AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008889


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008889 MtrunA17_Chr2g0307131 91.086 359 27 2 1 358 1 355 0.0 630
MsaG008889 MtrunA17_Chr2g0307141 88.187 364 34 4 1 359 1 360 0.0 598
MsaG008889 MtrunA17_Chr6g0468491 70.294 340 72 5 23 359 1 314 4.31e-161 453
MsaG008889 MtrunA17_Chr4g0033391 66.017 359 80 7 1 357 1 319 2.88e-149 424
MsaG008889 MtrunA17_Chr6g0468461 63.977 347 89 5 1 342 1 316 2.07e-141 405
MsaG008889 MtrunA17_Chr4g0033291 63.733 375 74 8 1 359 1 329 1.05e-138 397
MsaG008889 MtrunA17_Chr4g0033281 63.271 373 74 6 1 358 1 325 1.19e-136 392
MsaG008889 MtrunA17_Chr4g0054581 60.309 388 116 9 1 359 1 379 2.43e-136 393
MsaG008889 MtrunA17_Chr4g0054621 51.491 369 142 10 1 359 1 342 8.47e-108 319
MsaG008889 MtrunA17_Chr7g0225901 61.417 254 66 4 86 334 14 240 1.88e-97 289
MsaG008889 MtrunA17_Chr5g0400621 41.047 363 147 8 18 358 16 333 1.27e-61 200
MsaG008889 MtrunA17_Chr5g0400641 56.250 160 62 2 185 340 1 156 1.40e-48 161
MsaG008889 MtrunA17_Chr2g0280161 44.792 192 105 1 167 357 162 353 4.12e-48 166
MsaG008889 MtrunA17_Chr5g0400661 34.444 360 184 9 10 350 4 330 3.85e-46 160
MsaG008889 MtrunA17_Chr4g0002801 38.247 251 134 4 112 355 95 331 7.62e-45 157
MsaG008889 MtrunA17_Chr4g0054551 46.199 171 88 2 180 349 153 320 1.84e-43 153
MsaG008889 MtrunA17_Chr4g0054561 47.368 171 86 2 180 349 153 320 4.13e-43 152
MsaG008889 MtrunA17_Chr4g0002781 38.554 249 136 4 114 355 112 350 1.43e-41 149
MsaG008889 MtrunA17_Chr2g0333621 37.097 248 136 6 126 358 73 315 6.58e-41 146
MsaG008889 MtrunA17_Chr2g0333641 38.136 236 130 6 127 349 70 302 8.69e-41 145
MsaG008889 MtrunA17_Chr4g0049331 42.458 179 103 0 172 350 125 303 2.79e-40 144
MsaG008889 MtrunA17_Chr8g0336681 33.117 308 163 8 84 355 36 336 1.81e-39 143
MsaG008889 MtrunA17_Chr4g0054571 43.721 215 95 5 158 350 115 325 1.11e-38 140
MsaG008889 MtrunA17_Chr5g0400671 35.156 256 138 4 114 351 75 320 1.74e-37 137
MsaG008889 MtrunA17_Chr2g0333541 35.821 268 155 5 100 353 3 267 2.23e-35 130
MsaG008889 MtrunA17_Chr7g0249811 40.000 185 102 1 171 355 154 329 5.03e-35 130
MsaG008889 MtrunA17_Chr2g0333611 33.945 218 140 3 140 356 3 217 1.80e-34 126
MsaG008889 MtrunA17_Chr4g0002851 37.255 153 64 2 205 350 65 192 8.28e-23 94.7
MsaG008889 MtrunA17_Chr7g0233441 37.671 146 81 2 205 350 57 192 2.61e-22 93.2
MsaG008889 MtrunA17_Chr4g0002831 35.928 167 79 5 193 350 53 200 1.13e-21 91.7
MsaG008889 MtrunA17_Chr1g0185621 30.270 185 112 5 143 315 437 616 2.83e-18 86.3
