AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG009303

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009303 MtrunA17_Chr2g0311651 58.654 312 52 2 1 235 1 312 1.86e-121 347
MsaG009303 MtrunA17_Chr2g0311661 60.129 311 28 4 1 235 1 291 3.54e-120 343
MsaG009303 MtrunA17_Chr5g0442771 44.444 243 104 8 15 231 63 300 4.20e-55 178
MsaG009303 MtrunA17_Chr5g0417001 55.357 112 26 2 20 109 63 172 8.60e-36 130
MsaG009303 MtrunA17_Chr8g0361731 37.306 193 86 5 20 183 63 249 2.41e-34 126
MsaG009303 MtrunA17_Chr6g0453131 51.327 113 29 2 20 109 64 173 4.01e-33 123
MsaG009303 MtrunA17_Chr2g0318081 51.818 110 35 2 23 118 68 173 3.13e-29 112
MsaG009303 MtrunA17_Chr8g0359921 50.893 112 35 2 23 118 66 173 3.35e-29 111
MsaG009303 MtrunA17_Chr2g0312321 33.673 196 99 5 1 181 40 219 1.23e-26 104
MsaG009303 MtrunA17_Chr5g0417791 47.619 105 33 2 23 109 55 155 2.52e-25 100
MsaG009303 MtrunA17_Chr4g0042561 48.077 104 32 2 24 109 62 161 4.03e-25 99.8
MsaG009303 MtrunA17_Chr7g0253011 45.872 109 43 1 23 115 69 177 5.01e-25 101
MsaG009303 MtrunA17_Chr2g0318101 39.200 125 49 2 18 117 62 184 5.95e-25 100
MsaG009303 MtrunA17_Chr7g0252971 42.241 116 44 3 18 110 55 170 2.00e-24 99.4
MsaG009303 MtrunA17_Chr3g0124761 44.000 125 44 4 13 116 45 164 2.29e-24 98.2
MsaG009303 MtrunA17_Chr3g0091921 44.037 109 41 2 23 115 68 172 4.86e-24 97.4
MsaG009303 MtrunA17_Chr6g0461661 44.828 116 46 2 24 123 71 184 5.70e-24 98.6
MsaG009303 MtrunA17_Chr6g0484441 42.857 105 44 2 23 115 57 157 1.02e-23 97.1
MsaG009303 MtrunA17_Chr3g0139331 33.166 199 103 5 23 202 63 250 1.07e-23 97.4
MsaG009303 MtrunA17_Chr7g0263931 41.818 110 44 2 23 114 63 170 1.37e-23 96.7
MsaG009303 MtrunA17_Chr8g0384721 45.714 105 34 3 23 109 59 158 1.91e-23 95.5
MsaG009303 MtrunA17_Chr4g0047841 55.000 80 34 1 23 102 67 144 1.93e-23 96.7
MsaG009303 MtrunA17_Chr3g0130731 47.115 104 33 3 23 109 59 157 2.30e-23 95.5
MsaG009303 MtrunA17_Chr4g0076731 41.221 131 61 3 18 135 101 228 3.12e-23 97.1
MsaG009303 MtrunA17_Chr3g0113001 31.081 222 116 7 23 213 17 232 3.45e-23 95.1
MsaG009303 MtrunA17_Chr4g0060931 42.202 109 43 2 23 115 69 173 5.12e-23 95.5
MsaG009303 MtrunA17_Chr4g0018391 41.071 112 48 3 15 112 52 159 5.74e-23 95.5
MsaG009303 MtrunA17_Chr6g0453451 43.119 109 42 2 23 115 72 176 7.58e-23 94.7
MsaG009303 MtrunA17_Chr5g0398971 33.333 150 77 4 24 159 63 203 1.72e-22 94.0
