AlfalfaGEDB | MsaG008129

M. sativa ssp. caerulea / MsaG008129

KEGG: K08064
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02045.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008129	AFK49645.1	97.021	235	5	1	1	233	1	235	4.45e-167	470

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008129	sp\|Q93ZH2\|NFYA3_ARATH	46.739	184	90	4	26	204	129	309	1.45e-39	142

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008129	I3TAV3	97.021	235	5	1	1	233	1	235	2.13e-167	470

TFs/TRs:

Gene ID	Type	Classification
MsaG008129	TF	NF-YA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG005114	MsaG008129	0.832087	2.086191e-55	6.413186e-53
MsaG008129	MsaG042998	0.800563	2.273281e-48	3.112983e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008129	MtrunA17_Chr2g0300261	97.021	235	5	1	1	233	1	235	4.10e-171	470
MsaG008129	MtrunA17_Chr8g0343451	47.917	192	91	6	11	202	103	285	5.18e-45	152
MsaG008129	MtrunA17_Chr2g0329811	51.007	149	61	4	6	145	108	253	1.93e-38	136
MsaG008129	MtrunA17_Chr3g0103391	59.459	111	38	4	23	133	123	226	4.77e-35	127
MsaG008129	MtrunA17_Chr8g0352251	51.515	132	57	3	33	162	114	240	5.99e-35	126
MsaG008129	MtrunA17_Chr2g0292411	53.774	106	46	2	59	162	95	199	4.62e-30	110
MsaG008129	MtrunA17_Chr1g0177091	45.070	142	62	3	5	145	105	231	4.38e-28	108
MsaG008129	MtrunA17_Chr7g0267581	51.667	120	40	5	32	150	134	236	1.39e-27	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008129	AT1G72830.1	46.739	184	90	4	26	204	129	309	1.47e-40	142
MsaG008129	AT1G72830.2	46.486	185	90	5	26	204	129	310	8.43e-39	137
MsaG008129	AT1G17590.3	48.837	172	64	6	32	192	133	291	3.60e-38	135
MsaG008129	AT1G17590.2	48.837	172	64	6	32	192	133	291	3.60e-38	135
MsaG008129	AT1G17590.4	48.837	172	64	6	32	192	133	291	3.60e-38	135
MsaG008129	AT1G17590.1	48.837	172	64	6	32	192	133	291	3.60e-38	135
MsaG008129	AT1G54160.1	68.367	98	29	1	47	142	145	242	6.86e-38	134
MsaG008129	AT3G14020.2	62.626	99	37	0	48	146	141	239	1.18e-34	125
MsaG008129	AT3G14020.1	62.626	99	37	0	48	146	141	239	1.18e-34	125
MsaG008129	AT1G30500.2	56.364	110	44	3	32	141	58	163	2.56e-33	119
MsaG008129	AT1G30500.1	56.364	110	40	3	32	141	58	159	2.94e-33	119
MsaG008129	AT2G34720.1	51.613	124	53	4	19	138	38	158	4.82e-33	119
MsaG008129	AT2G34720.2	52.586	116	50	3	25	138	11	123	1.41e-32	116
MsaG008129	AT2G34720.3	52.586	116	50	3	25	138	11	123	1.41e-32	116
MsaG008129	AT5G12840.4	53.271	107	45	2	33	139	131	232	1.02e-31	117
MsaG008129	AT5G12840.2	53.271	107	45	2	33	139	131	232	1.02e-31	117
MsaG008129	AT5G12840.5	53.271	107	45	2	33	139	132	233	1.13e-31	117
MsaG008129	AT5G12840.3	53.271	107	45	2	33	139	132	233	1.13e-31	117
MsaG008129	AT5G12840.1	53.271	107	45	2	33	139	132	233	1.13e-31	117
MsaG008129	AT3G20910.1	49.587	121	54	4	33	151	126	241	4.72e-30	114
MsaG008129	AT1G72830.3	41.304	184	75	5	26	204	129	284	6.77e-27	105
MsaG008129	AT5G06510.4	50.000	114	44	4	64	174	23	126	1.10e-23	94.0
MsaG008129	AT5G06510.7	50.000	114	44	4	64	174	23	126	1.10e-23	94.0
MsaG008129	AT5G06510.1	50.000	114	44	4	64	174	118	221	1.85e-23	95.5
MsaG008129	AT5G06510.5	50.000	114	44	4	64	174	118	221	1.85e-23	95.5
MsaG008129	AT5G06510.2	50.000	114	44	4	64	174	118	221	1.85e-23	95.5
MsaG008129	AT5G06510.6	50.000	114	44	4	64	174	118	221	1.85e-23	95.5
MsaG008129	AT5G06510.3	50.000	114	44	4	64	174	69	172	1.94e-23	94.4
MsaG008129	AT3G05690.1	38.830	188	86	7	29	204	102	272	1.92e-22	93.6
MsaG008129	AT3G05690.2	48.246	114	38	4	29	137	139	236	4.11e-22	93.2

