AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG009018

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009018 MtrunA17_Chr2g0308521 95.571 700 27 2 21 716 20 719 0.0 1204
MsaG009018 MtrunA17_Chr4g0047931 34.132 167 75 5 19 185 6 137 2.25e-18 89.4
MsaG009018 MtrunA17_Chr2g0281431 38.346 133 59 4 21 153 3 112 4.79e-16 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009018 AT2G44430.1 35.262 726 312 28 21 716 49 646 7.01e-79 265
MsaG009018 AT3G60110.1 33.144 706 345 26 22 716 52 641 1.12e-69 240
MsaG009018 AT3G57980.1 30.242 744 361 29 22 716 16 650 7.34e-56 202
MsaG009018 AT2G42150.1 31.280 633 314 24 22 629 16 552 1.18e-55 201
MsaG009018 AT3G57980.2 30.201 745 362 29 22 716 16 652 2.13e-55 201

Find 233 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGCTTTGTTCTTCAATTT+TGG 0.021371 2:+51939940 None:intergenic
TGCTGCGCAGCCCGCAATTC+AGG 0.232135 2:+51940405 None:intergenic
TTATGCTTGGAGAGAGAATT+TGG 0.234903 2:+51940276 None:intergenic
AGAACCAGATCTGGCGGTTT+CGG 0.243253 2:-51945300 MsaT009018.1:CDS
GAACCAGATCTGGCGGTTTC+GGG 0.243863 2:-51945299 MsaT009018.1:CDS
GCTCAGCAACACGCTGTTTC+CGG 0.256673 2:+51945548 None:intergenic
GGTGAGGTTGATGTAAAATT+AGG 0.274776 2:-51945083 MsaT009018.1:CDS
TTCCTTCTTGTCATTGATGA+TGG 0.282552 2:+51940167 None:intergenic
GTCAAGGTCGTTGCAGTTGC+AGG 0.303731 2:-51945375 MsaT009018.1:intron
TCGTCGTTCACCTGAATTGC+GGG 0.313585 2:-51940415 MsaT009018.1:CDS
CTGAGGAGTGGTAGTGGTGG+TGG 0.331369 2:-51939838 MsaT009018.1:CDS
TCCGATTCTTCACCCGTTGC+TGG 0.331509 2:+51945170 None:intergenic
CTCTTGGACCCTCAGTTGTA+TGG 0.343899 2:+51940515 None:intergenic
AGAGGGTGGTTTCTTATTGC+TGG 0.347250 2:+51940011 None:intergenic
AGTGGTAGTGGTGGTGGAGG+AGG 0.353098 2:-51939832 MsaT009018.1:CDS
AGAGAATTTGGTCTGGATAA+TGG 0.359430 2:+51940288 None:intergenic
TTCGGGCGACGAACGGTTGC+CGG 0.364328 2:-51945282 MsaT009018.1:CDS
TGGCTAAAGGTGGTGTTAGA+AGG 0.364479 2:+51940207 None:intergenic
CGAGGAGGACGCAGCGGCGG+AGG 0.373416 2:-51944800 MsaT009018.1:CDS
TCTTCATCTGTCTCCGAACT+TGG 0.375453 2:+51940111 None:intergenic
TCCTTCTTGTCATTGATGAT+GGG 0.378342 2:+51940168 None:intergenic
TCTAACTCCACTCCTAAAAC+AGG 0.378746 2:-51939991 MsaT009018.1:CDS
GGTCGGAGGAGGGAGGGACG+AGG 0.389910 2:-51944863 MsaT009018.1:CDS
AGGAGTGGTAGTGGTGGTGG+AGG 0.392175 2:-51939835 MsaT009018.1:CDS
ACCGCCATGGCTTCAAAGAC+TGG 0.403316 2:-51945762 MsaT009018.1:CDS
AGGAAGTAGTGGAAGCAGTA+AGG 0.404761 2:-51939812 MsaT009018.1:CDS