MsaG009303 MtrunA17_Chr2g0309331 33.702 181 90 6 21 187 63 227 3.91e-22 93.2
MsaG009303 MtrunA17_Chr2g0326361 44.118 102 37 2 23 106 66 165 4.18e-22 92.8
MsaG009303 MtrunA17_Chr7g0214071 44.231 104 44 2 24 115 65 166 5.43e-22 91.3
MsaG009303 MtrunA17_Chr4g0056541 41.121 107 47 2 28 122 67 169 1.11e-21 92.0
MsaG009303 MtrunA17_Chr2g0309891 29.741 232 127 7 20 234 57 269 1.82e-21 91.3
MsaG009303 MtrunA17_Chr2g0316971 40.496 121 53 3 6 110 59 176 2.76e-21 91.3
MsaG009303 MtrunA17_Chr1g0147421 42.718 103 45 2 25 115 82 182 3.11e-21 90.9
MsaG009303 MtrunA17_Chr6g0481091 39.640 111 49 2 14 110 51 157 3.93e-21 88.6
MsaG009303 MtrunA17_Chr8g0372121 42.424 99 41 2 26 112 64 158 4.50e-21 89.7
MsaG009303 MtrunA17_Chr8g0346001 41.905 105 34 2 28 109 75 175 6.60e-21 90.5
MsaG009303 MtrunA17_Chr3g0109821 41.739 115 48 2 20 119 57 167 8.96e-21 90.5
MsaG009303 MtrunA17_Chr1g0200561 43.878 98 39 2 24 109 75 168 1.29e-20 89.0
MsaG009303 MtrunA17_Chr1g0183741 31.579 171 99 4 6 166 46 208 1.33e-20 87.8
MsaG009303 MtrunA17_Chr5g0433781 42.857 105 42 2 20 110 57 157 4.37e-20 88.6
MsaG009303 MtrunA17_Chr4g0038751 37.594 133 63 4 11 124 51 182 6.08e-20 86.3
MsaG009303 MtrunA17_Chr4g0017921 39.316 117 54 3 6 109 44 156 1.35e-19 86.3
MsaG009303 MtrunA17_Chr7g0217721 50.000 78 35 1 23 100 71 144 1.47e-19 85.9
MsaG009303 MtrunA17_Chr5g0405211 40.404 99 43 2 26 112 66 160 1.65e-19 85.9
MsaG009303 MtrunA17_Chr3g0144271 44.211 95 50 2 6 100 60 151 3.74e-19 85.5
MsaG009303 MtrunA17_Chr7g0261411 45.652 92 38 2 26 107 65 154 6.48e-19 83.6
MsaG009303 MtrunA17_Chr3g0128151 41.284 109 46 2 16 110 69 173 1.84e-18 84.0
MsaG009303 MtrunA17_Chr7g0217711 44.211 95 48 2 6 100 55 144 2.28e-18 79.7
MsaG009303 MtrunA17_Chr5g0428991 40.000 110 48 2 14 109 70 175 6.12e-18 82.4
MsaG009303 MtrunA17_Chr7g0243371 48.052 77 38 1 24 100 17 91 1.42e-17 75.9
MsaG009303 MtrunA17_Chr3g0131911 33.333 159 65 4 23 157 64 205 2.25e-17 78.2
MsaG009303 MtrunA17_Chr1g0197361 40.777 103 44 2 26 115 66 164 2.50e-17 80.5
MsaG009303 MtrunA17_Chr8g0385991 39.815 108 44 2 24 112 65 170 2.59e-17 77.8
MsaG009303 MtrunA17_Chr3g0113031 29.469 207 108 7 35 209 4 204 3.43e-17 78.2
MsaG009303 MtrunA17_Chr1g0197351 35.897 117 51 2 14 110 87 199 3.59e-16 77.0
MsaG009303 MtrunA17_Chr2g0321741 35.526 152 76 6 23 174 45 174 4.79e-16 76.3
