AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007828


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007828 MtrunA17_Chr2g0296741 88.089 403 47 1 1 402 21 423 0.0 717
MsaG007828 MtrunA17_Chr2g0315731 84.119 403 63 1 1 402 21 423 0.0 665
MsaG007828 MtrunA17_Chr2g0296721 82.793 401 67 2 1 400 1 400 0.0 655
MsaG007828 MtrunA17_Chr2g0296221 72.927 410 101 4 1 400 25 434 0.0 580
MsaG007828 MtrunA17_Chr2g0296191 73.385 387 95 3 1 379 608 994 0.0 561
MsaG007828 MtrunA17_Chr2g0296191 62.393 351 123 3 31 381 168 509 3.85e-140 425
MsaG007828 MtrunA17_Chr2g0296711 68.983 403 121 2 1 399 1 403 0.0 542
MsaG007828 MtrunA17_Chr2g0296171 70.984 386 105 2 4 382 10 395 0.0 530
MsaG007828 MtrunA17_Chr2g0296231 66.169 402 124 3 1 397 1 395 3.35e-179 504
MsaG007828 MtrunA17_Chr2g0296181 67.669 399 94 4 1 394 1 369 1.68e-177 499
MsaG007828 MtrunA17_Chr2g0296291 62.468 389 109 2 48 401 31 417 8.20e-160 456
MsaG007828 MtrunA17_Chr2g0296251 60.859 396 113 4 25 382 7 398 1.74e-156 447
MsaG007828 MtrunA17_Chr2g0296151 51.240 363 170 2 32 394 27 382 4.90e-125 366
MsaG007828 MtrunA17_Chr2g0296111 51.913 366 166 4 27 390 21 378 1.72e-121 357
MsaG007828 MtrunA17_Chr2g0295741 51.515 363 169 2 28 390 29 384 2.47e-121 357
MsaG007828 MtrunA17_Chr2g0296121 49.727 366 173 2 29 394 114 468 6.81e-117 348
MsaG007828 MtrunA17_Chr2g0295731 50.445 337 160 2 52 386 50 381 1.04e-112 335
MsaG007828 MtrunA17_Chr4g0067121 46.839 348 179 3 44 390 46 388 2.51e-108 324
MsaG007828 MtrunA17_Chr2g0296301 47.278 349 177 4 41 387 28 371 2.48e-107 321
MsaG007828 MtrunA17_Chr2g0295761 49.856 347 164 6 38 383 53 390 2.48e-100 303
MsaG007828 MtrunA17_Chr2g0296701 82.286 175 31 0 228 402 2 176 8.22e-100 294
MsaG007828 MtrunA17_Chr2g0296551 72.362 199 55 0 199 397 1 199 4.20e-97 288
MsaG007828 MtrunA17_Chr4g0067671 45.679 324 171 2 42 365 37 355 1.76e-92 282
MsaG007828 MtrunA17_Chr4g0067141 45.000 340 182 2 40 379 35 369 2.94e-92 282
MsaG007828 MtrunA17_Chr2g0296211 63.415 205 72 1 194 395 167 371 3.99e-83 258
MsaG007828 MtrunA17_Chr2g0286191 43.402 341 170 8 51 382 33 359 4.59e-77 243
MsaG007828 MtrunA17_Chr2g0296751 70.748 147 42 1 254 400 1 146 1.23e-65 206
MsaG007828 MtrunA17_Chr2g0296131 53.614 166 77 0 229 394 9 174 9.54e-58 186
