AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG009299

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009299 MtrunA17_Chr2g0311661 92.661 109 8 0 3 111 66 174 1.09e-72 219
MsaG009299 MtrunA17_Chr2g0311651 90.826 109 10 0 3 111 67 175 1.04e-70 215
MsaG009299 MtrunA17_Chr5g0442771 70.000 100 17 3 2 89 49 147 9.20e-43 143
MsaG009299 MtrunA17_Chr5g0417001 73.494 83 20 1 12 94 67 147 7.67e-40 137
MsaG009299 MtrunA17_Chr8g0361731 68.605 86 25 1 12 97 67 150 8.22e-39 135
MsaG009299 MtrunA17_Chr6g0453131 57.843 102 34 2 10 105 66 164 3.67e-36 128
MsaG009299 MtrunA17_Chr6g0461661 45.385 130 68 2 12 140 71 198 1.73e-29 110
MsaG009299 MtrunA17_Chr7g0253011 61.538 78 30 0 11 88 69 146 1.18e-27 105
MsaG009299 MtrunA17_Chr4g0042561 61.039 77 26 1 12 88 62 134 2.26e-26 100
MsaG009299 MtrunA17_Chr5g0417791 58.974 78 28 1 11 88 55 128 7.68e-26 98.6
MsaG009299 MtrunA17_Chr8g0359921 54.369 103 34 4 8 104 59 154 8.06e-26 99.8
MsaG009299 MtrunA17_Chr2g0318081 61.538 78 26 1 11 88 68 141 1.04e-25 99.8
MsaG009299 MtrunA17_Chr4g0047841 57.143 77 31 1 12 88 68 142 1.16e-24 97.1
MsaG009299 MtrunA17_Chr6g0453451 55.682 88 34 2 12 99 73 155 1.23e-24 96.7
MsaG009299 MtrunA17_Chr4g0076731 58.228 79 30 1 11 89 106 181 1.27e-24 97.8
MsaG009299 MtrunA17_Chr4g0060931 50.538 93 42 1 12 104 70 158 2.74e-24 96.3
MsaG009299 MtrunA17_Chr7g0217721 51.724 87 38 1 12 98 72 154 3.11e-24 95.9
MsaG009299 MtrunA17_Chr7g0252971 52.439 82 34 1 12 88 61 142 3.11e-24 95.5
MsaG009299 MtrunA17_Chr3g0091921 50.000 94 43 1 11 104 68 157 3.50e-24 95.1
MsaG009299 MtrunA17_Chr7g0263931 48.837 86 42 1 12 97 64 147 1.20e-23 94.0
MsaG009299 MtrunA17_Chr2g0318101 54.545 77 33 1 12 88 68 142 1.31e-23 94.4
MsaG009299 MtrunA17_Chr3g0124761 55.814 86 29 3 7 88 51 131 1.32e-23 93.2
MsaG009299 MtrunA17_Chr2g0316971 57.143 77 31 1 12 88 76 150 3.41e-23 93.6
MsaG009299 MtrunA17_Chr8g0384721 57.143 77 28 2 12 88 60 131 3.89e-23 92.0
MsaG009299 MtrunA17_Chr2g0312321 53.488 86 32 2 7 88 53 134 4.01e-23 92.0
MsaG009299 MtrunA17_Chr5g0433781 50.526 95 35 2 8 94 57 147 4.34e-23 94.0
MsaG009299 MtrunA17_Chr4g0038751 45.000 100 50 2 7 102 59 157 5.07e-23 91.7
MsaG009299 MtrunA17_Chr3g0130731 57.692 78 28 2 11 88 59 131 6.65e-23 91.3
MsaG009299 MtrunA17_Chr7g0214071 50.000 86 40 2 7 91 59 142 6.80e-23 90.5
MsaG009299 MtrunA17_Chr2g0326361 53.750 80 35 1 12 91 67 144 9.77e-23 91.3
