AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008335

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008335 MtrunA17_Chr7g0274481 94.444 720 39 1 4 723 8 726 0.0 1315
MsaG008335 MtrunA17_Chr5g0397691 92.962 611 35 3 1 607 1 607 0.0 1063
MsaG008335 MtrunA17_Chr5g0397681 89.796 49 5 0 626 674 1 49 3.35e-24 96.3
MsaG008335 MtrunA17_Chr7g0238631 38.095 105 64 1 4 107 62 166 4.07e-15 79.0
MsaG008335 MtrunA17_Chr7g0238631 31.008 129 78 2 4 132 114 231 4.26e-11 66.2
MsaG008335 MtrunA17_Chr8g0362231 34.021 97 64 0 3 99 22 118 4.85e-13 72.0
MsaG008335 MtrunA17_Chr3g0139671 34.286 105 68 1 4 107 34 138 7.87e-13 72.4
MsaG008335 MtrunA17_Chr3g0139671 36.471 85 54 0 4 88 86 170 2.70e-12 70.5
MsaG008335 MtrunA17_Chr1g0157731 34.343 99 64 1 4 101 40 138 1.14e-12 71.6
MsaG008335 MtrunA17_Chr5g0416341 33.945 109 68 1 3 111 26 130 1.18e-12 70.9
MsaG008335 MtrunA17_Chr4g0058991 37.647 85 53 0 4 88 210 294 2.80e-11 66.6
MsaG008335 MtrunA17_Chr5g0397841 37.349 83 52 0 6 88 113 195 5.01e-11 65.9
MsaG008335 MtrunA17_Chr6g0480581 35.632 87 56 0 2 88 3 89 5.47e-11 63.5
MsaG008335 MtrunA17_Chr6g0460661 34.783 92 60 0 6 97 19 110 5.77e-11 61.6
MsaG008335 MtrunA17_Chr6g0460671 31.959 97 66 0 4 100 100 196 6.57e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008335 AT1G09770.1 75.346 722 172 4 4 722 8 726 0.0 1048
MsaG008335 AT5G02320.1 37.143 105 65 1 4 107 76 180 1.70e-14 77.4
MsaG008335 AT5G02320.1 34.118 85 56 0 4 88 128 212 8.74e-11 65.5
MsaG008335 AT5G02320.2 37.143 105 65 1 4 107 76 180 1.70e-14 77.4
MsaG008335 AT5G02320.2 34.118 85 56 0 4 88 128 212 8.74e-11 65.5
MsaG008335 AT5G02320.3 37.143 105 65 1 4 107 16 120 1.99e-14 77.0
MsaG008335 AT3G18100.1 34.375 96 63 0 4 99 547 642 6.40e-14 76.3
MsaG008335 AT3G18100.1 36.082 97 60 2 6 101 498 593 2.45e-11 67.8
MsaG008335 AT3G18100.3 34.375 96 63 0 4 99 499 594 1.06e-13 75.5
MsaG008335 AT3G18100.3 36.082 97 60 2 6 101 450 545 4.02e-11 67.0
MsaG008335 AT3G09370.2 36.190 105 66 1 4 107 84 188 1.15e-13 74.7
MsaG008335 AT3G18100.4 34.375 96 63 0 4 99 386 481 1.32e-13 75.1
MsaG008335 AT3G18100.4 36.082 97 60 2 6 101 337 432 5.29e-11 66.6
MsaG008335 AT3G09370.1 36.190 105 66 1 4 107 79 183 1.35e-13 74.3
MsaG008335 AT5G11510.3 37.374 99 61 1 4 101 30 128 1.36e-13 75.1
MsaG008335 AT5G11510.1 37.374 99 61 1 4 101 30 128 1.36e-13 75.1
MsaG008335 AT5G11510.4 37.374 99 61 1 4 101 30 128 1.36e-13 75.1
MsaG008335 AT3G18100.2 34.375 96 63 0 4 99 334 429 1.62e-13 74.7
MsaG008335 AT3G18100.2 36.082 97 60 2 6 101 285 380 6.66e-11 66.2
MsaG008335 AT3G09370.3 36.190 105 66 1 4 107 92 196 1.71e-13 74.3
