AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008385

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008385 MtrunA17_Chr1g0162721 60.518 309 66 8 22 276 1 307 1.65e-111 324
MsaG008385 MtrunA17_Chr2g0302481 59.906 212 60 1 65 276 2 188 2.26e-81 243
MsaG008385 MtrunA17_Chr2g0302501 63.793 116 36 2 15 124 13 128 1.37e-40 137
MsaG008385 MtrunA17_Chr4g0065261 62.921 89 32 1 188 276 88 175 2.79e-28 106
MsaG008385 MtrunA17_Chr8g0373571 46.250 80 43 0 191 270 260 339 4.44e-18 82.8
MsaG008385 MtrunA17_Chr5g0410531 39.583 96 56 2 169 263 286 380 2.49e-13 69.3
MsaG008385 MtrunA17_Chr8g0369161 40.506 79 46 1 181 258 296 374 5.22e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008385 AT4G36730.2 45.000 80 44 0 191 270 220 299 1.03e-16 79.0
MsaG008385 AT4G36730.1 45.000 80 44 0 191 270 222 301 1.09e-16 79.0
MsaG008385 AT2G35530.1 41.558 77 44 1 183 258 296 372 2.32e-13 70.1

Find 55 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGGGCTGGGTCAGAATT+TGG 0.147766 2:-33568324 MsaT008385.1:CDS
GCAAATAATTTGTTGAAATC+TGG 0.194573 2:+33569114 None:intergenic
CACAAGTTTCTCGAGCTTAA+TGG 0.203537 2:-33569669 MsaT008385.1:CDS
TCTGGGCTGGGTCAGAATTT+GGG 0.213477 2:-33568323 MsaT008385.1:CDS
CATTGAAGTAGTGCAATATT+TGG 0.230581 2:+33569484 None:intergenic
CAGAGATCTAACATGATTAT+TGG 0.278706 2:-33568462 MsaT008385.1:CDS
AAACGCTCGCTGAGATTTCT+AGG 0.285624 2:-33567588 MsaT008385.1:CDS
CTCTGCTACATGAGGATTAT+AGG 0.311040 2:+33569432 None:intergenic
TCTCAGCGAGCGTTTGTGTT+AGG 0.317910 2:+33567596 None:intergenic
AAATAAGAAAAGAACGAAAA+AGG 0.341051 2:-33567811 MsaT008385.1:CDS
TTCTTTGTAGAAAATCCATC+TGG 0.342852 2:-33568341 MsaT008385.1:intron
CTTCTGCATCAAAACATGAT+AGG 0.360568 2:-33569029 MsaT008385.1:CDS
CAAATAATTTGTTGAAATCT+GGG 0.364865 2:+33569115 None:intergenic
GAAGAGCTAGTTAAAGAGTA+TGG 0.380462 2:-33567256 MsaT008385.1:CDS
AATAGATCTGCAACCAATTC+TGG 0.383766 2:+33567232 None:intergenic
TCTTGATGCTCAACTAAAAC+TGG 0.396539 2:-33568267 MsaT008385.1:intron
GATCTCTGCTTCATACTTGA+AGG 0.401624 2:+33568477 None:intergenic
TAAAGAGTATGGACCAGAAT+TGG 0.406806 2:-33567245 MsaT008385.1:CDS
GAATTTGGGGACAGTTGTTA+AGG 0.407905 2:-33568309 MsaT008385.1:CDS
TGGCAGACCTTACAATTTCT+CGG 0.413188 2:-33569624 MsaT008385.1:CDS
AAGAAAGAATCCATTCCATT+TGG 0.414690 2:-33569644 MsaT008385.1:CDS
GTTTATCTGCGCAGGGCGGC+TGG 0.415010 2:-33568923 MsaT008385.1:intron
CCAGTAATTAAACTTAAAAC+AGG 0.417895 2:+33569078 None:intergenic
AAGATCAAAGATAAGGAAAC+AGG 0.427615 2:-33567765 MsaT008385.1:intron
CTTAAACATCCGACCTGAAA+AGG 0.445249 2:+33570122 None:intergenic
TCTGCGTACCATTTCTGAAA+AGG 0.446864 2:+33569143 None:intergenic
TGTAGAAAATCCATCTGGGC+TGG 0.452613 2:-33568336 MsaT008385.1:intron
CTGGGCTGGGTCAGAATTTG+GGG 0.465599 2:-33568322 MsaT008385.1:CDS
CAGCTAAAAGATCAAAGATA+AGG 0.486050 2:-33567772 MsaT008385.1:CDS
TGAGATTTCTAGGAAATGTC+TGG 0.510510 2:-33567578 MsaT008385.1:CDS
TTCTGCATCAAAACATGATA+GGG 0.510843 2:-33569028 MsaT008385.1:CDS
GAGATGAACACTGGATTGCC+AGG 0.513260 2:-33570146 MsaT008385.1:CDS
GTAGAAAATCCATCTGGGCT+GGG 0.520703 2:-33568335 MsaT008385.1:intron
AAATTGTAAGGTCTGCCAAA+TGG 0.525325 2:+33569629 None:intergenic
