AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008446

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008446 MtrunA17_Chr2g0303101 96.300 1000 37 0 1 1000 4 1003 0.0 1997
MsaG008446 MtrunA17_Chr8g0380981 59.697 1057 352 16 11 998 2 1053 0.0 1179
MsaG008446 MtrunA17_Chr1g0189211 58.176 581 229 6 24 595 125 700 0.0 687
MsaG008446 MtrunA17_Chr1g0189211 37.603 242 139 3 759 999 998 1228 4.68e-39 158
MsaG008446 MtrunA17_Chr7g0261401 45.391 575 290 8 31 587 99 667 4.38e-159 490
MsaG008446 MtrunA17_Chr5g0397501 40.749 427 205 8 151 565 288 678 2.76e-87 306
MsaG008446 MtrunA17_Chr5g0397501 55.405 74 29 2 44 117 23 92 3.92e-14 77.4
MsaG008446 MtrunA17_Chr5g0410721 34.975 406 179 10 46 437 86 420 7.96e-59 217
MsaG008446 MtrunA17_Chr3g0116441 33.827 405 195 10 49 442 18 360 2.44e-55 210
MsaG008446 MtrunA17_Chr1g0164731 29.187 603 322 17 45 628 41 557 5.51e-54 198
MsaG008446 MtrunA17_Chr1g0164761 31.450 407 186 11 45 442 4 326 4.99e-47 177
MsaG008446 MtrunA17_Chr1g0199511 39.574 235 138 3 261 492 295 528 8.60e-43 170
MsaG008446 MtrunA17_Chr7g0214951 40.870 115 43 4 291 397 104 201 7.47e-16 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008446 AT1G30810.3 63.350 603 204 6 1 594 6 600 0.0 746
MsaG008446 AT1G30810.3 53.631 179 73 4 763 939 648 818 1.36e-50 192
MsaG008446 AT1G30810.2 63.350 603 204 6 1 594 6 600 0.0 746
MsaG008446 AT1G30810.2 53.631 179 73 4 763 939 648 818 1.36e-50 192
MsaG008446 AT1G30810.1 63.350 603 204 6 1 594 6 600 0.0 746
MsaG008446 AT1G30810.1 53.631 179 73 4 763 939 648 818 1.36e-50 192
MsaG008446 AT4G20400.1 60.274 584 219 4 5 582 12 588 0.0 719
MsaG008446 AT4G20400.1 40.000 230 109 4 776 997 743 951 1.44e-43 172
MsaG008446 AT2G34880.1 57.653 588 222 9 4 582 14 583 0.0 695
MsaG008446 AT2G34880.1 47.150 193 94 3 750 940 620 806 1.82e-45 177
MsaG008446 AT4G20400.2 61.466 532 192 4 57 582 7 531 0.0 665
MsaG008446 AT4G20400.2 40.088 227 107 4 779 997 689 894 1.63e-43 171
MsaG008446 AT1G08620.2 56.379 580 244 3 30 602 127 704 0.0 651
MsaG008446 AT1G08620.2 38.991 218 131 2 759 976 974 1189 1.11e-39 160
MsaG008446 AT1G08620.4 56.379 580 244 3 30 602 127 704 0.0 651
MsaG008446 AT1G08620.4 38.991 218 131 2 759 976 974 1189 1.11e-39 160
MsaG008446 AT1G08620.1 56.379 580 244 3 30 602 127 704 0.0 651
MsaG008446 AT1G08620.1 38.991 218 131 2 759 976 974 1189 1.11e-39 160
MsaG008446 AT1G08620.3 56.379 580 244 3 30 602 141 718 0.0 650
MsaG008446 AT1G08620.3 38.991 218 131 2 759 976 988 1203 1.50e-39 160
MsaG008446 AT2G38950.1 39.520 625 324 11 19 625 76 664 2.33e-136 427
MsaG008446 AT1G63490.1 40.741 432 209 8 149 570 32 426 4.57e-91 316
MsaG008446 AT1G63490.4 32.955 704 364 21 149 829 285 903 3.96e-90 316
MsaG008446 AT1G63490.4 52.174 69 29 2 49 117 31 95 8.54e-12 70.1
