AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG009355

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009355 MtrunA17_Chr2g0312321 98.810 252 2 1 7 258 56 306 0.0 520
MsaG009355 MtrunA17_Chr6g0484441 44.211 190 89 4 9 186 54 238 3.78e-47 159
MsaG009355 MtrunA17_Chr1g0183741 44.560 193 90 4 7 197 57 234 2.71e-45 153
MsaG009355 MtrunA17_Chr7g0253011 55.333 150 54 5 7 143 64 213 1.97e-44 154
MsaG009355 MtrunA17_Chr4g0060931 51.266 158 67 4 9 159 66 220 2.51e-44 153
MsaG009355 MtrunA17_Chr6g0453451 44.624 186 90 3 9 193 69 242 6.34e-44 150
MsaG009355 MtrunA17_Chr7g0261411 67.308 104 27 2 7 108 57 155 9.73e-44 149
MsaG009355 MtrunA17_Chr6g0461661 55.970 134 49 4 14 137 72 205 1.09e-40 144
MsaG009355 MtrunA17_Chr3g0091921 53.285 137 55 3 9 138 65 199 1.15e-40 142
MsaG009355 MtrunA17_Chr2g0316971 63.551 107 36 2 7 110 70 176 4.54e-39 139
MsaG009355 MtrunA17_Chr1g0200561 53.509 114 44 3 1 109 59 168 3.27e-38 137
MsaG009355 MtrunA17_Chr3g0113001 44.509 173 70 5 9 168 14 173 6.75e-38 134
MsaG009355 MtrunA17_Chr3g0139331 60.194 103 36 2 9 109 60 159 1.00e-37 135
MsaG009355 MtrunA17_Chr1g0147421 57.522 113 43 2 9 119 77 186 1.31e-37 135
MsaG009355 MtrunA17_Chr4g0018391 53.509 114 44 3 1 109 47 156 1.36e-37 135
MsaG009355 MtrunA17_Chr5g0405211 58.333 96 35 2 15 109 66 157 3.19e-36 131
MsaG009355 MtrunA17_Chr8g0372121 52.941 102 45 1 15 116 64 162 7.83e-36 129
MsaG009355 MtrunA17_Chr2g0309891 57.843 102 43 0 15 116 63 164 7.84e-36 130
MsaG009355 MtrunA17_Chr4g0056541 50.833 120 52 3 1 116 47 163 1.45e-35 130
MsaG009355 MtrunA17_Chr8g0359921 44.828 145 74 2 9 147 63 207 6.89e-35 127
MsaG009355 MtrunA17_Chr5g0398971 40.331 181 97 6 15 188 65 241 1.73e-34 127
MsaG009355 MtrunA17_Chr4g0017921 51.327 113 47 2 1 109 48 156 1.88e-34 127
MsaG009355 MtrunA17_Chr7g0217721 39.444 180 89 5 7 168 66 243 2.91e-33 124
MsaG009355 MtrunA17_Chr7g0252971 47.059 119 51 2 9 115 57 175 8.18e-33 122
MsaG009355 MtrunA17_Chr8g0346001 48.624 109 48 2 15 115 73 181 1.74e-32 123
MsaG009355 MtrunA17_Chr5g0417791 50.943 106 49 2 7 109 50 155 2.92e-32 119
MsaG009355 MtrunA17_Chr8g0384721 39.227 181 89 6 7 176 54 224 3.24e-32 119
MsaG009355 MtrunA17_Chr2g0318081 52.679 112 45 3 9 117 65 171 3.48e-32 120
MsaG009355 MtrunA17_Chr7g0217711 62.963 81 30 0 7 87 66 146 4.46e-32 116
MsaG009355 MtrunA17_Chr2g0309331 55.670 97 37 3 15 109 68 160 6.30e-32 120
MsaG009355 MtrunA17_Chr3g0144271 60.227 88 33 1 7 92 71 158 7.58e-32 121
MsaG009355 MtrunA17_Chr6g0481091 48.624 109 55 1 15 123 63 170 2.10e-31 116
MsaG009355 MtrunA17_Chr4g0042561 54.082 98 42 2 15 109 64 161 2.18e-31 117