TGGGTTTGTGATCACCCTTT+TGG 0.405811 2:+51940187 None:intergenic
GATGTAGGAGGGTCAGGTTC+GGG 0.407651 2:-51939694 MsaT009018.1:CDS
CCGAAGAAAATAGTGAATAA+TGG 0.411418 2:-51939757 MsaT009018.1:CDS
GGTCTGGATAATGGTGTGTT+TGG 0.413553 2:+51940297 None:intergenic
AAAATAGTGAATAATGGAAA+AGG 0.414108 2:-51939751 MsaT009018.1:CDS
TTGAATGAACCGGATCAAAC+CGG 0.416831 2:-51945014 MsaT009018.1:CDS
TCAGGTCCAGGTCCTAGTTC+CGG 0.417956 2:-51945059 MsaT009018.1:CDS
TCCACCTTCCTTTCCTCACT+CGG 0.419312 2:+51944903 None:intergenic
GGAAGCAGTAAGGGTGGTGG+TGG 0.419982 2:-51939802 MsaT009018.1:CDS
AAATAGTGAATAATGGAAAA+GGG 0.420047 2:-51939750 MsaT009018.1:CDS
GAGGTGCTGAGGAGTGGTAG+TGG 0.422126 2:-51939844 MsaT009018.1:CDS
AGCAGTAAGGGTGGTGGTGG+AGG 0.424109 2:-51939799 MsaT009018.1:CDS
ATGAACCGGTACCGGATATT+CGG 0.424207 2:-51945232 MsaT009018.1:CDS
CTGGATAATGGTGTGTTTGG+AGG 0.430275 2:+51940300 None:intergenic
CGATGTAGGAGGGTCAGGTT+CGG 0.433879 2:-51939695 MsaT009018.1:CDS
CGTTTGCGGCAAACTAATAT+AGG 0.436236 2:+51940249 None:intergenic
AAAATCCGGTTTGAATGAAC+CGG 0.437047 2:-51945024 MsaT009018.1:CDS
TCGCCCGAAACCGCCAGATC+TGG 0.437905 2:+51945296 None:intergenic
ACCTACAGGGTTGGGGCACG+TGG 0.438220 2:-51945813 MsaT009018.1:intron
CACCGTTCTGCTGTGGCGGC+GGG 0.443560 2:+51945626 None:intergenic
TGTGAAACGGCACGTGGACT+TGG 0.443952 2:-51940541 MsaT009018.1:CDS
TGGATAATGGTGTGTTTGGA+GGG 0.444053 2:+51940301 None:intergenic
AGGTTTGATGCATTGAAAAC+CGG 0.445177 2:-51945104 MsaT009018.1:CDS
CGGTGGAGATATGGATGAAC+CGG 0.450462 2:-51945246 MsaT009018.1:CDS
CAATGCATCAAACCTCGAAC+CGG 0.450475 2:+51945112 None:intergenic
TCATCGAGCCGCCGAATATC+CGG 0.453714 2:+51945221 None:intergenic
GTTGATGTAAAATTAGGTTC+AGG 0.454358 2:-51945077 MsaT009018.1:CDS
CAAGAGGGTCACTATTCCTC+AGG 0.458008 2:-51940498 MsaT009018.1:CDS
AGTAAGGGTGGTGGTGGAGG+TGG 0.458460 2:-51939796 MsaT009018.1:CDS
AGAAGAAACAGAACCAGATC+TGG 0.458716 2:-51945309 MsaT009018.1:CDS
CTGGGATTGAACTACGTTTG+CGG 0.459098 2:+51940235 None:intergenic
ACCAGCAACGGGTGAAGAAT+CGG 0.462254 2:-51945171 MsaT009018.1:CDS
TAACACCACCTTTAGCCAAA+AGG 0.463730 2:-51940202 MsaT009018.1:CDS
GGAAGTAGTGGAAGCAGTAA+GGG 0.463770 2:-51939811 MsaT009018.1:CDS
GAGGACGCAGCGGCGGAGGA+AGG 0.465986 2:-51944796 MsaT009018.1:CDS
TCCAGGTCCTAGTTCCGGTT+CGG 0.468471 2:-51945054 MsaT009018.1:CDS
GTGGTGTTAGAAGGTTTGAC+TGG 0.468509 2:+51940216 None:intergenic
CTACTTCTAGCTCCAGTGAC+AGG 0.468988 2:+51940063 None:intergenic
