AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG009059


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009059 MtrunA17_Chr2g0308761 94.052 269 16 0 1 269 164 432 0.0 516
MsaG009059 MtrunA17_Chr2g0308431 89.011 91 8 1 155 245 1 89 9.91e-52 165
MsaG009059 MtrunA17_Chr4g0020831 95.238 84 4 0 155 238 1 84 2.75e-51 164
MsaG009059 MtrunA17_Chr1g0212691 27.638 199 124 4 4 191 245 434 9.53e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009059 AT1G74120.1 57.621 269 114 0 1 269 177 445 4.58e-113 333
MsaG009059 AT5G54180.1 29.730 185 125 4 10 191 290 472 2.16e-14 73.2
MsaG009059 AT1G62010.1 32.787 122 79 3 101 220 268 388 2.91e-13 69.7
MsaG009059 AT1G62120.1 29.167 120 84 1 102 220 291 410 5.24e-11 62.8
MsaG009059 AT1G62085.1 25.414 181 127 4 46 220 266 444 7.16e-11 62.4
MsaG009059 AT1G62085.2 25.414 181 127 4 46 220 266 444 7.16e-11 62.4
MsaG009059 AT5G23930.1 29.091 110 74 1 120 225 336 445 7.39e-11 62.4

Find 64 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAGGGATCCAAGGATTT+TGG 0.152438 2:-52422010 MsaT009059.1:CDS
ATTCATGAGCTGAGGGGTTT+GGG 0.198657 2:-52422063 MsaT009059.1:CDS
TAGATTGTTTCCTAGGGTTT+TGG 0.199228 2:-52422118 MsaT009059.1:CDS
CATGTTCATCACATCTTTAC+TGG 0.212061 2:+52421438 None:intergenic
GATTCATGAGCTGAGGGGTT+TGG 0.264040 2:-52422064 MsaT009059.1:CDS
AAGGTTGATTGGTTATGATT+TGG 0.292614 2:-52421797 MsaT009059.1:CDS
CAACACTATATTTCATCCTT+TGG 0.303808 2:+52421743 None:intergenic
AGGTCAGGGCGGAGGTTGTT+AGG 0.309694 2:-52422026 MsaT009059.1:CDS
AAATTCACACCCAAGTATTC+AGG 0.317076 2:+52421703 None:intergenic
TTGAAGGGAAAAGGTGCAAT+TGG 0.365701 2:-52421640 MsaT009059.1:CDS
GATGAACATGAAGGCTTTCA+TGG 0.369314 2:-52421425 MsaT009059.1:CDS
GAGTTGTTAGATTGTTTCCT+AGG 0.373935 2:-52422125 MsaT009059.1:CDS
TGAAGGGAAAAGGTGCAATT+GGG 0.398114 2:-52421639 MsaT009059.1:CDS
GGTGCAATTGGGTTTGAAGT+TGG 0.399622 2:-52421628 MsaT009059.1:CDS
ACTCTATCAATTTCATCCTT+AGG 0.408293 2:+52422144 None:intergenic
TGGGATTTGGTATTGAGGAT+AGG 0.409253 2:-52422098 MsaT009059.1:CDS
GAAAGCATCCCGTTGGACTT+TGG 0.413704 2:-52421492 MsaT009059.1:CDS
TCACATCTTTACTGGTTTGA+GGG 0.416518 2:+52421446 None:intergenic
TGGAAGGAACCAAGGTTGAT+TGG 0.421046 2:-52421808 MsaT009059.1:CDS
ATCACATCTTTACTGGTTTG+AGG 0.423102 2:+52421445 None:intergenic
AATTCGTAGGGATGCTTTGA+AGG 0.423395 2:-52421836 MsaT009059.1:CDS
TGTTGCAGAGTTTGAAATGC+AGG 0.431814 2:-52421951 MsaT009059.1:CDS
GCATGAAGTTCCTGAATACT+TGG 0.443861 2:-52421713 MsaT009059.1:CDS
GTTGATTGGTTATGATTTGG+AGG 0.446145 2:-52421794 MsaT009059.1:CDS
CCCCTCAGCTCATGAATCAA+TGG 0.448660 2:+52422069 None:intergenic
CAGCCGAGAGGTCAGGGCGG+AGG 0.450679 2:-52422034 MsaT009059.1:CDS
ATCTCCATTCCCAAAATCCT+TGG 0.454905 2:+52422000 None:intergenic
