AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008123

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008123 MtrunA17_Chr2g0300241 99.605 253 1 0 1 253 1 253 0.0 521
MsaG008123 MtrunA17_Chr8g0343441 74.016 254 56 5 8 253 5 256 5.83e-117 335
MsaG008123 MtrunA17_Chr1g0150821 73.282 262 52 3 7 253 5 263 3.79e-114 328
MsaG008123 MtrunA17_Chr2g0329821 72.177 248 58 5 13 252 6 250 1.09e-106 308
MsaG008123 MtrunA17_Chr4g0076771 68.548 248 68 6 12 252 10 254 1.55e-105 306
MsaG008123 MtrunA17_Chr4g0006381 59.231 260 81 6 1 253 1 242 2.35e-89 264
MsaG008123 MtrunA17_Chr7g0242511 57.647 255 93 6 1 253 1 242 2.54e-84 251
MsaG008123 MtrunA17_Chr8g0366371 90.000 80 8 0 133 212 2 81 1.30e-24 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008123 AT5G20510.1 68.702 262 69 3 3 253 1 260 5.33e-119 340
MsaG008123 AT3G42790.1 68.775 253 72 4 3 252 1 249 2.97e-110 318
MsaG008123 AT2G02470.1 69.444 252 63 6 12 253 9 256 5.37e-107 310
MsaG008123 AT2G02470.2 69.758 248 60 6 12 253 9 247 6.11e-105 304
MsaG008123 AT1G14510.1 70.082 244 64 5 12 250 9 248 1.31e-101 296
MsaG008123 AT5G26210.1 71.542 253 63 2 7 252 4 254 3.23e-100 293
MsaG008123 AT5G20510.2 67.890 218 57 3 47 253 4 219 5.65e-92 270
MsaG008123 AT5G05610.2 62.140 243 82 6 11 252 7 240 2.34e-88 262
MsaG008123 AT5G05610.1 62.140 243 82 6 11 252 7 240 2.34e-88 262
MsaG008123 AT3G11200.1 61.508 252 88 5 3 252 1 245 4.41e-86 256
MsaG008123 AT5G20510.3 75.758 165 40 0 3 167 1 165 5.76e-84 249
MsaG008123 AT3G11200.2 61.111 216 75 5 39 252 24 232 6.79e-71 217
MsaG008123 AT2G02470.3 75.796 157 36 2 12 168 9 163 1.38e-69 215