AATAATGGAAAAGGGGAGAA+AGG 0.470625 2:-51939742 MsaT009018.1:CDS
ACCAACCTTAAAACCAAGTT+CGG 0.470995 2:-51940124 MsaT009018.1:CDS
ACTTGAACCGAACCGGAACT+AGG 0.471373 2:+51945047 None:intergenic
GCCAAGGAAAAGCCTGTCAC+TGG 0.474414 2:-51940075 MsaT009018.1:CDS
TTGATCCGGTTCATTCAAAC+CGG 0.475102 2:+51945019 None:intergenic
AACACCACCTTTAGCCAAAA+GGG 0.477578 2:-51940201 MsaT009018.1:CDS
TCACCGTTCTGCTGTGGCGG+CGG 0.481581 2:+51945625 None:intergenic
GCGGGACGTCGTCTTTAAAT+CGG 0.483234 2:+51945644 None:intergenic
AACCGGGAGGAAAAGAAAAT+CGG 0.483363 2:-51944997 MsaT009018.1:CDS
GACGCAGCGGCGGAGGAAGG+AGG 0.487237 2:-51944793 MsaT009018.1:CDS
TATAACGATGTAGGAGGGTC+AGG 0.488606 2:-51939700 MsaT009018.1:CDS
GGTGGTGGAGGAGGAAGTAG+TGG 0.488897 2:-51939823 MsaT009018.1:CDS
AGGGTCCTCGGCAAACAAAC+CGG 0.489839 2:-51939971 MsaT009018.1:CDS
GTTAATGAGTCAAACTCAAC+CGG 0.491137 2:-51945131 MsaT009018.1:CDS
CGGCAGCGTGGTGGTGATGA+CGG 0.491465 2:-51946673 MsaT009018.1:CDS
GATAAGTTGTTACCAGCAAC+GGG 0.491689 2:-51945182 MsaT009018.1:CDS
TTAGGAGTGGAGTTAGAGGG+TGG 0.496127 2:+51939997 None:intergenic
TGGCGGTTTCGGGCGACGAA+CGG 0.496700 2:-51945289 MsaT009018.1:CDS
GTCGGTGACTCGGTCGGAGG+AGG 0.499817 2:-51944874 MsaT009018.1:CDS
CACTCCTAAAACAGGGTCCT+CGG 0.507185 2:-51939983 MsaT009018.1:CDS
AGCAACTCGAAGAGGAATGT+TGG 0.507500 2:-51939646 MsaT009018.1:CDS
CTTGGAGAGAGAATTTGGTC+TGG 0.508801 2:+51940281 None:intergenic
CGAGAAAGCCAAAGAAAATG+AGG 0.509814 2:-51945336 MsaT009018.1:CDS
AAGGGAAAGAAAGAGCTTCA+AGG 0.510393 2:-51939723 MsaT009018.1:CDS
GTGCTGAGGAGTGGTAGTGG+TGG 0.511659 2:-51939841 MsaT009018.1:CDS
GGTCAGGTTCGGGAGATAAG+AGG 0.513401 2:-51939684 MsaT009018.1:CDS
AAGAAGAACAACGGTGCCGC+TGG 0.514978 2:-51939901 MsaT009018.1:CDS
AAGATTCCGATCACGTTCCA+TGG 0.516356 2:-51945582 MsaT009018.1:CDS
AAGGAAGGTGGAGGATGAGT+CGG 0.517097 2:-51944892 MsaT009018.1:CDS
CTCAGCCGGTTTGTTTGCCG+AGG 0.518548 2:+51939966 None:intergenic
AGAACCGTTGGTTTGCGTGT+TGG 0.518675 2:-51944727 MsaT009018.1:CDS
GGTTCGGTTCAAGTAAAATC+CGG 0.519685 2:-51945038 MsaT009018.1:CDS
GAATGGAGACGTCGCTGCGT+TGG 0.520177 2:+51945512 None:intergenic
TGATAGTGTTGGATTGTGCT+TGG 0.520415 2:+51940343 None:intergenic
GTGGTGTGCGGTGGCGAGGA+GGG 0.521084 2:+51945696 None:intergenic
GGCGAGGAGGGAAGTGCGGT+TGG 0.521102 2:+51945708 None:intergenic
GTTGGTTTCACATCAGAGAA+AGG 0.523554 2:+51940141 None:intergenic
AGTAGTGGAAGCAGTAAGGG+TGG 0.528511 2:-51939808 MsaT009018.1:CDS
