AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG008968

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008968 MtrunA17_Chr2g0308011 84.163 221 26 1 1 221 1 212 1.80e-115 328
MsaG008968 MtrunA17_Chr4g0061761 49.677 155 47 3 36 161 39 191 6.06e-33 120
MsaG008968 MtrunA17_Chr2g0328021 47.059 136 60 3 37 172 62 185 7.19e-33 120
MsaG008968 MtrunA17_Chr4g0031411 75.385 65 15 1 36 99 74 138 3.61e-30 114
MsaG008968 MtrunA17_Chr8g0341671 78.333 60 13 0 37 96 20 79 6.02e-30 110
MsaG008968 MtrunA17_Chr8g0374251 65.789 76 19 1 37 105 68 143 9.08e-30 113
MsaG008968 MtrunA17_Chr8g0367161 63.158 76 28 0 32 107 27 102 1.12e-29 112
MsaG008968 MtrunA17_Chr7g0237921 56.522 92 25 1 38 114 77 168 1.13e-29 112
MsaG008968 MtrunA17_Chr4g0047001 48.000 125 61 2 38 160 56 178 1.79e-29 113
MsaG008968 MtrunA17_Chr4g0010151 49.107 112 44 2 36 147 81 179 2.35e-29 112
MsaG008968 MtrunA17_Chr3g0127201 80.702 57 11 0 36 92 26 82 3.73e-29 110
MsaG008968 MtrunA17_Chr7g0223311 75.000 64 16 0 36 99 76 139 3.73e-29 112
MsaG008968 MtrunA17_Chr8g0374111 64.000 75 27 0 37 111 29 103 4.43e-29 108
MsaG008968 MtrunA17_Chr3g0126201 64.384 73 26 0 26 98 40 112 7.71e-29 110
MsaG008968 MtrunA17_Chr2g0282441 75.862 58 14 0 31 88 19 76 2.38e-28 109
MsaG008968 MtrunA17_Chr4g0030051 65.169 89 26 2 36 119 40 128 3.80e-28 107
MsaG008968 MtrunA17_Chr2g0282051 68.657 67 20 1 27 93 36 101 1.11e-27 106
MsaG008968 MtrunA17_Chr2g0326001 63.158 76 20 1 16 91 27 94 1.17e-27 106
MsaG008968 MtrunA17_Chr2g0282511 62.857 70 26 0 15 84 23 92 1.40e-27 106
MsaG008968 MtrunA17_Chr5g0411951 82.353 51 9 0 37 87 43 93 1.65e-27 107
MsaG008968 MtrunA17_Chr3g0117601 80.000 55 11 0 33 87 57 111 1.91e-27 106
MsaG008968 MtrunA17_Chr8g0346551 78.182 55 12 0 37 91 24 78 2.10e-27 105
MsaG008968 MtrunA17_Chr1g0188851 70.690 58 17 0 36 93 42 99 5.12e-27 104
MsaG008968 MtrunA17_Chr8g0355261 68.421 57 18 0 36 92 117 173 4.16e-26 104
MsaG008968 MtrunA17_Chr7g0217581 64.286 70 24 1 37 106 232 300 6.93e-26 104
MsaG008968 MtrunA17_Chr4g0047761 77.193 57 12 1 37 93 72 127 2.27e-25 100
MsaG008968 MtrunA17_Chr1g0177251 69.492 59 18 0 33 91 8 66 2.75e-25 100
MsaG008968 MtrunA17_Chr2g0283811 51.765 85 38 1 26 107 36 120 3.10e-25 96.7
MsaG008968 MtrunA17_Chr6g0453321 55.844 77 30 1 20 92 115 191 4.22e-25 102
MsaG008968 MtrunA17_Chr3g0092111 50.000 94 42 1 17 105 111 204 6.42e-25 101
MsaG008968 MtrunA17_Chr7g0254161 68.519 54 17 0 37 90 101 154 1.19e-24 100
MsaG008968 MtrunA17_Chr4g0042481 64.407 59 21 0 37 95 122 180 3.25e-24 99.8
MsaG008968 MtrunA17_Chr6g0460721 65.455 55 19 0 37 91 89 143 1.28e-23 96.3
MsaG008968 MtrunA17_Chr6g0460731 66.667 54 18 0 37 90 96 149 6.16e-23 95.1
MsaG008968 MtrunA17_Chr5g0417551 63.492 63 23 0 37 99 127 189 8.43e-23 94.7