MsaG009355 MtrunA17_Chr3g0109821 51.818 110 50 1 15 124 63 169 3.09e-31 121
MsaG009355 MtrunA17_Chr3g0113031 44.304 158 65 4 23 168 3 149 4.19e-31 116
MsaG009355 MtrunA17_Chr6g0453131 48.718 117 51 3 7 114 62 178 1.79e-30 117
MsaG009355 MtrunA17_Chr7g0263931 53.271 107 37 3 9 106 60 162 2.85e-30 115
MsaG009355 MtrunA17_Chr7g0214071 45.161 124 54 3 14 126 66 186 3.18e-30 113
MsaG009355 MtrunA17_Chr4g0047841 42.958 142 57 3 9 126 64 205 5.94e-30 114
MsaG009355 MtrunA17_Chr3g0124761 35.965 228 107 8 9 203 56 277 1.07e-29 112
MsaG009355 MtrunA17_Chr5g0433781 47.107 121 60 2 15 135 63 179 1.69e-29 116
MsaG009355 MtrunA17_Chr5g0428991 52.427 103 38 2 15 112 82 178 2.36e-29 116
MsaG009355 MtrunA17_Chr2g0318101 48.673 113 46 3 9 109 64 176 2.42e-29 113
MsaG009355 MtrunA17_Chr3g0128151 53.398 103 37 2 15 112 79 175 3.98e-29 115
MsaG009355 MtrunA17_Chr8g0361731 46.610 118 53 3 9 116 63 180 1.34e-28 112
MsaG009355 MtrunA17_Chr2g0326361 49.524 105 48 2 7 106 61 165 2.02e-28 110
MsaG009355 MtrunA17_Chr5g0417001 45.299 117 55 2 9 116 63 179 2.21e-28 111
MsaG009355 MtrunA17_Chr3g0130101 51.961 102 40 2 12 109 61 157 2.90e-28 110
MsaG009355 MtrunA17_Chr3g0128111 40.690 145 74 4 15 154 99 236 4.76e-28 107
MsaG009355 MtrunA17_Chr2g0311661 34.673 199 103 5 14 185 76 274 6.21e-28 108
MsaG009355 MtrunA17_Chr4g0038751 49.020 102 43 2 11 109 66 161 1.73e-27 107
MsaG009355 MtrunA17_Chr3g0130731 50.980 102 47 2 14 113 61 161 1.74e-27 107
MsaG009355 MtrunA17_Chr8g0364791 48.980 98 49 1 12 109 60 156 1.84e-27 109
MsaG009355 MtrunA17_Chr3g0092671 51.042 96 45 1 15 110 63 156 5.94e-27 108
MsaG009355 MtrunA17_Chr4g0076731 36.816 201 106 6 9 202 103 289 8.67e-27 107
MsaG009355 MtrunA17_Chr1g0197351 49.074 108 38 2 14 110 98 199 8.82e-27 108
MsaG009355 MtrunA17_Chr5g0400461 50.000 106 42 2 15 113 63 164 1.09e-26 105
MsaG009355 MtrunA17_Chr1g0197361 51.042 96 45 1 15 110 66 159 1.40e-26 108
MsaG009355 MtrunA17_Chr8g0384201 45.918 98 52 1 12 109 60 156 5.23e-26 105
MsaG009355 MtrunA17_Chr2g0311651 36.170 188 83 6 14 176 77 252 5.63e-26 103
MsaG009355 MtrunA17_Chr5g0442771 37.725 167 72 5 7 146 65 226 3.42e-25 101
MsaG009355 MtrunA17_Chr1g0201021 56.000 75 33 0 12 86 73 147 3.55e-25 103
MsaG009355 MtrunA17_Chr4g0054791 50.459 109 43 4 15 115 63 168 1.22e-24 100
MsaG009355 MtrunA17_Chr7g0243371 46.512 86 44 1 14 97 18 103 1.05e-22 90.1
MsaG009355 MtrunA17_Chr2g0321741 44.565 92 43 1 15 106 48 131 2.69e-22 94.4
MsaG009355 MtrunA17_Chr3g0131911 39.450 109 57 2 12 111 64 172 2.28e-20 87.0