CACAGTGGTGTGCGGTGGCG+AGG 0.530616 2:+51945692 None:intergenic
GCCATGGCGGTCGACGGCGG+AGG 0.530665 2:+51945774 None:intergenic
TCCACGCTTTGTACTAGCTA+CGG 0.531807 2:+51939597 None:intergenic
GGAGAATGAGGACGTGGCGG+TGG 0.532383 2:-51944757 MsaT009018.1:CDS
CTGAGGAATAGTGACCCTCT+TGG 0.532787 2:+51940499 None:intergenic
TGATAAGTTGTTACCAGCAA+CGG 0.533007 2:-51945183 MsaT009018.1:CDS
CTTGATGAGATGAAAACCTT+CGG 0.533167 2:-51940366 MsaT009018.1:CDS
GTGACTCGGTCGGAGGAGGG+AGG 0.534084 2:-51944870 MsaT009018.1:CDS
TCGGTGACTCGGTCGGAGGA+GGG 0.536765 2:-51944873 MsaT009018.1:CDS
AAATTGAAGAACAAAGCAGA+GGG 0.540990 2:-51939937 MsaT009018.1:CDS
ATCGGAATCTTCAGAGGTGG+CGG 0.541241 2:+51945594 None:intergenic
TAGCTAGTACAAAGCGTGGA+AGG 0.541332 2:-51939594 MsaT009018.1:CDS
AGATATGGATGAACCGGTAC+CGG 0.543994 2:-51945240 MsaT009018.1:CDS
GCTCTTCTGTGTGATAGTGT+TGG 0.545020 2:+51940332 None:intergenic
TATTGCTGGCGTTGCTGCTG+AGG 0.545133 2:+51940025 None:intergenic
GCTTCAAGGTATAACGATGT+AGG 0.546050 2:-51939709 MsaT009018.1:CDS
AACCAAGACCTACAGGGTTG+GGG 0.547415 2:-51945820 MsaT009018.1:intron
TTTGAAGCCATGGCGGTCGA+CGG 0.547704 2:+51945768 None:intergenic
AGTCAAACTCAACCGGTTCG+AGG 0.548246 2:-51945124 MsaT009018.1:CDS
ATAATGGAAAAGGGGAGAAA+GGG 0.549271 2:-51939741 MsaT009018.1:CDS
TAATATAGGAGGTTTATGCT+TGG 0.549559 2:+51940263 None:intergenic
AGACGAGGAGGACGCAGCGG+CGG 0.550143 2:-51944803 MsaT009018.1:CDS
AATAGTGAATAATGGAAAAG+GGG 0.551013 2:-51939749 MsaT009018.1:CDS
TTCCGGAGTTTATCAAGCCA+TGG 0.551475 2:+51945565 None:intergenic
CTGATGGTGGTGATGACGGC+GGG 0.552252 2:-51946696 MsaT009018.1:CDS
CGGTTCATCCATATCTCCAC+CGG 0.553015 2:+51945247 None:intergenic
CTCGTCGTTCACCTGAATTG+CGG 0.556541 2:-51940416 MsaT009018.1:CDS
ACCCATCATCAATGACAAGA+AGG 0.556669 2:-51940169 MsaT009018.1:CDS
AGTGGAAGCAGTAAGGGTGG+TGG 0.557192 2:-51939805 MsaT009018.1:CDS
ATCAAGCCATGGAACGTGAT+CGG 0.558315 2:+51945576 None:intergenic
AGACACAAAGACGGAGAATG+AGG 0.558418 2:-51944769 MsaT009018.1:CDS
GAAGCCATGGCGGTCGACGG+CGG 0.558784 2:+51945771 None:intergenic
TGACTCGGTCGGAGGAGGGA+GGG 0.560328 2:-51944869 MsaT009018.1:CDS
AGTGGTGTGCGGTGGCGAGG+AGG 0.563406 2:+51945695 None:intergenic
AAGTTCGGAGACAGATGAAG+AGG 0.565785 2:-51940109 MsaT009018.1:CDS
AGATGAAGAGGAGCCTCCTA+AGG 0.566505 2:-51940097 MsaT009018.1:CDS
GTAAAATTAGGTTCAGGTCC+AGG 0.567794 2:-51945071 MsaT009018.1:CDS
CAACGGGTGAAGAATCGGAC+CGG 0.569525 2:-51945166 MsaT009018.1:CDS
