AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008152

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008152 MtrunA17_Chr3g0091281 80.000 70 14 0 1 70 166 235 7.51e-35 119
MsaG008152 MtrunA17_Chr3g0114511 83.077 65 11 0 1 65 185 249 1.48e-33 120
MsaG008152 MtrunA17_Chr3g0091421 78.571 70 15 0 1 70 166 235 1.98e-33 115
MsaG008152 MtrunA17_Chr7g0244521 70.312 64 19 0 1 64 167 230 2.57e-26 100
MsaG008152 MtrunA17_Chr3g0107011 63.235 68 24 1 1 68 169 235 2.75e-26 100
MsaG008152 MtrunA17_Chr3g0107051 56.180 89 33 2 1 83 176 264 6.14e-26 99.4
MsaG008152 MtrunA17_Chr5g0432471 64.615 65 23 0 1 65 167 231 8.01e-26 99.4
MsaG008152 MtrunA17_Chr2g0284031 51.163 86 40 1 1 84 186 271 4.52e-24 94.4
MsaG008152 MtrunA17_Chr5g0432491 54.430 79 34 1 1 77 179 257 4.55e-24 94.4
MsaG008152 MtrunA17_Chr5g0441011 56.944 72 31 0 1 72 169 240 4.06e-23 91.7
MsaG008152 MtrunA17_Chr4g0013131 62.500 64 24 0 1 64 167 230 9.36e-23 90.5
MsaG008152 MtrunA17_Chr2g0284011 59.375 64 26 0 1 64 167 230 1.34e-22 90.1
MsaG008152 MtrunA17_Chr8g0379091 56.061 66 28 1 1 65 181 246 1.38e-22 90.5
MsaG008152 MtrunA17_Chr4g0001331 62.500 64 24 0 1 64 175 238 2.04e-22 89.7
MsaG008152 MtrunA17_Chr3g0091411 65.217 69 5 1 1 69 114 163 5.35e-22 86.3
MsaG008152 MtrunA17_Chr2g0304111 55.224 67 30 0 1 67 179 245 1.77e-21 87.0
MsaG008152 MtrunA17_Chr8g0346081 55.385 65 29 0 1 65 171 235 2.22e-21 86.7
MsaG008152 MtrunA17_Chr1g0150321 55.224 67 30 0 1 67 168 234 4.78e-21 85.9
MsaG008152 MtrunA17_Chr7g0244561 45.098 102 44 3 1 90 173 274 5.05e-21 85.5
MsaG008152 MtrunA17_Chr5g0428031 60.000 65 26 0 1 65 163 227 2.59e-20 83.6
MsaG008152 MtrunA17_Chr4g0063881 55.385 65 29 0 1 65 165 229 7.98e-20 82.4
MsaG008152 MtrunA17_Chr8g0346071 53.125 64 30 0 1 64 171 234 8.14e-20 82.4
MsaG008152 MtrunA17_Chr3g0091311 62.687 67 4 1 1 67 166 211 1.23e-19 80.1
MsaG008152 MtrunA17_Chr1g0205421 58.730 63 26 0 1 63 167 229 9.61e-19 79.3
MsaG008152 MtrunA17_Chr4g0069301 51.562 64 31 0 1 64 182 245 1.04e-18 79.3
MsaG008152 MtrunA17_Chr2g0302431 50.000 72 32 1 1 68 176 247 1.21e-18 79.0
MsaG008152 MtrunA17_Chr2g0333821 55.556 63 28 0 1 63 169 231 6.96e-18 77.0
MsaG008152 MtrunA17_Chr3g0091291 57.746 71 9 1 1 71 114 163 1.22e-17 73.9
MsaG008152 MtrunA17_Chr4g0074111 57.895 57 24 0 1 57 173 229 1.75e-17 75.5
MsaG008152 MtrunA17_Chr1g0188031 52.308 65 30 1 1 64 177 241 6.57e-17 74.3
MsaG008152 MtrunA17_Chr4g0072831 63.462 52 19 0 16 67 139 190 1.08e-16 73.2
