AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007884


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007884 MtrunA17_Chr2g0297251 98.712 233 3 0 1 233 26 258 8.07e-176 483
MsaG007884 MtrunA17_Chr4g0065041 45.098 255 109 6 5 232 1 251 3.36e-62 195
MsaG007884 MtrunA17_Chr7g0230511 43.388 242 118 5 9 232 6 246 2.78e-58 184
MsaG007884 MtrunA17_Chr4g0040071 35.371 229 107 6 12 233 15 209 2.35e-39 135
MsaG007884 MtrunA17_Chr5g0395181 33.778 225 109 4 12 233 21 208 2.62e-38 132
MsaG007884 MtrunA17_Chr5g0411151 33.617 235 111 5 9 233 32 231 4.52e-37 130
MsaG007884 MtrunA17_Chr8g0366581 44.615 130 71 1 9 137 30 159 2.51e-36 128
MsaG007884 MtrunA17_Chr1g0179781 33.628 226 120 5 10 233 5 202 3.02e-36 127
MsaG007884 MtrunA17_Chr4g0052451 72.619 84 17 1 62 139 10 93 4.67e-36 122
MsaG007884 MtrunA17_Chr7g0265291 34.498 229 115 5 10 233 5 203 7.12e-35 123
MsaG007884 MtrunA17_Chr4g0052461 82.759 58 10 0 8 65 18 75 6.90e-32 111
MsaG007884 MtrunA17_Chr2g0320521 39.869 153 77 5 3 144 1 149 1.60e-26 102
MsaG007884 MtrunA17_Chr2g0320561 38.776 147 79 4 14 149 6 152 2.55e-25 99.8
MsaG007884 MtrunA17_Chr2g0320551 37.162 148 82 4 14 150 6 153 1.89e-23 92.8
MsaG007884 MtrunA17_Chr4g0020811 40.000 150 69 6 3 138 1 143 1.98e-23 92.4
MsaG007884 MtrunA17_Chr2g0308401 40.559 143 68 5 10 138 2 141 8.76e-23 90.5
MsaG007884 MtrunA17_Chr8g0392151 34.194 155 80 4 4 137 5 158 6.60e-21 87.8
MsaG007884 MtrunA17_Chr5g0434331 32.090 134 84 4 11 140 42 172 2.25e-15 72.4
MsaG007884 MtrunA17_Chr8g0386511 33.333 117 74 2 50 162 4 120 1.58e-14 68.6
MsaG007884 MtrunA17_Chr1g0161601 44.048 84 45 2 6 89 42 123 8.49e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007884 AT1G31040.1 61.905 231 75 5 11 233 18 243 2.90e-96 281
MsaG007884 AT3G60670.1 42.437 238 120 3 12 232 7 244 2.90e-57 182
MsaG007884 AT2G12646.3 40.392 255 117 4 10 232 1 252 1.23e-55 178
MsaG007884 AT2G12646.1 39.922 258 120 4 7 232 1 255 7.12e-55 176
MsaG007884 AT2G12646.2 39.922 258 120 4 7 232 1 255 7.12e-55 176
MsaG007884 AT1G43000.2 35.242 227 126 5 10 232 14 223 3.31e-37 130
MsaG007884 AT1G43000.1 35.242 227 126 5 10 232 7 216 4.04e-37 130
MsaG007884 AT1G21000.1 34.298 242 127 6 12 232 16 246 1.90e-36 129
MsaG007884 AT1G21000.2 34.298 242 127 6 12 232 13 243 2.10e-36 129
MsaG007884 AT2G01818.1 48.148 135 60 3 13 137 12 146 8.57e-35 124
MsaG007884 AT1G32700.1 45.669 127 68 1 12 137 12 138 1.86e-34 122
MsaG007884 AT4G17900.1 45.038 131 70 2 9 137 31 161 3.19e-34 122
MsaG007884 AT4G17900.2 45.038 131 70 2 9 137 17 147 7.23e-34 121
MsaG007884 AT1G76590.1 44.615 130 71 1 12 140 18 147 7.42e-34 122
MsaG007884 AT2G27930.1 44.681 141 67 3 11 140 3 143 1.61e-33 119
MsaG007884 AT2G27930.4 44.681 141 67 3 11 140 3 143 1.61e-33 119
MsaG007884 AT5G46710.2 44.286 140 76 2 5 142 21 160 1.87e-33 119
MsaG007884 AT2G27930.3 45.000 140 66 3 11 139 3 142 2.07e-33 119
MsaG007884 AT2G27930.2 45.000 140 66 3 11 139 3 142 2.07e-33 119
MsaG007884 AT5G46710.1 45.185 135 72 2 5 137 21 155 7.12e-33 119
MsaG007884 AT5G46710.3 45.802 131 69 2 9 137 9 139 1.35e-32 118
MsaG007884 AT1G32700.2 46.465 99 52 1 40 137 1 99 2.86e-25 98.2
MsaG007884 AT2G27930.5 45.455 88 39 1 62 140 59 146 8.92e-19 81.3
MsaG007884 AT2G27930.6 45.455 88 39 1 62 140 59 146 8.92e-19 81.3
MsaG007884 AT2G01818.2 49.351 77 33 2 67 137 6 82 1.33e-16 74.7

