AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007829

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007829 MtrunA17_Chr2g0296291 72.967 418 107 3 1 414 1 416 0.0 605
MsaG007829 MtrunA17_Chr2g0296251 75.250 400 93 2 1 396 1 398 0.0 587
MsaG007829 MtrunA17_Chr2g0296711 66.580 386 94 1 28 413 53 403 1.57e-180 509
MsaG007829 MtrunA17_Chr2g0296221 63.868 393 103 3 26 414 77 434 1.22e-173 492
MsaG007829 MtrunA17_Chr2g0315731 64.359 390 104 1 27 416 69 423 5.04e-172 488
MsaG007829 MtrunA17_Chr2g0296721 64.691 388 101 2 27 414 49 400 5.67e-171 484
MsaG007829 MtrunA17_Chr2g0296741 61.856 388 113 1 27 414 69 421 5.91e-169 480
MsaG007829 MtrunA17_Chr2g0296231 60.636 409 116 4 6 411 29 395 8.87e-165 468
MsaG007829 MtrunA17_Chr2g0296171 62.634 372 102 2 27 396 59 395 3.02e-157 449
MsaG007829 MtrunA17_Chr2g0296191 62.973 370 100 2 26 393 660 994 4.57e-153 459
MsaG007829 MtrunA17_Chr2g0296191 54.453 393 132 5 22 413 176 522 4.21e-126 389
MsaG007829 MtrunA17_Chr2g0296181 60.471 382 89 3 27 408 50 369 2.12e-146 421
MsaG007829 MtrunA17_Chr2g0296301 48.175 411 163 7 1 401 1 371 7.01e-125 367
MsaG007829 MtrunA17_Chr2g0296151 47.368 399 166 3 14 406 20 380 4.27e-122 359
MsaG007829 MtrunA17_Chr2g0295741 48.312 385 161 2 24 408 42 388 1.66e-119 353
MsaG007829 MtrunA17_Chr2g0296111 49.608 383 152 4 24 404 35 378 4.08e-119 352
MsaG007829 MtrunA17_Chr2g0296121 48.052 385 162 2 24 408 122 468 8.21e-118 351
MsaG007829 MtrunA17_Chr2g0295731 46.154 377 165 3 24 400 43 381 1.05e-112 335
MsaG007829 MtrunA17_Chr4g0067121 44.503 382 171 4 24 404 47 388 5.88e-107 321
MsaG007829 MtrunA17_Chr2g0296551 77.387 199 45 0 213 411 1 199 4.34e-106 311
MsaG007829 MtrunA17_Chr2g0296701 80.571 175 34 0 242 416 2 176 9.50e-100 294
MsaG007829 MtrunA17_Chr2g0295761 46.070 369 158 5 30 397 62 390 3.13e-96 293
MsaG007829 MtrunA17_Chr2g0296211 68.075 213 65 1 200 409 159 371 5.17e-96 292
MsaG007829 MtrunA17_Chr2g0296211 31.220 205 85 3 21 172 104 305 3.13e-22 97.4
MsaG007829 MtrunA17_Chr4g0067141 40.659 364 176 3 30 393 46 369 2.91e-84 262
MsaG007829 MtrunA17_Chr4g0067671 41.477 352 166 3 28 379 44 355 1.78e-82 257
MsaG007829 MtrunA17_Chr2g0286191 41.129 372 175 7 27 396 30 359 3.07e-77 244
MsaG007829 MtrunA17_Chr2g0296751 74.830 147 36 1 268 414 1 146 2.62e-73 227
