AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008911

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008911 MtrunA17_Chr2g0307551 99.359 156 1 0 54 209 1 156 1.52e-114 323
MsaG008911 MtrunA17_Chr4g0062271 84.967 153 23 0 54 206 1 153 7.73e-97 278
MsaG008911 MtrunA17_Chr1g0185911 31.967 122 82 1 47 167 11 132 3.23e-19 81.3
MsaG008911 MtrunA17_Chr4g0076381 37.500 96 58 1 49 144 45 138 2.94e-17 77.4
MsaG008911 MtrunA17_Chr7g0263601 32.432 111 73 1 65 173 27 137 5.74e-17 75.1
MsaG008911 MtrunA17_Chr1g0195851 37.179 78 49 0 65 142 37 114 1.05e-16 75.1
MsaG008911 MtrunA17_Chr1g0191981 35.443 79 51 0 66 144 6 84 8.28e-16 70.9
MsaG008911 MtrunA17_Chr1g0165041 33.333 81 54 0 64 144 6 86 1.47e-15 71.6
MsaG008911 MtrunA17_Chr3g0102351 37.179 78 49 0 65 142 28 105 1.90e-15 71.6
MsaG008911 MtrunA17_Chr5g0446491 34.118 85 56 0 60 144 19 103 4.92e-15 70.1
MsaG008911 MtrunA17_Chr8g0384451 30.088 113 73 1 65 177 41 147 7.55e-15 70.5
MsaG008911 MtrunA17_Chr4g0067091 36.709 79 50 0 64 142 22 100 8.95e-15 70.1
MsaG008911 MtrunA17_Chr2g0296321 33.333 105 64 1 65 163 67 171 3.80e-14 69.3
MsaG008911 MtrunA17_Chr2g0290491 33.333 78 52 0 65 142 18 95 8.85e-14 66.6
MsaG008911 MtrunA17_Chr8g0382931 27.119 118 80 1 58 175 22 133 1.06e-13 67.0
MsaG008911 MtrunA17_Chr4g0076321 35.135 74 48 0 69 142 24 97 9.89e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008911 AT5G23090.5 79.870 154 29 1 54 205 1 154 3.48e-88 257
MsaG008911 AT5G23090.2 79.870 154 29 1 54 205 1 154 3.48e-88 257
MsaG008911 AT5G23090.1 79.870 154 29 1 54 205 1 154 3.48e-88 257
MsaG008911 AT5G23090.4 79.221 154 29 2 54 205 1 153 1.48e-85 250
MsaG008911 AT5G08190.1 83.803 142 23 0 54 195 1 142 8.03e-79 233
MsaG008911 AT5G08190.2 83.099 142 23 1 54 195 1 141 3.52e-76 226
MsaG008911 AT5G23090.3 71.429 154 29 2 54 205 1 141 3.82e-74 221
MsaG008911 AT2G38880.11 35.484 93 58 1 65 155 22 114 1.22e-17 77.0
MsaG008911 AT2G38880.8 35.484 93 58 1 65 155 22 114 1.22e-17 77.0
MsaG008911 AT2G38880.7 36.471 85 52 1 65 147 22 106 2.49e-17 75.5
MsaG008911 AT2G38880.4 36.471 85 52 1 65 147 22 106 2.52e-17 74.7
MsaG008911 AT2G38880.2 36.471 85 52 1 65 147 22 106 2.93e-17 75.5
MsaG008911 AT2G38880.1 36.471 85 52 1 65 147 22 106 2.93e-17 75.5
MsaG008911 AT2G38880.5 36.471 85 52 1 65 147 22 106 2.93e-17 75.5
MsaG008911 AT2G38880.3 36.471 85 52 1 65 147 22 106 4.70e-17 75.1
MsaG008911 AT2G38880.9 32.292 96 65 0 65 160 22 117 2.79e-16 73.2
MsaG008911 AT2G38880.10 32.292 96 65 0 65 160 22 117 2.79e-16 73.2
MsaG008911 AT2G38880.6 32.292 96 65 0 65 160 22 117 2.79e-16 73.2
MsaG008911 AT3G53340.5 32.558 129 81 2 65 187 30 158 2.95e-16 73.6
MsaG008911 AT3G53340.1 32.558 129 81 2 65 187 30 158 2.95e-16 73.6
MsaG008911 AT2G37060.2 33.673 98 64 1 47 144 14 110 3.01e-16 73.6
MsaG008911 AT2G37060.3 33.673 98 64 1 47 144 14 110 3.01e-16 73.6
MsaG008911 AT2G37060.1 33.673 98 64 1 47 144 14 110 3.01e-16 73.6
MsaG008911 AT3G53340.4 39.744 78 47 0 65 142 30 107 1.40e-15 70.5
MsaG008911 AT5G47670.2 37.037 81 51 0 64 144 29 109 2.32e-15 72.0
MsaG008911 AT4G14540.1 31.818 88 60 0 65 152 22 109 2.35e-15 70.9
MsaG008911 AT3G53340.3 34.375 96 63 0 64 159 29 124 2.70e-15 70.5
MsaG008911 AT3G53340.2 34.375 96 63 0 64 159 29 124 2.70e-15 70.5
MsaG008911 AT5G47670.1 37.037 81 51 0 64 144 58 138 3.63e-15 72.0
MsaG008911 AT5G47670.3 37.037 81 51 0 64 144 58 138 3.63e-15 72.0
MsaG008911 AT5G47640.1 33.735 83 55 0 60 142 23 105 2.06e-14 68.9
MsaG008911 AT1G21970.1 31.250 96 66 0 49 144 44 139 5.21e-14 68.9
MsaG008911 AT1G09030.1 33.750 80 53 0 65 144 4 83 2.03e-13 65.1
MsaG008911 AT2G13570.1 35.065 77 50 0 65 141 37 113 3.63e-13 66.2
MsaG008911 AT2G47810.1 31.250 80 55 0 65 144 52 131 2.23e-12 63.2