MsaG008889 MtrunA17_Chr8g0366751 33.951 162 93 3 171 318 448 609 8.40e-17 81.6
MsaG008889 MtrunA17_Chr8g0346161 30.496 141 91 2 182 318 291 428 9.73e-16 78.2
MsaG008889 MtrunA17_Chr5g0411341 31.844 179 80 6 168 318 476 640 7.78e-15 75.9
MsaG008889 MtrunA17_Chr8g0365711 27.027 185 108 4 161 331 227 398 7.87e-14 72.4
MsaG008889 MtrunA17_Chr7g0275821 31.056 161 99 3 179 335 245 397 8.68e-14 72.0
MsaG008889 MtrunA17_Chr7g0260841 43.333 90 42 2 155 235 319 408 4.61e-13 70.1
MsaG008889 MtrunA17_Chr8g0346151 30.625 160 97 4 139 291 240 392 2.36e-12 67.8
MsaG008889 MtrunA17_Chr1g0211081 26.606 218 128 6 133 318 264 481 1.35e-11 65.9
MsaG008889 MtrunA17_Chr8g0386921 40.278 72 43 0 174 245 398 469 8.37e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008889 AT4G37850.2 40.359 223 122 4 139 357 113 328 2.44e-41 147
MsaG008889 AT4G37850.1 40.359 223 122 4 139 357 113 328 2.44e-41 147
MsaG008889 AT2G22760.1 33.677 291 154 7 86 350 14 291 1.68e-37 137
MsaG008889 AT2G22750.3 44.974 189 98 2 166 349 138 325 2.72e-36 134
MsaG008889 AT2G22750.2 43.386 189 101 2 166 349 110 297 2.76e-36 134
MsaG008889 AT2G22750.5 44.974 189 98 2 166 349 134 321 3.74e-36 134
MsaG008889 AT2G22750.1 44.974 189 97 3 166 349 110 296 5.07e-35 130
MsaG008889 AT2G22750.4 44.974 189 97 3 166 349 138 324 5.77e-35 131
MsaG008889 AT2G22760.2 33.212 274 145 6 86 334 14 274 5.60e-33 124
MsaG008889 AT2G22770.1 33.077 260 120 8 120 350 81 315 2.95e-31 120
MsaG008889 AT5G46760.1 32.394 213 111 8 151 338 388 592 6.92e-19 88.6
MsaG008889 AT4G17880.1 36.420 162 85 5 168 315 399 556 8.70e-18 85.1
MsaG008889 AT1G32640.1 32.692 156 93 4 171 318 438 589 8.41e-16 79.3
MsaG008889 AT1G01260.1 28.485 165 92 3 171 331 418 560 3.58e-14 74.3
MsaG008889 AT1G01260.2 28.485 165 92 3 171 331 418 560 3.58e-14 74.3
MsaG008889 AT1G01260.3 28.485 165 92 3 171 331 418 560 3.58e-14 74.3
MsaG008889 AT1G63650.3 30.409 171 92 5 182 330 405 570 8.45e-14 73.2
MsaG008889 AT1G63650.1 30.409 171 92 5 182 330 405 570 8.45e-14 73.2
MsaG008889 AT1G63650.2 30.409 171 92 5 182 330 405 570 8.45e-14 73.2
MsaG008889 AT2G46510.1 37.391 115 58 4 142 244 343 455 1.00e-13 72.8
MsaG008889 AT4G09820.1 30.682 176 86 6 183 351 364 510 2.24e-13 71.6
MsaG008889 AT4G16430.1 46.154 78 39 1 168 242 303 380 2.30e-13 71.2
MsaG008889 AT5G41315.3 30.851 188 97 8 170 330 435 616 3.05e-12 68.2
MsaG008889 AT5G41315.2 30.851 188 97 8 170 330 438 619 3.50e-12 68.2
MsaG008889 AT5G41315.1 30.851 188 97 8 170 330 430 611 3.54e-12 68.2
MsaG008889 AT4G00870.1 33.058 121 66 2 183 294 250 364 1.64e-11 65.5