MsaG009299 MtrunA17_Chr7g0217711 48.864 88 41 1 11 98 71 154 1.53e-22 87.8
MsaG009299 MtrunA17_Chr5g0398971 51.163 86 37 2 12 96 63 144 1.72e-22 91.3
MsaG009299 MtrunA17_Chr6g0481091 50.617 81 36 1 8 88 57 133 1.75e-22 89.4
MsaG009299 MtrunA17_Chr8g0346001 47.727 88 42 1 11 98 70 153 1.81e-22 91.7
MsaG009299 MtrunA17_Chr6g0484441 51.163 86 34 2 7 88 49 130 1.92e-22 90.5
MsaG009299 MtrunA17_Chr3g0109821 53.086 81 34 1 8 88 57 133 2.25e-22 92.0
MsaG009299 MtrunA17_Chr3g0113001 53.846 78 32 1 11 88 17 90 2.99e-22 89.7
MsaG009299 MtrunA17_Chr3g0144271 45.833 96 50 1 12 107 77 170 3.38e-22 90.9
MsaG009299 MtrunA17_Chr1g0147421 47.872 94 43 2 8 97 73 164 6.20e-22 89.7
MsaG009299 MtrunA17_Chr4g0018391 50.649 77 34 1 12 88 63 135 6.57e-22 89.4
MsaG009299 MtrunA17_Chr2g0309331 39.344 122 55 2 10 115 64 182 7.43e-22 89.4
MsaG009299 MtrunA17_Chr2g0309891 50.617 81 36 1 8 88 57 133 9.80e-22 89.0
MsaG009299 MtrunA17_Chr8g0372121 49.383 81 37 1 14 94 64 140 1.70e-21 87.8
MsaG009299 MtrunA17_Chr7g0243371 47.674 86 43 1 6 91 11 94 1.72e-21 83.6
MsaG009299 MtrunA17_Chr3g0139331 46.809 94 44 2 8 97 56 147 2.11e-21 88.2
MsaG009299 MtrunA17_Chr4g0056541 51.948 77 33 1 12 88 63 135 4.30e-21 87.4
MsaG009299 MtrunA17_Chr1g0200561 51.316 76 33 1 12 87 75 146 6.12e-21 87.0
MsaG009299 MtrunA17_Chr8g0385991 42.857 112 49 4 2 103 45 151 7.00e-21 84.7
MsaG009299 MtrunA17_Chr5g0405211 49.367 79 36 1 12 90 64 138 1.57e-20 85.9
MsaG009299 MtrunA17_Chr5g0428991 51.316 76 33 1 12 87 80 151 5.26e-20 85.1
MsaG009299 MtrunA17_Chr8g0364791 52.000 75 32 1 14 88 63 133 1.35e-19 84.0
MsaG009299 MtrunA17_Chr3g0128151 51.316 76 33 1 12 87 77 148 1.69e-19 84.0
MsaG009299 MtrunA17_Chr8g0384201 49.333 75 34 1 14 88 63 133 2.17e-19 83.6
MsaG009299 MtrunA17_Chr1g0183741 52.000 75 34 1 14 88 65 137 3.09e-19 81.3
MsaG009299 MtrunA17_Chr3g0131911 42.424 99 47 2 2 90 45 143 3.23e-19 80.9
MsaG009299 MtrunA17_Chr4g0017921 47.619 84 40 1 11 94 63 142 4.46e-19 82.0
MsaG009299 MtrunA17_Chr7g0261411 52.000 75 34 1 14 88 65 137 8.47e-19 80.5
MsaG009299 MtrunA17_Chr1g0197361 45.455 88 44 1 14 101 66 149 9.36e-19 81.6
MsaG009299 MtrunA17_Chr1g0201021 46.154 78 38 1 11 88 73 146 1.24e-18 81.3
MsaG009299 MtrunA17_Chr5g0400461 44.048 84 44 1 8 88 57 140 4.01e-18 79.0
MsaG009299 MtrunA17_Chr3g0113031 48.052 77 36 1 23 99 4 76 1.03e-17 77.0
MsaG009299 MtrunA17_Chr2g0321741 36.522 115 60 4 11 116 45 155 2.43e-17 77.0