MsaG008335 AT5G11510.2 37.374 99 61 1 4 101 30 128 2.30e-13 74.3
MsaG008335 AT5G11510.5 37.374 99 61 1 4 101 30 128 2.63e-13 73.9
MsaG008335 AT4G18770.1 38.824 85 52 0 4 88 218 302 9.25e-13 71.2
MsaG008335 AT4G32730.5 35.354 99 63 1 4 101 36 134 1.39e-12 72.0
MsaG008335 AT4G32730.4 35.354 99 63 1 4 101 36 134 1.39e-12 72.0
MsaG008335 AT4G32730.3 35.354 99 63 1 4 101 36 134 1.39e-12 72.0
MsaG008335 AT4G32730.2 35.354 99 63 1 4 101 36 134 1.39e-12 72.0
MsaG008335 AT4G32730.1 35.354 99 63 1 4 101 36 134 2.43e-12 70.9
MsaG008335 AT4G33450.1 32.990 97 65 0 4 100 20 116 5.73e-12 67.0
MsaG008335 AT2G02820.2 30.579 121 70 1 3 109 30 150 8.23e-12 68.6
MsaG008335 AT2G02820.1 30.579 121 70 1 3 109 30 150 1.27e-11 68.2
MsaG008335 AT4G00540.2 37.500 112 61 2 4 106 47 158 1.29e-11 67.8
MsaG008335 AT4G00540.1 37.500 112 61 2 4 106 45 156 1.80e-11 67.4
MsaG008335 AT1G14350.2 31.959 97 66 0 3 99 25 121 2.42e-11 67.0
MsaG008335 AT1G14350.1 31.959 97 66 0 3 99 25 121 2.42e-11 67.0
MsaG008335 AT2G02820.3 30.579 121 70 1 3 109 30 150 2.56e-11 66.2
MsaG008335 AT5G40360.1 35.294 85 55 0 4 88 159 243 2.88e-11 66.2
MsaG008335 AT5G17800.1 34.343 99 65 0 2 100 92 190 3.75e-11 65.5
MsaG008335 AT1G17950.1 31.313 99 68 0 2 100 4 102 4.43e-11 64.3
MsaG008335 AT4G38620.1 31.655 139 84 5 4 138 15 146 5.74e-11 64.7

Find 208 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTCTTTCCAACTGTTTC+TGG 0.013419 2:+32738860 None:intergenic
TTCTGGGTCATTCAGCTTCT+TGG 0.153986 2:+32738877 None:intergenic
TGCCAGCGCCATACCACTTC+TGG 0.197747 2:+32738445 None:intergenic
ATTTCATAACAGCAGCTTTC+AGG 0.225007 2:+32739668 None:intergenic
CAGCATCTCTGGAGCTTATT+AGG 0.228626 2:-32738076 MsaT008335.1:CDS
GGAGTAAGGCCTGCACTTCC+AGG 0.240853 2:+32738467 None:intergenic
TTGGGTGTCTGTGGATAATT+AGG 0.241982 2:+32738707 None:intergenic
CATGATGGAAGCTGAAAATT+TGG 0.248037 2:-32738624 MsaT008335.1:CDS
AAAGGAGCTTCCAGCCTTAC+TGG 0.273790 2:-32737973 MsaT008335.1:CDS
GTTCTCTGAGGAGTTCTTAA+AGG 0.283026 2:+32738671 None:intergenic
AAAGCTGCTGTTATGAAATA+TGG 0.287568 2:-32739664 MsaT008335.1:CDS
GAATTGGTTGCATCACTATC+TGG 0.302904 2:+32738246 None:intergenic
GGTCGCGCAGGCTTCGATTC+AGG 0.310060 2:+32739325 None:intergenic
AGTCCACTCAGTCTTCTTAA+TGG 0.314486 2:+32739552 None:intergenic
GTTTGAGAAAAGGCCTCCTT+TGG 0.314999 2:-32739074 MsaT008335.1:CDS
CCCTGTCATAGAAGATTATC+AGG 0.315605 2:-32737862 MsaT008335.1:CDS
CCATTGCCTCACGAAGATAC+TGG 0.317289 2:+32737129 None:intergenic
TGGGGTCACTCCTAAGAAAA+AGG 0.319124 2:-32738531 MsaT008335.1:CDS
ATCTCTTTCCAACTGTTTCT+GGG 0.330928 2:+32738861 None:intergenic
