AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG009289


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009289 MtrunA17_Chr2g0311591 98.120 266 5 0 1 266 8 273 0.0 561
MsaG009289 MtrunA17_Chr8g0387241 77.419 62 14 0 149 210 211 272 1.42e-24 100
MsaG009289 MtrunA17_Chr5g0397361 53.846 91 39 1 136 226 149 236 1.08e-23 96.3
MsaG009289 MtrunA17_Chr1g0168401 45.192 104 47 1 117 210 263 366 2.43e-22 95.1
MsaG009289 MtrunA17_Chr1g0186171 56.164 73 32 0 144 216 297 369 1.88e-21 92.8
MsaG009289 MtrunA17_Chr2g0327621 74.074 54 14 0 154 207 146 199 3.13e-19 84.3
MsaG009289 MtrunA17_Chr4g0030421 59.677 62 25 0 148 209 20 81 4.37e-18 78.2
MsaG009289 MtrunA17_Chr3g0126721 60.000 50 20 0 159 208 226 275 5.51e-13 67.4
MsaG009289 MtrunA17_Chr1g0147111 53.061 49 23 0 159 207 228 276 9.24e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009289 AT3G12890.1 54.348 138 59 3 119 256 98 231 1.53e-43 148
MsaG009289 AT5G41380.1 75.806 62 15 0 149 210 201 262 5.57e-25 101
MsaG009289 AT1G63820.1 60.241 83 31 1 149 229 178 260 1.09e-23 97.8
MsaG009289 AT1G04500.4 42.857 126 55 3 100 224 237 346 4.75e-23 97.4
MsaG009289 AT1G04500.1 42.857 126 55 3 100 224 237 346 4.75e-23 97.4
MsaG009289 AT1G04500.3 42.857 126 55 3 100 224 228 337 4.84e-23 97.1
MsaG009289 AT2G33350.1 69.355 62 19 0 154 215 302 363 3.07e-22 95.5
MsaG009289 AT2G33350.2 69.355 62 19 0 154 215 301 362 3.14e-22 95.1
MsaG009289 AT2G33350.4 69.355 62 19 0 154 215 290 351 3.43e-22 95.1
MsaG009289 AT2G33350.5 69.355 62 19 0 154 215 310 371 4.39e-22 95.1
MsaG009289 AT5G59990.1 43.119 109 61 1 106 214 107 214 6.10e-15 72.8
MsaG009289 AT4G27900.1 53.061 49 23 0 159 207 210 258 6.12e-12 64.7
MsaG009289 AT4G27900.2 53.061 49 23 0 159 207 210 258 6.12e-12 64.7
MsaG009289 AT5G53420.2 55.102 49 22 0 159 207 135 183 7.42e-12 63.2
MsaG009289 AT5G53420.5 55.102 49 22 0 159 207 135 183 7.42e-12 63.2
MsaG009289 AT5G53420.4 55.102 49 22 0 159 207 135 183 7.42e-12 63.2
MsaG009289 AT5G53420.1 55.102 49 22 0 159 207 214 262 8.84e-12 64.3
MsaG009289 AT5G24930.1 30.460 174 102 6 47 214 186 346 7.97e-11 62.0