MsaG009299 MtrunA17_Chr3g0128111 46.053 76 37 1 12 87 97 168 8.48e-17 74.3
MsaG009299 MtrunA17_Chr8g0345671 45.570 79 40 2 8 86 63 138 3.18e-16 74.3
MsaG009299 MtrunA17_Chr4g0054791 41.053 95 53 2 8 102 57 148 5.17e-16 73.2
MsaG009299 MtrunA17_Chr3g0092671 44.578 83 42 1 14 96 63 141 5.31e-16 73.9
MsaG009299 MtrunA17_Chr3g0130101 47.945 73 34 1 16 88 66 134 1.36e-15 72.4
MsaG009299 MtrunA17_Chr1g0197351 45.333 75 37 1 12 86 97 167 1.07e-14 70.1
MsaG009299 MtrunA17_Chr2g0321631 41.026 78 43 1 10 87 61 135 2.53e-14 67.8
MsaG009299 MtrunA17_Chr8g0345721 37.500 96 57 2 8 103 116 208 3.40e-14 67.0
MsaG009299 MtrunA17_Chr4g0026141 46.154 78 36 3 12 87 62 135 3.42e-14 67.4
MsaG009299 MtrunA17_Chr8g0345691 45.333 75 38 2 12 86 68 139 4.91e-14 68.2
MsaG009299 MtrunA17_Chr7g0266701 40.000 75 42 1 13 87 64 135 9.37e-14 66.2
MsaG009299 MtrunA17_Chr8g0345741 43.038 79 42 2 8 86 43 118 1.45e-13 63.9
MsaG009299 MtrunA17_Chr8g0345711 37.778 90 53 2 8 97 64 150 1.32e-11 59.3
MsaG009299 MtrunA17_Chr8g0345731 33.333 96 60 2 8 101 76 169 2.82e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009299 AT2G43000.1 76.087 92 18 1 11 102 70 157 4.61e-44 146
MsaG009299 AT3G12910.1 76.190 84 19 1 11 94 74 156 7.43e-42 141
MsaG009299 AT2G43000.2 76.829 82 15 1 21 102 2 79 8.40e-39 130
MsaG009299 AT5G39820.1 69.880 83 23 1 12 94 73 153 4.06e-37 130
MsaG009299 AT1G26870.1 73.684 76 18 1 12 87 69 142 5.69e-36 128
MsaG009299 AT3G04070.2 54.545 88 39 1 9 95 60 147 9.05e-28 105
MsaG009299 AT3G04070.1 54.545 88 39 1 9 95 60 147 1.26e-27 105
MsaG009299 AT5G61430.2 54.839 93 38 1 12 104 6 94 6.35e-27 102
MsaG009299 AT2G02450.1 61.538 78 27 1 12 89 104 178 1.20e-26 103
MsaG009299 AT2G02450.2 61.538 78 27 1 12 89 104 178 1.26e-26 103
MsaG009299 AT5G61430.1 54.839 93 38 1 12 104 69 157 1.45e-26 102
MsaG009299 AT5G07680.2 53.763 93 39 1 12 104 56 144 1.77e-26 101
MsaG009299 AT5G07680.1 53.191 94 40 1 11 104 69 158 2.12e-26 101
MsaG009299 AT3G15500.1 49.138 116 47 3 11 118 66 177 2.98e-26 100
MsaG009299 AT1G69490.1 57.647 85 32 1 12 96 62 142 4.28e-26 99.8
MsaG009299 AT5G18270.2 62.025 79 25 2 11 88 73 147 5.27e-26 100
MsaG009299 AT5G13180.1 57.831 83 32 2 7 88 58 138 5.93e-26 99.0
MsaG009299 AT5G18270.1 62.025 79 25 2 11 88 73 147 6.09e-26 100
MsaG009299 AT4G27410.2 61.538 78 26 1 11 88 66 139 1.05e-25 99.4
