Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG008381 | XP_039686054.1 | 87.903 | 124 | 15 | 0 | 1 | 124 | 122 | 245 | 7.18e-71 | 222 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG008381 | sp|Q99091|CPRF3_PETCR | 41.667 | 84 | 47 | 1 | 37 | 118 | 192 | 275 | 3.69e-15 | 72.8 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG008381 | G7IST9 | 87.903 | 124 | 15 | 0 | 1 | 124 | 158 | 281 | 8.59e-71 | 222 |
Gene ID | Type | Classification |
---|---|---|
MsaG008381 | TF | bZIP |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000135 | MsaG008381 | 0.826780 | 4.005677e-54 | 1.058585e-51 |
MsaG000325 | MsaG008381 | 0.834535 | 5.154670e-56 | 1.702820e-53 |
MsaG000408 | MsaG008381 | 0.819597 | 1.869517e-52 | 4.067870e-50 |
MsaG000613 | MsaG008381 | 0.827042 | 3.471308e-54 | 9.240910e-52 |
MsaG000648 | MsaG008381 | 0.822186 | 4.771610e-53 | 1.112085e-50 |
MsaG001133 | MsaG008381 | 0.803132 | 6.784426e-49 | 9.850197e-47 |
MsaG002481 | MsaG008381 | 0.802433 | 9.445656e-49 | 1.349544e-46 |
MsaG003804 | MsaG008381 | 0.800379 | 2.477493e-48 | 3.378521e-46 |
MsaG004280 | MsaG008381 | 0.810992 | 1.498957e-50 | 2.622444e-48 |
MsaG004654 | MsaG008381 | 0.800099 | 2.822481e-48 | 3.824685e-46 |
MsaG004887 | MsaG008381 | 0.800146 | 2.761307e-48 | 3.745747e-46 |
MsaG005728 | MsaG008381 | 0.824818 | 1.164452e-53 | 2.914647e-51 |
MsaG006240 | MsaG008381 | 0.802986 | 7.271806e-49 | 1.052220e-46 |
MsaG007734 | MsaG008381 | 0.817628 | 5.202559e-52 | 1.075493e-49 |
MsaG008381 | MsaG008511 | 0.808196 | 5.935796e-50 | 9.705406e-48 |
MsaG008381 | MsaG009117 | 0.831147 | 3.547465e-55 | 1.060970e-52 |
MsaG008381 | MsaG010637 | 0.818481 | 3.343610e-52 | 7.066180e-50 |
MsaG008381 | MsaG011481 | 0.853807 | 3.604613e-61 | 2.231688e-58 |
MsaG008381 | MsaG011812 | 0.812415 | 7.371147e-51 | 1.335547e-48 |
MsaG008381 | MsaG012046 | 0.848117 | 1.422462e-59 | 7.231814e-57 |
MsaG008381 | MsaG013974 | 0.800118 | 2.798551e-48 | 3.793800e-46 |
MsaG008381 | MsaG015829 | 0.804311 | 3.872182e-49 | 5.777514e-47 |
MsaG008381 | MsaG016695 | 0.823947 | 1.861619e-53 | 4.550386e-51 |
MsaG008381 | MsaG016974 | 0.802154 | 1.077436e-48 | 1.529536e-46 |
MsaG008381 | MsaG017183 | 0.808336 | 5.544151e-50 | 9.095375e-48 |
MsaG008381 | MsaG017219 | 0.818047 | 4.187390e-52 | 8.750747e-50 |
MsaG008381 | MsaG017290 | 0.830194 | 6.056822e-55 | 1.762635e-52 |
MsaG008381 | MsaG017483 | 0.816823 | 7.877522e-52 | 1.595099e-49 |