Find 52 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTAGTAGTAGGTGAATT+TGG 0.205689 2:+48861512 None:intergenic
CAGCAGCTGGTTATCTGATT+TGG 0.298434 2:-48863235 MsaT008889.1:intron
CGATATTGATAAGACAATTA+TGG 0.305371 2:-48861633 MsaT008889.1:CDS
TCTAGTTCCCAACCTTAAAA+AGG 0.316998 2:-48861285 MsaT008889.1:intron
TTCAGTGTCTCCAACTGAAT+TGG 0.327767 2:-48861435 MsaT008889.1:CDS
CACCAGTATCAAGGAAATTC+AGG 0.328339 2:+48861792 None:intergenic
GGGACTTCTAGTTAAAATTA+TGG 0.355331 2:-48860581 MsaT008889.1:CDS
CTGCAAGAGCAATGAAGCTT+TGG 0.357341 2:+48861309 None:intergenic
AAACAACAATGTTTAACTTC+GGG 0.367096 2:-48861719 MsaT008889.1:CDS
CAAGTGGAAGCAAAAGTGTT+GGG 0.403623 2:-48860643 MsaT008889.1:CDS
AACCTGAATTTCCTTGATAC+TGG 0.413104 2:-48861794 MsaT008889.1:CDS
AAAAGGTCTCGTGCTAATAA+TGG 0.413380 2:-48861377 MsaT008889.1:CDS
CAAAAGATGGGATTTGAACT+TGG 0.429625 2:+48861543 None:intergenic
GGTCAAATCCAGAAAATTGT+TGG 0.450466 2:+48861489 None:intergenic
TGGGGAAATATATGTTGATT+CGG 0.457282 2:-48860624 MsaT008889.1:CDS
TAAACAACAATGTTTAACTT+CGG 0.458515 2:-48861720 MsaT008889.1:CDS
ATTTAGTGATGAAACAAAGT+TGG 0.458588 2:-48861669 MsaT008889.1:CDS
TGCAAGAGCAATGAAGCTTT+GGG 0.460124 2:+48861310 None:intergenic
ATCACTGCAGAGGTCTGGTT+TGG 0.472005 2:+48860720 None:intergenic
TGATATTACTATCATTGCTC+AGG 0.480526 2:-48860491 MsaT008889.1:intron
GTCAAATCCAGAAAATTGTT+GGG 0.480993 2:+48861490 None:intergenic
TTTGGAGGGCTGTCAAAAGA+TGG 0.482718 2:+48861530 None:intergenic
TCATCATCACTGCAGAGGTC+TGG 0.484137 2:+48860715 None:intergenic
GAGCGCAATGAAAGTGTTGT+TGG 0.490652 2:-48860676 MsaT008889.1:CDS
GGATCCAATGTAAGGATCAC+AGG 0.493804 2:-48860603 MsaT008889.1:CDS
AATTGTTGGGAACTAGTAGT+AGG 0.495232 2:+48861503 None:intergenic
GTTGATTCGGATCCAATGTA+AGG 0.498122 2:-48860611 MsaT008889.1:CDS
AGGCTATAACTTGAGCATAA+AGG 0.504535 2:-48860247 MsaT008889.1:CDS
AATCTTCCACACCAGTATCA+AGG 0.520277 2:+48861783 None:intergenic
GAGCAATGATAGTAATATCA+AGG 0.522227 2:+48860494 None:intergenic
GGAACAGAATTTATTTGCTG+AGG 0.534779 2:-48861822 MsaT008889.1:CDS
TTGGAGGGCTGTCAAAAGAT+GGG 0.546086 2:+48861531 None:intergenic
CTAGTAGTAGGTGAATTTGG+AGG 0.546361 2:+48861515 None:intergenic
TCAAGTGGAAGCAAAAGTGT+TGG 0.548818 2:-48860644 MsaT008889.1:CDS
ACGTATCATCAAGCAGCAGC+TGG 0.560203 2:-48863248 MsaT008889.1:CDS
GTCCCCTGTGATCCTTACAT+TGG 0.562355 2:+48860599 None:intergenic
TCAAGGATGGAATCTCCAAA+GGG 0.562816 2:+48860511 None:intergenic
TAATGGTGAGGATCATATCA+TGG 0.568755 2:-48861360 MsaT008889.1:CDS
TAGTAGTAGGTGAATTTGGA+GGG 0.573251 2:+48861516 None:intergenic
ATCAAGGATGGAATCTCCAA+AGG 0.580088 2:+48860510 None:intergenic
AATGATAGTAATATCAAGGA+TGG 0.583388 2:+48860498 None:intergenic
AACAACAATGTTTAACTTCG+GGG 0.586447 2:-48861718 MsaT008889.1:CDS
AAGTGGAAGCAAAAGTGTTG+GGG 0.597956 2:-48860642 MsaT008889.1:CDS
ATTGCGCTCATCAAATGATG+AGG 0.605739 2:+48860690 None:intergenic
TGTTGGTTCAATATTTCAAG+TGG 0.607961 2:-48860659 MsaT008889.1:CDS
GTCTCGTGCTAATAATGGTG+AGG 0.616632 2:-48861372 MsaT008889.1:CDS
GAATTTCCTTGATACTGGTG+TGG 0.624759 2:-48861789 MsaT008889.1:CDS
GTTGCTGAGAGTTGTGCAAG+TGG 0.638379 2:+48861694 None:intergenic
GATCCAATGTAAGGATCACA+GGG 0.640120 2:-48860602 MsaT008889.1:CDS
AGGAATCATCATCACTGCAG+AGG 0.647357 2:+48860710 None:intergenic
GCTTGTGAAGAACATACGCA+AGG 0.669181 2:-48860223 MsaT008889.1:CDS
ATCCAATGTAAGGATCACAG+GGG 0.825165 2:-48860601 MsaT008889.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 48860197 48863298 48860197 ID=MsaG008889
Chr2 mRNA 48860197 48863298 48860197 ID=MsaT008889.1;Parent=MsaG008889
Chr2 exon 48860197 48860277 48860197 ID=MsaT008889.1.exon4;Parent=MsaT008889.1
Chr2 CDS 48860197 48860277 48860197 ID=cds.MsaT008889.1;Parent=MsaT008889.1
Chr2 exon 48860492 48860860 48860492 ID=MsaT008889.1.exon3;Parent=MsaT008889.1
Chr2 CDS 48860492 48860860 48860492 ID=cds.MsaT008889.1;Parent=MsaT008889.1
Chr2 exon 48861286 48861852 48861286 ID=MsaT008889.1.exon2;Parent=MsaT008889.1
Chr2 CDS 48861286 48861852 48861286 ID=cds.MsaT008889.1;Parent=MsaT008889.1
Chr2 exon 48863236 48863298 48863236 ID=MsaT008889.1.exon1;Parent=MsaT008889.1
Chr2 CDS 48863236 48863298 48863236 ID=cds.MsaT008889.1;Parent=MsaT008889.1
Gene Sequence