MsaG009299 AT1G52890.1 56.667 90 33 2 11 98 66 151 1.49e-25 99.4
MsaG009299 AT4G27410.3 61.538 78 26 1 11 88 83 156 1.85e-25 99.0
MsaG009299 AT3G12977.2 59.211 76 27 1 13 88 19 90 2.62e-25 96.7
MsaG009299 AT5G62380.2 56.962 79 30 1 10 88 61 135 2.97e-25 99.0
MsaG009299 AT5G62380.1 56.962 79 30 1 10 88 61 135 2.97e-25 99.0
MsaG009299 AT3G12977.1 59.211 76 27 1 13 88 72 143 6.79e-25 96.7
MsaG009299 AT3G29035.1 57.692 78 29 1 11 88 76 149 1.17e-24 97.1
MsaG009299 AT5G39610.1 57.692 78 29 1 11 88 72 145 1.54e-24 95.9
MsaG009299 AT3G04060.1 60.759 79 26 2 11 88 72 146 1.57e-24 96.7
MsaG009299 AT3G15510.1 52.874 87 36 2 11 97 69 150 2.47e-24 96.7
MsaG009299 AT3G18400.1 56.962 79 30 1 12 90 58 132 4.15e-24 95.5
MsaG009299 AT2G17040.1 47.000 100 48 2 7 102 50 148 5.94e-24 94.4
MsaG009299 AT1G61110.1 46.729 107 49 2 11 117 68 166 9.42e-24 94.4
MsaG009299 AT5G08790.1 50.000 92 40 3 12 103 60 145 4.41e-23 92.0
MsaG009299 AT2G33480.1 47.959 98 43 3 7 103 59 149 4.82e-23 91.7
MsaG009299 AT1G01720.1 54.651 86 30 2 7 88 51 131 6.41e-23 91.7
MsaG009299 AT1G71930.2 53.247 77 32 1 12 88 65 137 7.73e-23 92.0
MsaG009299 AT1G71930.1 53.247 77 32 1 12 88 65 137 7.73e-23 92.0
MsaG009299 AT1G76420.1 53.846 78 34 1 11 88 74 149 8.92e-23 92.0
MsaG009299 AT5G63790.2 57.143 77 28 2 12 88 113 184 1.12e-22 91.7
MsaG009299 AT2G33480.3 48.387 93 41 2 11 103 7 92 1.16e-22 89.4
MsaG009299 AT5G63790.1 57.143 77 28 2 12 88 103 174 1.17e-22 91.3
MsaG009299 AT2G33480.2 47.059 102 46 3 7 107 59 153 1.20e-22 90.5
MsaG009299 AT2G46770.1 46.067 89 44 1 11 99 71 155 1.83e-22 91.7
MsaG009299 AT1G56010.1 53.947 76 31 1 13 88 7 78 3.20e-22 89.4
MsaG009299 AT4G35580.1 53.947 76 31 1 12 87 64 135 3.48e-22 91.7
MsaG009299 AT4G35580.3 53.947 76 31 1 12 87 64 135 4.47e-22 91.7
MsaG009299 AT4G35580.2 53.947 76 31 1 12 87 64 135 4.61e-22 91.7
MsaG009299 AT1G56010.2 53.947 76 31 1 13 88 74 145 4.66e-22 90.1
MsaG009299 AT1G65910.1 48.421 95 44 2 8 101 57 147 6.65e-22 91.3
MsaG009299 AT2G24430.2 51.765 85 39 1 13 97 70 152 8.70e-22 89.4
MsaG009299 AT2G24430.1 51.765 85 39 1 13 97 70 152 8.70e-22 89.4
MsaG009299 AT3G17730.1 49.383 81 37 1 8 88 57 133 1.34e-21 87.4
MsaG009299 AT1G77450.1 55.844 77 29 2 12 88 63 134 1.51e-21 87.4
MsaG009299 AT5G53950.1 51.163 86 36 2 7 88 61 144 2.07e-21 89.0
MsaG009299 AT3G61910.1 47.561 82 39 1 11 92 66 143 2.44e-21 88.2
