AlfalfaGEDB | MsaG008253

M. sativa ssp. caerulea / MsaG008253

KEGG: K13464
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009791 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010556 GO:0010558 GO:0010605 GO:0010646 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045934 GO:0048229 GO:0048367 GO:0048519 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0090567 GO:0098542 GO:0140110 GO:1900067 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000022 GO:2001141
PFAM: PF06200.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008253	XP_003595354.1	97.802	182	4	0	1	182	72	253	6.23e-129	372

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008253	sp\|A2YNP2\|TI10B_ORYSI	35.938	192	86	5	1	174	57	229	1.58e-27	106

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008253	G7IIQ4	97.802	182	4	0	1	182	72	253	2.98e-129	372

TFs/TRs:

Gene ID	Type	Classification
MsaG008253	TF	Tify

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG003265	MsaG008253	0.815580	1.489031e-51	2.920980e-49
MsaG004525	MsaG008253	0.811925	9.418744e-51	1.686061e-48
MsaG006279	MsaG008253	0.810949	1.530940e-50	2.675621e-48
MsaG006832	MsaG008253	0.811695	1.056349e-50	1.880273e-48
MsaG008253	MsaG010958	0.813826	3.627036e-51	6.807702e-49
MsaG008253	MsaG015664	0.808357	5.486957e-50	9.006137e-48
MsaG008253	MsaG016670	0.836652	1.510733e-56	5.317473e-54
MsaG008253	MsaG023847	0.810405	2.004746e-50	3.457598e-48
MsaG008253	MsaG029761	0.800926	1.918302e-48	2.648394e-46
MsaG008253	MsaG041694	0.873408	3.096967e-67	4.153193e-64
MsaG008253	MsaG045313	0.819225	2.270529e-52	4.892699e-50
MsaG008253	MsaG019850	0.813975	3.363282e-51	6.336201e-49
MsaG008253	MsaG027739	0.815678	1.416113e-51	2.784902e-49
MsaG008253	MsaG025853	0.824792	1.181074e-53	2.954066e-51
MsaG008253	MsaG035088	0.846496	3.943202e-59	1.898546e-56
MsaG008253	MsaG041261	0.809208	3.616596e-50	6.058893e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008253	MtrunA17_Chr2g0301131	97.802	182	4	0	1	182	72	253	5.73e-133	372
MsaG008253	MtrunA17_Chr5g0399851	45.513	156	47	6	31	174	10	139	1.33e-35	121
MsaG008253	MtrunA17_Chr5g0399881	44.118	170	49	5	28	181	25	164	2.15e-34	119
MsaG008253	MtrunA17_Chr4g0070271	41.799	189	49	6	1	180	67	203	3.62e-34	119
MsaG008253	MtrunA17_Chr5g0399861	44.785	163	43	6	28	174	25	156	8.67e-34	117
MsaG008253	MtrunA17_Chr4g0070241	55.072	69	25	3	1	69	1	63	9.37e-16	68.9
MsaG008253	MtrunA17_Chr2g0302041	32.031	128	62	5	41	162	58	166	2.55e-14	68.2
MsaG008253	MtrunA17_Chr8g0344481	34.109	129	66	3	37	165	101	210	3.39e-13	65.5
MsaG008253	MtrunA17_Chr1g0161031	30.460	174	67	4	39	169	172	334	2.41e-12	64.3
MsaG008253	MtrunA17_Chr4g0069721	28.682	129	73	3	37	162	41	153	8.38e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008253	AT1G74950.1	39.610	154	53	4	38	174	116	246	7.02e-26	100
MsaG008253	AT1G19180.2	39.041	146	59	4	38	174	57	181	4.43e-23	91.3
MsaG008253	AT1G19180.1	39.041	146	59	4	38	174	123	247	1.24e-22	91.7
MsaG008253	AT1G19180.3	39.041	146	59	4	38	174	123	247	1.24e-22	91.7
MsaG008253	AT1G74950.2	39.535	129	44	3	38	155	116	221	1.27e-19	82.8
MsaG008253	AT1G72450.1	37.795	127	61	3	40	166	105	213	1.25e-16	75.9
MsaG008253	AT1G17380.2	35.211	142	58	5	37	168	65	182	8.87e-15	70.5
MsaG008253	AT1G17380.1	35.211	142	58	5	37	168	94	211	1.83e-14	69.7
MsaG008253	AT5G20900.1	31.967	122	68	2	41	162	57	163	2.15e-12	62.8
MsaG008253	AT3G17860.3	31.646	158	73	6	40	166	144	297	4.25e-11	60.8
MsaG008253	AT3G17860.4	31.646	158	73	6	40	166	144	297	4.25e-11	60.8
MsaG008253	AT3G17860.1	31.646	158	73	6	40	166	177	330	5.08e-11	60.8
MsaG008253	AT3G17860.2	31.646	158	73	6	40	166	164	317	6.18e-11	60.5

