AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008457

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008457 MtrunA17_Chr2g0303181 83.815 173 25 1 1 170 1 173 1.03e-99 284
MsaG008457 MtrunA17_Chr8g0381011 71.023 176 45 1 1 170 1 176 1.67e-84 246
MsaG008457 MtrunA17_Chr4g0054701 57.273 110 47 0 2 111 31 140 3.53e-44 143
MsaG008457 MtrunA17_Chr4g0047341 45.333 75 41 0 41 115 40 114 1.31e-19 80.5
MsaG008457 MtrunA17_Chr4g0047341 36.250 80 50 1 4 83 42 120 8.51e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008457 AT4G20380.2 60.326 184 59 3 1 170 6 189 1.81e-60 186
MsaG008457 AT4G20380.7 60.326 184 59 3 1 170 6 189 1.81e-60 186
MsaG008457 AT4G20380.6 60.326 184 59 3 1 170 1 184 2.60e-60 185
MsaG008457 AT4G20380.3 60.326 184 59 3 1 170 1 184 2.60e-60 185
MsaG008457 AT4G20380.4 60.326 184 59 3 1 170 1 184 2.60e-60 185
MsaG008457 AT4G20380.1 60.326 184 59 3 1 170 1 184 2.60e-60 185
MsaG008457 AT4G20380.5 60.326 184 59 3 1 170 1 184 2.60e-60 185
MsaG008457 AT4G20380.8 52.857 210 59 3 1 170 1 210 4.36e-57 178
MsaG008457 AT1G32540.1 56.364 110 48 0 2 111 65 174 2.06e-43 142
MsaG008457 AT1G32540.3 56.364 110 48 0 2 111 32 141 7.59e-43 140
MsaG008457 AT1G32540.2 56.364 110 48 0 2 111 32 141 7.59e-43 140
MsaG008457 AT1G32540.5 56.481 108 47 0 2 109 32 139 3.26e-42 139
MsaG008457 AT4G20380.9 59.016 122 39 2 1 111 1 122 1.34e-41 136
MsaG008457 AT1G32540.7 62.857 70 26 0 2 71 65 134 9.43e-29 103
MsaG008457 AT1G32540.7 54.545 66 29 1 42 106 66 131 1.87e-14 67.0
MsaG008457 AT1G32540.4 62.857 70 26 0 2 71 32 101 1.85e-28 102
MsaG008457 AT1G32540.4 52.941 68 31 1 42 108 33 100 2.66e-14 65.9
MsaG008457 AT1G32540.6 62.857 70 26 0 2 71 32 101 1.85e-28 102
MsaG008457 AT1G32540.6 52.941 68 31 1 42 108 33 100 2.66e-14 65.9
MsaG008457 AT4G21610.1 40.625 96 57 0 40 135 57 152 3.31e-21 85.1
MsaG008457 AT4G21610.1 37.662 77 47 1 2 78 58 133 2.49e-11 59.3
MsaG008457 AT4G21610.2 47.222 72 38 0 40 111 57 128 5.78e-19 79.0

