AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008525


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008525 MtrunA17_Chr8g0382121 94.077 287 16 1 1 286 27 313 0.0 570
MsaG008525 MtrunA17_Chr3g0123941 51.737 259 108 5 1 256 50 294 9.31e-83 252
MsaG008525 MtrunA17_Chr1g0200421 55.491 173 72 3 84 253 236 406 1.18e-64 210
MsaG008525 MtrunA17_Chr2g0320341 54.601 163 69 2 83 241 228 389 1.20e-62 202
MsaG008525 MtrunA17_Chr5g0422571 40.690 145 80 5 101 243 116 256 6.78e-24 98.2
MsaG008525 MtrunA17_Chr8g0373541 34.682 173 98 6 80 252 43 200 1.95e-21 94.0
MsaG008525 MtrunA17_Chr7g0263941 36.486 148 83 5 83 230 47 183 2.08e-21 93.2
MsaG008525 MtrunA17_Chr8g0342421 33.333 168 97 6 83 250 75 227 2.26e-21 93.6
MsaG008525 MtrunA17_Chr2g0326381 30.612 196 121 6 83 278 45 225 3.92e-21 92.4
MsaG008525 MtrunA17_Chr4g0029521 33.140 172 100 6 83 254 51 207 7.75e-21 92.0
MsaG008525 MtrunA17_Chr2g0321291 38.571 140 81 4 101 239 148 283 8.50e-21 90.1
MsaG008525 MtrunA17_Chr1g0198651 36.986 146 86 5 101 243 117 259 9.50e-21 89.7
MsaG008525 MtrunA17_Chr4g0053341 37.342 158 91 6 92 246 79 231 1.21e-20 88.2
MsaG008525 MtrunA17_Chr1g0191991 35.811 148 84 5 83 230 76 212 2.09e-20 90.9
MsaG008525 MtrunA17_Chr7g0268961 35.811 148 84 5 83 230 35 171 2.30e-20 90.5
MsaG008525 MtrunA17_Chr4g0026521 34.286 175 101 7 91 256 122 291 3.03e-20 88.2
MsaG008525 MtrunA17_Chr1g0181381 34.459 148 87 4 83 230 51 188 4.16e-20 89.7
MsaG008525 MtrunA17_Chr8g0352011 32.749 171 110 4 65 234 144 310 4.70e-20 88.6
MsaG008525 MtrunA17_Chr4g0040571 34.459 148 86 5 83 230 52 188 5.63e-20 89.4
MsaG008525 MtrunA17_Chr2g0330171 31.792 173 103 6 83 255 59 216 6.11e-20 89.4
MsaG008525 MtrunA17_Chr1g0185121 34.459 148 87 4 83 230 53 190 2.26e-19 87.4
MsaG008525 MtrunA17_Chr2g0328921 37.671 146 84 6 101 244 153 293 2.55e-19 86.3
MsaG008525 MtrunA17_Chr3g0141431 35.526 152 87 5 83 234 35 175 2.65e-19 87.0
MsaG008525 MtrunA17_Chr1g0210821 32.026 153 93 5 78 230 52 193 4.76e-19 87.0
MsaG008525 MtrunA17_Chr1g0151351 35.484 155 89 6 77 230 47 191 6.75e-19 86.3
MsaG008525 MtrunA17_Chr7g0258011 37.143 140 81 6 101 238 191 325 1.19e-18 84.7
MsaG008525 MtrunA17_Chr1g0199101 33.333 153 91 5 78 230 55 196 3.54e-18 84.3
MsaG008525 MtrunA17_Chr2g0324991 35.417 144 82 5 83 226 5 137 5.61e-18 79.0
MsaG008525 MtrunA17_Chr8g0375521 35.714 154 88 5 78 230 35 178 6.39e-18 83.2
MsaG008525 MtrunA17_Chr1g0162971 32.026 153 93 5 78 230 49 190 7.02e-18 83.2