MsaG007828 MtrunA17_Chr2g0296161 50.292 171 85 0 225 395 1 171 4.66e-55 179
MsaG007828 MtrunA17_Chr4g0001831 30.790 367 241 8 17 379 10 367 2.71e-45 160
MsaG007828 MtrunA17_Chr2g0286221 47.205 161 83 2 227 387 16 174 2.03e-41 144
MsaG007828 MtrunA17_Chr2g0295721 55.752 113 50 0 225 337 1 113 1.03e-40 140
MsaG007828 MtrunA17_Chr8g0377801 29.139 302 210 2 80 379 85 384 4.86e-36 136
MsaG007828 MtrunA17_Chr2g0295711 44.371 151 81 2 51 201 48 195 9.27e-36 130
MsaG007828 MtrunA17_Chr8g0377781 29.195 298 207 2 81 376 73 368 8.93e-34 129
MsaG007828 MtrunA17_Chr2g0296561 51.111 135 58 2 31 165 24 150 5.39e-33 121
MsaG007828 MtrunA17_Chr2g0295751 48.276 145 71 1 33 177 35 175 7.33e-31 123
MsaG007828 MtrunA17_Chr4g0067131 50.617 81 40 0 298 378 1 81 1.24e-22 91.3
MsaG007828 MtrunA17_Chr4g0067681 55.738 61 27 0 287 347 1 61 4.70e-17 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007828 AT5G07900.1 33.735 332 215 4 51 379 70 399 1.65e-57 194
MsaG007828 AT1G21150.1 30.857 350 231 4 37 377 37 384 6.60e-51 176
MsaG007828 AT1G21150.2 30.857 350 231 4 37 377 101 448 3.70e-50 175
MsaG007828 AT1G21150.3 31.707 328 219 3 53 377 23 348 4.40e-49 170
MsaG007828 AT5G64950.1 29.412 374 243 8 20 379 9 375 9.51e-38 140
MsaG007828 AT1G61980.3 28.814 354 206 7 51 365 53 399 3.23e-35 134
MsaG007828 AT1G61980.2 28.814 354 206 7 51 365 53 399 3.23e-35 134
MsaG007828 AT1G61980.1 28.814 354 206 7 51 365 53 399 3.23e-35 134
MsaG007828 AT1G61990.2 28.726 369 210 8 51 379 53 408 1.88e-32 127
MsaG007828 AT1G61990.1 28.726 369 210 8 51 379 53 408 1.88e-32 127
MsaG007828 AT1G61970.2 27.042 355 211 9 51 365 53 399 8.01e-32 125
MsaG007828 AT1G61970.7 27.042 355 211 9 51 365 53 399 8.01e-32 125
MsaG007828 AT1G61970.6 27.042 355 211 9 51 365 53 399 8.01e-32 125
MsaG007828 AT1G61970.3 27.042 355 211 9 51 365 53 399 8.01e-32 125
MsaG007828 AT1G61970.5 27.042 355 211 9 51 365 53 399 8.01e-32 125
MsaG007828 AT1G61970.4 27.042 355 211 9 51 365 53 399 8.01e-32 125
MsaG007828 AT1G61970.1 27.042 355 211 9 51 365 53 399 8.01e-32 125
MsaG007828 AT1G62120.1 26.524 328 202 6 51 342 57 381 2.70e-25 107
MsaG007828 AT3G46950.1 28.782 271 181 4 30 297 30 291 4.87e-25 106
MsaG007828 AT1G61960.1 29.388 245 168 3 51 294 53 293 6.05e-22 97.8
MsaG007828 AT1G62085.1 28.044 271 188 4 25 294 33 297 1.43e-20 93.6