MsaG009303 MtrunA17_Chr1g0201021 39.583 96 54 1 5 100 55 146 1.43e-15 75.5
MsaG009303 MtrunA17_Chr8g0364791 34.545 110 54 2 14 109 51 156 1.55e-15 75.1
MsaG009303 MtrunA17_Chr3g0128111 38.614 101 44 2 24 110 97 193 2.32e-15 72.8
MsaG009303 MtrunA17_Chr3g0092671 38.835 103 46 2 26 115 63 161 3.40e-15 74.3
MsaG009303 MtrunA17_Chr5g0400461 33.333 120 66 2 5 110 42 161 3.80e-15 73.6
MsaG009303 MtrunA17_Chr8g0384201 35.714 98 45 2 26 109 63 156 1.11e-14 72.8
MsaG009303 MtrunA17_Chr3g0130101 37.500 96 42 2 28 109 66 157 3.22e-14 70.9
MsaG009303 MtrunA17_Chr8g0345671 38.542 96 47 3 20 106 63 155 4.42e-14 70.9
MsaG009303 MtrunA17_Chr4g0026141 37.864 103 41 4 24 107 62 160 2.39e-13 67.4
MsaG009303 MtrunA17_Chr2g0321631 41.026 78 43 1 22 99 61 135 4.22e-13 66.6
MsaG009303 MtrunA17_Chr7g0266701 32.479 117 56 3 17 114 57 169 4.39e-13 66.6
MsaG009303 MtrunA17_Chr4g0054791 34.615 104 53 2 26 115 63 165 1.18e-12 66.2
MsaG009303 MtrunA17_Chr8g0345691 38.144 97 48 3 20 107 64 157 2.13e-12 65.9
MsaG009303 MtrunA17_Chr8g0345721 40.476 84 47 2 20 103 116 196 3.05e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009303 AT3G12910.1 44.628 242 80 9 23 229 74 296 2.85e-50 166
MsaG009303 AT2G43000.1 71.963 107 11 2 23 114 70 172 5.03e-46 154
MsaG009303 AT2G43000.2 73.196 97 7 2 33 114 2 94 1.87e-42 143
MsaG009303 AT1G26870.1 55.455 110 24 2 23 109 68 175 5.67e-35 129
MsaG009303 AT5G39820.1 42.593 162 50 4 20 140 69 228 3.76e-34 125
MsaG009303 AT1G52890.1 47.581 124 46 3 23 132 66 184 7.68e-28 108
MsaG009303 AT3G15500.1 50.926 108 35 2 23 116 66 169 1.43e-27 107
MsaG009303 AT3G12977.2 38.122 181 70 8 16 179 12 167 5.55e-27 103
MsaG009303 AT4G27410.2 49.074 108 37 2 23 116 66 169 7.84e-27 105
MsaG009303 AT4G27410.3 49.074 108 37 2 23 116 83 186 1.68e-26 104
MsaG009303 AT3G12977.1 37.705 183 72 8 14 179 63 220 3.68e-26 103
MsaG009303 AT3G04070.2 44.348 115 42 2 18 110 57 171 4.15e-26 104
MsaG009303 AT5G08790.1 35.795 176 76 7 11 162 48 210 5.82e-26 102
MsaG009303 AT1G61110.1 43.802 121 39 2 23 117 68 185 7.14e-26 103
MsaG009303 AT3G15510.1 41.463 123 45 2 17 114 63 183 1.06e-25 103
MsaG009303 AT3G18400.1 43.243 111 46 2 18 115 52 158 4.64e-25 100
MsaG009303 AT2G02450.2 50.000 104 37 2 18 109 98 198 5.22e-25 102
MsaG009303 AT2G02450.1 50.000 104 37 2 18 109 98 198 5.40e-25 101
MsaG009303 AT5G62380.2 45.192 104 41 2 21 112 60 159 5.76e-25 101