>MsaT008889.1

ATGGAGGAAATCAACAACACAGCTATGAACGTATCATCAAGCAGCAGCTGGTTATCTGATTTGGAAATGGAGGAACAGAATTTATTTGCTGAGGAATGCAACCTGAATTTCCTTGATACTGGTGTGGAAGATTTTCTTTCACATGACATAGCTAATGTCTTCCAAGAACAAAATAAACAACAATGTTTAACTTCGGGGTCCACTTGCACAACTCTCAGCAACACATTTAGTGATGAAACAAAGTTGGATTGTTTTGACTTCGATATTGATAAGACAATTATGGAGATGAAAACTATTGATCATAGTGACAAAATCAATGAAACCTTTGCACAAAAACTTTCTTCATCTTTCCAAGTTCAAATCCCATCTTTTGACAGCCCTCCAAATTCACCTACTACTAGTTCCCAACAATTTTCTGGATTTGACCCTACCTTAAACTCAAAACAAAATGAAACAGTTTCAGTGTCTCCAACTGAATTGGAAAATAAGAATAATTCAACAAAAACCTTAAAAAGCAAAAGGTCTCGTGCTAATAATGGTGAGGATCATATCATGGCAGAGAGAAAGCGAAGAGAGAAACTCACCCAAAGCTTCATTGCTCTTGCAGCTCTAGTTCCCAACCTTAAAAAGATGGACAAGTTTTCTGTATTAGTGGACACTATCAAATACATGAAAGAGCTTAAAAAGCGTTTGGAAGTTTTAGAAGAACAAAACAAGAGAATAAAAACAGAGTCACATGTGGTTCTGACCAAACCAGACCTCTGCAGTGATGATGATTCCTCATCATTTGATGAGCGCAATGAAAGTGTTGTTGGTTCAATATTTCAAGTGGAAGCAAAAGTGTTGGGGAAATATATGTTGATTCGGATCCAATGTAAGGATCACAGGGGACTTCTAGTTAAAATTATGGTCGAGATTCAAAGATTTCAACTATGTGTTGTCAATAGTAGTGTTTTACCCTTTGGAGATTCCATCCTTGATATTACTATCATTGCTCAGTTGGGTGAAGGCTATAACTTGAGCATAAAGGAGCTTGTGAAGAACATACGCAAGGAAGCATTGAAGTTTATGTCATCATAA

Protein sequence

>MsaT008889.1

MEEINNTAMNVSSSSSWLSDLEMEEQNLFAEECNLNFLDTGVEDFLSHDIANVFQEQNKQQCLTSGSTCTTLSNTFSDETKLDCFDFDIDKTIMEMKTIDHSDKINETFAQKLSSSFQVQIPSFDSPPNSPTTSSQQFSGFDPTLNSKQNETVSVSPTELENKNNSTKTLKSKRSRANNGEDHIMAERKRREKLTQSFIALAALVPNLKKMDKFSVLVDTIKYMKELKKRLEVLEEQNKRIKTESHVVLTKPDLCSDDDSSSFDERNESVVGSIFQVEAKVLGKYMLIRIQCKDHRGLLVKIMVEIQRFQLCVVNSSVLPFGDSILDITIIAQLGEGYNLSIKELVKNIRKEALKFMSS*