MsaG009355 MtrunA17_Chr8g0345671 46.809 94 41 3 15 106 69 155 8.72e-20 87.8
MsaG009355 MtrunA17_Chr8g0385991 36.538 104 60 2 14 111 66 169 5.89e-19 82.8
MsaG009355 MtrunA17_Chr2g0321631 40.367 109 55 4 6 107 55 160 6.76e-19 83.2
MsaG009355 MtrunA17_Chr8g0345691 43.617 94 44 3 15 106 70 156 2.38e-18 83.6
MsaG009355 MtrunA17_Chr7g0266701 32.787 122 74 3 5 118 55 176 7.79e-17 77.8
MsaG009355 MtrunA17_Chr4g0026141 37.000 100 54 3 14 107 64 160 2.43e-16 76.3
MsaG009355 MtrunA17_Chr8g0345741 43.836 73 38 2 14 84 48 119 2.23e-14 68.9
MsaG009355 MtrunA17_Chr8g0345721 44.444 72 37 2 14 83 121 191 2.52e-14 70.1
MsaG009355 MtrunA17_Chr4g0050701 31.746 126 71 3 9 127 60 177 4.61e-13 66.2
MsaG009355 MtrunA17_Chr8g0384351 36.000 125 56 7 2 126 53 153 7.40e-12 62.8
MsaG009355 MtrunA17_Chr8g0345701 43.678 87 30 4 14 84 64 147 8.23e-12 62.4
MsaG009355 MtrunA17_Chr8g0345711 43.056 72 38 2 15 84 70 140 1.14e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009355 AT1G56010.1 58.712 264 84 9 9 256 2 256 9.97e-96 281
MsaG009355 AT1G56010.2 58.271 266 86 9 7 256 67 323 1.23e-94 281
MsaG009355 AT3G12977.2 51.373 255 80 10 6 256 11 225 8.13e-73 222
MsaG009355 AT3G12977.1 50.794 252 84 8 7 256 65 278 1.61e-70 218
MsaG009355 AT5G61430.2 50.000 190 75 6 9 183 2 186 8.89e-50 165
MsaG009355 AT5G61430.1 50.000 190 75 6 9 183 65 249 7.84e-49 164
MsaG009355 AT5G07680.2 42.857 252 116 7 9 249 52 286 3.17e-46 157
MsaG009355 AT5G07680.1 42.857 252 116 7 9 249 66 300 4.19e-46 157
MsaG009355 AT5G39610.1 51.634 153 68 3 9 159 69 217 6.62e-44 150
MsaG009355 AT3G18400.1 66.337 101 32 1 9 109 54 152 7.64e-44 150
MsaG009355 AT3G04060.1 44.500 200 97 5 7 194 67 264 1.49e-43 150
MsaG009355 AT4G28530.1 65.741 108 30 2 7 112 73 175 4.44e-43 150
MsaG009355 AT5G18270.2 62.500 112 40 2 9 118 70 181 5.21e-43 149
MsaG009355 AT5G18270.1 62.500 112 40 2 9 118 70 181 5.61e-43 149
MsaG009355 AT1G76420.1 62.857 105 35 2 7 109 69 171 4.16e-40 141
MsaG009355 AT3G29035.1 46.667 180 76 7 9 171 73 249 9.33e-40 140
MsaG009355 AT3G15170.1 46.023 176 72 4 7 171 67 230 3.51e-39 138
MsaG009355 AT1G71930.2 53.097 113 46 2 1 109 49 158 4.14e-39 139
MsaG009355 AT1G71930.1 53.097 113 46 2 1 109 49 158 4.14e-39 139
MsaG009355 AT5G53950.1 63.636 110 37 2 9 115 66 175 5.29e-39 139
MsaG009355 AT2G24430.2 62.264 106 34 3 7 109 63 165 1.42e-38 137
MsaG009355 AT2G24430.1 62.264 106 34 3 7 109 63 165 1.42e-38 137
MsaG009355 AT5G62380.2 60.825 97 33 2 14 109 64 156 1.95e-37 135
MsaG009355 AT5G62380.1 60.825 97 33 2 14 109 64 156 1.95e-37 135
MsaG009355 AT1G12260.2 35.938 256 117 10 1 221 47 290 2.47e-37 134
