AlfalfaGEDB | MsaG007931

M. sativa ssp. caerulea / MsaG007931

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007931	RHN73389.1	93.086	405	20	2	182	580	9	411	0.0	757

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007931	sp\|Q9FLV4\|Y5248_ARATH	49.329	298	134	6	227	515	515	804	1.83e-80	273

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007931	tr\|A0A396J5I7\|A0A396J5I7_MEDTR	93.086	405	20	2	182	580	9	411	0.0	757

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007931	MtrunA17_Chr2g0297911	93.086	405	20	2	182	580	9	411	0.0	757
MsaG007931	MtrunA17_Chr2g0297961	86.432	398	49	2	185	579	14	409	0.0	697
MsaG007931	MtrunA17_Chr2g0297901	65.470	362	74	2	217	577	1	312	2.51e-165	473
MsaG007931	MtrunA17_Chr2g0297921	50.498	402	184	4	184	574	76	473	1.68e-135	403
MsaG007931	MtrunA17_Chr2g0297951	55.331	347	152	2	206	550	97	442	4.57e-132	394
MsaG007931	MtrunA17_Chr2g0297941	48.968	339	154	7	229	550	65	401	1.32e-97	303
MsaG007931	MtrunA17_Chr1g0157811	44.767	344	173	8	229	562	187	523	1.62e-88	283
MsaG007931	MtrunA17_Chr1g0160681	44.304	316	164	6	217	525	40	350	8.09e-88	276
MsaG007931	MtrunA17_Chr1g0157951	43.220	354	188	6	217	562	156	504	2.02e-86	277
MsaG007931	MtrunA17_Chr3g0088321	47.797	295	142	6	229	517	40	328	3.07e-86	272

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007931	AT5G24080.3	49.329	298	134	6	227	515	388	677	2.35e-82	273
MsaG007931	AT5G24080.1	49.329	298	134	6	227	515	388	677	2.35e-82	273
MsaG007931	AT5G24080.2	49.329	298	134	6	227	515	515	804	1.87e-81	273
MsaG007931	AT1G34300.1	42.450	351	186	4	211	557	453	791	4.25e-80	269
MsaG007931	AT4G32300.1	42.493	353	177	8	218	551	470	815	2.12e-77	261
MsaG007931	AT5G38240.3	43.151	292	153	5	231	517	260	543	7.03e-76	252
MsaG007931	AT5G38240.2	43.151	292	153	5	231	517	306	589	2.42e-75	251
MsaG007931	AT5G38240.1	43.151	292	153	5	231	517	324	607	4.94e-75	251
MsaG007931	AT2G19130.1	48.123	293	143	4	232	518	483	772	4.71e-74	253
MsaG007931	AT5G38280.1	41.214	313	168	7	231	530	320	629	9.17e-74	248

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Find 135 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCGGGAAATATTCTCT+TGG	0.519512	2:+24884157	MsaT007931.1:CDS
AAAAGACATCAAGGAAATGG+TGG	0.596195	2:+24883645	MsaT007931.1:CDS
AAAATAGTGAAAGCCAAGAG+TGG	0.624535	2:+24884393	MsaT007931.1:CDS
AAAATTTGATGCTGGACTAT+TGG	0.279573	2:+24884436	MsaT007931.1:CDS
AAACCAAAATCAGCGACTTT+AGG	0.352958	2:-24884194	None:intergenic
AAAGAGTTACACAATGTCAG+TGG	0.633979	2:-24880021	None:intergenic
AAATGCGATGTTTATAGTTT+TGG	0.248824	2:+24884323	MsaT007931.1:CDS
AAATTGTGAATCTGGACTAA+TGG	0.375108	2:-24883670	None:intergenic
AACTAGTGTTTCTTCTGTTA+TGG	0.310575	2:+24884700	MsaT007931.1:CDS
AAGCTTCATGAGATTGCAAT+TGG	0.427255	2:+24884071	MsaT007931.1:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	24878962	24884799	24878962	ID=MsaG007931
Chr2	mRNA	24878962	24884799	24878962	ID=MsaT007931.1;Parent=MsaG007931
Chr2	exon	24878962	24879071	24878962	ID=MsaT007931.1.exon1;Parent=MsaT007931.1
Chr2	CDS	24878962	24879071	24878962	ID=cds.MsaT007931.1;Parent=MsaT007931.1
Chr2	exon	24879732	24880173	24879732	ID=MsaT007931.1.exon2;Parent=MsaT007931.1
Chr2	CDS	24879732	24880173	24879732	ID=cds.MsaT007931.1;Parent=MsaT007931.1
Chr2	exon	24881865	24881899	24881865	ID=MsaT007931.1.exon3;Parent=MsaT007931.1
Chr2	CDS	24881865	24881899	24881865	ID=cds.MsaT007931.1;Parent=MsaT007931.1
Chr2	exon	24883644	24884799	24883644	ID=MsaT007931.1.exon4;Parent=MsaT007931.1
Chr2	CDS	24883644	24884799	24883644	ID=cds.MsaT007931.1;Parent=MsaT007931.1