MsaG008446 AT1G63490.2 40.741 432 209 8 149 570 285 679 5.86e-90 315
MsaG008446 AT1G63490.2 52.174 69 29 2 49 117 31 95 8.54e-12 70.1
MsaG008446 AT5G46910.2 33.137 510 241 17 46 539 100 525 9.79e-62 223
MsaG008446 AT5G46910.1 33.659 511 237 19 46 539 100 525 4.11e-61 224
MsaG008446 AT3G48430.1 31.648 455 263 11 49 492 20 437 4.63e-46 181
MsaG008446 AT5G04240.1 38.723 235 140 3 261 492 263 496 6.09e-41 164
MsaG008446 AT1G63490.3 36.555 238 128 4 338 570 1 220 1.84e-37 152

Find 202 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGATCAGTATGCACTTTC+TGG 0.098787 2:-35142998 MsaT008446.1:CDS
GCAATATGCTAGCCACTTTA+AGG 0.112299 2:-35143334 MsaT008446.1:CDS
AGTTCTTACTAAGGCATTTA+AGG 0.159985 2:-35141951 MsaT008446.1:intron
CCGAGGGCTCCCTTCGGTTT+TGG 0.197047 2:+35143253 None:intergenic
GACTTGGAAACTGGAGTATT+TGG 0.209261 2:-35143061 MsaT008446.1:CDS
TTCTGTCAAGCCTATAAGTT+TGG 0.258215 2:-35140946 MsaT008446.1:CDS
CGCAATGAGCTTAGCTCTTC+GGG 0.259835 2:+35139770 None:intergenic
TTCTTAAGGACAAAGATATC+TGG 0.272205 2:-35143581 MsaT008446.1:CDS
ACAACAGATCCCAAACTTAT+AGG 0.275338 2:+35140936 None:intergenic
CCCTCATCTTTAAGAATTGA+TGG 0.280365 2:+35142283 None:intergenic
GTAATTGATGCTGGACTTCT+AGG 0.284250 2:-35140705 MsaT008446.1:CDS
GTTTGAAAATCTAACCTCTT+TGG 0.284374 2:+35144719 None:intergenic
CCCTTTACCGTCCATTGTTC+AGG 0.287730 2:-35140267 MsaT008446.1:intron
TCTCCTACATGTTCCTGATT+TGG 0.290265 2:+35143452 None:intergenic
TTCACTTGCTCTTGAGTTAT+GGG 0.291488 2:-35141525 MsaT008446.1:CDS
ACCAGATCATTGAGTAGATT+TGG 0.300651 2:+35142445 None:intergenic
TGAAAGTCTTAGAAGTAAAA+AGG 0.310711 2:-35144121 MsaT008446.1:intron
TGTAAAGTTCAACGGCATTT+AGG 0.320348 2:+35142122 None:intergenic
TTGAATGAAACAAATGGAAT+TGG 0.322118 2:-35144745 None:intergenic
GGTTCCACCTTGCCTTCTTA+AGG 0.322457 2:-35143595 MsaT008446.1:CDS
CACTTTAAGGAATGCTACTT+TGG 0.328409 2:-35143321 MsaT008446.1:CDS
AAAGCAGAAGCATGGCTTCC+AGG 0.340144 2:+35142514 None:intergenic
ACACTTATCTGCTGAGGTTT+CGG 0.342775 2:+35140409 None:intergenic
GTGCTGAACAGTGCGATATA+CGG 0.343791 2:+35142255 None:intergenic
GATTGTCCCTCCAGCTTGTT+GGG 0.347186 2:-35143616 MsaT008446.1:CDS
CAGTTATCGATGACACTTCC+TGG 0.347721 2:+35139923 None:intergenic
TCTTCGGCACAAGCCTAAAA+AGG 0.349781 2:-35144163 MsaT008446.1:CDS
TGGTATTAATTTGACTGATC+AGG 0.356809 2:-35139861 MsaT008446.1:CDS
TTAAGACAAGAATATTTATC+AGG 0.357562 2:+35141650 None:intergenic
CTTGCAGAGCTAACTCTTCA+TGG 0.358880 2:-35142025 MsaT008446.1:CDS
GGATTGTCCCTCCAGCTTGT+TGG 0.359013 2:-35143617 MsaT008446.1:CDS
CACAATTCTTGTACATTTGA+TGG 0.361785 2:-35141107 MsaT008446.1:CDS
ATTCACTTGCTCTTGAGTTA+TGG 0.365297 2:-35141526 MsaT008446.1:CDS
GGTACTTCCTCGAACAAATC+AGG 0.367457 2:+35142466 None:intergenic
ACCACTCTGGGTTCAATTGT+GGG 0.368663 2:-35142198 MsaT008446.1:CDS