MsaG008525 MtrunA17_Chr1g0173841 35.862 145 86 6 101 243 230 369 7.09e-18 82.8
MsaG008525 MtrunA17_Chr1g0163001 32.432 148 89 5 83 230 56 192 9.21e-18 83.2
MsaG008525 MtrunA17_Chr6g0479921 34.831 178 99 7 56 230 16 179 2.87e-17 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008525 AT1G34370.3 52.459 183 81 3 84 261 19 200 9.01e-65 205
MsaG008525 AT1G34370.2 52.459 183 81 3 84 261 229 410 1.58e-63 207
MsaG008525 AT1G34370.1 52.459 183 81 3 84 261 229 410 1.58e-63 207
MsaG008525 AT5G22890.1 48.768 203 99 2 52 250 170 371 1.80e-62 201
MsaG008525 AT1G08290.1 39.007 141 80 5 101 239 182 318 1.18e-21 93.2
MsaG008525 AT1G13290.1 37.415 147 87 4 101 245 103 246 2.04e-21 92.0
MsaG008525 AT5G60470.2 33.974 156 91 5 75 230 51 194 2.62e-21 93.6
MsaG008525 AT5G60470.3 33.974 156 91 5 75 230 47 190 2.88e-21 93.6
MsaG008525 AT5G66730.1 35.811 148 84 5 83 230 45 181 3.62e-21 93.2
MsaG008525 AT5G44160.1 34.459 148 86 5 83 230 50 186 4.05e-21 93.2
MsaG008525 AT5G44160.2 34.459 148 86 5 83 230 11 147 4.40e-21 92.8
MsaG008525 AT5G60470.1 33.557 149 89 4 82 230 47 185 4.60e-21 92.8
MsaG008525 AT1G51220.1 39.189 148 83 6 101 246 180 322 5.24e-21 91.7
MsaG008525 AT3G45260.2 31.395 172 104 5 83 254 52 209 5.36e-21 92.4
MsaG008525 AT3G45260.1 31.395 172 104 5 83 254 52 209 5.36e-21 92.4
MsaG008525 AT5G03150.1 35.616 146 84 4 85 230 68 203 7.20e-21 92.4
MsaG008525 AT1G03840.2 34.437 151 88 5 80 230 49 188 9.71e-21 92.0
MsaG008525 AT3G20880.1 37.107 159 93 6 101 257 258 411 2.21e-20 90.5
MsaG008525 AT1G03840.1 34.459 148 86 5 83 230 54 190 2.46e-20 90.9
MsaG008525 AT3G50700.1 35.503 169 92 7 62 230 32 183 3.61e-20 90.1
MsaG008525 AT1G55110.3 32.335 167 99 5 83 249 76 228 9.17e-20 89.0
MsaG008525 AT1G55110.1 32.335 167 99 5 83 249 76 228 9.17e-20 89.0
MsaG008525 AT1G55110.2 32.335 167 99 5 83 249 76 228 9.17e-20 89.0
MsaG008525 AT3G13810.4 34.459 148 87 4 83 230 83 220 2.88e-19 87.8
MsaG008525 AT3G13810.1 34.459 148 87 4 83 230 83 220 2.88e-19 87.8
MsaG008525 AT3G13810.5 34.459 148 87 4 83 230 78 215 3.44e-19 87.8
MsaG008525 AT3G13810.6 34.459 148 87 4 83 230 64 201 3.46e-19 87.4
MsaG008525 AT3G13810.2 34.459 148 87 4 83 230 84 221 4.21e-19 87.4
MsaG008525 AT3G13810.3 34.459 148 87 4 83 230 70 207 4.71e-19 87.0
MsaG008525 AT2G02070.2 32.026 153 93 5 78 230 60 201 6.98e-19 87.0
MsaG008525 AT2G02070.1 32.026 153 93 5 78 230 60 201 6.98e-19 87.0
