AlfalfaGEDB | MsaG008547

M. sativa ssp. caerulea / MsaG008547

KEGG: -
GO: -
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008547	ABD33219.2	70.026	387	61	8	1	349	1	370	1.77e-168	484

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008547	Q2HRQ4	70.026	387	61	8	1	349	1	370	8.46e-169	484

TFs/TRs:

Gene ID	Type	Classification
MsaG008547	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001273	MsaG008547	0.802659	8.487265e-49	1.218927e-46
MsaG002885	MsaG008547	0.801836	1.251614e-48	1.764024e-46
MsaG003778	MsaG008547	0.803985	4.524184e-49	6.699370e-47
MsaG004987	MsaG008547	0.817032	7.075294e-52	1.440448e-49
MsaG005843	MsaG008547	0.822822	3.401209e-53	8.064362e-51
MsaG007312	MsaG008547	0.800840	1.997525e-48	2.752440e-46
MsaG008547	MsaG010978	0.815687	1.409615e-51	2.772778e-49
MsaG008547	MsaG012609	0.800375	2.482336e-48	3.384787e-46
MsaG008547	MsaG016974	0.803629	5.358550e-49	7.869665e-47
MsaG008547	MsaG018722	0.805559	2.130329e-49	3.271821e-47
MsaG008547	MsaG019395	0.825213	9.403780e-54	2.379384e-51
MsaG008547	MsaG020497	0.805763	1.931417e-49	2.980574e-47
MsaG008547	MsaG021301	0.806452	1.385187e-49	2.172854e-47
MsaG008547	MsaG024225	0.800320	2.546784e-48	3.468298e-46
MsaG008547	MsaG027467	0.812294	7.835164e-51	1.415302e-48
MsaG008547	MsaG027590	0.808206	5.908344e-50	9.662732e-48
MsaG008547	MsaG027861	0.817855	4.625371e-52	9.618265e-50
MsaG008547	MsaG028619	0.809525	3.095060e-50	5.225351e-48
MsaG008547	MsaG029464	0.825064	1.019298e-53	2.568637e-51
MsaG008547	MsaG031007	0.805185	2.549445e-49	3.881792e-47
MsaG008547	MsaG031781	0.815216	1.792227e-51	3.483585e-49
MsaG008547	MsaG032970	0.816788	8.021587e-52	1.622817e-49
MsaG008547	MsaG042511	0.800306	2.563534e-48	3.489997e-46
MsaG008547	MsaG044546	0.830691	4.585258e-55	1.353429e-52
MsaG008547	MsaG012667	0.822575	3.880443e-53	9.139067e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG008547	MtrunA17_Chr2g0304031	74.667	225	36	5	131	349	10	219	2.95e-101	298
MsaG008547	MtrunA17_Chr2g0303981	79.130	115	22	1	1	115	1	113	7.31e-56	181
MsaG008547	MtrunA17_Chr4g0059631	28.857	350	138	12	1	335	1	254	8.10e-29	112
MsaG008547	MtrunA17_Chr1g0212471	47.368	133	63	3	4	133	2	130	1.56e-28	110
MsaG008547	MtrunA17_Chr4g0022641	31.534	352	159	13	4	346	2	280	2.62e-27	111
MsaG008547	MtrunA17_Chr6g0449851	37.190	121	68	3	4	120	2	118	1.67e-16	79.3
MsaG008547	MtrunA17_Chr1g0212151	36.364	121	69	3	4	120	2	118	2.91e-16	79.0
MsaG008547	MtrunA17_Chr6g0457851	37.190	121	68	3	4	120	12	128	4.03e-16	79.0
MsaG008547	MtrunA17_Chr6g0449861	33.884	121	72	3	4	120	10	126	2.56e-14	73.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCACTGGCACTAACCAATT+TGG	0.223800	2:+37050842	MsaT008547.1:CDS
ACTTCACAAATTCACCAAAT+TGG	0.235452	2:-37050856	None:intergenic
CAATCAGAGAAACAATAATT+TGG	0.236694	2:+37050251	MsaT008547.1:CDS
ATGAGTAACAATCCAGATTT+TGG	0.240718	2:+37051436	MsaT008547.1:CDS
CATCATGCATGCCCTCAAAT+TGG	0.311938	2:-37051102	None:intergenic
TGGTGAAGGTGTGGAGATTA+AGG	0.327636	2:+37051204	MsaT008547.1:CDS
CACAATCTCTTTGTGCACTT+TGG	0.338528	2:-37050505	None:intergenic
TGTTGCACATCAACATGATT+AGG	0.350397	2:-37050749	None:intergenic
TTGTCAGTACGGCCAAAATC+TGG	0.361644	2:-37051448	None:intergenic
GAATGGCTTGCTAAACTTGA+TGG	0.361953	2:+37050968	MsaT008547.1:CDS
AAAACATGAGGCAAACTAAC+AGG	0.362572	2:-37050818	None:intergenic
CTTGCTAAACTTGATGGAAT+TGG	0.393389	2:+37050974	MsaT008547.1:CDS
TTAGCAAGCCATTCTTGAAC+AGG	0.402628	2:-37050959	None:intergenic
GAAACAGGAGGCAAACTAAC+AGG	0.403384	2:-37051166	None:intergenic
TTGATTGTATATGATGGTTA+TGG	0.415733	2:+37050321	MsaT008547.1:CDS