MsaG007828 AT1G62085.2 28.044 271 188 4 25 294 33 297 1.43e-20 93.6
MsaG007828 AT1G62150.1 27.347 245 174 4 51 294 59 300 1.57e-20 93.6
MsaG007828 AT5G23930.1 26.374 273 183 6 19 284 19 280 5.13e-19 89.0
MsaG007828 AT1G62010.1 25.157 318 194 6 62 342 49 359 5.16e-19 88.6
MsaG007828 AT1G62110.1 27.200 250 170 4 51 295 53 295 9.22e-19 88.2

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTTGATGGTTGATTT+TGG 0.116384 2:+23341573 MsaT007828.1:CDS
TCCTACCTTATCAACAATTT+TGG 0.146770 2:+23341147 MsaT007828.1:CDS
CGAACAAGGCCATCTATATT+TGG 0.170334 2:+23341627 MsaT007828.1:CDS
AAACTACTTTGTAAAATTCT+AGG 0.206540 2:-23341420 None:intergenic
GACCCTTCAACGACCGCTTT+TGG 0.242026 2:+23341699 MsaT007828.1:CDS
ATGACTGAGTCAGGCTTATC+TGG 0.244478 2:-23341228 None:intergenic
ATGCTGCATTTGAGTGAAAA+TGG 0.281235 2:-23341081 None:intergenic
TTGTTTGCTTAATTCGATAT+TGG 0.297090 2:+23341508 MsaT007828.1:CDS
AAGATGTTATTCGAGTATTT+AGG 0.310453 2:+23341808 MsaT007828.1:CDS
AACTGATTTGATTAAGTCAT+TGG 0.334181 2:+23341653 MsaT007828.1:CDS
GCAAACAATGATATCCTTGT+TGG 0.346453 2:-23341493 None:intergenic
TTGGAGGAAGTTAAGGGCTT+AGG 0.351722 2:+23341672 MsaT007828.1:CDS
ATTAGACACAATAAGGTGAT+AGG 0.358420 2:-23341550 None:intergenic
TGAGACATAAAACAGGATTC+TGG 0.372524 2:-23341011 None:intergenic
AATTGATATTAGACACAATA+AGG 0.377379 2:-23341557 None:intergenic
TGTGTCTAATATCAATTTGA+TGG 0.381516 2:+23341563 MsaT007828.1:CDS
TAAGTCATTGGAGGAAGTTA+AGG 0.400812 2:+23341665 MsaT007828.1:CDS
AAGTTGACGTCTTTATCAAA+TGG 0.402844 2:+23341772 MsaT007828.1:CDS
AATGGATGAGTAACATACTT+TGG 0.408464 2:+23341742 MsaT007828.1:CDS
TTGGGTTAATCAAATGGGTT+GGG 0.412996 2:+23341887 MsaT007828.1:CDS
TGGAGGAAGTTAAGGGCTTA+GGG 0.415402 2:+23341673 MsaT007828.1:CDS
AAGAAGTTGATGACTGAGTC+AGG 0.419249 2:-23341237 None:intergenic
AGTTGACGTCTTTATCAAAT+GGG 0.424416 2:+23341773 MsaT007828.1:CDS
AATTGCAAAACTGAGGCTCT+TGG 0.425012 2:-23341972 None:intergenic
GTCCCAAAAGCGGTCGTTGA+AGG 0.427635 2:-23341702 None:intergenic
AGATCAATACATTACACATT+TGG 0.428401 2:-23342177 None:intergenic
TCAGTTGAACCAAATATAGA+TGG 0.441181 2:-23341636 None:intergenic
AAGTCATTGGAGGAAGTTAA+GGG 0.446904 2:+23341666 MsaT007828.1:CDS
ATGGATGAGTAACATACTTT+GGG 0.450437 2:+23341743 MsaT007828.1:CDS
AGAAACCAAAATTGTTGATA+AGG 0.456851 2:-23341152 None:intergenic