MsaG009303 AT5G62380.1 45.192 104 41 2 21 112 60 159 5.76e-25 101
MsaG009303 AT5G13180.1 51.546 97 32 2 24 107 64 158 5.85e-25 99.4
MsaG009303 AT5G07680.2 31.401 207 120 4 23 213 55 255 8.05e-25 100
MsaG009303 AT5G61430.2 45.536 112 40 3 23 118 5 111 8.06e-25 99.4
MsaG009303 AT1G69490.1 47.619 105 33 2 23 109 61 161 8.50e-25 99.4
MsaG009303 AT5G63790.1 36.735 147 68 4 6 133 86 226 9.74e-25 100
MsaG009303 AT5G63790.2 36.735 147 68 4 6 133 96 236 1.01e-24 100
MsaG009303 AT5G07680.1 31.401 207 120 4 23 213 69 269 1.01e-24 100
MsaG009303 AT1G71930.2 35.088 171 88 4 24 181 65 225 1.89e-24 99.4
MsaG009303 AT1G71930.1 35.088 171 88 4 24 181 65 225 1.89e-24 99.4
MsaG009303 AT5G18270.2 48.571 105 33 3 23 110 73 173 2.02e-24 99.4
MsaG009303 AT5G61430.1 45.536 112 40 3 23 118 68 174 2.44e-24 99.4
MsaG009303 AT5G18270.1 48.571 105 33 3 23 110 73 173 2.46e-24 99.4
MsaG009303 AT1G01720.1 45.217 115 39 3 23 118 59 168 4.77e-24 97.8
MsaG009303 AT3G04060.1 43.200 125 45 4 23 125 72 192 5.68e-24 98.2
MsaG009303 AT2G24430.2 40.800 125 58 3 6 117 52 173 6.08e-24 97.8
MsaG009303 AT2G24430.1 40.800 125 58 3 6 117 52 173 6.08e-24 97.8
MsaG009303 AT3G04070.1 53.571 84 38 1 18 100 57 140 8.44e-24 98.2
MsaG009303 AT1G56010.1 35.028 177 86 6 18 176 2 167 4.18e-23 94.4
MsaG009303 AT1G56010.2 32.804 189 99 6 6 176 56 234 4.58e-23 95.5
MsaG009303 AT1G76420.1 41.026 117 53 3 6 109 58 171 1.03e-22 94.7
MsaG009303 AT5G39610.1 43.119 109 42 2 23 115 72 176 1.19e-22 94.0
MsaG009303 AT1G77450.1 43.750 112 40 4 23 116 62 168 4.23e-22 91.7
MsaG009303 AT1G52880.1 44.915 118 43 1 17 112 63 180 8.01e-22 92.0
MsaG009303 AT2G33480.3 44.231 104 40 2 23 112 7 106 1.61e-21 89.4
MsaG009303 AT2G33480.2 43.636 110 45 3 16 112 56 161 1.86e-21 90.1
MsaG009303 AT1G79580.3 43.000 100 43 2 21 109 70 166 2.64e-21 91.3
MsaG009303 AT1G79580.5 43.000 100 43 2 21 109 70 166 2.64e-21 91.3
MsaG009303 AT1G79580.4 43.000 100 43 2 21 109 70 166 2.64e-21 91.3
MsaG009303 AT1G79580.2 43.000 100 43 2 21 109 70 166 2.64e-21 91.3
MsaG009303 AT1G79580.1 43.000 100 43 2 21 109 70 166 2.64e-21 91.3
MsaG009303 AT2G17040.1 29.493 217 126 7 23 225 58 261 2.98e-21 89.7
MsaG009303 AT1G62700.1 40.594 101 44 2 24 112 63 159 3.14e-21 91.7
MsaG009303 AT1G62700.2 40.594 101 44 2 24 112 63 159 3.14e-21 91.7
MsaG009303 AT2G33480.1 42.857 112 45 3 16 112 56 163 4.07e-21 89.4