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCTTCATTTCCTCCTAATT+TGG	0.255924	2:-31231655	MsaT008253.1:CDS
AGCCTCGCTTCATCGGTTTC+TGG	0.260318	2:-31231400	MsaT008253.1:CDS
GCTGGCTTATTAACTTGATA+TGG	0.264533	2:+31231219	None:intergenic
CATGAGCTCATGTGCTTTAT+CGG	0.271727	2:+31231743	None:intergenic
AGCGAGGCTTTCCTCATAAT+TGG	0.283392	2:+31231414	None:intergenic
ACCGAAGTTCTGACCAAATT+AGG	0.285058	2:+31231642	None:intergenic
ATTGTCAATTGTGCAGCTTT+AGG	0.328266	2:+31231807	None:intergenic
ACAAAAGAACCTTACAACTA+TGG	0.341905	2:-31232394	MsaT008253.1:CDS
CCGGTGCCGGTGGCAGTACT+AGG	0.356261	2:+31231579	None:intergenic
CATGGTTGGAAACAAGTTCA+TGG	0.390560	2:+31232431	None:intergenic
AAGAAATTACCGCAAAGATT+AGG	0.403918	2:+31231522	None:intergenic
AATGATTTGCAGTGCTATCA+AGG	0.444008	2:-31231832	MsaT008253.1:intron
CCTTCCAGACCTAATCTTTG+CGG	0.448657	2:-31231531	MsaT008253.1:intron
ATTACCGCAAAGATTAGGTC+TGG	0.470927	2:+31231527	None:intergenic
AGCTCATGTGCTTTATCGGC+AGG	0.475762	2:+31231747	None:intergenic
GAGTCCATGTCATGGCTTGT+GGG	0.483230	2:-31231195	MsaT008253.1:CDS
TCCTAATTTGGTCAGAACTT+CGG	0.497883	2:-31231643	MsaT008253.1:CDS
GCGCAGATCTGCCAATTATG+AGG	0.500927	2:-31231425	MsaT008253.1:intron
TAGGTCTGGAAGGCACTTGA+AGG	0.506440	2:+31231541	None:intergenic
TGAGGAAAGCCTCGCTTCAT+CGG	0.508146	2:-31231407	MsaT008253.1:CDS
ATGTTCACGGTAGGAACAAT+CGG	0.508733	2:+31231606	None:intergenic
TCGGTTTCTGGAGAAGAGAA+AGG	0.512539	2:-31231388	MsaT008253.1:CDS
CATGAACTTGTTTCCAACCA+TGG	0.520010	2:-31232430	MsaT008253.1:CDS
CGGTGCCGGTGGCAGTACTA+GGG	0.531615	2:+31231580	None:intergenic
CGATAAAGCACATGAGCTCA+TGG	0.531706	2:-31231742	MsaT008253.1:CDS
TGAGCAAATCCATAGTTGTA+AGG	0.532508	2:+31232385	None:intergenic
TTCATTAATAGAGCCGGTGC+CGG	0.533591	2:+31231566	None:intergenic
TGAGTCCATGTCATGGCTTG+TGG	0.539136	2:-31231196	MsaT008253.1:CDS
GAATTGTTCAAAGCAGCTTG+TGG	0.542652	2:+31232360	None:intergenic
CCTAGTACTGCCACCGGCAC+CGG	0.542742	2:-31231579	MsaT008253.1:CDS
AAGGTGTTCATTAATAGAGC+CGG	0.555443	2:+31231560	None:intergenic
TGCACCCACAAGCCATGACA+TGG	0.562173	2:+31231191	None:intergenic
ACCAAATTAGGAGGAAATGA+AGG	0.565826	2:+31231654	None:intergenic
AGCCATGACATGGACTCAGC+TGG	0.572954	2:+31231201	None:intergenic
AATTAGGAGGAAATGAAGGC+TGG	0.580229	2:+31231658	None:intergenic
CCGCAAAGATTAGGTCTGGA+AGG	0.596635	2:+31231531	None:intergenic
ATTAATAGAGCCGGTGCCGG+TGG	0.600813	2:+31231569	None:intergenic
ATCATCCCTAGTACTGCCAC+CGG	0.601610	2:-31231585	MsaT008253.1:CDS
CTCCAGAAACCGATGAAGCG+AGG	0.614975	2:+31231398	None:intergenic
AGTACTAGGGATGATGTTCA+CGG	0.617551	2:+31231593	None:intergenic
TTCTGGAGAAGAGAAAGGAT+AGG	0.619484	2:-31231383	MsaT008253.1:intron
GAAGTTCTGACCAAATTAGG+AGG	0.621959	2:+31231645	None:intergenic
ATGATTTGCAGTGCTATCAA+GGG	0.622638	2:-31231831	MsaT008253.1:intron
ATTAACTTGATATGGTGCGT+TGG	0.634047	2:+31231227	None:intergenic
AGCCAGCTGAGTCCATGTCA+TGG	0.640187	2:-31231203	MsaT008253.1:CDS
CTAGGGATGATGTTCACGGT+AGG	0.671499	2:+31231597	None:intergenic
CACGGTAGGAACAATCGGAG+TGG	0.691712	2:+31231611	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	31231173	31232451	31231173	ID=MsaG008253
Chr2	mRNA	31231173	31232451	31231173	ID=MsaT008253.1;Parent=MsaG008253
Chr2	exon	31231173	31231257	31231173	ID=MsaT008253.1.exon4;Parent=MsaT008253.1
Chr2	CDS	31231173	31231257	31231173	ID=cds.MsaT008253.1;Parent=MsaT008253.1
Chr2	exon	31231384	31231441	31231384	ID=MsaT008253.1.exon3;Parent=MsaT008253.1
Chr2	CDS	31231384	31231441	31231384	ID=cds.MsaT008253.1;Parent=MsaT008253.1
Chr2	exon	31231532	31231842	31231532	ID=MsaT008253.1.exon2;Parent=MsaT008253.1
Chr2	CDS	31231532	31231842	31231532	ID=cds.MsaT008253.1;Parent=MsaT008253.1
Chr2	exon	31232357	31232451	31232357	ID=MsaT008253.1.exon1;Parent=MsaT008253.1
Chr2	CDS	31232357	31232451	31232357	ID=cds.MsaT008253.1;Parent=MsaT008253.1