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATATCCCGGTGAATTA+TGG 0.229806 2:-28730690 MsaT008123.1:CDS
CCGGACTCTCTTATTGTTGC+AGG 0.298666 2:+28728875 None:intergenic
TTCTACTTCGGCGCAAGATT+TGG 0.307978 2:-28730523 MsaT008123.1:CDS
TTGATGCCCAATGCAGGTTC+TGG 0.338942 2:+28730628 None:intergenic
TATCTGCATCAGATGAGTTC+TGG 0.365648 2:-28728992 MsaT008123.1:CDS
TGGATATCCCGGTGAATTAT+GGG 0.422923 2:-28730689 MsaT008123.1:CDS
AAGGAGAATCTTTCTCTGTA+TGG 0.424220 2:-28730709 MsaT008123.1:CDS
GACTTCAAGGGCCGTAGAGC+TGG 0.434123 2:-28732289 MsaT008123.1:CDS
AGCTGTCTTCCGTGACTTCA+AGG 0.434860 2:-28732302 MsaT008123.1:CDS
TGATCTGCCAACAATATTTG+AGG 0.435492 2:-28729470 MsaT008123.1:CDS
CTCTCTGTGGCTTTCTACTT+CGG 0.439785 2:-28730535 MsaT008123.1:CDS
AAAAGGGTCATGGAACTTGC+TGG 0.449501 2:-28732358 None:intergenic
CATGCAGCGTGGATCTGAAC+TGG 0.451670 2:-28729111 MsaT008123.1:intron
AATGCAGGTTCTGGAAGTTC+AGG 0.475540 2:+28730637 None:intergenic
TTGATTAAAGCTCTCACCAC+TGG 0.479655 2:-28732265 MsaT008123.1:intron
CTGTTGTGACTTGAGACAGA+TGG 0.481520 2:+28729409 None:intergenic
TTGATGTGCTCAGCCCGGGC+AGG 0.484353 2:+28728915 None:intergenic
TGAACTTCCAGAACCTGCAT+TGG 0.488308 2:-28730635 MsaT008123.1:CDS
CTGCTTGATGTGCTCAGCCC+GGG 0.490873 2:+28728911 None:intergenic
ACAATATTTGAGGTTGTGAC+CGG 0.491460 2:-28729460 MsaT008123.1:CDS
TTGCTGTTCACAGTGATACA+TGG 0.492333 2:-28730561 MsaT008123.1:CDS
ACTGCTTGATGTGCTCAGCC+CGG 0.493704 2:+28728910 None:intergenic
GAACTTCCAGAACCTGCATT+GGG 0.499838 2:-28730634 MsaT008123.1:CDS
TCAGGAGGAACTTCTTCAGC+AGG 0.502111 2:+28730655 None:intergenic
GTGTGAAGATCACTCCTGCC+CGG 0.508233 2:-28728929 MsaT008123.1:CDS
ATCTGCTAAGAAGCAGACTA+AGG 0.509595 2:-28729437 MsaT008123.1:CDS
AGTCTGCTTCTTAGCAGATC+CGG 0.514682 2:+28729441 None:intergenic
TTCCCTATGGTATTCAAGGC+CGG 0.515731 2:+28728856 None:intergenic
AACAGCAACAAGTCAAAGTC+AGG 0.516207 2:-28729391 MsaT008123.1:CDS
GCAAAATTGATGCCCAATGC+AGG 0.517201 2:+28730622 None:intergenic
ATATGCGAGAAGTGGTATCA+TGG 0.517493 2:-28728957 MsaT008123.1:CDS
CTTTCTCTGTATGGATATCC+CGG 0.520026 2:-28730700 MsaT008123.1:CDS
CCTAACCAGGGTCACACTGC+TGG 0.524327 2:+28732125 None:intergenic
GACGACGAAGAGGAGGATCA+AGG 0.529279 2:-28729044 MsaT008123.1:CDS
CTCTACGGCCCTTGAAGTCA+CGG 0.531441 2:+28732293 None:intergenic
ATGGGATGCAAGAAAAGGAT+TGG 0.537031 2:-28730594 MsaT008123.1:CDS
GTCATGGAACTTGCTGGTGA+TGG 0.537299 2:-28732352 MsaT008123.1:CDS
GATCATTAATCAGAGTGAAA+AGG 0.538004 2:+28729488 None:intergenic
GTCACAACCTCAAATATTGT+TGG 0.541746 2:+28729463 None:intergenic
ACGACGAAGAGGAGGATCAA+GGG 0.543159 2:-28729043 MsaT008123.1:CDS
TCAACAGTACGAGGGTCAAA+CGG 0.546490 2:+28732325 None:intergenic
AAAAGAAGATGACGAGGGAG+TGG 0.555346 2:-28729066 MsaT008123.1:CDS
TAGGGATGGGATGCAAGAAA+AGG 0.555698 2:-28730599 MsaT008123.1:CDS
AGTGACTTCCCATAATTCAC+CGG 0.557164 2:+28730681 None:intergenic
TGTGAAGATCACTCCTGCCC+GGG 0.562262 2:-28728928 MsaT008123.1:CDS
CCTGCAACAATAAGAGAGTC+CGG 0.564359 2:-28728875 MsaT008123.1:CDS
GCTGTCTTCCGTGACTTCAA+GGG 0.569487 2:-28732301 MsaT008123.1:CDS
ACTCTCTTATTGTTGCAGGC+AGG 0.572502 2:+28728879 None:intergenic
CTTTAATCAAGCCAGCTCTA+CGG 0.578337 2:+28732278 None:intergenic
TTGGCTTTGATAAAGCCGAC+AGG 0.579784 2:-28730504 MsaT008123.1:intron
GCCAGCAAAAGAAGATGACG+AGG 0.593624 2:-28729072 MsaT008123.1:CDS
CAAGGGGAATGCGCTGCATG+CGG 0.601102 2:-28729026 MsaT008123.1:CDS
TGATACATGGCTTCTCTCTG+TGG 0.605581 2:-28730548 MsaT008123.1:CDS
CGACGAAGAGGAGGATCAAG+GGG 0.610579 2:-28729042 MsaT008123.1:CDS
GCAGGTTCTGGAAGTTCAGG+AGG 0.623597 2:+28730640 None:intergenic
CGAGGGAGTGGACGACGAAG+AGG 0.626370 2:-28729054 MsaT008123.1:CDS
GGGAGTGGACGACGAAGAGG+AGG 0.630295 2:-28729051 MsaT008123.1:CDS
GTGACTTCCCATAATTCACC+GGG 0.639519 2:+28730682 None:intergenic
GTCAAAGTCAGGCTCAAAAG+CGG 0.660535 2:-28729380 MsaT008123.1:intron
AAGACAGCTTCAACAGTACG+AGG 0.662619 2:+28732316 None:intergenic
GCTGTGACATATGCGAGAAG+TGG 0.668882 2:-28728965 MsaT008123.1:CDS
AGACAGCTTCAACAGTACGA+GGG 0.736429 2:+28732317 None:intergenic
CCAGCAAAAGAAGATGACGA+GGG 0.742179 2:-28729071 MsaT008123.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 28728869 28732371 28728869 ID=MsaG008123
Chr2 mRNA 28728869 28732371 28728869 ID=MsaT008123.1;Parent=MsaG008123
Chr2 exon 28728869 28729126 28728869 ID=MsaT008123.1.exon5;Parent=MsaT008123.1
Chr2 CDS 28728869 28729126 28728869 ID=cds.MsaT008123.1;Parent=MsaT008123.1
Chr2 exon 28729381 28729516 28729381 ID=MsaT008123.1.exon4;Parent=MsaT008123.1
Chr2 CDS 28729381 28729516 28729381 ID=cds.MsaT008123.1;Parent=MsaT008123.1
Chr2 exon 28730505 28730733 28730505 ID=MsaT008123.1.exon3;Parent=MsaT008123.1
Chr2 CDS 28730505 28730733 28730505 ID=cds.MsaT008123.1;Parent=MsaT008123.1
Chr2 exon 28732131 28732163 28732131 ID=MsaT008123.1.exon2;Parent=MsaT008123.1
Chr2 CDS 28732131 28732163 28732131 ID=cds.MsaT008123.1;Parent=MsaT008123.1
Chr2 exon 28732266 28732371 28732266 ID=MsaT008123.1.exon1;Parent=MsaT008123.1
Chr2 CDS 28732266 28732371 28732266 ID=cds.MsaT008123.1;Parent=MsaT008123.1
Gene Sequence

