AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008424


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008424 MtrunA17_Chr7g0274141 51.493 134 45 3 43 176 162 275 1.19e-23 98.6
MsaG008424 MtrunA17_Chr5g0401301 89.130 46 5 0 219 264 333 378 1.58e-22 95.9
MsaG008424 MtrunA17_Chr1g0206881 97.500 40 1 0 171 210 191 230 7.02e-19 85.5
MsaG008424 MtrunA17_Chr4g0054511 97.500 40 1 0 171 210 191 230 7.18e-19 85.5
MsaG008424 MtrunA17_Chr3g0140821 97.500 40 1 0 171 210 191 230 7.39e-19 85.5
MsaG008424 MtrunA17_Chr8g0378821 97.500 40 1 0 171 210 191 230 8.15e-19 85.5
MsaG008424 MtrunA17_Chr7g0253411 92.500 40 3 0 171 210 191 230 8.19e-18 82.4
MsaG008424 MtrunA17_Chr1g0190761 48.031 127 43 4 48 170 177 284 1.61e-16 78.6
MsaG008424 MtrunA17_Chr6g0461281 82.051 39 7 0 172 210 193 231 7.35e-15 73.9
MsaG008424 MtrunA17_Chr2g0300671 78.947 38 8 0 171 208 186 223 1.14e-12 67.4
MsaG008424 MtrunA17_Chr2g0300491 78.947 38 8 0 171 208 186 223 1.18e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008424 AT5G19780.1 97.500 40 1 0 171 210 191 230 1.20e-18 85.5
MsaG008424 AT5G19770.1 97.500 40 1 0 171 210 191 230 1.20e-18 85.5
MsaG008424 AT1G64740.1 94.872 39 2 0 171 209 191 229 1.44e-17 82.4
MsaG008424 AT4G14960.1 90.000 40 4 0 171 210 191 230 1.68e-17 82.0
MsaG008424 AT4G14960.2 90.000 40 4 0 171 210 191 230 2.39e-17 81.6
MsaG008424 AT1G50010.1 90.000 40 4 0 171 210 191 230 3.81e-17 80.9
MsaG008424 AT1G04820.1 90.000 40 4 0 171 210 191 230 3.81e-17 80.9
MsaG008424 AT5G24800.1 35.821 134 60 3 48 175 88 201 3.26e-15 73.9