TCTTCAGCATCTTCTTGAAT+TGG 0.331097 2:+32738230 None:intergenic
ATCTCGTCGGCTTGCTCAAT+AGG 0.342238 2:+32738002 None:intergenic
TCAAGTTCTTCTATAGTAGT+AGG 0.344179 2:+32739001 None:intergenic
TAGGCCTCCTCCAGCATCTC+TGG 0.367513 2:-32738087 MsaT008335.1:CDS
CAGAAATTCCGTTTGAGAAA+AGG 0.368252 2:-32739084 MsaT008335.1:CDS
GCTTGAGGAGATAGCAAATA+AGG 0.373696 2:-32737928 MsaT008335.1:CDS
CCTTGACGCCACTTGTGTTA+AGG 0.386161 2:-32739410 MsaT008335.1:CDS
TCTCGTCGGCTTGCTCAATA+GGG 0.387795 2:+32738003 None:intergenic
AGGCCCATTTGCTGCTCGTT+TGG 0.388358 2:+32737890 None:intergenic
TCAGATTTCCGATCAAGAAT+TGG 0.389053 2:-32738816 MsaT008335.1:CDS
GCAGGCTTCGATTCAGGATT+CGG 0.393602 2:+32739331 None:intergenic
CCAAAATGAGCATAGGTGTT+TGG 0.397192 2:-32739711 None:intergenic
CTACGAAGGGAACTCCTCTC+AGG 0.399722 2:-32738397 MsaT008335.1:CDS
AGGCTGCTGGGATTGATATA+AGG 0.409355 2:-32739144 MsaT008335.1:CDS
TCTGCAACCCCTGGAAGTGC+AGG 0.411721 2:-32738476 MsaT008335.1:CDS
AGAGATGCTGGAGGAGGCCT+AGG 0.415805 2:+32738089 None:intergenic
CATGGTCTATCAAGTGTTGC+TGG 0.417267 2:-32737020 MsaT008335.1:CDS
GGATCCAAACGAGCAGCAAA+TGG 0.417377 2:-32737894 MsaT008335.1:CDS
TTGATAGACCATGTGTGTTA+CGG 0.418408 2:+32737030 None:intergenic
GCTACATATCAATGAAGATA+TGG 0.420115 2:-32738369 MsaT008335.1:CDS
TAAGGCAGCGGAGGAGGAAA+AGG 0.424744 2:-32739126 MsaT008335.1:CDS
TGATAGACCATGTGTGTTAC+GGG 0.426495 2:+32737031 None:intergenic
TTGGATGAAATTGCCAAGTT+GGG 0.429250 2:-32738797 MsaT008335.1:CDS
AGCTTTCAGGATTTCATCCT+CGG 0.436489 2:+32739681 None:intergenic
CAGTATCTTCGTGAGGCAAT+GGG 0.437430 2:-32737128 MsaT008335.1:CDS
ATTGGATGAAATTGCCAAGT+TGG 0.444138 2:-32738798 MsaT008335.1:CDS
AAGAACTTGAGGGGAAAAGA+AGG 0.444403 2:-32738988 MsaT008335.1:CDS
GCCAGCGCCATACCACTTCT+GGG 0.445237 2:+32738446 None:intergenic
GCAAATAAGGAGAGAGAGAA+AGG 0.446065 2:-32737915 MsaT008335.1:CDS
CTAATAAGCTCCAGAGATGC+TGG 0.447893 2:+32738077 None:intergenic
ATCCTCTTCAATCTTCTCCT+CGG 0.449369 2:+32738205 None:intergenic
GAGGGAGTCAGCAGTGGGAT+TGG 0.449422 2:+32738497 None:intergenic
CCATACCACTTCTGGGAGTA+AGG 0.450702 2:+32738453 None:intergenic
AAGAGAGAATTGAAGGCTGC+TGG 0.451049 2:-32739157 MsaT008335.1:CDS
TCCTGATAATCTTCTATGAC+AGG 0.451422 2:+32737861 None:intergenic
TCATACTCATCCATATCAAC+AGG 0.454291 2:+32739298 None:intergenic
ACCCACAATGGTATCACTAA+TGG 0.455710 2:+32739471 None:intergenic
TCCCCTGGACGCAATTTCCT+AGG 0.458724 2:+32739361 None:intergenic
AAAGGAGTCAAGGCTTGGTT+GGG 0.461730 2:+32738689 None:intergenic
TGCAGAGAGGCAAGATGCCC+CGG 0.470266 2:-32738915 MsaT008335.1:CDS
