AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008196


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008196 MtrunA17_Chr2g0284481 48.936 376 158 5 1 352 2 367 1.95e-111 330
MsaG008196 MtrunA17_Chr8g0379701 44.663 356 178 7 3 349 2 347 5.53e-88 270
MsaG008196 MtrunA17_Chr2g0301521 74.286 140 36 0 95 234 1 140 1.88e-64 202
MsaG008196 MtrunA17_Chr5g0410881 44.681 188 98 3 8 189 27 214 9.14e-50 174
MsaG008196 MtrunA17_Chr4g0039891 43.617 188 93 3 11 189 10 193 3.42e-46 164
MsaG008196 MtrunA17_Chr7g0260331 41.667 180 103 1 12 189 48 227 5.36e-42 150
MsaG008196 MtrunA17_Chr6g0467041 45.263 190 85 6 4 187 14 190 1.07e-40 145
MsaG008196 MtrunA17_Chr8g0378061 47.191 178 80 5 14 187 23 190 8.70e-40 147
MsaG008196 MtrunA17_Chr1g0204741 40.761 184 101 3 9 189 36 214 6.07e-39 141
MsaG008196 MtrunA17_Chr3g0135831 42.135 178 96 4 12 186 45 218 5.76e-37 137
MsaG008196 MtrunA17_Chr2g0330821 43.094 181 94 3 13 191 79 252 6.50e-37 136
MsaG008196 MtrunA17_Chr1g0185131 41.579 190 87 6 7 189 24 196 7.80e-37 135
MsaG008196 MtrunA17_Chr4g0071051 43.169 183 86 4 12 189 100 269 8.34e-36 134
MsaG008196 MtrunA17_Chr8g0392001 40.500 200 113 3 11 204 20 219 2.00e-35 132
MsaG008196 MtrunA17_Chr5g0397881 61.053 95 34 2 11 102 21 115 1.70e-33 127
MsaG008196 MtrunA17_Chr6g0485571 37.895 190 109 3 4 191 2 184 2.28e-33 126
MsaG008196 MtrunA17_Chr8g0375041 41.916 167 76 5 12 160 8 171 2.05e-32 122
MsaG008196 MtrunA17_Chr5g0402291 54.000 100 44 1 13 110 9 108 1.45e-31 120
MsaG008196 MtrunA17_Chr5g0441531 57.609 92 37 1 13 102 25 116 2.43e-30 116
MsaG008196 MtrunA17_Chr1g0203601 55.319 94 40 1 13 104 18 111 8.87e-30 116
MsaG008196 MtrunA17_Chr7g0257331 46.729 107 55 1 13 117 22 128 3.45e-29 112
MsaG008196 MtrunA17_Chr3g0134031 56.522 92 38 1 13 102 24 115 4.13e-29 115
MsaG008196 MtrunA17_Chr4g0047421 40.936 171 86 4 12 167 32 202 3.13e-28 109
MsaG008196 MtrunA17_Chr6g0472421 55.435 92 39 1 13 102 33 124 5.35e-28 112
MsaG008196 MtrunA17_Chr4g0023771 54.651 86 31 4 1 82 2 83 1.25e-19 83.2
MsaG008196 MtrunA17_Chr1g0179321 58.065 62 24 1 11 70 47 108 5.25e-19 81.6
MsaG008196 MtrunA17_Chr2g0330841 34.815 135 75 3 55 189 31 152 1.99e-14 73.9
MsaG008196 MtrunA17_Chr1g0179311 26.515 132 92 1 60 191 3 129 1.00e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008196 AT5G45710.3 41.854 356 150 11 1 354 1 301 2.48e-65 211
MsaG008196 AT5G45710.1 41.854 356 150 11 1 354 1 301 2.48e-65 211
MsaG008196 AT4G18880.1 42.896 366 181 14 2 354 3 353 2.93e-63 207
MsaG008196 AT1G32330.2 34.384 349 180 12 8 319 32 368 1.22e-47 168
MsaG008196 AT1G32330.1 34.384 349 180 12 8 319 32 368 1.39e-47 168
MsaG008196 AT2G26150.1 41.667 180 100 2 12 189 43 219 2.70e-43 153
MsaG008196 AT2G26150.4 41.667 180 100 2 12 189 43 219 2.70e-43 153
MsaG008196 AT4G17750.1 41.885 191 98 2 12 189 51 241 4.60e-43 156
MsaG008196 AT4G13980.1 47.191 178 85 3 14 189 24 194 3.12e-42 153
MsaG008196 AT5G16820.1 40.957 188 101 3 9 189 23 207 3.49e-42 153
MsaG008196 AT5G16820.2 40.957 188 101 3 9 189 23 207 3.49e-42 153
MsaG008196 AT3G22830.1 41.935 186 102 2 12 191 60 245 4.92e-42 151
MsaG008196 AT3G02990.1 41.758 182 103 2 11 189 21 202 4.58e-41 150
MsaG008196 AT5G03720.2 32.234 273 159 8 8 273 50 303 4.77e-38 141
MsaG008196 AT5G03720.1 32.234 273 159 8 8 273 50 303 4.77e-38 141
MsaG008196 AT5G43840.1 43.316 187 85 5 7 189 11 180 2.35e-37 136
MsaG008196 AT1G67970.1 40.761 184 103 5 11 191 17 197 3.58e-37 137
MsaG008196 AT3G51910.1 39.344 183 91 3 9 189 25 189 1.79e-35 130
MsaG008196 AT3G24520.1 35.354 198 100 4 11 206 15 186 1.07e-33 127
MsaG008196 AT3G63350.1 37.234 188 95 3 13 191 28 201 5.14e-33 124
MsaG008196 AT5G54070.1 35.354 198 113 3 11 205 69 254 5.14e-32 123
MsaG008196 AT5G62020.1 54.955 111 46 3 1 107 9 119 5.48e-32 122
MsaG008196 AT2G41690.1 55.340 103 43 2 12 111 39 141 6.69e-31 117
MsaG008196 AT4G11660.1 57.609 92 37 1 13 102 59 150 2.26e-30 119
MsaG008196 AT1G46264.1 53.333 105 44 3 1 102 23 125 4.36e-30 118
MsaG008196 AT4G36990.1 55.319 94 40 1 13 104 14 107 4.30e-29 114
MsaG008196 AT4G18870.1 47.000 100 50 2 1 98 1 99 3.29e-21 92.8
MsaG008196 AT4G18870.1 51.136 88 41 1 13 98 146 233 8.52e-21 91.7
MsaG008196 AT5G45710.4 33.077 260 119 10 95 354 1 205 4.11e-18 83.2
MsaG008196 AT5G45710.2 33.077 260 119 10 95 354 1 205 4.11e-18 83.2
MsaG008196 AT2G26150.3 31.092 119 79 1 71 189 49 164 6.60e-16 77.8
MsaG008196 AT2G26150.2 31.092 119 79 1 71 189 49 164 6.60e-16 77.8