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCACTTGATCTTGCACAATA+TGG	0.184413	2:-28842885	MsaT008129.1:CDS
TTTGTCACTGGTTCACATTC+TGG	0.240648	2:-28842348	MsaT008129.1:CDS
CCATCATTTGAGGCAACATT+TGG	0.267754	2:+28842934	None:intergenic
CATGCTTTGAATAGAGTTAG+AGG	0.318785	2:-28842423	MsaT008129.1:CDS
TCTAAGCACCAAACAGCTTT+CGG	0.357916	2:-28842385	MsaT008129.1:CDS
CATCATTTGAGGCAACATTT+GGG	0.363234	2:+28842935	None:intergenic
GAGCAATTGAACTCGGTATC+AGG	0.378172	2:+28843248	None:intergenic
CACTTGATCTTGCACAATAT+GGG	0.384645	2:-28842884	MsaT008129.1:CDS
CCAAATGTTGCCTCAAATGA+TGG	0.389646	2:-28842934	MsaT008129.1:CDS
AGCAACGCGCTTCGGTTGTC+CGG	0.405955	2:-28842107	MsaT008129.1:CDS
TTGAATAGAGTTAGAGGATC+TGG	0.407085	2:-28842417	MsaT008129.1:CDS
CCTAAAACTGAAACTGTTGC+CGG	0.424457	2:+28842218	None:intergenic
GGTGCTCCTCCGCCCATGTT+TGG	0.433491	2:+28842165	None:intergenic
CTCTATTCAAAGCATGTTTG+TGG	0.437986	2:+28842430	None:intergenic
ATTAGACTCCGAAAGCTGTT+TGG	0.442566	2:+28842377	None:intergenic
GCTGTTTGGTGCTTAGAAAT+CGG	0.443184	2:+28842391	None:intergenic
TGGCGAGATTCGTGAAGATA+TGG	0.445004	2:+28842450	None:intergenic
TGCAGTGGAGGACTCACATT+TGG	0.450885	2:-28842138	MsaT008129.1:CDS
CGGAACAAAGCAACGCGCTT+CGG	0.452314	2:-28842115	MsaT008129.1:CDS
CAGCCAGAGCACATGTTTCT+CGG	0.463872	2:-28842195	MsaT008129.1:CDS
GATGGAAGATATGTTATCTC+TGG	0.468775	2:+28842269	None:intergenic
CCCCAGCAACAATTATCAAA+AGG	0.507861	2:-28842325	MsaT008129.1:CDS
TCTCGGAAACTCTCCAAACA+TGG	0.515279	2:-28842178	MsaT008129.1:CDS
TTTCCGAGAAACATGTGCTC+TGG	0.517885	2:+28842192	None:intergenic
GGATGATTCAAAGAGAGAAA+AGG	0.526180	2:+28843269	None:intergenic
TCTAATGCAGAATTTGTCAC+TGG	0.556177	2:-28842360	MsaT008129.1:CDS
CAAATGTTGCCTCAAATGAT+GGG	0.557136	2:-28842933	MsaT008129.1:CDS
AGTTGATCATAATCACTCAA+TGG	0.558578	2:-28843222	MsaT008129.1:intron
AACTCTCCAAACATGGGCGG+AGG	0.560530	2:-28842171	MsaT008129.1:CDS
CCGATCGGAACAACTTGTGA+TGG	0.564255	2:+28842251	None:intergenic
GATGCTAATCCCATCATTTG+AGG	0.565976	2:+28842924	None:intergenic
AAACACTTACAACAGCATGC+GGG	0.571388	2:+28843059	None:intergenic
ATCATTTGAGGCAACATTTG+GGG	0.571616	2:+28842936	None:intergenic
AAGTGGTAATGCAACTCTAG+TGG	0.571902	2:+28842902	None:intergenic
AGTGGAGGACTCACATTTGG+CGG	0.581240	2:-28842135	MsaT008129.1:CDS
CCATATTGTGCAAGATCAAG+TGG	0.592935	2:+28842885	None:intergenic
CCATCACAAGTTGTTCCGAT+CGG	0.593385	2:-28842251	MsaT008129.1:CDS
AATACCATGGTATACTGAGA+AGG	0.610675	2:-28842839	MsaT008129.1:CDS
AACACTTACAACAGCATGCG+GGG	0.613676	2:+28843060	None:intergenic
AACTTGTGAGCAATTGAACT+CGG	0.618219	2:+28843241	None:intergenic
CTCGGAAACTCTCCAAACAT+GGG	0.629942	2:-28842177	MsaT008129.1:CDS
AAAACACTTACAACAGCATG+CGG	0.630340	2:+28843058	None:intergenic
GGAGCACCACAATGCAGTGG+AGG	0.632103	2:-28842150	MsaT008129.1:CDS
GTCAATGCAAAACAATACCA+TGG	0.632585	2:-28842852	MsaT008129.1:CDS
AATAGAGTTAGAGGATCTGG+TGG	0.657703	2:-28842414	MsaT008129.1:CDS
GGAAACTCTCCAAACATGGG+CGG	0.669109	2:-28842174	MsaT008129.1:CDS
GGAGGAGCACCACAATGCAG+TGG	0.675924	2:-28842153	MsaT008129.1:CDS
GTCGCCTTCTCAGTATACCA+TGG	0.679853	2:+28842835	None:intergenic
GTGAGTCCTCCACTGCATTG+TGG	0.681382	2:+28842144	None:intergenic
ACAATCACGAGCTAAGCTTG+AGG	0.693616	2:-28842814	MsaT008129.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	28842104	28843297	28842104	ID=MsaG008129
Chr2	mRNA	28842104	28843297	28842104	ID=MsaT008129.1;Parent=MsaG008129
Chr2	exon	28842104	28842472	28842104	ID=MsaT008129.1.exon4;Parent=MsaT008129.1
Chr2	CDS	28842104	28842472	28842104	ID=cds.MsaT008129.1;Parent=MsaT008129.1
Chr2	exon	28842785	28842964	28842785	ID=MsaT008129.1.exon3;Parent=MsaT008129.1
Chr2	CDS	28842785	28842964	28842785	ID=cds.MsaT008129.1;Parent=MsaT008129.1
Chr2	exon	28843069	28843146	28843069	ID=MsaT008129.1.exon2;Parent=MsaT008129.1
Chr2	CDS	28843069	28843146	28843069	ID=cds.MsaT008129.1;Parent=MsaT008129.1
Chr2	exon	28843223	28843297	28843223	ID=MsaT008129.1.exon1;Parent=MsaT008129.1
Chr2	CDS	28843223	28843297	28843223	ID=cds.MsaT008129.1;Parent=MsaT008129.1