Find 46 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCATGTATTGAAAGCTTT+AGG 0.251459 2:-49509370 MsaT008911.1:CDS
TTATATCAGGTTCTTGGATT+TGG 0.252299 2:-49508567 MsaT008911.1:intron
TCATATTTATATCAGGTTCT+TGG 0.296989 2:-49508573 MsaT008911.1:intron
AAGAGCGAGGTGTGAATAGA+TGG 0.326834 2:-49510970 MsaT008911.1:CDS
CTGCAGCAAGACTCTATAAA+AGG 0.332402 2:-49507581 MsaT008911.1:intron
ACACACTCTTTCTCGTTGTT+TGG 0.349357 2:-49510883 MsaT008911.1:CDS
AACATTCAATTAACAAATCC+TGG 0.352577 2:+49510355 None:intergenic
GCACGTCATCAATTTGTTGC+GGG 0.374463 2:+49510946 None:intergenic
AGCATGTATTGAAAGCTTTA+GGG 0.375222 2:-49509369 MsaT008911.1:CDS
TCTAAACTTTGGTCAGCATC+TGG 0.377371 2:+49507443 None:intergenic
TTGGTCAGCATCTGGTTGAT+TGG 0.384189 2:+49507451 None:intergenic
TCTCTTGCAACACGCACATC+TGG 0.398229 2:+49510381 None:intergenic
AAAGTTAGTTCTCTAAACTT+TGG 0.415113 2:+49507432 None:intergenic
CTCTGCTAATGCTTGTTCCT+CGG 0.457821 2:+49507529 None:intergenic
TGATTTAGCAACAATATCCA+TGG 0.462776 2:+49510564 None:intergenic
AAAGCTTTCAATACATGCTC+AGG 0.488874 2:+49509373 None:intergenic
TGCACGTCATCAATTTGTTG+CGG 0.491047 2:+49510945 None:intergenic
GAGGCACGTGCTAGAATGAA+TGG 0.500006 2:-49507488 MsaT008911.1:CDS
CTCAGCTGCACCACTCCACT+TGG 0.501295 2:+49507556 None:intergenic
TACGTACTGGATGGATAAGA+AGG 0.517573 2:+49510858 None:intergenic
TATGTAACAGAGAGGAAAGA+AGG 0.521621 2:-49509405 MsaT008911.1:CDS
GAAGATGCTTCGCTACCTAA+AGG 0.522042 2:-49510538 MsaT008911.1:intron
TTGTTAATTGAATGTTGTGT+AGG 0.530693 2:-49510348 MsaT008911.1:intron
TCATAATAATAATACAAAGA+TGG 0.534014 2:-49510590 MsaT008911.1:CDS
GATTTAGCAACAATATCCAT+GGG 0.539741 2:+49510565 None:intergenic
TAATACAAAGATGGAGCCCA+TGG 0.543613 2:-49510581 MsaT008911.1:CDS
AAGCATTAGCAGAGCAGCAA+AGG 0.547129 2:-49507520 MsaT008911.1:CDS
CATTTGACTCTGATGAGACG+AGG 0.555057 2:+49509431 None:intergenic
AGTAAGCGCGTGCATGATGA+CGG 0.564255 2:-49510921 MsaT008911.1:CDS
TGGATTTGGCGAGTACATTG+AGG 0.592411 2:-49508553 MsaT008911.1:CDS
ACATTCAATTAACAAATCCT+GGG 0.595298 2:+49510356 None:intergenic
GGATATTGTTGCTAAATCAA+AGG 0.596085 2:-49510560 MsaT008911.1:CDS
AGCATATGAACAACACAAGA+TGG 0.603905 2:-49508520 MsaT008911.1:CDS
AGAGCGAGGTGTGAATAGAT+GGG 0.604063 2:-49510969 MsaT008911.1:CDS
GCATGTATTGAAAGCTTTAG+GGG 0.617694 2:-49509368 MsaT008911.1:intron
CTTGCAACACGCACATCTGG+AGG 0.618892 2:+49510384 None:intergenic
ATAAAAGGTGCCAAGTGGAG+TGG 0.637786 2:-49507566 MsaT008911.1:CDS
GCAGCAAAGGATGTTTGCAG+AGG 0.645184 2:-49507507 MsaT008911.1:CDS
GCGTGTTGCAAGAGATACCC+AGG 0.645605 2:-49510373 MsaT008911.1:CDS
ACTCTATAAAAGGTGCCAAG+TGG 0.653102 2:-49507571 MsaT008911.1:CDS
GCACGTGCTAGAATGAATGG+AGG 0.666011 2:-49507485 MsaT008911.1:CDS
CACGTGCTAGAATGAATGGA+GGG 0.682359 2:-49507484 MsaT008911.1:CDS
ACAACACAAGATGGAGACAG+TGG 0.698542 2:-49508511 MsaT008911.1:intron
AAATGAAGTATGTAACAGAG+AGG 0.704349 2:-49509413 MsaT008911.1:CDS
AAGCGCGTGCATGATGACGG+AGG 0.713947 2:-49510918 MsaT008911.1:CDS
TGGTGCAGCTGAGATGACCG+AGG 0.734859 2:-49507546 MsaT008911.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 49507436 49510999 49507436 ID=MsaG008911
Chr2 mRNA 49507436 49510999 49507436 ID=MsaT008911.1;Parent=MsaG008911
Chr2 exon 49507436 49507597 49507436 ID=MsaT008911.1.exon6;Parent=MsaT008911.1
Chr2 CDS 49507436 49507597 49507436 ID=cds.MsaT008911.1;Parent=MsaT008911.1
Chr2 exon 49508512 49508580 49508512 ID=MsaT008911.1.exon5;Parent=MsaT008911.1
Chr2 CDS 49508512 49508580 49508512 ID=cds.MsaT008911.1;Parent=MsaT008911.1
Chr2 exon 49509369 49509463 49509369 ID=MsaT008911.1.exon4;Parent=MsaT008911.1
Chr2 CDS 49509369 49509463 49509369 ID=cds.MsaT008911.1;Parent=MsaT008911.1
Chr2 exon 49510349 49510438 49510349 ID=MsaT008911.1.exon3;Parent=MsaT008911.1
Chr2 CDS 49510349 49510438 49510349 ID=cds.MsaT008911.1;Parent=MsaT008911.1
Chr2 exon 49510539 49510617 49510539 ID=MsaT008911.1.exon2;Parent=MsaT008911.1
Chr2 CDS 49510539 49510617 49510539 ID=cds.MsaT008911.1;Parent=MsaT008911.1
Chr2 exon 49510865 49510999 49510865 ID=MsaT008911.1.exon1;Parent=MsaT008911.1
Chr2 CDS 49510865 49510999 49510865 ID=cds.MsaT008911.1;Parent=MsaT008911.1
Gene Sequence