TACTCAATCACATTGTATCT+AGG 0.456654 2:+52421664 None:intergenic
TTATAAAATCTAAGCCTAGT+TGG 0.470603 2:+52421586 None:intergenic
CTCCGCCCTGACCTCTCGGC+TGG 0.473380 2:+52422035 None:intergenic
GGTCAGGGCGGAGGTTGTTA+GGG 0.481020 2:-52422025 MsaT009059.1:CDS
CAATGTGATTGAGTATTTGA+AGG 0.483329 2:-52421656 MsaT009059.1:CDS
AACATCTTCCAAAGTCCAAC+GGG 0.486105 2:+52421484 None:intergenic
ATTGAGTATTTGAAGGGAAA+AGG 0.490911 2:-52421649 MsaT009059.1:CDS
TGTGGAATTTGGGATTCCTA+AGG 0.495755 2:-52422160 MsaT009059.1:CDS
TTTGAGGGAACTTCGGTGGT+TGG 0.508881 2:+52421461 None:intergenic
TCATGGACTCGTTGGTGTAG+TGG 0.510176 2:-52421408 None:intergenic
AATGTGATTGAGTATTTGAA+GGG 0.522202 2:-52421655 MsaT009059.1:CDS
GTGGATTGTTTGTGCGGTCA+CGG 0.523503 2:-52421862 MsaT009059.1:CDS
TTCATGAGCTGAGGGGTTTG+GGG 0.523718 2:-52422062 MsaT009059.1:CDS
GCCATTGATTCATGAGCTGA+GGG 0.528936 2:-52422070 MsaT009059.1:CDS
TTACTGGTTTGAGGGAACTT+CGG 0.536810 2:+52421454 None:intergenic
AGTTGTTAGATTGTTTCCTA+GGG 0.538107 2:-52422124 MsaT009059.1:CDS
CGGAGGTTGTTAGGGATCCA+AGG 0.538817 2:-52422017 MsaT009059.1:CDS
CAACCTCCGCCCTGACCTCT+CGG 0.563699 2:+52422031 None:intergenic
ATACCAAATCCCAAAACCCT+AGG 0.565652 2:+52422108 None:intergenic
GCGGTCACGGTATAATTCGT+AGG 0.570160 2:-52421849 MsaT009059.1:CDS
GAAGGCTTTCATGGACTCGT+TGG 0.570328 2:-52421416 MsaT009059.1:CDS
CTGGTTTGAGGGAACTTCGG+TGG 0.572836 2:+52421457 None:intergenic
AAATCATAACCAATCAACCT+TGG 0.578600 2:+52421799 None:intergenic
TGCTTTGAAGGTGTTGTGGA+AGG 0.580553 2:-52421824 MsaT009059.1:CDS
CGGTCACGGTATAATTCGTA+GGG 0.581113 2:-52421848 MsaT009059.1:CDS
CATGAAGTTCCTGAATACTT+GGG 0.593187 2:-52421712 MsaT009059.1:CDS
AGCCATTGATTCATGAGCTG+AGG 0.597553 2:-52422071 MsaT009059.1:CDS
AAGACATTATCAAACCAACT+AGG 0.602970 2:-52421600 MsaT009059.1:CDS
GTTAAAAGAAAGCATCCCGT+TGG 0.604141 2:-52421499 MsaT009059.1:CDS
TAAAGATGTGATGAACATGA+AGG 0.609470 2:-52421434 MsaT009059.1:CDS
GGGATGCTTTGAAGGTGTTG+TGG 0.613256 2:-52421828 MsaT009059.1:CDS
GTTGCAGAGTTTGAAATGCA+GGG 0.619561 2:-52421950 MsaT009059.1:CDS
GAACATCTTCCAAAGTCCAA+CGG 0.624427 2:+52421483 None:intergenic
CTGAGAGTGGATTGTTTGTG+CGG 0.636549 2:-52421868 MsaT009059.1:CDS
CCATTGATTCATGAGCTGAG+GGG 0.690477 2:-52422069 MsaT009059.1:CDS
TTCCAGCCGAGAGGTCAGGG+CGG 0.702811 2:-52422037 MsaT009059.1:CDS
AGGTGTTGTGGAAGGAACCA+AGG 0.704640 2:-52421816 MsaT009059.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 52421411 52422220 52421411 ID=MsaG009059
Chr2 mRNA 52421411 52422220 52421411 ID=MsaT009059.1;Parent=MsaG009059
Chr2 exon 52421411 52422220 52421411 ID=MsaT009059.1.exon1;Parent=MsaT009059.1
Chr2 CDS 52421411 52422220 52421411 ID=cds.MsaT009059.1;Parent=MsaT009059.1
Gene Sequence