GAGTCTCAGACACCACTGTT+AGG 0.471291 2:-32738590 MsaT008335.1:CDS
CTGAAAATTTGGCCAGGCTG+AGG 0.471566 2:-32738613 MsaT008335.1:CDS
TTCTTAAAGGAGTCAAGGCT+TGG 0.471836 2:+32738684 None:intergenic
AATCTGAAGGATGCAACTCC+AGG 0.473048 2:+32738558 None:intergenic
TGGCATGCAGAATAGCAGCC+GGG 0.473211 2:+32738897 None:intergenic
TTGATATAAGGCAGCGGAGG+AGG 0.474103 2:-32739132 MsaT008335.1:CDS
TGATGTTGCTGATGAGGATA+GGG 0.479449 2:-32739044 MsaT008335.1:CDS
AATTTCATCCAATTCTTGAT+CGG 0.479560 2:+32738808 None:intergenic
GCGACCCGATCCTGTTGATA+TGG 0.481942 2:-32739308 MsaT008335.1:CDS
CCAGTATCTTCGTGAGGCAA+TGG 0.484875 2:-32737129 MsaT008335.1:CDS
TGTCTGAGACTCCCTCAGCC+TGG 0.484905 2:+32738601 None:intergenic
AACGATCAAAAGTCATTCAA+AGG 0.485643 2:-32738118 MsaT008335.1:CDS
TGCTTTCTGAAGCATGTGCT+CGG 0.486670 2:-32739267 MsaT008335.1:CDS
TCCTCTTCAATCTTCTCCTC+GGG 0.488427 2:+32738206 None:intergenic
GAGTAAGGCCTGCACTTCCA+GGG 0.490905 2:+32738468 None:intergenic
CTCCTTTCTTATCATCTCGT+CGG 0.493278 2:+32737989 None:intergenic
TCATTCAAAGGGAACTTCCT+AGG 0.493709 2:-32738106 MsaT008335.1:CDS
TGCACTTCCAGGGGTTGCAG+AGG 0.493979 2:+32738478 None:intergenic
ACGAGTAGCACTGCTGCCTT+CGG 0.495690 2:+32738739 None:intergenic
CTGACTCCCTCTGCAACCCC+TGG 0.496434 2:-32738485 MsaT008335.1:CDS
TCTTCTATGACAGGGATAGC+AGG 0.496725 2:+32737870 None:intergenic
TTCTGAAGCATGTGCTCGGT+TGG 0.497509 2:-32739263 MsaT008335.1:CDS
GTCTCCGTCAGCTCTTATCA+AGG 0.499862 2:+32738049 None:intergenic
TTGTCATGCTCCAGTAAGGC+TGG 0.499885 2:+32737963 None:intergenic
GTGAGAAGTAAGCTGGATGA+TGG 0.502979 2:-32736954 MsaT008335.1:CDS
CTCATCCATATCAACAGGAT+CGG 0.503902 2:+32739303 None:intergenic
AGAAGAAGAGGCACGGCAGA+AGG 0.504416 2:-32738153 MsaT008335.1:CDS
AAGTAAGCTGGATGATGGGA+AGG 0.506469 2:-32736949 MsaT008335.1:CDS
ATAGCATCTCCTTTACTAGC+TGG 0.506651 2:+32738647 None:intergenic
AGAGGATATGTCTGATAGAA+TGG 0.507303 2:-32738189 MsaT008335.1:CDS
AGCTCCAGAGATGCTGGAGG+AGG 0.508047 2:+32738083 None:intergenic
GATGATTCGACTTGAGAAGA+AGG 0.511403 2:-32736922 MsaT008335.1:CDS
TGATAGAATGGCTAGAGAAA+AGG 0.511992 2:-32738177 MsaT008335.1:CDS
ATTGCTGCCCACAAGGTCAC+TGG 0.512710 2:+32738769 None:intergenic
TTGGCATGCAGAATAGCAGC+CGG 0.514383 2:+32738896 None:intergenic
TTTGAGAAAAGGCCTCCTTT+GGG 0.515050 2:-32739073 MsaT008335.1:CDS
CAGAGAACACCAGCTAGTAA+AGG 0.515217 2:-32738656 MsaT008335.1:CDS
GTTGCAGAGGGAGTCAGCAG+TGG 0.516056 2:+32738491 None:intergenic
TGCAGATTTGCAAACGAGTA+GGG 0.518174 2:+32739615 None:intergenic
CATTGCCTCACGAAGATACT+GGG 0.519621 2:+32737130 None:intergenic
TGGGTGTCTGTGGATAATTA+GGG 0.519886 2:+32738708 None:intergenic
