AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008587

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008587 MtrunA17_Chr4g0068031 74.757 103 20 2 34 130 16 118 1.02e-37 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008587 AT3G06740.1 47.778 90 35 3 44 130 37 117 6.16e-16 69.7
MsaG008587 AT4G16141.1 63.265 49 15 1 45 90 34 82 4.27e-13 63.5
MsaG008587 AT5G49300.1 46.429 84 35 2 44 124 32 108 9.71e-13 61.2
MsaG008587 AT3G16870.1 46.753 77 28 3 45 118 39 105 1.18e-11 59.3

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAAGATTATTGAATTTA+AGG 0.060866 2:+37967415 MsaT008587.1:CDS
AAGAAGAAGAGAGCAATATT+AGG 0.216353 2:+37967300 MsaT008587.1:CDS
AACAGAGCTAGTAAAGTTTA+TGG 0.220023 2:-37967065 None:intergenic
ACCACGACATTGAGGAGTTT+TGG 0.295427 2:-37967137 None:intergenic
TCTCTTCTTCTTCCTGCTCC+TGG 0.356990 2:-37967289 None:intergenic
AGAAGAAGAGAGCAATATTA+GGG 0.387155 2:+37967301 MsaT008587.1:CDS
AACGAAGAAGGAACAAGAAA+GGG 0.398579 2:+37967339 MsaT008587.1:CDS
AGAGAGCAATATTAGGGATA+AGG 0.418283 2:+37967307 MsaT008587.1:CDS
AATGTAAGTGGAAGCAAAGT+TGG 0.422122 2:+37967372 MsaT008587.1:CDS
ATGTAAGTGGAAGCAAAGTT+GGG 0.426431 2:+37967373 MsaT008587.1:CDS
ATGCTTGCGGGACCAGGAGC+AGG 0.445349 2:+37967277 MsaT008587.1:CDS
CAACGAAGAAGGAACAAGAA+AGG 0.448446 2:+37967338 MsaT008587.1:CDS
TGTGCAATGCTTGCGGGACC+AGG 0.480073 2:+37967271 MsaT008587.1:CDS
GGACGTTCCGCAACCGGCAC+AGG 0.481338 2:-37967116 None:intergenic
AAGAAAACCTGTGCCGGTTG+CGG 0.485045 2:+37967109 MsaT008587.1:CDS
AGTCACTGTGCAATGCTTGC+GGG 0.493499 2:+37967265 MsaT008587.1:intron
GGGAATAAGAGTAATGTAAG+TGG 0.508753 2:+37967360 MsaT008587.1:CDS
AGGAAAGGAAACAACGAAGA+AGG 0.523485 2:+37967327 MsaT008587.1:CDS
TTGAGTAATGCTATATGGTA+CGG 0.528195 2:+37966002 None:intergenic
AATCCAAAGAAAACCTGTGC+CGG 0.567940 2:+37967103 MsaT008587.1:CDS
GCAATATTAGGGATAAGGAA+AGG 0.573561 2:+37967312 MsaT008587.1:CDS
CAGTCACTGTGCAATGCTTG+CGG 0.581160 2:+37967264 MsaT008587.1:intron
TTTGGACCACCACGACATTG+AGG 0.595285 2:-37967145 None:intergenic
TCAATGTCGTGGTGGTCCAA+AGG 0.602676 2:+37967147 MsaT008587.1:CDS
TGTAAGTGGAAGCAAAGTTG+GGG 0.606130 2:+37967374 MsaT008587.1:CDS
TCCAAAACTCCTCAATGTCG+TGG 0.628200 2:+37967136 MsaT008587.1:CDS
ACGAAGAAGGAACAAGAAAG+GGG 0.636309 2:+37967340 MsaT008587.1:CDS
AAAACTCCTCAATGTCGTGG+TGG 0.684546 2:+37967139 MsaT008587.1:CDS
GTAAGTGGAAGCAAAGTTGG+GGG 0.706993 2:+37967375 MsaT008587.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 37966009 37967461 37966009 ID=MsaG008587
Chr2 mRNA 37966009 37967461 37966009 ID=MsaT008587.1;Parent=MsaG008587
Chr2 exon 37966009 37966092 37966009 ID=MsaT008587.1.exon1;Parent=MsaT008587.1
Chr2 CDS 37966009 37966092 37966009 ID=cds.MsaT008587.1;Parent=MsaT008587.1
Chr2 exon 37967055 37967168 37967055 ID=MsaT008587.1.exon2;Parent=MsaT008587.1
Chr2 CDS 37967055 37967168 37967055 ID=cds.MsaT008587.1;Parent=MsaT008587.1
Chr2 exon 37967267 37967461 37967267 ID=MsaT008587.1.exon3;Parent=MsaT008587.1
Chr2 CDS 37967267 37967461 37967267 ID=cds.MsaT008587.1;Parent=MsaT008587.1
Gene Sequence

>MsaT008587.1

ATGCTATATGGTACGGAACATGCATGTACGAAAATCAACGAATGTGAAATTGAAGCATTTAAGTTGCATTGTTTCAATAGAAAAATCTTGCATGCCATAAACTTTACTAGCTCTGTTTCATCAGATAACAACAATCCAAAGAAAACCTGTGCCGGTTGCGGAACGTCCAAAACTCCTCAATGTCGTGGTGGTCCAAAGTCACTGTGCAATGCTTGCGGGACCAGGAGCAGGAAGAAGAAGAGAGCAATATTAGGGATAAGGAAAGGAAACAACGAAGAAGGAACAAGAAAGGGGAATAAGAGTAATGTAAGTGGAAGCAAAGTTGGGGGAAGAAATTTGAACATGAATCAAAGATTATTGAATTTAAGGAAAGAAGTGTTTATGAATCGATAA
Protein sequence

>MsaT008587.1

MLYGTEHACTKINECEIEAFKLHCFNRKILHAINFTSSVSSDNNNPKKTCAGCGTSKTPQCRGGPKSLCNACGTRSRKKKRAILGIRKGNNEEGTRKGNKSNVSGSKVGGRNLNMNQRLLNLRKEVFMNR*