AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008362

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008362 MtrunA17_Chr2g0302091 78.049 205 29 3 1 205 1 189 2.80e-102 293
MsaG008362 MtrunA17_Chr3g0093131 61.053 190 62 6 19 206 1 180 1.87e-63 195
MsaG008362 MtrunA17_Chr8g0345001 49.438 178 83 5 33 206 3 177 2.69e-45 148
MsaG008362 MtrunA17_Chr4g0018951 33.824 136 76 3 65 199 31 153 4.04e-17 75.5
MsaG008362 MtrunA17_Chr2g0333981 36.420 162 91 6 45 199 5 161 5.14e-17 75.1
MsaG008362 MtrunA17_Chr8g0379021 34.810 158 94 6 45 199 10 161 4.37e-14 67.4
MsaG008362 MtrunA17_Chr8g0355091 34.503 171 86 8 33 193 2 156 5.80e-14 68.2
MsaG008362 MtrunA17_Chr5g0422421 33.121 157 95 7 46 196 7 159 4.96e-13 65.9
MsaG008362 MtrunA17_Chr2g0292791 34.483 174 88 8 33 196 2 159 5.65e-13 65.5
MsaG008362 MtrunA17_Chr1g0206991 34.177 158 92 7 46 196 7 159 6.69e-13 64.3
MsaG008362 MtrunA17_Chr3g0099421 35.366 164 82 8 46 196 32 184 1.66e-12 64.7
MsaG008362 MtrunA17_Chr1g0206701 34.783 161 81 8 46 193 7 156 3.94e-12 63.2
MsaG008362 MtrunA17_Chr1g0149391 31.677 161 92 8 46 196 7 159 6.90e-12 62.4
MsaG008362 MtrunA17_Chr3g0119901 27.607 163 97 6 47 199 11 162 3.74e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008362 AT2G24840.1 28.289 152 105 4 47 196 65 214 2.14e-13 67.4
MsaG008362 AT5G60440.1 32.075 159 92 7 47 196 9 160 5.22e-13 66.6