CTTATATTGTAGGTGTACTA+TGG 0.370021 2:-35143088 MsaT008446.1:intron
AGGAAATTGAAGGTGAATAT+TGG 0.376749 2:-35143227 MsaT008446.1:CDS
ACAGGTGCTTCATCAATTAT+AGG 0.379898 2:+35143829 None:intergenic
GCGCAATGAGCTTAGCTCTT+CGG 0.382376 2:+35139769 None:intergenic
TTTATTCATGCAAACCTCAT+TGG 0.385890 2:+35141469 None:intergenic
TATGGAGCGGACTTGGAAAC+TGG 0.386530 2:-35143070 MsaT008446.1:CDS
GAGCTTCTGAGAAGCATTAA+CGG 0.393541 2:-35140308 MsaT008446.1:CDS
TACCACTCTGGGTTCAATTG+TGG 0.397229 2:-35142199 MsaT008446.1:CDS
AATGCTTCTCAGAAGCTCTA+AGG 0.397411 2:+35140313 None:intergenic
CTACTGAATCACCCTTGTTA+TGG 0.412634 2:+35141249 None:intergenic
AGTTTGGGATCTGTTGTTCA+TGG 0.417652 2:-35140930 MsaT008446.1:CDS
AAACTGGTTGTGCGGGAACC+AGG 0.420135 2:-35141415 MsaT008446.1:CDS
TTTGTTCTGCTCATGCGAAA+TGG 0.421801 2:-35141618 MsaT008446.1:CDS
CCTGAACAATGGACGGTAAA+GGG 0.427142 2:+35140267 None:intergenic
TATCGCACTGTTCAGCACTC+TGG 0.427845 2:-35142250 MsaT008446.1:CDS
ACATAGCTACAGATTCTTGT+TGG 0.428494 2:+35140735 None:intergenic
GGTGTACTATGGAGCGGACT+TGG 0.428752 2:-35143077 MsaT008446.1:CDS
TGTTGCAGATATCAGCTAGA+TGG 0.432281 2:-35143873 MsaT008446.1:intron
TCACTAACTATACTTTCATT+CGG 0.438920 2:+35141323 None:intergenic
TCTGTCAAGCCTATAAGTTT+GGG 0.440166 2:-35140945 MsaT008446.1:CDS
ATCGCACTGTTCAGCACTCT+GGG 0.442116 2:-35142249 MsaT008446.1:CDS
ACTTTAAGGAATGCTACTTT+GGG 0.447518 2:-35143320 MsaT008446.1:CDS
CGTAGAAGGAACTCCAAATC+AGG 0.448607 2:-35143465 MsaT008446.1:CDS
ATCCATACCTAAGGGTTATC+CGG 0.453266 2:-35143017 MsaT008446.1:CDS
GATGAAATCAATACTACAAA+AGG 0.455907 2:-35139798 MsaT008446.1:CDS
ACCAAATCTACTCAATGATC+TGG 0.458628 2:-35142446 MsaT008446.1:intron
ACTTTGGTGACTCAAAGATA+TGG 0.460598 2:-35142546 MsaT008446.1:CDS
CCTCCTCCGAGGGCTCCCTT+CGG 0.462526 2:+35143247 None:intergenic
GAGAAATACGGATTTCAAGC+AGG 0.463346 2:-35143384 MsaT008446.1:CDS
AAATTGCTTGGAGCACTAGA+AGG 0.464388 2:-35141551 MsaT008446.1:CDS
TAAGGCATCATCCATACCTA+AGG 0.467936 2:-35143026 MsaT008446.1:CDS
AATGAGCTAAACAAATTGCT+TGG 0.468421 2:-35141563 MsaT008446.1:CDS
ATTAGCTTCAGAAGCTGACT+TGG 0.470647 2:+35143425 None:intergenic
GAAGGAAGCGAAAACGTAGA+AGG 0.471595 2:-35143479 MsaT008446.1:CDS
GTCATCAGTGTGCAGAGTAT+TGG 0.471615 2:-35139970 MsaT008446.1:CDS
ACTTGAGGTGCATCTGCAAA+TGG 0.473346 2:-35141078 MsaT008446.1:CDS
TCGCTTAATTATCTACACTT+TGG 0.473473 2:-35142562 MsaT008446.1:CDS
GTTTCTGAAGTAATTGATGC+TGG 0.475315 2:-35140714 MsaT008446.1:CDS
CTGACAGGCAAGGATAAAGA+TGG 0.478929 2:-35141810 MsaT008446.1:intron
GACTTATATCTATCTGCTGT+CGG 0.481917 2:-35141686 MsaT008446.1:CDS
CAAAGTAGCATTCCTTAAAG+TGG 0.482363 2:+35143322 None:intergenic
AGATATCTTTGTCCTTAAGA+AGG 0.486084 2:+35143583 None:intergenic
TTGAAAGATGCAAATGAAGA+TGG 0.489132 2:-35143297 MsaT008446.1:CDS
ATACTCTGCACACTGATGAC+TGG 0.492600 2:+35139973 None:intergenic
