AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG009647


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009647 MtrunA17_Chr2g0315651 92.098 367 29 0 1 367 1 367 0.0 715
MsaG009647 MtrunA17_Chr2g0315641 74.462 372 86 5 1 367 4 371 0.0 561
MsaG009647 MtrunA17_Chr2g0286441 47.500 360 155 7 37 367 39 393 4.88e-105 314
MsaG009647 MtrunA17_Chr5g0404321 46.995 366 155 9 37 367 55 416 3.24e-102 308
MsaG009647 MtrunA17_Chr8g0373001 46.133 375 167 6 25 367 41 412 7.05e-102 306
MsaG009647 MtrunA17_Chr1g0196071 45.983 361 157 9 37 367 50 402 1.01e-96 293
MsaG009647 MtrunA17_Chr1g0201891 43.864 383 159 10 37 367 53 431 2.64e-95 290
MsaG009647 MtrunA17_Chr1g0155301 45.938 357 163 9 37 367 70 422 2.54e-93 285
MsaG009647 MtrunA17_Chr2g0278511 42.744 379 164 10 37 367 54 427 3.90e-92 282
MsaG009647 MtrunA17_Chr8g0373361 37.838 370 186 8 25 360 46 405 6.04e-68 219
MsaG009647 MtrunA17_Chr8g0373331 38.310 355 154 10 25 360 40 348 8.05e-55 183
MsaG009647 MtrunA17_Chr8g0373341 36.443 343 130 11 34 357 47 320 7.57e-39 141
MsaG009647 MtrunA17_Chr1g0156151 46.429 140 71 1 52 187 66 205 5.33e-34 125
MsaG009647 MtrunA17_Chr8g0373351 35.088 285 122 10 91 359 49 286 4.44e-27 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG009647 AT1G53320.1 62.500 384 122 7 1 367 1 379 1.52e-158 450
MsaG009647 AT2G18280.2 46.875 352 166 5 34 367 45 393 2.14e-102 308
MsaG009647 AT2G18280.1 46.875 352 166 5 34 367 45 393 2.14e-102 308
MsaG009647 AT2G47900.2 43.611 360 169 6 37 367 52 406 5.14e-100 302
MsaG009647 AT2G47900.1 43.611 360 169 6 37 367 52 406 5.14e-100 302
MsaG009647 AT2G47900.3 43.490 361 169 6 37 367 52 407 1.06e-99 301
MsaG009647 AT1G76900.2 40.139 431 174 9 18 367 28 455 1.40e-97 297
MsaG009647 AT1G76900.1 40.139 431 174 9 18 367 28 455 1.40e-97 297
MsaG009647 AT1G76900.3 40.139 431 174 9 18 367 28 455 1.40e-97 297
MsaG009647 AT3G06380.1 44.751 362 167 9 31 367 27 380 6.04e-94 286
MsaG009647 AT1G25280.1 42.199 391 162 10 37 367 59 445 7.74e-92 282
MsaG009647 AT1G47270.3 44.538 357 168 9 33 367 40 388 2.11e-90 277
MsaG009647 AT1G47270.1 44.538 357 168 9 33 367 65 413 2.33e-90 278
MsaG009647 AT1G47270.2 44.538 357 168 9 33 367 64 412 3.06e-90 277
MsaG009647 AT5G18680.1 43.164 373 171 10 25 367 28 389 7.30e-88 270
MsaG009647 AT1G43640.1 40.674 386 167 11 35 367 53 429 9.80e-85 264
MsaG009647 AT1G43640.2 40.860 372 158 11 49 367 1 363 4.59e-81 252
MsaG009647 AT3G06380.2 43.151 292 134 8 100 367 5 288 3.04e-67 214
MsaG009647 AT5G18680.2 42.475 299 133 8 98 367 9 297 4.68e-65 209
MsaG009647 AT1G25280.3 39.114 271 102 9 156 367 1 267 1.09e-48 165
MsaG009647 AT1G25280.2 39.114 271 102 9 156 367 1 267 1.09e-48 165
MsaG009647 AT1G61940.2 48.276 87 45 0 40 126 1 87 2.00e-22 95.9
MsaG009647 AT1G61940.1 49.180 61 31 0 40 100 1 61 1.69e-14 72.8