Find 52 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTTGGATTCCAAATT+TGG 0.102128 2:+56768054 MsaT009289.1:CDS
TCTTGTTGCTCAACCAAATT+TGG 0.128585 2:-56768067 None:intergenic
ATTGTGACGCTTCGGCTTCT+TGG 0.223720 2:+56768041 MsaT009289.1:CDS
ATGCCTGTTTATGATAATTA+TGG 0.229776 2:+56767992 MsaT009289.1:CDS
TTTATTATCAGATTGTAAAA+TGG 0.231726 2:+56768105 MsaT009289.1:CDS
CTAATGTTGCAAGAGAAAAT+TGG 0.300530 2:+56768260 MsaT009289.1:CDS
AATAATCAGCCACATATTAT+TGG 0.303040 2:+56768172 MsaT009289.1:CDS
TGCTGATTCATTTCAGTTCC+AGG 0.315116 2:+56769131 MsaT009289.1:CDS
CACATTAATGAAGATTTCTA+TGG 0.323076 2:+56769108 MsaT009289.1:CDS
TCAAGAGGCCATGGCAAGTT+TGG 0.324460 2:+56769749 MsaT009289.1:CDS
TACCTTCCTACCTTTATGTT+TGG 0.326979 2:-56768784 None:intergenic
GCAACAAGATTTCGCTGTTC+CGG 0.338361 2:+56768081 MsaT009289.1:CDS
TTATTATCAGATTGTAAAAT+GGG 0.383948 2:+56768106 MsaT009289.1:CDS
GCTCCATAATTATCATAAAC+AGG 0.394748 2:-56767995 None:intergenic
GAAAATATATAAGACCTTGA+TGG 0.404923 2:-56768878 None:intergenic
GTCACTTGATTGTGACGCTT+CGG 0.429884 2:+56768033 MsaT009289.1:CDS
TAATGTTGCAAGAGAAAATT+GGG 0.435066 2:+56768261 MsaT009289.1:CDS
CATATCATCTGGTGATGAAT+GGG 0.440400 2:-56769781 None:intergenic
TGATAGAAGAGTAAGAGTGA+GGG 0.443578 2:+56769011 MsaT009289.1:CDS
GAAGAGTAAGAGTGAGGGGA+AGG 0.444124 2:+56769016 MsaT009289.1:CDS
AATTAATTGCAGGGGATTCA+AGG 0.444134 2:+56768736 MsaT009289.1:intron
ACATATCATCTGGTGATGAA+TGG 0.448395 2:-56769782 None:intergenic
AGTTGAAGAGCCAAACATAA+AGG 0.450736 2:+56768774 MsaT009289.1:CDS
GCAACATTAGGATAAGTTGT+AGG 0.468600 2:-56768247 None:intergenic
TAATTAATTCACATATCATC+TGG 0.472556 2:-56769792 None:intergenic
CTGATAGAAGAGTAAGAGTG+AGG 0.485744 2:+56769010 MsaT009289.1:CDS
AGAAGATCAATGTTGTGGTT+TGG 0.497235 2:+56768207 MsaT009289.1:CDS
GGAGGGTTTCAGAATTTCAA+TGG 0.499215 2:+56768139 MsaT009289.1:CDS
TTACAATCTGATAATAAAGC+CGG 0.515082 2:-56768100 None:intergenic
GAAGGTACTCAGAAGAAGAA+AGG 0.519719 2:+56768800 MsaT009289.1:CDS
GATAGAAGAGTAAGAGTGAG+GGG 0.529042 2:+56769012 MsaT009289.1:CDS
ATAGAAGTGATGAGGGATTG+TGG 0.543919 2:-56767879 None:intergenic
GCAGTTAAAGAATGAAGAAG+AGG 0.550467 2:+56769719 MsaT009289.1:intron
AACTCATGACCAATAATATG+TGG 0.550988 2:-56768181 None:intergenic
TAGTTTGTATAGAAGTGATG+AGG 0.558490 2:-56767887 None:intergenic
TATGGACAATACTATGATGT+GGG 0.559347 2:+56767952 MsaT009289.1:CDS
AGTTTGTATAGAAGTGATGA+GGG 0.571794 2:-56767886 None:intergenic
TCTGATTTCACCACACAACA+AGG 0.572962 2:+56767914 MsaT009289.1:CDS
GAAGAGCCAAACATAAAGGT+AGG 0.576416 2:+56768778 MsaT009289.1:CDS
AGCCAAACATAAAGGTAGGA+AGG 0.578415 2:+56768782 MsaT009289.1:CDS
TAAAGAATGAAGAAGAGGAC+TGG 0.579395 2:+56769724 MsaT009289.1:CDS
GGACTGGCTTCAAGAGGCCA+TGG 0.582269 2:+56769740 MsaT009289.1:CDS
AAACTTCAACAAAACCATCA+AGG 0.586667 2:+56768864 MsaT009289.1:CDS
TTATGGACAATACTATGATG+TGG 0.594335 2:+56767951 MsaT009289.1:CDS
TAAATAGACCAAACTTGCCA+TGG 0.601398 2:-56769757 None:intergenic
ACATTAGGATAAGTTGTAGG+TGG 0.601914 2:-56768244 None:intergenic
CTGATTTCACCACACAACAA+GGG 0.621194 2:+56767915 MsaT009289.1:CDS
AGAAGAGGACTGGCTTCAAG+AGG 0.645775 2:+56769734 MsaT009289.1:CDS
GACATGTGAAGAAGATCACA+TGG 0.652465 2:+56769056 MsaT009289.1:CDS
TTTGTAGAAGATCAATGTTG+TGG 0.662640 2:+56768202 MsaT009289.1:CDS
TGAAGAAAACCCTTGTTGTG+TGG 0.693927 2:-56767924 None:intergenic
ATGGACAATACTATGATGTG+GGG 0.739532 2:+56767953 MsaT009289.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 56767869 56769806 56767869 ID=MsaG009289
Chr2 mRNA 56767869 56769806 56767869 ID=MsaT009289.1;Parent=MsaG009289
Chr2 exon 56767869 56768282 56767869 ID=MsaT009289.1.exon1;Parent=MsaT009289.1
Chr2 CDS 56767869 56768282 56767869 ID=cds.MsaT009289.1;Parent=MsaT009289.1
Chr2 exon 56768748 56768885 56768748 ID=MsaT009289.1.exon2;Parent=MsaT009289.1
Chr2 CDS 56768748 56768885 56768748 ID=cds.MsaT009289.1;Parent=MsaT009289.1
Chr2 exon 56768988 56769152 56768988 ID=MsaT009289.1.exon3;Parent=MsaT009289.1
Chr2 CDS 56768988 56769152 56768988 ID=cds.MsaT009289.1;Parent=MsaT009289.1
Chr2 exon 56769723 56769806 56769723 ID=MsaT009289.1.exon4;Parent=MsaT009289.1
Chr2 CDS 56769723 56769806 56769723 ID=cds.MsaT009289.1;Parent=MsaT009289.1
Gene Sequence