TTTGAAGGAAGTGACATCTC+AGG 0.493396 2:-35142929 MsaT008446.1:CDS
GACAAGGGTCATGAAGAAAC+TGG 0.493740 2:-35141431 MsaT008446.1:CDS
CAGGCGAAAGACAACATAGA+AGG 0.494236 2:-35139842 MsaT008446.1:CDS
CAGAAGTTTCATCATGCTCT+AGG 0.495798 2:-35141136 MsaT008446.1:CDS
CCAAAACCGAAGGGAGCCCT+CGG 0.495936 2:-35143253 MsaT008446.1:CDS
ATGACACTTCCTGGAGATGT+AGG 0.496695 2:+35139932 None:intergenic
TGCTGAACAGTGCGATATAC+GGG 0.497948 2:+35142256 None:intergenic
AGAAGCTACTGTGGGAAAGA+TGG 0.500316 2:-35141974 MsaT008446.1:CDS
GAAACTGGAGTATTTGGAAG+TGG 0.503820 2:-35143055 MsaT008446.1:CDS
ACCTGAACAATGGACGGTAA+AGG 0.506844 2:+35140266 None:intergenic
TCTTAAGGACAAAGATATCT+GGG 0.507226 2:-35143580 MsaT008446.1:CDS
CCTTCAATTTCCTCCTCCGA+GGG 0.507714 2:+35143237 None:intergenic
TCTGAAGCTGATGAGAAATA+CGG 0.508516 2:-35143396 MsaT008446.1:CDS
AAAGGTTTCCCTACATCTCC+AGG 0.510314 2:-35139941 MsaT008446.1:CDS
CCGAAGGGAGCCCTCGGAGG+AGG 0.511178 2:-35143247 MsaT008446.1:CDS
GCACTTGCCTGATTTGTTCG+AGG 0.511183 2:-35142473 MsaT008446.1:CDS
GTAGATAATTAAGCGAATAG+AGG 0.512964 2:+35142569 None:intergenic
TTCAGCGACTTAAAATGAGT+TGG 0.516196 2:+35141764 None:intergenic
CATGCAAACCTCATTGGCCT+CGG 0.519400 2:+35141475 None:intergenic
ATCAGTATGCACTTTCTGGT+TGG 0.522030 2:-35142994 MsaT008446.1:CDS
ACTGGGATCTTGAGCTTCGA+TGG 0.522302 2:+35139991 None:intergenic
CCAGAAAGTGCATACTGATC+CGG 0.524034 2:+35142998 None:intergenic
AAACCTCAGCAGATAAGTGT+TGG 0.527747 2:-35140406 MsaT008446.1:CDS
AACCTCAGCAGATAAGTGTT+GGG 0.527827 2:-35140405 MsaT008446.1:CDS
CAACAGAAGATGAAGGTGAT+AGG 0.529116 2:+35141165 None:intergenic
AAGCATGCAATATATCCAAA+AGG 0.529982 2:-35140891 MsaT008446.1:intron
AATAACTTCCCGCGACTACC+AGG 0.532194 2:-35142965 MsaT008446.1:CDS
AAGATATGGTATGGAGTACC+TGG 0.534279 2:-35142532 MsaT008446.1:CDS
GTTGCAGATATCAGCTAGAT+GGG 0.534301 2:-35143872 MsaT008446.1:intron
TACTCTGCACACTGATGACT+GGG 0.536048 2:+35139974 None:intergenic
CCCTCGGAGGAGGAAATTGA+AGG 0.537610 2:-35143237 MsaT008446.1:CDS
ATGACTATTTGACCTATCCC+TGG 0.541294 2:+35141397 None:intergenic
ATCCGGATAACCCTTAGGTA+TGG 0.541772 2:+35143015 None:intergenic
GGTGACTCAAAGATATGGTA+TGG 0.542015 2:-35142541 MsaT008446.1:CDS
ACCTTCAATTTCCTCCTCCG+AGG 0.543087 2:+35143236 None:intergenic
TACATTACCTGAACAATGGA+CGG 0.543477 2:+35140260 None:intergenic
CTGAACCTTATGGCATATGT+AGG 0.544086 2:-35143638 MsaT008446.1:CDS
CATGAAGAAACTGGTTGTGC+GGG 0.545026 2:-35141422 MsaT008446.1:CDS
AAGAATGATACCTTGAAAAG+AGG 0.546746 2:+35140681 None:intergenic
CAAGGTGGAACCCAACAAGC+TGG 0.548195 2:+35143606 None:intergenic
TACTGATCCGGATAACCCTT+AGG 0.548758 2:+35143010 None:intergenic
ATCAATTTGCTGAATACGTG+TGG 0.552299 2:+35143542 None:intergenic
TCCATCAATTCTTAAAGATG+AGG 0.553059 2:-35142284 MsaT008446.1:CDS
ATATGCCATAAGGTTCAGCT+AGG 0.555619 2:+35143643 None:intergenic