Gene Sequence

>MsaT007931.1

ATGAGGAAATATCAAGGGATGAGAGACCATGCAGAACCAGGAAAGATAACTAGGATCAACTTGCCAGTTGGTCTCCACATATGGACCATGCATTTCTTCATGTACAGCATCCGTATTAATTCTGCAAAATCTCAATTCAAACCTGGTAACCATACCAAAAATCCCACTCATCAATCTTCACAACAGCACAAAGACACATTCAATGGTTTGGCCTCCAAGCATGTGACAACTGAGATAGACTCCCTTCAATCTAAAAATAAGGTTGTTGCTCCTCCTTTAGGCACACCATCACAGGTCTCAGTTAGGGCCTCTGAGACAACCTTGTTGCACCATGATCAAGGTGATACGACAAAGTTTCCAAACTCCTTGGATGCTGAACTGACTGAGGGGGAAGAAGATCCACTGACATTGTGTAACTCTTTTGATCTACTCATTACTATGGGGGTTGTCCAAACTGGGAAGGCCCTTTTACCAGACCTGAATATCAATGATTCCAATGCAGGGCAGTTGGCTGCTACGCAATTGGAAGTTTTGGATACTCCTATAGAAACTGTTATAGTGGTCTGGAAGCTTATGAAAGATGGGAAAAAAAGACATCAAGGAAATGGTGGAACCATTAGTCCAGATTCACAATTTATAACACTCACTATGGATAAATTTTTAAACGACATGGAGAGGGAGAAGCCAATAAGATTCACCGGTCAACAACTGAGAATTGCAACAGACAACTACTCCAACTTGCTAGGGTCAGGTGGATTTGGTACAGTTTATAAAGGAATTTTCAGTAACGGAACCATGGTAGCTGTGAAGGTTCTACGTGGTAGTTCTAACAAAAAAATTGACGAACAATTTATGGCAGAAGTTGGAACAATCGGTAGAATTCATCACTTTAATCTCGTTAGGTTATATGGATTTTGCTTTGAAAGAAACTTGATAGCGCTTGTTTATGAATACATGGGAAATGGATCACTTGATAGGTATTTGTTTCATGAAACAAAAGTGTTAGGATATGAAAAGCTTCATGAGATTGCAATTGGTACGGCAAGAGGCATTGCTTACTTGCACGAAGAGTGCCAACATAGAATAATCCACTACGATATAAAACCGGGAAATATTCTCTTGGATAAGAACTTCTATCCTAAAGTCGCTGATTTTGGTTTGGCAAAAAATTGCAACAGGGAAAATACTCACATAACCATGACCGGAGGAAGGGGGACGCCTGGTTACGCCGCACCTGAGCTTTGGATGCCATTTCCTATAACTCACAAATGCGATGTTTATAGTTTTGGTATGCTATTATTTGAAATTATAGGTAGGAGAAGAAACCTTGACATTAAAAATAGTGAAAGCCAAGAGTGGTTTCCAATTTGGGTTTGGAAAAAATTTGATGCTGGACTATTGGAGGAGGCAATGATAGTTTGTGGAATAGAAGAGAAAAATAGAGAGATTGCTGAAAGAATGGTTAAGGTAGCTTTGTGGTGTGTTCAATATAGGCAAGAATTAAGGCCTATAATGAGTGTTGTGGTGAAAATGTTGGAAGGTTCATTAGAAATTCCAAAGACTTTTAACCCATTTCAACATTTGATTGATGGGACTAAGTTTACTACTCATTCAGAACAAGAGTCAAACTCAAATGCATACACAACTAGTGTTTCTTCTGTTATGGTAAGTGATTCCAGCATTGTATGTGCTACTCCTATTATGAGGAAATATGAGATTGAATTGGCATCTAGCACAGCTTGA

Protein sequence

>MsaT007931.1

MRKYQGMRDHAEPGKITRINLPVGLHIWTMHFFMYSIRINSAKSQFKPGNHTKNPTHQSSQQHKDTFNGLASKHVTTEIDSLQSKNKVVAPPLGTPSQVSVRASETTLLHHDQGDTTKFPNSLDAELTEGEEDPLTLCNSFDLLITMGVVQTGKALLPDLNINDSNAGQLAATQLEVLDTPIETVIVVWKLMKDGKKRHQGNGGTISPDSQFITLTMDKFLNDMEREKPIRFTGQQLRIATDNYSNLLGSGGFGTVYKGIFSNGTMVAVKVLRGSSNKKIDEQFMAEVGTIGRIHHFNLVRLYGFCFERNLIALVYEYMGNGSLDRYLFHETKVLGYEKLHEIAIGTARGIAYLHEECQHRIIHYDIKPGNILLDKNFYPKVADFGLAKNCNRENTHITMTGGRGTPGYAAPELWMPFPITHKCDVYSFGMLLFEIIGRRRNLDIKNSESQEWFPIWVWKKFDAGLLEEAMIVCGIEEKNREIAERMVKVALWCVQYRQELRPIMSVVVKMLEGSLEIPKTFNPFQHLIDGTKFTTHSEQESNSNAYTTSVSSVMVSDSSIVCATPIMRKYEIELASSTA*