTTAAGTGTTT+AGG 0.257551 2:+23327065 MsaT007825.1:CDS
TGGAGGAAGTTAAGTGTTTA+GGG 0.262883 2:+23327066 MsaT007825.1:CDS
ATAACTGAATCAGGATTATC+TGG 0.265371 2:-23326621 None:intergenic
ATGCTGCATTTGAGTGAAAA+TGG 0.281235 2:-23326474 None:intergenic
AAGATGTTATTCGAGTATTT+AGG 0.329885 2:+23327201 MsaT007825.1:CDS
GCAAACAATGGTATCCTTGT+AGG 0.332542 2:-23326886 None:intergenic
AGTTGATGTCTTTAAGAAAT+GGG 0.334222 2:+23327166 MsaT007825.1:CDS
TGAGACATAAAACAAGATTC+TGG 0.344142 2:-23326404 None:intergenic
AAATGATTTGATTAAGTCAT+TGG 0.345760 2:+23327046 MsaT007825.1:CDS
TTGCTAGACTGCTTCTTAGA+AGG 0.348343 2:+23327006 MsaT007825.1:CDS
AAGTTGATGTCTTTAAGAAA+TGG 0.356329 2:+23327165 MsaT007825.1:CDS
AAAACCCAAAATTGTTGATA+AGG 0.368297 2:-23326545 None:intergenic
AATGTATGAGTAAAATACTT+TGG 0.370949 2:+23327135 MsaT007825.1:CDS
GATAGAAAATATTCAAACTT+TGG 0.371358 2:-23326744 None:intergenic
TTCAATTGATAAGATTAATC+TGG 0.385597 2:+23327247 MsaT007825.1:CDS
AAGCTACTTTGTAAAATTTG+AGG 0.399160 2:-23326813 None:intergenic
ATCAGAGGAAGAAGCACCTT+TGG 0.406640 2:-23326766 None:intergenic
TTGGGTTAATCAAATGGGTT+GGG 0.420431 2:+23327280 MsaT007825.1:CDS
TCATTTGAACAAAATATAGA+TGG 0.424258 2:-23327029 None:intergenic
AATTGCAAAACTGAGGCTCT+TGG 0.425012 2:-23327365 None:intergenic
AGATCAATACATTACACATT+TGG 0.428401 2:-23327570 None:intergenic
AGCTACTTTGTAAAATTTGA+GGG 0.434214 2:-23326812 None:intergenic
ATGTATGAGTAAAATACTTT+GGG 0.434480 2:+23327136 MsaT007825.1:CDS
AAGAAGGTGATAACTGAATC+AGG 0.441680 2:-23326630 None:intergenic
GTCCGAAAAGCGGTTGTTGA+AGG 0.442684 2:-23327095 None:intergenic
TCAAATGGGTTGGGATCCCT+TGG 0.453367 2:+23327289 MsaT007825.1:CDS
TTTGGGTTAATCAAATGGGT+TGG 0.454317 2:+23327279 MsaT007825.1:CDS
GAGACATAAAACAAGATTCT+GGG 0.458177 2:-23326403 None:intergenic
CAAATACAACGGAGAACAAT+GGG 0.473276 2:+23327537 MsaT007825.1:CDS
TTTCATCACAACCCCACAAA+AGG 0.473339 2:+23326715 MsaT007825.1:CDS
TCAATACATTACACATTTGG+TGG 0.494508 2:-23327567 None:intergenic
GTTGATGTCTTTAAGAAATG+GGG 0.513781 2:+23327167 MsaT007825.1:CDS
AAACTATAAGAAAACATAAG+TGG 0.525922 2:-23327326 None:intergenic
ATTAGCCTCTAGAACATGAT+GGG 0.526186 2:-23326943 None:intergenic
TACATTACACATTTGGTGGA+TGG 0.530490 2:-23327563 None:intergenic
CAAAGCTTTCAACAACAAGC+AGG 0.537532 2:+23326584 MsaT007825.1:CDS
TGCTGCATTTGAGTGAAAAT+GGG 0.540143 2:-23326473 None:intergenic
CCCAAAATTGTTGATAAGGT+AGG 0.546179 2:-23326541 None:intergenic
ATATTCTCAGAATAAGCAAA+TGG 0.549537 2:-23327434 None:intergenic
TATTAGCCTCTAGAACATGA+TGG 0.549790 2:-23326944 None:intergenic
ATCACCTTCTTTCAAAACCA+TGG 0.553067 2:+23326642 MsaT007825.1:CDS
ATAAGGTAGGATACAGCAAA+AGG 0.553742 2:-23326528 None:intergenic
TGATTTGATTAAGTCATTGG+AGG 0.568604 2:+23327049 MsaT007825.1:CDS
TCCGAAAAGCGGTTGTTGAA+GGG 0.570224 2:-23327094 None:intergenic
TGGAGATTTAGTAAGTTCCA+AGG 0.571492 2:-23327306 None:intergenic
ATTGAAGTCAACATCAAACT+AGG 0.571733 2:-23327230 None:intergenic
GCAAATACAACGGAGAACAA+TGG 0.572984 2:+23327536 MsaT007825.1:CDS
TTATCCCCATCATGTTCTAG+AGG 0.591171 2:+23326938 MsaT007825.1:CDS
ATTTCGAATTAAGCAAACAA+TGG 0.591852 2:-23326898 None:intergenic
TAACTGAATCAGGATTATCT+GGG 0.600797 2:-23326620 None:intergenic
ATGTCTTTAAGAAATGGGGT+TGG 0.604281 2:+23327171 MsaT007825.1:CDS
AGACATAAAACAAGATTCTG+GGG 0.614074 2:-23326402 None:intergenic
GGAGATTTAGTAAGTTCCAA+GGG 0.618354 2:-23327305 None:intergenic
TAACAATGAAACAATATCAG+AGG 0.619380 2:-23326781 None:intergenic
CAAAAGAAATTGCAAAACTG+AGG 0.625549 2:-23327372 None:intergenic
TCTAAGAAGCAGTCTAGCAA+TGG 0.630562 2:-23327003 None:intergenic
TCATCATCACAAAAGCACCT+TGG 0.652829 2:+23326688 MsaT007825.1:CDS
AATGAAACTTGCAAATACAA+CGG 0.655557 2:+23327526 MsaT007825.1:CDS
AACTGAATCAGGATTATCTG+GGG 0.669468 2:-23326619 None:intergenic
GACATAAAACAAGATTCTGG+GGG 0.686973 2:-23326401 None:intergenic
TTAGCCTCTAGAACATGATG+GGG 0.688598 2:-23326942 None:intergenic
GTGAAAATGGGAATGACAAG+TGG 0.688711 2:-23326461 None:intergenic
TATCAAAGCAGTCCGAAAAG+CGG 0.690793 2:-23327105 None:intergenic
GGTTGTGATGAAAGTAACCA+AGG 0.737190 2:-23326705 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 23326381 23327589 23326381 ID=MsaG007825
Chr2 mRNA 23326381 23327589 23326381 ID=MsaT007825.1;Parent=MsaG007825
Chr2 exon 23326381 23327589 23326381 ID=MsaT007825.1.exon1;Parent=MsaT007825.1
Chr2 CDS 23326381 23327589 23326381 ID=cds.MsaT007825.1;Parent=MsaT007825.1
Gene Sequence