TTCAAATTCAAACCAATTTG+AGG	0.418562	2:+37051090	MsaT008547.1:CDS
GAATCTTGTGGCGTAGTCAT+TGG	0.433592	2:-37050354	None:intergenic
TGAAGAACAATATGCAACTT+TGG	0.433757	2:+37051405	MsaT008547.1:CDS
TGTGAAGTTGGATGATGAAA+TGG	0.438732	2:+37050871	MsaT008547.1:CDS
ATTTGGTGAATTTGTGAAGT+TGG	0.438819	2:+37050859	MsaT008547.1:CDS
ACTTGATGGAATTGGTGAAA+TGG	0.440564	2:+37050982	MsaT008547.1:CDS
CACTTTGGAAATCTCGGCTT+CGG	0.449137	2:-37050490	None:intergenic
AGTTGGTTAGTCGACGAAAC+AGG	0.451962	2:-37051181	None:intergenic
TCAAATTCAAACCAATTTGA+GGG	0.460892	2:+37051091	MsaT008547.1:CDS
TCACCATCATCTAGTTTACT+AGG	0.464092	2:-37051277	None:intergenic
TTTGTGCACTTTGGAAATCT+CGG	0.467282	2:-37050496	None:intergenic
TGGGCAAACCTGTTCAAGAA+TGG	0.474989	2:+37050951	MsaT008547.1:CDS
TCATGTTGATGTGCAACAAA+GGG	0.515172	2:+37051015	MsaT008547.1:CDS
TACAAAGATCTTTGATGTGA+AGG	0.515872	2:+37050440	MsaT008547.1:CDS
CAATTTGAGGGCATGCATGA+TGG	0.516426	2:+37051103	MsaT008547.1:CDS
ACCAAATTGGTTAGTGCCAG+TGG	0.517469	2:-37050843	None:intergenic
AAATGGCTGAACCTAGAGAA+TGG	0.526305	2:+37050888	MsaT008547.1:CDS
TATGAGCAACAAAAGATTGA+GGG	0.532792	2:+37050405	MsaT008547.1:CDS
GCTTGCTTGATTGTATATGA+TGG	0.536036	2:+37050315	MsaT008547.1:CDS
GACTTCAAAGGATCATAGAT+AGG	0.536260	2:-37051142	None:intergenic
TGGAGATTAAGGATCAAGAA+TGG	0.537647	2:+37051215	MsaT008547.1:CDS
ATATGAGCAACAAAAGATTG+AGG	0.539428	2:+37050404	MsaT008547.1:CDS
TCTCCACACCTTCACCAAGT+TGG	0.544036	2:-37051198	None:intergenic
ATCATGTTGATGTGCAACAA+AGG	0.544586	2:+37051014	MsaT008547.1:CDS
AGAGAATGGACTGGTCAACT+TGG	0.545153	2:+37050902	MsaT008547.1:CDS
CACTGCTCACACACAACACA+TGG	0.545176	2:+37051057	MsaT008547.1:CDS
AGTTGACCAGTCCATTCTCT+AGG	0.550305	2:-37050899	None:intergenic
ACAATATGCAACTTTGGAGG+AGG	0.551423	2:+37051411	MsaT008547.1:CDS
GCAGAAATGGGTCGTGCTCG+TGG	0.560131	2:+37050177	None:intergenic
GCTCCTAGTAAACTAGATGA+TGG	0.572128	2:+37051274	MsaT008547.1:CDS
GTCAAGATGATGTGTTTGCA+TGG	0.582853	2:+37051356	MsaT008547.1:CDS
GTATATGATGGTTATGGTGA+TGG	0.584410	2:+37050327	MsaT008547.1:CDS
AATATGCAACTTTGGAGGAG+GGG	0.586542	2:+37051413	MsaT008547.1:CDS
AGAACAATATGCAACTTTGG+AGG	0.594299	2:+37051408	MsaT008547.1:CDS
CAATATGCAACTTTGGAGGA+GGG	0.594606	2:+37051412	MsaT008547.1:CDS
AAACTAACAGGGTAGTTCAA+AGG	0.601389	2:-37050806	None:intergenic
ACATAGAAGATGATAGAGCT+CGG	0.603472	2:+37050217	MsaT008547.1:CDS
GTTTCGTCGACTAACCAACT+TGG	0.615554	2:+37051184	MsaT008547.1:CDS
CACAAGATTCGACAATTGTA+CGG	0.618314	2:+37050367	MsaT008547.1:CDS
TAACCAACTTGGTGAAGGTG+TGG	0.620796	2:+37051195	MsaT008547.1:CDS
CGTACAATTGTCGAATCTTG+TGG	0.622061	2:-37050366	None:intergenic
GCTGAACCTAGAGAATGGAC+TGG	0.629743	2:+37050893	MsaT008547.1:CDS
TCGACTAACCAACTTGGTGA+AGG	0.633986	2:+37051190	MsaT008547.1:CDS
AAACTAACAGGAGACTTCAA+AGG	0.635174	2:-37051154	None:intergenic
TGGTCAACTTGGTGAACATG+AGG	0.636156	2:+37050913	MsaT008547.1:CDS
AAGTGGAATAGTCAAGATGA+TGG	0.637862	2:+37051310	MsaT008547.1:CDS
AAACATGAGGCAAACTAACA+GGG	0.648457	2:-37050817	None:intergenic
ATGGTGATGATATTATGAAG+TGG	0.662410	2:+37051293	MsaT008547.1:CDS
GTGCCAGTGGACAAAACATG+AGG	0.672995	2:-37050830	None:intergenic
TGGTTAGTCGACGAAACAGG+AGG	0.703043	2:-37051178	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	37050183	37051486	37050183	ID=MsaG008547
Chr2	mRNA	37050183	37051486	37050183	ID=MsaT008547.1;Parent=MsaG008547
Chr2	exon	37050183	37050535	37050183	ID=MsaT008547.1.exon1;Parent=MsaT008547.1
Chr2	CDS	37050183	37050535	37050183	ID=cds.MsaT008547.1;Parent=MsaT008547.1
Chr2	exon	37050739	37051486	37050739	ID=MsaT008547.1.exon2;Parent=MsaT008547.1
Chr2	CDS	37050739	37051486	37050739	ID=cds.MsaT008547.1;Parent=MsaT008547.1