Find 87 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCACCACTGTGTTGTAAAT+TGG 0.184549 2:+62757088 MsaT009647.1:CDS
ATCATTCGCCTTGGACGTTT+TGG 0.253223 2:-62758015 None:intergenic
TCGCCAGCATTAACAGATAA+AGG 0.277525 2:+62757305 MsaT009647.1:intron
CTCTCCCTCCTGGTTGGCTT+TGG 0.281810 2:-62758269 None:intergenic
ATGATATCCGCAAGAAGTTC+TGG 0.302123 2:-62756412 None:intergenic
CGGGTAGCTCTGCTAGTTGA+TGG 0.323356 2:-62757901 None:intergenic
TGGGGAAGGTCGCGTGGCCC+TGG 0.326133 2:-62757069 None:intergenic
TTTCCCTTCTTGTCTTAAAC+AGG 0.347974 2:+62756567 MsaT009647.1:CDS
GATATCTTCTAGCTGCTAAT+AGG 0.356446 2:-62757334 None:intergenic
AACTAGCTTCGGCACTAAAC+TGG 0.357473 2:+62758398 MsaT009647.1:CDS
GCCATTTGCTTAACTAGCTT+CGG 0.361202 2:+62758387 MsaT009647.1:CDS
CCTACCTCGACGTTGCTAAC+AGG 0.373881 2:-62757958 None:intergenic
TCTTCATCTCCTCTCCCTCC+TGG 0.374907 2:-62758279 None:intergenic
GAACGCATGAAAGGCGGATA+AGG 0.389134 2:-62758364 None:intergenic
AGCTCTGCTAGTTGATGGTT+TGG 0.396144 2:-62757896 None:intergenic
CAAAGCCAACCAGGAGGGAG+AGG 0.411585 2:+62758270 MsaT009647.1:CDS
CGCCAGCATTAACAGATAAA+GGG 0.417908 2:+62757306 MsaT009647.1:intron
GTACTTACTTACATGGTGTA+AGG 0.424832 2:-62757146 None:intergenic
TCTGTTTGCTTGCAAATCGT+CGG 0.458826 2:-62757921 None:intergenic
GTAGGGCCACCTTCTCTTAA+CGG 0.460650 2:+62756535 MsaT009647.1:CDS
CTGTCATATCCTTTCTCTCA+TGG 0.461057 2:-62758206 None:intergenic
CTTGAACTTCCATGAGAGAA+AGG 0.465557 2:+62758197 MsaT009647.1:CDS
TTGATGGTTTGGCACCATCC+TGG 0.472921 2:-62757885 None:intergenic
AACGCATGAAAGGCGGATAA+GGG 0.474762 2:-62758363 None:intergenic
CAAGGAAGTAATGCTTATGT+TGG 0.475540 2:+62757410 MsaT009647.1:CDS
CATCTCCTCTCCCTCCTGGT+TGG 0.480773 2:-62758275 None:intergenic
TATTAGCAGCTAGAAGATAT+CGG 0.489255 2:+62757336 MsaT009647.1:CDS
CTTGTGCCTGCGTTTGCAAA+CGG 0.489375 2:+62756488 MsaT009647.1:CDS
CGTTGCTAACAGGAACTTGA+GGG 0.495414 2:-62757948 None:intergenic
CTGTTTGCTTGCAAATCGTC+GGG 0.497701 2:-62757920 None:intergenic
TTTGTCCTCCGATGCACTAC+CGG 0.499562 2:-62756368 None:intergenic
CTTTCGCATATTGATGAGGC+AGG 0.503626 2:-62758054 None:intergenic
CCAATTTACAACACAGTGGT+GGG 0.506056 2:-62757088 None:intergenic
GCCGAAGCTAGTTAAGCAAA+TGG 0.515952 2:-62758388 None:intergenic
AGAGAGCAAATCATTCGCCT+TGG 0.519881 2:-62758024 None:intergenic
ATCAGTGAAGAACTTTCAGC+TGG 0.522886 2:+62758233 MsaT009647.1:CDS
CAGATACTTCAACAATGCTC+AGG 0.525873 2:+62756318 MsaT009647.1:CDS
AGGACCTGTTTAAGACAAGA+AGG 0.526652 2:-62756571 None:intergenic
ATGCTTATGTTGGCAAGCTA+AGG 0.535171 2:+62757420 MsaT009647.1:CDS
ATTAAACGAGTTGATGTCAC+TGG 0.535809 2:+62756433 MsaT009647.1:CDS
TCCAATTTACAACACAGTGG+TGG 0.539488 2:-62757089 None:intergenic
CATCGGAGGACAAAACTGAT+TGG 0.540142 2:+62756377 MsaT009647.1:CDS
ATATCACACGTGAGGTCGTA+GGG 0.543247 2:+62756518 MsaT009647.1:CDS
CGCAAGAAGTTCTGGAAGCA+TGG 0.545732 2:-62756404 None:intergenic
GCTGTCGTAAATTGTGAAGT+TGG 0.553594 2:-62757863 None:intergenic
GGAACGAGCAATTAGAGTGC+TGG 0.557590 2:+62758172 MsaT009647.1:CDS
GGCAAGATAGAGGTAAAATG+TGG 0.562187 2:-62757124 None:intergenic
CTTTCAGCTGGTTGCCACTG+TGG 0.563933 2:+62758245 MsaT009647.1:CDS
GCAAATGGCGAACGCATGAA+AGG 0.564410 2:-62758373 None:intergenic