MsaG009355 AT2G18060.3 47.407 135 63 3 15 144 67 198 5.17e-37 134
MsaG009355 AT2G18060.1 47.407 135 63 3 15 144 67 198 5.17e-37 134
MsaG009355 AT1G62700.1 50.388 129 53 4 15 142 65 183 1.90e-36 133
MsaG009355 AT1G62700.2 50.388 129 53 4 15 142 65 183 1.90e-36 133
MsaG009355 AT1G12260.1 47.101 138 60 4 1 129 47 180 3.11e-36 132
MsaG009355 AT4G36160.2 51.327 113 48 2 1 109 54 163 3.78e-36 132
MsaG009355 AT4G36160.1 51.327 113 48 2 1 109 50 159 3.79e-36 132
MsaG009355 AT4G36160.3 51.327 113 48 2 1 109 50 159 3.79e-36 132
MsaG009355 AT4G10350.2 52.212 113 49 2 1 109 2 113 6.15e-36 129
MsaG009355 AT4G10350.1 57.895 95 39 1 15 109 67 160 5.72e-35 128
MsaG009355 AT1G33280.1 58.947 95 35 1 15 109 66 156 2.30e-34 125
MsaG009355 AT3G61910.1 46.825 126 51 3 1 110 51 176 8.72e-34 125
MsaG009355 AT5G66300.1 52.885 104 43 2 15 116 70 169 1.30e-33 123
MsaG009355 AT2G46770.1 44.800 125 53 2 1 109 56 180 7.12e-33 123
MsaG009355 AT4G27410.2 55.446 101 44 1 9 109 63 162 1.70e-32 120
MsaG009355 AT3G04070.2 47.500 120 52 2 9 117 59 178 2.89e-32 121
MsaG009355 AT4G27410.3 55.446 101 44 1 9 109 80 179 3.00e-32 120
MsaG009355 AT1G79580.3 55.208 96 38 2 15 109 75 166 5.41e-32 121
MsaG009355 AT1G79580.5 55.208 96 38 2 15 109 75 166 5.41e-32 121
MsaG009355 AT1G79580.4 55.208 96 38 2 15 109 75 166 5.41e-32 121
MsaG009355 AT1G79580.2 55.208 96 38 2 15 109 75 166 5.41e-32 121
MsaG009355 AT1G79580.1 55.208 96 38 2 15 109 75 166 5.41e-32 121
MsaG009355 AT1G52890.1 53.153 111 44 3 9 116 63 168 8.16e-32 119
MsaG009355 AT4G35580.1 58.763 97 35 2 15 109 66 159 9.27e-32 122
MsaG009355 AT3G15500.1 53.846 104 41 2 9 109 63 162 9.34e-32 119
MsaG009355 AT4G35580.3 58.763 97 35 2 15 109 66 159 1.03e-31 122
MsaG009355 AT3G17730.1 55.208 96 42 1 15 110 63 157 1.05e-31 117
MsaG009355 AT4G35580.2 58.763 97 35 2 15 109 66 159 1.06e-31 122
MsaG009355 AT3G10490.1 40.506 158 75 2 14 171 83 221 1.11e-31 117
MsaG009355 AT3G10480.1 47.287 129 58 2 14 135 83 208 1.93e-31 120
MsaG009355 AT3G10480.3 47.287 129 58 2 14 135 116 241 4.36e-31 120
MsaG009355 AT3G10490.2 55.670 97 43 0 14 110 83 179 8.04e-31 119
MsaG009355 AT1G32770.1 31.148 244 141 9 1 222 56 294 1.06e-30 117
MsaG009355 AT1G69490.1 49.541 109 46 2 7 109 56 161 1.57e-30 115
MsaG009355 AT1G54330.1 57.843 102 39 2 15 116 63 160 2.42e-30 115
MsaG009355 AT3G10480.2 47.287 129 57 3 14 135 83 207 2.73e-30 117
MsaG009355 AT1G65910.1 55.789 95 41 1 15 109 63 156 2.98e-30 119
MsaG009355 AT1G33060.2 36.041 197 92 6 2 176 68 252 5.15e-30 118
MsaG009355 AT1G01720.1 36.538 208 101 7 9 194 56 254 5.48e-30 114
