AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007878

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007878 MtrunA17_Chr2g0297191 91.376 1148 83 4 1 1147 1 1133 0.0 2105
MsaG007878 MtrunA17_Chr7g0221611 48.810 84 42 1 1045 1127 904 987 3.13e-17 87.8
MsaG007878 MtrunA17_Chr8g0347071 35.135 148 80 3 981 1127 755 887 3.66e-16 84.0
MsaG007878 MtrunA17_Chr1g0147121 45.349 86 46 1 1042 1127 888 972 1.82e-15 82.0
MsaG007878 MtrunA17_Chr3g0126701 48.148 81 41 1 1047 1127 922 1001 2.53e-15 81.3
MsaG007878 MtrunA17_Chr1g0149581 35.849 106 64 2 1046 1147 1521 1626 4.95e-14 77.4
MsaG007878 MtrunA17_Chr7g0275611 43.210 81 45 1 1047 1127 823 902 1.32e-13 75.9
MsaG007878 MtrunA17_Chr1g0201311 45.070 71 39 0 1058 1128 96 166 2.55e-12 70.9
MsaG007878 MtrunA17_Chr2g0305371 35.294 85 55 0 1044 1128 331 415 2.70e-12 70.9
MsaG007878 MtrunA17_Chr1g0201621 45.070 71 39 0 1058 1128 96 166 2.73e-12 70.5
MsaG007878 MtrunA17_Chr1g0149611 34.959 123 68 4 1017 1127 326 448 1.30e-11 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007878 AT5G42400.7 33.239 704 408 15 1 674 81 752 2.68e-92 325
MsaG007878 AT5G42400.7 50.479 313 144 5 837 1147 1047 1350 9.35e-74 269
MsaG007878 AT5G42400.2 33.239 704 408 15 1 674 81 752 2.68e-92 325
MsaG007878 AT5G42400.2 50.479 313 144 5 837 1147 1047 1350 9.35e-74 269
MsaG007878 AT5G42400.8 33.239 704 408 15 1 674 81 752 2.68e-92 325
MsaG007878 AT5G42400.8 50.479 313 144 5 837 1147 1047 1350 9.35e-74 269
MsaG007878 AT5G42400.5 33.239 704 408 15 1 674 81 752 2.68e-92 325
MsaG007878 AT5G42400.5 50.479 313 144 5 837 1147 1047 1350 9.35e-74 269
MsaG007878 AT5G42400.4 33.239 704 408 15 1 674 81 752 2.68e-92 325
MsaG007878 AT5G42400.4 50.479 313 144 5 837 1147 1047 1350 9.35e-74 269
MsaG007878 AT5G42400.1 33.239 704 408 15 1 674 81 752 2.68e-92 325
MsaG007878 AT5G42400.1 50.479 313 144 5 837 1147 1047 1350 9.35e-74 269
MsaG007878 AT5G42400.3 33.239 704 408 15 1 674 81 752 2.68e-92 325
MsaG007878 AT5G42400.3 50.479 313 144 5 837 1147 1047 1350 9.35e-74 269
MsaG007878 AT5G42400.6 33.239 704 408 15 1 674 81 752 2.68e-92 325
MsaG007878 AT5G42400.6 50.479 313 144 5 837 1147 1047 1350 9.35e-74 269
MsaG007878 AT1G05830.2 47.619 105 50 2 1025 1128 902 1002 6.40e-19 93.6
MsaG007878 AT1G05830.4 47.619 105 50 2 1025 1128 902 1002 6.40e-19 93.6
MsaG007878 AT1G05830.1 47.619 105 50 2 1025 1128 902 1002 6.40e-19 93.6
MsaG007878 AT1G05830.3 47.619 105 50 2 1025 1128 902 1002 6.40e-19 93.6
MsaG007878 AT2G31650.1 33.654 208 112 6 931 1127 788 980 1.32e-18 92.4
MsaG007878 AT4G27910.1 49.383 81 40 1 1047 1127 886 965 3.86e-16 84.3
MsaG007878 AT5G53430.1 48.780 82 41 1 1046 1127 901 981 8.72e-16 83.2
MsaG007878 AT1G76710.4 48.571 70 36 0 1058 1127 101 170 2.55e-14 77.8
MsaG007878 AT1G76710.3 48.571 70 36 0 1058 1127 101 170 2.69e-14 77.8
MsaG007878 AT1G76710.2 48.571 70 36 0 1058 1127 101 170 2.70e-14 77.4
MsaG007878 AT1G76710.1 48.571 70 36 0 1058 1127 101 170 2.70e-14 77.4
MsaG007878 AT3G61740.2 34.194 155 79 4 984 1127 777 919 9.97e-14 76.6
MsaG007878 AT3G61740.1 44.444 81 44 1 1047 1127 876 955 1.20e-13 76.3