MsaG008525 AT1G34790.1 35.329 167 97 8 92 252 137 298 1.02e-18 84.7
MsaG008525 AT2G02080.1 30.000 190 112 8 75 260 61 233 1.08e-18 86.3
MsaG008525 AT2G02080.4 30.000 190 112 8 75 260 61 233 1.08e-18 86.3
MsaG008525 AT2G02080.5 30.000 190 112 8 75 260 61 233 1.08e-18 86.3
MsaG008525 AT2G02080.3 30.000 190 112 8 75 260 61 233 1.08e-18 86.3
MsaG008525 AT4G02670.2 35.135 148 86 5 83 230 68 205 1.45e-18 85.5
MsaG008525 AT4G02670.1 35.135 148 86 5 83 230 66 203 1.47e-18 85.1
MsaG008525 AT1G14580.3 32.051 156 93 6 75 230 74 216 2.42e-18 85.1
MsaG008525 AT1G14580.1 32.051 156 93 6 75 230 60 202 2.46e-18 85.1
MsaG008525 AT1G14580.2 32.051 156 93 6 75 230 60 202 2.46e-18 85.1
MsaG008525 AT3G57670.1 35.862 145 86 6 101 243 219 358 8.79e-18 82.8
MsaG008525 AT2G01940.1 35.032 157 85 7 78 230 52 195 1.56e-16 79.7
MsaG008525 AT1G25250.3 34.899 149 87 5 83 230 46 185 1.79e-16 79.3
MsaG008525 AT2G02080.6 29.825 171 101 7 94 260 1 156 1.82e-16 79.3
MsaG008525 AT2G02080.2 29.825 171 101 7 94 260 1 156 1.82e-16 79.3
MsaG008525 AT1G25250.1 34.899 149 87 5 83 230 23 162 2.18e-16 78.6
MsaG008525 AT1G68130.1 34.899 149 86 5 83 230 54 192 4.38e-16 78.2
MsaG008525 AT2G01940.3 34.483 145 78 7 78 218 52 183 1.51e-13 70.9

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATGATTTGGATAAATTT+TGG 0.104736 2:-36705598 MsaT008525.1:CDS
GGAGGAATTGTTATTGTTTC+TGG 0.118268 2:+36706286 None:intergenic
GGAAGGTTTCTTTGATGATT+TGG 0.263746 2:-36705609 MsaT008525.1:CDS
CATGTCATCGTCAATGTAAT+TGG 0.286361 2:+36706231 None:intergenic
TCCACTTCTGCGAGATTTGC+GGG 0.292814 2:-36706138 MsaT008525.1:CDS
TTCCTCCAACCGAGTGTTAC+TGG 0.302145 2:-36706270 MsaT008525.1:CDS
TGCGTCGAGTTCCACAATTT+TGG 0.303992 2:+36706183 None:intergenic
ATGAAGGTTGCAAACGTAAC+AGG 0.321705 2:-36705982 MsaT008525.1:CDS
GACAATGACTTCTCTTGAAA+TGG 0.326614 2:+36705912 None:intergenic
AGTGAGTCTAAGTGTTGTTT+GGG 0.344560 2:+36706376 None:intergenic
TCTAGGAAGGAGAAATTGTT+TGG 0.374626 2:-36705767 MsaT008525.1:CDS
GACGAATTGTGGAGGAGATT+TGG 0.390719 2:+36706347 None:intergenic
GAGAAGTGTGTTTGTTTGAA+TGG 0.397224 2:+36706399 None:intergenic
GAGTGAGTCTAAGTGTTGTT+TGG 0.432423 2:+36706375 None:intergenic
GACGGCGGCGCCGGCAGAAG+AGG 0.442327 2:-36705684 MsaT008525.1:CDS
TTACGTTTGCAACCTTCATA+AGG 0.442930 2:+36705986 None:intergenic
TGTGTTTGTTTGAATGGATT+GGG 0.447482 2:+36706405 None:intergenic
GGACACGTGGAGCGGTTTGA+AGG 0.462859 2:-36705746 MsaT008525.1:CDS