>MsaT007825.1

ATGTCCAACAAATTGTTTTTCCCCCAGAATCTTGTTTTATGTCTCATCACAACAAAATCAACAACCTTAAAATTCAACAACCACTTGTCATTCCCATTTTCACTCAAATGCAGCATCACAACATCTCAAGCAGAATCAATCACACAACCTTTTGCTGTATCCTACCTTATCAACAATTTTGGGTTTTCAACTGAATCAGCTCTCAAAGCTTTCAACAACAAGCAGGTTCGCTTCAATACCCCAGATAATCCTGATTCAGTTATCACCTTCTTTCAAAACCATGGTTTTTCACACGATAACATACGCATCATCATCACAAAAGCACCTTGGTTACTTTCATCACAACCCCACAAAAGGTTGTTGCCAAAGTTTGAATATTTTCTATCCAAAGGTGCTTCTTCCTCTGATATTGTTTCATTGTTAACTTCAAACCCTCAAATTTTACAAAGTAGCTTGGAGAAACGAATAATCCCTCTTTTTGAATTGTTGAATAGGTTTTCGAAAACCTACAAGGATACCATTGTTTGCTTAATTCGAAATTCAAGATCGTTTACTGTTTATCCCCATCATGTTCTAGAGGCTAATATCAATTTGATGTCTGATTTTGGAGTTTCTGATTCTGCCATTGCTAGACTGCTTCTTAGAAGGCCATCTATATTTTGTTCAAATGATTTGATTAAGTCATTGGAGGAAGTTAAGTGTTTAGGGTTTGACCCTTCAACAACCGCTTTTCGGACTGCTTTGATAGCCAAGAAATGTATGAGTAAAATACTTTGGGATAAGAAAGTTGATGTCTTTAAGAAATGGGGTTGGTCTGATGAAGATGTTATTCGAGTATTTAGGTGTCAGCCTAGTTTGATGTTGACTTCAATTGATAAGATTAATCTGGTGATGAATTTTTGGGTTAATCAAATGGGTTGGGATCCCTTGGAACTTACTAAATCTCCACTTATGTTTTCTTATAGTTTACCTAAAAGGATCATTCCAAGAGCCTCAGTTTTGCAATTTCTTTTGATGAAAGGTTTGCGGAAAAAAAATGCGAGCTTAGGTAGACCATTTGCTTATTCTGAGAATATGTTCTTGAACAAGTGTGTTTTCAGCTTTAAGGAGGAGTCTGATTATCTGTTGAAGCTATATGACGAAAAAATGAAACTTGCAAATACAACGGAGAACAATGGGATGCCATCCACCAAATGTGTAATGTATTGA
Protein sequence

>MsaT007825.1

MSNKLFFPQNLVLCLITTKSTTLKFNNHLSFPFSLKCSITTSQAESITQPFAVSYLINNFGFSTESALKAFNNKQVRFNTPDNPDSVITFFQNHGFSHDNIRIIITKAPWLLSSQPHKRLLPKFEYFLSKGASSSDIVSLLTSNPQILQSSLEKRIIPLFELLNRFSKTYKDTIVCLIRNSRSFTVYPHHVLEANINLMSDFGVSDSAIARLLLRRPSIFCSNDLIKSLEEVKCLGFDPSTTAFRTALIAKKCMSKILWDKKVDVFKKWGWSDEDVIRVFRCQPSLMLTSIDKINLVMNFWVNQMGWDPLELTKSPLMFSYSLPKRIIPRASVLQFLLMKGLRKKNASLGRPFAYSENMFLNKCVFSFKEESDYLLKLYDEKMKLANTTENNGMPSTKCVMY*