TGAAGAGAGTAACGAGGTCA+AGG 0.572106 2:+51940460 None:intergenic
AGCCGCCGAATATCCGGTAC+CGG 0.573032 2:+51945227 None:intergenic
AGCTAGTACAAAGCGTGGAA+GGG 0.576027 2:-51939593 MsaT009018.1:CDS
CCGCCATGGCTTCAAAGACT+GGG 0.576184 2:-51945761 MsaT009018.1:CDS
TGGTGTTAGAAGGTTTGACT+GGG 0.576970 2:+51940217 None:intergenic
ACCGAACCGGAACTAGGACC+TGG 0.577356 2:+51945053 None:intergenic
GAAGGAGGAAGACACAAAGA+CGG 0.577695 2:-51944778 MsaT009018.1:CDS
TGTCCCAGTCTTTGAAGCCA+TGG 0.577926 2:+51945758 None:intergenic
GAGTGAGGAAAGGAAGGTGG+AGG 0.578912 2:-51944901 MsaT009018.1:CDS
TGAATGAACCGGATCAAACC+GGG 0.579010 2:-51945013 MsaT009018.1:CDS
TCAAGGTATAACGATGTAGG+AGG 0.580484 2:-51939706 MsaT009018.1:CDS
TTGCGGCAAACTAATATAGG+AGG 0.580492 2:+51940252 None:intergenic
GCCTCCGCCGTCGACCGCCA+TGG 0.581687 2:-51945775 MsaT009018.1:CDS
GGAATCTTCAGAGGTGGCGG+CGG 0.582736 2:+51945597 None:intergenic
GACGGCGGGCGGCAGCGTGG+TGG 0.584138 2:-51946682 MsaT009018.1:CDS
TTCCATGGCTTGATAAACTC+CGG 0.584964 2:-51945567 MsaT009018.1:CDS
GGTGGTGAAATCAGAACCGT+TGG 0.585788 2:-51944739 MsaT009018.1:CDS
ACGTCACCGTTCTGCTGTGG+CGG 0.586498 2:+51945622 None:intergenic
GTTGGATTGTGCTTGGCCGA+AGG 0.589323 2:+51940350 None:intergenic
CGGTGCCTCCGAGTGAGGAA+AGG 0.592815 2:-51944911 MsaT009018.1:CDS
TGGAGGATGAGTCGGTGACT+CGG 0.593698 2:-51944884 MsaT009018.1:CDS
CATTAACAGATTGATTATCC+CGG 0.593721 2:+51945147 None:intergenic
CGATCTGATGGTGGTGATGA+CGG 0.596661 2:-51946700 None:intergenic
GGTACGTGACTGAACCTCCA+TGG 0.597587 2:+51945729 None:intergenic
TCATCAATGACAAGAAGGAA+AGG 0.597938 2:-51940164 MsaT009018.1:CDS
GAGGAAAAGAAAATCGGTTG+AGG 0.601160 2:-51944991 MsaT009018.1:CDS
GATGACGGCGGGCGGCAGCG+TGG 0.601697 2:-51946685 MsaT009018.1:CDS
ACCGTAGCTAGTACAAAGCG+TGG 0.602506 2:-51939598 MsaT009018.1:CDS
GTCGGAGGAGGGAGGGACGA+GGG 0.604049 2:-51944862 MsaT009018.1:CDS
ATCTCCAACACGCAAACCAA+CGG 0.605003 2:+51944723 None:intergenic
GCCTCCGAGTGAGGAAAGGA+AGG 0.605677 2:-51944907 MsaT009018.1:CDS
TGAGTCGGTGCCTCCGAGTG+AGG 0.608632 2:-51944916 MsaT009018.1:CDS
GTTGAGGAAGAATCGAATAA+CGG 0.610392 2:-51944975 MsaT009018.1:CDS
AGACTGGGACACCATCGCCA+TGG 0.614420 2:-51945746 MsaT009018.1:CDS
CGTCGAGTTTGTCGAAGACG+AGG 0.614539 2:-51944818 MsaT009018.1:CDS
ATTGAAGAACAAAGCAGAGG+GGG 0.614576 2:-51939935 MsaT009018.1:CDS
GGATGAGTCGGTGACTCGGT+CGG 0.614727 2:-51944880 MsaT009018.1:CDS
CTTGGAAACCATACAACTGA+GGG 0.617776 2:-51940523 MsaT009018.1:CDS