Find 41 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTGATTCAAGTGGGAAAT+TGG 0.251370 2:+35551127 MsaT008457.1:CDS
GACAGTTCCCACAATGTATT+TGG 0.260229 2:-35550642 None:intergenic
CACAATGTATTTGGTTCATT+TGG 0.272286 2:-35550633 None:intergenic
TCAGAGCTGGTTGTTACATT+AGG 0.293919 2:-35550922 None:intergenic
AACAGTTTGGTTCTCAAAGC+GGG 0.433810 2:-35551084 None:intergenic
CGGAACCACATTATGTGTAC+TGG 0.434315 2:+35549761 MsaT008457.1:CDS
TGTAACAACCAGCTCTGAAA+TGG 0.437855 2:+35550927 MsaT008457.1:CDS
CCACTTGAATCAACGGACAT+AGG 0.456065 2:-35551119 None:intergenic
AACATACCGGGAGGAAGAAC+AGG 0.457068 2:-35549854 None:intergenic
CAAACATTGCTTGCTCCTCT+AGG 0.458137 2:-35549806 None:intergenic
AGGATTTATAGTGTTACAAC+AGG 0.466530 2:-35550518 None:intergenic
CAGGTAAGCAATGTTGTTGT+GGG 0.474114 2:+35551603 MsaT008457.1:intron
AGAAGATACCATTTCAGAGC+TGG 0.501090 2:-35550935 None:intergenic
AAATGAACCAAATACATTGT+GGG 0.507138 2:+35550635 MsaT008457.1:CDS
CAATTTCCCACTTGAATCAA+CGG 0.526156 2:-35551126 None:intergenic
CCATATAGATGACTAATGTA+AGG 0.526995 2:+35550878 MsaT008457.1:intron
GAATTGTCACAGCTTTATTG+TGG 0.531778 2:+35550442 MsaT008457.1:CDS
GGAGCAAGCAATGTTTGTTG+TGG 0.536399 2:+35549812 MsaT008457.1:CDS
AAACATTGCTTGCTCCTCTA+GGG 0.538289 2:-35549805 None:intergenic
ACAACACTCATGTGTCCTCA+TGG 0.541151 2:+35550667 MsaT008457.1:CDS
ATATATATCATACCTGGTAC+AGG 0.541173 2:-35550538 None:intergenic
ATTACACCTGTTCTTCCTCC+CGG 0.553436 2:+35549848 MsaT008457.1:CDS
ACAGGTAAGCAATGTTGTTG+TGG 0.554197 2:+35551602 MsaT008457.1:intron
CAAATGAACCAAATACATTG+TGG 0.557893 2:+35550634 MsaT008457.1:CDS
AAATACATTGTGGGAACTGT+CGG 0.559035 2:+35550644 MsaT008457.1:CDS
TTGTCACAGCTTTATTGTGG+AGG 0.564317 2:+35550445 MsaT008457.1:CDS
GTTACATTAGGTCTCTGTGC+AGG 0.568554 2:-35550910 None:intergenic
ACATTATGTGTACTGGGTGT+AGG 0.584560 2:+35549768 MsaT008457.1:CDS
AGGTACACTTACACAAACAT+TGG 0.596374 2:-35550728 None:intergenic
AACATCTTGCTTTACCCTAG+AGG 0.603716 2:+35549791 MsaT008457.1:CDS
CAAATTGCACATTTGACTGA+GGG 0.609045 2:-35550694 None:intergenic
CCTATGTCCGTTGATTCAAG+TGG 0.615696 2:+35551119 MsaT008457.1:CDS
CAACAGTTTGGTTCTCAAAG+CGG 0.617036 2:-35551085 None:intergenic
AACACTATAAATCCTGTACC+AGG 0.634087 2:+35550526 MsaT008457.1:CDS
CTATGTCCGTTGATTCAAGT+GGG 0.636368 2:+35551120 MsaT008457.1:CDS
TACACCCAGTACACATAATG+TGG 0.638136 2:-35549766 None:intergenic
GGAACCACATTATGTGTACT+GGG 0.650157 2:+35549762 MsaT008457.1:CDS
ACAGTTTGGTTCTCAAAGCG+GGG 0.653740 2:-35551083 None:intergenic
ACAAATTGCACATTTGACTG+AGG 0.657368 2:-35550695 None:intergenic
TTGACTGAGGGAGCTCCATG+AGG 0.705071 2:-35550682 None:intergenic
ACTTTGCTAATGTACACACG+TGG 0.752607 2:+35550475 MsaT008457.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 35549758 35551647 35549758 ID=MsaG008457
Chr2 mRNA 35549758 35551647 35549758 ID=MsaT008457.1;Parent=MsaG008457
Chr2 exon 35549758 35549869 35549758 ID=MsaT008457.1.exon1;Parent=MsaT008457.1
Chr2 CDS 35549758 35549869 35549758 ID=cds.MsaT008457.1;Parent=MsaT008457.1
Chr2 exon 35550437 35550547 35550437 ID=MsaT008457.1.exon2;Parent=MsaT008457.1
Chr2 CDS 35550437 35550547 35550437 ID=cds.MsaT008457.1;Parent=MsaT008457.1
Chr2 exon 35550626 35550738 35550626 ID=MsaT008457.1.exon3;Parent=MsaT008457.1
Chr2 CDS 35550626 35550738 35550626 ID=cds.MsaT008457.1;Parent=MsaT008457.1
Chr2 exon 35550886 35550948 35550886 ID=MsaT008457.1.exon4;Parent=MsaT008457.1
Chr2 CDS 35550886 35550948 35550886 ID=cds.MsaT008457.1;Parent=MsaT008457.1
Chr2 exon 35551077 35551148 35551077 ID=MsaT008457.1.exon5;Parent=MsaT008457.1
Chr2 CDS 35551077 35551148 35551077 ID=cds.MsaT008457.1;Parent=MsaT008457.1
Chr2 exon 35551606 35551647 35551606 ID=MsaT008457.1.exon6;Parent=MsaT008457.1
Chr2 CDS 35551606 35551647 35551606 ID=cds.MsaT008457.1;Parent=MsaT008457.1
Gene Sequence

>MsaT008457.1

ATGCGGAACCACATTATGTGTACTGGGTGTAGGAACATCTTGCTTTACCCTAGAGGAGCAAGCAATGTTTGTTGTGGATTGTGCAACACAATTACACCTGTTCTTCCTCCCGGAATGGAATTGTCACAGCTTTATTGTGGAGGCTGTAGAACTTTGCTAATGTACACACGTGGAGCTACTAGCGTGAGATGTTCCTGTTGTAACACTATAAATCCTGTACCAGATTCTAACCAAATGAACCAAATACATTGTGGGAACTGTCGGACAACACTCATGTGTCCTCATGGAGCTCCCTCAGTCAAATGTGCAATTTGTCACTATATTACCAATGTTTGTATGACTAATGTAAGGTCATCAGTTCCTGCACAGAGACCTAATGTAACAACCAGCTCTGAAATGTCAAGACCCCGCTTTGAGAACCAAACTGTTGTAGTAGAAAATCCTATGTCCGTTGATTCAAGTGGGAAATTGGTAAGCAATGTTGTTGTGGGCGTTACAACACTGAAGAAATGA
Protein sequence

>MsaT008457.1

MRNHIMCTGCRNILLYPRGASNVCCGLCNTITPVLPPGMELSQLYCGGCRTLLMYTRGATSVRCSCCNTINPVPDSNQMNQIHCGNCRTTLMCPHGAPSVKCAICHYITNVCMTNVRSSVPAQRPNVTTSSEMSRPRFENQTVVVENPMSVDSSGKLVSNVVVGVTTLKK*