MsaG008381 | MsaG018082 | 0.807615 | 7.881010e-50 | 1.270678e-47 |
MsaG008381 | MsaG018598 | 0.837176 | 1.111467e-56 | 3.974913e-54 |
MsaG008381 | MsaG018641 | 0.805428 | 2.268999e-49 | 3.474190e-47 |
MsaG008381 | MsaG019183 | 0.855797 | 9.602578e-62 | 6.384972e-59 |
MsaG008381 | MsaG019188 | 0.842802 | 3.855478e-58 | 1.645052e-55 |
MsaG008381 | MsaG019806 | 0.808011 | 6.495977e-50 | 1.057438e-47 |
MsaG008381 | MsaG020732 | 0.806314 | 1.480283e-49 | 2.314447e-47 |
MsaG008381 | MsaG020716 | 0.819658 | 1.810412e-52 | 3.945612e-50 |
MsaG008381 | MsaG020834 | 0.815662 | 1.427668e-51 | 2.806517e-49 |
MsaG008381 | MsaG020981 | 0.826957 | 3.636023e-54 | 9.656816e-52 |
MsaG008381 | MsaG021395 | 0.830198 | 6.042381e-55 | 1.758675e-52 |
MsaG008381 | MsaG021650 | 0.813694 | 3.875943e-51 | 7.250703e-49 |
MsaG008381 | MsaG021720 | 0.802110 | 1.100086e-48 | 1.560091e-46 |
MsaG008381 | MsaG022325 | 0.800807 | 2.028404e-48 | 2.792981e-46 |
MsaG008381 | MsaG022627 | 0.820415 | 1.216963e-52 | 2.706205e-50 |
MsaG008381 | MsaG023268 | 0.819687 | 1.783529e-52 | 3.890016e-50 |
MsaG008381 | MsaG023796 | 0.833027 | 1.223284e-55 | 3.864589e-53 |
MsaG008381 | MsaG026961 | 0.831294 | 3.266086e-55 | 9.809444e-53 |
MsaG008381 | MsaG027505 | 0.808917 | 4.171613e-50 | 6.940344e-48 |
MsaG008381 | MsaG027557 | 0.836473 | 1.676917e-56 | 5.871030e-54 |
MsaG008381 | MsaG027590 | 0.815549 | 1.512922e-51 | 2.965494e-49 |
MsaG008381 | MsaG027725 | 0.819339 | 2.139402e-52 | 4.624240e-50 |
MsaG008381 | MsaG027845 | 0.820148 | 1.400126e-52 | 3.091636e-50 |
MsaG008381 | MsaG028628 | 0.805746 | 1.946448e-49 | 3.002658e-47 |
MsaG008381 | MsaG028802 | 0.824332 | 1.513250e-53 | 3.738012e-51 |
MsaG008381 | MsaG028844 | 0.812805 | 6.063693e-51 | 1.109366e-48 |
MsaG008381 | MsaG029035 | 0.819513 | 1.952578e-52 | 4.239498e-50 |
MsaG008381 | MsaG029063 | 0.827006 | 3.539247e-54 | 9.412308e-52 |
MsaG008381 | MsaG029147 | 0.803399 | 5.977870e-49 | 8.732707e-47 |
MsaG008381 | MsaG030196 | 0.826025 | 6.048648e-54 | 1.565352e-51 |
MsaG008381 | MsaG031494 | 0.822266 | 4.573469e-53 | 1.068199e-50 |
MsaG008381 | MsaG032491 | 0.817221 | 6.416885e-52 | 1.312800e-49 |
MsaG008381 | MsaG034853 | 0.809177 | 3.671916e-50 | 6.147186e-48 |
MsaG008381 | MsaG039573 | 0.838956 | 3.892872e-57 | 1.470876e-54 |
MsaG008381 | MsaG040027 | 0.800480 | 2.363683e-48 | 3.230661e-46 |
MsaG008381 | MsaG040728 | 0.826392 | 4.952260e-54 | 1.294743e-51 |
