AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008360 MtrunA17_Chr2g0302091 78.537 205 28 3 1 205 1 189 4.45e-103 295
MsaG008360 MtrunA17_Chr3g0093131 61.053 190 62 6 19 206 1 180 1.72e-63 195
MsaG008360 MtrunA17_Chr8g0345001 50.000 178 82 5 33 206 3 177 4.11e-46 150
MsaG008360 MtrunA17_Chr4g0018951 33.824 136 76 3 65 199 31 153 4.30e-17 75.5
MsaG008360 MtrunA17_Chr2g0333981 36.420 162 91 6 45 199 5 161 5.14e-17 75.1
MsaG008360 MtrunA17_Chr8g0379021 34.810 158 94 6 45 199 10 161 4.60e-14 67.4
MsaG008360 MtrunA17_Chr8g0355091 34.503 171 86 8 33 193 2 156 5.19e-14 68.6
MsaG008360 MtrunA17_Chr2g0292791 34.320 169 90 7 38 196 2 159 1.33e-13 67.4
MsaG008360 MtrunA17_Chr5g0422421 30.864 162 105 6 38 196 2 159 2.63e-13 66.6
MsaG008360 MtrunA17_Chr1g0206991 34.177 158 92 7 46 196 7 159 7.12e-13 64.3
MsaG008360 MtrunA17_Chr3g0099421 35.366 164 82 8 46 196 32 184 1.66e-12 64.7
MsaG008360 MtrunA17_Chr1g0206701 33.133 166 90 7 38 193 2 156 1.67e-12 64.3
MsaG008360 MtrunA17_Chr1g0149391 30.120 166 101 7 38 196 2 159 2.70e-12 63.5
MsaG008360 MtrunA17_Chr3g0119901 27.607 163 97 6 47 199 11 162 3.74e-11 60.1
MsaG008360 MtrunA17_Chr3g0089141 31.737 167 97 6 38 196 2 159 4.19e-11 60.1
MsaG008360 MtrunA17_Chr1g0206721 31.953 169 94 7 38 196 2 159 5.72e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008360 AT2G24840.1 28.289 152 105 4 47 196 65 214 2.32e-13 67.4
MsaG008360 AT5G60440.1 32.075 159 92 7 47 196 9 160 5.93e-13 66.6

