Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG009611 | XP_024632959.1 | 73.611 | 216 | 41 | 4 | 1 | 205 | 1 | 211 | 1.34e-92 | 285 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG009611 | tr|A0A396JGH2|A0A396JGH2_MEDTR | 73.611 | 216 | 41 | 4 | 1 | 205 | 1 | 211 | 6.38e-93 | 285 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG009611 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG009611 | MtrunA17_Chr2g0331601 | 73.611 | 216 | 41 | 4 | 1 | 205 | 1 | 211 | 1.23e-96 | 285 |
| MsaG009611 | MtrunA17_Chr6g0481281 | 63.768 | 69 | 20 | 2 | 11 | 75 | 27 | 94 | 1.11e-18 | 82.8 |
| MsaG009611 | MtrunA17_Chr6g0481281 | 59.259 | 54 | 17 | 2 | 114 | 162 | 45 | 98 | 1.21e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATACAAGGTAGTTATAAT+AGG | 0.224337 | 2:-62336371 | MsaT009611.1:CDS |
| AAATAATACTATGGCTAGTT+TGG | 0.292025 | 2:-62336109 | MsaT009611.1:CDS |
| CATGATGAAGTTTCTGCTTA+AGG | 0.329845 | 2:+62336169 | None:intergenic |
| AATACAAGGAAATAATACTA+TGG | 0.401260 | 2:-62336118 | MsaT009611.1:CDS |
| TTTAGCAAAATAAAAGCATT+GGG | 0.424274 | 2:-62336213 | MsaT009611.1:CDS |
| AATACAAGGTAGTTATAATA+GGG | 0.431054 | 2:-62336370 | MsaT009611.1:CDS |
| ACACATCAATGCAATGTTTG+TGG | 0.441710 | 2:-62336243 | MsaT009611.1:CDS |
| TTTAGCAATGGAAAAGCATT+GGG | 0.444822 | 2:-62336465 | MsaT009611.1:CDS |
| TGAGAAAACCTATGAAGATT+CGG | 0.461549 | 2:-62336598 | MsaT009611.1:CDS |
| TTATTTCCTTGTATTGAGAA+AGG | 0.466446 | 2:+62336126 | None:intergenic |
| TATTTCCTTGTATTGAGAAA+GGG | 0.470601 | 2:+62336127 | None:intergenic |
| AGTCAGAAAAGAGGGTGAAA+AGG | 0.473192 | 2:-62336047 | MsaT009611.1:CDS |
| ACACATCAGTGCAATGTTTG+TGG | 0.475496 | 2:-62336495 | MsaT009611.1:CDS |
| ATTCGGAAAAGACGATGAAA+AGG | 0.477110 | 2:-62336581 | MsaT009611.1:CDS |
| CACATCAGTGCAATGTTTGT+GGG | 0.483344 | 2:-62336494 | MsaT009611.1:CDS |
| TTTGATAAAGATAGTAAGCA+TGG | 0.491925 | 2:-62336342 | MsaT009611.1:CDS |
| GATACCCCTTTCTCAATACA+AGG | 0.503509 | 2:-62336132 | MsaT009611.1:CDS |
| ACTTCATCATGATTCACAAA+AGG | 0.507116 | 2:-62336157 | MsaT009611.1:CDS |
| AACTACCTTGTATTGAGAGA+GGG | 0.509993 | 2:+62336379 | None:intergenic |
| AATTAATCATCGTTCACAAA+AGG | 0.518691 | 2:-62336409 | MsaT009611.1:CDS |
| GCTTAAGGAAGTGAGATCTT+CGG | 0.523012 | 2:+62336184 | None:intergenic |
| CAAACCCCTCTCTCAATACA+AGG | 0.526964 | 2:-62336384 | MsaT009611.1:CDS |
| AGAATCTACACATCAGTGCT+AGG | 0.545184 | 2:-62335968 | None:intergenic |
| TCTTAAGAAAGTGAGATCTC+CGG | 0.549197 | 2:+62336436 | None:intergenic |
| ATTTCCTTGTATTGAGAAAG+GGG | 0.553284 | 2:+62336128 | None:intergenic |
| GAAAAGCATTGGGTGGTCAC+CGG | 0.556634 | 2:-62336455 | MsaT009611.1:CDS |
| GAATGATGAAGATGATGCTA+AGG | 0.562831 | 2:-62336289 | MsaT009611.1:CDS |
| CTATGAAGAGTCAGAAAAGA+GGG | 0.570873 | 2:-62336055 | MsaT009611.1:CDS |
| CCTATGAAGAGTCAGAAAAG+AGG | 0.578028 | 2:-62336056 | MsaT009611.1:CDS |
| AAGCGATGAAGATGATGCTA+AGG | 0.582770 | 2:-62336007 | MsaT009611.1:CDS |
| TGAAGATGATGCTAAGGATG+AGG | 0.590398 | 2:-62336001 | MsaT009611.1:CDS |
| ACTACCTTGTATTGAGAGAG+GGG | 0.593179 | 2:+62336380 | None:intergenic |
| TAACTACCTTGTATTGAGAG+AGG | 0.601425 | 2:+62336378 | None:intergenic |
| AGCAAAATAAAAGCATTGGG+TGG | 0.607881 | 2:-62336210 | MsaT009611.1:CDS |
| TAGTGATGAAGATGATGCTA+AGG | 0.613974 | 2:-62336541 | MsaT009611.1:CDS |
| AGCAATGGAAAAGCATTGGG+TGG | 0.615604 | 2:-62336462 | MsaT009611.1:CDS |
| ATGGATAAAGATCATAAGCA+TGG | 0.623190 | 2:-62336624 | MsaT009611.1:CDS |
| TGAAGATGATGCTAAGGATG+AGG | 0.644459 | 2:-62336535 | MsaT009611.1:CDS |
| AGATCATAAGCATGATCACG+AGG | 0.690275 | 2:-62336082 | MsaT009611.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 62335969 | 62336646 | 62335969 | ID=MsaG009611 |
| Chr2 | mRNA | 62335969 | 62336646 | 62335969 | ID=MsaT009611.1;Parent=MsaG009611 |
| Chr2 | exon | 62335969 | 62336646 | 62335969 | ID=MsaT009611.1.exon1;Parent=MsaT009611.1 |
| Chr2 | CDS | 62335969 | 62336646 | 62335969 | ID=cds.MsaT009611.1;Parent=MsaT009611.1 |
| Gene Sequence |
| Protein sequence |