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAATCCTTTCTAATCTTT+TGG 0.173079 2:+34633472 MsaT008424.1:CDS
CTTTCAGGATGCTCCAAAAT+AGG 0.198060 2:+34633947 MsaT008424.1:intron
CAATTATCTTGAAGTGAAAA+TGG 0.219376 2:-34633535 None:intergenic
GTTTAGCTGATTCCCTATTT+TGG 0.286405 2:-34633960 None:intergenic
AAGGGAAGAGAAAGCTTCTC+AGG 0.359196 2:+34633756 MsaT008424.1:CDS
TGAAGGTGGTGCTGATTGTT+TGG 0.361551 2:+34634988 MsaT008424.1:CDS
TATTAGGTCTCTACATTAAG+AGG 0.369226 2:+34634137 MsaT008424.1:intron
CACTGATGTGGCCGTGCTCT+TGG 0.374923 2:+34634728 MsaT008424.1:CDS
GAGCTAGCAACCAATGTATC+AGG 0.384263 2:-34635043 None:intergenic
GCTTCTTGCAATGTTTCATC+AGG 0.394608 2:-34634947 None:intergenic
TCACTTCAAGATAATTGAAA+TGG 0.396522 2:+34633541 MsaT008424.1:CDS
TTTCAGGATGCTCCAAAATA+GGG 0.416451 2:+34633948 MsaT008424.1:intron
AGCCTGCTCTCCATCAATTC+CGG 0.422382 2:-34633506 None:intergenic
AGGCGATCTATGACATTTGT+AGG 0.426144 2:+34634757 MsaT008424.1:CDS
CAACTTATACCAACTTGAAC+AGG 0.431265 2:+34634802 MsaT008424.1:CDS
CAAGGTACCACCGGAATTGA+TGG 0.432913 2:+34633496 MsaT008424.1:CDS
CAGTACCTTACTCTTCTTGA+CGG 0.444119 2:-34633575 None:intergenic
CAGGGAGAGGTTAATATGAA+TGG 0.457103 2:+34633775 MsaT008424.1:CDS
TGAAACATTGCAAGAAGCAT+TGG 0.478730 2:+34634952 MsaT008424.1:CDS
TTGTTACAATCCTGATACAT+TGG 0.480675 2:+34635033 MsaT008424.1:CDS
TAGAACCGTCAAGAAGAGTA+AGG 0.491702 2:+34633570 MsaT008424.1:CDS
CTGTTCAAGTTGGTATAAGT+TGG 0.510056 2:-34634801 None:intergenic
CCTTGTCCAAAAGATTAGAA+AGG 0.511316 2:-34633478 None:intergenic
TCAATCATAGAGAAGCTTGT+CGG 0.517765 2:+34633450 MsaT008424.1:CDS
TTGGATTATGCTTGTGGTGA+AGG 0.518389 2:+34634971 MsaT008424.1:CDS
AGAAGCTTTCTCTTCCCTTG+AGG 0.522668 2:-34633752 None:intergenic
CACCGGAATTGATGGAGAGC+AGG 0.524298 2:+34633504 MsaT008424.1:CDS
AGAAGAGAAAGCATAAGACT+TGG 0.526009 2:-34634055 None:intergenic
AGAGAAAGCTTCTCAGGGAG+AGG 0.567096 2:+34633762 MsaT008424.1:CDS
AATGTTTCATCAGGAACACT+AGG 0.568085 2:-34634938 None:intergenic
TGATATCAACCTGTTCAAGT+TGG 0.577755 2:-34634811 None:intergenic
ACAATCAGGTCAACGACAAG+TGG 0.581189 2:+34633727 MsaT008424.1:intron
TCTTCTCGAGCACACTGATG+TGG 0.589746 2:+34634716 MsaT008424.1:CDS
GATTATGCTTGTGGTGAAGG+TGG 0.591952 2:+34634974 MsaT008424.1:CDS
CGCCTCATTATCCAAGAGCA+CGG 0.592409 2:-34634739 None:intergenic
GGCCGTGCTCTTGGATAATG+AGG 0.598449 2:+34634737 MsaT008424.1:CDS
AAATACTAAGCTTGAAACAC+AGG 0.600462 2:+34634010 MsaT008424.1:CDS
AGGGAAGAGAAAGCTTCTCA+GGG 0.602774 2:+34633757 MsaT008424.1:CDS
CAACGACAAGTGGATCCTCA+AGG 0.604416 2:+34633737 MsaT008424.1:CDS
GGAGATCTCTTGATATTGAG+AGG 0.607664 2:+34634778 MsaT008424.1:CDS
GGATTGTAACAATTCCCTTG+TGG 0.608596 2:-34635022 None:intergenic
TTGGAGATTACTACTCCACA+AGG 0.615549 2:+34635007 MsaT008424.1:CDS
AACGACAAGTGGATCCTCAA+GGG 0.631547 2:+34633738 MsaT008424.1:CDS
GAAGCATTGGATTATGCTTG+TGG 0.636390 2:+34634965 MsaT008424.1:CDS
CTGCTCTCCATCAATTCCGG+TGG 0.639314 2:-34633503 None:intergenic
TTTGATTCTTCAATGCACTG+AGG 0.654828 2:-34634913 None:intergenic
CTGATGCAAAACGAGTCAAG+AGG 0.683668 2:+34633809 MsaT008424.1:CDS
TGGAGATTACTACTCCACAA+GGG 0.734669 2:+34635008 MsaT008424.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 34632954 34635087 34632954 ID=MsaG008424
Chr2 mRNA 34632954 34635087 34632954 ID=MsaT008424.1;Parent=MsaG008424
Chr2 exon 34632954 34632956 34632954 ID=MsaT008424.1.exon1;Parent=MsaT008424.1
Chr2 CDS 34632954 34632956 34632954 ID=cds.MsaT008424.1;Parent=MsaT008424.1
Chr2 exon 34633449 34633591 34633449 ID=MsaT008424.1.exon2;Parent=MsaT008424.1
Chr2 CDS 34633449 34633591 34633449 ID=cds.MsaT008424.1;Parent=MsaT008424.1
Chr2 exon 34633735 34633830 34633735 ID=MsaT008424.1.exon3;Parent=MsaT008424.1
Chr2 CDS 34633735 34633830 34633735 ID=cds.MsaT008424.1;Parent=MsaT008424.1
Chr2 exon 34633954 34634086 34633954 ID=MsaT008424.1.exon4;Parent=MsaT008424.1
Chr2 CDS 34633954 34634086 34633954 ID=cds.MsaT008424.1;Parent=MsaT008424.1
Chr2 exon 34634143 34634268 34634143 ID=MsaT008424.1.exon5;Parent=MsaT008424.1
Chr2 CDS 34634143 34634268 34634143 ID=cds.MsaT008424.1;Parent=MsaT008424.1
Chr2 exon 34634699 34634823 34634699 ID=MsaT008424.1.exon6;Parent=MsaT008424.1
Chr2 CDS 34634699 34634823 34634699 ID=cds.MsaT008424.1;Parent=MsaT008424.1
Chr2 exon 34634907 34635087 34634907 ID=MsaT008424.1.exon7;Parent=MsaT008424.1
Chr2 CDS 34634907 34635087 34634907 ID=cds.MsaT008424.1;Parent=MsaT008424.1
Gene Sequence