Find 229 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGCATGTAACGTGTATT+TGG 0.173701 2:-23953805 None:intergenic
TCTCCTAATAACATCAAAAT+TGG 0.186129 2:+23948200 MsaT007878.1:CDS
TTGGCTTTATGAGGATAAAA+AGG 0.200293 2:+23946407 MsaT007878.1:CDS
AAGCCTGACCGTTCAAAATA+TGG 0.229760 2:-23947748 None:intergenic
GTGCTAACTTCGATACTTTC+TGG 0.237717 2:-23949543 None:intergenic
TTGCACGTTGAACAATAAAT+TGG 0.240841 2:+23947585 MsaT007878.1:CDS
GCGACATATTCTGTAATCTT+AGG 0.253086 2:-23948790 None:intergenic
CCTACCATTTAGTTTCTCTT+TGG 0.255660 2:-23949795 None:intergenic
TGATGTTATTAGGAGATAAA+AGG 0.264930 2:-23948193 None:intergenic
GGAAAGGCTAAGATGATTAA+AGG 0.273433 2:+23949430 MsaT007878.1:CDS
AGTGAAGGAAGGCACAAAAT+TGG 0.287240 2:+23949183 MsaT007878.1:CDS
TGCCTCAGTTACTTGTTTGC+TGG 0.289840 2:-23950153 None:intergenic
GACTTTGTCATTGAGTATAT+TGG 0.296267 2:+23950642 MsaT007878.1:CDS
AAGTTAAGGAAAGATGTTTC+TGG 0.308785 2:+23949361 MsaT007878.1:CDS
TGTTGAGCTTGCTTCTGTTA+TGG 0.312598 2:+23946164 MsaT007878.1:CDS
GATTTGTGTCCTGGATCTAT+TGG 0.316144 2:+23950206 MsaT007878.1:CDS
CCATTTCAGAGTTGATTTCC+TGG 0.317624 2:+23946451 MsaT007878.1:CDS
TCCCATCTAGCACAACTTTA+CGG 0.322868 2:-23947477 None:intergenic
TTGCTCCTCTTCTCAGGATA+TGG 0.329993 2:+23946122 MsaT007878.1:CDS
ATCTTCTACAACGGGCTTAG+AGG 0.331336 2:-23949308 None:intergenic
GTCTTTCTGCAAGAACAAAT+AGG 0.340439 2:+23950381 MsaT007878.1:CDS
AAGGAAGGCGCCGAAAGTTC+TGG 0.341237 2:+23949220 MsaT007878.1:CDS
TCATTACACCCATGTGCAAT+TGG 0.342636 2:-23947447 None:intergenic
ATCTCCAAAGAGAAACTAAA+TGG 0.343265 2:+23949791 MsaT007878.1:CDS
TCACTAAATATGTCTTTCTC+TGG 0.343385 2:-23948389 None:intergenic
TCCCGTAAAGTTGTGCTAGA+TGG 0.346597 2:+23947475 MsaT007878.1:CDS
TCTCTGGGGAAGGAATTAAT+TGG 0.347161 2:-23948373 None:intergenic
TGTGAAACAGACTTTGAATC+AGG 0.348335 2:-23946195 None:intergenic
TTGACAGATGATTGAGGCTT+AGG 0.356307 2:-23947962 None:intergenic
CCCATCTAGCACAACTTTAC+GGG 0.362728 2:-23947476 None:intergenic
TTCTGCTAAATCCAAGTCAT+TGG 0.364175 2:+23950184 MsaT007878.1:CDS
CCTGGAGGAAGAAGAAATAT+TGG 0.364781 2:+23947933 MsaT007878.1:CDS
GTTGTGCTAGATGGGATTAT+TGG 0.365764 2:+23947484 MsaT007878.1:CDS
CATTTGAATGATGCTACTTT+GGG 0.377631 2:+23949118 MsaT007878.1:CDS
TGAGCTTAAATCCACGTGAT+TGG 0.379621 2:-23946247 None:intergenic
ATCACCAACTCCATTGCTTT+GGG 0.382446 2:-23947383 None:intergenic
GCATTTGAATGATGCTACTT+TGG 0.382771 2:+23949117 MsaT007878.1:CDS
CATCACCAACTCCATTGCTT+TGG 0.389205 2:-23947384 None:intergenic
ACATCGCTGTTAACATTTGA+TGG 0.391242 2:-23945781 None:intergenic
ATCTCAGTTGTCAAATAGTA+AGG 0.392242 2:+23950358 MsaT007878.1:CDS
ATGGATGGGAATGGCATAAA+TGG 0.394098 2:+23950249 MsaT007878.1:CDS
ACAAAGTCCTCTGCTTCAAT+TGG 0.394100 2:-23950627 None:intergenic
TAAAGGAACTCCAGAACTTT+CGG 0.398697 2:-23949230 None:intergenic
TCTGAAAGTGCCTCAATTGA+AGG 0.399512 2:+23950448 MsaT007878.1:CDS
CAATATGAGAAGATGGGAAT+TGG 0.406771 2:+23951345 MsaT007878.1:CDS
TGACAAAATCTTGCGGTGAT+AGG 0.407599 2:-23949269 None:intergenic
TCAGCATGTAACGTGTATTT+GGG 0.407901 2:-23953804 None:intergenic
CCACAAGTAGAGCACGTGTC+AGG 0.416866 2:+23950288 MsaT007878.1:CDS