MsaG009355 AT1G33060.1 36.041 197 92 6 2 176 68 252 5.51e-30 118
MsaG009355 AT3G03200.1 48.649 111 56 1 14 124 62 171 6.02e-30 117
MsaG009355 AT3G04070.1 41.912 136 52 2 9 117 59 194 7.45e-30 115
MsaG009355 AT5G08790.1 49.533 107 49 2 7 109 54 159 1.05e-29 113
MsaG009355 AT5G63790.2 50.467 107 48 2 7 109 107 212 1.14e-29 114
MsaG009355 AT5G63790.1 50.467 107 48 2 7 109 97 202 1.20e-29 113
MsaG009355 AT1G61110.1 45.161 124 53 4 9 120 65 185 1.33e-29 114
MsaG009355 AT5G17260.1 52.083 96 46 0 14 109 62 157 1.95e-29 115
MsaG009355 AT5G46590.1 52.941 102 46 2 15 115 63 163 2.27e-29 112
MsaG009355 AT3G10500.1 52.083 96 45 1 15 110 66 160 7.09e-29 114
MsaG009355 AT5G04410.1 52.083 96 45 1 15 110 66 160 8.84e-29 114
MsaG009355 AT1G32510.1 50.459 109 47 1 15 116 63 171 9.58e-29 110
MsaG009355 AT3G15510.1 48.246 114 47 3 9 110 66 179 1.35e-28 112
MsaG009355 AT2G33480.3 50.515 97 47 1 15 111 10 105 2.17e-28 107
MsaG009355 AT4G17980.1 46.825 126 57 3 15 130 63 188 4.40e-28 108
MsaG009355 AT4G17980.2 46.825 126 57 3 15 130 63 188 4.82e-28 108
MsaG009355 AT1G77450.1 49.524 105 50 2 7 109 57 160 6.27e-28 107
MsaG009355 AT2G02450.2 53.000 100 43 3 9 107 100 196 9.83e-28 110
MsaG009355 AT2G02450.1 51.961 102 45 3 9 109 100 198 1.15e-27 109
MsaG009355 AT2G43000.1 40.299 134 77 1 8 138 66 199 1.57e-27 107
MsaG009355 AT2G33480.2 51.546 97 44 1 15 111 67 160 1.77e-27 107
MsaG009355 AT2G33480.1 50.515 97 47 1 15 111 67 162 1.96e-27 107
MsaG009355 AT5G39820.1 42.308 130 53 4 9 116 69 198 8.58e-27 106
MsaG009355 AT5G46590.2 61.111 72 27 1 15 85 63 134 8.88e-27 105
MsaG009355 AT5G13180.1 48.077 104 42 3 14 111 65 162 1.23e-26 104
MsaG009355 AT1G26870.1 38.926 149 52 4 9 141 65 190 3.03e-26 106
MsaG009355 AT1G52880.1 45.378 119 54 2 9 116 66 184 3.13e-26 104
MsaG009355 AT2G17040.1 48.039 102 44 2 11 109 57 152 5.76e-26 103
MsaG009355 AT1G34180.3 44.444 108 59 1 2 109 60 166 5.87e-26 105
MsaG009355 AT5G24590.2 45.238 126 64 4 15 137 70 193 1.10e-25 105
MsaG009355 AT1G34180.4 46.465 99 52 1 11 109 81 178 1.44e-25 104
MsaG009355 AT1G34180.1 44.037 109 60 1 2 110 60 167 1.51e-25 105
MsaG009355 AT1G34180.2 46.465 99 52 1 11 109 81 178 3.51e-25 104
MsaG009355 AT1G34190.1 50.588 85 42 0 2 86 60 144 9.55e-25 103
MsaG009355 AT2G18060.2 43.750 112 55 3 38 144 2 110 1.05e-24 99.8
MsaG009355 AT5G09330.5 42.424 99 56 1 12 110 60 157 2.53e-24 101
MsaG009355 AT5G09330.2 42.424 99 56 1 12 110 60 157 2.53e-24 101
MsaG009355 AT5G09330.4 42.424 99 56 1 12 110 60 157 2.53e-24 101
MsaG009355 AT5G09330.3 42.424 99 56 1 12 110 60 157 2.53e-24 101