>MsaT008123.1

ATGGAACTTGCTGGTGATGGAACTCCGTTTGACCCTCGTACTGTTGAAGCTGTCTTCCGTGACTTCAAGGGCCGTAGAGCTGGCTTGATTAAAGCTCTCACCACTGATGTTGAAGATTTTTACCAGCAGTGTGACCCTGAAAAGGAGAATCTTTCTCTGTATGGATATCCCGGTGAATTATGGGAAGTCACTTTACCTGCTGAAGAAGTTCCTCCTGAACTTCCAGAACCTGCATTGGGCATCAATTTTGCTAGGGATGGGATGCAAGAAAAGGATTGGCTATCTTTAGTTGCTGTTCACAGTGATACATGGCTTCTCTCTGTGGCTTTCTACTTCGGCGCAAGATTTGGCTTTGATAAAGCCGACAGGAAACGCCTTTTCACTCTGATTAATGATCTGCCAACAATATTTGAGGTTGTGACCGGATCTGCTAAGAAGCAGACTAAGGAAAAGCCATCTGTCTCAAGTCACAACAGCAACAAGTCAAAGTCAGGCTCAAAAGCGCGTGGATCTGAACTGGCAAAGTATTCAAAGCCGCCAGCAAAAGAAGATGACGAGGGAGTGGACGACGAAGAGGAGGATCAAGGGGAATGCGCTGCATGCGGTGAAAGCTATGTATCTGCATCAGATGAGTTCTGGATCTGCTGTGACATATGCGAGAAGTGGTATCATGGAAAATGTGTGAAGATCACTCCTGCCCGGGCTGAGCACATCAAGCAGTACAAGTGTCCTGCCTGCAACAATAAGAGAGTCCGGCCTTGA
Protein sequence

>MsaT008123.1

MELAGDGTPFDPRTVEAVFRDFKGRRAGLIKALTTDVEDFYQQCDPEKENLSLYGYPGELWEVTLPAEEVPPELPEPALGINFARDGMQEKDWLSLVAVHSDTWLLSVAFYFGARFGFDKADRKRLFTLINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEGVDDEEEDQGECAACGESYVSASDEFWICCDICEKWYHGKCVKITPARAEHIKQYKCPACNNKRVRP*