CGACTGGGGTCTTGTTGCCT+TGG 0.419851 2:+23950602 MsaT007878.1:CDS
CCCGTTGTAGAAGATAATTC+AGG 0.419889 2:+23949316 MsaT007878.1:CDS
CCAGGAAATCAACTCTGAAA+TGG 0.421721 2:-23946451 None:intergenic
GACAAATTGCTCCTCTTCTC+AGG 0.422116 2:+23946116 MsaT007878.1:CDS
TGTTCGAAGTGTTGTTGAAA+AGG 0.422666 2:+23948343 MsaT007878.1:CDS
CGTATCTCAGAGATGGTTGC+TGG 0.428715 2:+23948508 MsaT007878.1:CDS
CATTAAGGAGCAGTTATATG+AGG 0.431991 2:+23945933 MsaT007878.1:CDS
AAGTCATTGGATTTGTGTCC+TGG 0.434186 2:+23950197 MsaT007878.1:CDS
GTGAAGGAAGGCACAAAATT+GGG 0.441161 2:+23949184 MsaT007878.1:CDS
TTACATTCAACTGTTCTTGA+AGG 0.443516 2:-23948766 None:intergenic
CCAATATTTCTTCTTCCTCC+AGG 0.444169 2:-23947933 None:intergenic
CTTCAAGGATAATTGATCAC+TGG 0.445488 2:-23949509 None:intergenic
AGGTCCAGGGAAGGAGCCTT+TGG 0.446348 2:+23949336 MsaT007878.1:CDS
AGAATGCTGTTGGCTTTATG+AGG 0.446895 2:+23946398 MsaT007878.1:CDS
TTACCCCATGACCACGAGAC+TGG 0.447116 2:-23948927 None:intergenic
TTTATGTACAGTTGGCTCTC+AGG 0.447272 2:+23948090 MsaT007878.1:intron
TCAACGAATGTTAAGTCAGT+TGG 0.448542 2:+23947794 MsaT007878.1:CDS
CACTAAATATGTCTTTCTCT+GGG 0.451270 2:-23948388 None:intergenic
AGGAGCCTTTGGCTAAGTTA+AGG 0.452827 2:+23949347 MsaT007878.1:CDS
CCTGAATTATCTTCTACAAC+GGG 0.456131 2:-23949316 None:intergenic
CCCGTAAAGTTGTGCTAGAT+GGG 0.460102 2:+23947476 MsaT007878.1:CDS
CGGGATAATTACTTCTCAAA+TGG 0.461255 2:+23946281 MsaT007878.1:CDS
TAAGATGATTAAAGGGAAGA+AGG 0.462336 2:+23949438 MsaT007878.1:CDS
GGATACACAGGAAGCTCAAA+TGG 0.462764 2:-23945976 None:intergenic
AGCTGGGAGGAGAATGCTGT+TGG 0.463516 2:+23946388 MsaT007878.1:intron
CTGTTATTCAGACGATGTTA+CGG 0.463675 2:+23949652 MsaT007878.1:intron
TCTTTCTGCAAGAACAAATA+GGG 0.465926 2:+23950382 MsaT007878.1:CDS
AATTTCCAGTCTCGTGGTCA+TGG 0.467079 2:+23948922 MsaT007878.1:CDS
ATATAACTGCTCCTTAATGT+AGG 0.467895 2:-23945929 None:intergenic
CATTACACCCATGTGCAATT+GGG 0.471721 2:-23947446 None:intergenic
TCTGGAGTTCCTTTAGCGAT+TGG 0.472232 2:+23949238 MsaT007878.1:CDS
GAAAGGCTAAGATGATTAAA+GGG 0.477085 2:+23949431 MsaT007878.1:CDS
CTGACACGTGCTCTACTTGT+GGG 0.479131 2:-23950287 None:intergenic
TTAGCCAAAGGCTCCTTCCC+TGG 0.479653 2:-23949340 None:intergenic
AATGATGCTACTTTGGGACT+AGG 0.479668 2:+23949124 MsaT007878.1:CDS
CCTGACACGTGCTCTACTTG+TGG 0.480399 2:-23950288 None:intergenic
TGAGAGGATGTAACACCATC+TGG 0.480990 2:-23950083 None:intergenic
TGGAGAATTAATTCGTCCTC+GGG 0.481722 2:+23950662 MsaT007878.1:CDS
CTCTGCTTCAATTGGTTCCA+AGG 0.483937 2:-23950619 None:intergenic
CATTTCTTCCCAATTGCACA+TGG 0.484082 2:+23947438 MsaT007878.1:CDS
TGAGCTTCAATATGAGAAGA+TGG 0.485383 2:+23951338 MsaT007878.1:CDS
TCTTGCTGCAGCTGAGGGTG+CGG 0.485924 2:+23950421 MsaT007878.1:CDS
ATTGGGACTAGGAAAAGTTA+AGG 0.486627 2:+23949201 MsaT007878.1:CDS
CCTGTGTATCCTGTCATCAA+TGG 0.486921 2:+23945988 MsaT007878.1:CDS
TCTTCTCAGGATATGGTACA+TGG 0.487169 2:+23946129 MsaT007878.1:CDS
TTACTATTTGACAACTGAGA+TGG 0.489885 2:-23950356 None:intergenic
AAGCTCAAATGGCAAGAATC+CGG 0.491377 2:-23945965 None:intergenic
TCTTCAATCACCTTCAATTG+AGG 0.493337 2:-23950458 None:intergenic