MsaG009355 AT5G09330.1 42.424 99 56 1 12 110 60 157 2.53e-24 101
MsaG009355 AT3G12910.1 46.296 108 44 4 14 107 76 183 6.93e-23 95.5
MsaG009355 AT3G10490.4 56.410 78 34 0 33 110 2 79 1.22e-22 95.5
MsaG009355 AT3G10490.3 56.410 78 34 0 33 110 2 79 1.22e-22 95.5
MsaG009355 AT2G27300.1 43.478 115 53 4 15 126 70 175 1.58e-22 95.1
MsaG009355 AT3G49530.2 39.831 118 69 2 15 131 70 186 3.29e-22 95.1
MsaG009355 AT3G49530.1 44.000 100 54 2 15 113 70 168 4.02e-22 95.1
MsaG009355 AT5G22290.1 43.878 98 47 2 15 112 77 166 6.98e-22 93.2
MsaG009355 AT2G43000.2 39.167 120 70 1 22 138 2 121 9.56e-22 90.1
MsaG009355 AT3G44290.2 44.565 92 43 1 15 106 70 153 4.87e-21 89.0
MsaG009355 AT5G64060.1 37.736 106 63 2 7 110 53 157 5.11e-21 91.3
MsaG009355 AT1G32870.2 44.144 111 57 2 14 124 66 171 6.22e-21 92.0
MsaG009355 AT1G32870.1 44.144 111 57 2 14 124 66 171 6.80e-21 92.0
MsaG009355 AT1G32870.3 44.144 111 57 2 14 124 102 207 9.79e-21 91.7
MsaG009355 AT3G44290.1 44.565 92 43 1 15 106 70 153 1.92e-20 89.4
MsaG009355 AT4G01540.3 39.130 115 56 5 15 122 63 170 2.25e-18 84.3
MsaG009355 AT4G01540.4 39.130 115 56 5 15 122 63 170 2.56e-18 84.0
MsaG009355 AT4G01540.2 39.130 115 56 5 15 122 63 170 3.21e-18 84.0
MsaG009355 AT4G01540.1 39.130 115 56 5 15 122 63 170 3.36e-18 84.0
MsaG009355 AT4G01520.1 38.261 115 57 5 15 122 63 170 3.55e-18 82.4
MsaG009355 AT4G01550.1 42.000 100 49 3 16 112 63 156 3.74e-18 83.6
MsaG009355 AT5G22380.1 40.000 105 60 3 6 107 56 160 1.16e-17 80.1
MsaG009355 AT3G44350.2 37.864 103 62 2 10 110 59 161 1.29e-17 80.1
MsaG009355 AT4G28530.2 41.509 106 22 2 7 112 73 138 1.80e-17 80.9
MsaG009355 AT5G04395.1 46.667 75 37 1 14 85 95 169 3.12e-16 75.1
MsaG009355 AT3G04420.2 33.333 135 80 3 16 148 63 189 1.41e-15 75.1
MsaG009355 AT3G04420.1 30.000 180 101 6 16 183 63 229 2.43e-15 75.1
MsaG009355 AT3G44350.1 45.455 77 40 2 10 84 59 135 2.60e-15 72.0
MsaG009355 AT5G22290.2 44.595 74 33 2 39 112 2 67 1.04e-14 72.0
MsaG009355 AT1G02230.1 28.977 176 112 4 16 188 63 228 1.13e-14 73.2

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCACTTCCAGCATTAA+TGG 0.146600 2:+57619490 MsaT009355.1:CDS
ATTAATGATCAAAACCTAAA+AGG 0.212913 2:+57619776 MsaT009355.1:CDS
TCCTAAAATTTCATCTTCTA+AGG 0.243571 2:+57618416 MsaT009355.1:CDS
GTTCCATATTGGTTGTTATA+TGG 0.295352 2:-57619616 None:intergenic
GTGCAACTGCATCAGGTTAT+TGG 0.304123 2:+57618244 MsaT009355.1:CDS
TTGTTGGGTAGAGGTAATTT+TGG 0.309334 2:-57619638 None:intergenic
CAACCATATAACAACCAATA+TGG 0.320797 2:+57619613 MsaT009355.1:CDS
GTAGCCCTGTTGCATATTTA+CGG 0.328714 2:-57618212 None:intergenic
AACAACAATGCAAGTACATT+TGG 0.349859 2:+57619662 MsaT009355.1:CDS
