AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008363


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008363 MtrunA17_Chr2g0302091 84.653 202 30 1 1 201 1 202 9.21e-118 332
MsaG008363 MtrunA17_Chr3g0093131 61.905 189 56 5 15 195 6 186 1.76e-65 200
MsaG008363 MtrunA17_Chr8g0345001 46.842 190 81 6 23 201 3 183 6.84e-43 142
MsaG008363 MtrunA17_Chr2g0333981 38.272 162 81 6 35 182 5 161 6.12e-19 80.1
MsaG008363 MtrunA17_Chr4g0018951 34.586 133 73 3 51 182 34 153 3.71e-17 75.5
MsaG008363 MtrunA17_Chr8g0379021 35.465 172 90 7 35 196 10 170 3.63e-15 70.1
MsaG008363 MtrunA17_Chr5g0422421 32.051 156 91 6 36 179 7 159 1.44e-12 64.3
MsaG008363 MtrunA17_Chr3g0099421 31.677 161 85 5 36 179 32 184 9.38e-12 62.4
MsaG008363 MtrunA17_Chr8g0355091 32.948 173 79 8 23 176 2 156 9.71e-12 62.0
MsaG008363 MtrunA17_Chr3g0119901 32.168 143 77 6 50 182 30 162 1.01e-11 61.6
MsaG008363 MtrunA17_Chr1g0206701 31.613 155 87 6 36 176 7 156 1.39e-11 61.6
MsaG008363 MtrunA17_Chr1g0206991 30.864 162 85 7 36 179 7 159 2.55e-11 60.1
MsaG008363 MtrunA17_Chr3g0085241 30.769 143 79 6 50 182 62 194 4.38e-11 60.5
MsaG008363 MtrunA17_Chr1g0149391 30.380 158 91 6 36 179 7 159 5.01e-11 60.1
MsaG008363 MtrunA17_Chr5g0422941 30.061 163 79 7 36 176 7 156 5.27e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008363 AT5G60440.1 30.818 159 97 7 51 201 29 182 7.99e-13 66.2
MsaG008363 AT2G24840.1 26.882 186 114 6 37 201 65 249 1.64e-12 64.7

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGTAGCAATGTTTATATT+TGG 0.182142 2:-33191004 None:intergenic
GTTTATATTTGGATGTAAAT+TGG 0.215750 2:-33190993 None:intergenic
TGAGGACTTTAAGAATTCTT+TGG 0.239126 2:+33190925 MsaT008363.1:CDS
GAATAATTCTATTGATGATA+TGG 0.255478 2:+33190889 MsaT008363.1:CDS
GTGCTTCATATTGAAGCCTT+AGG 0.338858 2:-33190775 None:intergenic
TAGAATTATTCCACCAATCA+TGG 0.397833 2:-33190877 None:intergenic
TGAAGAAGAGCAGGATACTT+TGG 0.413235 2:+33190811 MsaT008363.1:CDS
TGCTTCATATTGAAGCCTTA+GGG 0.444644 2:-33190774 None:intergenic
GCATAGAACTTGTTCTTGTC+CGG 0.453036 2:-33190650 None:intergenic
GAATTCTTTGGAGAGTTTCA+AGG 0.479356 2:+33190937 MsaT008363.1:CDS
TTCAGCAAGCGAAGCAGAGA+AGG 0.486404 2:+33190970 MsaT008363.1:CDS
AATTCTTTGGAGAGTTTCAA+GGG 0.490979 2:+33190938 MsaT008363.1:CDS
GATGCATGAAATCAAGAAAG+AGG 0.498636 2:+33190541 MsaT008363.1:CDS
ACGATGAATGACAGCATCAC+AGG 0.499770 2:-33190684 None:intergenic
ATCCTCGACCATCATATGTC+CGG 0.509285 2:-33190714 None:intergenic
ACATGAATAAGAAAATTGCT+AGG 0.527390 2:+33190465 MsaT008363.1:CDS
ATGAAATTGCTGAAACCCTA+AGG 0.533334 2:+33190759 MsaT008363.1:CDS
GGACATATGATGGTCGAGGA+TGG 0.543275 2:+33190716 MsaT008363.1:CDS
TGCAATCATCGTCAAATCGC+CGG 0.556447 2:+33190631 MsaT008363.1:CDS
GACATATGATGGTCGAGGAT+GGG 0.560153 2:+33190717 MsaT008363.1:CDS
CATGAATAAGAAAATTGCTA+GGG 0.586387 2:+33190466 MsaT008363.1:CDS
CGATGAATGACAGCATCACA+GGG 0.591570 2:-33190683 None:intergenic
GACCGGACATATGATGGTCG+AGG 0.605126 2:+33190712 MsaT008363.1:CDS
AAGAACAAGTTCTATGCATG+TGG 0.639320 2:+33190656 MsaT008363.1:CDS
TATGGATTTAGCATCTCTTG+AGG 0.650206 2:+33190907 MsaT008363.1:CDS
GAACAAAACTCAACAACACA+CGG 0.651271 2:+33190510 MsaT008363.1:CDS
GCTAGTGAGAGAAAGAACCC+TGG 0.692523 2:+33190488 MsaT008363.1:CDS
GGAAGCTGTCAAAGTCACTG+AGG 0.723299 2:+33190832 MsaT008363.1:CDS
GATGAATGACAGCATCACAG+GGG 0.800398 2:-33190682 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 33190422 33191027 33190422 ID=MsaG008363
Chr2 mRNA 33190422 33191027 33190422 ID=MsaT008363.1;Parent=MsaG008363
Chr2 exon 33190422 33191027 33190422 ID=MsaT008363.1.exon1;Parent=MsaT008363.1
Chr2 CDS 33190422 33191027 33190422 ID=cds.MsaT008363.1;Parent=MsaT008363.1
Gene Sequence

>MsaT008363.1

ATGTTATCAGATAATTCATCATCAGTGATGTTGTATGAAACAGACATGAATAAGAAAATTGCTAGGGCTAGTGAGAGAAAGAACCCTGGAACAAAACTCAACAACACACGGAAGAGAAAGATGCATGAAATCAAGAAAGAGGAAAAAGAACGCAGACTCGGAATTTTCAACAAAGCGATAGAGCTGTCTATTCTATGCCAATCAAAAACTGCAATCATCGTCAAATCGCCGGACAAGAACAAGTTCTATGCATGTGGCTACCCCTGTGATGCTGTCATTCATCGTTTTCTGACCGGACATATGATGGTCGAGGATGGGAAAAAGAAGAAACAAGATGATGAAATTGCTGAAACCCTAAGGCTTCAATATGAAGCACTTCAAGAAAAACTTGAAGAAGAGCAGGATACTTTGGAAGCTGTCAAAGTCACTGAGGCACAAAAGAGTGATTTTAATTTCCATGATTGGTGGAATAATTCTATTGATGATATGGATTTAGCATCTCTTGAGGACTTTAAGAATTCTTTGGAGAGTTTCAAGGGTAATTTACGTTCAGCAAGCGAAGCAGAGAAGGCCAATTTACATCCAAATATAAACATTGCTACTTAA

Protein sequence

>MsaT008363.1

MLSDNSSSVMLYETDMNKKIARASERKNPGTKLNNTRKRKMHEIKKEEKERRLGIFNKAIELSILCQSKTAIIVKSPDKNKFYACGYPCDAVIHRFLTGHMMVEDGKKKKQDDEIAETLRLQYEALQEKLEEEQDTLEAVKVTEAQKSDFNFHDWWNNSIDDMDLASLEDFKNSLESFKGNLRSASEAEKANLHPNINIAT*