ACAATCCTACATATGCCATA+AGG 0.556142 2:+35143633 None:intergenic
CCATGCGTTAACCAATCGAC+AGG 0.557899 2:+35142145 None:intergenic
TAAAATGTTTCAGGTTACAA+TGG 0.561074 2:-35140456 MsaT008446.1:intron
TGACACTTCCTGGAGATGTA+GGG 0.563083 2:+35139933 None:intergenic
ATCACGGAGCAAAGGAGTCT+AGG 0.563455 2:-35140353 MsaT008446.1:CDS
TAAAGATATACTCGCGCTCG+TGG 0.563905 2:+35141004 None:intergenic
CTTCTTCTAACAGCATGTTG+AGG 0.565189 2:-35142599 MsaT008446.1:intron
CATGAAGAGTTAGCTCTGCA+AGG 0.567369 2:+35142026 None:intergenic
ATAGGCCGACATGCTTCAGA+TGG 0.567817 2:+35143847 None:intergenic
AATTCTTGTACATTTGATGG+TGG 0.570192 2:-35141104 MsaT008446.1:CDS
ACAAGAGGTGGATGGAGACA+AGG 0.570762 2:-35141447 MsaT008446.1:CDS
TGTTTACATTACCTGAACAA+TGG 0.571970 2:+35140256 None:intergenic
CTTCGGAATGGTTTCTGCCG+AGG 0.574133 2:-35141492 MsaT008446.1:CDS
AATGGAATTGGATGCCAAAG+AGG 0.575222 2:-35144733 MsaT008446.1:intron
GAAAGATGGAGTTCTTACTA+AGG 0.575446 2:-35141960 MsaT008446.1:CDS
TTCCCAACACTTATCTGCTG+AGG 0.577377 2:+35140403 None:intergenic
AACTGGTTGTGCGGGAACCA+GGG 0.577770 2:-35141414 MsaT008446.1:CDS
GTACAGAACCTGGTAGTCGC+GGG 0.577977 2:+35142957 None:intergenic
AAATTGAAGGTGAATATTGG+CGG 0.578021 2:-35143224 MsaT008446.1:CDS
AATACCTCAACTTCATCCGT+TGG 0.578922 2:+35143189 None:intergenic
CCATCAATTCTTAAAGATGA+GGG 0.579325 2:-35142283 MsaT008446.1:CDS
GTCTTCACAACAGAAGATGA+AGG 0.582987 2:+35141158 None:intergenic
CTAGTTCCATGGCTGTATGT+TGG 0.583650 2:-35142902 MsaT008446.1:CDS
TTGCATGAATAAACAAGAGG+TGG 0.585885 2:-35141459 MsaT008446.1:CDS
GTTGTGCGGGAACCAGGGAT+AGG 0.585912 2:-35141409 MsaT008446.1:CDS
AATGTTTCAGGTTACAATGG+AGG 0.588047 2:-35140453 MsaT008446.1:intron
TGAATGTAGCTCCTGTCGAT+TGG 0.588215 2:-35142156 MsaT008446.1:CDS
TCTTTGTCCTTAAGAAGGCA+AGG 0.591332 2:+35143588 None:intergenic
GATAGTCATAGACAAAGATA+AGG 0.592174 2:-35141288 MsaT008446.1:CDS
GGTTTCTGCCGAGGCCAATG+AGG 0.593742 2:-35141483 MsaT008446.1:CDS
TCTCAGGAGTACTAGTTCCA+TGG 0.597637 2:-35142913 MsaT008446.1:CDS
TTGTAAACTGTAAAGTTCAA+CGG 0.597924 2:+35142114 None:intergenic
AGAGCTAACTCTTCATGGAA+AGG 0.598601 2:-35142020 MsaT008446.1:CDS
GTTTCGGTGAAAGTGACACT+TGG 0.603807 2:+35140425 None:intergenic
TTGAAATGGAGAAGCTACTG+TGG 0.606319 2:-35141983 MsaT008446.1:CDS
ATGACAAAATCACGGAGCAA+AGG 0.607325 2:-35140361 MsaT008446.1:CDS
TCATGAAGAAACTGGTTGTG+CGG 0.608614 2:-35141423 MsaT008446.1:CDS
AGTCATAGACAAAGATAAGG+TGG 0.609140 2:-35141285 MsaT008446.1:CDS
CAAGAGGTGGATGGAGACAA+GGG 0.610819 2:-35141446 MsaT008446.1:CDS
ATGAAGAGTTAGCTCTGCAA+GGG 0.613991 2:+35142027 None:intergenic
ATGAATAAACAAGAGGTGGA+TGG 0.615332 2:-35141455 MsaT008446.1:CDS
CTTCTCAGAAGCTCTAAGGA+AGG 0.615628 2:+35140317 None:intergenic
TATTGCAGATAGCCATAACA+AGG 0.616747 2:-35141261 MsaT008446.1:CDS