TTCAAGGATAATTGATCACT+GGG 0.494291 2:-23949508 None:intergenic
GTAGAAGATAATTCAGGTCC+AGG 0.494526 2:+23949322 MsaT007878.1:CDS
TGTGCTAGGACTTCTATTGA+TGG 0.499906 2:+23950230 MsaT007878.1:CDS
AAGATATCCATGGTGGTGCC+AGG 0.501577 2:-23946469 None:intergenic
CACTCACACCAGAGTTTCCG+TGG 0.501812 2:+23945838 MsaT007878.1:CDS
CAGACTCGATGACGGTTATG+TGG 0.501932 2:+23951383 MsaT007878.1:CDS
ATTATTGTGCCATTGATGAC+AGG 0.502716 2:-23945997 None:intergenic
TTATATGAGGGTTTAACTAC+CGG 0.503467 2:+23945946 MsaT007878.1:CDS
AACCGTCATCGAGTCTGAAA+AGG 0.508500 2:-23951377 None:intergenic
GTATACTTTCCAATCGCTAA+AGG 0.508619 2:-23949247 None:intergenic
AACCCGGTGGAAACATGGTC+AGG 0.510121 2:-23946054 None:intergenic
AAATGACGTGGTTGATGAAG+AGG 0.513225 2:+23948669 MsaT007878.1:CDS
ATTTCCAGTCTCGTGGTCAT+GGG 0.513236 2:+23948923 MsaT007878.1:CDS
TAACCTTCTTGCTGCAGCTG+AGG 0.516247 2:+23950415 MsaT007878.1:CDS
CTCTTTGGAGATATGACCAG+TGG 0.516434 2:-23949780 None:intergenic
TTGGAGAATTAATTCGTCCT+CGG 0.516910 2:+23950661 MsaT007878.1:CDS
AGCCTGACCGTTCAAAATAT+GGG 0.519429 2:-23947747 None:intergenic
AATGTGCGGTCCCTACATTA+AGG 0.519889 2:+23945918 MsaT007878.1:CDS
CAAAACCTTTGCGGGATGAG+AGG 0.521228 2:-23950099 None:intergenic
AGGTGCAGACTCACTTGGAT+TGG 0.522409 2:-23947723 None:intergenic
GTTGATGCTGATAACCAGTC+TGG 0.526674 2:+23948134 MsaT007878.1:CDS
ATGATGAAGTTCTAAAAGAC+TGG 0.526710 2:+23948839 MsaT007878.1:CDS
AACCTTCTTGCTGCAGCTGA+GGG 0.528246 2:+23950416 MsaT007878.1:CDS
ATGCAAACCCGGTGGAAACA+TGG 0.528796 2:-23946059 None:intergenic
CGGGTTTGCATATTTGAGCA+TGG 0.532298 2:+23946071 MsaT007878.1:CDS
GCACAAAGCCACGGAAACTC+TGG 0.533762 2:-23945846 None:intergenic
AAAATTGGAAATACAGCAGA+AGG 0.535404 2:+23948215 MsaT007878.1:CDS
TATCCTGACCATGTTTCCAC+CGG 0.536239 2:+23946051 MsaT007878.1:CDS
ATCATTCCTTGCAAGTCATG+TGG 0.540334 2:+23947870 MsaT007878.1:CDS
CATCGCTGTTAACATTTGAT+GGG 0.543779 2:-23945780 None:intergenic
TATAACTGCTCCTTAATGTA+GGG 0.545813 2:-23945928 None:intergenic
TGGATGTATGTGAATGAACT+TGG 0.545855 2:+23945892 MsaT007878.1:CDS
TAGGACTTCTATTGATGGAT+GGG 0.547014 2:+23950235 MsaT007878.1:CDS
CACCCTCAGCTGCAGCAAGA+AGG 0.548860 2:-23950418 None:intergenic
CTAGGACTTCTATTGATGGA+TGG 0.550118 2:+23950234 MsaT007878.1:CDS
GAGATTGATGACCTACCACC+TGG 0.551551 2:+23948691 MsaT007878.1:CDS
AAAGTAACTGCTACTAAATG+TGG 0.552941 2:+23949409 MsaT007878.1:CDS
TGGGAAGGTGCAGACTCACT+TGG 0.553963 2:-23947728 None:intergenic
CACAAGTAGAGCACGTGTCA+GGG 0.556561 2:+23950289 MsaT007878.1:CDS
ATATGGTACATGGGCAAGAT+CGG 0.558546 2:+23946139 MsaT007878.1:CDS
ATTAAGGAGCAGTTATATGA+GGG 0.558740 2:+23945934 MsaT007878.1:CDS
GAAAGCAACACAGTGGGACT+AGG 0.559160 2:+23949157 MsaT007878.1:CDS
TCTTTCCTTAACTTAGCCAA+AGG 0.562647 2:-23949352 None:intergenic
GATTCATTGACAGATGATTG+AGG 0.563560 2:-23947968 None:intergenic
CTAGCACATCCAATAGATCC+AGG 0.565481 2:-23950215 None:intergenic
GAAGTCGTTCAGTGCAAACA+AGG 0.566037 2:+23949093 MsaT007878.1:CDS
CTGTCGCAATAACTCCAGAC+TGG 0.566459 2:-23948148 None:intergenic
GCATAAGTACCTCACAAGAA+TGG 0.567214 2:-23952197 None:intergenic
AAAGCATTTCAAGAATTGTG+TGG 0.567585 2:+23948640 MsaT007878.1:CDS
