AlfalfaGEDB | MsaG009231

M. sativa ssp. caerulea / MsaG009231

KEGG: K08066
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0005737 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016602 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0090575 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009231	XP_039686567.1	95.849	265	11	0	1	265	1	265	5.82e-159	452

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009231	sp\|Q8LCG7\|NFYC2_ARATH	86.154	130	14	2	82	210	55	181	2.55e-77	235

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009231	tr\|I3TAY1\|I3TAY1_MEDTR	95.849	265	11	0	1	265	1	265	2.78e-159	452

TFs/TRs:

Gene ID	Type	Classification
MsaG009231	TF	NF-YC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009231	MtrunA17_Chr2g0310741	95.849	265	11	0	1	265	1	265	5.36e-163	452
MsaG009231	MtrunA17_Chr7g0272991	56.746	252	94	7	5	247	2	247	8.06e-79	238
MsaG009231	MtrunA17_Chr1g0191681	60.352	227	72	10	11	226	8	227	1.30e-72	223
MsaG009231	MtrunA17_Chr3g0132191	67.361	144	37	3	84	226	36	170	3.43e-62	195
MsaG009231	MtrunA17_Chr2g0288481	55.034	149	52	3	85	224	67	209	1.41e-52	171
MsaG009231	MtrunA17_Chr2g0318921	60.241	83	32	1	102	183	21	103	2.01e-30	110
MsaG009231	MtrunA17_Chr2g0318881	60.241	83	32	1	102	183	21	103	2.25e-30	110
MsaG009231	MtrunA17_Chr8g0376351	62.162	74	28	0	121	194	1	74	2.09e-26	99.0
MsaG009231	MtrunA17_Chr3g0081161	41.026	78	46	0	104	181	9	86	3.02e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009231	AT1G56170.1	86.154	130	14	2	82	210	55	181	2.59e-78	235
MsaG009231	AT1G56170.2	86.154	130	14	2	82	210	55	181	2.59e-78	235
MsaG009231	AT5G63470.2	58.128	203	66	5	41	234	20	212	1.83e-69	215
MsaG009231	AT5G63470.1	58.128	203	66	5	41	234	20	212	1.83e-69	215
MsaG009231	AT1G08970.3	71.528	144	38	3	85	226	62	204	7.12e-69	213
MsaG009231	AT1G08970.4	71.528	144	38	3	85	226	62	204	7.12e-69	213
MsaG009231	AT1G08970.2	71.528	144	38	3	85	226	62	204	7.12e-69	213
MsaG009231	AT1G08970.1	71.528	144	38	3	85	226	62	204	7.12e-69	213
MsaG009231	AT1G54830.2	69.388	147	33	3	85	226	52	191	4.24e-68	210
MsaG009231	AT1G54830.3	69.388	147	33	3	85	226	52	191	4.24e-68	210
MsaG009231	AT1G54830.1	69.388	147	33	3	85	226	52	191	4.24e-68	210
MsaG009231	AT3G48590.1	65.060	166	52	3	71	233	33	195	7.20e-68	210
MsaG009231	AT5G50480.1	47.879	165	70	4	81	243	35	185	1.38e-47	157
MsaG009231	AT5G27910.1	42.222	180	84	6	79	256	15	176	2.94e-40	138
MsaG009231	AT5G50490.1	39.429	175	93	4	72	244	8	171	1.06e-35	126
MsaG009231	AT5G50470.1	48.148	108	47	2	81	182	44	148	2.80e-30	113
MsaG009231	AT5G38140.1	45.000	100	51	2	82	180	48	144	2.24e-26	102