ACTTGCATTGTTGTTATTGT+TGG 0.362387 2:-57619654 None:intergenic
ATTAATGTAATGGAAATATA+TGG 0.369538 2:+57618135 None:intergenic
CTTCGTAAGGGCACTCTTGT+TGG 0.373702 2:+57618294 MsaT009355.1:CDS
GATAAAGGGTCTAAGCTATA+AGG 0.378533 2:-57619692 None:intergenic
GGTTGTAGATTGGGTTGGTT+TGG 0.395494 2:-57619595 None:intergenic
TTGTTATATGGTTGTAGATT+GGG 0.402170 2:-57619604 None:intergenic
GTTGTTATATGGTTGTAGAT+TGG 0.403136 2:-57619605 None:intergenic
AGAGACCGTAAATATGCAAC+AGG 0.409929 2:+57618207 MsaT009355.1:CDS
TTGTGTATATACAGGAAGAT+TGG 0.410383 2:+57619384 MsaT009355.1:intron
TGTGTATATACAGGAAGATT+GGG 0.427064 2:+57619385 MsaT009355.1:intron
TTTGAGTCAACTATCAAAGA+TGG 0.431620 2:+57619742 MsaT009355.1:CDS
TTGAGAAAACATGGAGAAGC+AGG 0.435570 2:-57619570 None:intergenic
TTAATGATCAAAACCTAAAA+GGG 0.436538 2:+57619777 MsaT009355.1:CDS
ACAAATCGTGCAACTGCATC+AGG 0.442887 2:+57618237 MsaT009355.1:CDS
CTTGCTCATATTCATCTGTA+TGG 0.443475 2:-57619544 None:intergenic
CTTGCATTGTTGTTATTGTT+GGG 0.444732 2:-57619653 None:intergenic
TCAGGTTATTGGAAGGCAAC+AGG 0.459668 2:+57618255 MsaT009355.1:CDS
ATCAAGGAAGGGCACCTAAA+GGG 0.466310 2:+57618340 MsaT009355.1:CDS
AAGACTTTAGTGTTCTATCA+AGG 0.477018 2:+57618324 MsaT009355.1:CDS
TGCTACAAAACCTCCAAGCA+TGG 0.497760 2:+57619442 MsaT009355.1:CDS
ATGCATGAGTTTCGTATTGA+GGG 0.498893 2:+57618381 MsaT009355.1:CDS
TGAGAGTTACTTATCTGAAG+TGG 0.505837 2:+57619829 MsaT009355.1:CDS
AATGCATGAGTTTCGTATTG+AGG 0.506210 2:+57618380 MsaT009355.1:CDS
CATCTGTATGGAATTGAGCT+TGG 0.508894 2:-57619532 None:intergenic
AAAGGGTCATCACCAAGCTT+AGG 0.523339 2:+57619794 MsaT009355.1:CDS
TTTCGTATTGAGGGTCCTCA+TGG 0.526305 2:+57618390 MsaT009355.1:CDS
CTTTAGTGTTCTATCAAGGA+AGG 0.528388 2:+57618328 MsaT009355.1:CDS
AACAATGCAAGTACATTTGG+AGG 0.528454 2:+57619665 MsaT009355.1:CDS
GGACAGAGCTATCCTTCGTA+AGG 0.530042 2:+57618281 MsaT009355.1:CDS
ATGTAAGAATCCATTAATGC+TGG 0.533005 2:-57619500 None:intergenic
TCATAGCAACTACCCATGCT+TGG 0.533828 2:-57619455 None:intergenic
TATCAAGGAAGGGCACCTAA+AGG 0.534747 2:+57618339 MsaT009355.1:CDS
GAACTTCCTTCACCTAAGCT+TGG 0.537127 2:-57619806 None:intergenic
TTGTTGTTATTGTTGGGTAG+AGG 0.544900 2:-57619647 None:intergenic
TAGCAACTACCCATGCTTGG+AGG 0.547765 2:-57619452 None:intergenic
GAGAGTTACTTATCTGAAGT+GGG 0.548125 2:+57619830 MsaT009355.1:CDS
TATATGGTTGTAGATTGGGT+TGG 0.552281 2:-57619600 None:intergenic
TTAGAAATTGTTCCACATGT+GGG 0.570954 2:-57619858 None:intergenic
TAGAAATTGTTCCACATGTG+GGG 0.571008 2:-57619857 None:intergenic
ATTAGAAATTGTTCCACATG+TGG 0.572291 2:-57619859 None:intergenic