MsaG007829 MtrunA17_Chr2g0296561 70.130 154 36 2 1 146 1 152 2.35e-68 213
MsaG007829 MtrunA17_Chr2g0296161 54.386 171 78 0 239 409 1 171 2.98e-61 196
MsaG007829 MtrunA17_Chr2g0296131 55.422 166 74 0 243 408 9 174 4.56e-60 193
MsaG007829 MtrunA17_Chr2g0295721 57.522 113 48 0 239 351 1 113 1.85e-40 140
MsaG007829 MtrunA17_Chr2g0286221 46.914 162 82 3 241 401 16 174 4.43e-39 138
MsaG007829 MtrunA17_Chr4g0001831 27.717 368 223 6 30 393 39 367 1.06e-36 137
MsaG007829 MtrunA17_Chr2g0295711 36.279 215 90 4 10 215 19 195 5.85e-33 123
MsaG007829 MtrunA17_Chr2g0295751 40.571 175 67 2 19 191 36 175 7.56e-29 117
MsaG007829 MtrunA17_Chr8g0377801 25.519 337 212 3 59 393 85 384 2.66e-27 112
MsaG007829 MtrunA17_Chr8g0377781 27.410 332 202 4 61 390 74 368 9.17e-26 107
MsaG007829 MtrunA17_Chr4g0067131 49.383 81 41 0 312 392 1 81 1.72e-20 85.5
MsaG007829 MtrunA17_Chr4g0067681 50.820 61 30 0 301 361 1 61 6.25e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007829 AT5G07900.1 29.973 367 217 4 30 393 70 399 4.94e-50 174
MsaG007829 AT1G21150.1 30.790 367 210 5 30 391 57 384 7.65e-43 155
MsaG007829 AT1G21150.3 30.541 370 213 5 27 391 18 348 1.48e-42 153
MsaG007829 AT1G21150.2 30.790 367 210 5 30 391 121 448 1.98e-42 155
MsaG007829 AT1G61990.2 26.389 432 226 8 7 393 24 408 1.60e-31 124
MsaG007829 AT1G61990.1 26.389 432 226 8 7 393 24 408 1.60e-31 124
MsaG007829 AT1G61980.3 26.992 389 203 7 30 379 53 399 3.90e-29 118
MsaG007829 AT1G61980.2 26.992 389 203 7 30 379 53 399 3.90e-29 118
MsaG007829 AT1G61980.1 26.992 389 203 7 30 379 53 399 3.90e-29 118
MsaG007829 AT1G61970.2 25.349 430 216 11 7 379 18 399 3.19e-27 112
MsaG007829 AT1G61970.7 25.349 430 216 11 7 379 18 399 3.19e-27 112
MsaG007829 AT1G61970.6 25.349 430 216 11 7 379 18 399 3.19e-27 112
MsaG007829 AT1G61970.3 25.349 430 216 11 7 379 18 399 3.19e-27 112
MsaG007829 AT1G61970.5 25.349 430 216 11 7 379 18 399 3.19e-27 112
MsaG007829 AT1G61970.4 25.349 430 216 11 7 379 18 399 3.19e-27 112
MsaG007829 AT1G61970.1 25.349 430 216 11 7 379 18 399 3.19e-27 112
MsaG007829 AT5G64950.1 26.705 352 240 7 57 393 27 375 4.05e-26 109
MsaG007829 AT1G61960.1 25.412 425 272 12 9 393 32 451 5.40e-23 100
MsaG007829 AT1G62120.1 25.613 367 197 8 27 356 54 381 3.59e-19 89.7