CGGTGGCGAGGAGGGAAGTG+CGG 0.618822 2:+51945704 None:intergenic
AGAGGAGCCTCCTAAGGCCA+AGG 0.619461 2:-51940091 MsaT009018.1:CDS
ATGAATCGGTGGAGGTGCTG+AGG 0.620396 2:-51939855 MsaT009018.1:CDS
TTGTTCACAGTGGTGTGCGG+TGG 0.621124 2:+51945687 None:intergenic
GAGACGTCACCGTTCTGCTG+TGG 0.626958 2:+51945619 None:intergenic
TAAAGCTGTTACGTGTGAGT+CGG 0.627421 2:-51944931 MsaT009018.1:CDS
TCCGAGTGAGGAAAGGAAGG+TGG 0.627569 2:-51944904 MsaT009018.1:CDS
TCATCTCATCAAGGATGAGG+CGG 0.630133 2:+51940376 None:intergenic
AACGGGTGAAGAATCGGACC+GGG 0.631167 2:-51945165 MsaT009018.1:CDS
GGACGTCGTCTTTAAATCGG+CGG 0.635775 2:+51945647 None:intergenic
ACCTTCCTTTCCTCACTCGG+AGG 0.638244 2:+51944906 None:intergenic
GTCCCGCCGCCACAGCAGAA+CGG 0.640917 2:-51945628 MsaT009018.1:CDS
GACGGCGGACGGCTGCGTGG+TGG 0.641864 2:-51946655 MsaT009018.1:intron
TGACTGAACCTCCATGGCGA+TGG 0.644432 2:+51945735 None:intergenic
GGAAGGGAAAGTAGTGCAAG+TGG 0.645130 2:-51939577 MsaT009018.1:CDS
GATGACGGCGGACGGCTGCG+TGG 0.645196 2:-51946658 MsaT009018.1:CDS
ATATTGAGAGCAACTCGAAG+AGG 0.645471 2:-51939654 MsaT009018.1:CDS
CCCAGTCTTTGAAGCCATGG+CGG 0.646632 2:+51945761 None:intergenic
AGGGACGAGGGAGAGCAGCG+AGG 0.649215 2:-51944850 MsaT009018.1:CDS
AAAATTGAAGAACAAAGCAG+AGG 0.652415 2:-51939938 MsaT009018.1:CDS
AAATACTGTGAAACGGCACG+TGG 0.652877 2:-51940547 MsaT009018.1:CDS
ATGGTGGTGATGACGGCGGG+CGG 0.652897 2:-51946693 MsaT009018.1:CDS
AAAGACGGAGAATGAGGACG+TGG 0.656597 2:-51944763 MsaT009018.1:CDS
AAGAGGGTCACTATTCCTCA+GGG 0.657747 2:-51940497 MsaT009018.1:CDS
TCGGTGGAGGTGCTGAGGAG+TGG 0.663383 2:-51939850 MsaT009018.1:CDS
AACGTGATCGGAATCTTCAG+AGG 0.663851 2:+51945588 None:intergenic
AGAAACAGAACCAGATCTGG+CGG 0.666736 2:-51945306 MsaT009018.1:CDS
AACCGGTACCGGATATTCGG+CGG 0.667366 2:-51945229 MsaT009018.1:CDS
GTGATCGGAATCTTCAGAGG+TGG 0.669707 2:+51945591 None:intergenic
CAAGGTATAACGATGTAGGA+GGG 0.672081 2:-51939705 MsaT009018.1:CDS
CGAAGACGAGGAGGACGCAG+CGG 0.673366 2:-51944806 MsaT009018.1:CDS
GTGGTGGTGATGACGGCGGA+CGG 0.673969 2:-51946666 MsaT009018.1:CDS
CTAACTCCACTCCTAAAACA+GGG 0.675031 2:-51939990 MsaT009018.1:CDS
GAAGAATGTGTTTGTCCCTG+AGG 0.676500 2:+51940482 None:intergenic
CCTACAGGGTTGGGGCACGT+GGG 0.678232 2:-51945812 MsaT009018.1:intron
GACGGAGAATGAGGACGTGG+CGG 0.681037 2:-51944760 MsaT009018.1:CDS
TGAGTCGGTGACTCGGTCGG+AGG 0.681570 2:-51944877 MsaT009018.1:CDS
TATTCTATTCAAGAAACCAG+CGG 0.683143 2:+51939885 None:intergenic