Find 58 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTATAACCAGTAGTAAT+TGG 0.175524 2:-30058579 None:intergenic
GTTACAATAGGTTGTTCTTT+TGG 0.207514 2:-30058552 None:intergenic
CTGGTTATAATGATGAATTT+TGG 0.212791 2:+30058589 MsaT008196.1:CDS
TTACCAAAAGTACTATTTCT+AGG 0.227594 2:-30058210 None:intergenic
TAGAAAAGGAGGAATCTTAT+TGG 0.235062 2:-30056663 None:intergenic
CATCGTTCTATTTCACTTAG+TGG 0.275481 2:-30057943 None:intergenic
CGAGTCATCAACGGAAGTTT+TGG 0.324868 2:+30058338 MsaT008196.1:CDS
GAAAAGTTTCTTCAGAAAGA+TGG 0.342458 2:+30057803 MsaT008196.1:CDS
ACCTCTGGCTCAGTTGAAAT+AGG 0.345467 2:-30058426 None:intergenic
ATTCTAATTCCATTGTTTCC+TGG 0.353748 2:+30056711 MsaT008196.1:CDS
TATCCTAGAAATAGTACTTT+TGG 0.357278 2:+30058207 MsaT008196.1:CDS
TGCAAAGAATGGCTATGAAC+AGG 0.400244 2:-30057892 None:intergenic
GTAATTGGGAATGTTACAAT+AGG 0.418851 2:-30058564 None:intergenic
GTAATCATTGCTGCTAATCT+TGG 0.427900 2:+30058447 MsaT008196.1:CDS
AAGGATGAATCGGAAAATAT+CGG 0.429889 2:+30058624 MsaT008196.1:CDS
CTGAGATTGAGTGTCTTAAC+TGG 0.431217 2:+30058151 MsaT008196.1:CDS
TCAGGACTAACAACTACATT+TGG 0.442595 2:-30058402 None:intergenic
AAAAGTTTCTTCAGAAAGAT+GGG 0.446487 2:+30057804 MsaT008196.1:CDS
GTAACATTCCCAATTACTAC+TGG 0.454277 2:+30058570 MsaT008196.1:CDS
TTCTTATGCTTAAAATATCT+AGG 0.456469 2:-30056794 None:intergenic
CATTATAACCAGTAGTAATT+GGG 0.464359 2:-30058578 None:intergenic
TGAAAATCTTATAAATGAAA+AGG 0.464497 2:+30057984 MsaT008196.1:CDS
ACATTCTCTTCGCTTTCTCT+TGG 0.469302 2:-30058276 None:intergenic
CAGTTAAGACACTCAATCTC+AGG 0.469530 2:-30058150 None:intergenic
GGCTCAGTTGAAATAGGATC+AGG 0.472603 2:-30058420 None:intergenic
ACAACACTTAAAGGATGAAT+CGG 0.488019 2:+30058614 MsaT008196.1:CDS
ACTTCTACCGTTAGCACTCC+AGG 0.491458 2:-30056729 None:intergenic
AAGAAAATACGCAGAGAGAA+AGG 0.498599 2:+30058181 MsaT008196.1:CDS
TTAGCAGCAATGATTACCTC+TGG 0.546847 2:-30058441 None:intergenic
AAGAGGTCAACCACATCTGA+TGG 0.548717 2:+30057849 MsaT008196.1:CDS
ACTTCAGACAAAGGAAGAAG+AGG 0.555344 2:+30058023 MsaT008196.1:CDS
TCTTGGTGCAACTGAGGAAG+CGG 0.565485 2:+30058464 MsaT008196.1:CDS