AAATTCTGACCCAGCCCAGA+TGG 0.526322 2:+33568326 None:intergenic
TATCTGCGCAGGGCGGCTGG+TGG 0.532947 2:-33568920 MsaT008385.1:intron
AGGATTATAGGAGTGCTCCA+TGG 0.533304 2:+33569444 None:intergenic
CTTTCATCAGAGATGAACAC+TGG 0.545174 2:-33570155 None:intergenic
AGCGGCGCCGAGAAATTGTA+AGG 0.545305 2:+33569617 None:intergenic
GTAAGGTCTGCCAAATGGAA+TGG 0.548289 2:+33569634 None:intergenic
AATATTTATTCAGGAAGCTA+CGG 0.551586 2:-33569696 MsaT008385.1:intron
CATCCGACCTGAAAAGGTCC+TGG 0.555102 2:+33570128 None:intergenic
GGATTATAGGAGTGCTCCAT+GGG 0.557237 2:+33569445 None:intergenic
TCTAACATGATTATTGGTGA+TGG 0.571130 2:-33568456 MsaT008385.1:intron
CGATGAAAACAATTCTATTG+TGG 0.572017 2:-33567548 MsaT008385.1:intron
TCTTTGTAGAAAATCCATCT+GGG 0.584157 2:-33568340 MsaT008385.1:intron
GCATCAAAACATGATAGGGA+TGG 0.584620 2:-33569024 MsaT008385.1:CDS
AGCGAGCGTTTGTGTTAGGA+CGG 0.595395 2:+33567600 None:intergenic
CTATAATCCTCATGTAGCAG+AGG 0.614984 2:-33569431 MsaT008385.1:intron
CGACCCACCTCTGCTACATG+AGG 0.618025 2:+33569424 None:intergenic
TGATGCTCTACATGATCCCA+TGG 0.619943 2:-33569461 MsaT008385.1:CDS
GAACAAACTGTACCTCATTG+AGG 0.623003 2:+33568999 None:intergenic
GGAAGTAAGAGATCAACAGA+TGG 0.635178 2:-33568899 MsaT008385.1:CDS
CATGATTTACTGACATCTAG+CGG 0.690857 2:+33569599 None:intergenic
ATAGGGATGGAACCTCAATG+AGG 0.730132 2:-33569011 MsaT008385.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 33567213 33570165 33567213 ID=MsaG008385
Chr2 mRNA 33567213 33570165 33567213 ID=MsaT008385.1;Parent=MsaG008385
Chr2 exon 33567213 33567278 33567213 ID=MsaT008385.1.exon10;Parent=MsaT008385.1
Chr2 CDS 33567213 33567278 33567213 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33567549 33567674 33567549 ID=MsaT008385.1.exon9;Parent=MsaT008385.1
Chr2 CDS 33567549 33567674 33567549 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33567766 33567843 33567766 ID=MsaT008385.1.exon8;Parent=MsaT008385.1
Chr2 CDS 33567766 33567843 33567766 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33568268 33568353 33568268 ID=MsaT008385.1.exon7;Parent=MsaT008385.1
Chr2 CDS 33568268 33568353 33568268 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33568457 33568505 33568457 ID=MsaT008385.1.exon6;Parent=MsaT008385.1
Chr2 CDS 33568457 33568505 33568457 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33568877 33568931 33568877 ID=MsaT008385.1.exon5;Parent=MsaT008385.1
Chr2 CDS 33568877 33568931 33568877 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33569012 33569178 33569012 ID=MsaT008385.1.exon4;Parent=MsaT008385.1
Chr2 CDS 33569012 33569178 33569012 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33569432 33569512 33569432 ID=MsaT008385.1.exon3;Parent=MsaT008385.1
Chr2 CDS 33569432 33569512 33569432 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33569613 33569705 33569613 ID=MsaT008385.1.exon2;Parent=MsaT008385.1
Chr2 CDS 33569613 33569705 33569613 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Chr2 exon 33570136 33570165 33570136 ID=MsaT008385.1.exon1;Parent=MsaT008385.1
Chr2 CDS 33570136 33570165 33570136 ID=cds.MsaT008385.1;Parent=MsaT008385.1
Gene Sequence