TAAGCTCAGAGGCATTCAGC+GGG 0.569394 2:+23946262 MsaT007878.1:CDS
CTGGATCTATTGGATGTGCT+AGG 0.569470 2:+23950216 MsaT007878.1:CDS
AATATGTCTTTCTCTGGGGA+AGG 0.571769 2:-23948383 None:intergenic
GACACGCATTCTTGAAAGTG+TGG 0.572215 2:+23948277 MsaT007878.1:CDS
AACTGCTACTAAATGTGGAA+AGG 0.572985 2:+23949414 MsaT007878.1:CDS
ACCTGAATTATCTTCTACAA+CGG 0.574706 2:-23949317 None:intergenic
CTGGTTATCAGCATCAACAT+TGG 0.574908 2:-23948129 None:intergenic
GACTTAACATTCGTTGAAGA+CGG 0.575920 2:-23947788 None:intergenic
GAAGGCACAAAATTGGGACT+AGG 0.577142 2:+23949190 MsaT007878.1:CDS
AGGACACAAATCCAATGACT+TGG 0.578633 2:-23950195 None:intergenic
CAGCAACCATCTCTGAGATA+CGG 0.578990 2:-23948507 None:intergenic
TTAGGAGATAAAAGGAACTC+AGG 0.580174 2:-23948185 None:intergenic
TGATAATAGTGTTGGCATGA+AGG 0.580374 2:+23949715 MsaT007878.1:CDS
TACTGTTTAAAATAATTCAA+CGG 0.581012 2:-23946030 None:intergenic
TTGGGAATCTGATCCACAGA+TGG 0.583030 2:-23947365 None:intergenic
AGATAATTCAGGTCCAGGGA+AGG 0.585334 2:+23949327 MsaT007878.1:CDS
ATTTATTAACCATTCTTGTG+AGG 0.586731 2:+23952188 MsaT007878.1:CDS
ACATGTCAATGACTGCACTA+AGG 0.587569 2:+23946221 MsaT007878.1:CDS
CATGCTCAAATATGCAAACC+CGG 0.588163 2:-23946070 None:intergenic
AGAAGGATACATCTTCTAAG+AGG 0.588713 2:+23949455 MsaT007878.1:CDS
GATTCCCAAAGCAATGGAGT+TGG 0.589499 2:+23947379 MsaT007878.1:CDS
GAGCTTCAATATGAGAAGAT+GGG 0.589585 2:+23951339 MsaT007878.1:CDS
TGAGCTTCACTGCTCTGCAA+AGG 0.589761 2:+23948304 MsaT007878.1:CDS
AAAAGAAAGCATCAGCCAGA+TGG 0.590108 2:+23950068 MsaT007878.1:CDS
ACTCTTACTGTATACTACAC+TGG 0.591630 2:+23953837 MsaT007878.1:CDS
CTTGGAACCAATTGAAGCAG+AGG 0.593149 2:+23950620 MsaT007878.1:CDS
ATGTACCATATCCTGAGAAG+AGG 0.596505 2:-23946127 None:intergenic
GCGTCAACTGATAATAGTGT+TGG 0.597250 2:+23949707 MsaT007878.1:CDS
TACATCCTCTCATCCCGCAA+AGG 0.597566 2:+23950094 MsaT007878.1:CDS
AAAGGAGCAAGATACACGAC+TGG 0.597716 2:+23950586 MsaT007878.1:CDS
TTGAATCTTCAAGATATCCA+TGG 0.599943 2:-23946479 None:intergenic
TGCATTCCACATGACTTGCA+AGG 0.600696 2:-23947876 None:intergenic
CTTCTATTGATGGATGGGAA+TGG 0.601171 2:+23950240 MsaT007878.1:CDS
GCATGGATTTCTCTAACGCA+AGG 0.602451 2:+23946088 MsaT007878.1:CDS
AGCATCAACATTGGATACAG+AGG 0.603476 2:-23948120 None:intergenic
GGATCAGATTCCCAAAGCAA+TGG 0.603808 2:+23947373 MsaT007878.1:CDS
GTGAAACAGACTTTGAATCA+GGG 0.603849 2:-23946194 None:intergenic
CAGGCTGTTGCACAAAGCCA+CGG 0.610390 2:-23945855 None:intergenic
GATACATGTGGAACCATCTG+TGG 0.612739 2:+23947352 MsaT007878.1:CDS
GTTGATGCTACAAAGAGAGG+TGG 0.614494 2:+23952156 MsaT007878.1:CDS
CACTAAGGAATCCAATCACG+TGG 0.615237 2:+23946236 MsaT007878.1:CDS
TGACTGCCGTATCTCAGAGA+TGG 0.615938 2:+23948501 MsaT007878.1:CDS
TTGATTTCCTGGCACCACCA+TGG 0.620952 2:+23946462 MsaT007878.1:CDS
AAGAAGAATTTCCAGTCTCG+TGG 0.621984 2:+23948916 MsaT007878.1:CDS
ACCATTCAGAGGAAAACCAC+TGG 0.628594 2:+23949764 MsaT007878.1:CDS
TAGAAGATAATTCAGGTCCA+GGG 0.630217 2:+23949323 MsaT007878.1:CDS
TCTCAGTTGTCAAATAGTAA+GGG 0.632048 2:+23950359 MsaT007878.1:CDS
TTGATGCTACAAAGAGAGGT+GGG 0.635479 2:+23952157 MsaT007878.1:CDS