>MsaT008424.1

ATGCTCAATCATAGAGAAGCTTGTCGGACAATCCTTTCTAATCTTTTGGACAAGGTACCACCGGAATTGATGGAGAGCAGGCTATATATCCATTTTCACTTCAAGATAATTGAAATGGTGATTTTAGAACCGTCAAGAAGAGTAAGGTCAACGACAAGTGGATCCTCAAGGGAAGAGAAAGCTTCTCAGGGAGAGGTTAATATGAATGGAGACAAGAACCCTGATGCAAAACGAGTCAAGAGGATGCTCCAAAATAGGGAATCAGCTAAACGCTCGAGAGATAGAAAGATAGCTGAAAAAATACTAAGCTTGAAACACAGGTCTGCATGCATGCTTTTTAAATCCAAGTCTTATGCTTTCTCTTCTCTGATCTCTGTCTCTACATTAAGAGGTGATAATTCTTCATTGTTGAAGCGTTTATCAGATGTGAGTCAAAAATATGACAATTCTGCTGTTGACAACAGAATATTAAACGCTGATTTTGAAACAATAAAAGCGAAGTGTTCTATGACACATTCTCTTCTCGAGCACACTGATGTGGCCGTGCTCTTGGATAATGAGGCGATCTATGACATTTGTAGGAGATCTCTTGATATTGAGAGGCCAACTTATACCAACTTGAACAGATTCAACCTCAGTGCATTGAAGAATCAAAAGCCTAGTGTTCCTGATGAAACATTGCAAGAAGCATTGGATTATGCTTGTGGTGAAGGTGGTGCTGATTGTTTGGAGATTACTACTCCACAAGGGAATTGTTACAATCCTGATACATTGGTTGCTAGCTCATGCTTCTTATGCATTCAATAG

Protein sequence

>MsaT008424.1

MLNHREACRTILSNLLDKVPPELMESRLYIHFHFKIIEMVILEPSRRVRSTTSGSSREEKASQGEVNMNGDKNPDAKRVKRMLQNRESAKRSRDRKIAEKILSLKHRSACMLFKSKSYAFSSLISVSTLRGDNSSLLKRLSDVSQKYDNSAVDNRILNADFETIKAKCSMTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRFNLSALKNQKPSVPDETLQEALDYACGEGGADCLEITTPQGNCYNPDTLVASSCFLCIQ*