AACTACTTTGTAAAATTCTA+GGG 0.461652 2:-23341419 None:intergenic
GAGACATAAAACAGGATTCT+GGG 0.469385 2:-23341010 None:intergenic
TTTGGGTTAATCAAATGGGT+TGG 0.473047 2:+23341886 MsaT007828.1:CDS
TCAAATGGGTTGGGATCCCT+TGG 0.473886 2:+23341896 MsaT007828.1:CDS
TATGTTACTCATCCATTGCT+TGG 0.480966 2:-23341736 None:intergenic
GTTGTGATGAGACATAAAAC+AGG 0.492504 2:-23341018 None:intergenic
TCAATACATTACACATTTGG+TGG 0.494508 2:-23342174 None:intergenic
AAACTAAAAGAAAACATAAG+TGG 0.504288 2:-23341933 None:intergenic
TGACTGAGTCAGGCTTATCT+GGG 0.518425 2:-23341227 None:intergenic
GAAAGGTTTGCGAAAGAAGA+AGG 0.527252 2:+23342004 MsaT007828.1:CDS
ATATCCTCAGAATAAGCAAA+TGG 0.530372 2:-23342041 None:intergenic
TACATTACACATTTGGTGGA+TGG 0.530490 2:-23342170 None:intergenic
TGCTGCATTTGAGTGAAAAT+GGG 0.540143 2:-23341080 None:intergenic
GAAAGCTTTCAACCTCAAGC+AGG 0.544441 2:+23341191 MsaT007828.1:CDS
TTTGATGATGATGCGTGTGT+TGG 0.546473 2:-23341283 None:intergenic
ATAAGGTAGGATACAGCAAA+TGG 0.555873 2:-23341135 None:intergenic
ACCAAAATTGTTGATAAGGT+AGG 0.556095 2:-23341148 None:intergenic
TGATTTGATTAAGTCATTGG+AGG 0.558497 2:+23341656 MsaT007828.1:CDS
TTGCTAGATTGCTTCGAACA+AGG 0.560619 2:+23341613 MsaT007828.1:CDS
TGGAGAATTAATAAGTGCCA+AGG 0.561400 2:-23341913 None:intergenic
TTTCAGCTTTAAGGAGGAGT+CGG 0.563069 2:+23342082 MsaT007828.1:CDS
ACGTCTTTATCAAATGGGGT+TGG 0.563703 2:+23341778 MsaT007828.1:CDS
TCCCAAAAGCGGTCGTTGAA+GGG 0.570224 2:-23341701 None:intergenic
TTAGACACAATAAGGTGATA+GGG 0.570881 2:-23341549 None:intergenic
TAGACCATTTGCTTATTCTG+AGG 0.571730 2:+23342037 MsaT007828.1:CDS
ATTGACGTCAACATCAAACT+AGG 0.584788 2:-23341837 None:intergenic
TGTTGGAGTTTGAAAAGCCA+TGG 0.592773 2:-23341266 None:intergenic
CTGCTTTGGTGACCAAGCAA+TGG 0.599773 2:+23341724 MsaT007828.1:CDS
TATTGAAACGAACCTGCTTG+AGG 0.609752 2:-23341203 None:intergenic
GTTGACGTCTTTATCAAATG+GGG 0.617322 2:+23341774 MsaT007828.1:CDS
CTAGGGTGTGCAGTTAACAA+TGG 0.619109 2:-23341402 None:intergenic
TAACAATGGAATAATATCAG+AGG 0.624344 2:-23341388 None:intergenic
CAAAAGAAATTGCAAAACTG+AGG 0.625549 2:-23341979 None:intergenic
GGAGAATTAATAAGTGCCAA+GGG 0.628472 2:-23341912 None:intergenic
GTGAAAATGGGAATGAGAAG+TGG 0.636779 2:-23341068 None:intergenic
TGTTCGAAGCAATCTAGCAA+TGG 0.646698 2:-23341610 None:intergenic