MsaG008152 MtrunA17_Chr1g0166101 50.000 64 32 0 1 64 180 243 8.76e-16 70.9
MsaG008152 MtrunA17_Chr6g0453671 49.206 63 32 0 1 63 172 234 1.42e-15 70.1
MsaG008152 MtrunA17_Chr3g0132671 49.206 63 32 0 1 63 202 264 4.42e-15 68.9
MsaG008152 MtrunA17_Chr6g0468751 51.613 62 30 0 2 63 173 234 4.68e-15 68.9
MsaG008152 MtrunA17_Chr6g0453681 51.613 62 30 0 2 63 173 234 5.35e-15 68.9
MsaG008152 MtrunA17_Chr8g0380661 42.105 76 40 1 1 72 152 227 6.64e-13 62.4
MsaG008152 MtrunA17_Chr3g0131061 54.000 50 23 0 8 57 162 211 1.04e-11 59.3
MsaG008152 MtrunA17_Chr2g0277501 54.000 50 23 0 8 57 162 211 1.28e-11 58.9
MsaG008152 MtrunA17_Chr1g0162301 53.704 54 24 1 8 60 182 235 1.62e-11 58.9
MsaG008152 MtrunA17_Chr8g0385281 54.000 50 23 0 8 57 163 212 2.12e-11 58.5
MsaG008152 MtrunA17_Chr8g0374571 54.000 50 23 0 8 57 181 230 2.88e-11 58.2
MsaG008152 MtrunA17_Chr1g0176421 48.077 52 25 1 11 62 328 377 3.30e-11 58.2
MsaG008152 MtrunA17_Chr4g0045371 54.000 50 23 0 8 57 181 230 4.34e-11 57.8
MsaG008152 MtrunA17_Chr1g0190131 43.860 57 31 1 8 64 169 224 9.18e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008152 AT5G45820.1 66.154 65 22 0 1 65 167 231 5.29e-26 99.8
MsaG008152 AT5G07070.1 63.077 65 24 0 1 65 167 231 7.89e-25 96.7
MsaG008152 AT2G26980.2 58.108 74 31 0 1 74 170 243 1.42e-24 93.6
MsaG008152 AT2G26980.1 58.108 74 31 0 1 74 170 243 2.03e-24 95.1
MsaG008152 AT5G25110.1 61.194 67 26 0 1 67 198 264 2.31e-24 95.5
MsaG008152 AT2G26980.3 60.563 71 28 0 1 71 170 240 2.71e-24 95.1
MsaG008152 AT2G26980.4 60.563 71 28 0 1 71 180 250 2.95e-24 95.1
MsaG008152 AT2G26980.5 58.108 74 31 0 1 74 170 243 3.80e-24 94.7
MsaG008152 AT4G30960.1 61.765 68 25 1 1 68 179 245 4.35e-24 94.7
MsaG008152 AT1G30270.3 59.701 67 27 0 1 67 90 156 8.46e-24 93.2
MsaG008152 AT5G58380.1 61.538 65 25 0 1 65 167 231 1.06e-23 93.6
MsaG008152 AT1G30270.1 59.701 67 27 0 1 67 187 253 1.24e-23 93.6
MsaG008152 AT1G30270.2 59.701 67 27 0 1 67 187 253 1.55e-23 93.2
MsaG008152 AT2G38490.1 62.500 64 24 0 1 64 183 246 4.37e-23 91.7
MsaG008152 AT5G01810.1 62.500 72 23 1 1 68 167 238 5.70e-23 91.3
MsaG008152 AT5G01810.2 62.500 72 23 1 1 68 167 238 5.70e-23 91.3
MsaG008152 AT2G30360.1 51.899 79 36 1 1 77 178 256 6.93e-23 91.3
MsaG008152 AT5G21326.1 60.870 69 27 0 1 69 169 237 7.00e-23 91.3
MsaG008152 AT1G29230.1 58.462 65 27 0 1 65 229 293 7.62e-23 91.3
MsaG008152 AT5G35410.2 53.333 75 35 0 1 75 165 239 1.05e-22 90.1
MsaG008152 AT5G01810.3 62.500 72 23 1 1 68 167 238 1.13e-22 90.1
