AlfalfaGEDB | MsaG009912

M. sativa ssp. caerulea / MsaG009912

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007275 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010374 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0090558 GO:0090627 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009912	AES66736.2	88.770	187	21	0	2	188	175	361	3.12e-113	337

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009912	sp\|Q9LSE2\|ICE1_ARATH	55.080	187	84	0	1	187	304	490	2.09e-66	214

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009912	tr\|G7IR55\|G7IR55_MEDTR	88.770	187	21	0	2	188	175	361	1.49e-113	337

TFs/TRs:

Gene ID	Type	Classification
MsaG009912	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009912	MtrunA17_Chr7g0252041	55.263	190	83	1	1	188	283	472	3.74e-64	204
MsaG009912	MtrunA17_Chr3g0131691	53.191	188	87	1	1	188	295	481	4.02e-64	204
MsaG009912	MtrunA17_Chr3g0089131	80.000	60	12	0	107	166	5	64	1.89e-30	107
MsaG009912	MtrunA17_Chr5g0411541	39.247	186	94	3	2	187	154	320	1.02e-28	108
MsaG009912	MtrunA17_Chr3g0089121	65.432	81	23	1	33	113	1	76	7.51e-28	100
MsaG009912	MtrunA17_Chr3g0141091	35.829	187	94	4	2	187	171	332	1.49e-26	103
MsaG009912	MtrunA17_Chr1g0211081	31.863	204	110	5	3	183	316	513	5.15e-25	100
MsaG009912	MtrunA17_Chr3g0135471	88.889	45	5	0	122	166	1	45	3.49e-22	85.1
MsaG009912	MtrunA17_Chr8g0365711	32.086	187	106	5	3	182	243	415	1.04e-19	85.5
MsaG009912	MtrunA17_Chr2g0318461	82.222	45	8	0	122	166	1	45	9.13e-19	76.3
MsaG009912	MtrunA17_Chr8g0346151	31.646	158	100	3	2	158	277	427	7.70e-16	74.7
MsaG009912	MtrunA17_Chr2g0320291	31.351	185	111	7	3	185	132	302	1.09e-15	73.6
MsaG009912	MtrunA17_Chr8g0346161	32.258	155	94	3	2	155	289	433	2.06e-14	70.5
MsaG009912	MtrunA17_Chr4g0028071	28.108	185	121	5	3	185	148	322	4.33e-14	69.3
MsaG009912	MtrunA17_Chr2g0298751	26.984	189	121	4	3	181	51	232	1.71e-13	66.6
MsaG009912	MtrunA17_Chr8g0386921	31.250	160	88	5	6	146	408	564	4.29e-13	66.6
MsaG009912	MtrunA17_Chr2g0318471	80.952	42	6	1	2	43	175	214	1.42e-12	63.9
MsaG009912	MtrunA17_Chr3g0135481	93.750	32	2	0	2	33	197	228	1.45e-12	63.9
MsaG009912	MtrunA17_Chr5g0400671	26.667	180	119	3	6	185	156	322	9.21e-12	62.4
MsaG009912	MtrunA17_Chr8g0366751	30.921	152	104	1	8	158	466	617	9.45e-12	62.8
MsaG009912	MtrunA17_Chr1g0185621	30.769	156	84	3	6	137	477	632	1.03e-11	62.8
MsaG009912	MtrunA17_Chr7g0225901	28.481	158	97	3	6	160	90	234	1.09e-11	61.6
MsaG009912	MtrunA17_Chr7g0275821	52.727	55	26	0	8	62	256	310	4.11e-11	60.8
MsaG009912	MtrunA17_Chr2g0280161	26.994	163	94	4	6	160	179	324	6.12e-11	60.1
MsaG009912	MtrunA17_Chr4g0049331	26.786	168	97	3	1	160	132	281	8.09e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG009912	AT3G26744.1	55.080	187	84	0	1	187	304	490	2.12e-67	214
MsaG009912	AT3G26744.4	55.080	187	84	0	1	187	304	490	2.12e-67	214
MsaG009912	AT3G26744.2	55.080	187	84	0	1	187	304	490	2.12e-67	214
MsaG009912	AT1G12860.1	54.787	188	79	2	1	187	264	446	1.35e-64	205
MsaG009912	AT3G26744.6	57.927	164	69	0	1	164	304	467	2.98e-61	197
MsaG009912	AT1G12860.2	56.970	165	65	2	1	164	264	423	5.82e-58	187
MsaG009912	AT2G16910.1	38.462	195	103	6	3	183	313	504	1.52e-28	111
MsaG009912	AT5G10570.1	38.298	188	88	5	2	187	149	310	2.82e-25	99.8
MsaG009912	AT5G65640.1	37.097	186	102	3	2	187	176	346	5.30e-25	99.8
MsaG009912	AT1G63650.3	30.636	173	91	3	7	160	408	570	9.81e-15	72.0
MsaG009912	AT1G63650.1	30.636	173	91	3	7	160	408	570	9.81e-15	72.0
MsaG009912	AT1G63650.2	30.636	173	91	3	7	160	408	570	9.81e-15	72.0
MsaG009912	AT4G29930.6	29.221	154	88	3	3	153	53	188	2.87e-14	68.9
MsaG009912	AT2G28160.1	32.051	156	87	5	3	152	130	272	1.09e-13	68.2
MsaG009912	AT2G28160.2	32.051	156	87	5	3	152	130	272	1.27e-13	68.2
MsaG009912	AT4G29930.5	29.605	152	87	3	3	153	53	185	1.29e-13	67.0
MsaG009912	AT4G29930.2	29.605	152	87	3	3	153	53	185	1.51e-13	67.0
MsaG009912	AT5G57150.1	29.834	181	118	4	3	179	53	228	1.91e-13	67.0
MsaG009912	AT5G46760.1	31.210	157	102	2	2	158	413	563	2.75e-13	67.8
MsaG009912	AT5G65640.2	60.345	58	23	0	2	59	176	233	2.96e-13	67.0
MsaG009912	AT4G17880.1	30.556	180	113	4	2	181	414	581	3.19e-13	67.4
MsaG009912	AT5G41315.2	29.843	191	118	6	7	183	452	640	8.00e-13	66.6
MsaG009912	AT5G41315.1	29.843	191	118	6	7	183	444	632	8.45e-13	66.2
MsaG009912	AT5G41315.3	29.843	191	118	6	7	183	449	637	8.88e-13	66.2
MsaG009912	AT5G57150.5	49.254	67	32	1	3	67	53	119	1.27e-12	64.3
MsaG009912	AT5G57150.3	51.724	58	28	0	3	60	54	111	1.45e-12	63.9
MsaG009912	AT5G57150.2	51.724	58	28	0	3	60	54	111	1.64e-12	63.9
MsaG009912	AT5G57150.4	49.254	67	32	1	3	67	54	120	1.74e-12	64.3
MsaG009912	AT4G29930.1	28.313	166	100	3	3	153	53	214	1.81e-12	64.3
MsaG009912	AT4G29930.3	28.313	166	100	3	3	153	53	214	2.19e-12	64.3
MsaG009912	AT4G29930.7	28.313	166	100	3	3	153	53	214	2.19e-12	64.3
MsaG009912	AT4G29930.4	48.276	58	30	0	3	60	53	110	4.27e-12	62.4
MsaG009912	AT4G00870.1	48.148	54	28	0	8	61	253	306	1.99e-11	62.4
MsaG009912	AT2G46510.1	53.846	52	24	0	8	59	399	450	2.97e-11	62.0

