AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008361

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008361 MtrunA17_Chr2g0302091 80.612 196 31 2 1 196 1 189 8.00e-103 295
MsaG008361 MtrunA17_Chr3g0093131 65.455 165 53 4 35 197 18 180 7.30e-61 188
MsaG008361 MtrunA17_Chr8g0345001 51.124 178 80 5 24 197 3 177 2.02e-47 153
MsaG008361 MtrunA17_Chr2g0333981 37.654 162 89 6 36 190 5 161 6.86e-18 77.4
MsaG008361 MtrunA17_Chr4g0018951 33.824 136 76 3 56 190 31 153 1.98e-17 75.9
MsaG008361 MtrunA17_Chr8g0379021 36.076 158 92 6 36 190 10 161 6.94e-15 69.3
MsaG008361 MtrunA17_Chr8g0355091 33.918 171 87 8 24 184 2 156 8.41e-15 70.5
MsaG008361 MtrunA17_Chr2g0292791 33.728 169 91 7 29 187 2 159 4.06e-14 68.6
MsaG008361 MtrunA17_Chr3g0099421 35.366 164 82 8 37 187 32 184 2.71e-13 66.6
MsaG008361 MtrunA17_Chr1g0206991 34.146 164 84 8 37 187 7 159 2.99e-13 65.1
MsaG008361 MtrunA17_Chr5g0422421 30.864 162 105 6 29 187 2 159 3.23e-13 65.9
MsaG008361 MtrunA17_Chr1g0206701 32.530 166 91 7 29 184 2 156 3.76e-13 65.9
MsaG008361 MtrunA17_Chr1g0149391 30.723 166 100 7 29 187 2 159 1.06e-12 64.7
MsaG008361 MtrunA17_Chr3g0119901 28.834 163 95 6 38 190 11 162 1.09e-12 63.9
MsaG008361 MtrunA17_Chr3g0089141 31.325 166 97 6 30 187 3 159 4.19e-12 62.8
MsaG008361 MtrunA17_Chr3g0085241 28.221 163 96 6 38 190 43 194 6.87e-12 62.4
MsaG008361 MtrunA17_Chr1g0206721 31.361 169 95 7 29 187 2 159 1.02e-11 62.0
MsaG008361 MtrunA17_Chr1g0208561 31.138 167 98 6 29 187 2 159 1.08e-11 61.6
MsaG008361 MtrunA17_Chr4g0071601 37.607 117 68 4 56 171 31 143 4.79e-11 59.3
MsaG008361 MtrunA17_Chr5g0419601 28.916 166 97 7 29 184 2 156 5.23e-11 60.1
MsaG008361 MtrunA17_Chr1g0208581 32.759 174 91 8 24 187 2 159 7.04e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008361 AT2G24840.1 28.947 152 104 4 38 187 65 214 8.85e-14 68.2
MsaG008361 AT5G60440.1 32.704 159 91 7 38 187 9 160 1.83e-13 67.8