AATGAAACTTGCAAATACAA+CGG 0.655557 2:+23342133 MsaT007828.1:CDS
CACCAAAGCAGTCCCAAAAG+CGG 0.659565 2:-23341712 None:intergenic
GACTGAGTCAGGCTTATCTG+GGG 0.674143 2:-23341226 None:intergenic
TAGGTTCTTGATAACCAACA+AGG 0.696135 2:+23341479 MsaT007828.1:CDS
GGTTGTGATGAAAGTAACCA+AGG 0.737190 2:-23341312 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 23340988 23342196 23340988 ID=MsaG007828
Chr2 mRNA 23340988 23342196 23340988 ID=MsaT007828.1;Parent=MsaG007828
Chr2 exon 23340988 23342196 23340988 ID=MsaT007828.1.exon1;Parent=MsaT007828.1
Chr2 CDS 23340988 23342196 23340988 ID=cds.MsaT007828.1;Parent=MsaT007828.1
Gene Sequence

>MsaT007828.1

ATGTCCAACAAATTGTTTTTCTCCCAGAATCCTGTTTTATGTCTCATCACAACAAAATCAACAACCTTAAAATTCAAAAACCACTTCTCATTCCCATTTTCACTCAAATGCAGCATCACAACATCTCAAGCAGAATCAATCACACAACCATTTGCTGTATCCTACCTTATCAACAATTTTGGTTTCTCGTCTGAATCAGCTCTGAAAGCTTTCAACCTCAAGCAGGTTCGTTTCAATACCCCAGATAAGCCTGACTCAGTCATCAACTTCTTAAAAAACCATGGCTTTTCAAACTCCAACACACGCATCATCATCAAAAAAGCACCTTGGTTACTTTCATCACAACCCCACAAAATGTTATTGCCAAAGTTTCAATTTTTTCTATCTGATGGCGCTTATTCCTCTGATATTATTCCATTGTTAACTGCACACCCTAGAATTTTACAAAGTAGTTTGGAGAAACGAATAATCCCTCTTTTTGAATTATTAAGTAGGTTCTTGATAACCAACAAGGATATCATTGTTTGCTTAATTCGATATTGGACTGCATTTTCTACTAATCCCTATCACCTTATTGTGTCTAATATCAATTTGATGGTTGATTTTGGAGTTTCTGATTCTACCATTGCTAGATTGCTTCGAACAAGGCCATCTATATTTGGTTCAACTGATTTGATTAAGTCATTGGAGGAAGTTAAGGGCTTAGGGTTTGACCCTTCAACGACCGCTTTTGGGACTGCTTTGGTGACCAAGCAATGGATGAGTAACATACTTTGGGATAAGAAAGTTGACGTCTTTATCAAATGGGGTTGGTCTGATGAAGATGTTATTCGAGTATTTAGGAGTCAGCCTAGTTTGATGTTGACGTCAATTGATAAAATTAATCTGTTGATGAGTTTTTGGGTTAATCAAATGGGTTGGGATCCCTTGGCACTTATTAATTCTCCACTTATGTTTTCTTTTAGTTTATCTAAAAGGATCATTCCAAGAGCCTCAGTTTTGCAATTTCTTTTGATGAAAGGTTTGCGAAAGAAGAAGGCGAGCTTAGTTAGACCATTTGCTTATTCTGAGGATATGTTCTTGAACAAGTGTGTTTTCAGCTTTAAGGAGGAGTCGGATTCTCTGTTGAAGCTATATGACGAAAAAATGAAACTTGCAAATACAACGGAGAACAATGTGATGCCATCCACCAAATGTGTAATGTATTGA

Protein sequence

>MsaT007828.1

MSNKLFFSQNPVLCLITTKSTTLKFKNHFSFPFSLKCSITTSQAESITQPFAVSYLINNFGFSSESALKAFNLKQVRFNTPDKPDSVINFLKNHGFSNSNTRIIIKKAPWLLSSQPHKMLLPKFQFFLSDGAYSSDIIPLLTAHPRILQSSLEKRIIPLFELLSRFLITNKDIIVCLIRYWTAFSTNPYHLIVSNINLMVDFGVSDSTIARLLRTRPSIFGSTDLIKSLEEVKGLGFDPSTTAFGTALVTKQWMSNILWDKKVDVFIKWGWSDEDVIRVFRSQPSLMLTSIDKINLLMSFWVNQMGWDPLALINSPLMFSFSLSKRIIPRASVLQFLLMKGLRKKKASLVRPFAYSEDMFLNKCVFSFKEESDSLLKLYDEKMKLANTTENNVMPSTKCVMY*