TTGATGTTGCTGATGAGGAT+AGG 0.524642 2:-32739045 MsaT008335.1:CDS
TCGGCTTGCTCAATAGGGGT+TGG 0.524733 2:+32738008 None:intergenic
TGAAAATTTGGCCAGGCTGA+GGG 0.525084 2:-32738612 MsaT008335.1:CDS
GACCCTAGGAAATTGCGTCC+AGG 0.525314 2:-32739364 MsaT008335.1:CDS
CTGCAGATTTGCAAACGAGT+AGG 0.526600 2:+32739614 None:intergenic
AGCATTGTCATGCTCCAGTA+AGG 0.527412 2:+32737959 None:intergenic
ACCATTAGTGATACCATTGT+GGG 0.527710 2:-32739472 MsaT008335.1:CDS
CAAGTTCTTCTATAGTAGTA+GGG 0.528361 2:+32739002 None:intergenic
TAAAGGAGTCAAGGCTTGGT+TGG 0.528607 2:+32738688 None:intergenic
GAAGTGGTATGGCGCTGGCA+AGG 0.528663 2:-32738442 MsaT008335.1:CDS
TGAGAATGTGAGAAGTAAGC+TGG 0.529343 2:-32736961 MsaT008335.1:CDS
GCTTGCTCAATAGGGGTTGG+TGG 0.531592 2:+32738011 None:intergenic
CACTCAGTCTTCTTAATGGA+AGG 0.532258 2:+32739556 None:intergenic
GCACTTCCAGGGGTTGCAGA+GGG 0.532965 2:+32738479 None:intergenic
TCATCCATATCAACAGGATC+GGG 0.534614 2:+32739304 None:intergenic
ACCCTAGGAAATTGCGTCCA+GGG 0.536757 2:-32739363 MsaT008335.1:CDS
AAGTGGTATGGCGCTGGCAA+GGG 0.537683 2:-32738441 MsaT008335.1:CDS
TCCCAGAAGTGGTATGGCGC+TGG 0.538783 2:-32738447 MsaT008335.1:CDS
ACTGTTAGGAGGAGAGAACC+TGG 0.540907 2:-32738576 MsaT008335.1:CDS
GATCCAAACGAGCAGCAAAT+GGG 0.542547 2:-32737893 MsaT008335.1:CDS
AGAGAGAATTGAAGGCTGCT+GGG 0.549517 2:-32739156 MsaT008335.1:CDS
TGATCGGAAATCTGAGGTGG+TGG 0.551298 2:+32738824 None:intergenic
CCTTACTCCCAGAAGTGGTA+TGG 0.551739 2:-32738453 MsaT008335.1:CDS
AGCCGACGAGATGATAAGAA+AGG 0.552168 2:-32737991 MsaT008335.1:CDS
GAAAAGAAGGATTGATGTTG+AGG 0.552508 2:-32738975 MsaT008335.1:CDS
GGTTATGCCAGTGACCTTGT+GGG 0.556103 2:-32738776 MsaT008335.1:CDS
TGAGAAAGCAAGACGTTGCA+AGG 0.556741 2:-32738946 MsaT008335.1:CDS
GGGTTATGCCAGTGACCTTG+TGG 0.560009 2:-32738777 MsaT008335.1:CDS
GGATTCGGATCAATCTCCCC+TGG 0.561665 2:+32739346 None:intergenic
CCCCTGGACGCAATTTCCTA+GGG 0.562986 2:+32739362 None:intergenic
AGAAACAGCTTGAGGAAGCA+AGG 0.565360 2:-32739201 MsaT008335.1:CDS
TCAACAGGATCGGGTCGCGC+AGG 0.566355 2:+32739313 None:intergenic
ACGATCAAAAGTCATTCAAA+GGG 0.567785 2:-32738117 MsaT008335.1:CDS
AGGAGTTCTTAAAGGAGTCA+AGG 0.570143 2:+32738679 None:intergenic
TTGCAGAGGGAGTCAGCAGT+GGG 0.571318 2:+32738492 None:intergenic
ACTACTATAGAAGAACTTGA+GGG 0.571318 2:-32738998 MsaT008335.1:CDS
TAAAGGAGATGCTATCATGA+TGG 0.573963 2:-32738639 MsaT008335.1:CDS
CTGAATGACCCAGAAACAGT+TGG 0.574442 2:-32738869 MsaT008335.1:CDS
TTACTAGCTGGTGTTCTCTG+AGG 0.578802 2:+32738659 None:intergenic
TAGAGAAAAGGCAGAAGAAG+AGG 0.579560 2:-32738165 MsaT008335.1:CDS
ATGAACCTGGTGATGACCCT+AGG 0.580871 2:-32739378 MsaT008335.1:CDS