Find 40 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGATACCTTGGTAATCTT+TGG	0.228828	2:+66205300	None:intergenic
GGTATTTGCAGGTAGATGTT+AGG	0.324730	2:-66200571	MsaT009912.1:intron
TGGATAAAATTTCAATACTT+AGG	0.367751	2:-66201923	MsaT009912.1:CDS
AACTCTTCCTTCATTTGAAC+TGG	0.372973	2:+66201765	None:intergenic
TCAGCGATGAGACTCCTTGA+TGG	0.376485	2:+66205378	None:intergenic
AAATAAGTACCTTTGTTGAT+TGG	0.383387	2:+66201712	None:intergenic
GTTTGCAAGCACAAAACATA+TGG	0.423414	2:+66200522	None:intergenic
TGCTTGCAAACCAGGTGTTT+TGG	0.426101	2:-66200510	MsaT009912.1:CDS
GGATAAAATTTCAATACTTA+GGG	0.426628	2:-66201922	MsaT009912.1:CDS
CAATACTGTTTCAATTTGCT+CGG	0.440036	2:+66199662	None:intergenic
GGCATTAGATAGCCTTGGAT+TGG	0.455224	2:-66200474	MsaT009912.1:CDS
GATGTTAGGGTAAGGGAAGG+AGG	0.466243	2:-66200557	MsaT009912.1:CDS
GTATTTGCAGGTAGATGTTA+GGG	0.469646	2:-66200570	MsaT009912.1:intron
TGCAGGTAGATGTTAGGGTA+AGG	0.474740	2:-66200565	MsaT009912.1:intron
GCAGGTAGATGTTAGGGTAA+GGG	0.478930	2:-66200564	MsaT009912.1:intron
AATTGAAACAGTATTGTTGA+AGG	0.479928	2:-66199655	MsaT009912.1:CDS
ATGAAGGCATTAGATAGCCT+TGG	0.485524	2:-66200479	MsaT009912.1:CDS
CTTCCTTCATTTGAACTGGA+AGG	0.486436	2:+66201769	None:intergenic
AATACTGTTTCAATTTGCTC+GGG	0.488684	2:+66199663	None:intergenic
CCTTGGATTGGATGTTCACC+AGG	0.489700	2:-66200462	MsaT009912.1:CDS
TTATGTCTTAACAATGTGTC+AGG	0.494453	2:-66201744	MsaT009912.1:CDS
TTCCTTCATTTGAACTGGAA+GGG	0.498449	2:+66201770	None:intergenic
TGAAACAACTTATGTTAGCC+TGG	0.501626	2:+66200444	None:intergenic
AACCCTTCCAGTTCAAATGA+AGG	0.531349	2:-66201772	MsaT009912.1:CDS
TCATTTATGCCACCACCTAC+AGG	0.540438	2:-66201822	MsaT009912.1:CDS
TTTAGATGTGTTCAAAGCTG+AGG	0.570390	2:-66200411	MsaT009912.1:intron
ATAGTCGACGCCAAAACACC+TGG	0.570568	2:+66200500	None:intergenic
ATGTGAGTTCCTGTAGGTGG+TGG	0.599474	2:+66201813	None:intergenic
GTCTTAATGTGAGTTCCTGT+AGG	0.604037	2:+66201807	None:intergenic
GTAGATGTTAGGGTAAGGGA+AGG	0.609192	2:-66200560	MsaT009912.1:CDS
GGTGGTGGCATAAATGATGT+AGG	0.613047	2:+66201828	None:intergenic
CCTGGTGAACATCCAATCCA+AGG	0.613572	2:+66200462	None:intergenic
GGATGCTATTGACTACTCAA+AGG	0.617862	2:-66201901	MsaT009912.1:CDS
AACTGGAAGGGTTGACATGG+TGG	0.620781	2:+66201782	None:intergenic
ATACTGTTTCAATTTGCTCG+GGG	0.623668	2:+66199664	None:intergenic
TCATCGCTGAAAAGAAGCGA+AGG	0.628159	2:-66205365	MsaT009912.1:CDS
TGTTGTTCCAAAGATTACCA+AGG	0.639572	2:-66205307	MsaT009912.1:intron
TACTGTTTCAATTTGCTCGG+GGG	0.641355	2:+66199665	None:intergenic
TTGAACTGGAAGGGTTGACA+TGG	0.641549	2:+66201779	None:intergenic
TTAATGTGAGTTCCTGTAGG+TGG	0.646819	2:+66201810	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	66199638	66205403	66199638	ID=MsaG009912
Chr2	mRNA	66199638	66205403	66199638	ID=MsaT009912.1;Parent=MsaG009912
Chr2	exon	66199638	66199712	66199638	ID=MsaT009912.1.exon4;Parent=MsaT009912.1
Chr2	CDS	66199638	66199712	66199638	ID=cds.MsaT009912.1;Parent=MsaT009912.1
Chr2	exon	66200412	66200582	66200412	ID=MsaT009912.1.exon3;Parent=MsaT009912.1
Chr2	CDS	66200412	66200582	66200412	ID=cds.MsaT009912.1;Parent=MsaT009912.1
Chr2	exon	66201722	66201946	66201722	ID=MsaT009912.1.exon2;Parent=MsaT009912.1
Chr2	CDS	66201722	66201946	66201722	ID=cds.MsaT009912.1;Parent=MsaT009912.1
Chr2	exon	66205308	66205403	66205308	ID=MsaT009912.1.exon1;Parent=MsaT009912.1
Chr2	CDS	66205308	66205403	66205308	ID=cds.MsaT009912.1;Parent=MsaT009912.1