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGGACTTTAAGAATTCTT+TGG 0.239126 2:+33178369 MsaT008362.1:CDS
GTGCTTCATATTGAAGCCTT+AGG 0.338858 2:-33178219 None:intergenic
TAGAATTATTCCACCAATCA+TGG 0.397833 2:-33178321 None:intergenic
TGATGTTGAATGTAACAAAC+TGG 0.415899 2:+33177840 MsaT008362.1:CDS
TGCTTCATATTGAAGCCTTA+GGG 0.444644 2:-33178218 None:intergenic
GCATAGAACTTGTTCTTGTC+CGG 0.453036 2:-33178094 None:intergenic
GTGATGTCAATTTCCATGAT+TGG 0.461626 2:+33178308 MsaT008362.1:CDS
GAATTCTTTGGAGAGTTTCA+AGG 0.479356 2:+33178381 MsaT008362.1:CDS
AATTCTTTGGAGAGTTTCAA+GGG 0.490979 2:+33178382 MsaT008362.1:CDS
ACGATGAATGACAGCATCAC+AGG 0.499770 2:-33178128 None:intergenic
ATCCTCGGCCATCATATGTC+CGG 0.508496 2:-33178158 None:intergenic
GACATATGATGGCCGAGGAT+GGG 0.528388 2:+33178161 MsaT008362.1:CDS
ATGAAATTGCTGAAACCCTA+AGG 0.533334 2:+33178203 MsaT008362.1:CDS
GAGTTCTCAAGACGCAAACT+CGG 0.557665 2:+33178004 MsaT008362.1:CDS
TGTCTGAAAAGAACACTGCT+AGG 0.558447 2:+33177888 MsaT008362.1:CDS
TCTTGTCCGGCGATTTGACG+AGG 0.561634 2:-33178081 None:intergenic
GGACATATGATGGCCGAGGA+TGG 0.572212 2:+33178160 MsaT008362.1:CDS
GGCACTCCTCGTCAAATCGC+CGG 0.575658 2:+33178075 MsaT008362.1:CDS
GCTTCTGAGAGAAAGAATGC+TGG 0.579951 2:+33177911 MsaT008362.1:CDS
GAACAACACTGAACAACACT+CGG 0.583442 2:+33177933 MsaT008362.1:CDS
GACCGGACATATGATGGCCG+AGG 0.586444 2:+33178156 MsaT008362.1:CDS
AATTTACGTGCAGCAAGCGA+AGG 0.589056 2:+33178406 MsaT008362.1:CDS
CGATGAATGACAGCATCACA+GGG 0.591570 2:-33178127 None:intergenic
TATGAATTTAGCATCTCTTG+AGG 0.622708 2:+33178351 MsaT008362.1:CDS
GTCTGAAAAGAACACTGCTA+GGG 0.626438 2:+33177889 MsaT008362.1:CDS
ATGTCAATTTCCATGATTGG+TGG 0.630551 2:+33178311 MsaT008362.1:CDS
AAGAACAAGTTCTATGCATG+TGG 0.639320 2:+33178100 MsaT008362.1:CDS
GATGTTGAATGTAACAAACT+GGG 0.728152 2:+33177841 MsaT008362.1:CDS
GAAAGCTGTCAAAGTCACCG+AGG 0.773790 2:+33178276 MsaT008362.1:CDS
GATGAATGACAGCATCACAG+GGG 0.800398 2:-33178126 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 33177815 33178435 33177815 ID=MsaG008362
Chr2 mRNA 33177815 33178435 33177815 ID=MsaT008362.1;Parent=MsaG008362
Chr2 exon 33177815 33178435 33177815 ID=MsaT008362.1.exon1;Parent=MsaT008362.1
Chr2 CDS 33177815 33178435 33177815 ID=cds.MsaT008362.1;Parent=MsaT008362.1
Gene Sequence

>MsaT008362.1

ATGTTATCAGATAATTCATCATCAGTGATGTTGAATGTAACAAACTGGGACAACATGAATAAGAATTGTGAACTGTCTGAAAAGAACACTGCTAGGGCTTCTGAGAGAAAGAATGCTGGAACAACACTGAACAACACTCGGAAGAGAAAGATGCATGAAATCAAGAAAGAAGAAAAACTGAACGGTTCTGAGTTCTCAAGACGCAAACTCGGAATATTCAACAAAGCAATAGAGCTGTCAATTCTATGCCAATCAAAAACGGCACTCCTCGTCAAATCGCCGGACAAGAACAAGTTCTATGCATGTGGCTACCCCTGTGATGCTGTCATTCATCGTTTTCTGACCGGACATATGATGGCCGAGGATGGGAAAAAGAAGAAGCAAGATGATGAAATTGCTGAAACCCTAAGGCTTCAATATGAAGCACTTCAAGAAAAACTTGAAGAAGAGGATGATAATTTGAAAGCTGTCAAAGTCACCGAGGCACAAAAGAGTGATGTCAATTTCCATGATTGGTGGAATAATTCTATTGATGATATGAATTTAGCATCTCTTGAGGACTTTAAGAATTCTTTGGAGAGTTTCAAGGGTAATTTACGTGCAGCAAGCGAAGGAAAGTAG
Protein sequence

>MsaT008362.1

MLSDNSSSVMLNVTNWDNMNKNCELSEKNTARASERKNAGTTLNNTRKRKMHEIKKEEKLNGSEFSRRKLGIFNKAIELSILCQSKTALLVKSPDKNKFYACGYPCDAVIHRFLTGHMMAEDGKKKKQDDEIAETLRLQYEALQEKLEEEDDNLKAVKVTEAQKSDVNFHDWWNNSIDDMNLASLEDFKNSLESFKGNLRAASEGK*