>MsaT008911.1

ATGAAGGAAGAGCGAGGTGTGAATAGATGGGCCCGCAACAAATTGATGACGTGCAGAGTAAGCGCGTGCATGATGACGGAGGCAGAAAGAAAGAACACACTCTTTCTCGTTGTTTGGTTCCTTCTTATCCATCCAATTAATCATAATAATAATACAAAGATGGAGCCCATGGATATTGTTGCTAAATCAAAGGAAGATGCTTCGCTACCTAAAGCGACCATGACGAAAATTATTAAAGAGATGTTGCCTCCAGATGTGCGTGTTGCAAGAGATACCCAGGATTTGTTAATTGAATGTTGTGTAGAGTTCATAAACCTCGTCTCATCAGAGTCAAATGAAGTATGTAACAGAGAGGAAAGAAGGACGATTGCACCTGAGCATGTATTGAAAGCTTTAGGGGTTCTTGGATTTGGCGAGTACATTGAGGAAGTTTATGCAGCATATGAACAACACAAGATGGAGACAGTGCAAGACTCTATAAAAGGTGCCAAGTGGAGTGGTGCAGCTGAGATGACCGAGGAACAAGCATTAGCAGAGCAGCAAAGGATGTTTGCAGAGGCACGTGCTAGAATGAATGGAGGGACTGTTACTTCCAATCAACCAGATGCTGACCAAAGTTTAGAGAACTAA
Protein sequence

>MsaT008911.1

MKEERGVNRWARNKLMTCRVSACMMTEAERKNTLFLVVWFLLIHPINHNNNTKMEPMDIVAKSKEDASLPKATMTKIIKEMLPPDVRVARDTQDLLIECCVEFINLVSSESNEVCNREERRTIAPEHVLKALGVLGFGEYIEEVYAAYEQHKMETVQDSIKGAKWSGAAEMTEEQALAEQQRMFAEARARMNGGTVTSNQPDADQSLEN*