MsaG008152 AT5G10930.1 58.209 67 28 0 1 67 168 234 1.17e-22 90.5
MsaG008152 AT4G18700.1 55.385 65 29 0 1 65 181 245 1.19e-22 90.9
MsaG008152 AT5G45810.1 53.846 65 30 0 1 65 183 247 2.13e-22 90.1
MsaG008152 AT5G01820.1 60.938 64 25 0 1 64 177 240 2.63e-22 89.7
MsaG008152 AT1G01140.3 59.701 67 27 0 1 67 175 241 2.64e-22 89.7
MsaG008152 AT1G01140.1 59.701 67 27 0 1 67 175 241 2.68e-22 89.7
MsaG008152 AT2G34180.1 60.317 63 25 0 3 65 214 276 2.76e-22 89.7
MsaG008152 AT5G35410.1 53.333 75 35 0 1 75 165 239 3.01e-22 89.7
MsaG008152 AT2G25090.1 54.930 71 32 0 1 71 179 249 2.18e-21 87.4
MsaG008152 AT1G01140.2 56.522 69 28 1 1 67 175 243 7.17e-21 85.9
MsaG008152 AT4G14580.1 56.250 64 28 0 1 64 178 241 7.68e-21 85.5
MsaG008152 AT4G24400.2 58.462 65 27 0 1 65 163 227 8.23e-20 82.8
MsaG008152 AT4G24400.1 58.462 65 27 0 1 65 163 227 1.04e-19 82.4
MsaG008152 AT5G21222.1 52.113 71 34 0 3 73 171 241 5.61e-19 80.5
MsaG008152 AT5G21222.2 52.113 71 34 0 3 73 171 241 5.61e-19 80.5
MsaG008152 AT5G21222.4 52.113 71 34 0 3 73 171 241 5.61e-19 80.5
MsaG008152 AT5G21222.3 52.113 71 34 0 3 73 171 241 5.61e-19 80.5
MsaG008152 AT3G23000.1 53.125 64 30 0 1 64 181 244 8.49e-19 79.7
MsaG008152 AT5G57630.3 50.725 69 34 0 1 69 76 144 3.03e-18 77.8
MsaG008152 AT5G57630.1 50.725 69 34 0 1 69 164 232 7.30e-18 77.0
MsaG008152 AT5G57630.2 50.725 69 34 0 1 69 164 232 7.59e-18 77.0
MsaG008152 AT3G17510.2 54.839 62 28 0 1 62 96 157 3.57e-17 75.1
MsaG008152 AT3G17510.1 54.839 62 28 0 1 62 176 237 5.10e-17 74.7
MsaG008152 AT1G48260.2 53.226 62 29 0 1 62 82 143 4.15e-16 72.0
MsaG008152 AT1G48260.4 53.226 62 29 0 1 62 82 143 4.15e-16 72.0
MsaG008152 AT1G48260.3 53.226 62 29 0 1 62 82 143 4.15e-16 72.0
MsaG008152 AT1G48260.5 53.226 62 29 0 1 62 82 143 4.15e-16 72.0
MsaG008152 AT1G48260.1 53.226 62 29 0 1 62 167 228 5.33e-16 72.0
MsaG008152 AT3G29160.3 51.613 62 30 0 2 63 174 235 1.06e-15 70.9
MsaG008152 AT3G29160.1 51.613 62 30 0 2 63 174 235 1.31e-15 70.9
MsaG008152 AT3G29160.2 51.613 62 30 0 2 63 174 235 1.31e-15 70.9
MsaG008152 AT3G01090.2 51.613 62 30 0 2 63 196 257 4.40e-15 69.3
MsaG008152 AT3G01090.3 51.613 62 30 0 2 63 173 234 4.69e-15 69.3
MsaG008152 AT3G01090.1 51.613 62 30 0 2 63 173 234 4.69e-15 69.3
MsaG008152 AT5G39440.1 48.387 62 31 1 2 63 173 233 5.22e-14 66.2
MsaG008152 AT5G08590.2 54.000 50 23 0 8 57 86 135 8.73e-12 59.7
MsaG008152 AT5G08590.1 54.000 50 23 0 8 57 162 211 1.03e-11 59.7
MsaG008152 AT1G10940.1 49.180 61 27 1 8 64 162 222 1.12e-11 59.7