CTTTCCGTGACAAAATCTTG+CGG 0.638552 2:-23949276 None:intergenic
AGAGAAAGACATATTTAGTG+AGG 0.639055 2:+23948391 MsaT007878.1:CDS
ACAAGTAGAGCACGTGTCAG+GGG 0.639473 2:+23950290 MsaT007878.1:CDS
TCTGCTGTAAATGCAATGAA+AGG 0.640153 2:+23947328 MsaT007878.1:CDS
ATCAACTCTGAAATGGAATG+TGG 0.641513 2:-23946444 None:intergenic
AACTGTGCTAGTAGAACACA+CGG 0.642305 2:-23945806 None:intergenic
TGACCGTTCAAAATATGGGA+AGG 0.642758 2:-23947743 None:intergenic
ATCCTGACCATGTTTCCACC+GGG 0.643417 2:+23946052 MsaT007878.1:CDS
GTGAAGGAAAGCAACACAGT+GGG 0.643882 2:+23949151 MsaT007878.1:CDS
ATTTCTTCCCAATTGCACAT+GGG 0.645487 2:+23947439 MsaT007878.1:CDS
GGACTAGGAAAAGTTAAGGA+AGG 0.646496 2:+23949205 MsaT007878.1:CDS
TTTCCAGTCTCGTGGTCATG+GGG 0.647882 2:+23948924 MsaT007878.1:CDS
CTTCTCAGGATATGGTACAT+GGG 0.650253 2:+23946130 MsaT007878.1:CDS
GGACTAGGAAAAGTGAAGGA+AGG 0.653313 2:+23949172 MsaT007878.1:CDS
AAGGAGCAAGATACACGACT+GGG 0.653984 2:+23950587 MsaT007878.1:CDS
TTAAGCTCAGAGGCATTCAG+CGG 0.654238 2:+23946261 MsaT007878.1:CDS
TACAGCAGACAGCAACACAA+AGG 0.655940 2:-23947314 None:intergenic
ACACTGATGTCACCATTCAG+AGG 0.656090 2:+23949753 MsaT007878.1:CDS
TTTGGGACTAGGAAAAGTGA+AGG 0.658589 2:+23949135 MsaT007878.1:CDS
GTGTGGACATTTAAATGACG+TGG 0.659997 2:+23948657 MsaT007878.1:CDS
GCAAACAAGTAACTGAGGCA+CGG 0.664806 2:+23950156 MsaT007878.1:CDS
ACTAAATATGTCTTTCTCTG+GGG 0.667298 2:-23948387 None:intergenic
TAGGTTGATGCTACAAAGAG+AGG 0.676637 2:+23952153 MsaT007878.1:intron
CCATTGATGACAGGATACAC+AGG 0.685505 2:-23945988 None:intergenic
TCACGTGGATTTAAGCTCAG+AGG 0.688485 2:+23946251 MsaT007878.1:CDS
AGTGGGACTAGGAAAAGTGA+AGG 0.694009 2:+23949168 MsaT007878.1:CDS
AGCCAGCAAACAAGTAACTG+AGG 0.695660 2:+23950151 MsaT007878.1:CDS
GCAATGAAAGGAGATACATG+TGG 0.714311 2:+23947340 MsaT007878.1:CDS
TAATAGTGTTGGCATGAAGG+TGG 0.720324 2:+23949718 MsaT007878.1:CDS
AACAAGTAACTGAGGCACGG+AGG 0.728589 2:+23950159 MsaT007878.1:CDS
AATCTTCAAGATATCCATGG+TGG 0.734776 2:-23946476 None:intergenic
GCTCAAATATGCAAACCCGG+TGG 0.741563 2:-23946067 None:intergenic
AATGAACTTGGACAAATGTG+CGG 0.741964 2:+23945904 MsaT007878.1:CDS
AGTGAAGGAAAGCAACACAG+TGG 0.746407 2:+23949150 MsaT007878.1:CDS
AGGAGCAAGATACACGACTG+GGG 0.750387 2:+23950588 MsaT007878.1:CDS
ATATCTTGAAGATTCAACAG+TGG 0.754206 2:+23946485 MsaT007878.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 23945766 23953863 23945766 ID=MsaG007878
Chr2 mRNA 23945766 23953863 23945766 ID=MsaT007878.1;Parent=MsaG007878
Chr2 exon 23945766 23946302 23945766 ID=MsaT007878.1.exon1;Parent=MsaT007878.1
Chr2 CDS 23945766 23946302 23945766 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23946390 23946506 23946390 ID=MsaT007878.1.exon2;Parent=MsaT007878.1
Chr2 CDS 23946390 23946506 23946390 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23947286 23947606 23947286 ID=MsaT007878.1.exon3;Parent=MsaT007878.1
Chr2 CDS 23947286 23947606 23947286 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23947707 23948024 23947707 ID=MsaT007878.1.exon4;Parent=MsaT007878.1
Chr2 CDS 23947707 23948024 23947707 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23948101 23948412 23948101 ID=MsaT007878.1.exon5;Parent=MsaT007878.1