TTATTTGCTATCTCCTCAAG+CGG 0.583374 2:+32737930 None:intergenic
GTCACTGGCATAACCCAACT+TGG 0.585958 2:+32738784 None:intergenic
AAGGTTACATTACTCACGCA+AGG 0.586053 2:-32736903 MsaT008335.1:CDS
AGTAAGGCCTGCACTTCCAG+GGG 0.586407 2:+32738469 None:intergenic
GTAAAGCTCGTTGGTATGAG+TGG 0.588526 2:-32739585 MsaT008335.1:CDS
AACTCCTTGATAAGAGCTGA+CGG 0.589304 2:-32738053 MsaT008335.1:CDS
AAGGCTTGGTTGGGTGTCTG+TGG 0.589342 2:+32738698 None:intergenic
CCTGATAATCTTCTATGACA+GGG 0.589597 2:+32737862 None:intergenic
GACTGAGTGGACTCGAGAAG+AGG 0.592125 2:-32739542 MsaT008335.1:CDS
TGCTTCAACAAAATCCTCGA+GGG 0.592155 2:+32737091 None:intergenic
CCTTAACACAAGTGGCGTCA+AGG 0.592424 2:+32739410 None:intergenic
GGTATGGCGCTGGCAAGGGA+TGG 0.593619 2:-32738437 MsaT008335.1:CDS
CTCCTTCGCAGTGTCTTGAA+CGG 0.594673 2:-32739444 MsaT008335.1:CDS
TGCTGATGAGGATAGGGAGG+TGG 0.595277 2:-32739038 MsaT008335.1:CDS
CAGAACGGTGTAAAGCTCGT+TGG 0.595953 2:-32739594 MsaT008335.1:CDS
AGTGTTGCCAAAATGAGCAT+AGG 0.596521 2:-32739718 None:intergenic
AACCATTAGTGATACCATTG+TGG 0.597390 2:-32739473 MsaT008335.1:CDS
TGAGAAGTAAGCTGGATGAT+GGG 0.598085 2:-32736953 MsaT008335.1:CDS
TACTACTATAGAAGAACTTG+AGG 0.598439 2:-32738999 MsaT008335.1:CDS
AAAAGGCAGAAGAAGAGGCA+CGG 0.599691 2:-32738160 MsaT008335.1:CDS
TGAGTTCATTGCTGCCCACA+AGG 0.602663 2:+32738762 None:intergenic
CTAATGGTTCTCCACTGAGT+AGG 0.603402 2:+32739487 None:intergenic
TGGAAGCTGAAAATTTGGCC+AGG 0.607474 2:-32738619 MsaT008335.1:CDS
GCTCGGTTGGCTAACACGAA+AGG 0.608258 2:-32739250 MsaT008335.1:CDS
TCTCAGACACCACTGTTAGG+AGG 0.609284 2:-32738587 MsaT008335.1:CDS
AGCGGAGGAGGAAAAGGAGA+GGG 0.609580 2:-32739120 MsaT008335.1:CDS
CAATGCTAAGTATCCGCTTG+AGG 0.613956 2:-32737943 MsaT008335.1:CDS
TACGAAGGGAACTCCTCTCA+GGG 0.614373 2:-32738396 MsaT008335.1:CDS
AATTTCCTAGGGTCATCACC+AGG 0.619740 2:+32739373 None:intergenic
CAGCGGAGGAGGAAAAGGAG+AGG 0.619802 2:-32739121 MsaT008335.1:CDS
CTGGGATTGATATAAGGCAG+CGG 0.621106 2:-32739138 MsaT008335.1:CDS
GTTATCGTCCTTAACACAAG+TGG 0.622208 2:+32739402 None:intergenic
GCAGGCCTTACTCCCAGAAG+TGG 0.624004 2:-32738458 MsaT008335.1:CDS
CGAAGGAGTACGACCCACAA+TGG 0.624367 2:+32739459 None:intergenic
CCCTAGGAAATTGCGTCCAG+GGG 0.631019 2:-32739362 MsaT008335.1:CDS
CTCGTCGGCTTGCTCAATAG+GGG 0.633774 2:+32738004 None:intergenic
TTTGATACCCGTAACACACA+TGG 0.638610 2:-32737038 MsaT008335.1:CDS
GGCATGCAGAATAGCAGCCG+GGG 0.639810 2:+32738898 None:intergenic
AAGGACGATAACTATGAACC+TGG 0.641325 2:-32739391 MsaT008335.1:CDS
TGTTGCTGATGAGGATAGGG+AGG 0.645550 2:-32739041 MsaT008335.1:CDS
AGGTTCTCTCCTCCTAACAG+TGG 0.645695 2:+32738578 None:intergenic