MsaG008152 AT1G10940.2 49.180 61 27 1 8 64 170 230 1.15e-11 59.7
MsaG008152 AT4G33950.2 53.704 54 24 1 8 60 179 232 2.05e-11 58.9
MsaG008152 AT4G33950.1 53.704 54 24 1 8 60 179 232 2.58e-11 58.5
MsaG008152 AT1G78290.3 50.909 55 27 0 3 57 157 211 4.57e-11 57.8
MsaG008152 AT1G78290.2 50.909 55 27 0 3 57 157 211 4.57e-11 57.8
MsaG008152 AT4G40010.1 63.415 41 15 0 8 48 162 202 8.48e-11 57.0
MsaG008152 AT1G60940.1 44.118 68 34 1 1 64 155 222 8.67e-11 57.0
MsaG008152 AT1G60940.2 44.118 68 34 1 1 64 155 222 8.67e-11 57.0

Find 15 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAATTCTGATCATAAAA+TGG 0.213112 2:+29221322 None:intergenic
CTTCGTGGAAAAGGTTATTA+TGG 0.271911 2:-29221406 MsaT008152.1:CDS
AGTAATCTTATATGTTCTTT+TGG 0.308749 2:-29221356 MsaT008152.1:CDS
ATCATAAAATGGATAGTAAC+TGG 0.363255 2:+29221333 None:intergenic
CTCCATTGTTTATATGATAG+AGG 0.404035 2:+29220899 None:intergenic
CCAGATGTTCTTCGTGGAAA+AGG 0.428802 2:-29221415 MsaT008152.1:CDS
GATAGAGGAGGCCGAGCTCT+TGG 0.445285 2:+29220914 None:intergenic
CACATTTGTATTCTCCCTTG+TGG 0.450429 2:+29221281 None:intergenic
CGAGCTCTTGGAGGTAAACT+TGG 0.466496 2:+29220926 None:intergenic
AAAGCTGATGTTTGGTCTTG+CGG 0.503864 2:-29221379 MsaT008152.1:CDS
GTAGCTCCAGATGTTCTTCG+TGG 0.526034 2:-29221421 MsaT008152.1:CDS
GGAGAATACAAATGTGCCCC+TGG 0.564288 2:-29221274 MsaT008152.1:intron
AGAGGAGGCCGAGCTCTTGG+AGG 0.601187 2:+29220917 None:intergenic
CAAGTTTACCTCCAAGAGCT+CGG 0.616184 2:-29220925 MsaT008152.1:CDS
CATTGTTTATATGATAGAGG+AGG 0.660985 2:+29220902 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 29220908 29221476 29220908 ID=MsaG008152
Chr2 mRNA 29220908 29221476 29220908 ID=MsaT008152.1;Parent=MsaG008152
Chr2 exon 29220908 29221002 29220908 ID=MsaT008152.1.exon2;Parent=MsaT008152.1
Chr2 CDS 29220908 29221002 29220908 ID=cds.MsaT008152.1;Parent=MsaT008152.1
Chr2 exon 29221275 29221476 29221275 ID=MsaT008152.1.exon1;Parent=MsaT008152.1
Chr2 CDS 29221275 29221476 29221275 ID=cds.MsaT008152.1;Parent=MsaT008152.1
Gene Sequence

>MsaT008152.1

ATGTTGCAAACAATGTGTAGAACTCTGATGTATGTAGCTCCAGATGTTCTTCGTGGAAAAGGTTATTATGGAGAAAAAGCTGATGTTTGGTCTTGCGGAGTAATCTTATATGTTCTTTTGGCCAGTTACTATCCATTTTATGATCAGAATTTAATGGAACTGTATCGAAAAATCCACAAGGGAGAATACAAATGTGCCCCTGGGCTTGATTTGTCTGTCTTTTTTGCACGTAATGATGATGATGAAATTGAAGACACCAAGTTTACCTCCAAGAGCTCGGCCTCCTCTATCATATAA
Protein sequence

>MsaT008152.1

MLQTMCRTLMYVAPDVLRGKGYYGEKADVWSCGVILYVLLASYYPFYDQNLMELYRKIHKGEYKCAPGLDLSVFFARNDDDEIEDTKFTSKSSASSII*