MsaG009303 AT3G29035.1 53.846 78 32 1 23 100 76 149 6.52e-21 89.7
MsaG009303 AT1G12260.1 39.216 102 46 2 28 117 67 164 8.96e-21 90.1
MsaG009303 AT1G12260.2 39.216 102 46 2 28 117 67 164 9.35e-21 89.7
MsaG009303 AT5G66300.1 40.909 110 45 3 24 117 68 173 1.42e-20 88.2
MsaG009303 AT2G46770.1 40.367 109 34 2 28 109 76 180 2.20e-20 89.0
MsaG009303 AT3G17730.1 38.739 111 50 2 14 110 51 157 2.57e-20 86.7
MsaG009303 AT4G36160.1 42.708 96 39 2 26 109 68 159 4.00e-20 88.2
MsaG009303 AT4G36160.3 42.708 96 39 2 26 109 68 159 4.00e-20 88.2
MsaG009303 AT4G36160.2 42.708 96 39 2 26 109 72 163 4.09e-20 88.2
MsaG009303 AT1G33280.1 44.898 98 39 2 23 109 63 156 6.11e-20 86.7
MsaG009303 AT3G61910.1 39.091 110 36 2 28 110 71 176 7.75e-20 87.0
MsaG009303 AT2G18060.3 40.404 99 43 2 26 112 67 161 9.60e-20 87.0
MsaG009303 AT2G18060.1 40.404 99 43 2 26 112 67 161 9.60e-20 87.0
MsaG009303 AT1G65910.1 40.909 110 47 2 20 115 57 162 9.81e-20 88.2
MsaG009303 AT1G34190.1 37.097 124 60 2 1 110 48 167 1.70e-19 87.4
MsaG009303 AT5G53950.1 40.952 105 44 2 23 111 69 171 3.84e-19 85.5
MsaG009303 AT4G10350.2 39.655 116 49 4 13 111 5 116 5.50e-19 84.0
MsaG009303 AT3G15170.1 38.583 127 58 4 6 115 56 179 6.30e-19 84.0
MsaG009303 AT4G28530.1 45.263 95 40 2 25 109 80 172 1.03e-18 84.0
MsaG009303 AT3G03200.1 39.640 111 48 2 20 115 57 163 1.66e-18 84.3
MsaG009303 AT4G35580.3 43.000 100 39 2 24 109 64 159 1.73e-18 84.3
MsaG009303 AT4G35580.2 43.000 100 39 2 24 109 64 159 1.80e-18 84.3
MsaG009303 AT4G10350.1 41.346 104 42 3 23 111 64 163 1.91e-18 83.2
MsaG009303 AT1G54330.1 39.496 119 53 3 14 121 51 161 1.93e-18 82.8
MsaG009303 AT4G35580.1 43.000 100 39 2 24 109 64 159 2.01e-18 84.0
MsaG009303 AT5G17260.1 38.739 111 49 2 20 115 57 163 2.37e-18 83.6
MsaG009303 AT3G10500.1 37.069 116 55 2 14 115 54 165 3.17e-18 83.6
MsaG009303 AT1G34180.1 41.000 100 55 1 1 100 48 143 4.93e-18 82.8
MsaG009303 AT1G34180.3 41.000 100 55 1 1 100 48 143 5.73e-18 82.4
MsaG009303 AT5G04410.1 33.871 124 64 2 1 110 41 160 3.30e-17 80.5
MsaG009303 AT1G34180.4 48.718 78 36 1 23 100 82 155 4.39e-17 80.1
MsaG009303 AT1G32770.1 34.058 138 55 4 6 114 52 182 4.47e-17 79.3
MsaG009303 AT1G33060.1 40.000 100 42 2 24 109 79 174 4.65e-17 80.1
MsaG009303 AT1G33060.2 40.000 100 42 2 24 109 79 174 4.75e-17 80.1
MsaG009303 AT1G34180.2 48.718 78 36 1 23 100 82 155 5.14e-17 80.1