MsaG007829 AT1G62085.1 28.125 288 152 5 30 308 56 297 1.04e-18 88.2
MsaG007829 AT1G62085.2 28.125 288 152 5 30 308 56 297 1.04e-18 88.2
MsaG007829 AT5G23930.1 24.935 385 245 10 30 379 56 431 4.91e-18 86.3
MsaG007829 AT1G62110.1 26.837 313 182 7 5 309 22 295 2.13e-17 84.3
MsaG007829 AT1G62150.1 25.694 288 159 5 30 308 59 300 4.08e-17 83.6
MsaG007829 AT1G62010.1 24.576 354 188 8 64 379 71 383 4.14e-17 83.2
MsaG007829 AT3G46950.1 24.759 311 184 8 6 311 26 291 3.12e-16 80.9
MsaG007829 AT1G56380.1 25.199 377 230 17 30 379 17 368 2.43e-13 71.6
MsaG007829 AT1G56380.2 25.199 377 230 17 30 379 28 379 2.80e-13 71.6
MsaG007829 AT2G44020.1 23.779 307 207 8 66 357 147 441 1.36e-12 69.7

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACTAGGCCATCTATATT+TGG 0.156515 2:+23344932 MsaT007829.1:CDS
GATCCTTCATTGGCCACTTT+TGG 0.161610 2:+23345004 MsaT007829.1:CDS
CTGAGCGTTGTTGAAGTATT+TGG 0.204791 2:-23344290 None:intergenic
TAGAGGAAGTTAAGGGTTTA+GGG 0.232380 2:+23344978 MsaT007829.1:CDS
TTTCTTATAGTTTACATAAA+AGG 0.235192 2:+23345248 MsaT007829.1:CDS
TTAGAGGAAGTTAAGGGTTT+AGG 0.237816 2:+23344977 MsaT007829.1:CDS
GCAAACAATGGTATCCTTAC+TGG 0.255560 2:-23344798 None:intergenic
TTTCTCTGTCGCAATGCTTT+TGG 0.260075 2:+23344263 MsaT007829.1:CDS
AAGAATTTGACAACTGAATC+TGG 0.293509 2:-23344437 None:intergenic
AAGCTGTTATTCGAGTATTT+AGG 0.318007 2:+23345113 MsaT007829.1:CDS
AAGCTAATGTGTAAAATTGT+AGG 0.327898 2:-23344725 None:intergenic
TGAATCTGACATTGTTCATA+TGG 0.329984 2:+23344586 MsaT007829.1:CDS
GACATTGTTCATATGGTTAG+AGG 0.340870 2:+23344593 MsaT007829.1:CDS
TAGAGGTAACCCTAAGTTCT+TGG 0.343348 2:+23344610 MsaT007829.1:CDS
AAGCTTAATTCCAAGAACTT+AGG 0.347303 2:-23344620 None:intergenic
TCCTACCTCGTCGACAAATT+CGG 0.354152 2:+23344347 MsaT007829.1:CDS
GTCAGATTCAGAAGCACCTT+TGG 0.364937 2:-23344573 None:intergenic
AACTTCTCAGAATAAGTAAA+TGG 0.371916 2:-23345346 None:intergenic
AGATTTGATTAAGTCATTAG+AGG 0.374392 2:+23344961 MsaT007829.1:CDS
AGATCAAAATCTTATACATT+TGG 0.374741 2:-23345482 None:intergenic
TAAGTCATTAGAGGAAGTTA+AGG 0.381295 2:+23344970 MsaT007829.1:CDS
GGTTGACGACTTTAAGAAAT+GGG 0.386387 2:+23345078 MsaT007829.1:CDS
AGCTTAATTCCAAGAACTTA+GGG 0.393617 2:-23344619 None:intergenic
ATCAGATGAAGAAGCACCTT+TGG 0.404344 2:-23344678 None:intergenic