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGTGGTGGCTCCTGTTGC+TGG	0.253446	2:+55420222	None:intergenic
TAGAGTTGACTTTGCGATCT+TGG	0.263614	2:-55420562	MsaT009231.1:CDS
TTTGCTCAATTTCTTGCATT+TGG	0.264134	2:+55420714	None:intergenic
ACTGTTCCCATGTTGGCTTC+TGG	0.277594	2:-55420884	MsaT009231.1:CDS
TCTTCTTTCAATTCATCTCT+TGG	0.282962	2:+55420440	None:intergenic
TACGCGCAAGTGGGAGAGTA+TGG	0.334677	2:+55420675	None:intergenic
ATGCGGCTGGAGGCTGTGTT+GGG	0.338530	2:+55420837	None:intergenic
TATATTGCTGCATGATCAAC+TGG	0.341302	2:+55420302	None:intergenic
ATCAACAGCTTCAAATGTTC+TGG	0.346777	2:-55420742	MsaT009231.1:CDS
GTTGGTGTTGCTGCTGTTGA+TGG	0.352589	2:+55420765	None:intergenic
GGTGGAAATGCTGTGCTTGC+TGG	0.362971	2:+55420786	None:intergenic
GTCCCACCACAACATCCTTT+AGG	0.406073	2:-55420350	MsaT009231.1:CDS
AATGCGGCTGGAGGCTGTGT+TGG	0.408535	2:+55420836	None:intergenic
TGTGGTGGGACATAATAATA+TGG	0.412277	2:+55420362	None:intergenic
TCAGCTGGAGCACCGATTAT+CGG	0.426072	2:+55420389	None:intergenic
CAACATCCTTTAGGTCCTAC+AGG	0.429195	2:-55420341	MsaT009231.1:CDS
AAGCCTACTATTCCGATAAT+CGG	0.435007	2:-55420401	MsaT009231.1:CDS
AGTGAGAAGAATAGACCATT+TGG	0.449073	2:+55420912	None:intergenic
GTGCTTGCTGGTATGCGAAT+TGG	0.457941	2:+55420798	None:intergenic
CCACAGGAGGTCGAGGCTGC+TGG	0.460527	2:+55420273	None:intergenic
AAGTGCACACATCCAGCAAC+AGG	0.468963	2:-55420234	MsaT009231.1:CDS
TCAACAGCTTCAAATGTTCT+GGG	0.490507	2:-55420741	MsaT009231.1:CDS
GCTGGAGGCTGTGTTGGGGT+AGG	0.495800	2:+55420842	None:intergenic
CTCCTGTGGCTTTCATGCCA+TGG	0.498225	2:-55420259	MsaT009231.1:CDS
CGCACCAGGTACTAGCCAAA+TGG	0.499512	2:-55420927	MsaT009231.1:CDS
GCACCGATTATCGGAATAGT+AGG	0.501513	2:+55420398	None:intergenic
ATCATCCCTGTAGGACCTAA+AGG	0.501857	2:+55420335	None:intergenic
GAGCTTCTGCTGAAATCATC+CGG	0.503953	2:+55420621	None:intergenic
GAGTCTGTCTGTGGCTGTTG+TGG	0.506073	2:+55420206	None:intergenic
CTACCTGACTGTTCCCATGT+TGG	0.506876	2:-55420891	MsaT009231.1:CDS
AGGTCCTACAGGGATGATCA+TGG	0.513342	2:-55420330	MsaT009231.1:CDS
ATGAAAGCCACAGGAGGTCG+AGG	0.520693	2:+55420266	None:intergenic
AGAGATGAATTGAAAGAAGA+GGG	0.526767	2:-55420437	MsaT009231.1:CDS
TAATAATATGGAATATCAGC+TGG	0.527976	2:+55420374	None:intergenic
TCCTCTTGTTCTCTTCAGTG+TGG	0.538251	2:+55420537	None:intergenic
TCTGTCTGTGGCTGTTGTGG+TGG	0.546304	2:+55420209	None:intergenic
TGAAAGCCGATGAAGATGTC+CGG	0.548255	2:-55420640	MsaT009231.1:CDS
AAGCCAACATGGGAACAGTC+AGG	0.549822	2:+55420888	None:intergenic
GAATTGAAAGAAGAGGGGCT+TGG	0.553111	2:-55420431	MsaT009231.1:CDS
CTTGCTGGTATGCGAATTGG+TGG	0.555433	2:+55420801	None:intergenic
GGTGTTGCTGCTGTTGATGG+TGG	0.572770	2:+55420768	None:intergenic
AATCATCCGGACATCTTCAT+CGG	0.573019	2:+55420634	None:intergenic
AGGTGTGCCAGAAGCCAACA+TGG	0.576129	2:+55420877	None:intergenic
AAGAGATGAATTGAAAGAAG+AGG	0.576346	2:-55420438	MsaT009231.1:CDS
GCGGAATTAGAGAATGCGGC+TGG	0.581472	2:+55420824	None:intergenic
TAGACCATTTGGCTAGTACC+TGG	0.582406	2:+55420923	None:intergenic
TCTGCGATCGGAGTCGCACC+AGG	0.583461	2:-55420941	MsaT009231.1:CDS
GACCATGGCATGAAAGCCAC+AGG	0.584151	2:+55420257	None:intergenic
GAAAGAAGAGGGGCTTGGAA+TGG	0.586004	2:-55420426	MsaT009231.1:CDS
GACCTAAAGGATGTTGTGGT+GGG	0.590318	2:+55420348	None:intergenic
CCAGCAGCCTCGACCTCCTG+TGG	0.590845	2:-55420273	MsaT009231.1:CDS
GGGGTAGGAACAGCTACAGC+AGG	0.597736	2:+55420857	None:intergenic
GGTCCTACAGGGATGATCAT+GGG	0.598225	2:-55420329	MsaT009231.1:CDS
TTTCCCATGATCATCCCTGT+AGG	0.601110	2:+55420326	None:intergenic
ACATTTGAAGCTGTTGATGT+TGG	0.601353	2:+55420747	None:intergenic
TGCGGCTGGAGGCTGTGTTG+GGG	0.604131	2:+55420838	None:intergenic
TCCACACTGAAGAGAACAAG+AGG	0.607941	2:-55420538	MsaT009231.1:CDS
CAGCAGCAACAATCTGCGAT+CGG	0.614961	2:-55420953	MsaT009231.1:CDS
ATTTATTAAGAGTCTGTCTG+TGG	0.628344	2:+55420197	None:intergenic
GTAGGACCTAAAGGATGTTG+TGG	0.634993	2:+55420344	None:intergenic
GAATTAGAGAATGCGGCTGG+AGG	0.643197	2:+55420827	None:intergenic
GGACCTAAAGGATGTTGTGG+TGG	0.645250	2:+55420347	None:intergenic
TGGATGTGTGCACTTGACCA+TGG	0.646696	2:+55420242	None:intergenic
TTGCTGGTATGCGAATTGGT+GGG	0.649667	2:+55420802	None:intergenic
GTGGGCGGAATTAGAGAATG+CGG	0.665564	2:+55420820	None:intergenic
CTGGTATGCGAATTGGTGGG+CGG	0.667066	2:+55420805	None:intergenic
AACATCCTTTAGGTCCTACA+GGG	0.669689	2:-55420340	MsaT009231.1:CDS
CATGGCATGAAAGCCACAGG+AGG	0.683115	2:+55420260	None:intergenic
GAGATGAATTGAAAGAAGAG+GGG	0.683256	2:-55420436	MsaT009231.1:CDS
GGTGTGCCAGAAGCCAACAT+GGG	0.687320	2:+55420878	None:intergenic
ATATTGCTGCATGATCAACT+GGG	0.690297	2:+55420303	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	55420202	55420999	55420202	ID=MsaG009231
Chr2	mRNA	55420202	55420999	55420202	ID=MsaT009231.1;Parent=MsaG009231
Chr2	exon	55420202	55420999	55420202	ID=MsaT009231.1.exon1;Parent=MsaT009231.1
Chr2	CDS	55420202	55420999	55420202	ID=cds.MsaT009231.1;Parent=MsaT009231.1

