AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008544


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008544 MtrunA17_Chr2g0303981 69.540 174 51 1 1 172 1 174 1.47e-83 248
MsaG008544 MtrunA17_Chr4g0059631 46.154 221 104 5 1 214 1 213 5.43e-60 190
MsaG008544 MtrunA17_Chr4g0022641 44.255 235 102 6 4 222 2 223 5.86e-56 184
MsaG008544 MtrunA17_Chr1g0212471 48.756 201 78 5 4 198 2 183 8.47e-56 177
MsaG008544 MtrunA17_Chr6g0457851 38.710 155 88 2 4 152 12 165 1.51e-31 119
MsaG008544 MtrunA17_Chr1g0212151 39.744 156 87 2 4 153 2 156 2.71e-31 118
MsaG008544 MtrunA17_Chr6g0449851 33.663 202 120 6 4 192 2 202 3.75e-30 114
MsaG008544 MtrunA17_Chr6g0449861 38.065 155 89 3 4 152 10 163 2.62e-26 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAATCCATCTATTGTTTC+TGG 0.138351 2:-37006252 None:intergenic
ACTTGGTAATGTTGCATATT+TGG 0.170747 2:+37006167 MsaT008544.1:CDS
GGAATGGGAAACTCAATTTA+TGG 0.183833 2:+37006215 MsaT008544.1:CDS
TCTTTCACATCAAAGATCTT+TGG 0.287719 2:-37005733 None:intergenic
ATGCAACAAAGTACTGAAAA+TGG 0.300561 2:+37005946 MsaT008544.1:CDS
CACAATCTCTTTGTGCACTT+TGG 0.338528 2:-37005797 None:intergenic
ATGATTACAAGCTTGAATCT+TGG 0.383659 2:-37005902 None:intergenic
TCCTGACCAGGTTTAGCAAC+TGG 0.388453 2:-37006195 None:intergenic
TCATGGATATGTTGCATTTG+AGG 0.410645 2:-37006060 None:intergenic
GCTTTGCCAGAAACAATAGA+TGG 0.415718 2:+37006246 MsaT008544.1:CDS
ACTTGGTGAAGTTGAAGAAT+TGG 0.421405 2:+37006116 MsaT008544.1:CDS
CTATTAAACACATTCAAGAT+TGG 0.421571 2:+37005512 MsaT008544.1:CDS
TGCTAAACCTGGTCAGGAAT+GGG 0.447809 2:+37006200 MsaT008544.1:CDS
TGTGAGGAGACAACATTATT+CGG 0.449930 2:-37006006 None:intergenic
ATGTTGTCTCCTCACATTCA+AGG 0.456551 2:+37006013 MsaT008544.1:CDS
TCCAGTTGCTAAACCTGGTC+AGG 0.458685 2:+37006194 MsaT008544.1:CDS
TGAGTTTCCCATTCCTGACC+AGG 0.483105 2:-37006207 None:intergenic
GAATCTTGTGGCATAGTCAT+TGG 0.501448 2:-37005652 None:intergenic
ATGAAGCATGGATGCCAAGT+TGG 0.507763 2:-37005832 None:intergenic
CAATCAAAGAAGCAATGGAT+TGG 0.512118 2:+37005555 MsaT008544.1:CDS
TTGCTAAACCTGGTCAGGAA+TGG 0.512431 2:+37006199 MsaT008544.1:CDS
CCTTCCATGTTCATGAAGCA+TGG 0.519687 2:-37005844 None:intergenic
GCATCCATGCTTCATGAACA+TGG 0.521603 2:+37005840 MsaT008544.1:CDS
ATACAAGCAACAAAAGATTG+AGG 0.523829 2:+37005702 MsaT008544.1:CDS
GTGGCTTCAAATGATCATCA+TGG 0.534306 2:-37006077 None:intergenic
GCTTGCTTAATTGTGTATGA+TGG 0.534651 2:+37005619 MsaT008544.1:CDS
GTTTAATAGTTAATTTCCCA+CGG 0.538383 2:-37005498 None:intergenic
TACAAGCAACAAAAGATTGA+GGG 0.545039 2:+37005703 MsaT008544.1:CDS
ACATTCAAGATTGGAGAGCT+CGG 0.553672 2:+37005521 MsaT008544.1:CDS
CCATGCTTCATGAACATGGA+AGG 0.554983 2:+37005844 MsaT008544.1:CDS
ACTTTCAATCAAAGAAGCAA+TGG 0.559273 2:+37005550 MsaT008544.1:CDS
GATTGGGCTAGTCAAGTACT+TGG 0.562422 2:+37006150 MsaT008544.1:CDS
GTAGAAATGGGTCGTGCCCG+TGG 0.563669 2:+37005481 None:intergenic
CATGCTTCATGAACATGGAA+GGG 0.576308 2:+37005845 MsaT008544.1:CDS
CGCATAATTGTCGAATCTTG+TGG 0.578423 2:-37005664 None:intergenic
ATGCTTCATGAACATGGAAG+GGG 0.583471 2:+37005846 MsaT008544.1:CDS
TTTAATAGTTAATTTCCCAC+GGG 0.586000 2:-37005497 None:intergenic
GATGATCCAGTTGCTAAACC+TGG 0.589750 2:+37006189 MsaT008544.1:CDS
ATCAAGCTTCCTTGAATGTG+AGG 0.590592 2:-37006022 None:intergenic
CTTAGTGATACTAATCGACT+TGG 0.592888 2:+37006099 MsaT008544.1:CDS
TGATGAAAATGTATCCAACT+TGG 0.601327 2:+37005818 MsaT008544.1:CDS
TTAATTGTGTATGATGGTGA+TGG 0.608708 2:+37005625 MsaT008544.1:CDS
TAGAAATGGGTCGTGCCCGT+GGG 0.613629 2:+37005482 None:intergenic
CACTACTCAGACGCAAAATG+TGG 0.617631 2:+37005981 MsaT008544.1:CDS
AGTCGATTAGTATCACTAAG+TGG 0.621773 2:-37006096 None:intergenic
CACAAGATTCGACAATTATG+CGG 0.681582 2:+37005665 MsaT008544.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 37005487 37006275 37005487 ID=MsaG008544
Chr2 mRNA 37005487 37006275 37005487 ID=MsaT008544.1;Parent=MsaG008544
Chr2 exon 37005487 37006275 37005487 ID=MsaT008544.1.exon1;Parent=MsaT008544.1
Chr2 CDS 37005487 37006275 37005487 ID=cds.MsaT008544.1;Parent=MsaT008544.1
Gene Sequence