Gene Sequence

>MsaT008547.1

ATGGGTCGTGCTCGTGGAAAGTTAACTATTAAACACATAGAAGATGATAGAGCTCGGAAATTAGCTTTCAATCAGAGAAACAATAATTTGGCAAAAAAAGTTTCTGATTTTTCTAAAAAGTTTGGAGTTGAAGCTTGCTTGATTGTATATGATGGTTATGGTGATGGTAGACCAATGACTACGCCACAAGATTCGACAATTGTACGGTCCATGCTTGAAAAATATGAGCAACAAAAGATTGAGGGTACAAGTACAACTACAAAGATCTTTGATGTGAAGGACTATTTTGCAAACAAGATAAAGAAGGCCGAAGCCGAGATTTCCAAAGTGCACAAAGAGATTGTGATGAAAATGCAATATATACCTAATCATGTTGATGTGCAACAAAGTGAAACTAGTTTCACACAAAACTCTGCTGATCCTTTGAACTACCCTGTTAGTTTGCCTCATGTTTTGTCCACTGGCACTAACCAATTTGGTGAATTTGTGAAGTTGGATGATGAAATGGCTGAACCTAGAGAATGGACTGGTCAACTTGGTGAACATGAGGAGTTGAATAATTTTGTGGGCAAACCTGTTCAAGAATGGCTTGCTAAACTTGATGGAATTGGTGAAATGGATTTCACTAATCATGTTGATGTGCAACAAAGGGAAACTAGTTTCACACAAAACACTGCTCACACACAACACATGGCTAATGACGCTTCAAATTCAAACCAATTTGAGGGCATGCATGATGGCTTTTATCAATGGCAACCTATCTATGATCCTTTGAAGTCTCCTGTTAGTTTGCCTCCTGTTTCGTCGACTAACCAACTTGGTGAAGGTGTGGAGATTAAGGATCAAGAATGGTTTACTCATACTGAACTTCTCAATGTTGATGATCAAGCTCCTAGTAAACTAGATGATGGTGATGATATTATGAAGTGGAATAGTCAAGATGATGGTGATGAACTTATAAAGTTGATTAGTCAAGATGATGTGTTTGCATGGAAAGATGACTCTTCTGTGTCTCAAAATGAAGAACAATATGCAACTTTGGAGGAGGGGATGAGTAACAATCCAGATTTTGGCCGTACTGACAAAACTCTCTTCCAGTGA

Protein sequence

>MsaT008547.1

MGRARGKLTIKHIEDDRARKLAFNQRNNNLAKKVSDFSKKFGVEACLIVYDGYGDGRPMTTPQDSTIVRSMLEKYEQQKIEGTSTTTKIFDVKDYFANKIKKAEAEISKVHKEIVMKMQYIPNHVDVQQSETSFTQNSADPLNYPVSLPHVLSTGTNQFGEFVKLDDEMAEPREWTGQLGEHEELNNFVGKPVQEWLAKLDGIGEMDFTNHVDVQQRETSFTQNTAHTQHMANDASNSNQFEGMHDGFYQWQPIYDPLKSPVSLPPVSSTNQLGEGVEIKDQEWFTHTELLNVDDQAPSKLDDGDDIMKWNSQDDGDELIKLISQDDVFAWKDDSSVSQNEEQYATLEEGMSNNPDFGRTDKTLFQ*