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTTGGATTTGTTCTTCT+TGG 0.221070 2:-24048853 None:intergenic
TGTTGCTGGTGGCCTCTTCT+TGG 0.257102 2:-24048824 None:intergenic
TTTGCACTGTTAATAGTATA+GGG 0.310183 2:-24042352 None:intergenic
CCTGGCATTGTCCTCTCTCT+TGG 0.334179 2:+24048882 MsaT007884.1:CDS
CACTTGTGACAGGATTCTTC+AGG 0.345103 2:+24042438 MsaT007884.1:CDS
CATTGATTGTTCTAATATAC+AGG 0.353712 2:+24041876 MsaT007884.1:CDS
TTGATGGATGAAACATTCTT+TGG 0.372131 2:+24037085 MsaT007884.1:CDS
TAGGATAGTGGGGCCCTTTG+AGG 0.381993 2:-24048924 None:intergenic
CGTAATTCGATTGGATGATC+TGG 0.385003 2:+24041846 MsaT007884.1:CDS
GTAATAATGGTTTAGGATAG+TGG 0.420638 2:-24048936 None:intergenic
CGTTCTTCCTTCGATTCTTG+TGG 0.423267 2:-24037120 None:intergenic
ATAAGTGCAGGTGGATTTCA+TGG 0.424549 2:+24048644 MsaT007884.1:intron
CGATTTGATGAATCTGACTT+AGG 0.432856 2:+24048702 MsaT007884.1:CDS
GGCCTCTTCTTGGTGAGTGA+TGG 0.433860 2:-24048814 None:intergenic
TGCATGAGAGAGAACAGAAA+TGG 0.450248 2:-24042468 None:intergenic
TGCTTCTCATCCTCTTCTCC+AGG 0.455215 2:+24037204 MsaT007884.1:CDS
GAAACATTCTTTGGTGATTG+TGG 0.465089 2:+24037094 MsaT007884.1:CDS
AAACATTCTTTGGTGATTGT+GGG 0.470651 2:+24037095 MsaT007884.1:CDS
TTACAACAACGTAATTCGAT+TGG 0.478043 2:+24041837 MsaT007884.1:CDS
CCAAGAGAGAGGACAATGCC+AGG 0.479103 2:-24048882 None:intergenic
ATGCTTGCTTCACTTGTGAC+AGG 0.483376 2:+24042428 MsaT007884.1:CDS
ATCAACCTCTCTAGCCATGC+AGG 0.486614 2:-24037067 None:intergenic
GCAATTCGAGGGACTAAGAA+TGG 0.489732 2:+24048731 MsaT007884.1:CDS
GAAGAAAAGGTGCTCCTCAA+AGG 0.491867 2:+24048910 MsaT007884.1:CDS
ATCAAATCGATGTAAAATGT+TGG 0.496431 2:-24048688 None:intergenic
TCTCTTGGAAGCAGAAGAAA+AGG 0.496815 2:+24048897 MsaT007884.1:CDS
ATGGAGTAATTAGAAGAACT+TGG 0.498349 2:-24048795 None:intergenic
TTTCTGTTCTCTCTCATGCA+AGG 0.498493 2:+24042471 MsaT007884.1:CDS
TAATAATGGTTTAGGATAGT+GGG 0.503746 2:-24048935 None:intergenic
GAGAGAGAACAGAAATGGAA+TGG 0.509323 2:-24042463 None:intergenic
AGAGAGAACAGAAATGGAAT+GGG 0.519403 2:-24042462 None:intergenic
AAGAAAAGGTGCTCCTCAAA+GGG 0.524805 2:+24048911 MsaT007884.1:CDS
ACTTTGCATGACTTTGATTG+TGG 0.529762 2:-24042397 None:intergenic
CTTAGGAATATCGCAATTCG+AGG 0.531509 2:+24048719 MsaT007884.1:CDS
TTCTTCTGCTTCCAAGAGAG+AGG 0.533226 2:-24048893 None:intergenic
TGAAACCTGCATGGCTAGAG+AGG 0.557873 2:+24037062 MsaT007884.1:CDS
TTCGAGGGACTAAGAATGGA+TGG 0.559888 2:+24048735 MsaT007884.1:CDS