AAACCGAAGGGAGCCCTCGG+AGG 0.616853 2:-35143250 MsaT008446.1:CDS
GCAGCCAACGGATGAAGTTG+AGG 0.619008 2:-35143193 MsaT008446.1:intron
AGAAATACGGATTTCAAGCA+GGG 0.620076 2:-35143383 MsaT008446.1:CDS
AGTACAGAACCTGGTAGTCG+CGG 0.621060 2:+35142956 None:intergenic
AAAGTCTTTAAACGTGAAGT+CGG 0.621637 2:+35143359 None:intergenic
TGAAATGGAGAAGCTACTGT+GGG 0.625781 2:-35141982 MsaT008446.1:CDS
CCTGTCGATTGGTTAACGCA+TGG 0.630097 2:-35142145 MsaT008446.1:CDS
ATAGTAACCACCAAAACCGA+AGG 0.630250 2:-35143263 MsaT008446.1:CDS
CACATGCCAACATACAGCCA+TGG 0.631048 2:+35142896 None:intergenic
AAGGCATCATCCATACCTAA+GGG 0.631276 2:-35143025 MsaT008446.1:CDS
GCGGATAGTTGAGCAGCCAA+CGG 0.631532 2:-35143205 MsaT008446.1:CDS
GGATAAAGATGGAAGAAGAG+AGG 0.633004 2:-35141799 MsaT008446.1:CDS
TAACAAGGGTGATTCAGTAG+AGG 0.633767 2:-35141246 MsaT008446.1:CDS
TCAAAACTTAGTACAGAACC+TGG 0.635235 2:+35142947 None:intergenic
GAGACTACATGACAAAATCA+CGG 0.643963 2:-35140369 MsaT008446.1:CDS
ACTCCAAATCAGGAACATGT+AGG 0.644682 2:-35143455 MsaT008446.1:CDS
ATTGTAGGTGTACTATGGAG+CGG 0.652824 2:-35143083 MsaT008446.1:intron
GTGGAACCCAACAAGCTGGA+GGG 0.653359 2:+35143610 None:intergenic
GGTGGAACCCAACAAGCTGG+AGG 0.654238 2:+35143609 None:intergenic
GGGATCCATCTGAAGCATGT+CGG 0.655114 2:-35143852 MsaT008446.1:CDS
TGCCATAAGGTTCAGCTAGG+GGG 0.655457 2:+35143646 None:intergenic
TAGGGCTACTTGAACAACAT+AGG 0.657747 2:+35139894 None:intergenic
CATGTGACAATGATGTCTTA+CGG 0.661845 2:+35142089 None:intergenic
CTGTTGTTCATGGAAAGCTA+TGG 0.661927 2:-35140920 MsaT008446.1:CDS
ATTTGATGGTGGAAAACTTG+AGG 0.662789 2:-35141093 MsaT008446.1:CDS
ATGCCATAAGGTTCAGCTAG+GGG 0.666477 2:+35143645 None:intergenic
CTGACGCACAATGTTCTGAG+TGG 0.675673 2:-35139733 MsaT008446.1:CDS
TATGCCATAAGGTTCAGCTA+GGG 0.679670 2:+35143644 None:intergenic
TAGTAACCACCAAAACCGAA+GGG 0.682346 2:-35143262 MsaT008446.1:CDS
GCAGAGTATTGGAATCACAA+AGG 0.682618 2:-35139959 MsaT008446.1:CDS
TGATGCTAAAGAAATTAACC+CGG 0.693704 2:+35140763 None:intergenic
GGTTTGCATGAATAAACAAG+AGG 0.696654 2:-35141462 MsaT008446.1:CDS
TTGTCCTTAAGAAGGCAAGG+TGG 0.697465 2:+35143591 None:intergenic
ACCCACAATTGAACCCAGAG+TGG 0.709442 2:+35142197 None:intergenic
TCGCACTGTTCAGCACTCTG+GGG 0.722408 2:-35142248 MsaT008446.1:CDS
ATTGCAGATAGCCATAACAA+GGG 0.737688 2:-35141260 MsaT008446.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 35139685 35144754 35139685 ID=MsaG008446
Chr2 mRNA 35139685 35144754 35139685 ID=MsaT008446.1;Parent=MsaG008446
Chr2 exon 35139685 35140014 35139685 ID=MsaT008446.1.exon11;Parent=MsaT008446.1
Chr2 CDS 35139685 35140014 35139685 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35140268 35140465 35140268 ID=MsaT008446.1.exon10;Parent=MsaT008446.1
Chr2 CDS 35140268 35140465 35140268 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35140692 35140795 35140692 ID=MsaT008446.1.exon9;Parent=MsaT008446.1