GCTACAAAACCTCCAAGCAT+GGG 0.600781 2:+57619443 MsaT009355.1:CDS
CATGTGTGGGAGGGAAAGAG+TGG 0.608228 2:+57618169 MsaT009355.1:CDS
CAGAAGCAGCATGTGTGGGA+GGG 0.608897 2:+57618160 MsaT009355.1:CDS
TTTAGTGTTCTATCAAGGAA+GGG 0.615096 2:+57618329 MsaT009355.1:CDS
TTATTGGAAGGCAACAGGAA+AGG 0.621123 2:+57618260 MsaT009355.1:CDS
TCATCACCAAGCTTAGGTGA+AGG 0.632923 2:+57619800 MsaT009355.1:CDS
AACTGCATCAGGTTATTGGA+AGG 0.632925 2:+57618248 MsaT009355.1:CDS
GACAGAGCTATCCTTCGTAA+GGG 0.632999 2:+57618282 MsaT009355.1:CDS
TGAGAAAACATGGAGAAGCA+GGG 0.639023 2:-57619569 None:intergenic
TGGTGCAGAAGCAGCATGTG+TGG 0.642823 2:+57618155 MsaT009355.1:CDS
GCAGAAGCAGCATGTGTGGG+AGG 0.644425 2:+57618159 MsaT009355.1:CDS
CAACAAGAGTGCCCTTACGA+AGG 0.654867 2:-57618293 None:intergenic
GGTAGTTGCTATGATGACAC+AGG 0.669164 2:+57619464 MsaT009355.1:CDS
AAGTGGGTATGCCCCACATG+TGG 0.670092 2:+57619846 MsaT009355.1:CDS
GGTGCAGAAGCAGCATGTGT+GGG 0.686440 2:+57618156 MsaT009355.1:CDS
GAGACCGTAAATATGCAACA+GGG 0.752701 2:+57618208 MsaT009355.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 57618144 57619880 57618144 ID=MsaG009355
Chr2 mRNA 57618144 57619880 57618144 ID=MsaT009355.1;Parent=MsaG009355
Chr2 exon 57618144 57618437 57618144 ID=MsaT009355.1.exon1;Parent=MsaT009355.1
Chr2 CDS 57618144 57618437 57618144 ID=cds.MsaT009355.1;Parent=MsaT009355.1
Chr2 exon 57619398 57619880 57619398 ID=MsaT009355.1.exon2;Parent=MsaT009355.1
Chr2 CDS 57619398 57619880 57619398 ID=cds.MsaT009355.1;Parent=MsaT009355.1
Gene Sequence

>MsaT009355.1

ATGGAAATATATGGTGCAGAAGCAGCATGTGTGGGAGGGAAAGAGTGGTATTTCTATACACAAAGAGACCGTAAATATGCAACAGGGCTACGCACAAATCGTGCAACTGCATCAGGTTATTGGAAGGCAACAGGAAAGGACAGAGCTATCCTTCGTAAGGGCACTCTTGTTGGAATGAGAAAGACTTTAGTGTTCTATCAAGGAAGGGCACCTAAAGGGAGAAAAACTGAATGGGTAATGCATGAGTTTCGTATTGAGGGTCCTCATGGCCCTCCTAAAATTTCATCTTCTAAGGAAGATTGGGTTTTGTGTAGGGTGTTCTACAAAAACAGAGAAGTTGCTACAAAACCTCCAAGCATGGGTAGTTGCTATGATGACACAGGCTCTTCATCACTTCCAGCATTAATGGATTCTTACATAAGTTTTGACCAAGCTCAATTCCATACAGATGAATATGAGCAAGTGCCCTGCTTCTCCATGTTTTCTCAAAACCAAACCAACCCAATCTACAACCATATAACAACCAATATGGAACCAAAATTACCTCTACCCAACAATAACAACAATGCAAGTACATTTGGAGGAGCACCTTATAGCTTAGACCCTTTATCATGTGATAGAAAAGTGTTGAAAGCTGTTTTGAGTCAACTATCAAAGATGGAAAGAAACCCTATTAATGATCAAAACCTAAAAGGGTCATCACCAAGCTTAGGTGAAGGAAGTTCTGAGAGTTACTTATCTGAAGTGGGTATGCCCCACATGTGGAACAATTTCTAA
Protein sequence

>MsaT009355.1

MEIYGAEAACVGGKEWYFYTQRDRKYATGLRTNRATASGYWKATGKDRAILRKGTLVGMRKTLVFYQGRAPKGRKTEWVMHEFRIEGPHGPPKISSSKEDWVLCRVFYKNREVATKPPSMGSCYDDTGSSSLPALMDSYISFDQAQFHTDEYEQVPCFSMFSQNQTNPIYNHITTNMEPKLPLPNNNNNASTFGGAPYSLDPLSCDRKVLKAVLSQLSKMERNPINDQNLKGSSPSLGEGSSESYLSEVGMPHMWNNF*