MsaG008968 MtrunA17_Chr5g0417601 66.071 56 19 0 35 90 138 193 1.50e-22 94.4
MsaG008968 MtrunA17_Chr5g0417561 63.492 63 23 0 37 99 119 181 1.79e-22 93.6
MsaG008968 MtrunA17_Chr5g0417571 64.912 57 20 0 36 92 137 193 1.90e-22 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG008968 AT5G60850.1 70.652 92 23 2 18 109 34 121 1.47e-39 138
MsaG008968 AT1G51700.1 82.540 63 11 0 37 99 33 95 8.59e-34 120
MsaG008968 AT3G45610.1 46.575 146 67 4 36 179 39 175 1.79e-32 118
MsaG008968 AT5G60200.1 64.935 77 23 1 34 110 52 124 3.67e-32 117
MsaG008968 AT3G55370.1 71.642 67 19 0 36 102 75 141 5.86e-31 116
MsaG008968 AT3G55370.2 71.642 67 19 0 36 102 75 141 6.69e-31 116
MsaG008968 AT3G55370.3 71.642 67 19 0 36 102 120 186 1.10e-30 116
MsaG008968 AT3G21270.1 92.308 52 4 0 37 88 29 80 1.45e-30 112
MsaG008968 AT5G66940.1 60.440 91 30 3 17 101 7 97 1.49e-30 112
MsaG008968 AT1G07640.1 82.759 58 9 1 37 93 21 78 2.53e-30 113
MsaG008968 AT1G07640.2 82.759 58 9 1 37 93 77 134 4.26e-30 114
MsaG008968 AT5G65590.1 74.242 66 17 0 37 102 41 106 4.41e-30 114
MsaG008968 AT1G07640.3 82.759 58 9 1 37 93 85 142 5.66e-30 114
MsaG008968 AT4G38000.1 59.756 82 26 1 17 91 14 95 1.71e-29 110
MsaG008968 AT2G28810.2 83.019 53 9 0 36 88 76 128 7.34e-29 110
MsaG008968 AT1G28310.1 80.702 57 11 0 37 93 27 83 9.80e-29 110
MsaG008968 AT2G28810.1 83.019 53 9 0 36 88 93 145 1.03e-28 110
MsaG008968 AT1G28310.2 80.702 57 11 0 37 93 51 107 1.32e-28 110
MsaG008968 AT2G37590.1 74.138 58 15 0 36 93 88 145 1.45e-28 110
MsaG008968 AT3G50410.1 79.661 59 11 1 37 94 30 88 2.37e-28 107
MsaG008968 AT3G61850.2 72.581 62 17 0 37 98 62 123 2.43e-28 108
MsaG008968 AT3G61850.3 72.581 62 17 0 37 98 62 123 2.43e-28 108
MsaG008968 AT3G61850.1 72.581 62 17 0 37 98 74 135 3.16e-28 108
MsaG008968 AT3G61850.5 72.581 62 17 0 37 98 62 123 4.05e-28 108
MsaG008968 AT3G61850.4 72.581 62 17 0 37 98 74 135 4.92e-28 108
MsaG008968 AT3G52440.1 58.228 79 33 0 28 106 16 94 5.74e-28 106
MsaG008968 AT3G52440.2 58.228 79 33 0 28 106 35 113 1.13e-27 106
MsaG008968 AT4G21050.1 66.667 72 23 1 39 109 26 97 1.49e-27 104
MsaG008968 AT2G28510.1 72.131 61 17 0 36 96 47 107 1.68e-27 106
MsaG008968 AT1G21340.1 66.176 68 23 0 29 96 30 97 3.08e-27 105
MsaG008968 AT5G02460.1 76.786 56 13 0 36 91 94 149 3.53e-27 107
MsaG008968 AT4G24060.1 75.000 56 14 0 36 91 52 107 2.35e-26 104
MsaG008968 AT4G00940.1 79.245 53 11 0 39 91 70 122 2.33e-25 100
MsaG008968 AT4G00940.3 79.245 53 11 0 39 91 70 122 2.33e-25 100
MsaG008968 AT4G00940.2 79.245 53 11 0 39 91 70 122 2.33e-25 100
MsaG008968 AT1G29160.1 58.571 70 29 0 37 106 62 131 2.66e-25 97.8
MsaG008968 AT2G46590.2 57.576 99 37 2 37 132 80 176 4.54e-25 101
MsaG008968 AT1G64620.1 79.592 49 10 0 36 84 48 96 4.55e-25 100