MsaG009303 AT1G32510.1 32.836 134 72 2 1 116 38 171 1.86e-16 77.0
MsaG009303 AT5G46590.1 33.884 121 62 2 5 110 42 159 4.06e-16 76.3
MsaG009303 AT5G22290.1 29.054 148 90 3 15 151 66 209 5.00e-16 76.3
MsaG009303 AT5G46590.2 38.542 96 56 1 5 100 42 134 5.47e-16 75.5
MsaG009303 AT3G10480.2 37.624 101 45 2 24 110 82 178 8.87e-16 76.3
MsaG009303 AT3G10480.1 37.255 102 45 2 24 110 82 179 1.17e-15 75.9
MsaG009303 AT5G22380.1 27.528 178 102 4 5 158 43 217 1.74e-15 73.6
MsaG009303 AT5G09330.5 32.432 111 57 2 14 110 51 157 2.94e-15 74.7
MsaG009303 AT5G09330.2 32.432 111 57 2 14 110 51 157 2.94e-15 74.7
MsaG009303 AT5G09330.4 32.432 111 57 2 14 110 51 157 2.94e-15 74.7
MsaG009303 AT5G09330.3 32.432 111 57 2 14 110 51 157 2.94e-15 74.7
MsaG009303 AT5G09330.1 32.432 111 57 2 14 110 51 157 2.94e-15 74.7
MsaG009303 AT4G01540.4 31.496 127 76 3 18 134 55 180 4.11e-15 73.9
MsaG009303 AT3G10480.3 37.255 102 45 2 24 110 115 212 4.26e-15 74.3
MsaG009303 AT3G44350.2 31.188 202 102 10 18 196 52 239 6.24e-15 72.0
MsaG009303 AT4G01540.3 31.496 127 76 3 18 134 55 180 6.25e-15 73.6
MsaG009303 AT4G01540.2 41.860 86 49 1 18 103 55 139 6.40e-15 73.6
MsaG009303 AT4G01540.1 41.860 86 49 1 18 103 55 139 7.15e-15 73.6
MsaG009303 AT5G24590.2 37.037 108 51 2 16 109 60 164 7.39e-15 73.6
MsaG009303 AT3G10490.2 36.275 102 46 2 24 110 82 179 1.03e-14 73.2
MsaG009303 AT4G01520.1 40.698 86 50 1 18 103 55 139 1.21e-14 72.0
MsaG009303 AT4G17980.1 37.624 101 45 2 26 110 63 161 1.27e-14 71.6
MsaG009303 AT2G27300.1 39.785 93 45 2 23 108 67 155 1.50e-14 72.0
MsaG009303 AT3G10490.1 36.275 102 46 2 24 110 82 179 1.60e-14 70.9
MsaG009303 AT4G17980.2 37.624 101 45 2 26 110 63 161 2.08e-14 71.2
MsaG009303 AT3G44290.2 27.222 180 109 6 15 182 59 228 2.37e-14 70.1
MsaG009303 AT3G44290.1 33.981 103 56 3 15 110 59 156 3.73e-14 70.9
MsaG009303 AT3G49530.2 34.259 108 54 3 16 109 60 164 7.48e-14 70.5
MsaG009303 AT3G44350.1 42.045 88 41 3 18 99 52 135 7.86e-14 67.4
MsaG009303 AT3G49530.1 34.259 108 54 3 16 109 60 164 1.31e-13 70.1
MsaG009303 AT2G18060.2 39.474 76 30 2 49 112 2 73 1.79e-13 68.6
MsaG009303 AT4G01550.1 37.234 94 58 1 10 103 46 138 1.46e-12 66.6
MsaG009303 AT1G32870.2 37.000 100 57 2 1 100 42 135 1.76e-12 66.6
MsaG009303 AT1G32870.1 37.000 100 57 2 1 100 42 135 1.79e-12 66.6
MsaG009303 AT1G32870.3 37.000 100 57 2 1 100 78 171 1.83e-12 66.6