TTGGGTTAATCAAATGGGTT+GGG 0.412996 2:+23345192 MsaT007829.1:CDS
TCAAATGGGTTGGGATTCCT+TGG 0.413582 2:+23345201 MsaT007829.1:CDS
TTTAGGGTTTGATCCTTCAT+TGG 0.422453 2:+23344994 MsaT007829.1:CDS
AAGTCATTAGAGGAAGTTAA+GGG 0.427528 2:+23344971 MsaT007829.1:CDS
AGGTTGACGACTTTAAGAAA+TGG 0.446927 2:+23345077 MsaT007829.1:CDS
TCAAAATCTTATACATTTGG+TGG 0.453156 2:-23345479 None:intergenic
TTGCTAGATTGCTTCAAACT+AGG 0.457873 2:+23344918 MsaT007829.1:CDS
CAAAAGAAATTGCAAAACTA+AGG 0.463398 2:-23345284 None:intergenic
TTTGGGTTAATCAAATGGGT+TGG 0.473047 2:+23345191 MsaT007829.1:CDS
CAAATACAACGGAGAACAAT+GGG 0.473276 2:+23345449 MsaT007829.1:CDS
ACCCCAAAAGTGGCCAATGA+AGG 0.496707 2:-23345007 None:intergenic
AATTGCAAAACTAAGGCCCT+TGG 0.498501 2:-23345277 None:intergenic
ATGGAAACCAACAAAATACT+AGG 0.512435 2:-23345142 None:intergenic
AAACTATAAGAAAACATAAG+TGG 0.515137 2:-23345238 None:intergenic
AATTGATATTAGCCACCATA+AGG 0.515847 2:-23344862 None:intergenic
TACATAAAAGGATCATTCCA+AGG 0.520767 2:+23345260 MsaT007829.1:CDS
AATCTTATACATTTGGTGGA+TGG 0.528179 2:-23345475 None:intergenic
TAGGTTCTTCAAATCCAGTA+AGG 0.543751 2:+23344784 MsaT007829.1:CDS
AAGGGTGTGAAGCGATGGTA+AGG 0.546793 2:-23344316 None:intergenic
AGCGAAAGGGTGTGAAGCGA+TGG 0.554065 2:-23344321 None:intergenic
TACCAAAGCAACCCCAAAAG+TGG 0.559263 2:-23345017 None:intergenic
TTTCAGCTTTAAGGAGGAGT+CGG 0.563069 2:+23345387 MsaT007829.1:CDS
GCAAATACAACGGAGAACAA+TGG 0.572984 2:+23345448 MsaT007829.1:CDS
TCCGAATTTGTCGACGAGGT+AGG 0.584234 2:-23344348 None:intergenic
CGAGGTAGGACACAGCGAAA+GGG 0.593390 2:-23344334 None:intergenic
ACGACTTTAAGAAATGGGGT+TGG 0.600597 2:+23345083 MsaT007829.1:CDS
TAATTGCCATCGCCTTATGG+TGG 0.603597 2:+23344850 MsaT007829.1:CDS
ACGAGGTAGGACACAGCGAA+AGG 0.604629 2:-23344335 None:intergenic
ACATAAAAGGATCATTCCAA+GGG 0.612722 2:+23345261 MsaT007829.1:CDS
GTTGACGACTTTAAGAAATG+GGG 0.629997 2:+23345079 MsaT007829.1:CDS
TTCACTGACTCAGACATGAA+AGG 0.637892 2:+23344473 MsaT007829.1:CDS
TGGACATTTAGTAAGTGCCA+AGG 0.640725 2:-23345218 None:intergenic
AGTTTGAAGCAATCTAGCAA+TGG 0.648223 2:-23344915 None:intergenic
ATGTCGAATTAAGCAAACAA+TGG 0.648992 2:-23344810 None:intergenic
TATTAGCCACCATAAGGCGA+TGG 0.649019 2:-23344856 None:intergenic
AATGAAACTTGCAAATACAA+CGG 0.655557 2:+23345438 MsaT007829.1:CDS