MsaG008968 AT2G46590.1 57.576 99 37 2 37 132 68 164 4.75e-25 101
MsaG008968 AT4G21040.1 61.429 70 27 0 39 108 27 96 6.85e-25 98.2
MsaG008968 AT2G34140.1 72.222 54 15 0 37 90 58 111 7.18e-25 96.7
MsaG008968 AT3G47500.1 58.621 87 20 3 37 119 110 184 7.78e-25 101
MsaG008968 AT5G39660.3 72.222 54 15 0 37 90 138 191 2.32e-24 100
MsaG008968 AT5G39660.1 72.222 54 15 0 37 90 138 191 2.32e-24 100
MsaG008968 AT5G39660.2 72.222 54 15 0 37 90 138 191 2.32e-24 100
MsaG008968 AT5G62940.1 80.000 50 10 0 38 87 74 123 2.42e-24 99.4
MsaG008968 AT1G47655.1 57.143 70 28 1 17 84 7 76 2.46e-24 96.3
MsaG008968 AT5G62430.1 46.154 104 55 1 37 139 54 157 5.83e-24 97.1
MsaG008968 AT1G69570.1 70.370 54 16 0 37 90 132 185 9.84e-24 98.2
MsaG008968 AT4G21080.1 60.294 68 27 0 39 106 27 94 3.18e-23 94.4
MsaG008968 AT1G26790.1 68.519 54 17 0 37 90 111 164 1.00e-22 95.1
MsaG008968 AT4G21030.1 58.333 60 25 0 39 98 23 82 1.18e-15 72.8

Find 52 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAATGATGTTGTTTCTTA+TGG 0.092194 2:+50788690 MsaT008968.1:CDS
CAGCAGTTATCAAATGAATT+TGG 0.217916 2:+50788771 MsaT008968.1:CDS
TCTTTGATTTCTTTGATTTA+CGG 0.270159 2:-50788494 None:intergenic
CCAATGATGTTGTTTCTTAT+GGG 0.276734 2:+50788691 MsaT008968.1:CDS
CTTTGATTTCTTTGATTTAC+GGG 0.312389 2:-50788493 None:intergenic
GATGTTGTTTCTTATGGGTT+TGG 0.359219 2:+50788696 MsaT008968.1:CDS
GCACCGTTGATGAGTCTTAC+TGG 0.380552 2:+50788820 MsaT008968.1:CDS
ATGTTGTTTCTTATGGGTTT+GGG 0.381004 2:+50788697 MsaT008968.1:CDS
ATGAATTTGGATCGTTGGAT+TGG 0.382150 2:+50788784 MsaT008968.1:CDS
GTAAGAACTGCCGTCGCTAC+TGG 0.382722 2:+50788427 MsaT008968.1:CDS
ACAGTGAAGGTTCAACCGTT+AGG 0.410245 2:+50788604 MsaT008968.1:CDS
TTACGGGAACAACCGCCAAC+AGG 0.419825 2:-50788477 None:intergenic
ATCAAATGAATTTGGATCGT+TGG 0.421631 2:+50788779 MsaT008968.1:CDS
TGAATTTGGATCGTTGGATT+GGG 0.437910 2:+50788785 MsaT008968.1:CDS
GATGATGATAATTCTGGTTA+TGG 0.445814 2:-50788308 None:intergenic
AATGCAAGAAATTCATTCCA+TGG 0.450556 2:+50788236 None:intergenic
CAGTGAAGGTTCAACCGTTA+GGG 0.455669 2:+50788605 MsaT008968.1:CDS
GCCGTCGCTACTGGACCAAA+GGG 0.461028 2:+50788436 MsaT008968.1:CDS
TGGCGTGCTTGCTGCTGAAA+CGG 0.467885 2:-50788538 None:intergenic
CCCATAAGAAACAACATCAT+TGG 0.473053 2:-50788691 None:intergenic
CACGCCAACACCACCTCCAC+TGG 0.474815 2:+50788554 MsaT008968.1:CDS
CTTACTGGACAAACTCTCAT+TGG 0.474868 2:+50788835 MsaT008968.1:CDS
TTATCAGCTTCCAGTGGAGG+TGG 0.483911 2:-50788564 None:intergenic
CTTCGTAACGTCCCTGTTGG+CGG 0.506836 2:+50788465 MsaT008968.1:CDS
TGCCGTCGCTACTGGACCAA+AGG 0.519849 2:+50788435 MsaT008968.1:CDS
ATGATAATTCTGGTTATGGT+GGG 0.521689 2:-50788304 None:intergenic
GCTTCCAGTGGAGGTGGTGT+TGG 0.521828 2:-50788558 None:intergenic
GATGATAATTCTGGTTATGG+TGG 0.523805 2:-50788305 None:intergenic
GTCCTTCGTAACGTCCCTGT+TGG 0.524276 2:+50788462 MsaT008968.1:CDS