MsaG009299 AT4G36160.2 50.617 81 36 1 14 94 72 148 2.50e-21 88.6
MsaG009299 AT4G36160.1 50.617 81 36 1 14 94 68 144 2.57e-21 88.6
MsaG009299 AT4G36160.3 50.617 81 36 1 14 94 68 144 2.57e-21 88.6
MsaG009299 AT4G10350.2 47.191 89 43 1 11 99 17 101 4.63e-21 87.0
MsaG009299 AT1G62700.1 48.750 80 37 1 12 91 63 138 6.24e-21 87.8
MsaG009299 AT1G62700.2 48.750 80 37 1 12 91 63 138 6.24e-21 87.8
MsaG009299 AT1G79580.3 50.633 79 36 1 10 88 71 146 7.09e-21 87.4
MsaG009299 AT1G79580.5 50.633 79 36 1 10 88 71 146 7.09e-21 87.4
MsaG009299 AT1G79580.4 50.633 79 36 1 10 88 71 146 7.09e-21 87.4
MsaG009299 AT1G79580.2 50.633 79 36 1 10 88 71 146 7.09e-21 87.4
MsaG009299 AT1G79580.1 50.633 79 36 1 10 88 71 146 7.09e-21 87.4
MsaG009299 AT2G18060.3 48.193 83 39 1 12 94 65 143 8.36e-21 87.0
MsaG009299 AT2G18060.1 48.193 83 39 1 12 94 65 143 8.36e-21 87.0
MsaG009299 AT4G10350.1 47.191 89 43 1 11 99 64 148 8.51e-21 86.7
MsaG009299 AT3G03200.1 48.810 84 39 1 8 91 57 136 1.52e-20 87.0
MsaG009299 AT1G52880.1 58.442 77 32 0 12 88 70 146 2.36e-20 85.5
MsaG009299 AT5G46590.2 46.914 81 40 1 8 88 57 134 2.61e-20 84.7
MsaG009299 AT3G15170.1 53.247 77 34 1 12 88 73 147 3.38e-20 84.7
MsaG009299 AT5G46590.1 46.914 81 40 1 8 88 57 134 3.58e-20 84.3
MsaG009299 AT1G12260.2 49.351 77 35 1 12 88 63 135 3.73e-20 85.1
MsaG009299 AT1G12260.1 49.351 77 35 1 12 88 63 135 3.93e-20 85.5
MsaG009299 AT5G66300.1 50.667 75 33 1 12 86 68 138 4.73e-20 84.3
MsaG009299 AT1G33280.1 49.398 83 38 1 11 93 63 141 5.56e-20 84.3
MsaG009299 AT1G33060.2 50.000 76 34 1 12 87 79 150 6.63e-20 85.5
MsaG009299 AT1G34190.1 48.718 78 36 1 11 88 70 143 6.77e-20 85.5
MsaG009299 AT1G33060.1 50.000 76 34 1 12 87 79 150 6.89e-20 85.5
MsaG009299 AT5G17260.1 49.383 81 37 1 8 88 57 133 7.03e-20 85.1
MsaG009299 AT1G32510.1 39.806 103 59 1 8 107 57 159 1.91e-19 82.4
MsaG009299 AT1G34180.1 48.718 78 36 1 11 88 70 143 2.09e-19 84.0
MsaG009299 AT1G34180.4 48.718 78 36 1 11 88 82 155 2.69e-19 83.6
MsaG009299 AT1G34180.3 44.565 92 41 2 3 88 56 143 3.43e-19 83.2
MsaG009299 AT1G34180.2 49.351 77 35 1 12 88 83 155 5.43e-19 82.8
MsaG009299 AT1G32770.1 48.810 84 33 2 11 91 71 147 9.11e-19 81.3
MsaG009299 AT4G17980.1 45.679 81 42 1 8 88 57 135 1.30e-18 79.7
MsaG009299 AT1G54330.1 45.745 94 47 1 8 101 57 146 1.78e-18 80.1
MsaG009299 AT4G17980.2 45.679 81 42 1 8 88 57 135 1.86e-18 79.7
MsaG009299 AT4G28530.1 52.632 76 34 1 13 88 80 153 2.09e-18 80.5