GTATTGCAGGAAAGAAGCCA+TGG 0.665041 2:-23344512 None:intergenic
GCATCATCAAAAGAGAACCA+TGG 0.686492 2:+23344495 MsaT007829.1:CDS
AGAATCCGAATTTGTCGACG+AGG 0.706331 2:-23344352 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 23344251 23345501 23344251 ID=MsaG007829
Chr2 mRNA 23344251 23345501 23344251 ID=MsaT007829.1;Parent=MsaG007829
Chr2 exon 23344251 23345501 23344251 ID=MsaT007829.1.exon1;Parent=MsaT007829.1
Chr2 CDS 23344251 23345501 23344251 ID=cds.MsaT007829.1;Parent=MsaT007829.1
Gene Sequence

>MsaT007829.1

ATGTTGAAAGCATTTCTCTGTCGCAATGCTTTTGGCATACCAAATACTTCAACAACGCTCAGATTCCTTACCATCGCTTCACACCCTTTCGCTGTGTCCTACCTCGTCGACAAATTCGGATTCTCACATGAATTTGCACTCAAAGCTTCAGAATCAGAACACCTTCTTTTCAAAACCTCACAAAAACCAGATTCAGTTGTCAAATTCTTCAAAAATCACGGTTTCACTGACTCAGACATGAAAGGCATCATCAAAAGAGAACCATGGCTTCTTTCCTGCAATACCCACAAAACAATTCTCCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTGAATCTGACATTGTTCATATGGTTAGAGGTAACCCTAAGTTCTTGGAATTAAGCTTGAAGAATCATCAAATTAAGTTTGAATTTTTTCTATCCAAAGGTGCTTCTTCATCTGATATTGTTTCATTGTTAACTAAAAATCCTACAATTTTACACATTAGCTTGGAGAAACAAATTATCCCTCTTTTTGAATTGTTAAATAGGTTCTTCAAATCCAGTAAGGATACCATTGTTTGCTTAATTCGACATTCAACTACATTTGCTTTTAATTGCCATCGCCTTATGGTGGCTAATATCAATTTGATGATTGATTTTGAAGTTTCTGATTCTACCATTGCTAGATTGCTTCAAACTAGGCCATCTATATTTGGTTCAAAAGATTTGATTAAGTCATTAGAGGAAGTTAAGGGTTTAGGGTTTGATCCTTCATTGGCCACTTTTGGGGTTGCTTTGGTAGCCAAGAAATGTTTGAGTAAAAAACTTTGGGATGAGAAGGTTGACGACTTTAAGAAATGGGGTTGGTCTGATGAAGCTGTTATTCGAGTATTTAGGTGTCGTCCTAGTATTTTGTTGGTTTCCATTGAAAAGATTAATCTGTTGATGAGTTTTTGGGTTAATCAAATGGGTTGGGATTCCTTGGCACTTACTAAATGTCCACTTATGTTTTCTTATAGTTTACATAAAAGGATCATTCCAAGGGCCTTAGTTTTGCAATTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCAAGCTTAGTTATACCATTTACTTATTCTGAGAAGTTGTTCTTGAACAAGTTTGTTTTCAGCTTTAAGGAGGAGTCGGATTATCTGTTGAAGCTATATGACGAAAAAATGAAACTTGCAAATACAACGGAGAACAATGGGATGCCATCCACCAAATGTATAAGATTTTGA
Protein sequence

>MsaT007829.1

MLKAFLCRNAFGIPNTSTTLRFLTIASHPFAVSYLVDKFGFSHEFALKASESEHLLFKTSQKPDSVVKFFKNHGFTDSDMKGIIKREPWLLSCNTHKTILPKFQFLLSKGASESDIVHMVRGNPKFLELSLKNHQIKFEFFLSKGASSSDIVSLLTKNPTILHISLEKQIIPLFELLNRFFKSSKDTIVCLIRHSTTFAFNCHRLMVANINLMIDFEVSDSTIARLLQTRPSIFGSKDLIKSLEEVKGLGFDPSLATFGVALVAKKCLSKKLWDEKVDDFKKWGWSDEAVIRVFRCRPSILLVSIEKINLLMSFWVNQMGWDSLALTKCPLMFSYSLHKRIIPRALVLQFLLMKGLRKKNASLVIPFTYSEKLFLNKFVFSFKEESDYLLKLYDEKMKLANTTENNGMPSTKCIRF*