TTCTGCGAGATTTGCGGGAA+AGG 0.464202 2:-36706133 MsaT008525.1:CDS
TTCCACTTCTGCGAGATTTG+CGG 0.470603 2:-36706139 MsaT008525.1:CDS
GTGTGTTTGTTTGAATGGAT+TGG 0.474691 2:+36706404 None:intergenic
AATTGTGGAGGAGATTTGGT+TGG 0.477694 2:+36706351 None:intergenic
TGATTGCATAGATACGTTAC+CGG 0.493467 2:-36705630 MsaT008525.1:CDS
GGAAAAGCAAGCGCGTTTAA+CGG 0.496000 2:-36706026 MsaT008525.1:CDS
AAAATCAATGACGGCGGCGC+CGG 0.497008 2:-36705693 MsaT008525.1:CDS
TTGGAATGGTCGTTATGAGT+GGG 0.500470 2:+36706202 None:intergenic
CCCACACCTTCCTCTTCTGC+CGG 0.501400 2:+36705674 None:intergenic
GAAGTTCAATCCGTTGAAAA+CGG 0.526797 2:-36705951 MsaT008525.1:CDS
AAATCATCAAAGAAACCTTC+CGG 0.527715 2:+36705611 None:intergenic
CCGGCGGTTGTTGAAGAGAA+AGG 0.528093 2:-36705716 MsaT008525.1:CDS
TCCCGCAAATCTCGCAGAAG+TGG 0.537900 2:+36706137 None:intergenic
GTCCAGTAACACTCGGTTGG+AGG 0.539855 2:+36706268 None:intergenic
GAGTCACGTGAAACAGTGTA+GGG 0.546406 2:-36705828 MsaT008525.1:CDS
GATGATGACTTGACGAATTG+TGG 0.554147 2:+36706336 None:intergenic
TGTTATTGTTTCTGGACAAG+TGG 0.555781 2:+36706294 None:intergenic
TAACGACCATTCCAAAATTG+TGG 0.557903 2:-36706194 MsaT008525.1:CDS
CGTATGCACATGCGCGCGCA+CGG 0.560123 2:-36706088 MsaT008525.1:CDS
GGAGAAATTGTTTGGACACG+TGG 0.562238 2:-36705759 MsaT008525.1:CDS
CCGGCAGAAGAGGAAGGTGT+GGG 0.568129 2:-36705674 MsaT008525.1:CDS
CAATTACATTGACGATGACA+TGG 0.570490 2:-36706230 MsaT008525.1:CDS
CGAAAACTCACACGTGCGAT+CGG 0.572334 2:-36705889 MsaT008525.1:CDS
CTCACACGTGCGATCGGTGT+CGG 0.577837 2:-36705883 MsaT008525.1:CDS
TGCATAGATACGTTACCGGA+AGG 0.579492 2:-36705626 MsaT008525.1:CDS
CCAACCGAGTGTTACTGGAC+AGG 0.586148 2:-36706265 MsaT008525.1:CDS
GCCGGCAGAAGAGGAAGGTG+TGG 0.587117 2:-36705675 MsaT008525.1:CDS
CCTTTCTCTTCAACAACCGC+CGG 0.591632 2:+36705716 None:intergenic
TTTGGAATGGTCGTTATGAG+TGG 0.596386 2:+36706201 None:intergenic
AGAGTCACGTGAAACAGTGT+AGG 0.598901 2:-36705829 MsaT008525.1:CDS
CCTGTCCAGTAACACTCGGT+TGG 0.599661 2:+36706265 None:intergenic
GCGGCGCCGGCAGAAGAGGA+AGG 0.609998 2:-36705680 MsaT008525.1:CDS
GCGGTTTGAAGGACACTCGC+CGG 0.611639 2:-36705735 MsaT008525.1:CDS
TCCACGTGGAAGTGTTCGTG+TGG 0.623338 2:-36705800 MsaT008525.1:CDS
GCAGAAGAGGAAGGTGTGGG+TGG 0.627397 2:-36705671 MsaT008525.1:CDS
ACCACCTGTCCAGTAACACT+CGG 0.627426 2:+36706261 None:intergenic
ATGAAGCGTTACGTAGCGTG+AGG 0.636493 2:-36706048 MsaT008525.1:CDS
AATTGTGGAACTCGACGCAG+TGG 0.638379 2:-36706179 MsaT008525.1:CDS
TTTCTCCTTCCTAGAAAACG+TGG 0.641293 2:+36705775 None:intergenic
