AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008004


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008004 MtrunA17_Chr2g0298701 93.305 717 32 2 1 711 1 707 0.0 1304
MsaG008004 MtrunA17_Chr1g0207661 47.210 233 115 3 139 371 245 469 4.54e-58 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008004 AT4G29940.1 58.118 425 158 5 136 546 97 515 1.81e-155 470
MsaG008004 AT4G29940.2 58.118 425 158 5 136 546 97 515 1.90e-155 469
MsaG008004 AT3G19510.1 46.586 249 121 3 121 369 160 396 7.36e-61 218
MsaG008004 AT3G19510.2 46.586 249 121 3 121 369 160 396 7.36e-61 218

Find 127 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAAGTGTGGAGGTATTTC+TGG 0.146341 2:-26404632 None:intergenic
AGGGCGTCCTTCACGGATTT+TGG 0.158407 2:-26403961 None:intergenic
AAGAACAATGATAGAATAAA+AGG 0.187496 2:+26403723 MsaT008004.1:CDS
TCCAGCATGTGGTCTTTAAA+TGG 0.227946 2:+26402953 MsaT008004.1:CDS
ATCCTTGGTTGGCACCTTAT+TGG 0.235926 2:-26401205 None:intergenic
CAAAACCAGCCTTGGTCTTC+AGG 0.247836 2:-26402211 None:intergenic
CCTCCACACTTGGGAGAAAA+TGG 0.255233 2:+26404641 MsaT008004.1:CDS
CTATCATTGTTCTTATTATT+CGG 0.255964 2:-26403714 None:intergenic
TCAGTCTGTGTTTCTTCCTT+TGG 0.278404 2:-26401119 None:intergenic
AACCTGCCAAGTACTGTCTA+AGG 0.296673 2:-26402299 None:intergenic
TGGCTTTCTTCCTTCCTCTC+TGG 0.343007 2:-26402872 None:intergenic
AGATATTCCTTGTTCTTCTC+TGG 0.348606 2:-26401437 None:intergenic
AGTTACTGATTCTTCCAATA+AGG 0.353196 2:+26401191 MsaT008004.1:CDS
TGACTGTAAGATAGAAATAT+TGG 0.355208 2:+26402243 MsaT008004.1:CDS
GGATTATAGTCATCGTCTTC+AGG 0.355847 2:-26402851 None:intergenic
TTTCGAAAGGGCGTCCTTCA+CGG 0.358216 2:-26403968 None:intergenic
AGAATAAAAGGGAATTCTTC+AGG 0.374854 2:+26403735 MsaT008004.1:CDS
TCAAATAAAAGATTGATTGT+TGG 0.379038 2:+26401231 MsaT008004.1:CDS
CAGAGTATAATATCATTATC+TGG 0.390452 2:-26402021 None:intergenic
GAAGATGAAGATTGGGGTCC+TGG 0.398942 2:+26403615 MsaT008004.1:CDS
AGATTGAAGCAAAGATTAAC+TGG 0.399258 2:+26405526 MsaT008004.1:CDS
GGACGCCCTTTCGAAAGAAA+TGG 0.401279 2:+26403975 MsaT008004.1:CDS
AAGAAGTGTGAAAATGTTAC+TGG 0.405838 2:+26404563 MsaT008004.1:CDS
ATGTTCCTATAGCTGAGTTA+AGG 0.407231 2:+26405611 MsaT008004.1:CDS
AAACTTCTCTTAATCATGAT+AGG 0.417638 2:+26401304 MsaT008004.1:CDS