Gene Sequence

>MsaT009231.1

ATGGATAATTCAGATCAGATTCAACAGCAGCAACAATCTGCGATCGGAGTCGCACCAGGTACTAGCCAAATGGTCTATTCTTCTCACTACCTGACTGTTCCCATGTTGGCTTCTGGCACACCTGCTGTAGCTGTTCCTACCCCAACACAGCCTCCAGCCGCATTCTCTAATTCCGCCCACCAATTCGCATACCAGCAAGCACAGCATTTCCACCATCAACAGCAGCAACACCAACATCAACAGCTTCAAATGTTCTGGGCAAACCAAATGCAAGAAATTGAGCAAACATTCGACTTTAAAAACCATACTCTCCCACTTGCGCGTATAAAGAAAATAATGAAAGCCGATGAAGATGTCCGGATGATTTCAGCAGAAGCTCCAGTCGTATTTGCAAAAGCATGTGAAATGTTTATATTAGAGTTGACTTTGCGATCTTGGATCCACACTGAAGAGAACAAGAGGAGAACGCTACAAAAGAATGACGTAGCAGCAGCTATTGCAAGAAATGATGTTTTTGATTTCTTGGTTGATATCATTCCAAGAGATGAATTGAAAGAAGAGGGGCTTGGAATGGCAAAGCCTACTATTCCGATAATCGGTGCTCCAGCTGATATTCCATATTATTATGTCCCACCACAACATCCTTTAGGTCCTACAGGGATGATCATGGGAAACCCAGTTGATCATGCAGCAATATATTCTAACCAGCAGCCTCGACCTCCTGTGGCTTTCATGCCATGGTCAAGTGCACACATCCAGCAACAGGAGCCACCACAACAGCCACAGACAGACTCTTAA

Protein sequence

>MsaT009231.1

MDNSDQIQQQQQSAIGVAPGTSQMVYSSHYLTVPMLASGTPAVAVPTPTQPPAAFSNSAHQFAYQQAQHFHHQQQQHQHQQLQMFWANQMQEIEQTFDFKNHTLPLARIKKIMKADEDVRMISAEAPVVFAKACEMFILELTLRSWIHTEENKRRTLQKNDVAAAIARNDVFDFLVDIIPRDELKEEGLGMAKPTIPIIGAPADIPYYYVPPQHPLGPTGMIMGNPVDHAAIYSNQQPRPPVAFMPWSSAHIQQQEPPQQPQTDS*