ACAGAAACAACACTTGGCTC+AGG 0.569479 2:-30058513 None:intergenic
TCGGAAAATATCGGTAAGCA+TGG 0.571197 2:+30058633 MsaT008196.1:CDS
ATTGTTTCCTGGAGTGCTAA+CGG 0.572222 2:+30056722 MsaT008196.1:CDS
CATGTGTATTCTGCAAAGAA+TGG 0.576636 2:-30057903 None:intergenic
ATCTGTTCTCTCATTAAACA+TGG 0.581426 2:+30058299 MsaT008196.1:CDS
GGCTCAGAAGCAACAAGGTG+AGG 0.585499 2:-30058381 None:intergenic
TCTTCTTCAACTTCAGACAA+AGG 0.589139 2:+30058014 MsaT008196.1:CDS
ATCTGATGGAGAATATCCAT+AGG 0.604051 2:+30057863 MsaT008196.1:CDS
GGTCTGATGGAAGATGGTCA+TGG 0.606080 2:+30056635 None:intergenic
TGCAGAATACACATGGACAA+GGG 0.609195 2:+30057911 MsaT008196.1:CDS
TCCTATTTCAACTGAGCCAG+AGG 0.611428 2:+30058425 MsaT008196.1:CDS
GCAGAATACACATGGACAAG+GGG 0.613563 2:+30057912 MsaT008196.1:CDS
TGGATATTCTCCATCAGATG+TGG 0.621608 2:-30057859 None:intergenic
TTGCAGAATACACATGGACA+AGG 0.625364 2:+30057910 MsaT008196.1:CDS
TCTGATGGAGAATATCCATA+GGG 0.625711 2:+30057864 MsaT008196.1:CDS
GAATCTGCTCGAGTCATCAA+CGG 0.627558 2:+30058329 MsaT008196.1:CDS
GACACAGTTACATTACTCAA+AGG 0.628646 2:+30058059 MsaT008196.1:CDS
AGCTAGCACTCAAAGTCCTG+TGG 0.629789 2:+30058236 MsaT008196.1:CDS
CATTCTTTGCAGAATACACA+TGG 0.632095 2:+30057904 MsaT008196.1:CDS
CTGATGGAAGATGGTCATGG+TGG 0.633034 2:+30056638 None:intergenic
CATTTGGCTCAGAAGCAACA+AGG 0.635497 2:-30058386 None:intergenic
TGCTAATCTTGGTGCAACTG+AGG 0.647204 2:+30058458 MsaT008196.1:CDS
GTTAGCACTCCAGGAAACAA+TGG 0.651356 2:-30056720 None:intergenic
TCTGGGACAGAAACAACACT+TGG 0.664859 2:-30058519 None:intergenic
CACTAAGTGAAATAGAACGA+TGG 0.678895 2:+30057944 MsaT008196.1:CDS
AAAATACGCAGAGAGAAAGG+TGG 0.696471 2:+30058184 MsaT008196.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 30056641 30058659 30056641 ID=MsaG008196
Chr2 mRNA 30056641 30058659 30056641 ID=MsaT008196.1;Parent=MsaG008196
Chr2 exon 30056641 30056850 30056641 ID=MsaT008196.1.exon1;Parent=MsaT008196.1
Chr2 CDS 30056641 30056850 30056641 ID=cds.MsaT008196.1;Parent=MsaT008196.1
Chr2 exon 30057796 30058659 30057796 ID=MsaT008196.1.exon2;Parent=MsaT008196.1
Chr2 CDS 30057796 30058659 30057796 ID=cds.MsaT008196.1;Parent=MsaT008196.1
Gene Sequence