AGTTCAGTTGGTAGTATTGA+AGG 0.418881 2:+26401823 MsaT008004.1:CDS
CACATAGATTCTGATGAATC+CGG 0.421682 2:+26403028 MsaT008004.1:CDS
CCGTGATGCCATTCACCAAC+TGG 0.421979 2:+26401792 MsaT008004.1:CDS
GAGATGTCTTGATCCTCCTT+TGG 0.422829 2:+26402074 MsaT008004.1:CDS
AGTGAGTGAAGATGAAGATT+GGG 0.435286 2:+26403608 MsaT008004.1:CDS
CCCCTTAGACAGTACTTGGC+AGG 0.439188 2:+26402297 MsaT008004.1:CDS
ATAGGAACATACATAATAGA+TGG 0.443496 2:-26405598 None:intergenic
CGATAATGCAGGTTAACAAA+TGG 0.446754 2:+26404176 MsaT008004.1:intron
ACATGTTCCTTTGTGTTACT+CGG 0.451425 2:-26401048 None:intergenic
TGTTCCTATAGCTGAGTTAA+GGG 0.455270 2:+26405612 MsaT008004.1:CDS
TAATGAAACTATTGCAAGAA+AGG 0.457497 2:+26405413 MsaT008004.1:CDS
AGACTCCGAGGCAGCAGAGT+TGG 0.457827 2:+26405465 MsaT008004.1:CDS
AAGTGAGTGAAGATGAAGAT+TGG 0.458660 2:+26403607 MsaT008004.1:CDS
AGGCAACAAACGCGCATCTT+GGG 0.460252 2:+26402266 MsaT008004.1:CDS
GTTCAAAATTGCAGATCAAA+GGG 0.463923 2:+26405550 MsaT008004.1:CDS
GAGGCAACAAACGCGCATCT+TGG 0.466032 2:+26402265 MsaT008004.1:CDS
AAATTCCAACTCTGCTGCCT+CGG 0.470562 2:-26405470 None:intergenic
CTGGATTCTCTTAGTTCAGT+TGG 0.473153 2:+26401811 MsaT008004.1:CDS
ATTTCCCCTTAGACAGTACT+TGG 0.477866 2:+26402293 MsaT008004.1:CDS
AAAGTCTACATAAAACTGAT+AGG 0.478611 2:+26401256 MsaT008004.1:CDS
TTCCTCCTGAAGACCAAGGC+TGG 0.484945 2:+26402206 MsaT008004.1:CDS
CAAGTCCCATTTCTTTCGAA+AGG 0.486339 2:-26403981 None:intergenic
TATAGCTGAGTTAAGGGAAA+AGG 0.488966 2:+26405618 MsaT008004.1:CDS
AGAACAATGATAGAATAAAA+GGG 0.492512 2:+26403724 MsaT008004.1:CDS
GACGCCCTTTCGAAAGAAAT+GGG 0.493793 2:+26403976 MsaT008004.1:CDS
AGTAATGCAGCATCGCCGTC+AGG 0.494282 2:-26402797 None:intergenic
ACCAGAAATACCTCCACACT+TGG 0.495132 2:+26404631 MsaT008004.1:CDS
AAGTCCCATTTCTTTCGAAA+GGG 0.495232 2:-26403980 None:intergenic
CTTCTGTGTTGATGCCACCA+TGG 0.498525 2:-26402892 None:intergenic
TACTAAATCAAGAAGAAGAA+TGG 0.501022 2:+26402816 MsaT008004.1:CDS
AGTTCAAAATTGCAGATCAA+AGG 0.506042 2:+26405549 MsaT008004.1:CDS
CTCCAGCGTAAGCATCGATA+AGG 0.509397 2:-26401485 None:intergenic
TTATTTGAAGAATCCTTGGT+TGG 0.511651 2:-26401216 None:intergenic
ACGACCACATGCTATTTCCC+CGG 0.515781 2:-26403047 None:intergenic
ATCGATGCTTACGCTGGAGA+AGG 0.517287 2:+26401489 MsaT008004.1:CDS