Chr2 CDS 35140692 35140795 35140692 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35140892 35141825 35140892 ID=MsaT008446.1.exon8;Parent=MsaT008446.1
Chr2 CDS 35140892 35141825 35140892 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35141952 35142320 35141952 ID=MsaT008446.1.exon7;Parent=MsaT008446.1
Chr2 CDS 35141952 35142320 35141952 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35142447 35142608 35142447 ID=MsaT008446.1.exon6;Parent=MsaT008446.1
Chr2 CDS 35142447 35142608 35142447 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35142877 35143098 35142877 ID=MsaT008446.1.exon5;Parent=MsaT008446.1
Chr2 CDS 35142877 35143098 35142877 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35143194 35143706 35143194 ID=MsaT008446.1.exon4;Parent=MsaT008446.1
Chr2 CDS 35143194 35143706 35143194 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35143810 35143887 35143810 ID=MsaT008446.1.exon3;Parent=MsaT008446.1
Chr2 CDS 35143810 35143887 35143810 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35144122 35144193 35144122 ID=MsaT008446.1.exon2;Parent=MsaT008446.1
Chr2 CDS 35144122 35144193 35144122 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Chr2 exon 35144734 35144754 35144734 ID=MsaT008446.1.exon1;Parent=MsaT008446.1
Chr2 CDS 35144734 35144754 35144734 ID=cds.MsaT008446.1;Parent=MsaT008446.1
Gene Sequence

>MsaT008446.1

ATGGAATTGGATGCCAAAGAGGATAGTCCTCTTCGGCACAAGCCTAAAAAGGACAATGCACTTGAGTCTCCTGAAAGTCTTAGAAGTAAAAAGATATCAGCTAGATGGGATCCATCTGAAGCATGTCGGCCTATAATTGATGAAGCACCTGTTTTTCATCCAACTATTGAAGAGTTTGAAGATACATTGAGTTACATAGCGAAGATACGCCCCCTAGCTGAACCTTATGGCATATGTAGGATTGTCCCTCCAGCTTGTTGGGTTCCACCTTGCCTTCTTAAGGACAAAGATATCTGGGAAAATGCTGAATTTTCCACACGTATTCAGCAAATTGATTTACTTCAAAATAGAGAACCTATGAAGAAAAAAAGTAGGGGAAGGAAGCGAAAACGTAGAAGGAACTCCAAATCAGGAACATGTAGGAGAAATTCCAAGTCAGCTTCTGAAGCTAATAACACTTCTGAAGCTGATGAGAAATACGGATTTCAAGCAGGGTCCGACTTCACGTTTAAAGACTTTCAGCAATATGCTAGCCACTTTAAGGAATGCTACTTTGGGTTGAAAGATGCAAATGAAGATGGAAAAGTTAACGATAGTAACCACCAAAACCGAAGGGAGCCCTCGGAGGAGGAAATTGAAGGTGAATATTGGCGGATAGTTGAGCAGCCAACGGATGAAGTTGAGGTGTACTATGGAGCGGACTTGGAAACTGGAGTATTTGGAAGTGGTTTTTCTAAGGCATCATCCATACCTAAGGGTTATCCGGATCAGTATGCACTTTCTGGTTGGAATCTAAATAACTTCCCGCGACTACCAGGTTCTGTACTAAGTTTTGAAGGAAGTGACATCTCAGGAGTACTAGTTCCATGGCTGTATGTTGGCATGTGCTTTTCATCGTTTTGTTGGCATGTTGAGGATCATCACCTCTATTCGCTTAATTATCTACACTTTGGTGACTCAAAGATATGGTATGGAGTACCTGGAAGCCATGCTTCTGCTTTAGAAAATGCAATGAAGAAGCACTTGCCTGATTTGTTCGAGGAAGTACCAAATCTACTCAATGATCTGGTTACTCAGTTATCTCCATCAATTCTTAAAGATGAGGGAGTGCCCGTATATCGCACTGTTCAGCACTCTGGGGAATTTGTCATTACTTTTCCGAGGGGGTACCACTCTGGGTTCAATTGTGGGTTCAACTGTGCAGAAGCAGTGAATGTAGCTCCTGTCGATTGGTTAACGCATGGCCTAAATGCCGTTGAACTTTACAGTTTACAACGCCGTAAGACATCATTGTCACATGACAAGCTTTTATTTGGATCATCGCTGGAAGCTATACGAGCCCTTGCAGAGCTAACTCTTCATGGAAAGGAAAGTTCAAAAAATTTGAAATGGAGAAGCTACTGTGGGAAAGATGGAGTTCTTACTAAGGCATTTAAGGCAAGGATAAAGATGGAAGAAGAGAGGCTAAACTGTCTTCCAACTCATTTTAAGTCGCTGAAGATGGGCAACGACTTTGACTTGCATACAGAACGAGAATGCTTTTCTTGCTTCTATGACTTATATCTATCTGCTGTCGGTTGCGAGTGCTCTCCTGATAAATATTCTTGTCTTAAGCATGCTAGTTTGTTCTGCTCATGCGAAATGGACAAAAGATTTGTTTTGCTTCGATATAATATGAATGAGCTAAACAAATTGCTTGGAGCACTAGAAGGAGATTCACTTGCTCTTGAGTTATGGGAAAGTAAAAACTTCGGAATGGTTTCTGCCGAGGCCAATGAGGTTTGCATGAATAAACAAGAGGTGGATGGAGACAAGGGTCATGAAGAAACTGGTTGTGCGGGAACCAGGGATAGGTCAAATAGTCATGCTACATCAGAGCTAATGCAGTGCGAATCTCAGCTTGTAACTCTTAGTGCGCCGAATGAAAGTATAGTTAGTGATAATGACAATATGATAGTCATAGACAAAGATAAGGTGGATATTGCAGATAGCCATAACAAGGGTGATTCAGTAGAGGAAAAAGCATGTTGTTCAGAGATAAAAAAGGAACAAGACAACATGGAACTTGTTGTTGACCTATCACCTTCATCTTCTGTTGTGAAGACAGAAGTTTCATCATGCTCTAGGAATGTTCACAATTCTTGTACATTTGATGGTGGAAAACTTGAGGTGCATCTGCAAATGGATTCAGATTCAAGAAAGAAACCCAAAATTGAAGTTGAAAAAGTCATTGACACCACGAGCGCGAGTATATCTTTAACACAAGAAAGCTTTCTAATGCAAATTTTGAGTACTTCTGTCAAGCCTATAAGTTTGGGATCTGTTGTTCATGGAAAGCTATGGTGCAATAAGCATGCAATATATCCAAAAGGATTCAAGAGCCGGGTTAATTTCTTTAGCATCATCGATCCAACAAGAATCTGTAGCTATGTTTCTGAAGTAATTGATGCTGGACTTCTAGGACCTCTTTTCAAGGTTACAATGGAGGAATGTCCAAGTGTCACTTTCACCGAAACCTCAGCAGATAAGTGTTGGGAATCAGTTCTTAAGAGACTACATGACAAAATCACGGAGCAAAGGAGTCTAGGTGAACTTGAACTTCCTTCCTTAGAGCTTCTGAGAAGCATTAACGGTTTTAGAATGTTCGGCTTCCCTTTACCGTCCATTGTTCAGGCCATCGAAGCTCAAGATCCCAGTCATCAGTGTGCAGAGTATTGGAATCACAAAGGTTTCCCTACATCTCCAGGAAGTGTCATCGATAACTGTAAAGTCCTATGTTGTTCAAGTAGCCCTACCAGAGTTTTTGGTATTAATTTGACTGATCAGGCGAAAGACAACATAGAAGGAAGTTATTATTCTCTTGAAGAGATGAAATCAATACTACAAAAGGCAAGTCCCGAAGAGCTAAGCTCATTGCGCAAGTTTCTCATCTCTGACGCACAATGTTCTGAGTGGAGAATGACATTAACATCATTGATCGATGAGATTCAGAAAGCTTTGTAG
Protein sequence

>MsaT008446.1

MELDAKEDSPLRHKPKKDNALESPESLRSKKISARWDPSEACRPIIDEAPVFHPTIEEFEDTLSYIAKIRPLAEPYGICRIVPPACWVPPCLLKDKDIWENAEFSTRIQQIDLLQNREPMKKKSRGRKRKRRRNSKSGTCRRNSKSASEANNTSEADEKYGFQAGSDFTFKDFQQYASHFKECYFGLKDANEDGKVNDSNHQNRREPSEEEIEGEYWRIVEQPTDEVEVYYGADLETGVFGSGFSKASSIPKGYPDQYALSGWNLNNFPRLPGSVLSFEGSDISGVLVPWLYVGMCFSSFCWHVEDHHLYSLNYLHFGDSKIWYGVPGSHASALENAMKKHLPDLFEEVPNLLNDLVTQLSPSILKDEGVPVYRTVQHSGEFVITFPRGYHSGFNCGFNCAEAVNVAPVDWLTHGLNAVELYSLQRRKTSLSHDKLLFGSSLEAIRALAELTLHGKESSKNLKWRSYCGKDGVLTKAFKARIKMEEERLNCLPTHFKSLKMGNDFDLHTERECFSCFYDLYLSAVGCECSPDKYSCLKHASLFCSCEMDKRFVLLRYNMNELNKLLGALEGDSLALELWESKNFGMVSAEANEVCMNKQEVDGDKGHEETGCAGTRDRSNSHATSELMQCESQLVTLSAPNESIVSDNDNMIVIDKDKVDIADSHNKGDSVEEKACCSEIKKEQDNMELVVDLSPSSSVVKTEVSSCSRNVHNSCTFDGGKLEVHLQMDSDSRKKPKIEVEKVIDTTSASISLTQESFLMQILSTSVKPISLGSVVHGKLWCNKHAIYPKGFKSRVNFFSIIDPTRICSYVSEVIDAGLLGPLFKVTMEECPSVTFTETSADKCWESVLKRLHDKITEQRSLGELELPSLELLRSINGFRMFGFPLPSIVQAIEAQDPSHQCAEYWNHKGFPTSPGSVIDNCKVLCCSSSPTRVFGINLTDQAKDNIEGSYYSLEEMKSILQKASPEELSSLRKFLISDAQCSEWRMTLTSLIDEIQKAL*