>MsaT009289.1

ATGGCTTCTTCCACAATCCCTCATCACTTCTATACAAACTATACTTCTGATTTCACCACACAACAAGGGTTTTCTTCAACAATTATGGACAATACTATGATGTGGGGTTGTCAAGAAAATATAATGCCTGTTTATGATAATTATGGAGCATTTGATCAAATTGTGTCACTTGATTGTGACGCTTCGGCTTCTTGGATTCCAAATTTGGTTGAGCAACAAGATTTCGCTGTTCCGGCTTTATTATCAGATTGTAAAATGGGATTTTATGGTGGAGGGTTTCAGAATTTCAATGGTAGATATAATAATAATCAGCCACATATTATTGGTCATGAGTTTGTAGAAGATCAATGTTGTGGTTTGGTTGAAGATGTTAAACCACCTACAACTTATCCTAATGTTGCAAGAGAAAATTGGGGGATTCAAGGTAATCAACTAGCAGCAGTTGAAGAGCCAAACATAAAGGTAGGAAGGTACTCAGAAGAAGAAAGGAAAGAAAGAATTCTTCGATATTTGAAGAAAAGAAATCAAAGAAACTTCAACAAAACCATCAAGTATGCATGTCGAAAAACTTTAGCTGATAGAAGAGTAAGAGTGAGGGGAAGGTTTGCAAGAAACAATGAGACATGTGAAGAAGATCACATGGCCACAAAAAAGCTTGAGAATCATCATGATCACATTAATGAAGATTTCTATGGTGCTGATTCATTTCAGTTCCAGTTAAAGAATGAAGAAGAGGACTGGCTTCAAGAGGCCATGGCAAGTTTGGTCTATTTATCCCATTCATCACCAGATGATATGTGA

Protein sequence

>MsaT009289.1

MASSTIPHHFYTNYTSDFTTQQGFSSTIMDNTMMWGCQENIMPVYDNYGAFDQIVSLDCDASASWIPNLVEQQDFAVPALLSDCKMGFYGGGFQNFNGRYNNNQPHIIGHEFVEDQCCGLVEDVKPPTTYPNVARENWGIQGNQLAAVEEPNIKVGRYSEEERKERILRYLKKRNQRNFNKTIKYACRKTLADRRVRVRGRFARNNETCEEDHMATKKLENHHDHINEDFYGADSFQFQLKNEEEDWLQEAMASLVYLSHSSPDDM*