GTCACAGTCGAGAGAAGATT+AGG 0.517689 2:+26401713 MsaT008004.1:intron
TTCTACATCACAACATGTGC+AGG 0.518102 2:+26404487 MsaT008004.1:CDS
CATGGCACTTAAAATGAGAA+AGG 0.518506 2:+26404216 MsaT008004.1:CDS
TCCATTTAAAGACCACATGC+TGG 0.518896 2:-26402954 None:intergenic
AAGAGAATCCAGTTGGTGAA+TGG 0.519329 2:-26401800 None:intergenic
GGTCGTTAGAATAATATTCA+AGG 0.522036 2:-26403006 None:intergenic
AGAGAAGATTAGGCCAGAAA+AGG 0.525646 2:+26401723 MsaT008004.1:CDS
AAGGAGGATCAAGACATCTC+TGG 0.528780 2:-26402071 None:intergenic
ACATAGATTCTGATGAATCC+GGG 0.537290 2:+26403029 MsaT008004.1:CDS
AAAGGAACATGTCAAGTTGA+AGG 0.541741 2:+26401059 MsaT008004.1:CDS
GGTTCTGTCATTGCTACAGA+TGG 0.542741 2:+26401844 MsaT008004.1:CDS
ACCTGCCAAGTACTGTCTAA+GGG 0.543897 2:-26402298 None:intergenic
AACCTTATCGATGCTTACGC+TGG 0.549016 2:+26401483 MsaT008004.1:CDS
AATACTTGCAAAACCAGCCT+TGG 0.554312 2:-26402219 None:intergenic
ATGCTTACGCTGGAGAAGGT+TGG 0.555448 2:+26401493 MsaT008004.1:CDS
TATTCCTTGTTCTTCTCTGG+AGG 0.556906 2:-26401434 None:intergenic
GAATGTTCTATGTTAGATGA+AGG 0.561374 2:+26402977 MsaT008004.1:CDS
GAGAAGAGAAAAGGAGTCTG+AGG 0.568921 2:+26403647 MsaT008004.1:CDS
TCTTCACTCACTTGTTCACA+AGG 0.570005 2:-26403597 None:intergenic
AACATCATCACTGACCTCCA+TGG 0.580335 2:-26405386 None:intergenic
TGACTATAATCCAGAGAGGA+AGG 0.581091 2:+26402862 MsaT008004.1:CDS
GGGAAATAGCATGTGGTCGT+AGG 0.581992 2:+26403050 MsaT008004.1:CDS
AGGAAAGGTGAGATAGATGT+TGG 0.583618 2:+26401324 MsaT008004.1:CDS
ACAACATGTGCAGGAAGAAG+AGG 0.585587 2:+26404496 MsaT008004.1:CDS
AATGGGACTTGATGCTGCAA+AGG 0.589378 2:+26403993 MsaT008004.1:CDS
AATGATATTATACTCTGTGA+TGG 0.590683 2:+26402027 MsaT008004.1:CDS
GATAGATGTTGGTGATCAAG+AGG 0.591239 2:+26401335 MsaT008004.1:CDS
TTTGTTTAGCTTCAAGAAGG+AGG 0.591319 2:+26404425 MsaT008004.1:intron
CAAGTAGCACAAAACACCAA+AGG 0.591492 2:+26401103 MsaT008004.1:CDS
TCTCTTAATCATGATAGGAA+AGG 0.591668 2:+26401309 MsaT008004.1:CDS
CAAGTAGTTCTTCCAGCATG+TGG 0.596343 2:+26402942 MsaT008004.1:CDS
TTCCAATAAGGTGCCAACCA+AGG 0.598203 2:+26401203 MsaT008004.1:CDS
CTGTAAGATAGAAATATTGG+AGG 0.603472 2:+26402246 MsaT008004.1:CDS
CCAGTTGGTGAATGGCATCA+CGG 0.605629 2:-26401792 None:intergenic
AAATCAAAATCTCATAAACT+CGG 0.606698 2:+26401141 MsaT008004.1:CDS
TACGCTGGAGAAGGTTGGAA+AGG 0.611602 2:+26401498 MsaT008004.1:CDS