CATACAACTGAGGGTCCAAG+AGG 0.686334 2:-51940514 MsaT009018.1:CDS
CGAGTTTGTCGAAGACGAGG+AGG 0.686373 2:-51944815 MsaT009018.1:CDS
AGGGAAAGTAGTGCAAGTGG+TGG 0.690401 2:-51939574 MsaT009018.1:CDS
CTGGGACACCATCGCCATGG+AGG 0.691536 2:-51945743 MsaT009018.1:CDS
TCTGATGGTGGTGATGACGG+CGG 0.695012 2:-51946697 None:intergenic
CAGCGTGGTGGTGATGACGG+CGG 0.705331 2:-51946670 MsaT009018.1:CDS
ACTTGGAAACCATACAACTG+AGG 0.710642 2:-51940524 MsaT009018.1:CDS
CGCTGCGTTGGACATCACGA+CGG 0.716962 2:+51945524 None:intergenic
ATACAACTGAGGGTCCAAGA+GGG 0.731011 2:-51940513 MsaT009018.1:CDS
TCAGACAAGAAGAAGAACAA+CGG 0.731120 2:-51939910 MsaT009018.1:CDS
CATTGCTGAAGAGAGTAACG+AGG 0.733636 2:+51940454 None:intergenic
AATTGAAGAACAAAGCAGAG+GGG 0.751790 2:-51939936 MsaT009018.1:CDS
TGATGCATTGAAAACCGGTG+AGG 0.759977 2:-51945099 MsaT009018.1:CDS
TGAACGACGAGAGAAGAACA+CGG 0.764187 2:+51940428 None:intergenic
ATGAACCGGATCAAACCGGG+AGG 0.766425 2:-51945010 MsaT009018.1:CDS
CGGAAGCTATGATAACAACG+AGG 0.775503 2:-51944955 MsaT009018.1:CDS
ACGACGAGAGAAGAACACGG+TGG 0.843016 2:+51940431 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 51939537 51946715 51939537 ID=MsaG009018
Chr2 mRNA 51939537 51946715 51939537 ID=MsaT009018.1;Parent=MsaG009018
Chr2 exon 51939537 51940586 51939537 ID=MsaT009018.1.exon4;Parent=MsaT009018.1
Chr2 CDS 51939537 51940586 51939537 ID=cds.MsaT009018.1;Parent=MsaT009018.1
Chr2 exon 51944668 51945391 51944668 ID=MsaT009018.1.exon3;Parent=MsaT009018.1
Chr2 CDS 51944668 51945391 51944668 ID=cds.MsaT009018.1;Parent=MsaT009018.1
Chr2 exon 51945511 51945827 51945511 ID=MsaT009018.1.exon2;Parent=MsaT009018.1
Chr2 CDS 51945511 51945827 51945511 ID=cds.MsaT009018.1;Parent=MsaT009018.1
Chr2 exon 51946656 51946715 51946656 ID=MsaT009018.1.exon1;Parent=MsaT009018.1
Chr2 CDS 51946656 51946715 51946656 ID=cds.MsaT009018.1;Parent=MsaT009018.1
Gene Sequence

>MsaT009018.1

ATGGTGGTGATGACGGCGGGCGGCAGCGTGGTGGTGATGACGGCGGACGGCTGCGTGGTGGGTTGGGGCACGTGGGAAGAGCTTTTACTAGCCTCCGCCGTCGACCGCCATGGCTTCAAAGACTGGGACACCATCGCCATGGAGGTTCAGTCACGTACCAACCGCACTTCCCTCCTCGCCACCGCACACCACTGTGAACAAAAATTTCACGATCTAAACCGCCGATTTAAAGACGACGTCCCGCCGCCACAGCAGAACGGTGACGTCTCCGCCGCCACCTCTGAAGATTCCGATCACGTTCCATGGCTTGATAAACTCCGGAAACAGCGTGTTGCTGAGCTCCGTCGTGATGTCCAACGCAGCGACGTCTCCATTCTGTCGTTGCAGTTGCAGGTGAAAAAATTAGAAGACGAGAAAGCCAAAGAAAATGAGGAAAAAGAAGAAACAGAACCAGATCTGGCGGTTTCGGGCGACGAACGGTTGCCGGAGAATGAAAAAACCGGTGGAGATATGGATGAACCGGTACCGGATATTCGGCGGCTCGATGAAAGCACAACTAATACTGATAAGTTGTTACCAGCAACGGGTGAAGAATCGGACCGGGATAATCAATCTGTTAATGAGTCAAACTCAACCGGTTCGAGGTTTGATGCATTGAAAACCGGTGAGGTTGATGTAAAATTAGGTTCAGGTCCAGGTCCTAGTTCCGGTTCGGTTCAAGTAAAATCCGGTTTGAATGAACCGGATCAAACCGGGAGGAAAAGAAAATCGGTTGAGGAAGAATCGAATAACGGAAGCTATGATAACAACGAGGCTAAAGCTGTTACGTGTGAGTCGGTGCCTCCGAGTGAGGAAAGGAAGGTGGAGGATGAGTCGGTGACTCGGTCGGAGGAGGGAGGGACGAGGGAGAGCAGCGAGGTGCAGAGTTCGTCGAGTTTGTCGAAGACGAGGAGGACGCAGCGGCGGAGGAAGGAGGAAGACACAAAGACGGAGAATGAGGACGTGGCGGTGGTGAAATCAGAACCGTTGGTTTGCGTGTTGGAGATGATCAAAAGACATGAAAAATTTTCATTGTTTGAGCGCCGTCTTGAAAAAAACCAGGATTTGGACAGATACAAAAATACTGTGAAACGGCACGTGGACTTGGAAACCATACAACTGAGGGTCCAAGAGGGTCACTATTCCTCAGGGACAAACACATTCTTCCTTGACCTCGTTACTCTCTTCAGCAATGCCACCGTGTTCTTCTCTCGTCGTTCACCTGAATTGCGGGCTGCGCAGCAGCTGCGCCGCCTCATCCTTGATGAGATGAAAACCTTCGGCCAAGCACAATCCAACACTATCACACAGAAGAGCGATTCACTCCCTCCAAACACACCATTATCCAGACCAAATTCTCTCTCCAAGCATAAACCTCCTATATTAGTTTGCCGCAAACGTAGTTCAATCCCAGTCAAACCTTCTAACACCACCTTTAGCCAAAAGGGTGATCACAAACCCATCATCAATGACAAGAAGGAAAGGCCTTTCTCTGATGTGAAACCAACCTTAAAACCAAGTTCGGAGACAGATGAAGAGGAGCCTCCTAAGGCCAAGGAAAAGCCTGTCACTGGAGCTAGAAGTAGAAGAAGTAACAAGAACCTCAGCAGCAACGCCAGCAATAAGAAACCACCCTCTAACTCCACTCCTAAAACAGGGTCCTCGGCAAACAAACCGGCTGAGACCCCAAAATTGAAGAACAAAGCAGAGGGGGTGTCAGACAAGAAGAAGAACAACGGTGCCGCTGGTTTCTTGAATAGAATAAAGAAAAATGAATCGGTGGAGGTGCTGAGGAGTGGTAGTGGTGGTGGAGGAGGAAGTAGTGGAAGCAGTAAGGGTGGTGGTGGAGGTGGTACTAATACAAAAGAGCCGAAGAAAATAGTGAATAATGGAAAAGGGGAGAAAGGGAAAGAAAGAGCTTCAAGGTATAACGATGTAGGAGGGTCAGGTTCGGGAGATAAGAGGAACAAGAATATTGAGAGCAACTCGAAGAGGAATGTTGGAAGACCTCCAAAAAAAGCGGCAGAGACCGTAGCTAGTACAAAGCGTGGAAGGGAAAGTAGTGCAAGTGGTGGCAAAGATAAGCGACCCAAAAAACGTTCTAAGAAATGA
Protein sequence

>MsaT009018.1

MVVMTAGGSVVVMTADGCVVGWGTWEELLLASAVDRHGFKDWDTIAMEVQSRTNRTSLLATAHHCEQKFHDLNRRFKDDVPPPQQNGDVSAATSEDSDHVPWLDKLRKQRVAELRRDVQRSDVSILSLQLQVKKLEDEKAKENEEKEETEPDLAVSGDERLPENEKTGGDMDEPVPDIRRLDESTTNTDKLLPATGEESDRDNQSVNESNSTGSRFDALKTGEVDVKLGSGPGPSSGSVQVKSGLNEPDQTGRKRKSVEEESNNGSYDNNEAKAVTCESVPPSEERKVEDESVTRSEEGGTRESSEVQSSSSLSKTRRTQRRRKEEDTKTENEDVAVVKSEPLVCVLEMIKRHEKFSLFERRLEKNQDLDRYKNTVKRHVDLETIQLRVQEGHYSSGTNTFFLDLVTLFSNATVFFSRRSPELRAAQQLRRLILDEMKTFGQAQSNTITQKSDSLPPNTPLSRPNSLSKHKPPILVCRKRSSIPVKPSNTTFSQKGDHKPIINDKKERPFSDVKPTLKPSSETDEEEPPKAKEKPVTGARSRRSNKNLSSNASNKKPPSNSTPKTGSSANKPAETPKLKNKAEGVSDKKKNNGAAGFLNRIKKNESVEVLRSGSGGGGGSSGSSKGGGGGGTNTKEPKKIVNNGKGEKGKERASRYNDVGGSGSGDKRNKNIESNSKRNVGRPPKKAAETVASTKRGRESSASGGKDKRPKKRSKK*