AGCATGAGAAGATAAGCAGT+GGG 0.561028 2:-24037185 None:intergenic
TGCATGGCTAGAGAGGTTGA+TGG 0.563271 2:+24037069 MsaT007884.1:CDS
AATGCCAGGGAGGAATCTGT+TGG 0.577735 2:-24048868 None:intergenic
CTCCATCACTCACCAAGAAG+AGG 0.578593 2:+24048812 MsaT007884.1:CDS
TAATATAATACCTGGAGAAG+AGG 0.580127 2:-24037214 None:intergenic
GTCACAAGTGAAGCAAGCAT+TGG 0.580435 2:-24042425 None:intergenic
TCATGATGCTGTAGGTGAGA+AGG 0.582777 2:+24041809 MsaT007884.1:intron
ACTAAGAATGGATGGATCTG+AGG 0.607142 2:+24048743 MsaT007884.1:CDS
AAGCATGAGAAGATAAGCAG+TGG 0.608759 2:-24037186 None:intergenic
AACATTCTTTGGTGATTGTG+GGG 0.611739 2:+24037096 MsaT007884.1:CDS
TGGAGTAATTAGAAGAACTT+GGG 0.613997 2:-24048794 None:intergenic
GTGGGGTCCACAAGAATCGA+AGG 0.615814 2:+24037113 MsaT007884.1:CDS
GATGAAGATAACAACCACAT+TGG 0.616337 2:+24048771 MsaT007884.1:CDS
CATGAGAAGATAAGCAGTGG+GGG 0.623874 2:-24037183 None:intergenic
TAGAAGAACTTGGGCCAATG+TGG 0.628681 2:-24048785 None:intergenic
AAATCCAACAGATTCCTCCC+TGG 0.630961 2:+24048864 MsaT007884.1:CDS
CAAGAGAGAGGACAATGCCA+GGG 0.632298 2:-24048881 None:intergenic
TTAGGAATATCGCAATTCGA+GGG 0.644008 2:+24048720 MsaT007884.1:CDS
GAGAGAGGACAATGCCAGGG+AGG 0.651378 2:-24048878 None:intergenic
AATAATGGTTTAGGATAGTG+GGG 0.654932 2:-24048934 None:intergenic
GGTGGATTTCATGGTATACG+AGG 0.668694 2:+24048653 MsaT007884.1:intron
ATCAAGAGGAGACATGGACA+TGG 0.680821 2:+24037021 None:intergenic
TGATGAATATGAAACCTGCA+TGG 0.699453 2:+24037053 MsaT007884.1:CDS
GTGGATTTCATGGTATACGA+GGG 0.708036 2:+24048654 MsaT007884.1:CDS
GCATGAGAAGATAAGCAGTG+GGG 0.713738 2:-24037184 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 24037034 24048947 24037034 ID=MsaG007884
Chr2 mRNA 24037034 24048947 24037034 ID=MsaT007884.1;Parent=MsaG007884
Chr2 exon 24037034 24037225 24037034 ID=MsaT007884.1.exon1;Parent=MsaT007884.1
Chr2 CDS 24037034 24037225 24037034 ID=cds.MsaT007884.1;Parent=MsaT007884.1
Chr2 exon 24041823 24041897 24041823 ID=MsaT007884.1.exon2;Parent=MsaT007884.1
Chr2 CDS 24041823 24041897 24041823 ID=cds.MsaT007884.1;Parent=MsaT007884.1
Chr2 exon 24042352 24042492 24042352 ID=MsaT007884.1.exon3;Parent=MsaT007884.1
Chr2 CDS 24042352 24042492 24042352 ID=cds.MsaT007884.1;Parent=MsaT007884.1
Chr2 exon 24048654 24048947 24048654 ID=MsaT007884.1.exon4;Parent=MsaT007884.1
Chr2 CDS 24048654 24048947 24048654 ID=cds.MsaT007884.1;Parent=MsaT007884.1
Gene Sequence

