AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008359

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008359 MtrunA17_Chr2g0302091 77.540 187 35 2 1 187 10 189 2.56e-94 273
MsaG008359 MtrunA17_Chr3g0093131 63.636 165 56 4 26 188 18 180 1.30e-59 184
MsaG008359 MtrunA17_Chr8g0345001 49.438 178 83 5 15 188 3 177 3.50e-45 147
MsaG008359 MtrunA17_Chr4g0018951 33.088 136 77 3 47 181 31 153 3.35e-17 75.1
MsaG008359 MtrunA17_Chr2g0333981 36.196 163 90 7 27 181 5 161 3.46e-17 75.1
MsaG008359 MtrunA17_Chr8g0379021 35.849 159 91 7 27 181 10 161 1.80e-15 70.9
MsaG008359 MtrunA17_Chr8g0355091 33.918 171 87 8 15 175 2 156 7.04e-14 67.4
MsaG008359 MtrunA17_Chr5g0422421 33.121 157 95 7 28 178 7 159 3.29e-13 65.9
MsaG008359 MtrunA17_Chr1g0206991 34.568 162 86 8 28 178 7 159 4.04e-13 64.7
MsaG008359 MtrunA17_Chr2g0292791 33.908 174 89 8 15 178 2 159 6.73e-13 65.1
MsaG008359 MtrunA17_Chr3g0099421 33.333 162 88 6 28 178 32 184 1.31e-12 64.3
MsaG008359 MtrunA17_Chr1g0206701 33.333 156 90 7 28 175 7 156 3.08e-12 63.2
MsaG008359 MtrunA17_Chr5g0422941 29.167 168 94 7 20 175 2 156 1.09e-11 61.6
MsaG008359 MtrunA17_Chr1g0149391 31.447 159 95 7 28 178 7 159 1.28e-11 61.2
MsaG008359 MtrunA17_Chr3g0119901 27.607 163 97 6 29 181 11 162 2.85e-11 60.1
MsaG008359 MtrunA17_Chr5g0419601 29.487 156 91 7 29 175 11 156 8.70e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008359 AT2G24840.1 28.289 152 105 4 29 178 65 214 1.47e-13 67.4
MsaG008359 AT5G60440.1 31.875 160 91 7 29 178 9 160 2.54e-13 67.0

Find 26 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGACTTTAAGAATTCTT+TGG 0.239126 2:+33158676 MsaT008359.1:CDS
GTGCTTCATATTGAAGCCTT+AGG 0.338858 2:-33158526 None:intergenic
TAGAATTATTCCACCAATCA+TGG 0.397833 2:-33158628 None:intergenic
TGATGTTGAATGAAACAAAC+TGG 0.408161 2:+33158174 None:intergenic
TGCTTCATATTGAAGCCTTA+GGG 0.444644 2:-33158525 None:intergenic
GCATAGAACTTGTTCTTGTC+CGG 0.453036 2:-33158401 None:intergenic
GTGATGTCAATTTCCATGAT+TGG 0.461626 2:+33158615 MsaT008359.1:CDS
ACGATGAATGACAGCATCAC+AGG 0.499770 2:-33158435 None:intergenic
GACATATGATGGTCGACGAT+GGG 0.514442 2:+33158468 MsaT008359.1:CDS
ATGAAATTGCTGAAACCCTA+AGG 0.533334 2:+33158510 MsaT008359.1:CDS
ATCGTCGACCATCATATGTC+CGG 0.542227 2:-33158465 None:intergenic
GAGTTCTCAAGACGCAAACT+CGG 0.557665 2:+33158311 MsaT008359.1:CDS
GGACAACATGAATGCTGCTA+GGG 0.558097 2:+33158196 MsaT008359.1:CDS
TCTTGTCCGGCGATTTGACG+AGG 0.561634 2:-33158388 None:intergenic
GGACATATGATGGTCGACGA+TGG 0.562958 2:+33158467 MsaT008359.1:CDS
GGGACAACATGAATGCTGCT+AGG 0.564881 2:+33158195 MsaT008359.1:CDS
TGCACTCCTCGTCAAATCGC+CGG 0.565424 2:+33158382 MsaT008359.1:CDS
GCTTCTGAGAGAAAGAATGC+TGG 0.579951 2:+33158218 MsaT008359.1:CDS
AATTTACGTGCAGCAAGCGA+AGG 0.589056 2:+33158713 MsaT008359.1:CDS
CGATGAATGACAGCATCACA+GGG 0.591570 2:-33158434 None:intergenic
ATGTCAATTTCCATGATTGG+TGG 0.630551 2:+33158618 MsaT008359.1:CDS
AAGAACAAGTTCTATGCATG+TGG 0.639320 2:+33158407 MsaT008359.1:CDS
GAACAACACTGAACAACACA+CGG 0.651271 2:+33158240 MsaT008359.1:CDS
GAAAGCTGTCAAAGTCACCA+AGG 0.688984 2:+33158583 MsaT008359.1:CDS
GATGTTGAATGAAACAAACT+GGG 0.726719 2:+33158175 None:intergenic
GATGAATGACAGCATCACAG+GGG 0.800398 2:-33158433 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 33158176 33158742 33158176 ID=MsaG008359
Chr2 mRNA 33158176 33158742 33158176 ID=MsaT008359.1;Parent=MsaG008359
Chr2 exon 33158176 33158742 33158176 ID=MsaT008359.1.exon1;Parent=MsaT008359.1
Chr2 CDS 33158176 33158742 33158176 ID=cds.MsaT008359.1;Parent=MsaT008359.1
Gene Sequence

>MsaT008359.1

ATGTTGAATGAAACAAACTGGGACAACATGAATGCTGCTAGGGCTTCTGAGAGAAAGAATGCTGGAACAACACTGAACAACACACGGAAGAGAAAGATGCATGAAATCAAGAAAGAAGAAAAACTGAACGGTTCTGAGTTCTCAAGACGCAAACTCGGAATATTCAACAAAGCAATAGAACTGTCTATTCTATGCCAATCAAAAACTGCACTCCTCGTCAAATCGCCGGACAAGAACAAGTTCTATGCATGTGGCTACCCCTGTGATGCTGTCATTCATCGTTTTCTGACCGGACATATGATGGTCGACGATGGGAAAAAGAAGAAACAAGATGATGAAATTGCTGAAACCCTAAGGCTTCAATATGAAGCACTTCAAGAAAAACTTGAAGAAGAGGAGGATAATTTGAAAGCTGTCAAAGTCACCAAGGCACAAAAGAGTGATGTCAATTTCCATGATTGGTGGAATAATTCTATTGATGATATGAATTTAGCATCTCTTGAAGACTTTAAGAATTCTTTGGAGAGTTTTAAGGGTAATTTACGTGCAGCAAGCGAAGGAAAGTAA
Protein sequence

>MsaT008359.1

MLNETNWDNMNAARASERKNAGTTLNNTRKRKMHEIKKEEKLNGSEFSRRKLGIFNKAIELSILCQSKTALLVKSPDKNKFYACGYPCDAVIHRFLTGHMMVDDGKKKKQDDEIAETLRLQYEALQEKLEEEEDNLKAVKVTKAQKSDVNFHDWWNNSIDDMNLASLEDFKNSLESFKGNLRAASEGK*