>MsaT008544.1

ATGGGTCGTGCCCGTGGGAAATTAACTATTAAACACATTCAAGATTGGAGAGCTCGGAAATCAACTTTCAATCAAAGAAGCAATGGATTGGCAAAAAAAGTTTCTGAATTTTCTAGCAAGTTTGGAGTTGAAGCTTGCTTAATTGTGTATGATGGTGATGGTAGACCAATGACTATGCCACAAGATTCGACAATTATGCGGTCCATGCTTGAAAAATACAAGCAACAAAAGATTGAGGGTACAACTCCAAAGATCTTTGATGTGAAAGACTATTTTGCAAATAAGAAGAATAAGATTGAAGCTGATATTTCCAAAGTGCACAAAGAGATTGTGATGAAAATGTATCCAACTTGGCATCCATGCTTCATGAACATGGAAGGGGAACAGCTCAAAACTTTCATTGCTATTGTAGATGCCAAGATTCAAGCTTGTAATCATAAAATTAACATGCTAAAGAAAATGCAACAAAGTACTGAAAATGGTTTCATGCTAAACACTACTCAGACGCAAAATGTGGCTCCGAATAATGTTGTCTCCTCACATTCAAGGAAGCTTGATGTCACTGATAGATTTCCTCAAATGCAACATATCCATGATGATCATTTGAAGCCACTTAGTGATACTAATCGACTTGGTGAAGTTGAAGAATTGGATGATTTTATGGATTGGGCTAGTCAAGTACTTGGTAATGTTGCATATTTGGATGATCCAGTTGCTAAACCTGGTCAGGAATGGGAAACTCAATTTATGGATTTTGATGCTTTGCCAGAAACAATAGATGGATTTTAA

Protein sequence

>MsaT008544.1

MGRARGKLTIKHIQDWRARKSTFNQRSNGLAKKVSEFSSKFGVEACLIVYDGDGRPMTMPQDSTIMRSMLEKYKQQKIEGTTPKIFDVKDYFANKKNKIEADISKVHKEIVMKMYPTWHPCFMNMEGEQLKTFIAIVDAKIQACNHKINMLKKMQQSTENGFMLNTTQTQNVAPNNVVSSHSRKLDVTDRFPQMQHIHDDHLKPLSDTNRLGEVEELDDFMDWASQVLGNVAYLDDPVAKPGQEWETQFMDFDALPETIDGF*