CATGGTGGCATCAACACAGA+AGG 0.612139 2:+26402893 MsaT008004.1:CDS
GAAGAAGCTGCTATTCCTGA+CGG 0.614571 2:+26402782 MsaT008004.1:CDS
TATCACTTCTAAGAACTCAA+AGG 0.617285 2:+26404463 MsaT008004.1:CDS
CGGAGGTTGCACAAATGTGT+CGG 0.617900 2:+26401161 MsaT008004.1:CDS
CTGAACTAAGAGAATCCAGT+TGG 0.618760 2:-26401807 None:intergenic
CTGTCTTCAGTCTCCAAAGG+AGG 0.618854 2:-26402087 None:intergenic
TCACTGTCTTCAGTCTCCAA+AGG 0.619248 2:-26402090 None:intergenic
CGGATTCATCAGAATCTATG+TGG 0.622262 2:-26403027 None:intergenic
CCTGCCAAGTACTGTCTAAG+GGG 0.632462 2:-26402297 None:intergenic
GAGAGGAAGGAAGAAAGCCA+TGG 0.637015 2:+26402875 MsaT008004.1:CDS
ACTGAGAAGTTAAATAACCA+AGG 0.637280 2:+26401015 MsaT008004.1:CDS
CTTGGGAGAAAATGGCAACA+AGG 0.637413 2:+26404649 MsaT008004.1:CDS
TCATGTGAAAACCTAAAACA+AGG 0.637563 2:-26403768 None:intergenic
TCGTCTTCAGGATCATCTGA+CGG 0.640667 2:-26402839 None:intergenic
CCAGAAATACCTCCACACTT+GGG 0.646657 2:+26404632 MsaT008004.1:CDS
GAGAAGGTTGGAAAGGTCAG+AGG 0.649501 2:+26401505 MsaT008004.1:CDS
AGGAAGGAAGAAAGCCATGG+TGG 0.650653 2:+26402878 MsaT008004.1:CDS
GTGAGTGAAGATGAAGATTG+GGG 0.652036 2:+26403609 MsaT008004.1:CDS
AACCAGCCTTGGTCTTCAGG+AGG 0.652976 2:-26402208 None:intergenic
CATAGATTCTGATGAATCCG+GGG 0.669012 2:+26403030 MsaT008004.1:CDS
TACAAAACCGAGTAACACAA+AGG 0.669844 2:+26401041 MsaT008004.1:CDS
GCTTCCTCCAAAATCCGTGA+AGG 0.670380 2:+26403954 MsaT008004.1:CDS
GCAGTTCCTCCTGAAGACCA+AGG 0.671156 2:+26402202 MsaT008004.1:intron
TCACTCACTTGTTCACAAGG+TGG 0.675741 2:-26403594 None:intergenic
TGCATTTGAAGGAGACTCCG+AGG 0.681549 2:+26405453 MsaT008004.1:CDS
TCAAAATCTCATAAACTCGG+AGG 0.684427 2:+26401144 MsaT008004.1:CDS
TTATCTTCAGGATTCCATGG+AGG 0.700522 2:+26405372 MsaT008004.1:intron
GAATCCGGGGAAATAGCATG+TGG 0.711313 2:+26403043 MsaT008004.1:CDS
ACGATGACTATAATCCAGAG+AGG 0.733914 2:+26402858 MsaT008004.1:CDS
TACGCCTCCAGAGAAGAACA+AGG 0.736747 2:+26401430 MsaT008004.1:CDS
AATTCAACAAAGATACAACG+AGG 0.805911 2:+26401279 MsaT008004.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 26401006 26405648 26401006 ID=MsaG008004
Chr2 mRNA 26401006 26405648 26401006 ID=MsaT008004.1;Parent=MsaG008004
Chr2 exon 26401006 26401526 26401006 ID=MsaT008004.1.exon1;Parent=MsaT008004.1