Chr2 CDS 23948101 23948412 23948101 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23948490 23948945 23948490 ID=MsaT007878.1.exon6;Parent=MsaT007878.1
Chr2 CDS 23948490 23948945 23948490 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23949088 23949577 23949088 ID=MsaT007878.1.exon7;Parent=MsaT007878.1
Chr2 CDS 23949088 23949577 23949088 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23949663 23949812 23949663 ID=MsaT007878.1.exon8;Parent=MsaT007878.1
Chr2 CDS 23949663 23949812 23949663 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23950045 23950469 23950045 ID=MsaT007878.1.exon9;Parent=MsaT007878.1
Chr2 CDS 23950045 23950469 23950045 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23950561 23950683 23950561 ID=MsaT007878.1.exon10;Parent=MsaT007878.1
Chr2 CDS 23950561 23950683 23950561 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23951324 23951404 23951324 ID=MsaT007878.1.exon11;Parent=MsaT007878.1
Chr2 CDS 23951324 23951404 23951324 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23952156 23952209 23952156 ID=MsaT007878.1.exon12;Parent=MsaT007878.1
Chr2 CDS 23952156 23952209 23952156 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Chr2 exon 23953795 23953863 23953795 ID=MsaT007878.1.exon13;Parent=MsaT007878.1
Chr2 CDS 23953795 23953863 23953795 ID=cds.MsaT007878.1;Parent=MsaT007878.1
Gene Sequence

>MsaT007878.1

ATGGAGTTGAGCTGCCCATCAAATGTTAACAGCGATGTTTCCGTGTGTTCTACTAGCACAGTTAATATTTCACACTCACACCAGAGTTTCCGTGGCTTTGTGCAACAGCCTGTTTTTGTCAGTGGATGGATGTATGTGAATGAACTTGGACAAATGTGCGGTCCCTACATTAAGGAGCAGTTATATGAGGGTTTAACTACCGGATTCTTGCCATTTGAGCTTCCTGTGTATCCTGTCATCAATGGCACAATAATGAACTCTGTACCGTTGAATTATTTTAAACAGTATCCTGACCATGTTTCCACCGGGTTTGCATATTTGAGCATGGATTTCTCTAACGCAAGGATGTCGACAAATTGCTCCTCTTCTCAGGATATGGTACATGGGCAAGATCGGTCTGTTGAGCTTGCTTCTGTTATGGCTGTTGACCCTGATTCAAAGTCTGTTTCACAATCACATGTCAATGACTGCACTAAGGAATCCAATCACGTGGATTTAAGCTCAGAGGCATTCAGCGGGATAATTACTTCTCAAATGCTGGGAGGAGAATGCTGTTGGCTTTATGAGGATAAAAAGGGTATGAAACATGGGCCACATTCCATTTCAGAGTTGATTTCCTGGCACCACCATGGATATCTTGAAGATTCAACAGTGATCTCTCATTTTGACAATAAGTATGGAACCTTTGTGTTGCTGTCTGCTGTAAATGCAATGAAAGGAGATACATGTGGAACCATCTGTGGATCAGATTCCCAAAGCAATGGAGTTGGTGATGTCATGAACTTAATTTGTGAAATATCTGAAGACATTTCTTCCCAATTGCACATGGGTGTAATGAAAGCTTCCCGTAAAGTTGTGCTAGATGGGATTATTGGCGATATCATTGCAGAGTTTATTACTGAAAAGAAATCCAAGAAGCAAAAACTTGAGTCTGCTGATCAAACTTCTGAAACTTGCACGTTGAACAATAAATTGATGAATAAAGGTGCTTCCAATCCAAGTGAGTCTGCACCTTCCCATATTTTGAACGGTCAGGCTTGTCATGAAATTTCTAGACCGTCTTCAACGAATGTTAAGTCAGTTGGAAGCATTGAAAATTTTTGGTGGTCATATGCTGTTGTTCGTAAAGTACTTTTTGATCATTCCTTGCAAGTCATGTGGAATGCAGTGTTTTTTGACACGGTGACAGAGTTCTTATTTTCCTGGAGGAAGAAGAAATATTGGTCTCATCCTAAGCCTCAATCATCTGTCAATGAATCTAAAGATTCTGTTGAAAAGTTAAAATCAGAAGCTTTGGCTCTCAGGACAAGTTCCTCTGTATCCAATGTTGATGCTGATAACCAGTCTGGAGTTATTGCGACAGAAAGAGATTGTCATCCTGAGTTCCTTTTATCTCCTAATAACATCAAAATTGGAAATACAGCAGAAGGTCAAAGAGTTTCATGCAGTTATGATAACTCTGAAGATTTGACACGCATTCTTGAAAGTGTGGAGAATGAGCTTCACTGCTCTGCAAAGGTGTCTTTAGCTGATTATGTTCGAAGTGTTGTTGAAAAGGAAGTAAACCAATTAATTCCTTCCCCAGAGAAAGACATATTTAGTGAGGTTGATGTTAATGACTGCCGTATCTCAGAGATGGTTGCTGGTAAAACATCTGTTAAAGAAACTCTAAGTGACAAGTCTATCGATCCTGTGAAAAATGGAGACTCAATTTGTATGCCTTCATCTGAAAATCATATGTCCAATGTATTCTCAAAAGCATTTCAAGAATTGTGTGGACATTTAAATGACGTGGTTGATGAAGAGGAGATTGATGACCTACCACCTGGATTCGAAAAGAATTCTCAAACAATATTCCCACACTGCAATTCAAAATTTCGACCTTCAAGAACAGTTGAATGTAATCCTAAGATTACAGAATATGTCGCAGCTGCACTGTGCAGACAGAAGCTGCATGATGAAGTTCTAAAAGACTGGAAGCTGTCGATTCTTGATTCTACATTTAAACAAGTTATGTCATCATGTACTAAAAAGAAGAATTTCCAGTCTCGTGGTCATGGGAAGGGGAAGTCGTTCAGTGCAAACAAGGAGCATTTGAATGATGCTACTTTGGGACTAGGAAAAGTGAAGGAAAGCAACACAGTGGGACTAGGAAAAGTGAAGGAAGGCACAAAATTGGGACTAGGAAAAGTTAAGGAAGGCGCCGAAAGTTCTGGAGTTCCTTTAGCGATTGGAAAGTATACCTATCACCGCAAGATTTTGTCACGGAAAGAGCTTTGCTCCTCTAAGCCCGTTGTAGAAGATAATTCAGGTCCAGGGAAGGAGCCTTTGGCTAAGTTAAGGAAAGATGTTTCTGGAGATGTGAATGAGAGTGCAGAACTTAAAGTAACTGCTACTAAATGTGGAAAGGCTAAGATGATTAAAGGGAAGAAGGATACATCTTCTAAGAGGAGCTCTTCTGTCAATGTCGATAATAGTTCACCCAGTGATCAATTATCCTTGAAGAATAAAACTAGCCAGAAAGTATCGAAGTTAGCACATACAGTTCAAAACGATGTTACGGATGTTGCGAAGTCTAATAAAAAGAGGCTCTTGGCGTCAACTGATAATAGTGTTGGCATGAAGGTGGTTAAAAGAAATAACACTGATGTCACCATTCAGAGGAAAACCACTGGTCATATCTCCAAAGAGAAACTAAATGCGACAAATACAGTATCAAAATCAAAAAGAAAGCATCAGCCAGATGGTGTTACATCCTCTCATCCCGCAAAGGTTTTGAAAATTTCAAAAAGCAGTGCAAGTCTGGGAGCCAGCAAACAAGTAACTGAGGCACGGAGGAATTCTGCTAAATCCAAGTCATTGGATTTGTGTCCTGGATCTATTGGATGTGCTAGGACTTCTATTGATGGATGGGAATGGCATAAATGGTCTCAAAGTGCTAGTCCCACAAGTAGAGCACGTGTCAGGGGTCTTCCGCGTTTACAAAACAAGTACATAAATTCTGAAAATAATCCATCTCAGTTGTCAAATAGTAAGGGTCTTTCTGCAAGAACAAATAGGGTGAAGTTACGTAACCTTCTTGCTGCAGCTGAGGGTGCGGATCTTCTGAAAGTGCCTCAATTGAAGGCACGGAAAAAGCGACTACGTTTTCAAAGGAGCAAGATACACGACTGGGGTCTTGTTGCCTTGGAACCAATTGAAGCAGAGGACTTTGTCATTGAGTATATTGGAGAATTAATTCGTCCTCGGATATCTGATATACGTGAGCTTCAATATGAGAAGATGGGAATTGGAAGCAGTTACCTTTTCAGACTCGATGACGGTTATGTGGTTGATGCTACAAAGAGAGGTGGGATTGCACGATTTATTAACCATTCTTGTGAGGATTATACTCCCAAATACACGTTACATGCTGAAGATTTGAAGACTCTTACTGTATACTACACTGGATAG
Protein sequence

>MsaT007878.1

MELSCPSNVNSDVSVCSTSTVNISHSHQSFRGFVQQPVFVSGWMYVNELGQMCGPYIKEQLYEGLTTGFLPFELPVYPVINGTIMNSVPLNYFKQYPDHVSTGFAYLSMDFSNARMSTNCSSSQDMVHGQDRSVELASVMAVDPDSKSVSQSHVNDCTKESNHVDLSSEAFSGIITSQMLGGECCWLYEDKKGMKHGPHSISELISWHHHGYLEDSTVISHFDNKYGTFVLLSAVNAMKGDTCGTICGSDSQSNGVGDVMNLICEISEDISSQLHMGVMKASRKVVLDGIIGDIIAEFITEKKSKKQKLESADQTSETCTLNNKLMNKGASNPSESAPSHILNGQACHEISRPSSTNVKSVGSIENFWWSYAVVRKVLFDHSLQVMWNAVFFDTVTEFLFSWRKKKYWSHPKPQSSVNESKDSVEKLKSEALALRTSSSVSNVDADNQSGVIATERDCHPEFLLSPNNIKIGNTAEGQRVSCSYDNSEDLTRILESVENELHCSAKVSLADYVRSVVEKEVNQLIPSPEKDIFSEVDVNDCRISEMVAGKTSVKETLSDKSIDPVKNGDSICMPSSENHMSNVFSKAFQELCGHLNDVVDEEEIDDLPPGFEKNSQTIFPHCNSKFRPSRTVECNPKITEYVAAALCRQKLHDEVLKDWKLSILDSTFKQVMSSCTKKKNFQSRGHGKGKSFSANKEHLNDATLGLGKVKESNTVGLGKVKEGTKLGLGKVKEGAESSGVPLAIGKYTYHRKILSRKELCSSKPVVEDNSGPGKEPLAKLRKDVSGDVNESAELKVTATKCGKAKMIKGKKDTSSKRSSSVNVDNSSPSDQLSLKNKTSQKVSKLAHTVQNDVTDVAKSNKKRLLASTDNSVGMKVVKRNNTDVTIQRKTTGHISKEKLNATNTVSKSKRKHQPDGVTSSHPAKVLKISKSSASLGASKQVTEARRNSAKSKSLDLCPGSIGCARTSIDGWEWHKWSQSASPTSRARVRGLPRLQNKYINSENNPSQLSNSKGLSARTNRVKLRNLLAAAEGADLLKVPQLKARKKRLRFQRSKIHDWGLVALEPIEAEDFVIEYIGELIRPRISDIRELQYEKMGIGSSYLFRLDDGYVVDATKRGGIARFINHSCEDYTPKYTLHAEDLKTLTVYYTG*