>MsaT007884.1

ATGGACATGGACATGAGTATGATGAATATGAAACCTGCATGGCTAGAGAGGTTGATGGATGAAACATTCTTTGGTGATTGTGGGGTCCACAAGAATCGAAGGAAGAACGAGAAGAACATTTTTTGCTTACACTGTTGTCTTAGCATCTGCCCCCACTGCTTATCTTCTCATGCTTCTCATCCTCTTCTCCAGGTGAGAAGGTATGTTTACAACAACGTAATTCGATTGGATGATCTGGAAAAGCTCATTGATTGTTCTAATATACAGCCCTATACTATTAACAGTGCAAAAGTGATATTTTTAAATCAGAGGCCACAATCAAAGTCATGCAAAGTTACAACCAATGCTTGCTTCACTTGTGACAGGATTCTTCAGGACCCATTCCATTTCTGTTCTCTCTCATGCAAGGTGGATTTCATGGTATACGAGGGTCAAAGTTTGTCCAACATTTTACATCGATTTGATGAATCTGACTTAGGAATATCGCAATTCGAGGGACTAAGAATGGATGGATCTGAGGTAATTGATGAAGATAACAACCACATTGGCCCAAGTTCTTCTAATTACTCCATCACTCACCAAGAAGAGGCCACCAGCAACAAAATCACCAAGAAGAACAAATCCAACAGATTCCTCCCTGGCATTGTCCTCTCTCTTGGAAGCAGAAGAAAAGGTGCTCCTCAAAGGGCCCCACTATCCTAA

Protein sequence

>MsaT007884.1

MDMDMSMMNMKPAWLERLMDETFFGDCGVHKNRRKNEKNIFCLHCCLSICPHCLSSHASHPLLQVRRYVYNNVIRLDDLEKLIDCSNIQPYTINSAKVIFLNQRPQSKSCKVTTNACFTCDRILQDPFHFCSLSCKVDFMVYEGQSLSNILHRFDESDLGISQFEGLRMDGSEVIDEDNNHIGPSSSNYSITHQEEATSNKITKKNKSNRFLPGIVLSLGSRRKGAPQRAPLS*