>MsaT008385.1

ATGAACACTGGATTGCCAGGACCTTTTCAGGAAGCTACGGTATTCACAAGTTTCTCGAGCTTAATGGAGAAGAAAGAATCCATTCCATTTGGCAGACCTTACAATTTCTCGGCGCCGCTAGATCAAACTCCAAATATTGCACTACTTCAATGTGATGCTCTACATGATCCCATGGAGCACTCCTATAATCCTCATGTAGCAGAGGATGCTGCACTGGCCTTTTCAGAAATGGTACGCAGAAGAAACCCAGATTTCAACAAATTATTTGCTAGAAACTCAGAGCCTGTTTTAAGTTTAATTACTGGTGAATCGAGAGAATATGCCAACCAGTCTTCTGCATCAAAACATGATAGGGATGGAACCTCAATGAGGGCGGCTGGTGGAAGTAAGAGATCAACAGATGGAGATCAAGATGATAGTAACAAAGACTATCCTTCAAGTATGAAGCAGAGATCTAACATGATTATTGGTGATGAAAATCCATCTGGGCTGGGTCAGAATTTGGGGACAGTTGTTAAGGAATCTGACACTGACATTGATCTTGATGCTCAACTAAAACTGGAAGCTGATGAAATAAGAAAAGAACGAAAAAGGCTGTCAAACAGGAAATCAGCTAAAAGATCAAAGATAAGGAAACAGAAAGAATGTGAAGAGCTATGTCAAAAGATAGACACTCTTAAAGATGAAAATGCCGTCCTAACACAAACGCTCGCTGAGATTTCTAGGAAATGTCTGGAGCTCACCGATGAAAACAATTCTATTGTGGAAGAGCTAGTTAAAGAGTATGGACCAGAATTGGTTGCAGATCTATTGCACATGAAGCCTACTTGA
Protein sequence

>MsaT008385.1

MNTGLPGPFQEATVFTSFSSLMEKKESIPFGRPYNFSAPLDQTPNIALLQCDALHDPMEHSYNPHVAEDAALAFSEMVRRRNPDFNKLFARNSEPVLSLITGESREYANQSSASKHDRDGTSMRAAGGSKRSTDGDQDDSNKDYPSSMKQRSNMIIGDENPSGLGQNLGTVVKESDTDIDLDAQLKLEADEIRKERKRLSNRKSAKRSKIRKQKECEELCQKIDTLKDENAVLTQTLAEISRKCLELTDENNSIVEELVKEYGPELVADLLHMKPT*