Chr2 CDS 26401006 26401526 26401006 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26401720 26401885 26401720 ID=MsaT008004.1.exon2;Parent=MsaT008004.1
Chr2 CDS 26401720 26401885 26401720 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26401985 26402108 26401985 ID=MsaT008004.1.exon3;Parent=MsaT008004.1
Chr2 CDS 26401985 26402108 26401985 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26402206 26402318 26402206 ID=MsaT008004.1.exon4;Parent=MsaT008004.1
Chr2 CDS 26402206 26402318 26402206 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26402770 26403108 26402770 ID=MsaT008004.1.exon5;Parent=MsaT008004.1
Chr2 CDS 26402770 26403108 26402770 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26403573 26403800 26403573 ID=MsaT008004.1.exon6;Parent=MsaT008004.1
Chr2 CDS 26403573 26403800 26403573 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26403925 26404014 26403925 ID=MsaT008004.1.exon7;Parent=MsaT008004.1
Chr2 CDS 26403925 26404014 26403925 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26404187 26404237 26404187 ID=MsaT008004.1.exon8;Parent=MsaT008004.1
Chr2 CDS 26404187 26404237 26404187 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26404434 26404670 26404434 ID=MsaT008004.1.exon9;Parent=MsaT008004.1
Chr2 CDS 26404434 26404670 26404434 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Chr2 exon 26405382 26405648 26405382 ID=MsaT008004.1.exon10;Parent=MsaT008004.1
Chr2 CDS 26405382 26405648 26405382 ID=cds.MsaT008004.1;Parent=MsaT008004.1
Gene Sequence

>MsaT008004.1

ATGCGGGATACTGAGAAGTTAAATAACCAAGGATCTACAAAACCGAGTAACACAAAGGAACATGTCAAGTTGAAGGTAGATTCCCCCCAGTTCAAAACAAGTAGCACAAAACACCAAAGGAAGAAACACAGACTGAAATCAAAATCTCATAAACTCGGAGGTTGCACAAATGTGTCGGTGAAGACAGTTACTGATTCTTCCAATAAGGTGCCAACCAAGGATTCTTCAAATAAAAGATTGATTGTTGGACAAAGTCTACATAAAACTGATAGGAATTCAACAAAGATACAACGAGGAAAAACTTCTCTTAATCATGATAGGAAAGGTGAGATAGATGTTGGTGATCAAGAGGAAAATATTCAAAAACGTAAGAGAAGGAGAAAGAAGAAAAGACAGAGACATGATGTAGATCTTGATGATACTGTACGCCTCCAGAGAAGAACAAGGAATATCTTAATCAGAATGAAGCAAGAGCAGAACCTTATCGATGCTTACGCTGGAGAAGGTTGGAAAGGTCAGAGTCGAGAGAAGATTAGGCCAGAAAAGGAGCTGCAACGAGCAAAAAAGCAGATTTTAAAATGCAAGCTTAGTATCCGTGATGCCATTCACCAACTGGATTCTCTTAGTTCAGTTGGTAGTATTGAAGGTTCTGTCATTGCTACAGATGGATCTGTTTCTCATGAACATATATTCTGTGCAAATTGCAAGATAAACGAAGTTTCTCCAGATAATGATATTATACTCTGTGATGGCACATGCAATCGTGCGTTTCACCAGAGATGTCTTGATCCTCCTTTGGAGACTGAAGACATTCCTCCTGAAGACCAAGGCTGGTTTTGCAAGTATTGTGACTGTAAGATAGAAATATTGGAGGCAACAAACGCGCATCTTGGGACTCATTTCCCCTTAGACAGTACTTGGCAGGATGTATTCAAGGAAGAAGCTGCTATTCCTGACGGCGATGCTGCATTACTAAATCAAGAAGAAGAATGGCCGTCAGATGATCCTGAAGACGATGACTATAATCCAGAGAGGAAGGAAGAAAGCCATGGTGGCATCAACACAGAAGGAAATGATGAAAACGCATCTGATGATTCAAGTAGTTCTTCCAGCATGTGGTCTTTAAATGGAGAATGTTCTATGTTAGATGAAGGCATCAACCTTGAATATTATTCTAACGACCACATAGATTCTGATGAATCCGGGGAAATAGCATGTGGTCGTAGGCAGCGTAGAGCTGTTGACTACAAGAAGCTTTATGATGAAATGTTTGGAAAGGATGCTCCACCTTGTGAACAAGTGAGTGAAGATGAAGATTGGGGTCCTGGAAAAAGACGGAGAAGAGAAAAGGAGTCTGAGGCAGTTAATACTCTTATGACTTTACATGAGAGTGAAAATAAATATCCGAATAATAAGAACAATGATAGAATAAAAGGGAATTCTTCAGGCATAAAAAGACCTTGTTTTAGGTTTTCACATGATGCAGTTGAGAAGCTTCGCCAAGTTTTTGCAGAGAATGAGCTTCCTCCAAAATCCGTGAAGGACGCCCTTTCGAAAGAAATGGGACTTGATGCTGCAAAGGTTAACAAATGGTTCAAAAATGCACGTTACATGGCACTTAAAATGAGAAAGCTTCAAGAAGGAGGACAACAGCTTCAGAGTATCACTTCTAAGAACTCAAAGGATTCTACATCACAACATGTGCAGGAAGAAGAGGTTTTGAACCCGAAGATCGCAAAAATTACTGTGGTTCGTCCCTTGAAGAAGTGTGAAAATGTTACTGGCAAAGAAAAAACAAAAGCATCTAGCAGTCCTTTGAAGAAAAAGCGACCAGAAATACCTCCACACTTGGGAGAAAATGGCAACAAGGATTCCATGGAGGTCAGTGATGATGTTAGCTTAATGAAACTATTGCAAGAAAGGAAAAAGAAGGTAAGTTTTGCATTTGAAGGAGACTCCGAGGCAGCAGAGTTGGAATTTGAAAGACTCAGCAAAGTGAAGACGAAAATAGATAGATTGAAGCAAAGATTAACTGGAGTTCAAAATTGCAGATCAAAGGGTTCAGATGAAGTTCATTTGAATGAGCCATCTATTATGTATGTTCCTATAGCTGAGTTAAGGGAAAAGGTGAAATAG

Protein sequence

>MsaT008004.1

MRDTEKLNNQGSTKPSNTKEHVKLKVDSPQFKTSSTKHQRKKHRLKSKSHKLGGCTNVSVKTVTDSSNKVPTKDSSNKRLIVGQSLHKTDRNSTKIQRGKTSLNHDRKGEIDVGDQEENIQKRKRRRKKKRQRHDVDLDDTVRLQRRTRNILIRMKQEQNLIDAYAGEGWKGQSREKIRPEKELQRAKKQILKCKLSIRDAIHQLDSLSSVGSIEGSVIATDGSVSHEHIFCANCKINEVSPDNDIILCDGTCNRAFHQRCLDPPLETEDIPPEDQGWFCKYCDCKIEILEATNAHLGTHFPLDSTWQDVFKEEAAIPDGDAALLNQEEEWPSDDPEDDDYNPERKEESHGGINTEGNDENASDDSSSSSSMWSLNGECSMLDEGINLEYYSNDHIDSDESGEIACGRRQRRAVDYKKLYDEMFGKDAPPCEQVSEDEDWGPGKRRRREKESEAVNTLMTLHESENKYPNNKNNDRIKGNSSGIKRPCFRFSHDAVEKLRQVFAENELPPKSVKDALSKEMGLDAAKVNKWFKNARYMALKMRKLQEGGQQLQSITSKNSKDSTSQHVQEEEVLNPKIAKITVVRPLKKCENVTGKEKTKASSSPLKKKRPEIPPHLGENGNKDSMEVSDDVSLMKLLQERKKKVSFAFEGDSEAAELEFERLSKVKTKIDRLKQRLTGVQNCRSKGSDEVHLNEPSIMYVPIAELREKVK*