MsaG009303 AT5G64060.1 29.730 111 60 2 14 110 51 157 4.65e-12 65.1
MsaG009303 AT5G04395.1 40.260 77 45 1 24 100 94 169 7.46e-12 62.8

Find 45 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATGGCATGCAGGAAATT+TGG 0.174862 2:+56993101 MsaT009303.1:intron
TCTCCTCCATTTGAGTATAT+TGG 0.237105 2:-56992440 None:intergenic
AGTTGTTATCATTTGATAAC+TGG 0.242113 2:+56993455 MsaT009303.1:CDS
CTCCATATTGTTGCCCATTC+TGG 0.244126 2:-56993224 None:intergenic
GAGTCACTGGCTCTGGATTC+TGG 0.244802 2:+56992396 MsaT009303.1:CDS
TAACACCATCATCAAATCTT+TGG 0.273173 2:+56993413 MsaT009303.1:CDS
CCAGAGCCAGTGACTCTATT+TGG 0.333205 2:-56992389 None:intergenic
CAACAAACATATATAAATTT+CGG 0.353707 2:+56993259 MsaT009303.1:CDS
TTAGCTAATTGTTTCATCTC+TGG 0.354898 2:-56993172 None:intergenic
ATGCATCATCCAATCAGTTT+TGG 0.361511 2:-56992528 None:intergenic
CACCAGAATGGGCAACAATA+TGG 0.367590 2:+56993222 MsaT009303.1:CDS
CGTTAATGTGATGATGTTCA+TGG 0.410482 2:-56993288 None:intergenic
CCAAATAGAGTCACTGGCTC+TGG 0.412912 2:+56992389 MsaT009303.1:CDS
ACCAAAGAGGAAGAAAATAT+AGG 0.417816 2:+56992354 MsaT009303.1:CDS
CGTAACACGGGCACCAGAAT+GGG 0.442225 2:+56993211 MsaT009303.1:CDS
GTTTATTACCGTGGTAGTGC+TGG 0.452034 2:+56992497 MsaT009303.1:CDS
TCTGGATTCTGGAAAGCAAC+TGG 0.467422 2:+56992407 MsaT009303.1:CDS
GATAAACCAATATACTCAAA+TGG 0.472311 2:+56992434 MsaT009303.1:CDS
TCGTAACACGGGCACCAGAA+TGG 0.473256 2:+56993210 MsaT009303.1:CDS
ATCAAATCTTTGGATTAAGC+AGG 0.475836 2:+56993423 MsaT009303.1:CDS
TTTGATAACTGGGATGAGCT+TGG 0.483520 2:+56993466 MsaT009303.1:CDS
GATAATGAATATGTTGTAGA+TGG 0.489983 2:+56991405 MsaT009303.1:CDS
GAAAAGGTACCAAAACTGAT+TGG 0.493324 2:+56992519 MsaT009303.1:CDS
AATTATATCAATTACAAACT+AGG 0.502539 2:-56993517 None:intergenic
TTGCTTGTATAATTATTGAC+TGG 0.508672 2:-56993319 None:intergenic
AAATGTAGCTGGAGCAGAGA+AGG 0.513323 2:+56992316 MsaT009303.1:CDS
CAAGCAATGATCAAAGGAAT+CGG 0.513538 2:+56993335 MsaT009303.1:CDS
TACCGTGGTAGTGCTGGAAA+AGG 0.513961 2:+56992503 MsaT009303.1:CDS
TATGCAGAAGCAAATGTAGC+TGG 0.529620 2:+56992305 MsaT009303.1:intron
ATATACTCAAATGGAGGAGA+AGG 0.541852 2:+56992443 MsaT009303.1:CDS
ACGTCAAGCCGTTCGTAACA+CGG 0.550583 2:+56993198 MsaT009303.1:CDS
GTTAGACCAAATAGAGTCAC+TGG 0.561770 2:+56992383 MsaT009303.1:CDS
TCTGGTGCCCGTGTTACGAA+CGG 0.565365 2:-56993206 None:intergenic
CGTCAAGCCGTTCGTAACAC+GGG 0.566912 2:+56993199 MsaT009303.1:CDS