GGATCGTTGGATTGGGAAGG+TGG 0.526946 2:+50788792 MsaT008968.1:CDS
TTTGGATCGTTGGATTGGGA+AGG 0.535121 2:+50788789 MsaT008968.1:CDS
GAAATTCATTCCATGGGAGG+TGG 0.546192 2:+50788243 MsaT008968.1:CDS
GCTTGCTGCTGAAACGGTGA+TGG 0.562137 2:-50788532 None:intergenic
CCCCTTTGGTCCAGTAGCGA+CGG 0.562593 2:-50788437 None:intergenic
ATTCATTTGATAACTGCTGA+TGG 0.567874 2:-50788767 None:intergenic
GACTTATCAGCTTCCAGTGG+AGG 0.568248 2:-50788567 None:intergenic
ATGCAAGAAATTCATTCCAT+GGG 0.569330 2:+50788237 MsaT008968.1:CDS
GATTCTCATTCTAACAGTGA+AGG 0.590117 2:+50788591 MsaT008968.1:CDS
TGTGGTACAGCACGGAGACT+CGG 0.595362 2:+50788734 MsaT008968.1:CDS
TATAACAGAGTTAACCCTAA+CGG 0.613913 2:-50788619 None:intergenic
CGTCGCTACTGGACCAAAGG+GGG 0.615140 2:+50788438 MsaT008968.1:CDS
CGCCAACAGGGACGTTACGA+AGG 0.615867 2:-50788464 None:intergenic
TAAATCAAAGAAATCAAAGA+CGG 0.630139 2:+50788497 MsaT008968.1:CDS
CAAGAAATTCATTCCATGGG+AGG 0.639402 2:+50788240 MsaT008968.1:CDS
TGGGAATGATCAAGTACATG+TGG 0.648978 2:+50788716 MsaT008968.1:CDS
TACGGGAACAACCGCCAACA+GGG 0.650932 2:-50788476 None:intergenic
TGGTTGAACTTGAAGTTACG+AGG 0.652946 2:-50788671 None:intergenic
CAAGTACATGTGGTACAGCA+CGG 0.660421 2:+50788726 MsaT008968.1:CDS
CCGTCGCTACTGGACCAAAG+GGG 0.676140 2:+50788437 MsaT008968.1:CDS
TCTGACTTATCAGCTTCCAG+TGG 0.676214 2:-50788570 None:intergenic
TGTCCAGTAAGACTCATCAA+CGG 0.728509 2:-50788823 None:intergenic
GTGTTGAAGGAATCACAGCG+AGG 0.735194 2:-50788354 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 50788237 50788902 50788237 ID=MsaG008968
Chr2 mRNA 50788237 50788902 50788237 ID=MsaT008968.1;Parent=MsaG008968
Chr2 exon 50788237 50788902 50788237 ID=MsaT008968.1.exon1;Parent=MsaT008968.1
Chr2 CDS 50788237 50788902 50788237 ID=cds.MsaT008968.1;Parent=MsaT008968.1
Gene Sequence

>MsaT008968.1

ATGCAAGAAATTCATTCCATGGGAGGTGGAAATCTTTTTTGCGTTGAGGAAGATAGAAGAATGAAAACCCACCATAACCAGAATTATCATCATCATCATCATCTTGCTTTGAAATGTCCTCGCTGTGATTCCTTCAACACAAAATTCTGCTACTACAACAACTACAACCTCTCTCAGCCTCGTCATTTTTGTAAGAACTGCCGTCGCTACTGGACCAAAGGGGGTGTCCTTCGTAACGTCCCTGTTGGCGGTTGTTCCCGTAAATCAAAGAAATCAAAGACGGCATCATCTTCTTCCATCACCGTTTCAGCAGCAAGCACGCCAACACCACCTCCACTGGAAGCTGATAAGTCAGATTCTCATTCTAACAGTGAAGGTTCAACCGTTAGGGTTAACTCTGTTATAGAAAATGAACAAGTTAATAGCTTCAGCAACCTCGTAACTTCAAGTTCAACCAATGATGTTGTTTCTTATGGGTTTGGGAATGATCAAGTACATGTGGTACAGCACGGAGACTCGGTGATGCTCAACCATCAGCAGTTATCAAATGAATTTGGATCGTTGGATTGGGAAGGTGGTGCTAGCACCGTTGATGAGTCTTACTGGACAAACTCTCATTGGTCTGATCATGTGAATCATTCATCCTCTCTTTTTCATCTTCCCTGA
Protein sequence

>MsaT008968.1

MQEIHSMGGGNLFCVEEDRRMKTHHNQNYHHHHHLALKCPRCDSFNTKFCYYNNYNLSQPRHFCKNCRRYWTKGGVLRNVPVGGCSRKSKKSKTASSSSITVSAASTPTPPPLEADKSDSHSNSEGSTVRVNSVIENEQVNSFSNLVTSSSTNDVVSYGFGNDQVHVVQHGDSVMLNHQQLSNEFGSLDWEGGASTVDESYWTNSHWSDHVNHSSSLFHLP*