CCTGTTAGCAACGTCGAGGT+AGG 0.569841 2:+62757958 MsaT009647.1:CDS
ACTGTGGACCAAAGCCAACC+AGG 0.574474 2:+62758261 MsaT009647.1:CDS
TTTATTCTTCAAGATTGTGT+AGG 0.575970 2:-62758139 None:intergenic
CAGTGGTGGGGAAGGTCGCG+TGG 0.579862 2:-62757075 None:intergenic
TACTTACTTACATGGTGTAA+GGG 0.588591 2:-62757145 None:intergenic
GATATCACACGTGAGGTCGT+AGG 0.603348 2:+62756517 MsaT009647.1:CDS
CACCACCGGTAGTGCATCGG+AGG 0.607308 2:+62756363 MsaT009647.1:CDS
TGACACCACCGGTAGTGCAT+CGG 0.608180 2:+62756360 MsaT009647.1:CDS
CTTCACAATTTACGACAGCC+AGG 0.609339 2:+62757867 MsaT009647.1:CDS
GTGCCTGCGTTTGCAAACGG+TGG 0.610120 2:+62756491 MsaT009647.1:CDS
ACAATTTACGACAGCCAGGA+TGG 0.612721 2:+62757871 MsaT009647.1:CDS
TTACAACACAGTGGTGGGGA+AGG 0.615695 2:-62757083 None:intergenic
ACGTTGCTAACAGGAACTTG+AGG 0.623409 2:-62757949 None:intergenic
ATGGTGTAAGGGCAAGATAG+AGG 0.623484 2:-62757134 None:intergenic
CATGCTTCCAGAACTTCTTG+CGG 0.624235 2:+62756405 MsaT009647.1:CDS
CAATTTACAACACAGTGGTG+GGG 0.625248 2:-62757087 None:intergenic
GGACCTGTTTAAGACAAGAA+GGG 0.625907 2:-62756570 None:intergenic
TGAACTTTCGCATATTGATG+AGG 0.626688 2:-62758058 None:intergenic
GTCCTCCGATGCACTACCGG+TGG 0.634176 2:-62756365 None:intergenic
TGAAGAATAAAGCTCCGATG+TGG 0.636117 2:+62758151 MsaT009647.1:CDS
TCTCCACCGTTTGCAAACGC+AGG 0.641600 2:-62756494 None:intergenic
GCTCAGGCTCTCAATGCTAC+CGG 0.643110 2:+62756334 MsaT009647.1:CDS
GCTACCGGCGATGACACCAC+CGG 0.645253 2:+62756349 MsaT009647.1:CDS
ACTACCGGTGGTGTCATCGC+CGG 0.646189 2:-62756353 None:intergenic
ATCGAGCGACATAACATACT+CGG 0.647174 2:-62757372 None:intergenic
TGGTTGGCTTTGGTCCACAG+TGG 0.652104 2:-62758259 None:intergenic
TCTAATTGCTCGTTCCACAT+CGG 0.654420 2:-62758165 None:intergenic
AAGATAGAGGTAAAATGTGG+AGG 0.660216 2:-62757121 None:intergenic
TGGACCAAAGCCAACCAGGA+GGG 0.665639 2:+62758265 MsaT009647.1:CDS
AATGGCGAACGCATGAAAGG+CGG 0.674797 2:-62758370 None:intergenic
TAAGGGCTGTCTATAGCACA+TGG 0.676456 2:-62758346 None:intergenic
GCGACGACATTTCGACGATG+CGG 0.691215 2:-62756466 None:intergenic
TATTCCAATTTACAACACAG+TGG 0.695058 2:-62757092 None:intergenic
GTGGAGAGATATCACACGTG+AGG 0.702332 2:+62756510 MsaT009647.1:CDS
AGTTCCTGTTAGCAACGTCG+AGG 0.712433 2:+62757954 MsaT009647.1:CDS
TGAGAGAAAGGATATGACAG+TGG 0.718707 2:+62758209 MsaT009647.1:CDS
TTGATGTCACTGGAGAACAA+TGG 0.719375 2:+62756443 MsaT009647.1:CDS
GTGGACCAAAGCCAACCAGG+AGG 0.733661 2:+62758264 MsaT009647.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 62756289 62758431 62756289 ID=MsaG009647
Chr2 mRNA 62756289 62758431 62756289 ID=MsaT009647.1;Parent=MsaG009647
Chr2 exon 62756289 62756588 62756289 ID=MsaT009647.1.exon1;Parent=MsaT009647.1
Chr2 CDS 62756289 62756588 62756289 ID=cds.MsaT009647.1;Parent=MsaT009647.1
Chr2 exon 62757069 62757156 62757069 ID=MsaT009647.1.exon2;Parent=MsaT009647.1
Chr2 CDS 62757069 62757156 62757069 ID=cds.MsaT009647.1;Parent=MsaT009647.1
Chr2 exon 62757312 62757441 62757312 ID=MsaT009647.1.exon3;Parent=MsaT009647.1
Chr2 CDS 62757312 62757441 62757312 ID=cds.MsaT009647.1;Parent=MsaT009647.1
Chr2 exon 62757846 62758431 62757846 ID=MsaT009647.1.exon4;Parent=MsaT009647.1
Chr2 CDS 62757846 62758431 62757846 ID=cds.MsaT009647.1;Parent=MsaT009647.1
Gene Sequence