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGGACTTTAAGAATTCTT+TGG 0.239126 2:+33170676 MsaT008361.1:CDS
GTGCTTCATATTGAAGCCTT+AGG 0.338858 2:-33170526 None:intergenic
TAGAATTATTCCACCAATCA+TGG 0.397833 2:-33170628 None:intergenic
TGATGTTGAATGAAACAAAC+TGG 0.408161 2:+33170174 MsaT008361.1:CDS
TGCTTCATATTGAAGCCTTA+GGG 0.444644 2:-33170525 None:intergenic
GCATAGAACTTGTTCTTGTC+CGG 0.453036 2:-33170401 None:intergenic
GTGATGTCAATTTCCATGAT+TGG 0.461626 2:+33170615 MsaT008361.1:CDS
GAATTCTTTGGAGAGTTTCA+AGG 0.479356 2:+33170688 MsaT008361.1:CDS
ACGTTGAATGACAGCATCAC+AGG 0.479438 2:-33170435 None:intergenic
AATTCTTTGGAGAGTTTCAA+GGG 0.490979 2:+33170689 MsaT008361.1:CDS
ATGAAATTTCTGAAACCCTA+AGG 0.503280 2:+33170510 MsaT008361.1:CDS
ATCCTCGACCATCATATGTC+CGG 0.509285 2:-33170465 None:intergenic
GGACATATGATGGTCGAGGA+TGG 0.543275 2:+33170467 MsaT008361.1:CDS
TGCAATCATCGTCAAATCGC+CGG 0.556447 2:+33170382 MsaT008361.1:CDS
GAGTTCTCAAGACGCAAACT+CGG 0.557665 2:+33170311 MsaT008361.1:CDS
GGACAACATGAATGCTGCTA+GGG 0.558097 2:+33170196 MsaT008361.1:CDS
GACATATGATGGTCGAGGAT+GGG 0.560153 2:+33170468 MsaT008361.1:CDS
GGGACAACATGAATGCTGCT+AGG 0.564881 2:+33170195 MsaT008361.1:CDS
GCTTCTGAGAGAAAGAATGC+TGG 0.579951 2:+33170218 MsaT008361.1:CDS
AATTTACGTGCAGCAAGCGA+AGG 0.589056 2:+33170713 MsaT008361.1:CDS
CGTTGAATGACAGCATCACA+GGG 0.594896 2:-33170434 None:intergenic
GACCGGACATATGATGGTCG+AGG 0.605126 2:+33170463 MsaT008361.1:CDS
TATGAATTTAGCATCTCTTG+AGG 0.622708 2:+33170658 MsaT008361.1:CDS
ATGTCAATTTCCATGATTGG+TGG 0.630551 2:+33170618 MsaT008361.1:CDS
AAGAACAAGTTCTATGCATG+TGG 0.639320 2:+33170407 MsaT008361.1:CDS
GAACAAAACTGAACAACACA+CGG 0.651271 2:+33170240 MsaT008361.1:CDS
GATGTTGAATGAAACAAACT+GGG 0.726719 2:+33170175 MsaT008361.1:CDS
GAAAGCTGTCAAAGTCACCG+AGG 0.773790 2:+33170583 MsaT008361.1:CDS
GTTGAATGACAGCATCACAG+GGG 0.800398 2:-33170433 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 33170149 33170742 33170149 ID=MsaG008361
Chr2 mRNA 33170149 33170742 33170149 ID=MsaT008361.1;Parent=MsaG008361
Chr2 exon 33170149 33170742 33170149 ID=MsaT008361.1.exon1;Parent=MsaT008361.1
Chr2 CDS 33170149 33170742 33170149 ID=cds.MsaT008361.1;Parent=MsaT008361.1
Gene Sequence

>MsaT008361.1

ATGTTATCAGATAATTCATCATCAGTGATGTTGAATGAAACAAACTGGGACAACATGAATGCTGCTAGGGCTTCTGAGAGAAAGAATGCTGGAACAAAACTGAACAACACACGGAAGAGAAAGATGCATGAAATCAAGAAAGAAGAAAAACTGAACGGTTCTGAGTTCTCAAGACGCAAACTCGGAATATTCAACAAAGCAATAGAGCTGTCTATTCTATGCCAATCAAAAACTGCAATCATCGTCAAATCGCCGGACAAGAACAAGTTCTATGCATGTGGCTACCCCTGTGATGCTGTCATTCAACGTTTTCTGACCGGACATATGATGGTCGAGGATGGGAAAAAGAAGAAACAAGATGATGAAATTTCTGAAACCCTAAGGCTTCAATATGAAGCACTTCAAGAAAAACTTGAAGAAGAGGATGATAATTTGAAAGCTGTCAAAGTCACCGAGGCACAAAAGAGTGATGTCAATTTCCATGATTGGTGGAATAATTCTATTGATGATATGAATTTAGCATCTCTTGAGGACTTTAAGAATTCTTTGGAGAGTTTCAAGGGTAATTTACGTGCAGCAAGCGAAGGAAAGTAG
Protein sequence

>MsaT008361.1

MLSDNSSSVMLNETNWDNMNAARASERKNAGTKLNNTRKRKMHEIKKEEKLNGSEFSRRKLGIFNKAIELSILCQSKTAIIVKSPDKNKFYACGYPCDAVIQRFLTGHMMVEDGKKKKQDDEISETLRLQYEALQEKLEEEDDNLKAVKVTEAQKSDVNFHDWWNNSIDDMNLASLEDFKNSLESFKGNLRAASEGK*