MsaG008381 | MsaG040850 | 0.834423 | 5.497352e-56 | 1.810018e-53 |
MsaG008381 | MsaG042102 | 0.826485 | 4.708348e-54 | 1.234171e-51 |
MsaG008381 | MsaG044546 | 0.803084 | 6.940595e-49 | 1.006575e-46 |
MsaG008381 | MsaG044855 | 0.803918 | 4.670614e-49 | 6.905500e-47 |
MsaG008381 | MsaG045510 | 0.813502 | 4.271343e-51 | 7.951803e-49 |
MsaG008381 | MsaG045822 | 0.817324 | 6.087452e-52 | 1.248659e-49 |
MsaG008381 | MsaG046054 | 0.858695 | 1.350776e-62 | 9.995467e-60 |
MsaG008381 | MsaG046540 | 0.810665 | 1.762908e-50 | 3.059679e-48 |
MsaG008381 | MsaG046660 | 0.843944 | 1.916964e-58 | 8.483985e-56 |
MsaG008381 | MsaG046780 | 0.800459 | 2.386453e-48 | 3.260286e-46 |
MsaG008381 | MsaG001966 | 0.814434 | 2.666860e-51 | 5.082403e-49 |
MsaG008381 | MsaG002578 | 0.803357 | 6.100044e-49 | 8.902495e-47 |
MsaG008381 | MsaG014406 | 0.827720 | 2.390630e-54 | 6.486444e-52 |
MsaG008381 | MsaG013859 | 0.816767 | 8.107145e-52 | 1.639267e-49 |
MsaG008381 | MsaG014017 | 0.803626 | 5.367049e-49 | 7.881487e-47 |
MsaG008381 | MsaG011821 | 0.803403 | 5.966463e-49 | 8.716931e-47 |
MsaG008381 | MsaG027840 | 0.813650 | 3.963811e-51 | 7.406759e-49 |
MsaG008381 | MsaG032991 | 0.820214 | 1.352424e-52 | 2.991565e-50 |
MsaG008381 | MsaG032664 | 0.824544 | 1.350117e-53 | 3.354260e-51 |
MsaG008381 | MsaG037725 | 0.802288 | 1.011492e-48 | 1.440385e-46 |
MsaG008381 | MsaG036408 | 0.820414 | 1.217696e-52 | 2.707736e-50 |
MsaG008381 | MsaG037728 | 0.802773 | 8.045382e-49 | 1.158416e-46 |
MsaG008381 | MsaG043116 | 0.802472 | 9.273122e-49 | 1.326083e-46 |
MsaG008381 | MsaG043656 | 0.815273 | 1.741169e-51 | 3.389208e-49 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG008381 | MtrunA17_Chr1g0162721 | 76.800 | 125 | 28 | 1 | 1 | 124 | 183 | 307 | 1.49e-58 | 182 |
MsaG008381 | MtrunA17_Chr2g0302481 | 54.839 | 124 | 30 | 2 | 1 | 124 | 91 | 188 | 3.19e-35 | 119 |
MsaG008381 | MtrunA17_Chr4g0065261 | 59.551 | 89 | 35 | 1 | 36 | 124 | 88 | 175 | 2.64e-26 | 96.7 |
MsaG008381 | MtrunA17_Chr8g0373571 | 43.182 | 88 | 50 | 0 | 31 | 118 | 252 | 339 | 1.46e-15 | 71.2 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG008381 | AT4G36730.2 | 40.964 | 83 | 47 | 1 | 38 | 118 | 217 | 299 | 2.69e-12 | 62.4 |
MsaG008381 | AT4G36730.1 | 40.964 | 83 | 47 | 1 | 38 | 118 | 219 | 301 | 2.99e-12 | 62.0 |
MsaG008381 | AT2G35530.1 | 39.437 | 71 | 43 | 0 | 36 | 106 | 302 | 372 | 2.18e-11 | 60.1 |
Find 18 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