Gene Sequence

>MsaT009912.1

ATGCCATCAAGGAGTCTCATCGCTGAAAAGAAGCGAAGGCAGAAACTCAGCGATAATATGCACAAGCTTCGTTCTGTTGTTCCAAAGATTACCAAGATGGATAAAATTTCAATACTTAGGGATGCTATTGACTACTCAAAGGAGTTACAAAAACAAATTAATGATCTTCAAAGTGAGATAGAGTCAAGCTCTCCTACATCATTTATGCCACCACCTACAGGAACTCACATTAAGACATCCACCATGTCAACCCTTCCAGTTCAAATGAAGGAAGAGTTATGTCTTAACAATGTGTCAGGTCTTAAAAACCAATCAACAAAGGTAGATGTTAGGGTAAGGGAAGGAGGAATTGTCAACATCCATATGTTTTGTGCTTGCAAACCAGGTGTTTTGGCGTCGACTATGAAGGCATTAGATAGCCTTGGATTGGATGTTCACCAGGCTAACATAAGTTGTTTCAATGATTTTTGTTTAGATGTGTTCAAAGCTGAGCAACACAACAAAGACCAAGAATTGACCCCCGAGCAAATTGAAACAGTATTGTTGAAGGCATTGAGTTTTCATTGA

Protein sequence

>MsaT009912.1

MPSRSLIAEKKRRQKLSDNMHKLRSVVPKITKMDKISILRDAIDYSKELQKQINDLQSEIESSSPTSFMPPPTGTHIKTSTMSTLPVQMKEELCLNNVSGLKNQSTKVDVRVREGGIVNIHMFCACKPGVLASTMKALDSLGLDVHQANISCFNDFCLDVFKAEQHNKDQELTPEQIETVLLKALSFH*