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGGACTTTAAGAATTCTT+TGG 0.239126 2:+33166381 MsaT008360.1:CDS
GTGCTTCATATTGAAGCCTT+AGG 0.338858 2:-33166231 None:intergenic
TAGAATTATTCCACCAATCA+TGG 0.397833 2:-33166333 None:intergenic
TGATGTTGAATGTAACAAAC+TGG 0.415899 2:+33165852 MsaT008360.1:CDS
TGCTTCATATTGAAGCCTTA+GGG 0.444644 2:-33166230 None:intergenic
GCATAGAACTTGTTCTTGTC+CGG 0.453036 2:-33166106 None:intergenic
GTGATGTCAATTTCCATGAT+TGG 0.461626 2:+33166320 MsaT008360.1:CDS
GAATTCTTTGGAGAGTTTCA+AGG 0.479356 2:+33166393 MsaT008360.1:CDS
AATTCTTTGGAGAGTTTCAA+GGG 0.490979 2:+33166394 MsaT008360.1:CDS
ACGATGAATGACAGCATCAC+AGG 0.499770 2:-33166140 None:intergenic
ATCCTCGGCCATCATATGTC+CGG 0.508496 2:-33166170 None:intergenic
GACATATGATGGCCGAGGAT+GGG 0.528388 2:+33166173 MsaT008360.1:CDS
ATGAAATTGCTGAAACCCTA+AGG 0.533334 2:+33166215 MsaT008360.1:CDS
GAGTTCTCAAGACGCAAACT+CGG 0.557665 2:+33166016 MsaT008360.1:CDS
TGTCTGAAAAGAACACTGCT+AGG 0.558447 2:+33165900 MsaT008360.1:CDS
TCTTGTCCGGCGATTTGACG+AGG 0.561634 2:-33166093 None:intergenic
GGACATATGATGGCCGAGGA+TGG 0.572212 2:+33166172 MsaT008360.1:CDS
GGCACTCCTCGTCAAATCGC+CGG 0.575658 2:+33166087 MsaT008360.1:CDS
GCTTCTGAGAGAAAGAATGC+TGG 0.579951 2:+33165923 MsaT008360.1:CDS
GACCGGACATATGATGGCCG+AGG 0.586444 2:+33166168 MsaT008360.1:CDS
AATTTACGTGCAGCAAGCGA+AGG 0.589056 2:+33166418 MsaT008360.1:CDS
CGATGAATGACAGCATCACA+GGG 0.591570 2:-33166139 None:intergenic
TATGAATTTAGCATCTCTTG+AGG 0.622708 2:+33166363 MsaT008360.1:CDS
GTCTGAAAAGAACACTGCTA+GGG 0.626438 2:+33165901 MsaT008360.1:CDS
ATGTCAATTTCCATGATTGG+TGG 0.630551 2:+33166323 MsaT008360.1:CDS
AAGAACAAGTTCTATGCATG+TGG 0.639320 2:+33166112 MsaT008360.1:CDS
GAACAAAACTGAACAACACA+CGG 0.651271 2:+33165945 MsaT008360.1:CDS
GATGTTGAATGTAACAAACT+GGG 0.728152 2:+33165853 MsaT008360.1:CDS
GAAAGCTGTCAAAGTCACCG+AGG 0.773790 2:+33166288 MsaT008360.1:CDS
GATGAATGACAGCATCACAG+GGG 0.800398 2:-33166138 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 33165827 33166447 33165827 ID=MsaG008360
Chr2 mRNA 33165827 33166447 33165827 ID=MsaT008360.1;Parent=MsaG008360
Chr2 exon 33165827 33166447 33165827 ID=MsaT008360.1.exon1;Parent=MsaT008360.1
Chr2 CDS 33165827 33166447 33165827 ID=cds.MsaT008360.1;Parent=MsaT008360.1
Gene Sequence

>MsaT008360.1

ATGTTATCAGATAATTCATCATCAGTGATGTTGAATGTAACAAACTGGGACAACATGAATAAGAATTGTGAACTGTCTGAAAAGAACACTGCTAGGGCTTCTGAGAGAAAGAATGCTGGAACAAAACTGAACAACACACGGAAGAGAAAGATGCATGAAATCAAGAAAGAAGAAAAACTGAACGGTTCTGAGTTCTCAAGACGCAAACTCGGAATATTCAACAAAGCAATAGAGCTGTCAATTCTATGCCAATCAAAAACGGCACTCCTCGTCAAATCGCCGGACAAGAACAAGTTCTATGCATGTGGCTACCCCTGTGATGCTGTCATTCATCGTTTTCTGACCGGACATATGATGGCCGAGGATGGGAAAAAGAAGAAGCAAGATGATGAAATTGCTGAAACCCTAAGGCTTCAATATGAAGCACTTCAAGAAAAACTTGAAGAAGAGGATGATAATTTGAAAGCTGTCAAAGTCACCGAGGCACAAAAGAGTGATGTCAATTTCCATGATTGGTGGAATAATTCTATTGATGATATGAATTTAGCATCTCTTGAGGACTTTAAGAATTCTTTGGAGAGTTTCAAGGGTAATTTACGTGCAGCAAGCGAAGGAAAGTAG
Protein sequence

>MsaT008360.1

MLSDNSSSVMLNVTNWDNMNKNCELSEKNTARASERKNAGTKLNNTRKRKMHEIKKEEKLNGSEFSRRKLGIFNKAIELSILCQSKTALLVKSPDKNKFYACGYPCDAVIHRFLTGHMMAEDGKKKKQDDEIAETLRLQYEALQEKLEEEDDNLKAVKVTEAQKSDVNFHDWWNNSIDDMNLASLEDFKNSLESFKGNLRAASEGK*