AATTGTTTGGACACGTGGAG+CGG 0.644804 2:-36705754 MsaT008525.1:CDS
GTTTGAAGGACACTCGCCGG+CGG 0.649562 2:-36705732 MsaT008525.1:CDS
ACCGAGTGTTACTGGACAGG+TGG 0.653482 2:-36706262 MsaT008525.1:CDS
ACACGTGCGATCGGTGTCGG+AGG 0.660805 2:-36705880 MsaT008525.1:CDS
AGAGAAAGGAAAATCAATGA+CGG 0.662591 2:-36705702 MsaT008525.1:CDS
TGAAGCGTTACGTAGCGTGA+GGG 0.667496 2:-36706047 MsaT008525.1:CDS
GAAAGGAAAATCAATGACGG+CGG 0.669060 2:-36705699 MsaT008525.1:CDS
GCGCGCGCATGTGCATACGT+AGG 0.669149 2:+36706092 None:intergenic
GTGAGTCTAAGTGTTGTTTG+GGG 0.679107 2:+36706377 None:intergenic
AAGCGCGTTTAACGGCGACG+CGG 0.680564 2:-36706018 MsaT008525.1:CDS
AGTGTAGGGGAGAGTCCACG+TGG 0.705085 2:-36705814 MsaT008525.1:CDS
GATGACTTGACGAATTGTGG+AGG 0.731309 2:+36706339 None:intergenic
AGTCACGTGAAACAGTGTAG+GGG 0.737402 2:-36705827 MsaT008525.1:CDS
TCCACACGAACACTTCCACG+TGG 0.747159 2:+36705799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 36705592 36706452 36705592 ID=MsaG008525
Chr2 mRNA 36705592 36706452 36705592 ID=MsaT008525.1;Parent=MsaG008525
Chr2 exon 36705592 36706452 36705592 ID=MsaT008525.1.exon1;Parent=MsaT008525.1
Chr2 CDS 36705592 36706452 36705592 ID=cds.MsaT008525.1;Parent=MsaT008525.1
Gene Sequence

>MsaT008525.1

ATGGATTCTATTCAAAGCTTCATATCCCAATCCATTCAAACAAACACACTTCTCCCCAAACAACACTTAGACTCACTCTCCAACCAAATCTCCTCCACAATTCGTCAAGTCATCATCAACGCAACTGCTCTTCTCGCCACTTGTCCAGAAACAATAACAATTCCTCCAACCGAGTGTTACTGGACAGGTGGTGAGAAAACCAATTACATTGACGATGACATGGCAAATCCCACTCATAACGACCATTCCAAAATTGTGGAACTCGACGCAGTGGAACTGCTTGCGAAGCACTTCCACTTCTGCGAGATTTGCGGGAAAGGTTTCACGCGCGATGCAAACCTACGTATGCACATGCGCGCGCACGGAGAACAGTTCAAGACACATGAAGCGTTACGTAGCGTGAGGGAAAAGCAAGCGCGTTTAACGGCGACGCGGTTTTCTTGTCCTTATGAAGGTTGCAAACGTAACAGGTTACACAAGAAGTTCAATCCGTTGAAAACGGTGTTCTGTTTGAGAAACCATTTCAAGAGAAGTCATTGTCCGAAAACTCACACGTGCGATCGGTGTCGGAGGAAGAGTTTTGCGGTGGTGTCGGATTTGAAGAGTCACGTGAAACAGTGTAGGGGAGAGTCCACGTGGAAGTGTTCGTGTGGAACCACGTTTTCTAGGAAGGAGAAATTGTTTGGACACGTGGAGCGGTTTGAAGGACACTCGCCGGCGGTTGTTGAAGAGAAAGGAAAATCAATGACGGCGGCGCCGGCAGAAGAGGAAGGTGTGGGTGGATTTTTTACGAAGGAAAATGATTGCATAGATACGTTACCGGAAGGTTTCTTTGATGATTTGGATAAATTTTGGGTTTGA

Protein sequence

>MsaT008525.1

MDSIQSFISQSIQTNTLLPKQHLDSLSNQISSTIRQVIINATALLATCPETITIPPTECYWTGGEKTNYIDDDMANPTHNDHSKIVELDAVELLAKHFHFCEICGKGFTRDANLRMHMRAHGEQFKTHEALRSVREKQARLTATRFSCPYEGCKRNRLHKKFNPLKTVFCLRNHFKRSHCPKTHTCDRCRRKSFAVVSDLKSHVKQCRGESTWKCSCGTTFSRKEKLFGHVERFEGHSPAVVEEKGKSMTAAPAEEEGVGGFFTKENDCIDTLPEGFFDDLDKFWV*