ATTATACAAGCAATGATCAA+AGG 0.581251 2:+56993329 MsaT009303.1:CDS
GGAGAAGGAAATGATTGTGT+TGG 0.583944 2:+56992458 MsaT009303.1:CDS
GTTGTTATCATTTGATAACT+GGG 0.587359 2:+56993456 MsaT009303.1:CDS
TTAATCCAAAGATTTGATGA+TGG 0.593399 2:-56993418 None:intergenic
AAAACACTTGTTTATTACCG+TGG 0.603507 2:+56992488 MsaT009303.1:CDS
ATGAGCTTGGATCAGTTGTG+AGG 0.626748 2:+56993479 MsaT009303.1:CDS
TCAAAAGAAATGTATCACAA+AGG 0.638749 2:+56993140 MsaT009303.1:CDS
AAACCAATATACTCAAATGG+AGG 0.641619 2:+56992437 MsaT009303.1:CDS
AGATCAGTTGAGCTCTAGAG+TGG 0.646085 2:+56993366 MsaT009303.1:CDS
TATGAACGATGATTTCAACA+CGG 0.741102 2:+56991383 MsaT009303.1:CDS
TGAACATCATCACATTAACG+AGG 0.762973 2:+56993291 MsaT009303.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 56991378 56993528 56991378 ID=MsaG009303
Chr2 mRNA 56991378 56993528 56991378 ID=MsaT009303.1;Parent=MsaG009303
Chr2 exon 56991378 56991426 56991378 ID=MsaT009303.1.exon1;Parent=MsaT009303.1
Chr2 CDS 56991378 56991426 56991378 ID=cds.MsaT009303.1;Parent=MsaT009303.1
Chr2 exon 56992312 56992556 56992312 ID=MsaT009303.1.exon2;Parent=MsaT009303.1
Chr2 CDS 56992312 56992556 56992312 ID=cds.MsaT009303.1;Parent=MsaT009303.1
Chr2 exon 56993115 56993528 56993115 ID=MsaT009303.1.exon3;Parent=MsaT009303.1
Chr2 CDS 56993115 56993528 56993115 ID=cds.MsaT009303.1;Parent=MsaT009303.1
Gene Sequence

>MsaT009303.1

ATGGGTATGAACGATGATTTCAACACGGATAATGAATATGTTGTAGATGAAGCAAATGTAGCTGGAGCAGAGAAGGAATGTTACTTTTTTTACCAAAGAGGAAGAAAATATAGGAACAGCGTTAGACCAAATAGAGTCACTGGCTCTGGATTCTGGAAAGCAACTGGAATAGATAAACCAATATACTCAAATGGAGGAGAAGGAAATGATTGTGTTGGACTCAAAAAAACACTTGTTTATTACCGTGGTAGTGCTGGAAAAGGTACCAAAACTGATTGGATGATGCATGAGTTTGAAATTTGGACATTGTGTAGAATTTTCAAAAGAAATGTATCACAAAGGAAACAAATACCAGAGATGAAACAATTAGCTAACAAACGTCAAGCCGTTCGTAACACGGGCACCAGAATGGGCAACAATATGGAGTTTAACACAAATCAACAAACATATATAAATTTCGGTGCAAGCCATGAACATCATCACATTAACGAGGACAAACCAGTCAATAATTATACAAGCAATGATCAAAGGAATCGGTTTCATGTAGATCAGTTGAGCTCTAGAGTGGCTCATCAACATCAACAAAATAATGTAACACCATCATCAAATCTTTGGATTAAGCAGGATGCTAATGAGTTGTTATCATTTGATAACTGGGATGAGCTTGGATCAGTTGTGAGGTTTGCTGTTGATTCACCTAGTTTGTAA
Protein sequence

>MsaT009303.1

MGMNDDFNTDNEYVVDEANVAGAEKECYFFYQRGRKYRNSVRPNRVTGSGFWKATGIDKPIYSNGGEGNDCVGLKKTLVYYRGSAGKGTKTDWMMHEFEIWTLCRIFKRNVSQRKQIPEMKQLANKRQAVRNTGTRMGNNMEFNTNQQTYINFGASHEHHHINEDKPVNNYTSNDQRNRFHVDQLSSRVAHQHQQNNVTPSSNLWIKQDANELLSFDNWDELGSVVRFAVDSPSL*