>MsaT009059.1

ATGGGGGAAACTCAAATTGTTAGTGTCATTGATTTTCTTGTGGAATTTGGGATTCCTAAGGATGAAATTGATAGAGTTGTTAGATTGTTTCCTAGGGTTTTGGGATTTGGTATTGAGGATAGGCTGAAGCCATTGATTCATGAGCTGAGGGGTTTGGGGTTTTCCAGCCGAGAGGTCAGGGCGGAGGTTGTTAGGGATCCAAGGATTTTGGGAATGGAGATTGGGGAATTTTCACGGTGTTTGAAGTTGTTGCAGAGTTTGAAATGCAGGGATGCAATTAAAGAGAAGATTTTTGGGGAGGGTTTGGTTAGGGCTTGTTTTGAAGTGAAGCTGAGAGTGGATTGTTTGTGCGGTCACGGTATAATTCGTAGGGATGCTTTGAAGGTGTTGTGGAAGGAACCAAGGTTGATTGGTTATGATTTGGAGGATATTGAGAAGAAGATTGAGTTTTTAATCCAAAGGATGAAATATAGTGTTGATTGTTTGCATGAAGTTCCTGAATACTTGGGTGTGAATTTTGAAAAACAGATTGTTCCTAGATACAATGTGATTGAGTATTTGAAGGGAAAAGGTGCAATTGGGTTTGAAGTTGGATTGAAAGACATTATCAAACCAACTAGGCTTAGATTTTATAATCTTTATGTCAAGCCTTACCCTGAATGTGAAAAAATATATGGCAGATTTTCAACAAAGGTTGAAGTTAAAAGAAAGCATCCCGTTGGACTTTGGAAGATGTTCCAACCACCGAAGTTCCCTCAAACCAGTAAAGATGTGATGAACATGAAGGCTTTCATGGACTCGTTGGTGTAG

Protein sequence

>MsaT009059.1

MGETQIVSVIDFLVEFGIPKDEIDRVVRLFPRVLGFGIEDRLKPLIHELRGLGFSSREVRAEVVRDPRILGMEIGEFSRCLKLLQSLKCRDAIKEKIFGEGLVRACFEVKLRVDCLCGHGIIRRDALKVLWKEPRLIGYDLEDIEKKIEFLIQRMKYSVDCLHEVPEYLGVNFEKQIVPRYNVIEYLKGKGAIGFEVGLKDIIKPTRLRFYNLYVKPYPECEKIYGRFSTKVEVKRKHPVGLWKMFQPPKFPQTSKDVMNMKAFMDSLV*