>MsaT009647.1

ATGAAGCTCTTTCGATCTCTTAAATTCTTCAGATACTTCAACAATGCTCAGGCTCTCAATGCTACCGGCGATGACACCACCGGTAGTGCATCGGAGGACAAAACTGATTGGTCTACCATGCTTCCAGAACTTCTTGCGGATATCATTAAACGAGTTGATGTCACTGGAGAACAATGGCCGCATCGTCGAAATGTCGTCGCTTGTGCCTGCGTTTGCAAACGGTGGAGAGATATCACACGTGAGGTCGTAGGGCCACCTTCTCTTAACGGAAAAATCACTTTCCCTTCTTGTCTTAAACAGCCAGGGCCACGCGACCTTCCCCACCACTGTGTTGTAAATTGGAATAAGAAGACCTCCACATTTTACCTCTATCTTGCCCTTACACCATCATTAACAGATAAAGGGAAGTTCCTATTAGCAGCTAGAAGATATCGGTGTGTTACACATACCGAGTATGTTATGTCGCTCGATGCTGATGACTTTTCACAAGGAAGTAATGCTTATGTTGGCAAGCTAAGCTCAGATTTTCTTGAAGCCAACTTCACAATTTACGACAGCCAGGATGGTGCCAAACCATCAACTAGCAGAGCTACCCGACGATTTGCAAGCAAACAGATAAGCCCTCAAGTTCCTGTTAGCAACGTCGAGGTAGGAAACATCTCCTACAAAAAGTTTAGCCTTTTGAAACCAAAACGTCCAAGGCGAATGATTTGCTCTCTGAAGTCTCCTGCCTCATCAATATGCGAAAGTTCAGACAGCAAGTCTTTCGATCAACATACGATGCACTGTAAAGAACAAGCTACTTCTTCTGCCTACACAATCTTGAAGAATAAAGCTCCGATGTGGAACGAGCAATTAGAGTGCTGGTTCTTGAACTTCCATGAGAGAAAGGATATGACAGTGGCATCAGTGAAGAACTTTCAGCTGGTTGCCACTGTGGACCAAAGCCAACCAGGAGGGAGAGGAGATGAAGAAACAGTTCTTCTCCAGTTTTGTAAAGTAGGGGGTGATACTTTCACCATGTGCTATAGACAGCCCTTATCCGCCTTTCATGCGTTCGCCATTTGCTTAACTAGCTTCGGCACTAAACTGGCTTGTCAGTAA

Protein sequence

>MsaT009647.1

MKLFRSLKFFRYFNNAQALNATGDDTTGSASEDKTDWSTMLPELLADIIKRVDVTGEQWPHRRNVVACACVCKRWRDITREVVGPPSLNGKITFPSCLKQPGPRDLPHHCVVNWNKKTSTFYLYLALTPSLTDKGKFLLAARRYRCVTHTEYVMSLDADDFSQGSNAYVGKLSSDFLEANFTIYDSQDGAKPSTSRATRRFASKQISPQVPVSNVEVGNISYKKFSLLKPKRPRRMICSLKSPASSICESSDSKSFDQHTMHCKEQATSSAYTILKNKAPMWNEQLECWFLNFHERKDMTVASVKNFQLVATVDQSQPGGRGDEETVLLQFCKVGGDTFTMCYRQPLSAFHAFAICLTSFGTKLACQ*