>MsaT008196.1

ATGGAAGATGGTCATGGTGGTTCCAATAAGATTCCTCCTTTTCTAAAAAACACTTATGAAATGGTTGAAGATTCTAATTCCATTGTTTCCTGGAGTGCTAACGGTAGAAGTTTCATTGTTTCGAATCCATTCGATTTTGAAAAAGATTTGTTACCTAGATATTTTAAGCATAAGAACTTCTCAAGTTTCAAGAGACAACTGAATACCTATGGATTTAGAAAAGTTTCTTCAGAAAGATGGGAATTCGCAAACGATAATTTTGTAAGAGGTCAACCACATCTGATGGAGAATATCCATAGGGAAAAACCTGTTCATAGCCATTCTTTGCAGAATACACATGGACAAGGGGCTTCTACTCCACTAAGTGAAATAGAACGATGGAATTTGAACGCTATAATTGAAAATCTTATAAATGAAAAGGAACATATTCTTCTTCAACTTCAGACAAAGGAAGAAGAGGAGAAAATTCACGAGACACAGTTACATTACTCAAAGGAGCGTCTTAAAGTGTTTGAACAGAAGCTGCAAAGTATGCTCTCTTTTGTTGGTCAAGCGTTGCGCAAACCTGAGATTGAGTGTCTTAACTGGTCATCGAAAGAAAATACGCAGAGAGAAAGGTGGTATCCTAGAAATAGTACTTTTGGTAATGAAGCTAGCACTCAAAGTCCTGTGGAAAATTCTCAAGTATTACCAAGAGAAAGCGAAGAGAATGTATCTGTTCTCTCATTAAACATGGAACGATTGAATCTGCTCGAGTCATCAACGGAAGTTTTGGAGAGTATCGGAATTGGAATTCCTCACCTTGTTGCTTCTGAGCCAAATGTAGTTGTTAGTCCTGATCCTATTTCAACTGAGCCAGAGGTAATCATTGCTGCTAATCTTGGTGCAACTGAGGAAGCGGTAATCAATGATCATGATTTCGTATCACCTGAGCCAAGTGTTGTTTCTGTCCCAGATAACGTTGCCCCAAAAGAACAACCTATTGTAACATTCCCAATTACTACTGGTTATAATGATGAATTTTGGGAACAACACTTAAAGGATGAATCGGAAAATATCGGTAAGCATGGTTAA

Protein sequence

>MsaT008196.1

MEDGHGGSNKIPPFLKNTYEMVEDSNSIVSWSANGRSFIVSNPFDFEKDLLPRYFKHKNFSSFKRQLNTYGFRKVSSERWEFANDNFVRGQPHLMENIHREKPVHSHSLQNTHGQGASTPLSEIERWNLNAIIENLINEKEHILLQLQTKEEEEKIHETQLHYSKERLKVFEQKLQSMLSFVGQALRKPEIECLNWSSKENTQRERWYPRNSTFGNEASTQSPVENSQVLPRESEENVSVLSLNMERLNLLESSTEVLESIGIGIPHLVASEPNVVVSPDPISTEPEVIIAANLGATEEAVINDHDFVSPEPSVVSVPDNVAPKEQPIVTFPITTGYNDEFWEQHLKDESENIGKHG*