Gene Sequence

>MsaT008129.1

ATGAAGCCTTTTCTCTCTTTGAATCATCCTGATACCGAGTTCAATTGCTCACAAGTTGATCATAATCACTCAATGGCTCAGACTCATGCTCATTATCCTTATAGTGATCCAATTTTTGCTGGTTCGTTAGTTGCTTACGCCCCGCATGCTGTTAATCAACCCCAAATGTTGCCTCAAATGATGGGATTAGCATCCACTAGAGTTGCATTACCACTTGATCTTGCACAATATGGGCCCATTTATGTCAATGCAAAACAATACCATGGTATACTGAGAAGGCGACAATCACGAGCTAAGCTTGAGGCACAGAACAAACTCATCAAAAATCGTAAACCATATCTTCACGAATCTCGCCACAAACATGCTTTGAATAGAGTTAGAGGATCTGGTGGCCGATTTCTAAGCACCAAACAGCTTTCGGAGTCTAATGCAGAATTTGTCACTGGTTCACATTCTGGCCCCAGCAACAATTATCAAAAGGAAGATCATTCAGAGATAGAAAGTCACTCAAAAACCAGAGATAACATATCTTCCATCACAAGTTGTTCCGATCGGACATGTTTTTCCGGCAACAGTTTCAGTTTTAGGCAGCCAGAGCACATGTTTCTCGGAAACTCTCCAAACATGGGCGGAGGAGCACCACAATGCAGTGGAGGACTCACATTTGGCGGAACAAAGCAACGCGCTTCGGTTGTCCGGTGA

Protein sequence

>MsaT008129.1

MKPFLSLNHPDTEFNCSQVDHNHSMAQTHAHYPYSDPIFAGSLVAYAPHAVNQPQMLPQMMGLASTRVALPLDLAQYGPIYVNAKQYHGILRRRQSRAKLEAQNKLIKNRKPYLHESRHKHALNRVRGSGGRFLSTKQLSESNAEFVTGSHSGPSNNYQKEDHSEIESHSKTRDNISSITSCSDRTCFSGNSFSFRQPEHMFLGNSPNMGGGAPQCSGGLTFGGTKQRASVVR*