ATCTGGGATGGCTCAGAATT+TGG | 0.139505 | 2:-33442358 | MsaT008381.1:CDS |
CACTCTTAAGGATGAAAATT+TGG | 0.215284 | 2:-33441861 | MsaT008381.1:CDS |
TCTGGGATGGCTCAGAATTT+GGG | 0.322428 | 2:-33442357 | MsaT008381.1:CDS |
GCTCAGCGAGCGTTTGTGTT+AGG | 0.327287 | 2:+33441837 | None:intergenic |
TTCTTTGTAGAAAATCCATC+TGG | 0.371434 | 2:-33442375 | MsaT008381.1:intron |
GAAGAGCTCGTTAAGGAGTA+TGG | 0.378867 | 2:-33441342 | MsaT008381.1:CDS |
TCAAAAGATAAACACTCTTA+AGG | 0.422261 | 2:-33441873 | MsaT008381.1:CDS |
AAGATCAAAGATAAAGAAAC+AGG | 0.437513 | 2:-33442073 | MsaT008381.1:intron |
AATAGATCCGCAATTGATTC+TGG | 0.439062 | 2:+33441318 | None:intergenic |
CAATGAAAACGATTCTATTG+AGG | 0.546332 | 2:-33441789 | MsaT008381.1:intron |
GGAACAGGAAGAGCTCGTTA+AGG | 0.581436 | 2:-33441349 | MsaT008381.1:intron |
AAATTCTGAGCCATCCCAGA+TGG | 0.581446 | 2:+33442360 | None:intergenic |
TCTAACATGATTATTAGTGA+TGG | 0.585817 | 2:-33442533 | None:intergenic |
CTGGGATGGCTCAGAATTTG+GGG | 0.588703 | 2:-33442356 | MsaT008381.1:CDS |
TGTAGAAAATCCATCTGGGA+TGG | 0.598950 | 2:-33442370 | MsaT008381.1:intron |
TCTTTGTAGAAAATCCATCT+GGG | 0.602431 | 2:-33442374 | MsaT008381.1:intron |
GAATTTGGGGACAGTCGTCA+AGG | 0.625862 | 2:-33442343 | MsaT008381.1:CDS |
GTATGGACCAGAATCAATTG+CGG | 0.711023 | 2:-33441325 | MsaT008381.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr2 | gene | 33441299 | 33442549 | 33441299 | ID=MsaG008381 |
Chr2 | mRNA | 33441299 | 33442549 | 33441299 | ID=MsaT008381.1;Parent=MsaG008381 |
Chr2 | exon | 33441299 | 33441364 | 33441299 | ID=MsaT008381.1.exon5;Parent=MsaT008381.1 |
Chr2 | CDS | 33441299 | 33441364 | 33441299 | ID=cds.MsaT008381.1;Parent=MsaT008381.1 |
Chr2 | exon | 33441790 | 33441915 | 33441790 | ID=MsaT008381.1.exon4;Parent=MsaT008381.1 |
Chr2 | CDS | 33441790 | 33441915 | 33441790 | ID=cds.MsaT008381.1;Parent=MsaT008381.1 |
Chr2 | exon | 33442074 | 33442151 | 33442074 | ID=MsaT008381.1.exon3;Parent=MsaT008381.1 |
Chr2 | CDS | 33442074 | 33442151 | 33442074 | ID=cds.MsaT008381.1;Parent=MsaT008381.1 |
Chr2 | exon | 33442299 | 33442387 | 33442299 | ID=MsaT008381.1.exon2;Parent=MsaT008381.1 |
Chr2 | CDS | 33442299 | 33442387 | 33442299 | ID=cds.MsaT008381.1;Parent=MsaT008381.1 |
Chr2 | exon | 33442534 | 33442549 | 33442534 | ID=MsaT008381.1.exon1;Parent=MsaT008381.1 |
Chr2 | CDS | 33442534 | 33442549 | 33442534 | ID=cds.MsaT008381.1;Parent=MsaT008381.1 |
Gene Sequence |
Protein sequence |