Gene Sequence

>MsaT008253.1

ATGAACTTGTTTCCAACCATGGAAAACAATTTGACACAAAAGAACCTTACAACTATGGATTTGCTCACTCCACAAGCTGCTTTGAACAATTCCAATGCTATCAAGGGACCTAAAGCTGCACAATTGACAATGTTTTATAACGGTCAAGTTATTGTATTCGATGATTTTCCTGCCGATAAAGCACATGAGCTCATGGCTTTTGCTAACAAAGGAATCTCTCAAAGTCAGAACAATTCTGTGTACACTTACACACAGAGCCAGCCTTCATTTCCTCCTAATTTGGTCAGAACTTCGGTTAACACAACCACTCCGATTGTTCCTACCGTGAACATCATCCCTAGTACTGCCACCGGCACCGGCTCTATTAATGAACACCTTCAAGTGCCTTCCAGACCTAATCTTTGCGATCTGCCAATTATGAGGAAAGCCTCGCTTCATCGGTTTCTGGAGAAGAGAAAGGATAGAATTGCTGCCAACGCACCATATCAAGTTAATAAGCCAGCTGAGTCCATGTCATGGCTTGTGGGTGCAAAATCAACTCAAATTTGA

Protein sequence

>MsaT008253.1

MNLFPTMENNLTQKNLTTMDLLTPQAALNNSNAIKGPKAAQLTMFYNGQVIVFDDFPADKAHELMAFANKGISQSQNNSVYTYTQSQPSFPPNLVRTSVNTTTPIVPTVNIIPSTATGTGSINEHLQVPSRPNLCDLPIMRKASLHRFLEKRKDRIAANAPYQVNKPAESMSWLVGAKSTQI*