GCAACATCAAAATAACCCAA+AGG 0.645993 2:+32739058 None:intergenic
AGAAGATGCTGAAGAGCCCG+AGG 0.646686 2:-32738222 MsaT008335.1:CDS
CTACTATAGAAGAACTTGAG+GGG 0.654103 2:-32738997 MsaT008335.1:CDS
GCCCGAGGAGAAGATTGAAG+AGG 0.654306 2:-32738207 MsaT008335.1:CDS
AATTCTTGATCGGAAATCTG+AGG 0.665249 2:+32738818 None:intergenic
AGCTAGGGAGAAACAGCTTG+AGG 0.665565 2:-32739209 MsaT008335.1:CDS
TACCGTTCAAGACACTGCGA+AGG 0.666095 2:+32739442 None:intergenic
AGGTTACATTACTCACGCAA+GGG 0.667391 2:-32736902 MsaT008335.1:CDS
GGCAGCAATGAACTCACCGA+AGG 0.667861 2:-32738755 MsaT008335.1:CDS
CTAAGCTTACGCCTACTCAG+TGG 0.671805 2:-32739498 MsaT008335.1:CDS
TCGTTTGCAAATCTGCAGAA+CGG 0.675444 2:-32739609 MsaT008335.1:CDS
TCTTGATCGGAAATCTGAGG+TGG 0.678038 2:+32738821 None:intergenic
ACATCAAAATAACCCAAAGG+AGG 0.679868 2:+32739061 None:intergenic
CTTCCATTAAGAAGACTGAG+TGG 0.680364 2:-32739555 MsaT008335.1:CDS
TGAAGCCCAGTATCTTCGTG+AGG 0.688109 2:-32737135 MsaT008335.1:CDS
GTGCTTCAACAAAATCCTCG+AGG 0.688213 2:+32737090 None:intergenic
ATATGTAGCTCATCCCTGAG+AGG 0.688581 2:+32738383 None:intergenic
AGTATCTTCGTGAGGCAATG+GGG 0.691770 2:-32737127 MsaT008335.1:CDS
GGATTGATATAAGGCAGCGG+AGG 0.699913 2:-32739135 MsaT008335.1:CDS
ATAAGCTCCAGAGATGCTGG+AGG 0.701115 2:+32738080 None:intergenic
AACAGCTTGAGGAAGCAAGG+AGG 0.704973 2:-32739198 MsaT008335.1:CDS
TGATATGGATGAGTATGAGA+AGG 0.714958 2:-32739293 MsaT008335.1:CDS
TGTCATAGAAGATTATCAGG+AGG 0.727087 2:-32737859 MsaT008335.1:CDS
GCATGAAAATGATTCCCTCG+AGG 0.731393 2:-32737105 MsaT008335.1:CDS
CAAGGAATAAAATTGCAGAG+AGG 0.745082 2:-32738928 MsaT008335.1:CDS
AGGTGTTTGGACAAACACCG+AGG 0.789261 2:-32739698 MsaT008335.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 32736881 32739728 32736881 ID=MsaG008335
Chr2 mRNA 32736881 32739728 32736881 ID=MsaT008335.1;Parent=MsaG008335
Chr2 exon 32736881 32737177 32736881 ID=MsaT008335.1.exon2;Parent=MsaT008335.1
Chr2 CDS 32736881 32737177 32736881 ID=cds.MsaT008335.1;Parent=MsaT008335.1
Chr2 exon 32737845 32739728 32737845 ID=MsaT008335.1.exon1;Parent=MsaT008335.1
Chr2 CDS 32737845 32739728 32737845 ID=cds.MsaT008335.1;Parent=MsaT008335.1
Gene Sequence

>MsaT008335.1

ATGAGCATAGGTGTTTGGACAAACACCGAGGATGAAATCCTGAAAGCTGCTGTTATGAAATATGGTAAAAATCAGTGGGCTCATATCTCGTCCCTACTCGTTTGCAAATCTGCAGAACGGTGTAAAGCTCGTTGGTATGAGTGGTTAGATCCTTCCATTAAGAAGACTGAGTGGACTCGAGAAGAGGATGAGAAGCTACTTCATCTTGCTAAGCTTACGCCTACTCAGTGGAGAACCATTAGTGATACCATTGTGGGTCGTACTCCTTCGCAGTGTCTTGAACGGTATGAGAAACTCCTTGACGCCACTTGTGTTAAGGACGATAACTATGAACCTGGTGATGACCCTAGGAAATTGCGTCCAGGGGAGATTGATCCGAATCCTGAATCGAAGCCTGCGCGACCCGATCCTGTTGATATGGATGAGTATGAGAAGGAGATGCTTTCTGAAGCATGTGCTCGGTTGGCTAACACGAAAGGTAAAAAAGCAAAAAGGAAAGCTAGGGAGAAACAGCTTGAGGAAGCAAGGAGGCTTGCTTCTTTACAGAAAAAGAGAGAATTGAAGGCTGCTGGGATTGATATAAGGCAGCGGAGGAGGAAAAGGAGAGGGATTGATTATAATGCAGAAATTCCGTTTGAGAAAAGGCCTCCTTTGGGTTATTTTGATGTTGCTGATGAGGATAGGGAGGTGGAGCAGCATGCATTCCCTACTACTATAGAAGAACTTGAGGGGAAAAGAAGGATTGATGTTGAGGCTCAGTTGAGAAAGCAAGACGTTGCAAGGAATAAAATTGCAGAGAGGCAAGATGCCCCGGCTGCTATTCTGCATGCCAAGAAGCTGAATGACCCAGAAACAGTTGGAAAGAGATCAAAACTGATGCTTCCACCACCTCAGATTTCCGATCAAGAATTGGATGAAATTGCCAAGTTGGGTTATGCCAGTGACCTTGTGGGCAGCAATGAACTCACCGAAGGCAGCAGTGCTACTCGTGCTCTTCTCCCTAATTATCCACAGACACCCAACCAAGCCTTGACTCCTTTAAGAACTCCTCAGAGAACACCAGCTAGTAAAGGAGATGCTATCATGATGGAAGCTGAAAATTTGGCCAGGCTGAGGGAGTCTCAGACACCACTGTTAGGAGGAGAGAACCTGGAGTTGCATCCTTCAGATTTTTCTGGGGTCACTCCTAAGAAAAAGGAAATTCACATTCCAATCCCACTGCTGACTCCCTCTGCAACCCCTGGAAGTGCAGGCCTTACTCCCAGAAGTGGTATGGCGCTGGCAAGGGATGGTTTTTCTTTTGGCATGACTACGAAGGGAACTCCTCTCAGGGATGAGCTACATATCAATGAAGATATGGAGATGCGTGATAGTGCTAGACTCGAGCTACAGAGACAAGCTGATATGAAAAAAAGTCTGCGCTCTGGTTTAAGCAGCCTTCCACAGCCTAAAAATGAGTACCAGATAGTGATGCAACCAATTCAAGAAGATGCTGAAGAGCCCGAGGAGAAGATTGAAGAGGATATGTCTGATAGAATGGCTAGAGAAAAGGCAGAAGAAGAGGCACGGCAGAAGGTGTTACTTAGAAAACGATCAAAAGTCATTCAAAGGGAACTTCCTAGGCCTCCTCCAGCATCTCTGGAGCTTATTAGGAACTCCTTGATAAGAGCTGACGGAGACAAAAGTTCATTTGTTCCACCAACCCCTATTGAGCAAGCCGACGAGATGATAAGAAAGGAGCTTCCAGCCTTACTGGAGCATGACAATGCTAAGTATCCGCTTGAGGAGATAGCAAATAAGGAGAGAGAGAAAGGATCCAAACGAGCAGCAAATGGGCCTGCTATCCCTGTCATAGAAGATTATCAGGAGGACGAGATGAAATCTGCTGATCAGTTGATAAAAGATGAAGCCCAGTATCTTCGTGAGGCAATGGGGCATGAAAATGATTCCCTCGAGGATTTTGTTGAAGCACATACAACATGCATTAATGATTTAATGTACTTTGATACCCGTAACACACATGGTCTATCAAGTGTTGCTGGAAACGCAGAGAAGCTAGCAGCTTTGCAGAATGAATTTGAGAATGTGAGAAGTAAGCTGGATGATGGGAAGGAGAAGATGATTCGACTTGAGAAGAAGGTTACATTACTCACGCAAGGGTACGAGTTATGCTACTGCTAA
Protein sequence

>MsaT008335.1

MSIGVWTNTEDEILKAAVMKYGKNQWAHISSLLVCKSAERCKARWYEWLDPSIKKTEWTREEDEKLLHLAKLTPTQWRTISDTIVGRTPSQCLERYEKLLDATCVKDDNYEPGDDPRKLRPGEIDPNPESKPARPDPVDMDEYEKEMLSEACARLANTKGKKAKRKAREKQLEEARRLASLQKKRELKAAGIDIRQRRRKRRGIDYNAEIPFEKRPPLGYFDVADEDREVEQHAFPTTIEELEGKRRIDVEAQLRKQDVARNKIAERQDAPAAILHAKKLNDPETVGKRSKLMLPPPQISDQELDEIAKLGYASDLVGSNELTEGSSATRALLPNYPQTPNQALTPLRTPQRTPASKGDAIMMEAENLARLRESQTPLLGGENLELHPSDFSGVTPKKKEIHIPIPLLTPSATPGSAGLTPRSGMALARDGFSFGMTTKGTPLRDELHINEDMEMRDSARLELQRQADMKKSLRSGLSSLPQPKNEYQIVMQPIQEDAEEPEEKIEEDMSDRMAREKAEEEARQKVLLRKRSKVIQRELPRPPPASLELIRNSLIRADGDKSSFVPPTPIEQADEMIRKELPALLEHDNAKYPLEEIANKEREKGSKRAANGPAIPVIEDYQEDEMKSADQLIKDEAQYLREAMGHENDSLEDFVEAHTTCINDLMYFDTRNTHGLSSVAGNAEKLAALQNEFENVRSKLDDGKEKMIRLEKKVTLLTQGYELCYC*