MsaG009299 AT3G10500.1 49.333 75 34 1 14 88 66 136 2.10e-18 80.9
MsaG009299 AT5G09330.5 42.169 83 44 1 16 98 65 143 2.25e-18 80.9
MsaG009299 AT5G09330.2 42.169 83 44 1 16 98 65 143 2.25e-18 80.9
MsaG009299 AT5G09330.4 42.169 83 44 1 16 98 65 143 2.25e-18 80.9
MsaG009299 AT5G09330.3 42.169 83 44 1 16 98 65 143 2.25e-18 80.9
MsaG009299 AT5G09330.1 42.169 83 44 1 16 98 65 143 2.25e-18 80.9
MsaG009299 AT5G24590.2 48.684 76 36 1 12 87 68 140 1.31e-17 78.6
MsaG009299 AT5G04410.1 48.000 75 35 1 14 88 66 136 2.94e-17 77.8
MsaG009299 AT2G27300.1 44.737 76 38 1 11 86 67 138 3.76e-17 76.6
MsaG009299 AT3G49530.1 45.000 80 41 2 8 87 64 140 7.25e-17 76.6
MsaG009299 AT3G10480.3 45.455 77 38 1 12 88 115 187 7.74e-17 76.6
MsaG009299 AT5G22290.1 42.500 80 42 1 7 86 70 145 8.95e-17 75.9
MsaG009299 AT3G49530.2 45.000 80 41 2 8 87 64 140 1.77e-16 75.5
MsaG009299 AT3G10480.1 45.455 77 38 1 12 88 82 154 2.22e-16 75.1
MsaG009299 AT3G10480.2 45.455 77 38 1 12 88 82 154 2.37e-16 75.1
MsaG009299 AT3G44290.2 39.286 84 47 1 3 86 59 138 2.44e-16 73.2
MsaG009299 AT3G44290.1 41.250 80 43 1 7 86 63 138 5.69e-16 73.6
MsaG009299 AT3G10490.2 44.156 77 39 1 12 88 82 154 6.69e-16 73.9
MsaG009299 AT3G10490.1 44.156 77 39 1 12 88 82 154 1.72e-15 71.2
MsaG009299 AT4G01540.1 41.176 85 49 1 4 88 53 136 1.80e-15 72.4
MsaG009299 AT4G01540.4 40.230 87 51 1 2 88 51 136 2.94e-15 71.6
MsaG009299 AT5G64060.1 38.095 84 48 1 16 99 65 144 3.00e-15 71.6
MsaG009299 AT4G01540.2 40.230 87 51 1 2 88 51 136 3.84e-15 71.6
MsaG009299 AT4G01540.3 40.230 87 51 1 2 88 51 136 4.43e-15 71.2
MsaG009299 AT4G01520.1 39.080 87 52 1 2 88 51 136 6.28e-15 70.5
MsaG009299 AT3G44350.1 39.080 87 50 1 10 96 61 144 1.35e-14 67.4
MsaG009299 AT4G01550.1 43.836 73 40 1 16 88 64 135 2.36e-14 69.3
MsaG009299 AT2G18060.2 51.724 58 24 1 37 94 2 55 3.11e-14 68.2
MsaG009299 AT3G44350.2 39.080 87 50 1 10 96 61 144 3.67e-14 67.4
MsaG009299 AT5G22380.1 38.947 95 54 2 3 96 55 146 5.08e-14 67.0
MsaG009299 AT5G04395.1 41.558 77 44 1 12 88 94 169 1.97e-13 64.7
MsaG009299 AT1G32870.2 42.857 77 38 2 12 88 65 135 2.56e-13 66.6
MsaG009299 AT1G32870.1 42.857 77 38 2 12 88 65 135 2.56e-13 66.6
MsaG009299 AT1G32870.3 42.857 77 38 2 12 88 101 171 6.36e-13 65.5

Find 21 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGTCACTGGCTCTGGATTC+TGG 0.244802 2:+56924431 MsaT009299.1:CDS
CCAGAGCCAGTGACTCTATT+TGG 0.333205 2:-56924424 None:intergenic
ATGCATCATCCAATCAGTTT+TGG 0.355638 2:-56924563 None:intergenic
GCCAAAGAGGAAGAAAATAT+AGG 0.410785 2:+56924389 MsaT009299.1:CDS
CCAAATAGAGTCACTGGCTC+TGG 0.412912 2:+56924424 MsaT009299.1:CDS
TCTGGATTCTGGAAAGCAAC+TGG 0.467422 2:+56924442 MsaT009299.1:CDS
GACAAAGCAATATACTCAAA+TGG 0.469362 2:+56924469 MsaT009299.1:CDS
GTAAAGGCACCAAAACTGAT+TGG 0.491861 2:+56924554 MsaT009299.1:CDS
GTTTACTATCGTGGTAGTGC+TGG 0.494944 2:+56924532 MsaT009299.1:CDS
AAATATACCAGGAGCAGAGA+AGG 0.498611 2:+56924351 MsaT009299.1:CDS
TAACCTTCCTTCTCTGCTCC+TGG 0.500000 2:-56924358 None:intergenic
ATATACTCAAATGGAGGAGA+AGG 0.533042 2:+56924478 MsaT009299.1:CDS
AAAACACTTGTTTACTATCG+TGG 0.541680 2:+56924523 MsaT009299.1:CDS
TATCGTGGTAGTGCTGGTAA+AGG 0.553822 2:+56924538 MsaT009299.1:CDS
GTTAGACCAAATAGAGTCAC+TGG 0.561770 2:+56924418 MsaT009299.1:CDS
TAACGTCAAATGGTCGTGAA+AGG 0.578043 2:+56921575 None:intergenic
GGAGAAGGAAATGATTGTGT+TGG 0.583944 2:+56924493 MsaT009299.1:CDS
ATCAGCATCATTCTTAGCAT+AGG 0.603706 2:-56924629 None:intergenic
ATACCAGGAGCAGAGAAGGA+AGG 0.629648 2:+56924355 MsaT009299.1:CDS
AAAGCAATATACTCAAATGG+AGG 0.648134 2:+56924472 MsaT009299.1:CDS
TATGCAGAAGTAAATATACC+AGG 0.662988 2:+56924340 MsaT009299.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 56921584 56924780 56921584 ID=MsaG009299
Chr2 mRNA 56921584 56924780 56921584 ID=MsaT009299.1;Parent=MsaG009299
Chr2 exon 56921584 56921596 56921584 ID=MsaT009299.1.exon1;Parent=MsaT009299.1
Chr2 CDS 56921584 56921596 56921584 ID=cds.MsaT009299.1;Parent=MsaT009299.1
Chr2 exon 56924347 56924780 56924347 ID=MsaT009299.1.exon2;Parent=MsaT009299.1
Chr2 CDS 56924347 56924780 56924347 ID=cds.MsaT009299.1;Parent=MsaT009299.1
Gene Sequence

>MsaT009299.1

ATGGTCGTGAAAGAAGTAAATATACCAGGAGCAGAGAAGGAAGGTTACTTTTTTTGCCAAAGAGGAAGAAAATATAGGAACAGCGTTAGACCAAATAGAGTCACTGGCTCTGGATTCTGGAAAGCAACTGGAATAGACAAAGCAATATACTCAAATGGAGGAGAAGGAAATGATTGTGTTGGACTCAAAAAAACACTTGTTTACTATCGTGGTAGTGCTGGTAAAGGCACCAAAACTGATTGGATGATGCATGAGTTTCGTCTTCCATCAACCATTGATGCTAAAACAAACATTTCCTATGCTAAGAATGATGCTGATTGTGCTCATGAAGCTGTAAATAACTTACATATGAGCTATACCAAATTTTTACAAAATTTTTATGCTACAATATTTGTTCACAAGCACATTATTAATTTCTCTCTTACTTTTCAGTCACATTTTTCTTGA
Protein sequence

>MsaT009299.1

MVVKEVNIPGAEKEGYFFCQRGRKYRNSVRPNRVTGSGFWKATGIDKAIYSNGGEGNDCVGLKKTLVYYRGSAGKGTKTDWMMHEFRLPSTIDAKTNISYAKNDADCAHEAVNNLHMSYTKFLQNFYATIFVHKHIINFSLTFQSHFS*